Bacteriophage最新文献_第8页

Structural characterization of T7 tail machinery reveals a conserved tubular structure among other Podoviridae family members and suggests a common mechanism for DNA delivery T7尾部机械的结构特征揭示了Podoviridae家族成员中保守的管状结构，并提示了DNA传递的共同机制

Bacteriophage Pub Date : 2013-10-01 DOI: 10.4161/bact.27011

A. Cuervo, M. Chagoyen, Mar Pulido-Cid, A. Camacho, J. Carrascosa

引用次数: 5

Life in Science: Björn H Lindqvist. 科学生活:Björn H Lindqvist。

Bacteriophage Pub Date : 2013-10-01 DOI: 10.4161/bact.26673

Björn H Lindqvist

引用次数: 0

Bacteriophage K for reduction of Staphylococcus aureusbiofilm on central venous catheter material. 噬菌体K用于减少中心静脉导管材料上的金黄色葡萄球菌生物膜。

Bacteriophage Pub Date : 2013-10-01 Epub Date: 2013-10-24 DOI: 10.4161/bact.26825

Matthew P Lungren, Diana Christensen, Ravi Kankotia, Irene Falk, Ben E Paxton, Charles Y Kim

{"title":"Bacteriophage K for reduction of Staphylococcus aureusbiofilm on central venous catheter material.","authors":"Matthew P Lungren, Diana Christensen, Ravi Kankotia, Irene Falk, Ben E Paxton, Charles Y Kim","doi":"10.4161/bact.26825","DOIUrl":"https://doi.org/10.4161/bact.26825","url":null,"abstract":"The purpose of this project was to determine whether bacteriophage can reduce bacterial colonization and biofilm formation on central venous catheter material. Twenty silicone discs were inoculated for 24 h with broth culture of Methicillin sensitive staphylococcus aureus (0.5 McFarland standard). The inoculate was aspirated and discs placed into two equal groups for 24 h: (1) untreated controls; (2) bacteriophage treatment (staphylococcal bacteriophage K, propagated titer > 108). At the completion of the experiment discs were processed for quantitative culture. Statistical testing was performed using the rank sum test. Mean colony forming units (CFU) were significantly decreased in experimental compared with controls (control 6.3 × 105 CFU, experimental 6.7 × 101, P ≤ 0.0001). Application of bacteriophage to biofilm infected central venous catheter material significantly reduced bacterial colonization and biofilm presence. Our data suggests that bacteriophage treatment may be a feasible strategy for addressing central venous catheter staph aureus biofilm infections.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 4","pages":"e26825"},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.26825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31893989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Cyanophages infecting Anabaena circinalis and Anabaena cylindrica in a tropical reservoir 热带水库中感染环状水蛭和圆柱水蛭的噬藻体

Bacteriophage Pub Date : 2013-07-11 DOI: 10.4161/bact.25571

B. Yeo, K. Gin

引用次数: 9

Tale of the unseen phage 看不见的噬菌体的故事

Bacteriophage Pub Date : 2013-07-11 DOI: 10.4161/bact.25985

J. Mahony, S. Ainsworth, D. van Sinderen

引用次数: 1

Bacteriophages lytic for Salmonella rapidly reduce Salmonella contamination on glass and stainless steel surfaces. 沙门氏菌噬菌体可迅速减少玻璃和不锈钢表面的沙门氏菌污染。

Bacteriophage Pub Date : 2013-07-01 Epub Date: 2013-07-26 DOI: 10.4161/bact.25697

Joelle Woolston, Adam R Parks, Tamar Abuladze, Bradley Anderson, Manrong Li, Chandi Carter, Leigh Farris Hanna, Serena Heyse, Duane Charbonneau, Alexander Sulakvelidze

{"title":"Bacteriophages lytic for Salmonella rapidly reduce Salmonella contamination on glass and stainless steel surfaces.","authors":"Joelle Woolston, Adam R Parks, Tamar Abuladze, Bradley Anderson, Manrong Li, Chandi Carter, Leigh Farris Hanna, Serena Heyse, Duane Charbonneau, Alexander Sulakvelidze","doi":"10.4161/bact.25697","DOIUrl":"https://doi.org/10.4161/bact.25697","url":null,"abstract":"A cocktail of six lytic bacteriophages, SalmoFresh™, significantly (p < 0.05) reduced the number of surface-applied Salmonella Kentucky and Brandenburg from stainless steel and glass surfaces by > 99% (2.1–4.3 log). Both strains were susceptible to SalmoFresh™ in the spot-test assay. Conversely, SalmoFresh™ was unable to reduce surface contamination with a Salmonella Paratyphi B strain that was not susceptible to the phage cocktail in the spot-test assay. However, by replacing two SalmoFresh™ component phages with two new phages capable of lysing the Paratyphi B strain in the spot-test assay, the target range of the cocktail was shifted to include the Salmonella Paratyphi B strain. The modified cocktail, SalmoLyse™, was able to significantly (p < 0.05) reduce surface contamination of the Paratyphi B strain by > 99% (2.1–4.1 log). The data show that both phage cocktails were effective in significantly reducing the levels of Salmonella on hard surfaces, provided the contaminating strains were susceptible in the spot-test (i.e., spot-test susceptibility was indicative of efficacy in subsequent surface decontamination studies). The data also support the concept that phage preparations can be customized to meet the desired antibacterial application.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 3","pages":"e25697"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.25697","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31862928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Life in Science: Armin Dale Kaiser. 《科学生活》:阿明·戴尔·凯泽。

Bacteriophage Pub Date : 2013-07-01 Epub Date: 2013-06-28 DOI: 10.4161/bact.25589

Armin Dale Kaiser

引用次数: 0

Innate and acquired bacteriophage-mediated immunity. 先天和获得性噬菌体介导免疫。

Bacteriophage Pub Date : 2013-07-01 Epub Date: 2013-07-29 DOI: 10.4161/bact.25857

Jeremy J Barr, Merry Youle, Forest Rohwer

引用次数: 66

Properties and mutation studies of a bacteriophage-derived chimeric recombinant staphylolytic protein P128: Comparison to recombinant lysostaphin. 噬菌体衍生的嵌合重组葡萄球菌溶解蛋白P128的性质和突变研究:与重组溶葡萄球菌蛋白的比较。

Bacteriophage Pub Date : 2013-07-01 Epub Date: 2013-10-02 DOI: 10.4161/bact.26564

Sanjeev Rajagopalan Saravanan, Vivek Daniel Paul, Shilpa George, Sudarson Sundarrajan, Nirmal Kumar, Madhavi Hebbur, Naveen Kumar, Ananda Veena, Uma Maheshwari, Chemira Biddappa Appaiah, Muralidharan Chidambaran, Anuradha Gopal Bhat, Sukumar Hariharan, Sriram Padmanabhan

{"title":"Properties and mutation studies of a bacteriophage-derived chimeric recombinant staphylolytic protein P128: Comparison to recombinant lysostaphin.","authors":"Sanjeev Rajagopalan Saravanan, Vivek Daniel Paul, Shilpa George, Sudarson Sundarrajan, Nirmal Kumar, Madhavi Hebbur, Naveen Kumar, Ananda Veena, Uma Maheshwari, Chemira Biddappa Appaiah, Muralidharan Chidambaran, Anuradha Gopal Bhat, Sukumar Hariharan, Sriram Padmanabhan","doi":"10.4161/bact.26564","DOIUrl":"https://doi.org/10.4161/bact.26564","url":null,"abstract":"P128 is a chimeric anti-staphylococcal protein having a catalytic domain from a Staphylococcus bacteriophage K tail associated structural protein and a cell wall targeting domain from the Staphylococcus bacteriocin-lysostaphin. In this study, we disclose additional properties of P128 and compared the same with lysostaphin. While lysostaphin was found to get inactivated by heat and was inactive on its parent strain S. simulans biovar staphylolyticus, P128 was thermostable and was lytic towards S. simulans biovar staphylolyticus demonstrating a difference in their mechanism of action. Selected mutation studies of the catalytic domain of P128 showed that arginine and cysteine, at 40th and 76th positions respectively, are critical for the staphylolytic activity of P128, although these amino acids are not conserved residues. In comparison to native P128, only the R40S mutant (P301) was catalytically active on zymogram gel and had a similar secondary structure, as assessed by circular dichroism analysis and in silico modeling with similar cell binding properties. Mutation of the arginine residue at 40th position of the P128 molecule caused dramatic reduction in the Vmax (∆OD600 [mg/min]) value (nearly 270 fold) and the recombinant lysostaphin also showed lesser Vmax value (nearly 1.5 fold) in comparison to the unmodified P128 protein. The kinetic parameters such as apparent Km (KmAPP) and apparent Kcat (KcatAPP) of the native P128 protein also showed significant differences in comparison to the values observed for P301 and lysostaphin.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 3","pages":"e26564"},"PeriodicalIF":0.0,"publicationDate":"2013-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.26564","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31881535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Listeria phages: Genomes, evolution, and application. 李斯特菌噬菌体：基因组、进化和应用。

Bacteriophage Pub Date : 2013-07-01 Epub Date: 2013-10-24 DOI: 10.4161/bact.26861

Jochen Klumpp, Martin J Loessner

引用次数: 0