Bacteriophage最新文献_第10页

Novel group of podovirus infecting the marine bacterium Alteromonas macleodii. 感染海洋细菌巨形异单胞菌的新型足病毒群。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.24766

Inmaculada Garcia-Heredia, Francisco Rodriguez-Valera, Ana-Belen Martin-Cuadrado

{"title":"Novel group of podovirus infecting the marine bacterium Alteromonas macleodii.","authors":"Inmaculada Garcia-Heredia, Francisco Rodriguez-Valera, Ana-Belen Martin-Cuadrado","doi":"10.4161/bact.24766","DOIUrl":"https://doi.org/10.4161/bact.24766","url":null,"abstract":"Four novel, closely related podoviruses, which displayed lytic activity against the gamma-proteobacterium Alteromonas macleodii, have been isolated and sequenced. Alterophages AltAD45-P1 to P4 were obtained from water recovered near a fish farm in the Mediterranean Sea. Their morphology indicates that they belong to the Podoviridae. Their linear and dsDNA genomes are 100-104 kb in size, remarkably larger than any other described podovirus. The four AltAD45-phages share 99% nucleotide sequence identity over 97% of their ORFs, although an insertion was found in AltAD45-P1 and P2 and some regions were slightly more divergent. Despite the high overall sequence similarity among these four phages, the group with the insertion and the group without it, have different host ranges against the A. macleodii strains tested. The AltAD45-P1 to P4 phages have genes for DNA replication and transcription as well as structural genes, which are similar to the N4-like Podoviridae genus that is widespread in proteobacteria. However, in terms of their genomic structure, AltAD45-P1 to P4 differ from that of the N4-like phages. Some distinguishing features include the lack of a large virion encapsidated RNA polymerase gene, very well conserved among all the previously described N4-like phages, a single-stranded DNA binding protein and different tail protein genes. We conclude that the AltAD45 phages characterized in this study constitute a new genus within the Podoviridae.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.24766","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31862921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 24

The moonlighting function of bacteriophage P4 capsid protein, Psu, as a transcription antiterminator. 噬菌体P4衣壳蛋白(Psu)作为转录抗终结者的兼职功能。

Bacteriophage Pub Date : 2013-04-01 DOI: 10.4161/bact.25657

Amitabh Ranjan, Ramanuj Banerjee, Bibhusita Pani, Udayditya Sen, Ranjan Sen

引用次数: 9

Evolution of genetic switch complexity. 遗传开关复杂性的进化。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24186

Gregory W Broussard, Graham F Hatfull

引用次数: 10

Biocontrol of Escherichia coli O157: H7 on fresh-cut leafy greens. 鲜切绿叶蔬菜O157: H7大肠杆菌的生物防治。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24620

Olcay Boyacioglu, Manan Sharma, Alexander Sulakvelidze, Ipek Goktepe

{"title":"Biocontrol of Escherichia coli O157: H7 on fresh-cut leafy greens.","authors":"Olcay Boyacioglu, Manan Sharma, Alexander Sulakvelidze, Ipek Goktepe","doi":"10.4161/bact.24620","DOIUrl":"https://doi.org/10.4161/bact.24620","url":null,"abstract":"The effect of a bacteriophage cocktail (EcoShield™) that is specific against Escherichia coli O157:H7 was evaluated against a nalidixic acid-resistant enterohemorrhagic E. coli O157:H7 RM4407 (EHEC) strain on leafy greens stored under either (1) ambient air or (2) modified atmosphere (MA; 5% O2/35% CO2/60% N2). Pieces (~2 × 2 cm2) of leafy greens (lettuce and spinach) inoculated with 4.5 log CFU/cm2 EHEC were sprayed with EcoShield™ (6.5 log PFU/cm2). Samples were stored at 4 or 10°C for up to 15 d. On spinach, the level of EHEC declined by 2.38 and 2.49 log CFU/cm2 at 4 and 10°C, respectively, 30 min after phage application (p ≤ 0.05). EcoShield™ was also effective in reducing EHEC on the surface of green leaf lettuce stored at 4°C by 2.49 and 3.28 log units in 30 min and 2 h, respectively (p ≤ 0.05). At 4°C under atmospheric air, the phage cocktail significantly (p ≤ 0.05) lowered the EHEC counts in one day by 1.19, 3.21 and 3.25 log CFU/cm2 on spinach, green leaf and romaine lettuce, respectively compared with control (no bacteriophage) treatments. When stored under MA at 4°C, phages reduced (p ≤ 0.05) EHEC populations by 2.18, 3.50 and 3.13 log CFU/cm2, on spinach, green leaf and romaine lettuce. At 10°C, EHEC reductions under atmospheric air storage were 1.99, 3.90 and 3.99 log CFU/cm2 (p ≤ 0.05), while population reductions under MA were 3.08, 3.89 and 4.34 logs on spinach, green leaf and romaine lettuce, respectively, compared with controls (p ≤ 0.05). The results of this study showed that bacteriophages were effective in reducing the levels of E. coli O157:H7 on fresh leafy produce, and that the reduction was further improved when produce was stored under the MA conditions.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4161/bact.24620","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31192111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Anna S. Tikhonenko: Electron microscopist extraordinary. Anna S. Tikhonenko:电子显微镜专家。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.23646

Vladimir I Popenko, Elizabeth M Kutter, Hans-W Ackermann

引用次数: 4

Bacteriophages for managing Shigella in various clinical and non-clinical settings. 在各种临床和非临床环境中用于控制志贺氏菌的噬菌体。

Bacteriophage Pub Date : 2013-01-01 Epub Date: 2013-05-21 DOI: 10.4161/bact.25098

Lawrence D Goodridge

引用次数: 24

Biochemical insights into the function of phage G1 gp67 in Staphylococcus aureus. 金黄色葡萄球菌噬菌体G1 gp67功能的生化分析。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24767

Joseph Osmundson, Seth A Darst

引用次数: 6

Phage-host interactions during pseudolysogeny: Lessons from the Pid/dgo interaction. 假溶解过程中噬菌体与宿主的相互作用：Pid/dgo 相互作用的启示。

Bacteriophage Pub Date : 2013-01-01 Epub Date: 2013-05-21 DOI: 10.4161/bact.25029

William Cenens, Angella Makumi, Mehari Tesfazgi Mebrhatu, Rob Lavigne, Abram Aertsen

引用次数: 0

Lytic bacteriophages reduce Escherichia coli O157: H7 on fresh cut lettuce introduced through cross-contamination. 溶菌噬菌体可减少通过交叉污染引入的新鲜生菜上的大肠杆菌 O157: H7。

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24323

Sean Ferguson, Cheryl Roberts, Eric Handy, Manan Sharma

{"title":"Lytic bacteriophages reduce Escherichia coli O157: H7 on fresh cut lettuce introduced through cross-contamination.","authors":"Sean Ferguson, Cheryl Roberts, Eric Handy, Manan Sharma","doi":"10.4161/bact.24323","DOIUrl":"10.4161/bact.24323","url":null,"abstract":"The role of lytic bacteriophages in preventing cross contamination of produce has not been evaluated. A cocktail of three lytic phages specific for E. coli O157:H7 (EcoShield™) or a control (phosphate buffered saline, PBS) was applied to lettuce by either; (1) immersion of lettuce in 500 ml of EcoShield™ 8.3 log PFU/ml or 9.8 log PFU/ml for up to 2 min before inoculation with E. coli O157:H7; (2) spray-application of EcoShield™ (9.3 log PFU/ml) to lettuce after inoculation with E. coli O157:H7 (4.10 CFU/cm2) following exposure to 50 μg/ml chlorine for 30 sec. After immersion studies, lettuce was spot-inoculated with E. coli O157:H7 (2.38 CFU/cm2). Phage-treated, inoculated lettuce pieces were stored at 4°C for and analyzed for E. coli O157:H7 populations for up to 7 d. Immersion of lettuce in 9.8 log PFU/ml EcoShield™ for 2 min significantly (p < 0.05) reduced E. coli O157:H7 populations after 24 h when stored at 4°C compared with controls. Immersion of lettuce in suspensions containing high concentrations of EcoShield™ (9.8 log PFU/ml) resulted in the deposition of high concentrations (7.8 log log PFU/cm2) of bacteriophages on the surface of fresh cut lettuce, potentially contributing to the efficacy of the lytic phages on lettuce. Spraying phages on to inoculated fresh cut lettuce after being washed in hypochlorite solution was significantly more effective in reducing E. coli O157:H7 populations (2.22 log CFU/cm2) on day 0 compared with control treatments (4.10 log CFU/cm2). Both immersion and spray treatments provided protection from E. coli O157:H7 contamination on lettuce, but spray application of lytic bacteriophages to lettuce was more effective in immediately reducing E. coli O157:H7 populations fresh cut lettuce.","PeriodicalId":8686,"journal":{"name":"Bacteriophage","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d4/25/bact-3-e24323.PMC3694057.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31192110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phage therapy: Should bacterial resistance to phages be a concern, even in the long run? 噬菌体治疗:即使从长远来看，细菌对噬菌体的耐药性是否值得关注?

Bacteriophage Pub Date : 2013-01-01 DOI: 10.4161/bact.24219

Anni-Maria Ormälä, Matti Jalasvuori

引用次数: 60