热带水库中感染环状水蛭和圆柱水蛭的噬藻体

Bacteriophage Pub Date : 2013-07-11 DOI:10.4161/bact.25571
B. Yeo, K. Gin
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引用次数: 9

摘要

从新加坡Kranji水库共分离出6个溶藻体。两个噬藻体感染了一种有毒的蓝藻——环状藻,而四个噬藻体感染了白茅蓝藻。为获得侵染环状假单胞菌和圆柱假单胞菌的噬藻菌,建立了两种分离方法。用孔法成功分离了侵染线虫的噬菌体,用双层菌斑法成功分离了侵染线虫的噬菌体。这些分离方法具有快速、显著的裂解效果。环状吸青体分离物可有效抑制环状吸青体的生长,具有潜在的生物防治潜力。在分离过程中,我们观察到,当与噬藻菌分离物接种时,圆柱孢杆菌的聚集和藻席形成发生了变化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cyanophages infecting Anabaena circinalis and Anabaena cylindrica in a tropical reservoir
A total of six lytic cyanophages have been isolated from Kranji Reservoir in Singapore. Two cyanophages infected a toxic species of cyanobacterium, A. circinalis, while four cyanophages infected the cyanobacterium A. cylindrica. To obtain the cyanophages that infect A. circinalis and A. cylindrica, two isolation methods were established. Cyanophages infecting A. circinalis were successfully isolated with well assay, and cyaophages infecting A. cylindrica were successfully isolated with double layer plaque assay. These isolation methods resulted in a rapid and significant lytic effect. A. circinalis cyanophage isolates are potential biocontrol of A. circinalis, due to the ability of cyanophage isolates in suppressing the growth of A. circinalis effectively. During the isolation process, we observed that A. cylindrica experienced changes in aggregation and algal mat formation when inoculated with the cyanophage isolates.
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