Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion最新文献_第6页

RAPID QUANTITATION OF IgG ANTIBODIES SPECIFIC FOR BLOOD GROUP ANTIGENS A AND B BY SURFACE PLASMON RESONANSE 用表面等离子共振法快速测定血型抗原A和B的IgG抗体

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/jjtc1958.51.545

K. Yurugi, S. Kimura, T. Yuasa, H. Segawa, J. Kuroda, Kiyoshi Sato, M. Nogawa, H. Egawa, K. Tanaka, T. Maekawa

{"title":"RAPID QUANTITATION OF IgG ANTIBODIES SPECIFIC FOR BLOOD GROUP ANTIGENS A AND B BY SURFACE PLASMON RESONANSE","authors":"K. Yurugi, S. Kimura, T. Yuasa, H. Segawa, J. Kuroda, Kiyoshi Sato, M. Nogawa, H. Egawa, K. Tanaka, T. Maekawa","doi":"10.3925/jjtc1958.51.545","DOIUrl":"https://doi.org/10.3925/jjtc1958.51.545","url":null,"abstract":"The measurement of anti-blood group A/B (anti-A/B) IgG antibody levels is important for ABO unmatched-organ recipients because the effective removal of the antibodies improves their prognosis. However, currently existing methods to detect anti-A/B IgG antibodies suffer limitations due to high costs, low throughput, and poor adaptability to automation. We have developed a rapid means to quantitate anti-A/B IgG antibodies by surface plasmon resonance (SPR). The change in titers when the same plasma was diluted was precisely reflected by the SPR method. The coefficients of correlation between SPR and test tube (TT) methods for anti-A and -B IgG antibodies were 0.85 and 0.56, respectively. The SPR values also paralleled the TT values that showed a decline in titers after the removal of antibodies by double filtration plasma apheresis or plasma exchange.**This article is partly based on a study first reported in the Transfusion (Kimura, S., Yurugi, K., Yuasa, T., Tsuji, H., Segawa, H., Kuroda, J., Sato, K., Nogawa, M., Egawa, H., Tanaka, K., Maekawa, T.: Rapid quantitation of IgG antibodies specific for blood group antigens A and B by surface plasmon resonance. Transfusion, 45 (1): 56-62, 2005.)","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"19 1","pages":"545-552"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A CASE OF DELAYED HEMOLYTIC TRANSFUSION REACTION DUE TO ANTI-C+e ANTIBODY CAUSED BY PRIMARY IMMUNE RESPONSE 原发性免疫反应引起的抗c +e抗体延迟溶血性输血反应1例

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.594

J. Kitazawa, M. Inomata, C. Kamata, K. Tanaka, K. Hosokawa, Hirobumi Saito, Tomonori Murakami, M. Ikuta, A. Takemura, S. Hasegawa, T. Mikami

{"title":"A CASE OF DELAYED HEMOLYTIC TRANSFUSION REACTION DUE TO ANTI-C+e ANTIBODY CAUSED BY PRIMARY IMMUNE RESPONSE","authors":"J. Kitazawa, M. Inomata, C. Kamata, K. Tanaka, K. Hosokawa, Hirobumi Saito, Tomonori Murakami, M. Ikuta, A. Takemura, S. Hasegawa, T. Mikami","doi":"10.3925/JJTC1958.51.594","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.594","url":null,"abstract":"We report here a rare case of delayed hemolytic transfusion reaction (DHTR) probably caused by a primary immune response. The patient was a 69-year-old Japanese female who underwent neurosurgery for subarachnoid hemorrhage. For treatment of post-operative hemorrhage, she received 2 units of packed red cells (Ir-RC-MAP) per day for 6 days, for a total of 12 units. On day 33 after the first transfusion, she showed symptoms of hemoglobinuria, anemia, thrombocytopenia, elevated levels of serum LDH and total bilirubin, and a decreased level of serum haptoglobin. Her blood was typed as A, ccDEE. Partial coagulation was observed on Rh blood-type examination for C and e but disappeared on day 37 after the first transfusion. On irregular antibody screening of patient serum collected 37 days after the first transfusion, IgM type anti-C+e was detected by MTS-Pap. We found that the immunoglobulin class of anti-C+e switched from IgM to IgG during the clinical course. These findings suggest that the DHTR was probably due to anti-C+e caused by a primary immune response.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"594-600"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70337153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

CHARACTERISTICS OF RISK BEHAVIOR AMONG HIV-POSITIVE VOLUNTARY BLOOD DONORS AND MEASURES ENSURING BLOOD SAFETY IN JAPAN 日本hiv阳性自愿献血者的危险行为特征及确保血液安全的措施

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/jjtc1958.51.333

M. Shimizu, M. Takenaka

{"title":"CHARACTERISTICS OF RISK BEHAVIOR AMONG HIV-POSITIVE VOLUNTARY BLOOD DONORS AND MEASURES ENSURING BLOOD SAFETY IN JAPAN","authors":"M. Shimizu, M. Takenaka","doi":"10.3925/jjtc1958.51.333","DOIUrl":"https://doi.org/10.3925/jjtc1958.51.333","url":null,"abstract":"HIV-infected voluntary blood donors have increased to more than 80 a year in the last 5 years. There are concerns that blood donation is being used for HIV testing by persons with high-risk behaviors, whose blood in the window period might result in HIV infection of recipients.A questionnaire was sent to 366 AIDS Care Core Hospitals, asking them to report the number of consultation by and characteristics of HIV-positive blood donors found by testing donations over the last 3 years. There were 185 HIV-positive donors reported, 158 (85%) of whom were male. Only 22 donors sought testing (12%), which increased to 13% over the last 5 years compared to 8% in previous years (not significant). However, 132 donors (71%) had high-risk behaviors including men who have sex with men (MSM: 57%) and multiple heterosexual partners (15%), but no intravenous drug users. Among the male donors, irrespective of whether they agreed to, denied or were undecided about donation for the purpose of HIV testing, MSM were the highest in each group (90%, 59%, and 77%, respectively), showing a remarkable increase to 71% over the last 5 years compared to 55% in 1993-1997 and 27% in 1986-1992. In addition, the fact that most infected donors did not approve of donation for the purpose of testing but revealed high-risk behavior once consulted a doctor in the hospitals shows a clear contradiction to their attitude at donation, at which time they ignored notices against donations for HIV testing or by those with high-risk behaviors before, at the time of, and after donation. Therefore, it should be considered that most but not all HIV-positive donors, particularly MSM, donated in order to obtain a HIV test.To ensure safety, it is recommended that MSM should be denied donating blood, and that donor recruitment should be focused on repeat donors, since HIV infection rates in first-time donors were three times higher than those in repeat donors.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"333-340"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEASURES TO INCREASE USE OF SCREENING TESTS FOR POST-TRANSFUSION INFECTION 采取措施增加使用输血后感染筛查试验

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.424

Mariko Kondo, M. Okamoto, Rieko Suzuki, M. Kurita, K. Horiba

{"title":"MEASURES TO INCREASE USE OF SCREENING TESTS FOR POST-TRANSFUSION INFECTION","authors":"Mariko Kondo, M. Okamoto, Rieko Suzuki, M. Kurita, K. Horiba","doi":"10.3925/JJTC1958.51.424","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.424","url":null,"abstract":"On July 30, 2003 the Revised Pharmaceutical Affairs Law and the Revised Transfusion Act were enacted by the Ministry of Health, Labor and Welfare to clarify the responsibilities of medical organizations. Taking this opportunity, the Hospital's Blood Transfusion Therapy Committee and Infection Control Committee undertook a review of methods of screening tests for post-transfusion infection. We decided to give patients a form letter to recommend they take a screening test for post-transfusion infection while providing notice to doctors that ‘2 months have passed since the transfusion (Blue Card)’. In addition, detailed discussions were held with respect to 1) test fees, 2) test criteria, 3) how to raise patients' awareness, and 4) how to enhance doctors' awareness. As a result, the use of screening tests for post-transfusion infection increased, from 6.8% to 54.6%, enabling us to confirm some effects. However, to fulfil our responsibilities to all patients receiving blood transfusion, the system for implementing this program requires urgent improvement. We thus aimed to further improve the system through operating the newly revised Blue Card system. In addition, it is necessary to create a system to ensure thorough implementation of the program with the electronic patient record due to commence in 2006.Meanwhile, the Ministry of Health, Labor and Welfare is expected to extend the application of patient assistance programs, in addition to implementing a system to ensure that patients receive safer blood transfusion.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"424-429"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

MULTI-DAY STORAGE OF PLATELETS AT ROOM TEMPERATURE IN A CONTAINER WITH HIGH OXYGEN PERMEABILITY 在高透氧性的容器中在室温下储存血小板多日

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/jjtc1958.51.578

S. Ezuki, Takatoshi Ito, Noriaki Shirahama, T. Takagi, K. Ikeda, S. Hashimoto, Kinuyo Kawabata, T. Ogata, and Hitoshi Ohto

{"title":"MULTI-DAY STORAGE OF PLATELETS AT ROOM TEMPERATURE IN A CONTAINER WITH HIGH OXYGEN PERMEABILITY","authors":"S. Ezuki, Takatoshi Ito, Noriaki Shirahama, T. Takagi, K. Ikeda, S. Hashimoto, Kinuyo Kawabata, T. Ogata, and Hitoshi Ohto","doi":"10.3925/jjtc1958.51.578","DOIUrl":"https://doi.org/10.3925/jjtc1958.51.578","url":null,"abstract":"The performance of platelet concentrates (PCs) for transfusion is greatly limited by their short shelf life. A highly oxygen-permeable container (PO-80, Kawasumi, Tokyo) was developed as an approach to extending the shelf life of PCs. Here, we compared PCs containing a high number of platelets (4 X 10\"/250mL plasma/bag) stored in a PO-80 container with those stored in a standard bag (control) (PL2410, Baxter Healthcare, USA) by monitoring platelet biochemical and functional parameters for up to 9 days. Three of six bags in the control bags had a pH below 6. 2 by day 7. These PCs also showed the disappearance of swirling, a rapid drop in glucose combined with a marked increase in lactic acid, accelerated P-selectin expression on platelets and an earlier decrease in both %HSR and aggregation of platelets in the observation period. In contrast, only one of six bags had a pH below 6. 2 by day 9 in the PO-80 container. Further, there was no disappearance of swirling. Other biochemical and functional parameters of PCs in PO-80 showed a slower change compared with those in the control bag. Overall, the in vitro characteristics of PCs showed a lower level of deterioration over 9 days when stored in a higher oxygen-permeable container than in a conventional bag. It is suggested that this newly developed highly oxygen-permeable PO-80 container will preserve adequate platelet function for longer storage periods and with higher PC yields.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3925/jjtc1958.51.578","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Analysis of Immunological Factors in Platelet Transfusion-Refractory Patients-Compatible Platelets to Improve Transfusion Efficacy 血小板输注难治性患者的免疫因素分析——配伍血小板提高输注疗效

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.520

S. Saito, Yumi Komatsu, S. Ota, K. Tamaki, Hideyuki Seshimo, Yuichiro Yamazaki, S. Nomura

{"title":"Analysis of Immunological Factors in Platelet Transfusion-Refractory Patients-Compatible Platelets to Improve Transfusion Efficacy","authors":"S. Saito, Yumi Komatsu, S. Ota, K. Tamaki, Hideyuki Seshimo, Yuichiro Yamazaki, S. Nomura","doi":"10.3925/JJTC1958.51.520","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.520","url":null,"abstract":"Refractoriness to platelet transfusions is considered one of the major obstacles in treating patients with hematological disorders. Although various transfusion procedures have been tried to prevent HLA alloimmunization, some patients still develop HLA and/or HPA antibodies and become refractory to platelet transfusions. We investigated the AHG-LCT, MPHA, LIFT and FlowPRA methods for detecting HLA antibodies and MPHA using chloroquine-treated platelets for HPA antibodies to analyze immunological factors in platelet transfusion-refractory (PTR) patients. Results showed that HLA antibodies were detected in 55 patients, HPA antibodies in 2 and both HLA and HPA antibodies in 2 patients. HLA-A and -B-compatible platelet transfusions were successful in boosting platelet levels in 27 of the patients. Nine patients developed not only HLA-A and/or -B antibodies but also HLA-C antibodies and became refractory to HLA-A and -B-compatible platelet transfusions. Matching of the HLA-C antigens was required in these patients to ensure the effectiveness of platelet transfusions. In some patients, ABH incompatibility reduced the effectiveness of transfusions. In these patients, platelet crossmatching was useful for predicting platelet survival. Of the 68 ABH-incompatible transfusions evaluated at 24 hours post-transfusion, 20 with positive crossmatches had corrected platelet count increments of 3.1±4.1 (×109)/L. In contrast, 48 transfusions with negative crossmatches had corrected platelet count increments of 18.1±7.6 (×109)/L. Other weak HLA antibodies in ten patients were not detected by the conventional methods but were detected by the FlowPRA method. Owing to screening and crossmatch tests, platelet transfusion efficacy was improved in 59 immunological PTR patients.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"24 1","pages":"520-529"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

SLOWLY PROGRESSIVE LUNG INJURY AFTER BLOOD TRANSFUSION: ASSOCIATION WITH BLOOD TRANSFUSION 输血后缓慢进行性肺损伤:与输血的关系

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.589

S. Matano, H. Iwasaki, A. Shirayama, Yuko Takahata, Yujin Tamura

{"title":"SLOWLY PROGRESSIVE LUNG INJURY AFTER BLOOD TRANSFUSION: ASSOCIATION WITH BLOOD TRANSFUSION","authors":"S. Matano, H. Iwasaki, A. Shirayama, Yuko Takahata, Yujin Tamura","doi":"10.3925/JJTC1958.51.589","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.589","url":null,"abstract":"An 82-year-old female was admitted to our hospital for treatment of a fracture of the femur. She demonstrated anemia and received red cell transfusion before and after surgery. Mild hypoxia was noted after the second red cell transfusion. At 72 hr after the first red cell transfusion (45 hr after the second), she developed marked hypoxia. She also complained of transient mild chest discomfort, but no clinical symptoms were reported at the time the marked hypoxia occurred. Bilateral pleural effusion and pulmonary edema were found on chest roentgenography. Acute myocardial infarction, cardiac failure, and pulmonary embolism were excluded by blood examination, ultrasonic cardiography, electrocardiography, and pulmonary perfusion scintigraphy, respectively ; however, hypoalbuminemia was found. She was administered oxygen, diuretics, and human serum albumin, after which pleural effusion, pulmonary edema, and hypoxia were resolved. Antigranulocyte antibodies or anti-HLA antibodies were not detected in sera from donors or recipient. The clinical features of this case were similar to those of transfusion-related acute lung injury, but, the course was slowly progressive.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"589-593"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70337138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ESTABLISHMENT OF THE FIRST NATIONAL STANDARD FOR NUCLEIC ACID AMPLIFICATION TECHNOLOGY ASSAY FOR HCV RNA 建立首个国家HCV RNA核酸扩增技术检测标准

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.515

S. Mizusawa, Y. Okada, Y. Horiuchi, Takeshi Tanaka, Koei Sato, Kenji Kaneko, Y. Sasaki, Toshiaki Tanaka, T. Tomono, Takeo Tomomizu, Shouichi Hayami, M. Hijikata, I. Hirako, M. Mayumi, Koichi Mikami, S. Mishiro, Seiji Miyamoto, Kengo Muta, K. Komuro, Teruhide Yamaguchi, T. Weimer, T. Gierman

引用次数: 3

PRODUCTION OF ANTI-D INDUCED BY RHD-NEGATIVE RED CELL TRANSFUSION IN A RHD-NEGATIVE PATIENT rh阴性患者输血诱导抗d的产生

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.585

S. Sakuma, K. Itou, Takayuki Suzuki, H. Yasuda, H. Ohto, Yayoi Muroi, Yoshinori Hirano, M. Kaneko

{"title":"PRODUCTION OF ANTI-D INDUCED BY RHD-NEGATIVE RED CELL TRANSFUSION IN A RHD-NEGATIVE PATIENT","authors":"S. Sakuma, K. Itou, Takayuki Suzuki, H. Yasuda, H. Ohto, Yayoi Muroi, Yoshinori Hirano, M. Kaneko","doi":"10.3925/JJTC1958.51.585","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.585","url":null,"abstract":"We experienced a case in which an anti-D antibody was developed after transfusion of RBCs from 19 apparently D-negative donors over a half-year period beginning in July 2000. The patient was 67-year-old. B-negative female with a history of sensitization by transfusion of D-positive RBCs. Antibody screening became positive and an anti-D was detected by indirect antiglobulin testing. However, no hemolytic episodes due to the anti-D were found in her clinical course. Specificity of the antibody was confirmed as an allo-anti-D by serological studies using differential absorption-elution or dithiothreitol-treated RBCs. We initially suspected the possibility of transfusion from D-positive or weak D donors and the Rh phenotypes of all 19 donors were back-checked. However, none was positive for D antigen by the indirect antiglobulin test and 5 donors included Ce haplotype. Genomic analysis to detect a Del, allele will demonstrate whether RBCs from a donor carrying a Del allele with Ce haplotype induced an allo-anti-D immune response.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"585-588"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

ANALYSIS OF WEAK REACTION SAMPLES IN REVERSED TYPING WITH COLUMN AGGLUTINATION TECHNOLOGY 反型弱反应样品的柱凝集分析

Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion Pub Date : 2005-01-01 DOI: 10.3925/JJTC1958.51.565

Yoko Hidaka, N. Kawata, M. Okuda, Tadashi Kato, Tomoko Kuribayashi, Nobuya Koyama, A. Ohara

{"title":"ANALYSIS OF WEAK REACTION SAMPLES IN REVERSED TYPING WITH COLUMN AGGLUTINATION TECHNOLOGY","authors":"Yoko Hidaka, N. Kawata, M. Okuda, Tadashi Kato, Tomoko Kuribayashi, Nobuya Koyama, A. Ohara","doi":"10.3925/JJTC1958.51.565","DOIUrl":"https://doi.org/10.3925/JJTC1958.51.565","url":null,"abstract":"The automated blood group testing system based on column agglutination technology(CAT) does not provide a standard for reversed typing. If the tube test(TT)method standard is introduced into automated the CAT method, the frequency of confirmation testing with the TT method will increase. In this study, we compared the results of the CAT and TT methods using samples which produced weak results with the automated CAT method(reactivity<3+;AutoVue System, Ortho). Forty-five percent of weak samples showed an increase in agglutination reactivity after a fixed 3-minute incubation time with the manual CAT method(Bio Vue, Ortho). All 481 weak samples had greater than 3+reactivity with the TT method. Eighty percent of weak samples possessed IgM antibody. Titration and scoring results with the TT method were greater than with the manual CAT method for all samples. Titration and scoring of the weak samples were low with all methods for all samples. In conclusion, we propose that 1+reactivity in the automated CAT method requires a confirmation test with the TT method because CAT 2+samples can present as 3+with the TT method.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"51 1","pages":"565-570"},"PeriodicalIF":0.0,"publicationDate":"2005-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2