Yuji Kishimoto, F. Okamae, Yukari Terashima, Miyoko Arimoto, M. Hosokawa, Misao Abe, M. Yamaoka, T. Otani, Setsuko Teranishi, M. Munakata, Hakuo Takahashi, S. Fukuhara
{"title":"OFF-HOURS EMERGENCY TRANSFUSION MANUAL OF UNCROSSMATCHED BLOOD","authors":"Yuji Kishimoto, F. Okamae, Yukari Terashima, Miyoko Arimoto, M. Hosokawa, Misao Abe, M. Yamaoka, T. Otani, Setsuko Teranishi, M. Munakata, Hakuo Takahashi, S. Fukuhara","doi":"10.3925/JJTC1958.51.571","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.571","url":null,"abstract":"We introduced an automated instrument for pretransfusion testing during off-hours based on column agglutination technology. As the time required for testing ABO group and Rh type is at least 20 minutes, we also prepared manuals regarding emergency transfusion of uncrossmatched blood. During a two-year period, 25 patients received 209 units (8.4 units per patient) of group O blood, and 19 patients received 289 units (15.2 units per patient) of group-specific blood. Additionally, 5 patients received 98 units of group-specific blood following group O blood. Fourteen patients (26.9%) required massive transfusion (20 or more units of blood within 24 hours). Accurate blood typing was carried out by using a blood sample drawn from the patient prior to infusion of group O blood. Antibody screening was carried out using this specimen the next day, and if a patient was positive for an antibody, complete compatibility testing was also performed. All patients were followed clinically and for any signs of transfusion reactions or crossmatching difficulties, but none developed. Although one patient had anti-E antibody present on admission and received E antigen-positive blood, no elevation of antibody level or delayed hemolytic reaction occurred. Two near-miss incidents associated with clerical errors were experienced with group-specific blood transfusion. We conclude that urgent transfusion of uncrossmatched blood is safe, and that group O blood transfusion may be safer than group-specific blood transfusion in some emergency situations. Off-hour manuals based on the time required for blood preparation will avoid transfusion errors.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"571-577"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336673","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Midori Kumagawa, K. Nibu, K. Kubota, Yuriko Nomaguchi, Y. Yoshiura
{"title":"BLOOD COMPONENT USAGE AT THE EMERGENCY AND CRITICAL CARE UNIT, FUKUOKA UNIVERSITY HOSPITAL","authors":"Midori Kumagawa, K. Nibu, K. Kubota, Yuriko Nomaguchi, Y. Yoshiura","doi":"10.3925/JJTC1958.51.430","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.430","url":null,"abstract":"We analyzed the usage of blood components at an Emergency and Critical Care Unit for 2 years, from 2001 to 2002. A total of 531 patients who received blood transfusions were divided into two groups, injured (group I; n=137 patients) and uninjured (group U; n=394 patients). Analysis of trigger points for transfusion revealed that group I showed statistically higher levels than those of group U, at 9.4 vs 8.6g/dl in Hemoglobin for red blood cell concentrates (RBCC), 69% vs 57% in prothrombin time for fresh frozen plasma (FFP) and 60, 000 vs 47, 000/μl in platelet counts for platelet concentrates. The trigger points in both groups for plasma transfusion were much higher than those recommended by the Government (30%). The FFP/RBCC ratios of transfused components were 0.31 in group I and 1.14 in group U. The cross-match/transfusion ratios were 1.3 in group I and 1.5 in group U, showing appropriate values. More than one kind of blood component was returned, however, during treatment in about one-half of group I and one-third of group U. Discussion of trigger points and other factors by the Blood Transfusion Committee is needed to determine the appropriate usage of blood components.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"430-434"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Norimi Niwa, T. Yuasa, S. Kimura, H. Tsuji, K. Yurugi, Ryoko Takegawa, R. Hishida, H. Egawa, Koichi Tanaka, Hiroaki Asano, T. Maekawa
{"title":"Identification of Preoperative Predictors of Intraoperative Blood Transfusion Requirements in Living Donor Liver Transplantation-Analysis of 772 Cases at a Single Center","authors":"Norimi Niwa, T. Yuasa, S. Kimura, H. Tsuji, K. Yurugi, Ryoko Takegawa, R. Hishida, H. Egawa, Koichi Tanaka, Hiroaki Asano, T. Maekawa","doi":"10.3925/JJTC1958.51.601","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.601","url":null,"abstract":"Living-donor liver transplantation (LDLT) is now an important option for the treatment of patients with end-stage or irreversible liver disease. We have previously reported the quantities of blood required for transplantation in 772 cases of LDLT performed from June 1990 to March 2002 at Kyoto University Hospital. In the present study we retrospectively analyzed the preoperative factors associated with massive blood losses during LDLT. The patients were divided into two groups, with the upper quartile was defined as the high blood loss (HBL) group and the lower three quartiles as the low blood loss (LBL) group. Preoperative variables between the groups were compared and statistically analyzed by chi-square analysis or the Mann-Whitney U-test. Results showed that predictors of HBL were age ( 2.0g/dl), T-Bil (>10.0g/dl), D-Bil (>10.0g/dl), Cre (>1.0g/dl) and BUN (>30.0g/dl); pre-operative hospitalization; re-transplantation; and diagnosis (Biliary atresia, Budd-Chiari syndrome). In conclusion, particular care against the possibility of massive intra-operative blood loss should be taken in patients with low age, anemia, severe liver dysfunction, and renal dysfunction.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"601-608"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70337282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Koji Yamamoto, Satoshi Suzuki, Masayoshi Kobayashi, T. Kondo, K. Komori, T. Murohara, J. Takamatsu
{"title":"THERAPEUTIC ANGIOGENESIS BY BONE MARROW MONONUCLEAR CELL TRANSPLANTATION AND QUANTITATIVE EVALUATION OF ENDOTHELIAL PROGENITOR CELLS IN PATIENTS WITH PERIPHERAL OBSTRUCTIVE ARTERIAL DISEASE","authors":"Koji Yamamoto, Satoshi Suzuki, Masayoshi Kobayashi, T. Kondo, K. Komori, T. Murohara, J. Takamatsu","doi":"10.3925/JJTC1958.51.537","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.537","url":null,"abstract":"We performed therapeutic angiogenesis by cell transplantation (TACT) for patients with peripheral obstructive arterial disease using autologous bone marrow mononuclear cells. The patients' symptom has begun to improve within several days after therapy, as did laboratory data (i. e., anklebrachial blood pressure index, thermography, percutaneous measurement of oxygen pressure). We quantified the mRNA expression of endothelial progenitor cell (EPC) -specific molecules (e. g., Flk-1, CD133, VE-cadherin, etc.) in bone marrow- or peripheral blood-derived mononuclear cells obtained from patients with ischemic limbs, using real time reverse transcription-PCR. The mRNA expression level of EPC markers was significantly lower in the patients than in healthy controls. We furthermore revealed the different expression pattern of EPC markers in several sources, including bone marrow and peripheral blood obtained from healthy volunteers or recombinant G-CSF-treated donors. The finding that the expression of EPC-specific molecules was decreased in the patients' marrow and blood suggests that patients with peripheral obstructive arterial diseases may have lower angiogenic potential. However, TACT with autologous marrow mononuclear cells was effective and increased the number of circulating EPCs in the patients' blood.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"537-542"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Saito, Yumi Komatsu, S. Ota, K. Tamaki, Hideyuki Seshimo, Yuichiro Yamazaki, S. Nomura
{"title":"Analysis of an Hla-B Mosaicism Observed in Apheresis Donor","authors":"S. Saito, Yumi Komatsu, S. Ota, K. Tamaki, Hideyuki Seshimo, Yuichiro Yamazaki, S. Nomura","doi":"10.3925/JJTC1958.51.530","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.530","url":null,"abstract":"We encountered an apheresis donor with a suspected case of mosaicism. The donor, K. A., was a 46-year-old Japanese female registered as an HLA-compatible platelet concentrate (HLA-PCs) donor. In her HLA serological typing for HLA-PCs donor registration, three antigens, HLA-B55, -B61 and -B62, were assigned in the HLA-B locus. She was not a twin and had never undergone transfusion or transplantation. Flow cytometric analysis using platelets and lymphocytes indicated that HLA-B55, -B61 and -B62 antigens were simultaneously expressed on the surface of platelets and lymphocytes. HLA-B allele typing with the polymerase chain reaction restriction fragment length polymorphism technique using genomic DNA from peripheral blood and nails as a somatic sample was carried out to establish HLA-B mosaicism. Three alleles, HLA-B*5502, -B*4002 and B*1501, were assigned in both exon 2 and exon 3. HLA-B mosaicism was observed in both her peripheral blood and nails. The HLA-B*4002 and -B*1501 alleles were inherited by her son, who carried three HLA-B alleles, HLA-B*4002, -B*1501 and -B*4001. No other sign of chimerism or mosaicism was observed in other HLA loci antigens or red cell antigens. These results suggest that this donor has a chimeric allele consisting of a mixture of the nucleotide sequences of HLA-B*4002 and -B*1501 alleles.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"530-536"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Kawashima, K. Irita, K. Morita, K. Tuzaki, T. Sawa
{"title":"PREOPERATIVE HEMORRHAGIC SHOCK AND INTRAOPERATIVE BLEEDING: TWO MAIN CAUSES OF SURGICAL DEATHS IN JAPAN","authors":"Y. Kawashima, K. Irita, K. Morita, K. Tuzaki, T. Sawa","doi":"10.3925/JJTC1958.51.23","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.23","url":null,"abstract":"The Japanese Society of Anesthesiologists (JSA) Subcommittee on Surveillance of Anesthesiarelated Critical Incidents sent confidential questionnaires to all JSA Certified Training Hospitals (n=806 on average) every year from 1999 to 2002. Data collected in the identical forms each year were analyzed for incidence of cardiac arrest and other critical events during anesthesia and surgery, and for outcomes within 7 postoperative days. The principal cause of each critical incident selected from a list of 52 items provided on the questionnaires was also analyzed.With an average response rate of 75.0%, a total of 4, 297, 066 cases were documented over 4 years. A total of 2, 860 patients died within 7 postoperative days. The two principal causes of deaths were preoperative hemorrhagic shock and massive hemorrhage during surgery (Fig. 2), representing 32.8% [95% CI at p<0.05: 31.0, 34.6] and 18.0% [15.1, 20.8] of cases, respectively (Fig. 3). The two principal causes of cardiac arrest during surgery and anesthesia were valso preoperative hemorrhagic shock and massive hemorrhage during surgery (Fig. 4). To reduce life-threatening hemorrhagic events in the operating room and mortality and morbidity within 7 postoperative days in Japan, analysis is need of causes of perioperative bleeding; sequences leading to fatality; adequacy of staffing levels in the operating room; as well as the functional state of the blood supply system from a blood bank, through transfusion service of the hospital to the operating room.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"23-31"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3925/JJTC1958.51.23","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Nagamine, Masako Takahashi, Kazuyoshi Watanabe, T. Tokuda, T. Fujii, Kei Tanaka, K. Yuki
{"title":"EFFICIENCY OF A CHECK SYSTEM FOR INFECTION AFTER BLOOD TRANSFUSION","authors":"S. Nagamine, Masako Takahashi, Kazuyoshi Watanabe, T. Tokuda, T. Fujii, Kei Tanaka, K. Yuki","doi":"10.3925/JJTC1958.51.32","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.32","url":null,"abstract":"At our institution, only 22% of patients receiving blood transfusions between May and October, 2002, were subsequently screened for HIV infection. To improve this rate, we prepared lists of all patients undergoing transfusion on a monthly basis and distributed them to the attending department. We changed the principle of the system to further improve the rate in May of 2003 by the use of new individual forms programmed using commercial database software (FileMaker Pro) for each patient. The new forms featured an easily readable design and were distributed to the attending department even after discharge. The frequency of screening after blood transfusion remarkably increased between May and October, 2003, to 77%. We concluded that form design, particularly readability for individual patients, and method of notification are important for effective verification of transfusionassociated HIV infection.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"32-37"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Igari, H. Ohto, T. Ogata, K. Ikeda, Kaoru Sato, T. Takagi, K. Takeyama, Shigeo Aota, S. Kikuchi
{"title":"CLINICAL TRIAL OF PRESTORAGE LEUKOCYTE REDUCTION FOR AUTOLOGOUS BLOOD TRANSFUSION","authors":"T. Igari, H. Ohto, T. Ogata, K. Ikeda, Kaoru Sato, T. Takagi, K. Takeyama, Shigeo Aota, S. Kikuchi","doi":"10.3925/JJTC1958.50.704","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.704","url":null,"abstract":"The goal of the present study was to determine the efficacy of reducing the number of leukocytes in autologous blood before storage. The study sample consisted of 121 patients undergoing orthopedic surgery and scheduled to receive up to 1, 200mL autologous blood. They were divided into prestorage leukocyte reduction and no-reduction groups. Body temperature, white blood cell counts, red blood cell counts, and C reactive protein (CRP) levels were compared after operation. Thirteen of 121 patients were excluded from comparison due to rheumatic etiology. Results showed no significant difference in efficiency between the leukocyte-reduced (n=46) and non-reduced (n=62) groups. The leukocyte reduction group showed slightly lower CRP levels for 48h after surgery than the noreduction group. The benefits of prestorage leukocyte reduction of autologous blood remains to be elucidated.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"704-709"},"PeriodicalIF":0.0,"publicationDate":"2004-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}