Structural characterization of T7 tail machinery reveals a conserved tubular structure among other Podoviridae family members and suggests a common mechanism for DNA delivery

Bacteriophage Pub Date : 2013-10-01 DOI:10.4161/bact.27011
A. Cuervo, M. Chagoyen, Mar Pulido-Cid, A. Camacho, J. Carrascosa
{"title":"Structural characterization of T7 tail machinery reveals a conserved tubular structure among other Podoviridae family members and suggests a common mechanism for DNA delivery","authors":"A. Cuervo, M. Chagoyen, Mar Pulido-Cid, A. Camacho, J. Carrascosa","doi":"10.4161/bact.27011","DOIUrl":null,"url":null,"abstract":"Bacteriophage tail complexes play an essential role in host recognition and DNA delivery during virus infection. These molecular machines are composed of a tubular structure surrounded by fibers, with a central channel that acts as a conduit for DNA ejection. The T7 tail complex is formed by four proteins: connector (gp8), gatekeeper (gp11), nozzle (gp12), and fibers (gp17). Previous biochemical and structural studies allowed definition of the stoichiometry and order of assembly of these proteins. Here we compared the tail complex from other Podoviridae phages that infect bacteria with Gram− type envelopes (K1E, P-SSP7, and ε15), and found strong similarities with the T7 nozzle; this was supported by sequence alignment and secondary structure prediction studies. These similarities were not observed in the new reconstruction of protein p9 presented here, which builds the hexameric nozzle of ϕ29, a virus that infects Gram+ bacteria. The results suggest that the Podoviridae nozzle has evolved to adapt to membrane composition of the infected host.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bacteriophage","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4161/bact.27011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

Bacteriophage tail complexes play an essential role in host recognition and DNA delivery during virus infection. These molecular machines are composed of a tubular structure surrounded by fibers, with a central channel that acts as a conduit for DNA ejection. The T7 tail complex is formed by four proteins: connector (gp8), gatekeeper (gp11), nozzle (gp12), and fibers (gp17). Previous biochemical and structural studies allowed definition of the stoichiometry and order of assembly of these proteins. Here we compared the tail complex from other Podoviridae phages that infect bacteria with Gram− type envelopes (K1E, P-SSP7, and ε15), and found strong similarities with the T7 nozzle; this was supported by sequence alignment and secondary structure prediction studies. These similarities were not observed in the new reconstruction of protein p9 presented here, which builds the hexameric nozzle of ϕ29, a virus that infects Gram+ bacteria. The results suggest that the Podoviridae nozzle has evolved to adapt to membrane composition of the infected host.
T7尾部机械的结构特征揭示了Podoviridae家族成员中保守的管状结构,并提示了DNA传递的共同机制
在病毒感染过程中,噬菌体尾部复合物在宿主识别和DNA传递中起着重要作用。这些分子机器是由一个被纤维包围的管状结构组成的,有一个中央通道作为DNA喷射的管道。T7尾部复合体由四种蛋白质组成:连接器(gp8)、看门人(gp11)、喷嘴(gp12)和纤维(gp17)。先前的生化和结构研究允许对这些蛋白质的化学计量和组装顺序进行定义。本文比较了其他具有革兰氏型包膜(K1E、P-SSP7和ε15)的足病毒科噬菌体的尾部复合体,发现与T7喷嘴有很强的相似性;这得到了序列比对和二级结构预测研究的支持。在构建感染革兰氏阳性细菌的病毒- - - 29的六聚体喷嘴的蛋白质p9的新重建中没有观察到这些相似性。结果表明,足病毒科的喷嘴已经进化到适应被感染宿主的膜组成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信