Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion最新文献

MAINTENANCE OF HIGH FACTOR VIII ACTIVITY IN SOURCE PLASMA FROZEN AND STORED IN THE MPIII AND MPIIIs PORTABLE FREEZING SYSTEMS 维持源血浆在MPIII和MPIII便携式冷冻系统中冷冻和储存的高因子VIII活性

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.37.830

Kimiko Segawa, S. Chiba, Tsuneo A. Takahashi, T. Nakase, S. Sekiguchi

{"title":"MAINTENANCE OF HIGH FACTOR VIII ACTIVITY IN SOURCE PLASMA FROZEN AND STORED IN THE MPIII AND MPIIIs PORTABLE FREEZING SYSTEMS","authors":"Kimiko Segawa, S. Chiba, Tsuneo A. Takahashi, T. Nakase, S. Sekiguchi","doi":"10.3925/jjtc1958.37.830","DOIUrl":"https://doi.org/10.3925/jjtc1958.37.830","url":null,"abstract":"MAINTENANCE OF HIGH FACTOR VIII ACTIVITY IN SOURCE PLASMA FROZEN AND STORED IN THE MPIII AND MPIIIs PORTABLE FREEZING SYSTEMS Kimiko Segawa, Seiji Chiba, Tsuneo A. Takahashi, Toshie Nakase and Sadayoshi Sekiguchi Hokkaido Red Cross Blood Center A new freezing system for preparing high quality source plasma was investigated. The MPIIITM portable freezing system consists of a freezer called the \"Cell Unit\" and a storage case. The MPIII cell unit and the MPIIIs cell unit, about half the size of the MPIII, were transferred to storage cases after \"recharging\" (storage at -40C) and their ability to freeze apheresis plasma bags was tested. The MPIII and the MPIIIs freezers could freeze 8 and 4 plasma bags respectively to a temperature below -20C. However, the MPIII could freeze 16 bags and the MPIIIs 9 bags, before the cell unit temperature rose up to -20C. They could freeze and store plasma bags below -20C for about one day when there were 12 bags in the MPIII or 6 bags in the MPIIIs. The recovery of factor VIII activity (FVIII: C) of the plasma frozen in the MPIIIs was high (97%) and was similar to that of plasma frozen in a blast freezer. This high FVIII: C did not decrease even after overnight storage in the MPIIIs. In addition, the MPIIITM portable freezing system has several other merits. No electricity is required after \"recharging\" the cell unit, it is safe because neither liquid nitrogen nor ethanol is required and the plasma bags are flat after freezing, which allows easy bar code reading and safe, efficient storage and transportation. Thus it is possible for the MPIII and MPIIIs systems to freeze the collected plasma at blood collection sites and to retain high FVIII: C.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70315690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BUFFY COAT DEPLETED CRC ) THROUGH ONE TYPE OF LEUKOCYTE REMOVAL FILTER 通过一种类型的白细胞去除过滤器，褐色涂层耗尽CRC

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.39.732

I. Aoki, Yukari Takeda, Shizuko Egami, Miho Takahashi, N. Furuya, K. Nakahara, Michio Kawashima, M. Tsubokura, K. Aoyama

{"title":"BUFFY COAT DEPLETED CRC ) THROUGH ONE TYPE OF LEUKOCYTE REMOVAL FILTER","authors":"I. Aoki, Yukari Takeda, Shizuko Egami, Miho Takahashi, N. Furuya, K. Nakahara, Michio Kawashima, M. Tsubokura, K. Aoyama","doi":"10.3925/jjtc1958.39.732","DOIUrl":"https://doi.org/10.3925/jjtc1958.39.732","url":null,"abstract":"The ability of leukocyte removal filters was investigated with three types of concentrated red blood cells (CRC); i.e., CRC prepared by a standard method (N-CRC), CRC suspended in Map (MapCRC) and buffy coat depleted CRC (BD-CRC). Pall RC50 filters with the same lots were used, and the same procedures were done in filtration to avoid the variations in filters as much as possible. Two units of each CRC stored for 1, 6 and 10 days was filtered through Pall RC50 filters. The mean residual leukocyte counts (n=12), which were calculated from the sum of each CRC stored for 1, 6 and 10 days, were as follows. For the 1st unit of N-CRC, Map-CRC and BD-CRC were 2.66 + 1.28 (X105), 1.55 + 0.25 (X105) and 1.44 + 0.25 (X105), respectively. For the 2nd units of BD-CRC, the mean count was 1.84 + 0.91(X 105), and 1.42 + 1.72 (X 106) for Map-CRC, while it was 6.58 + 105 to 1.38 X 108 [2.28 + 4.02 (X 107)] for N-CRC, showing much variation. The filter was obstructed with much microaggregate in one N-CRC sample stored for 10 days. The residual leukocyte count after filtration with this filter was high (8.16X107). These data suggest that the individual differences in the donor have a strong influence on the ability of leukocyte removal in N-CRC. BD-CRC and Map-CRC are more useful than N-CRC to bring out the ability of a leukocyte removal filter.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70318195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SEROLOGICAL FEATURES OF ANTIBODIES TO COMBINED ABH-LEWIS ANTIGENS PRODUCED IN A PATIENT AFTER OPEN-HEART SURGICAL OPERATION 心内直视手术后患者产生abh-lewis联合抗原抗体的血清学特征

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.39.800

H. Yasuda, H. Ohto, K. Hirahara, Y. Tohyama, T. Tasaki, Ryoichi Motokil, S. Arai, Tsuneyoshi Saito

{"title":"SEROLOGICAL FEATURES OF ANTIBODIES TO COMBINED ABH-LEWIS ANTIGENS PRODUCED IN A PATIENT AFTER OPEN-HEART SURGICAL OPERATION","authors":"H. Yasuda, H. Ohto, K. Hirahara, Y. Tohyama, T. Tasaki, Ryoichi Motokil, S. Arai, Tsuneyoshi Saito","doi":"10.3925/jjtc1958.39.800","DOIUrl":"https://doi.org/10.3925/jjtc1958.39.800","url":null,"abstract":"SEROLOGICAL FEATURES OF ANTIBODIES TO COMBINED ABH-LEWIS ANTIGENS PRODUCED IN A PATIENT AFTER OPEN-HEART SURGICAL OPERATION Hiroyasu Yasuda, Hitoshi Ohto, Kazuko Hirahara, Yuriko Tohyama, Tetsunori Tasaki, Ryoichi Motokil, Satoru Arai2 and Tsuneyoshi Saito3 Blood Transfusion Service, Fukushima Medical College 2Department of Cardiac Surgery, Tohoku Kousai Hospital 3Department of Internal Medicine, Yonezawa Municipal Hospital We found antibodies seropositive for lymphocytoxicity test (LCT) in a 60-year-old male patient, underwent an aorto-coronary bypass surgery for angina pectoris. He had received 800 ml of autologous blood, 940 ml of 25% human albumin and 720 mg of fibrin glue. Anti-human leukocyte antigen (HLA) antibody is frequently produced as a result of alloimmunization induced by homologous blood transfusions and even infusion of human plasma preparations including soluble HLA antigens. LCT reactive antibodies produced in the patient were proved to be not against HLA, as they were not absorbed by pooled platelets from type 0 blood group multiple donors, nor reacted with any of 18 B-cell lines. The antibodies reacted only with panel T-cells from A Led, B Led donors. These antibodies identified were anti-A and -B, mainly combined ABHLewis antigens, as the results demonstrated by lymphocytotoxicity test using the patient's sera after absorption with homologous red blood cells, lymphocytes, platelets, and with columns coated by A, B and Lewis substances. The antibodies seem to be induced by some human plasma preparations with soluble ABO blood group antigens infused during perioperative period, although, details of the mechanism remain obscure.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70318840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EVALUATION OF AUTOMATED SYPHILIS SCREENING FOR BLOOD DONORS USING TREPONEMA PALLLIDUM PARTICLE AGGLUTINATION (TPPA) TEST 用梅毒螺旋体颗粒凝集(tppa)试验对献血者进行梅毒自动筛查的评价

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.42.265

S. Sato, S. Kishimoto, Kazuhiro Kuzuma, Ryouichi Maeda, T. Kato, H. Ikeda, S. Sekiguchi

{"title":"EVALUATION OF AUTOMATED SYPHILIS SCREENING FOR BLOOD DONORS USING TREPONEMA PALLLIDUM PARTICLE AGGLUTINATION (TPPA) TEST","authors":"S. Sato, S. Kishimoto, Kazuhiro Kuzuma, Ryouichi Maeda, T. Kato, H. Ikeda, S. Sekiguchi","doi":"10.3925/jjtc1958.42.265","DOIUrl":"https://doi.org/10.3925/jjtc1958.42.265","url":null,"abstract":"Sera obtained from six patients with recently infected syphilis showed positive reaction by Treponema pallidum Particle Agglutination (TPPA, Fujrebio), a newly developed test for syphilis infection. The results were confirmed by other immunoassays such as enzyme immunoassay (Imzyn M-TP, Fujirebio) and/or immunofluorescence (FTA-ABS, Nihon Touketukansou). In contrast, they were negative by Serodia-TP (Fujirebio). In a seroconversion panel, TPPA showed positive reaction earlier than conventional RPR (rapid plasma reagin, Kaketuken). IgM fractions prepared from five recently infected patients were all positive by TPPA, while only one was positive by Serodia-TP. These results indicate that TPPA is able to detect anti-TP in the early phase of infection. In the next step, we evaluated the application of this test for blood donor screening with a PK7200 automated pretransfusion analyzer for agglutination assays (Olympus). After establishing optinal conditions for TPPA in the PK7200 (PK-TPPA), 4,902 samples were simultaneously tested by manual and automated testing. Positive samples were manually retest ed. There was no false negative results by the PK test, while both the manual PK tests yielded a similar rate of false positive results. In a simultaneous test with PK-TPPA and RPR, respec tively 43 and 65 of 14,106 donor samples were repeatedly positive. Of 21 samples showing positive reaction in both tests, 17 were confirmed as IgG type anti-TP by FTA-ABS. Of 20 PK-TPPA(+)RPR(-) samples, 7 were IgG type anti-TP positive. None of the 44 RPR(+)-TPPA(-) samples were positive by FTA-ABS. We conclude that the PK-TPPA is sensitive enough to detect IgM type anti-TP and acceptable for donor screening.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3925/jjtc1958.42.265","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70325049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRADYKININ GENERATION IN RED CELL CONCENTRATE FILTERED WITH LEUKOCYTE-REDUCTION FILTERS 用白细胞减少过滤器过滤的红细胞浓缩物中缓激肽的产生

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.42.258

Yoshiko, Ohnishi, Tsuneo, A. Takahashi, M. Hosoda, Hideki, Abe, K. Ikebuchi, Sadayoshi, Sekiguchi

{"title":"BRADYKININ GENERATION IN RED CELL CONCENTRATE FILTERED WITH LEUKOCYTE-REDUCTION FILTERS","authors":"Yoshiko, Ohnishi, Tsuneo, A. Takahashi, M. Hosoda, Hideki, Abe, K. Ikebuchi, Sadayoshi, Sekiguchi","doi":"10.3925/jjtc1958.42.258","DOIUrl":"https://doi.org/10.3925/jjtc1958.42.258","url":null,"abstract":"BRADYKININ GENERATION IN RED CELL CONCENTRATE FILTERED WITH LEUKOCYTE-REDUCTION FILTERS Yoshiko Ohnishi, Tsuneo A. Takahashi, Mani Hosoda, Hideki Abe, Kenji Ikebuchi and Sadayoshi Sekiguchi Hokkaido Red Cross Blood Center We have previously reported bradykinin (BK) generation during filtration of platelet concen trates with leukocyte-reduction filters made of polyester fibers with a negative zeta-potential through contact activation. In the present study we investigated whether BK was generated when red cell concentrates containing MAP (RC-MAP) was filtered with 7 different leukocytereduction filters (Filters A-G) made for red cell concentrates. Mean BK level in RC-MAP stored one day was 39.8-78.8 pg/m l before filtration. However, concentration increased several thousand-fold to ng/ml levels during filtration with Filters A-F with a negative zeta-potential. In contrast, generation of BK was suppressed during filtration with Filter G with a positive zeta-potential to a maximum level of 601-350 pg/ml. As the pre-filtration storage period of RC-MAP was extended, BK levels after filtration with Filter A decreased with 95% suppression and almost no BK generation observed in RC-MAP stored for 20 or 43 days. As the post-filtration storage period of RC-MAP was extended, BK levels after filtration with Filter A decreased to pre-filtration levels in 3 hours. Further, kal likrein levels in RC-MAP increased significantly and high molecular weight kininogen levels decreased after filtration with Filter A. The clinical significance of this BK generation during filtration remains unclear, but should be considered with regard to the clinical application of leukocyte-reduction filters.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70325117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

THE DISTRIBUTION OF HLA-A, B, C, Bf, C4 AND TWO LOCUS ASSOCIATION OF HEALTHY APHERESIS DONNOR IN YAMAGUCHI PREFECTURE, WESTERN AREA OF JAPAN 日本西部山口县健康献血者HLA-A、B、C、Bf、C4及2个基因座关联的分布

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.34.313

Hidenori Tanaka, K. Itoh, K. Hirakawa, Yamaguchi

引用次数: 0

BIOCHEMICAL PROPERTIES OF FACTOR VIII IN HIGHLY-PURIFIED FACTOR VIII CONCENTRATES (CROSS EIGHT M) PRODUCED BY MONOCLONAL ANTIBODY COLUMN 单克隆抗体柱制备的高纯度viii因子浓缩物(交叉8 m)的生化特性

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.38.51

M. Furuta, Isao Takahashil, Fumie Yakol, S. Mizuno, T. Kamiya, Tsuyoshi Murozuka, H. Ito, S. Sekiguchi

{"title":"BIOCHEMICAL PROPERTIES OF FACTOR VIII IN HIGHLY-PURIFIED FACTOR VIII CONCENTRATES (CROSS EIGHT M) PRODUCED BY MONOCLONAL ANTIBODY COLUMN","authors":"M. Furuta, Isao Takahashil, Fumie Yakol, S. Mizuno, T. Kamiya, Tsuyoshi Murozuka, H. Ito, S. Sekiguchi","doi":"10.3925/jjtc1958.38.51","DOIUrl":"https://doi.org/10.3925/jjtc1958.38.51","url":null,"abstract":"Biochemical properties of Factor VIII (FVIII) in highly-purified FVIII concentrates (CROSS EIGHT M) produced by monoclonal antibody column were studied and compared with those of Hemofil M which was already used clinically. The FVIII clotting activity (FVIII:Cclot) levels obtained by one-stage method using severe hemophilia A plasma as a substrate were 614729 U/vial and identical with those indicated on the label (500U/vial). The FVIII light chain antigen (FVIIIL:Ag) levels measured by Enzyme Immunoassay were 540-760U/vial. The ratio of FVIIIL:Ag to FVIII:Cclot (FVIIIL:Ag/FVIII:Cclot) were 0.88-1.04. The ratio of FVIIIL:Ag to von Willebrand Factor antigen (vWF:Ag) (FVIIIL:Ag/vWF:Ag) were 49.0-60.8. No difference was observed between CROSS EIGHT M and Hemofil M on the FVIII:Cclot levels, FVIIIL:Ag levels, FVIIIL:Ag/FVIII:Cclot and FVIIIL: Ag/vWF: Ag. SDS-PAGE patterns showed almost no contamination except albumin being added as a stabilizer. Western blot analysis revealed FVIII heavy chain antigen of M. W. 90 kDa-220 kDa and FVIII light chain antigen of M. W. 80 kDa. No degradates of MW. 43 kDa-50 kDa derived from FVIII heavy chain antigen were observed. CROSS EIGHT M lacked the large multimers of vWF which were detected in normal human pooled plasma. No difference was observed between CROSS EIGHT M and Hemofil M by western blot analysis of FVIII and multimer analysis of vWF. These results suggest that CROSS EIGHT M preserves the fundamental structure of FVIII molecule for coagulant activity. Therefore, CROSS EIGHT M as well as Hemofil M may be clinically used with safety as FVIII concentrates.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70316681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

COMPARATIVE STUDY OF SERUM GUANASE AND ALT ACTIVITIES IN THE OBESE 肥胖者血清谷丙转氨酶和酶活性的比较研究

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.41.347

H. Hayashi, S. Hibino, T. Nishikado, H. Honda

{"title":"COMPARATIVE STUDY OF SERUM GUANASE AND ALT ACTIVITIES IN THE OBESE","authors":"H. Hayashi, S. Hibino, T. Nishikado, H. Honda","doi":"10.3925/jjtc1958.41.347","DOIUrl":"https://doi.org/10.3925/jjtc1958.41.347","url":null,"abstract":"To evaluate serum guanase (GU) activity in the obese, serum alanine aminotransf erase (ALT) and GU activities in donor and patient bloods were investigated in terms of obesity. In donor blood, serum ALT activity was significantly higher as the degree ofobesity inceased, while serum GU activity showed no significant difference. Serum ALT activity was abnormal in 2.2 and serum GU activity in 2.0%, but little correspondence was seen between ALT-abnormal blood and GU-abnormal blood and many ALT-abnormal donors were obese. In patient blood, serum ALT and GU activities in patients with fatty liver were significantly higher than in healthy persons; however, mean ALT activity was beyond the normal range, while mean GU activity remained within the normal range. In the blood of patients without chronic hepatitis, liver cirrhosis, hepatocellular carcinoma and alcoholic liver disease, serum ALT activity was significantly higher with the degree of obesity, while serum GU activity showed no significant relationship to obesity. These results suggested that elevation of serum ALT activity caused by obesity could be differentiated by measurement of serum GU activity.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70323917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

SELECTION OF HLA-HPA-MATCHED BLOOD DONORS BY HPA-DNA TYPING WITH FROZEN LYMPHOCYTES ON HLA-MATCHED DONORS hla - hpa-dna分型选择hla - hpa匹配的献血者

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.43.883

K. Morishita, T. Mitani, S. Sato, T. Kato, T. Nakase, H. Ikeda, S. Sekiguchi

{"title":"SELECTION OF HLA-HPA-MATCHED BLOOD DONORS BY HPA-DNA TYPING WITH FROZEN LYMPHOCYTES ON HLA-MATCHED DONORS","authors":"K. Morishita, T. Mitani, S. Sato, T. Kato, T. Nakase, H. Ikeda, S. Sekiguchi","doi":"10.3925/jjtc1958.43.883","DOIUrl":"https://doi.org/10.3925/jjtc1958.43.883","url":null,"abstract":"HPA-alloimmunization is a major factor in refractoriness to HLA-matched platelet transfusion. Retrospective studies indicate that although HPA-matched platelet transfusion is effective in such cases, it is extremely difficult to deal with such situations prospectively. We report a case of successful transfusion of HPA-matched platelets selected by HPA-DNA typing with frozen lymphocytes of HLA-matched donors. The patient was a 51-year-old male with CML who developed HLA antibodies after multiple trnasfusion of random platelets. However, HLAmatched platelets were ineffective. Further serological tests including monoclonal antibodyspecific immobilization of platelet antigens (MAIPA) revealed that he also possessed HPA-3a antibody, which appeared to be responsible for the refractoriness. HPA-DNA typing by the PCR-SSP method indicated that his HPA-3 type was b/b and those of refractory donors were HPA-3a/a or HPA-3a/b. By HPA-3 typing with 122 frozen lymphocytes of HLA-matched donors, 14 donors were found to be HPA-3b/b. HLA-HPA matched platelets from these donors were shown to be effective, indicating that refractoriness in this patient was due to HPA-3aalloimmunization. The method described here for selection of HLA-HPA matched donors is effective under the following circumstances: 1) a considerable number of HLA-matched donors and their frozen lymphocytes are available; 2) HPA matching frequency of a given case is not rare; and 3) transfusion of matched platelets is expected to be terminated in a limited period.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70326970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ANALYSIS OF HEPATITIS C VIRUS CARRIERS DERIVED FROM BLOOD DONORS -From the Epidemiological and Clinicopathological Viewpoint- 献血者丙型肝炎病毒携带者的流行病学和临床病理学分析

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2010-01-01 DOI: 10.3925/jjtc1958.40.454

Hiromi, Ihara, Toshiaki, Kato, Toshie, Nakase, Hisami, Ikeda

{"title":"ANALYSIS OF HEPATITIS C VIRUS CARRIERS DERIVED FROM BLOOD DONORS -From the Epidemiological and Clinicopathological Viewpoint-","authors":"Hiromi, Ihara, Toshiaki, Kato, Toshie, Nakase, Hisami, Ikeda","doi":"10.3925/jjtc1958.40.454","DOIUrl":"https://doi.org/10.3925/jjtc1958.40.454","url":null,"abstract":"The 501 HCV carriers (273 males, 228 females) detected through blood donations from May 1991 to Jan.1993 were followed for the average of 12. 3 months in our carrier clinic, and those who were indicated for therapy were referred to specialized hospitals. Transfusion history was found in 21% of male carriers and in 39% of female carriers, of which only 16% had suffered from post-transfusion hepatitis.Family history of HCV related liver disease was found in 34 cases (7%). Approximately 70% of the carriers showed normal GPT during the follow-up period.Some of them (11 cases), in which other liver function tests or ECHO showed abnormal findings, were diagnosed to be chronic hepatitis histologically.Analysis of the 45 biopsy cases revealed CPH (8 cases), CAH2A (29 cases), CAH2B (6 cases) and LC (2 cases).Clinical background such as age and period from infection, GPT change and ECHO score differed between the group of CPH and CAH2A, and the group of CAH2B and LC.On the other hand, HCV genotype and HCV-RNA titer did not differ significantly between the two groups. It is concluded that medical check-up of asymptomatic HCV carriers detected through blood donations is important even if their GPT stays normal, and will be useful for early diagnosis and treatment of chronic hepatitis.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2010-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70320307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0