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The XXIIIrd Phage/Virus Assembly Meeting. 第二十三届噬菌体/病毒大会。
Bacteriophage Pub Date : 2014-01-01 DOI: 10.4161/bact.27272
Philip Serwer
{"title":"The XXIIIrd Phage/Virus Assembly Meeting.","authors":"Philip Serwer","doi":"10.4161/bact.27272","DOIUrl":"https://doi.org/10.4161/bact.27272","url":null,"abstract":"<p><p>The XXIIIrd Phage/Virus Assembly (PVA) meeting returned to its birthplace in Lake Arrowhead, CA on September 8-13, 2013 (Fig. 1). The original meeting occurred in 1968, organized by Bob Edgar (Caltech, Pasadena, CA USA), Fred Eiserling (University of California, Los Angeles, Los Angeles, CA USA) and Bill Wood (Caltech, Pasadena, CA USA). The organizers of the 2013 meeting were Bill Gelbart (University of California, Los Angeles, Los Angeles, CA USA) and Jack Johnson (Scripps Research Institute, La Jolla, CA USA). This meeting specializes in an egalitarian format. Students are distinguished from senior faculty primarily by the signs of age. With the exception of historically based introductory talks, all talks were allotted the same time and freedom. This tradition began when the meeting was phage-only and has been continued now that all viruses are included. Many were the animated conversations about basic questions. New and international participants were present, a sign that the field has significant attraction, as it should, based on details below. The meeting was also characterized by a sense of humor and generally good times, a chance to both enjoy the science and forget the funding malaise to which many participants are exposed. I will present some of the meeting content, without attempting to be comprehensive.</p>","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.27272","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32093590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Bacteriophage as instructional organisms in introductory biology labs. 噬菌体在生物学导论实验室中的指导作用。
Bacteriophage Pub Date : 2014-01-01 DOI: 10.4161/bact.27336
Paul Hyman
{"title":"Bacteriophage as instructional organisms in introductory biology labs.","authors":"Paul Hyman","doi":"10.4161/bact.27336","DOIUrl":"https://doi.org/10.4161/bact.27336","url":null,"abstract":"<p><p>Designing lab exercises for introductory biology classes requires balancing the need for students to obtain results with a desire to provide unpredictable outcomes to better approximate actual research. Bacteriophage are particularly well suited for this as many species are well-understood but, with their hosts, represent a relatively complex interacting system. I have designed a seven week series of lab exercises that allow students to select bacteriophage resistant mutant hosts, isolate and sequence the corresponding receptor gene to identify the specific bacterial mutation from a large number of potential mutations. I also examined the possibility of collecting useful mutant strains for other studies. After two semesters, the lab series is working well with over 90% of students successfully isolating mutant bacteria and about half identifying the specific mutation. Here I discuss the advantages of using bacteriophage in an introductory class, the specific labs in this series and future plans.</p>","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.27336","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32076353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Molecular architecture of tailed double-stranded DNA phages. 尾部双链DNA噬菌体的分子结构。
Bacteriophage Pub Date : 2014-01-01 Epub Date: 2014-02-21 DOI: 10.4161/bact.28281
Andrei Fokine, Michael G Rossmann
{"title":"Molecular architecture of tailed double-stranded DNA phages.","authors":"Andrei Fokine,&nbsp;Michael G Rossmann","doi":"10.4161/bact.28281","DOIUrl":"https://doi.org/10.4161/bact.28281","url":null,"abstract":"<p><p>The tailed double-stranded DNA bacteriophages, or <i>Caudovirales</i>, constitute ~96% of all the known phages. Although these phages come in a great variety of sizes and morphology, their virions are mainly constructed of similar molecular building blocks via similar assembly pathways. Here we review the structure of tailed double-stranded DNA bacteriophages at a molecular level, emphasizing the structural similarity and common evolutionary origin of proteins that constitute these virions.</p>","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.28281","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40299382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 189
Identification of the ssDNA-binding protein of bacteriophage T5: Implications for T5 replication. 噬菌体T5的ssdna结合蛋白的鉴定:对T5复制的意义。
Bacteriophage Pub Date : 2013-10-01 Epub Date: 2013-12-09 DOI: 10.4161/bact.27304
Sebastiaan Werten
{"title":"Identification of the ssDNA-binding protein of bacteriophage T5: Implications for T5 replication.","authors":"Sebastiaan Werten","doi":"10.4161/bact.27304","DOIUrl":"https://doi.org/10.4161/bact.27304","url":null,"abstract":"<p><p>In a recent study, we identified and characterized the long-elusive replicative single-stranded DNA-binding protein of bacteriophage T5, which we showed is related to the eukaryotic transcription coactivator PC4. Here, we provide an extended discussion of these data, report several additional observations and consider implications for the recombination-dependent replication mechanism of the T5 genus, which is still poorly understood.</p>","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.27304","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32078279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Identification and characterization of ϕH111-1: A novel myovirus with broad activity against clinical isolates of Burkholderia cenocepacia. 一种新型肌病毒的鉴定和特性:对临床分离的结核杆菌具有广泛活性。
Bacteriophage Pub Date : 2013-10-01 DOI: 10.4161/bact.26649
Karlene H Lynch, Yongjie Liang, Leo Eberl, David S Wishart, Jonathan J Dennis
{"title":"Identification and characterization of ϕH111-1: A novel myovirus with broad activity against clinical isolates of <i>Burkholderia cenocepacia.</i>","authors":"Karlene H Lynch,&nbsp;Yongjie Liang,&nbsp;Leo Eberl,&nbsp;David S Wishart,&nbsp;Jonathan J Dennis","doi":"10.4161/bact.26649","DOIUrl":"https://doi.org/10.4161/bact.26649","url":null,"abstract":"<p><p>Characterization of prophages in sequenced bacterial genomes is important for virulence assessment, evolutionary analysis, and phage application development. The objective of this study was to identify complete, inducible prophages in the cystic fibrosis (CF) clinical isolate <i>Burkholderia cenocepacia</i> H111. Using the prophage-finding program PHAge Search Tool (PHAST), we identified three putative intact prophages in the H111 sequence. Virions were readily isolated from H111 culture supernatants following extended incubation. Using shotgun cloning and sequencing, one of these virions (designated ϕH111-1 [vB_BceM_ϕH111-1]) was identified as the infective particle of a PHAST-detected intact prophage. ϕH111-1 has an extremely broad host range with respect to <i>B. cenocepacia</i> strains and is predicted to use lipopolysaccharide (LPS) as a receptor. Bioinformatics analysis indicates that the prophage is 42,972 base pairs in length, encodes 54 proteins, and shows relatedness to the virion morphogenesis modules of <i>Aca</i>ML1 and \"Vhmllikevirus\" myoviruses. As ϕH111-1 is active against a broad panel of clinical strains and encodes no putative virulence factors, it may be therapeutically effective for <i>Burkholderia</i> infections.</p>","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.26649","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31893988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Structural characterization of T7 tail machinery reveals a conserved tubular structure among other Podoviridae family members and suggests a common mechanism for DNA delivery T7尾部机械的结构特征揭示了Podoviridae家族成员中保守的管状结构，并提示了DNA传递的共同机制
Bacteriophage Pub Date : 2013-10-01 DOI: 10.4161/bact.27011
A. Cuervo, M. Chagoyen, Mar Pulido-Cid, A. Camacho, J. Carrascosa
{"title":"Structural characterization of T7 tail machinery reveals a conserved tubular structure among other Podoviridae family members and suggests a common mechanism for DNA delivery","authors":"A. Cuervo, M. Chagoyen, Mar Pulido-Cid, A. Camacho, J. Carrascosa","doi":"10.4161/bact.27011","DOIUrl":"https://doi.org/10.4161/bact.27011","url":null,"abstract":"Bacteriophage tail complexes play an essential role in host recognition and DNA delivery during virus infection. These molecular machines are composed of a tubular structure surrounded by fibers, with a central channel that acts as a conduit for DNA ejection. The T7 tail complex is formed by four proteins: connector (gp8), gatekeeper (gp11), nozzle (gp12), and fibers (gp17). Previous biochemical and structural studies allowed definition of the stoichiometry and order of assembly of these proteins. Here we compared the tail complex from other Podoviridae phages that infect bacteria with Gram− type envelopes (K1E, P-SSP7, and ε15), and found strong similarities with the T7 nozzle; this was supported by sequence alignment and secondary structure prediction studies. These similarities were not observed in the new reconstruction of protein p9 presented here, which builds the hexameric nozzle of ϕ29, a virus that infects Gram+ bacteria. The results suggest that the Podoviridae nozzle has evolved to adapt to membrane composition of the infected host.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89481760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Life in Science: Björn H Lindqvist. 科学生活:Björn H Lindqvist。
Bacteriophage Pub Date : 2013-10-01 DOI: 10.4161/bact.26673
Björn H Lindqvist
{"title":"Life in Science: Björn H Lindqvist.","authors":"Björn H Lindqvist","doi":"10.4161/bact.26673","DOIUrl":"https://doi.org/10.4161/bact.26673","url":null,"abstract":"","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.26673","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31881536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bacteriophage K for reduction of Staphylococcus aureusbiofilm on central venous catheter material. 噬菌体K用于减少中心静脉导管材料上的金黄色葡萄球菌生物膜。
Bacteriophage Pub Date : 2013-10-01 Epub Date: 2013-10-24 DOI: 10.4161/bact.26825
Matthew P Lungren, Diana Christensen, Ravi Kankotia, Irene Falk, Ben E Paxton, Charles Y Kim
{"title":"Bacteriophage K for reduction of <i>Staphylococcus aureus</i>biofilm on central venous catheter material.","authors":"Matthew P Lungren,&nbsp;Diana Christensen,&nbsp;Ravi Kankotia,&nbsp;Irene Falk,&nbsp;Ben E Paxton,&nbsp;Charles Y Kim","doi":"10.4161/bact.26825","DOIUrl":"https://doi.org/10.4161/bact.26825","url":null,"abstract":"<p><p>The purpose of this project was to determine whether bacteriophage can reduce bacterial colonization and biofilm formation on central venous catheter material. Twenty silicone discs were inoculated for 24 h with broth culture of Methicillin sensitive <i>staphylococcus aureus</i> (0.5 McFarland standard). The inoculate was aspirated and discs placed into two equal groups for 24 h: (1) untreated controls; (2) bacteriophage treatment (<i>staphylococcal</i> bacteriophage K, propagated titer > 10<sup>8</sup>). At the completion of the experiment discs were processed for quantitative culture. Statistical testing was performed using the rank sum test. Mean colony forming units (CFU) were significantly decreased in experimental compared with controls (control 6.3 × 10<sup>5</sup> CFU, experimental 6.7 × 10<sup>1</sup>, <i>P</i> ≤ 0.0001). Application of bacteriophage to biofilm infected central venous catheter material significantly reduced bacterial colonization and biofilm presence. Our data suggests that bacteriophage treatment may be a feasible strategy for addressing central venous catheter staph aureus biofilm infections.</p>","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.26825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31893989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 27
Cyanophages infecting Anabaena circinalis and Anabaena cylindrica in a tropical reservoir 热带水库中感染环状水蛭和圆柱水蛭的噬藻体
Bacteriophage Pub Date : 2013-07-11 DOI: 10.4161/bact.25571
B. Yeo, K. Gin
{"title":"Cyanophages infecting Anabaena circinalis and Anabaena cylindrica in a tropical reservoir","authors":"B. Yeo, K. Gin","doi":"10.4161/bact.25571","DOIUrl":"https://doi.org/10.4161/bact.25571","url":null,"abstract":"A total of six lytic cyanophages have been isolated from Kranji Reservoir in Singapore. Two cyanophages infected a toxic species of cyanobacterium, A. circinalis, while four cyanophages infected the cyanobacterium A. cylindrica. To obtain the cyanophages that infect A. circinalis and A. cylindrica, two isolation methods were established. Cyanophages infecting A. circinalis were successfully isolated with well assay, and cyaophages infecting A. cylindrica were successfully isolated with double layer plaque assay. These isolation methods resulted in a rapid and significant lytic effect. A. circinalis cyanophage isolates are potential biocontrol of A. circinalis, due to the ability of cyanophage isolates in suppressing the growth of A. circinalis effectively. During the isolation process, we observed that A. cylindrica experienced changes in aggregation and algal mat formation when inoculated with the cyanophage isolates.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86202315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 9
Tale of the unseen phage 看不见的噬菌体的故事
Bacteriophage Pub Date : 2013-07-11 DOI: 10.4161/bact.25985
J. Mahony, S. Ainsworth, D. van Sinderen
{"title":"Tale of the unseen phage","authors":"J. Mahony, S. Ainsworth, D. van Sinderen","doi":"10.4161/bact.25985","DOIUrl":"https://doi.org/10.4161/bact.25985","url":null,"abstract":"Lactococcal phages are among the best characterized phages of Gram-positive bacteria and as such represent excellent models to promote our understanding of phage-host interactions. In recent years, microarray technology has been employed to define the transcriptional profile of phages during infection, while simultaneously uncovering the response of the host to the infection event. The responses to infection by a lytic and a temperate phage of Lactococcus lactis were analyzed, combined with an assessment of the host response to lysogenisation with the temperate phage, Tuc2009. Here, we discuss the lessons learned from such transcriptional profiling studies, focusing on studies relating to phages of Lactococcus lactis, and how this knowledge may be applied to future studies.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90219115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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