Bioactive Materials最新文献_第3页

Advances in biomaterial-based tissue engineering for peripheral nerve injury repair 基于生物材料的周围神经损伤修复组织工程研究进展。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-13 DOI: 10.1016/j.bioactmat.2024.12.005

Xinlei Yao , Tong Xue , Bingqian Chen , Xinyang Zhou , Yanan Ji , Zihui Gao , Boya Liu , Jiawen Yang , Yuntian Shen , Hualin Sun , Xiaosong Gu , Bin Dai

{"title":"Advances in biomaterial-based tissue engineering for peripheral nerve injury repair","authors":"Xinlei Yao , Tong Xue , Bingqian Chen , Xinyang Zhou , Yanan Ji , Zihui Gao , Boya Liu , Jiawen Yang , Yuntian Shen , Hualin Sun , Xiaosong Gu , Bin Dai","doi":"10.1016/j.bioactmat.2024.12.005","DOIUrl":"10.1016/j.bioactmat.2024.12.005","url":null,"abstract":"<div><div>Peripheral nerve injury is a common clinical disease. Effective post-injury nerve repair remains a challenge in neurosurgery, and clinical outcomes are often unsatisfactory, resulting in social and economic burden. Particularly, the repair of long-distance nerve defects remains a challenge. The existing nerve transplantation strategies show limitations, including donor site morbidity and immune rejection issues. The multiple studies have revealed the potential of tissue engineering strategies based on biomaterials in the repair of peripheral nerve injuries. We review the events of regeneration after peripheral nerve injury, evaluates the efficacy of existing nerve grafting strategies, and delves into the progress in the construction and application strategies of different nerve guidance conduits. A spotlight is cast on the materials, technologies, seed cells, and microenvironment within these conduits to facilitate optimal nerve regeneration. Further discussion was conducted on the approve of nerve guidance conduits and potential future research directions. This study anticipates and proposes potential avenues for future research, aiming to refine existing strategies and uncover innovative approaches in biomaterial-based nerve repair. This study endeavors to synthesize the collective insights from the fields of neuroscience, materials science, and regenerative medicine, offering a multifaceted perspective on the role of biomaterials in advancing the frontiers of peripheral nerve injury treatment.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 150-172"},"PeriodicalIF":18.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinspired conductive oriented nanofiber felt with efficient ROS clearance and anti-inflammation for inducing M2 macrophage polarization and accelerating spinal cord injury repair 生物启发导电定向纳米纤维毡具有有效的ROS清除和抗炎症诱导M2巨噬细胞极化和加速脊髓损伤修复。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-13 DOI: 10.1016/j.bioactmat.2024.12.009

Qingxia Zhang , Jiahe Zheng , Linlong Li , Jui-Ming Yeh , Xianrui Xie , Yuqing Zhao , Chengbo Li , Guige Hou , Huanhuan Yan

{"title":"Bioinspired conductive oriented nanofiber felt with efficient ROS clearance and anti-inflammation for inducing M2 macrophage polarization and accelerating spinal cord injury repair","authors":"Qingxia Zhang , Jiahe Zheng , Linlong Li , Jui-Ming Yeh , Xianrui Xie , Yuqing Zhao , Chengbo Li , Guige Hou , Huanhuan Yan","doi":"10.1016/j.bioactmat.2024.12.009","DOIUrl":"10.1016/j.bioactmat.2024.12.009","url":null,"abstract":"<div><div>Complete spinal cord injury (SCI) causes permanent locomotor, sensory and neurological dysfunctions. Targeting complex immunopathological microenvironment at SCI sites comprising inflammatory cytokines infiltration, oxidative stress and massive neuronal apoptosis, the conductive oriented nanofiber felt with efficient ROS clearance, anti-inflammatory effect and accelerating neural regeneration is constructed by step-growth addition polymerization and electrostatic spinning technique for SCI repair. The formation of innovative Fe<sup>3+</sup>-PDA-PAT chelate in nanofiber felt enhances hydrophilic, antioxidant, antibacterial, hemostatic and binding factor capacities, thereby regulating immune microenvironment of SCI. With the capabilities of up-regulating COX5A and STAT6 expressions, down-regulating the expressions of IL1β, CD36, p-ERK, NFκB2 and NFκB signaling pathway proteins, the nanofiber felt attenuates oxidative stress injury, promotes M2 macrophage polarization and down-regulates inflammatory response. After implantation into complete transection SCI rats, the nanofiber felt is revealed to recruit endogenous NSCs, induce the differentiation of NSCs into neurons while inhibit astrocytes formation and inflammation, reduces glia scar, and promotes angiogenesis, remyelination and neurological functional recovery. Overall, this innovative strategy provides a facile immune regulatory system to inhibit inflammatory response and accelerate nerve regeneration after SCI, and its targeted proteins and mechanisms are first elucidated, which holds great application promise in clinical treatment of complete SCI.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 173-194"},"PeriodicalIF":18.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11699466/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TClC effectively suppresses the growth and metastasis of NSCLC via polypharmacology TClC通过多种药理作用有效抑制非小细胞肺癌的生长和转移。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-13 DOI: 10.1016/j.bioactmat.2024.11.019

Jing Lu , Ying Zhang , Chunyan Yan , Jingwen Liu , Dan Qi , Yue Zhou , Qinwen Wang , Juechen Yang , Jing Jiang , Benhao Wu , Meiling Yang , Weiwei Zhang , Xin Zhang , Xiaoyu Shi , Yan Zhang , Kun Liu , Yongcai Liang , Chaoyang Wang , Hanyu Yang , Yuqing Gao , Guoying Zhang

{"title":"TClC effectively suppresses the growth and metastasis of NSCLC via polypharmacology","authors":"Jing Lu , Ying Zhang , Chunyan Yan , Jingwen Liu , Dan Qi , Yue Zhou , Qinwen Wang , Juechen Yang , Jing Jiang , Benhao Wu , Meiling Yang , Weiwei Zhang , Xin Zhang , Xiaoyu Shi , Yan Zhang , Kun Liu , Yongcai Liang , Chaoyang Wang , Hanyu Yang , Yuqing Gao , Guoying Zhang","doi":"10.1016/j.bioactmat.2024.11.019","DOIUrl":"10.1016/j.bioactmat.2024.11.019","url":null,"abstract":"<div><div>Despite significant advances in targeted therapies and immunotherapies, non-small cell lung cancer (NSCLC) continues to present a global health challenge, with a modest five-year survival rate of 28 %, largely due to the emergence of treatment-resistant and metastatic tumors. In response, we synthesized a novel bioactive compound, ethyl 6-chlorocoumarin-3-carboxylyl L-theanine (TClC), which significantly inhibited NSCLC growth, epithelial mesenchymal transition (EMT), migration, and invasion <em>in vitro</em> and tumor growth and metastasis <em>in vivo</em> without inducing toxicity. TClC disrupts autocrine loops that promote tumor progression, particularly in stem-like CD133-positive NSCLC (CD133+ LC) cells, which are pivotal in tumor metastasis. Through targeted molecular assays, we identified direct binding targets of TClC, including Akt, NF-κB, β-catenin, EZH2, and PD-L1. This interaction not only suppresses the expression of oncogenic factors and cancer stem cell markers but also downregulates the expression of a multidrug resistance transporter, underscoring the compound's polypharmacological potential. These results position TClC as a promising candidate for NSCLC treatment, signaling a new era in the development of cancer therapies that directly target multiple critical cancer pathways.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"45 ","pages":"Pages 567-583"},"PeriodicalIF":18.0,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11700266/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomaterial scaffold stiffness influences the foreign body reaction, tissue stiffness, angiogenesis and neuroregeneration in spinal cord injury 生物材料支架刚度影响脊髓损伤后的异物反应、组织刚度、血管生成和神经再生。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-12 DOI: 10.1016/j.bioactmat.2024.12.006

Yifeng Zheng , Maximilian Nützl , Thomas Schackel , Jing Chen , Norbert Weidner , Rainer Müller , Radhika Puttagunta

{"title":"Biomaterial scaffold stiffness influences the foreign body reaction, tissue stiffness, angiogenesis and neuroregeneration in spinal cord injury","authors":"Yifeng Zheng , Maximilian Nützl , Thomas Schackel , Jing Chen , Norbert Weidner , Rainer Müller , Radhika Puttagunta","doi":"10.1016/j.bioactmat.2024.12.006","DOIUrl":"10.1016/j.bioactmat.2024.12.006","url":null,"abstract":"<div><div>Biomaterial scaffold engineering presents great potential in promoting axonal regrowth after spinal cord injury (SCI), yet persistent challenges remain, including the surrounding host foreign body reaction and improper host-implant integration. Recent advances in mechanobiology spark interest in optimizing the mechanical properties of biomaterial scaffolds to alleviate the foreign body reaction and facilitate seamless integration. The impact of scaffold stiffness on injured spinal cords has not been thoroughly investigated. Herein, we introduce stiffness-varied alginate anisotropic capillary hydrogel scaffolds implanted into adult rat C5 spinal cords post-lateral hemisection. Four weeks post-implantation, scaffolds with a stiffness approaching that of the spinal cord effectively minimize the host foreign body reaction via yes-associated protein (YAP) nuclear translocation. Concurrently, the softest scaffolds maximize cell infiltration and angiogenesis, fostering significant axonal regrowth but limiting the rostral-caudal linear growth. Furthermore, as measured by atomic force microscopy (AFM), the surrounding spinal cord softens when in contact with the stiffest scaffold while maintaining a physiological level in contact with the softest one. In conclusion, our findings underscore the pivotal role of stiffness in scaffold engineering for SCI <em>in vivo</em>, paving the way for the optimal development of efficacious biomaterial scaffolds for tissue engineering in the central nervous system.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 134-149"},"PeriodicalIF":18.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11700269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel carbon dots with dual Modulatory effects on the bone marrow and spleen as a potential therapeutic candidate for treating spinal cord injury 对骨髓和脾脏具有双重调节作用的新型碳点作为治疗脊髓损伤的潜在候选药物。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-11 DOI: 10.1016/j.bioactmat.2024.11.032

Junjin Li , Hongda Wang , Yuanquan Li , Chunzhen Wang , Haiwen Feng , Yilin Pang , Jie Ren , Chuanhao Li , Erke Gao , Dejing Zhang , Dunxu Hu , Pengtian Zhao , Han Ding , Baoyou Fan , Tao Zhang , Xiaomeng Song , Zhijian Wei , Guangzhi Ning , Yong-Qiang Li , Shiqing Feng

{"title":"Novel carbon dots with dual Modulatory effects on the bone marrow and spleen as a potential therapeutic candidate for treating spinal cord injury","authors":"Junjin Li , Hongda Wang , Yuanquan Li , Chunzhen Wang , Haiwen Feng , Yilin Pang , Jie Ren , Chuanhao Li , Erke Gao , Dejing Zhang , Dunxu Hu , Pengtian Zhao , Han Ding , Baoyou Fan , Tao Zhang , Xiaomeng Song , Zhijian Wei , Guangzhi Ning , Yong-Qiang Li , Shiqing Feng","doi":"10.1016/j.bioactmat.2024.11.032","DOIUrl":"10.1016/j.bioactmat.2024.11.032","url":null,"abstract":"<div><div>Spinal cord injury triggers leukocyte mobilization from the peripheral circulation to the injury site, exacerbating spinal cord damage. Simultaneously, bone marrow hematopoietic stem cells (HSCs) and splenic leukocytes rapidly mobilize to replenish the depleted peripheral blood leukocyte pool. However, current treatments for spinal cord injuries overlook interventions targeting peripheral immune organs and tissues, highlighting the need to develop novel drugs capable of effectively regulating peripheral immunity and treating spinal cord injuries. In this study, we designed, synthesized, and characterized novel Ejiao carbon dots (EJCDs) that inhibit myeloid cell proliferation and peripheral migration by promoting HSC self-renewal, and distinct differentiation into erythroid progenitors in vitro and in vivo. Additionally, EJCDs attenuate the immune response in the spleen, leukocytes’ reservoir, following spinal cord injury by diminishing the local infiltration of monocytes and macrophages while promoting motor function recovery. These effects are mediated through the downregulation of CCAAT enhancer binding protein-β expression in the spleen and the upregulation of FZD4 protein expression in Lin<sup>−</sup> Sca-1<sup>+</sup> c-kit<sup>+</sup> cells (LSKs) within the bone marrow. Our findings demonstrate that EJCDs effectively reduce myeloid cell infiltration post-spinal cord injury and promote neurological recovery, making them promising therapeutic candidates for treating spinal cord injuries.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"45 ","pages":"Pages 534-550"},"PeriodicalIF":18.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11696655/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening 整合熔体静电纺丝书写和微流体技术，设计用于高保真药物筛选的人类心脏微环境。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-11 DOI: 10.1016/j.bioactmat.2024.11.037

Yu-hong Wang , Ting-ting Liu , Yan-ping Guo , Shuo-ji Zhu , Zi-ming Liao , Jia-mei Song , Xi-ming Zhu , Jia-liang Liang , Moussa Ide Nasser , Nan-bo Liu , De-hua Chang , Ping Zhu , Bin Yao

{"title":"Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening","authors":"Yu-hong Wang , Ting-ting Liu , Yan-ping Guo , Shuo-ji Zhu , Zi-ming Liao , Jia-mei Song , Xi-ming Zhu , Jia-liang Liang , Moussa Ide Nasser , Nan-bo Liu , De-hua Chang , Ping Zhu , Bin Yao","doi":"10.1016/j.bioactmat.2024.11.037","DOIUrl":"10.1016/j.bioactmat.2024.11.037","url":null,"abstract":"<div><div>The preclinical evaluation of drug-induced cardiotoxicity is critical for developing novel drug, helping to avoid drug wastage and post-marketing withdrawal. Although human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and the engineered heart organoid have been used for drug screening and mimicking disease models, they are always limited by the immaturity and lack of functionality of the cardiomyocytes. In this study, we constructed a Cardiomyocytes-on-a-Chip (CoC) that combines micro-grooves (MGs) and circulating mechanical stimulation to recapitulate the well-organized structure and stable beating of myocardial tissue. The phenotypic changes and maturation of CMs cultured on the CoC have been verified and can be used for the evaluation of cardiotoxicity and cardioprotective drug responses. Taken together, these results highlight the ability of our myocardial microarray platform to accurately reflect clinical behaviour, underscoring its potential as a powerful pre-clinical tool for assessing drug response and toxicity.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"45 ","pages":"Pages 551-566"},"PeriodicalIF":18.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11696762/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Copper–luteolin nanocomplexes for Mediating multifaceted regulation of oxidative stress, intestinal barrier, and gut microbiota in inflammatory bowel disease 铜-木犀草素纳米复合物在炎症性肠病中介导氧化应激、肠屏障和肠道微生物群的多方面调节。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-11 DOI: 10.1016/j.bioactmat.2024.12.004

Wanyue Fu , Zhongshi Huang , Weiqi Li , Lingling Xu , Miaomiao Yang , Yan Ma , Hanghang Liu , Haisheng Qian , Wanni Wang

{"title":"Copper–luteolin nanocomplexes for Mediating multifaceted regulation of oxidative stress, intestinal barrier, and gut microbiota in inflammatory bowel disease","authors":"Wanyue Fu , Zhongshi Huang , Weiqi Li , Lingling Xu , Miaomiao Yang , Yan Ma , Hanghang Liu , Haisheng Qian , Wanni Wang","doi":"10.1016/j.bioactmat.2024.12.004","DOIUrl":"10.1016/j.bioactmat.2024.12.004","url":null,"abstract":"<div><div>Oxidative stress, dysbiosis, and immune dysregulation have been confirmed to play pivotal roles in the complex pathogenesis of inflammatory bowel disease (IBD). Herein, we design copper ion–luteolin nanocomplexes (CuL NCs) through a metal–polyphenol coordination strategy, which plays a multifaceted role in the amelioration of IBD. The fabricated CuL NCs function as therapeutic agents with exceptional antioxidant and anti-inflammatory capabilities because of their great stability and capacity to scavenge reactive oxygen species (ROS). It can effectively modulate the inflammatory microenvironment including facilitating the efficient reduction of pro-inflammatory cytokine levels, protecting intestinal epithelial cells, promoting mucosal barrier repair and regulating intestinal microbiota. In addition, CuL NCs have been found to enhance cellular antioxidant and anti-inflammatory capacities by regulating the nuclear factor erythroid 2–related factor 2/heme oxygenase-1 (Nrf2/HO-1) oxidative stress pathway and nuclear factor kappa B (NF-κB) signaling pathway, respectively. Notably, CuL NCs demonstrate significant prophylactic and therapeutic efficacy in mouse models with typical IBD, including ulcerative colitis (UC) and Crohn's disease (CD). This study provides a new approach for building multifaceted therapeutic platforms for natural products to treat IBD.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 118-133"},"PeriodicalIF":18.0,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy 植物源性细胞外囊泡的脂质组学分析指导潜在的抗癌治疗。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-10 DOI: 10.1016/j.bioactmat.2024.12.001

Fei Wang , Lanya Li , Junyao Deng , Jiacong Ai , Shushan Mo , Dandan Ding , Yingxian Xiao , Shiqi Hu , Dashuai Zhu , Qishan Li , Yan Zeng , Zhitong Chen , Ke Cheng , Zhenhua Li

{"title":"Lipidomic analysis of plant-derived extracellular vesicles for guidance of potential anti-cancer therapy","authors":"Fei Wang , Lanya Li , Junyao Deng , Jiacong Ai , Shushan Mo , Dandan Ding , Yingxian Xiao , Shiqi Hu , Dashuai Zhu , Qishan Li , Yan Zeng , Zhitong Chen , Ke Cheng , Zhenhua Li","doi":"10.1016/j.bioactmat.2024.12.001","DOIUrl":"10.1016/j.bioactmat.2024.12.001","url":null,"abstract":"<div><div>Plant-derived extracellular vesicles (PEVs) have been regarded as a superior source for nanomedicine and drug delivery systems. Nevertheless, their clinical translation is hindered by the lack of clarity and even contradiction in their biomedical applications. Herein, we conducted a comprehensive compositional analysis of four commonly used PEVs to fully understand their functional lipid contents and assess their potential therapeutic applications. The lipidomic analysis revealed the presence of cytotoxic gingerols and shogaols in ginger-derived EVs (GEVs). Subsequent <em>in vitro</em> and <em>in vivo</em> investigations substantiated the remarkable tumor cell inhibitory and tumor growth suppression efficacy of GEVs. The transcriptomic analysis indicated that GEVs regulate the cell cycle and p53 signaling pathways, thereby inducing cancer cell apoptosis. The supplementary proteomic analysis suggested the potential protein markers in PEV research. These findings highlight the value of multi-omics analyses in elucidating the potential therapeutic effects of PEVs and in advancing the development of PEV-based therapies.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 82-96"},"PeriodicalIF":18.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11683192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A deformable SIS/HA composite hydrogel coaxial scaffold promotes alveolar bone regeneration after tooth extraction 可变形SIS/HA复合水凝胶同轴支架促进拔牙后牙槽骨再生。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-10 DOI: 10.1016/j.bioactmat.2024.12.008

Shiqing Ma , Yumeng Li , Shiyu Yao , Yucheng Shang , Rui Li , Lijuan Ling , Wei Fu , Pengfei Wei , Bo Zhao , Xuesong Zhang , Jiayin Deng

{"title":"A deformable SIS/HA composite hydrogel coaxial scaffold promotes alveolar bone regeneration after tooth extraction","authors":"Shiqing Ma , Yumeng Li , Shiyu Yao , Yucheng Shang , Rui Li , Lijuan Ling , Wei Fu , Pengfei Wei , Bo Zhao , Xuesong Zhang , Jiayin Deng","doi":"10.1016/j.bioactmat.2024.12.008","DOIUrl":"10.1016/j.bioactmat.2024.12.008","url":null,"abstract":"<div><div>After tooth extraction, alveolar bone absorbs unevenly, leading to soft tissue collapse, which hinders full regeneration. Bone loss makes it harder to do dental implants and repairs. Inspired by the biological architecture of bone, a deformable SIS/HA (Small intestinal submucosa/Hydroxyapatite) composite hydrogel coaxial scaffold was designed to maintain bone volume in the socket. The SIS/HA scaffold containing GL13K as the outer layer, mimicking compact bone, while SIS hydrogel loaded with bone marrow mesenchymal stem cells-derived exosomes (BMSCs-Exos) was utilized as the inner core of the scaffolds, which are like soft tissue in the skeleton. This coaxial scaffold exhibited a modulus of elasticity of 0.82 MPa, enabling it to adaptively fill extraction sockets and maintain an osteogenic space. Concurrently, the inner layer of this composite scaffold, enriched with BMSCs-Exos, promoted the proliferation and migration of human umbilical vein endothelial cells (HUVECs) and BMSCs into the scaffold interior (≈3-fold to the control), up-regulated the expression of genes related to osteogenesis (BMP2, ALP, RUNX2, and OPN) and angiogenesis (HIF-1α and VEGF). This induced new blood vessels and bone growth within the scaffold, addressing the issue of low bone formation rates at the center of defects. GL13K was released by approximately 40.87 ± 4.37 % within the first three days, exerting a localized antibacterial effect and further promoting vascularization and new bone formation in peripheral regions. This design aims to achieve an all-around and efficient bone restoration effect in the extraction socket using coaxial scaffolds through a dual internal and external mechanism.</div></div>","PeriodicalId":8762,"journal":{"name":"Bioactive Materials","volume":"46 ","pages":"Pages 97-117"},"PeriodicalIF":18.0,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11697370/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142930626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing bone regeneration through 3D printed biphasic calcium phosphate scaffolds featuring graded pore sizes 通过具有分级孔径的 3D 打印双相磷酸钙支架促进骨再生。

IF 18 1区医学

Bioactive Materials Pub Date : 2024-12-09 DOI: 10.1016/j.bioactmat.2024.11.024

Yue Wang , Yang Liu , Shangsi Chen , Ming-Fung Francis Siu , Chao Liu , Jiaming Bai , Min Wang

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