T. Itoh, Hiromi Kanai, K. Takai, M. Hayashi, Y. Hoshi, H. Ohto
{"title":"THE PRESENT CONDITIONS AND PROBLEMS OF NURSES WORKING AT TRANSFUSION SECTIONS IN JAPANESE HOSPITALS : FOR THE ESTABLISHMENT OF A CERTIFIED TRANSFUSION NURSE","authors":"T. Itoh, Hiromi Kanai, K. Takai, M. Hayashi, Y. Hoshi, H. Ohto","doi":"10.3925/JJTC1958.50.596","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.596","url":null,"abstract":"Nurses play an important role in managing patients and donors at the time of transfusion blood collection, and are required to have advanced knowledge of transfusion medicine and technology. To identify the present conditions and problems in blood collection, particularly at the time of aphereis procedure in hospitals, we performed a questionnaire survey of nurses working at Japanese Transfusion Society-certified transfusion institutions. We also asked the nurses for opinions on the certified transfusion nurse system. The recovery rate of the questionnaire was 55%. The ratio of responses of nurses belonging to transfusion sections was 36%. The proportion of hospitals performing 10 or more of apheresis procedures per month was 10%, 6-10 times, 9%; 3-5 times, 30%; and 1-2 times, 51%. About 40% of nurses were uneasy about managing donors or patients during the apheresis procedure, mainly related to sudden change in patient condition or mechanical trouble. Inadequate management and knowledge were considered to be the causes of the unease. About 70% of nurses approved of the establishment of a certified transfusion nurse system. From these questionnaire findings, the nursing system in transfusion sections was judged inadequate, and improvement is required. We also detected the necessity for better education of nurses regarding the technology of blood transfusion medicine. For this purpose, an authorization system led by a society is considered to be the most effective. The Japan Society of Blood Transfusion and The Japanese Society for Apheresis are expected to take the lead towards realization of these goals.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"596-604"},"PeriodicalIF":0.0,"publicationDate":"2004-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ABO型 minor mismatch 生体肝移植後に抗B抗体が長期間遷延した1症例","authors":"忍 藤井, 正浩 桝屋, 由美 田中, 古川 美津子, 葛西 千枝子, 憲行 信田, 信行 南, 伸二 上本","doi":"10.3925/JJTC1958.50.626","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.626","url":null,"abstract":"We report a case of hemolytic anemia due to anti-B antibody after minor ABO-mismatched partial liver transplantation from a living donor. The patient was a 5-year-old girl with congenital biliary atresia, and group AB, Rh-positive blood. A partial liver transplant from her group A, D (Rh0) mother was performed. The recipient HLA was A2, 26; B54, 62; DR4, 15, and that of the donor was A2, -; B54, -; DR4, 14. This type of transplantation is the so-called one-way donor-recipient HLA matching. Her hemoglobin fell rapidly from 11.0g/dl to 6.0g/dl between days 10 and 15 after liver transplantation without evidence of bleeding. On day 14, anti-B IgM antibody was found in her serum. On day 16, two units of group O washed red blood cells were transfused, after which no further transfusions were required. This anti-B IgM antibody was thought to be produced from passenger B lymphocytes in the donor's liver. Although this type of antibody usually disappears within 4 weeks, it was continuously detected for about 4 months in this case. The long-term presence of anti-B antibody may be caused by one-way donor-recipient HLA matching.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"626-630"},"PeriodicalIF":0.0,"publicationDate":"2004-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"当院における「輸血検査24時間体制」の構築","authors":"古川 美津子, 篤芳 丸山, 葛西 千枝子, 由美 田中, 忍 藤井, 泰久 中川, 信行 南, 正浩 桝屋","doi":"10.3925/JJTC1958.50.436","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.436","url":null,"abstract":"One ABO-incompatible RBC unit was accidentally transfused to a patient in our hospital at midnight on October 22, 2000, at a time when serologic crossmatching for blood transfusion was not available in the Division of Blood Transfusion.We subsequently employed 4 part-time medical technologists at the Division of Blood Transfusion and, in cooperation with Central Clinical Laboratories, started a new system in which serologic crossmatching could be performed at anytime by medical technologists.Under this system, a total of 25 medical technologists are divided into two groups, A and B: Group A works from 5:00p.m, to 9:00p.m. on weekdays and from 8:30a.m. to 5:00p.m. on weekends or holidays; Group B works during the other hours. The duties of Group A are 1) ABO & Rh typing, 2) crossmatching, 3) management of blood components and 4) irradiation of blood components. The duties of Group B are 1) ABO & Rh typing and 2) crossmatching.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"436-443"},"PeriodicalIF":0.0,"publicationDate":"2004-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Anan, M. Okubo, A. Yagishita, H. Ohki, A. Imai, Keiko Watanabe, Y. Kuboi, T. Shimada, Eriko Mori, R. Hirata, H. Maeda
{"title":"EFFECTS OF PRESTORAGE LEUKOCYTE REDUCTION IN ENDOTOXIN-CONTAMINATED AUTOLOGOUS BLOOD","authors":"M. Anan, M. Okubo, A. Yagishita, H. Ohki, A. Imai, Keiko Watanabe, Y. Kuboi, T. Shimada, Eriko Mori, R. Hirata, H. Maeda","doi":"10.3925/JJTC1958.50.581","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.581","url":null,"abstract":"To clarify the effectiveness of prestorage leukocyte reduction for whole blood contaminated with endotoxin, we analyzed biochemical mediators, levels of cytokines, and aggregates in blood samples on storage for five weeks.From 12 healthy donors, 400mL of blood were collected into CPDA-1 bags as autologous blood samples. A standard E. coli-derived endotoxin (500pg/mL) was pulsed into all of the bags. Half of each blood sample was filtered to eliminate leukocytes. We assayed pH, Na, K, ATP, free Hb, 2, 3-DPG, LDH, bradykinin, C3a, aggregates, RANTES, granulocyte elastase, IFN-γ, IL-1β, TNF-α, and IL-6 at 3 hours and on days 7, 14, 21, and 35.There was no adverse effect of leukocyte reduction on erythrocytes. Levels of IL-1β and IL-6 in the leukocyte-reduced blood were significantly lower than those in blood without reduction. It was confirmed that the number of aggregates was decreased by filtration for leukocyte reduction.These results suggest that prestorage leukocyte reduction to reduce the risk of endotoxin-related adverse reactions is feasible for storage of autologous blood bags for five weeks, especially for patients with jaundice.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"581-587"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Saito, M. Ota, Y. Katsuyama, H. Makishima, T. Tamai, K. Takayanagi, H. Asamura, H. Fukushima
{"title":"SENSITIVITY OF VARIOUS METHODS FOR DETECTING HLA CLASS I ANTIBODIES AND SCREENING FOR ALLOANTIBODIES IN 145 SERA SAMPLES FROM PATIENTS RECEIVING PLATELET TRANSFUSIONS:A COMPARISON OF AHG‑LCT LIFT PIFT MAGNETIC MPHA AND FlowPRA METHODS","authors":"S. Saito, M. Ota, Y. Katsuyama, H. Makishima, T. Tamai, K. Takayanagi, H. Asamura, H. Fukushima","doi":"10.3925/JJTC1958.50.753","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.753","url":null,"abstract":"A total of 15 distinct human sera that did not exhibit reactivity against HPA antibodies but contained HLA-specific antibodies were used for sensitivity analysis of the AHG-LCT, LIFT, PIFT, M-MPHA and FlowPRA methods. The HLA antibody titer of each serum sample was defined as the highest dilution of serum that became positive to each method. 145 sera samples from patients receiving platelet transfusions were also screened to detect HLA antibodies by these methods. Results showed that FlowPRA was the most sensitive for identifying HLA antibodies. Although FCM is generally considered the most sensitive method, our analysis suggests that M-MPHA is as useful as FCM in both sensitivity and screening tests. PIFT sensitivity was slightly less than that of LIFT and M-MPHA. AHG-LCT sensitivity was significantly less than that of the other methods.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"753-760"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Ishimaru, C. Tenman, Y. Fujiwara, M. Ohe, T. Kato, H. Ikeda
{"title":"A CASE OF DELAYED HEMOLYTIC TRANSFUSION REACTION PROBABLY CAUSED BY A PRIMARY IMMUNE RESPONSE","authors":"K. Ishimaru, C. Tenman, Y. Fujiwara, M. Ohe, T. Kato, H. Ikeda","doi":"10.3925/JJTC1958.50.768","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.768","url":null,"abstract":"We report here a rare case of delayed hemolytic transfusion reaction (DHTR) probably caused by a primary immune response. The patient was a 32-year-old Japanese female who underwent surgery for ectopic pregnancy. For treatment of intraoperative hemorrhage shock, she received 5 units of RBCs (all positive for Jka, C and e antigen) that were crossmatch-compatible by indirect antiglobulin testing using polyethylene glycol (PEG-TAT). On day 22 after transfusion, she showed symptoms of hemoglobinuria, reticulocytosis, and elevated serum levels of LDH (1,106IU/L) and total-bilirubin (5.6mg/dL). She did not receive further transfusion during this period. On irregular antibody screening of patient serum collected 24 days after transfusion, anti-Jka was detected by PEG-TAT. Her blood was typed as B, DccEE, Jk (a-b+). She then received 1 unit of RBCs (Jka-negative but C, e-positive). Her anemia improved after transfusion and no hemolytic transfusion reaction was observed. IgM type anti-C + e in the patient detected on day 24 after the first transfusion did not seem to be involved in the DHTR because transfusion of C + e + RBCs on the 24th day did not cause DHTR. Further, we demonstrated that the immunoglobulin (Ig) class of the anti-Jka switched from IgM to IgG during the clinical course. These results suggest that the DHTR was probably due to anti-Jka caused by a primary immune response.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"768-773"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70336206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"THE RhDel ALLELE IN THE JAPANESE POPULATION","authors":"Y. Ishikawa, H. Tsuneyama, M. Uchikawa, M. Satake","doi":"10.3925/JJTC1958.50.710","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.710","url":null,"abstract":"The RHD gene of D-negative Japanese individuals was analyzed to determine the causes of the D-negative phenotype. cDNAs prepared from reticulocytes were used for the sequencing of full length RH transcripts. Although the complete RHCE sequence was obtained from the cDNA clones of D-negative and RHD gene-positive (D-RHD +) individuals, the complete sequence of RHD was not. The longest sequence derived from RHD had exon 9 deleted. A synonymous one-point mutation, G1227A, at the 3′-end of exon 9 was found in the genomic DNA. The allele was shared by more than 90% (28/31) of D-RHD +individuals, most of whom had the Del phenotype. To distinguish the Del, allele from D-positive alleles, a multiplex PCR-SSP (sequence specific primer) method was designed.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"710-713"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Moriyama, M. Satake, R. Yasunaga, T. Yuasa, Yasuyuki Suzuki, K. Ishii
{"title":"EVALUATION OF QUALITIES ON A CLOSED-BAG SYSTEM WITH WHOLE BLOOD LEUKOCYTE FILTER (WBF2) FOR PRE-STORAGE LEUKOREDUCTION","authors":"S. Moriyama, M. Satake, R. Yasunaga, T. Yuasa, Yasuyuki Suzuki, K. Ishii","doi":"10.3925/jjtc1958.50.34","DOIUrl":"https://doi.org/10.3925/jjtc1958.50.34","url":null,"abstract":"In order to avoid a wide variety of side effects associated with blood transfusion such as nonhemolytic febrile transfusion reactions (NHFTR), HLA-sensitization and Cytomegalovirus infection. Leukocyte filters have come into wide use in bedside blood transfusion in Japan. During the storage of blood components, however, residual leukocytes disintegrate and release various biologically active substances, which constitute one of the causes of transfusion-related adverse events. Therefore, removal of leukocytes immediately or at an early stage after blood collection is expected to decrease the incidence of transfusion-related side effects by controlling the deterioration of blood cells or preventing the accumulation of cytokines.The WBF2 closed-bag system is a blood collection-component separation system designed for the removal of leukocytes from whole blood and the preparation of blood components by centrifugation. In the present study, we evaluated the usefulness of the system in the preparation of leukocyte-reduced RC-MAP and FFP using WBF2, and compared the quality of these preparations with those by conventional post-storage leukoreduction or non-leukoreduction. The WBF2 system yielded red cell components with superior red cell recovery and lower accumulation of some cytokines, demonstrating its suitability for use in a blood center program. It was also noted that there was almost no macroaggregation in red cell components prepared by prestorage leukoreduction.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"34-55"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Furuta, Naomi Higashi, Yoshihisa Watanabe, K. Isa, E. Shimada, K. Ikeda
{"title":"A CASE OF TRANSFUSION REACTION ASSOCIATED WITH ANTI-IgA ANTIBODY","authors":"Y. Furuta, Naomi Higashi, Yoshihisa Watanabe, K. Isa, E. Shimada, K. Ikeda","doi":"10.3925/JJTC1958.50.419","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.419","url":null,"abstract":"We report the case of a patient with systemic lupus erythematosus with selective IgA deficiency who developed anaphylactic transfusion reactions. A 50-year-old woman with systemic lupus erythematosus was suspected of having IgA deficiency due to IgA nondetection in the immunoglobulin test on admission, but the test for anti-IgA antibody was not performed. She was administered a platelet concentrate transfusion because of prolonged bleeding time after surgery. About 10min after starting transfusion, she developed palpitations, dyspnea, and hypotension, and went into shock. Anti-IgA antibody was detected from the patient's stored serum collected before the transfusion. It was suggested that IgA present in the transfused platelet concentrate may have induced the anaphylactic shock after reacting with the pre-existing anti-IgA antibody. To avoid unexpected anaphylactic transfusion reactions, attention should be paid to the presence of anti-IgA antibody in patients with selective IgA deficiency.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"419-424"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Nibu, Yuriko Nomaguchi, K. Kubota, Y. Yoshiura, Midori Kumagawa, M. Naito
{"title":"BLOOD DONATION FOR AUTOLOGOUS USE BY ORTHOPEDIC PATIENTS WITHOUT THE USE OF RECOMBINANT ERYTHROPOIETIN","authors":"K. Nibu, Yuriko Nomaguchi, K. Kubota, Y. Yoshiura, Midori Kumagawa, M. Naito","doi":"10.3925/JJTC1958.50.693","DOIUrl":"https://doi.org/10.3925/JJTC1958.50.693","url":null,"abstract":"We analyzed the use of recombinant erythropoietin (r-EPO) among 368 patients, each of whom had donated more than 800ml for later autologous transfusion during orthopedic surgery. Of the 269 patients who would have required r-EPO according to the health insurance authority guideline, 259 patients were able to donate blood without using r-EPO with no observed adverse effects, despite a significantly lower average Hb level (12.8±1.1g/dl) than the 99 patients who did not require r-EPO according to that guideline (14.8±0.7g/dl). The remaining 10 patients had Hb levels less than 11g/dL at the start of the blood donation and were given r-EPO. Analysis of the data of the two groups which did not use r-EPO revealed that there was no statistically significant difference in the mean total volume of donated blood nor in the frequency of required allogenic blood, but that the mean donation period was significantly longer in the 259 patients at 19.4±12.1 days vs 15.5±8.8 days. These findings therefore indicate that most orthopedic patients who need 800ml of blood during surgery can safely donate this amount of autologous blood without using r-EPO if they start donation 30 days or more prior to surgery.","PeriodicalId":86521,"journal":{"name":"Nihon Yuketsu Gakkai zasshi = Journal of the Japan Society of Blood Transfusion","volume":"50 1","pages":"693-698"},"PeriodicalIF":0.0,"publicationDate":"2004-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70335557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}