Bacteriophage最新文献_第9页

Life in Science: Richard Calendar. 科学生活:Richard Calendar。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.25516

Richard Calendar

引用次数: 1

What are the limitations on the wider therapeutic use of phage? 噬菌体在更广泛的治疗用途方面有哪些限制？

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.24872

Alexandra Henein

{"title":"What are the limitations on the wider therapeutic use of phage?","authors":"Alexandra Henein","doi":"10.4161/bact.24872","DOIUrl":"10.4161/bact.24872","url":null,"abstract":"Bacterial resistance to antibiotics poses a serious health threat. Since research into new antibiotics is not progressing at the same rate as the development of bacterial resistance, widespread calls for alternatives to antibiotics have been made. Phage therapy is an ideal alternative candidate to be investigated. However the success of phage therapy may be hampered by a lack of investment support from large pharmaceutical companies, due to their narrow spectrum of activity in antibiotics, very large costs associated with clinical trials of the variety of phages needed, and regulatory requirements remaining unclear. Intellectual property is difficult to secure for therapeutic phage products for a variety of reasons, and patenting procedures vary widely between the US and the EU. Consequently, companies are more likely to invest in phage products for decontamination or veterinary use, rather than clinical use in humans. Some still raise questions as to the safety of phage therapy overall, suggesting the possibility of cytotoxicity and immunogenicity, depending on the phage preparation and route. On the other hand, with patients dying because of infections untreatable with conventional antibiotics, the question arises as to whether it is ethical not to pursue phage therapy more diligently. A paradigm shift about how phage therapy is perceived is required, as well as more rigorous proof of efficacy in the form of clinical trials of existing medicinal phage products. Phage therapy potential may be fulfilled in the meantime by allowing individual preparations to be used on a named-patient basis, with extensive monitoring and multidisciplinary team input. The National Health Service and academia have a role in carrying out clinical phage research, which would be beneficial to public health, but not necessarily financially rewarding.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 2","pages":"e24872"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/02/c2/bact-3-e24872.PMC3821673.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31862922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lytic bacteriophages: Potential interventions against enteric bacterial pathogens on produce. 噬菌体：对农产品肠道细菌病原体的潜在干预措施。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.25518

Manan Sharma

{"title":"Lytic bacteriophages: Potential interventions against enteric bacterial pathogens on produce.","authors":"Manan Sharma","doi":"10.4161/bact.25518","DOIUrl":"10.4161/bact.25518","url":null,"abstract":"Foodborne illnesses resulting from the consumption of produce commodities contaminated with enteric pathogens continue to be a significant public health issue. Lytic bacteriophages may provide an effective and natural intervention to reduce bacterial pathogens on fresh and fresh-cut produce commodities. The use of multi-phage cocktails specific for a single pathogen has been most frequently assessed on produce commodities to minimize the development of bacteriophage insensitive mutants (BIM) in target pathogen populations. Regulatory approval for the use of several lytic phage products specific for bacterial pathogens such as Escherichia coli O157:H7, Salmonella spp. and Listeria monocytogenes in foods and on food processing surfaces has been granted by various agencies in the US and other countries, possibly allowing for the more widespread use of bacteriophages in the decontamination of fresh and minimally processed produce. Research studies have shown lytic bacteriophages specific for E. coli O157:H7, Salmonella spp. and Listeria monocytogenes have been effective in reducing pathogen populations on leafy greens, sprouts and tomatoes.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 2","pages":"e25518"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/81/06/bact-3-e25518.PMC3821672.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31862925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Upcoming meetings 即将到来的会议

Bacteriophage Pub Date : 2013-04-01 DOI: 10.1111/1744-9987.12133

Alexander Sulakvelidze

引用次数: 0

Enzymatic characterization of a lysin encoded by bacteriophage EL. 噬菌体EL编码的溶解酶的酶学特性。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.25449

Diana A Tafoya, Zacariah L Hildenbrand, Nadia Herrera, Sudheer K Molugu, Vadim V Mesyanzhinov, Konstantin A Miroshnikov, Ricardo A Bernal

引用次数: 4

Novel group of podovirus infecting the marine bacterium Alteromonas macleodii. 感染海洋细菌巨形异单胞菌的新型足病毒群。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.24766

Inmaculada Garcia-Heredia, Francisco Rodriguez-Valera, Ana-Belen Martin-Cuadrado

{"title":"Novel group of podovirus infecting the marine bacterium Alteromonas macleodii.","authors":"Inmaculada Garcia-Heredia, Francisco Rodriguez-Valera, Ana-Belen Martin-Cuadrado","doi":"10.4161/bact.24766","DOIUrl":"https://doi.org/10.4161/bact.24766","url":null,"abstract":"Four novel, closely related podoviruses, which displayed lytic activity against the gamma-proteobacterium Alteromonas macleodii, have been isolated and sequenced. Alterophages AltAD45-P1 to P4 were obtained from water recovered near a fish farm in the Mediterranean Sea. Their morphology indicates that they belong to the Podoviridae. Their linear and dsDNA genomes are 100-104 kb in size, remarkably larger than any other described podovirus. The four AltAD45-phages share 99% nucleotide sequence identity over 97% of their ORFs, although an insertion was found in AltAD45-P1 and P2 and some regions were slightly more divergent. Despite the high overall sequence similarity among these four phages, the group with the insertion and the group without it, have different host ranges against the A. macleodii strains tested. The AltAD45-P1 to P4 phages have genes for DNA replication and transcription as well as structural genes, which are similar to the N4-like Podoviridae genus that is widespread in proteobacteria. However, in terms of their genomic structure, AltAD45-P1 to P4 differ from that of the N4-like phages. Some distinguishing features include the lack of a large virion encapsidated RNA polymerase gene, very well conserved among all the previously described N4-like phages, a single-stranded DNA binding protein and different tail protein genes. We conclude that the AltAD45 phages characterized in this study constitute a new genus within the Podoviridae.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 2","pages":"e24766"},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.24766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31862921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

The moonlighting function of bacteriophage P4 capsid protein, Psu, as a transcription antiterminator. 噬菌体P4衣壳蛋白(Psu)作为转录抗终结者的兼职功能。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.25657

Amitabh Ranjan, Ramanuj Banerjee, Bibhusita Pani, Udayditya Sen, Ranjan Sen

引用次数: 9

Evolution of genetic switch complexity. 遗传开关复杂性的进化。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24186

Gregory W Broussard, Graham F Hatfull

引用次数: 10

Biocontrol of Escherichia coli O157: H7 on fresh-cut leafy greens. 鲜切绿叶蔬菜O157: H7大肠杆菌的生物防治。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24620

Olcay Boyacioglu, Manan Sharma, Alexander Sulakvelidze, Ipek Goktepe

{"title":"Biocontrol of Escherichia coli O157: H7 on fresh-cut leafy greens.","authors":"Olcay Boyacioglu, Manan Sharma, Alexander Sulakvelidze, Ipek Goktepe","doi":"10.4161/bact.24620","DOIUrl":"https://doi.org/10.4161/bact.24620","url":null,"abstract":"The effect of a bacteriophage cocktail (EcoShield™) that is specific against Escherichia coli O157:H7 was evaluated against a nalidixic acid-resistant enterohemorrhagic E. coli O157:H7 RM4407 (EHEC) strain on leafy greens stored under either (1) ambient air or (2) modified atmosphere (MA; 5% O2/35% CO2/60% N2). Pieces (~2 × 2 cm2) of leafy greens (lettuce and spinach) inoculated with 4.5 log CFU/cm2 EHEC were sprayed with EcoShield™ (6.5 log PFU/cm2). Samples were stored at 4 or 10°C for up to 15 d. On spinach, the level of EHEC declined by 2.38 and 2.49 log CFU/cm2 at 4 and 10°C, respectively, 30 min after phage application (p ≤ 0.05). EcoShield™ was also effective in reducing EHEC on the surface of green leaf lettuce stored at 4°C by 2.49 and 3.28 log units in 30 min and 2 h, respectively (p ≤ 0.05). At 4°C under atmospheric air, the phage cocktail significantly (p ≤ 0.05) lowered the EHEC counts in one day by 1.19, 3.21 and 3.25 log CFU/cm2 on spinach, green leaf and romaine lettuce, respectively compared with control (no bacteriophage) treatments. When stored under MA at 4°C, phages reduced (p ≤ 0.05) EHEC populations by 2.18, 3.50 and 3.13 log CFU/cm2, on spinach, green leaf and romaine lettuce. At 10°C, EHEC reductions under atmospheric air storage were 1.99, 3.90 and 3.99 log CFU/cm2 (p ≤ 0.05), while population reductions under MA were 3.08, 3.89 and 4.34 logs on spinach, green leaf and romaine lettuce, respectively, compared with controls (p ≤ 0.05). The results of this study showed that bacteriophages were effective in reducing the levels of E. coli O157:H7 on fresh leafy produce, and that the reduction was further improved when produce was stored under the MA conditions.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":"3 1","pages":"e24620"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.24620","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31192111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Anna S. Tikhonenko: Electron microscopist extraordinary. Anna S. Tikhonenko:电子显微镜专家。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.23646

Vladimir I Popenko, Elizabeth M Kutter, Hans-W Ackermann

引用次数: 4