Bacteriophages lytic for Salmonella rapidly reduce Salmonella contamination on glass and stainless steel surfaces.

Bacteriophage Pub Date : 2013-07-01 Epub Date: 2013-07-26 DOI:10.4161/bact.25697
Joelle Woolston, Adam R Parks, Tamar Abuladze, Bradley Anderson, Manrong Li, Chandi Carter, Leigh Farris Hanna, Serena Heyse, Duane Charbonneau, Alexander Sulakvelidze
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引用次数: 1

Abstract

A cocktail of six lytic bacteriophages, SalmoFresh™, significantly (p < 0.05) reduced the number of surface-applied Salmonella Kentucky and Brandenburg from stainless steel and glass surfaces by > 99% (2.1–4.3 log). Both strains were susceptible to SalmoFresh™ in the spot-test assay. Conversely, SalmoFresh™ was unable to reduce surface contamination with a Salmonella Paratyphi B strain that was not susceptible to the phage cocktail in the spot-test assay. However, by replacing two SalmoFresh™ component phages with two new phages capable of lysing the Paratyphi B strain in the spot-test assay, the target range of the cocktail was shifted to include the Salmonella Paratyphi B strain. The modified cocktail, SalmoLyse™, was able to significantly (p < 0.05) reduce surface contamination of the Paratyphi B strain by > 99% (2.1–4.1 log). The data show that both phage cocktails were effective in significantly reducing the levels of Salmonella on hard surfaces, provided the contaminating strains were susceptible in the spot-test (i.e., spot-test susceptibility was indicative of efficacy in subsequent surface decontamination studies). The data also support the concept that phage preparations can be customized to meet the desired antibacterial application.

Abstract Image

Abstract Image

沙门氏菌噬菌体可迅速减少玻璃和不锈钢表面的沙门氏菌污染。
六种溶解噬菌体SalmoFresh™的混合物显著(p < 0.05)减少了表面应用于不锈钢和玻璃表面的肯塔基沙门氏菌和勃兰登堡沙门氏菌的数量> 99% (2.1-4.3 log)。在现场试验中,两株菌株对SalmoFresh™敏感。相反,SalmoFresh™无法减少对噬菌体混合物不敏感的副伤寒沙门氏菌B株的表面污染。然而,通过在现场试验中将两个SalmoFresh™成分噬菌体替换为两个能够裂解副伤寒沙门氏菌B株的新噬菌体,鸡尾酒的目标范围被转移到包括副伤寒沙门氏菌B株。改良后的鸡尾酒SalmoLyse™能够显著(p < 0.05)减少乙型副伤寒菌株表面污染> 99% (2.1-4.1 log)。数据表明,两种噬菌体鸡尾酒都能有效地显著降低坚硬表面上沙门氏菌的水平,前提是污染菌株在现场试验中敏感(即,在随后的表面去污研究中,现场试验的敏感性表明了有效性)。这些数据还支持噬菌体制剂可以定制以满足所需抗菌应用的概念。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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