噬菌体K用于减少中心静脉导管材料上的金黄色葡萄球菌生物膜。

Bacteriophage Pub Date : 2013-10-01 Epub Date: 2013-10-24 DOI:10.4161/bact.26825
Matthew P Lungren, Diana Christensen, Ravi Kankotia, Irene Falk, Ben E Paxton, Charles Y Kim
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引用次数: 27

摘要

本项目的目的是确定噬菌体是否可以减少中心静脉导管材料上细菌的定植和生物膜的形成。用甲氧西林敏感金黄色葡萄球菌(0.5 McFarland标准)肉汤培养物接种20个硅胶片24 h。抽吸接种物,将接种盘分成两组,放置24 h:(1)对照组;(2)噬菌体处理(葡萄球菌噬菌体K,繁殖滴度> 108)。实验完成后,对培养皿进行定量培养。采用秩和检验进行统计检验。试验组平均菌落形成单位(CFU)显著低于对照组(对照组6.3 × 105 CFU,试验组6.7 × 101, P≤0.0001)。将噬菌体应用于生物膜感染的中心静脉导管材料可显著减少细菌定植和生物膜的存在。我们的数据表明,噬菌体治疗可能是解决中心静脉导管金黄色葡萄球菌生物膜感染的可行策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bacteriophage K for reduction of <i>Staphylococcus aureus</i>biofilm on central venous catheter material.

Bacteriophage K for reduction of Staphylococcus aureusbiofilm on central venous catheter material.

The purpose of this project was to determine whether bacteriophage can reduce bacterial colonization and biofilm formation on central venous catheter material. Twenty silicone discs were inoculated for 24 h with broth culture of Methicillin sensitive staphylococcus aureus (0.5 McFarland standard). The inoculate was aspirated and discs placed into two equal groups for 24 h: (1) untreated controls; (2) bacteriophage treatment (staphylococcal bacteriophage K, propagated titer > 108). At the completion of the experiment discs were processed for quantitative culture. Statistical testing was performed using the rank sum test. Mean colony forming units (CFU) were significantly decreased in experimental compared with controls (control 6.3 × 105 CFU, experimental 6.7 × 101, P ≤ 0.0001). Application of bacteriophage to biofilm infected central venous catheter material significantly reduced bacterial colonization and biofilm presence. Our data suggests that bacteriophage treatment may be a feasible strategy for addressing central venous catheter staph aureus biofilm infections.

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