RAPID QUANTITATION OF IgG ANTIBODIES SPECIFIC FOR BLOOD GROUP ANTIGENS A AND B BY SURFACE PLASMON RESONANSE

Abstract

The measurement of anti-blood group A/B (anti-A/B) IgG antibody levels is important for ABO unmatched-organ recipients because the effective removal of the antibodies improves their prognosis. However, currently existing methods to detect anti-A/B IgG antibodies suffer limitations due to high costs, low throughput, and poor adaptability to automation. We have developed a rapid means to quantitate anti-A/B IgG antibodies by surface plasmon resonance (SPR). The change in titers when the same plasma was diluted was precisely reflected by the SPR method. The coefficients of correlation between SPR and test tube (TT) methods for anti-A and -B IgG antibodies were 0.85 and 0.56, respectively. The SPR values also paralleled the TT values that showed a decline in titers after the removal of antibodies by double filtration plasma apheresis or plasma exchange.**This article is partly based on a study first reported in the Transfusion (Kimura, S., Yurugi, K., Yuasa, T., Tsuji, H., Segawa, H., Kuroda, J., Sato, K., Nogawa, M., Egawa, H., Tanaka, K., Maekawa, T.: Rapid quantitation of IgG antibodies specific for blood group antigens A and B by surface plasmon resonance. Transfusion, 45 (1): 56-62, 2005.)