Cryopreservable, scalable and ready-to-use cell-laden patches for diabetic ulcer treatment

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2025-04-26 DOI:10.1016/j.bioactmat.2025.04.024

Bangrui Yu , Lanlan Peng , Wenjun Dang , Ying Fu , Zhijie Li , Jinteng Feng , Heng Zhao , Tian Wang , Feng Xu , Martin L. Yarmush , Haishui Huang

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引用次数: 0

Abstract

Stem cell-laden hydrogel patches are promising candidates to treat chronic ulcers due to cells’ long-lasting and dynamic responses to wound microenvironment. However, their clinical translations are prohibited by the cryopreservation difficulty due to their weak mechanical strength and slow biotransport capability, and by the morphological mismatch between clinical ulcers and pre-fabricated patches. Here we report a stem cell-laden alginate-dopamine hydrogel patch that can be readily cryopreserved, processed, and scaled toward clinical usages. This cell-hydrogel patch not only maintains cell viability and structure integrity during cryopreservation, but also can be directly utilized without centrifugation or incubation post cryopreservation. In addition, this tissue-adhesive hydrogel patch enables close wound contact and fast cellular response, and its scalable and flexible structure enables assembly for large or irregularly shaped ulcers. Therefore, it accelerates ulcer healing and reduces scar formation via continuous, versatile, self-adjusting paracrine of imbedded, cryopreserved stem cells. These findings highlight its potential for scalable clinical applications in chronic wound management and pave the way for broader adoption of ready-to-use regenerative therapies.

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用于糖尿病溃疡治疗的可低温保存、可扩展和随时使用的细胞负载贴片

由于细胞对伤口微环境的持久和动态反应，干细胞负载的水凝胶贴片是治疗慢性溃疡的有希望的候选者。然而，由于其机械强度弱，生物运输能力慢，低温保存困难，以及临床溃疡与预制贴片的形态不匹配，阻碍了其临床应用。在这里，我们报道了一种满载干细胞的藻酸盐-多巴胺水凝胶贴片，它可以很容易地冷冻保存，加工，并按比例用于临床应用。这种细胞-水凝胶贴片不仅在冷冻保存过程中保持细胞活力和结构完整性，而且在冷冻保存后无需离心或孵育即可直接利用。此外，这种组织黏附的水凝胶贴片可以使伤口紧密接触和快速的细胞反应，其可扩展和灵活的结构可以组装大型或不规则形状的溃疡。因此，它加速溃疡愈合和减少疤痕形成通过连续的，多用途的，自我调节的旁分泌嵌入，冷冻保存的干细胞。这些发现突出了其在慢性伤口管理中可扩展临床应用的潜力，并为更广泛地采用即用型再生疗法铺平了道路。

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.