Asia Oceania Journal of Nuclear Medicine and Biology最新文献

{"title":"Improvement of image quality for small lesion sizes in ¹⁸F-FDG prone breast silicon photomultiplier-based PET/CT imaging.","authors":"Nobuhiro Yada, Hiroyuki Kuroda, Toshihiko Kawamura, Mizuki Fukuda, Yoshinori Miyahara, Takeshi Yoshizako, Yasushi Kaji","doi":"10.22038/aojnmb.2024.78080.1553","DOIUrl":"10.22038/aojnmb.2024.78080.1553","url":null,"abstract":"<p><strong>Objectives: </strong>We investigated image quality and standardized uptake values (SUVs) for different lesion sizes using clinical data generated by ¹⁸F-FDG-prone breast silicon photomultiplier (SiPM)-based positron emission tomography/computed tomography (PET/CT).</p><p><strong>Methods: </strong>We evaluated the effect of point-spread function (PSF) modeling and Gaussian filtering (Gau) and determined the optimal reconstruction conditions. We compared the signal-to-noise ratio (SNR), contrast, %coefficient of variation (%CV), SUV, and Likert scale score between ordered-subset expectation maximization (OSEM) time-of-flight (TOF) and OSEM+TOF+PSF in phantom and clinical studies. The conventional image was generated with OSEM+TOF_Gau 6 mm. The National Electrical Manufacturers Association body phantom with 10-mm hot sphere data was acquired for 5 min. Twenty-six patients (40 lesions, ranging from 3.7 to 63.0 mm) were examined using prone breast PET/CT with a breast positioner for breast cancer staging. PET data were acquired 125±9.7 min after intravenous injection of 220±16.1 MBq at 5 min/bed.</p><p><strong>Results: </strong>In the phantom study, a high SNR was obtained from a 3- to 5-mm Gaussian filter for OSEM+TOF+PSF. The contrast obtained with OSEM+TOF without Gaussian filtering was superior to that obtained with OSEM+TOF+PSF_Gau 4 mm. In the clinical study, the image quality depended on lesion size. The average SNR was significantly higher at 40.8% for lesions >20 mm with OSEM+TOF_Gau 6 mm than with OSEM+TOF without Gaussian filtering. The average contrast for lesions ≤10 mm was significantly higher by 42.0% with OSEM+TOF without Gaussian filtering than with OSEM+TOF_Gau 6 mm. The average SUV_max of OSEM+TOF without Gaussian filtering significantly increased by 53.3% for lesions ≤10 mm.</p><p><strong>Conclusion: </strong>OSEM+TOF without Gaussian filtering provided good contrast and quantitative value for small lesions.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"13 1","pages":"77-86"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682473/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of the specific binding ratio distribution to characterise multiple system atrophy in advanced iodine-123-labelled N-(3-fluoropropyl)-2β-carbomethoxy-3β-(4-iodophenyl) nortropane serotonin transporter imaging.","authors":"Kazuya Takahashi, Masanobu Ishiguro, Yoshitaka Inui, Takashi Ichihara, Cong Shang, Ryunosuke Nagao, Yasuaki Mizutani, Mizuki Ito, Hirohisa Watanabe, Nobutoku Motomura, Hiroshi Toyama","doi":"10.22038/aojnmb.2024.78274.1557","DOIUrl":"10.22038/aojnmb.2024.78274.1557","url":null,"abstract":"<p><strong>Objectives: </strong>Sudden death in multiple system atrophy (MSA) is caused by decreased serotonergic innervation, but there is no routine test method for this decrease. In addition to dopamine transporters, iodine-123-labelled N-(3-fluoropropyl)-2β-carbomethoxy-3β-(4-iodophenyl) nortropane (¹²³I-FP-CIT) binds serotonin transporters (SERTs). We noted a binding potential to quantify the total quantity of ¹²³I-FP-CIT binding to its receptors.Following Mintun's binding-potential concept, this study aimed to evaluate the relationship between the specific binding ratio (SBR) and total SERT tissue amount, but not SERT binding, and to develop an SBR imaging method to measure brain-stem SERT. We sought to establish a binding-potential imaging procedure using SBR images to examine differences in the brain-stem SERT distribution between healthy subjects and MSA patients.</p><p><strong>Methods: </strong>Single-photon emission computed tomography (SPECT) and T1-weighted magnetic resonance (MR) images were aligned. The MR (T1) images were used to set a reference site for the occipital-lobe SBR in each subject, and measurements were made from the SPECT image at the same position. The pixel values and accumulation ratios compared with the occipital lobe were calculated, and a regional SBR distribution image was created. We identified areas with SERT accumulation above a certain level.</p><p><strong>Results: </strong>The SERT accumulation site was visualised as an SBR value on MR images. The accumulation distribution (SERT distribution) on the SBR images significantly differed between the healthy subjects and patients with MSA.</p><p><strong>Conclusion: </strong>SERT accumulation was noted in the brain-stem region, indicating that SBR imaging was useful for viewing and quantifying SERT accumulation.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"13 1","pages":"33-41"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682478/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913773","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ventilation/Perfusion Mismatch in Pulmonary Vein Stenosis Secondary to Atrial Fibrillation Ablation.","authors":"Leah Anne Christine L Bollos, Ryosuke Kasai, Hideki Otsuka, Yoichi Otomi, Tomomi Matsuura, Tamaki Otani, Koji Yamaguchi, Takanori Bando, Yuya Ueki, Noritake Matsuda, Satoru Takashi, Shota Azane, Yamato Kunikane, Shoichiro Takao, Shusuke Yagi, Masataka Sata, Hitoshi Ikushima, Masafumi Harada","doi":"10.22038/aojnmb.2024.79650.1561","DOIUrl":"10.22038/aojnmb.2024.79650.1561","url":null,"abstract":"<p><p>We present two patients with a history of paroxysmal atrial fibrillation who developed pulmonary vein stenosis (PVS) following atrial fibrillation (AF) ablation. Case 1 involved a female patient in her 50s who was asymptomatic for pulmonary symptoms but was found to have a high degree of left superior PVS 15 months after AF ablation. This was demonstrated using contrast-enhanced computed tomography (CE-CT) and supported by findings of perfusion defects on ventilation-perfusion (V/Q) scan. Case 2 was a male patient in his 60s who developed progressive left superior PVS nine months after AF ablation, evidenced by serial CE-CT and V/Q scans. PVS is a rare but well-known complication of pulmonary vein ablation for the treatment of AF that can lead to severe complications if left untreated. V/Q scans effectively assess the functional significance of PVS by detecting abnormal blood flow segments. Although a V/Q mismatch characterized by reduced perfusion defects is more commonly used in evaluating pulmonary embolism, PVS should not be disregarded as a differential diagnosis. Few studies emphasize the utility of V/Q scans in managing PVS and highlight V/Q mismatch as a notable finding. This case report aimed to highlight their significance.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"13 1","pages":"62-69"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682475/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"¹⁸F-FDG PET/CT in lymphangiosarcoma: A case report and review of literature.","authors":"Nitin Gupta","doi":"10.22038/aojnmb.2024.77689.1548","DOIUrl":"10.22038/aojnmb.2024.77689.1548","url":null,"abstract":"<p><strong>Objectives: </strong>Lymphangiosarcoma is a rare tumor that affects the upper limbs of patients who have undergone breast cancer surgery, including axillary dissection, followed by radiation therapy (RT) to the axilla and has a poor prognosis. ¹⁸F-FDG PET/CT may enable the earlier detection of malignant transformation in a setting of chronic lymphedema and help evaluate the extent and staging of the tumor, allowing earlier initiation of treatment options.</p><p><strong>Case presentation: </strong>We herein report a case of cutaneous lymphangiosarcoma in a 47-year-old breast carcinoma patient, which occurred 9 years after initial surgery and radiation therapy. Distant metastases were detected on ¹⁸F-FDG PET/CT. The patient underwent fore-quarter amputation of the upper limb and concurrent chemo-radiation therapy. However, she succumbed to her disease after 3 cycles of chemotherapy.</p><p><strong>Conclusions: </strong>¹⁸F-FDG PET/CT scan helps in the early detection of malignant transformation and lymphangiosarcoma in a setting of chronic lymphedema in breast carcinoma patients following radiation therapy to the axilla. Furthermore, it helps determine the extent of regional spread and detect metastatic involvement, thus enabling better clinical management of these patients.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"13 1","pages":"107-113"},"PeriodicalIF":0.0,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11682481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142913763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-distribution study of Tc-99m HMPAO labeled platelet in healthy volunteer.","authors":"Mahdieh Parvizi, Mehrshad Abbasi, Hojjat Ahmadzadehfar, Abbas Tafakhori, Maryam Naseri, Ali Khalaj, Saeed Farzanehfar","doi":"10.22038/AOJNMB.2024.71620.1525","DOIUrl":"https://doi.org/10.22038/AOJNMB.2024.71620.1525","url":null,"abstract":"<p><strong>Objectives: </strong>The bio-distribution of Tc-99m HMPAO labeled platelets (LP), which could be used to image subtle thrombosis, is not reported in a human yet, which is the subject of the current study.</p><p><strong>Method: </strong>The platelets were extracted from 49 ml whole blood and labeled with Tc-99m HMPAO, then re-injected to the healthy volunteer. Anterior and posterior whole body imaging was done by a dual-head gamma camera 3, 18, 33, 46, 81, 124, 190 min and 15 hours after injection. Also a whole-body SPECT was done at 137 min post-injection. The area under the curves of the spleen, liver, left kidney, bladder, right lung, brain, and abdominal aorta ROIs was calculated to estimate the accumulation of labeled platelets within the organs.</p><p><strong>Results: </strong>The spleen was the target organ. The kidneys, liver, and heart were also remarkably visualized. The thyroid, stomach, bladder, or gastrointestinal (GI) uptake/activity was not significant. The stomach visualization was enhanced after ingestion at 60 min. The sagittal and lateral sinuses were delineated, and the background of the brain was very low. During the study, the area under the curve of activity was 738, 308, 302, 196, 230, 121, 79, 216, 529, 369, 162, and 54 counts. min/pixel for spleen, liver, heart, right lung, left kidney, right iliac artery, sagittal sinus, thyroid, bladder, stomach, GI, and background, respectively.</p><p><strong>Conclusion: </strong>The quality of the scan with low dose Tc-99m HMPAO LPs is optimal. We documented the bio-distribution of LPs. The optimal imaging time was 80-120 min post-injection when the free Tc-99m and GI transit were negligible. The sagittal and lateral sinuses were visualized enabling detection of possible clots in the vessels.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"12 2","pages":"142-148"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11263780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Incidental Finding of Triple Ectopic Thyroid: Case Report and Review of the Literature.","authors":"Fatima Zeineddine, Gerard El-Hajj, Marie-Ange Hajj, Rita Chahinian","doi":"10.22038/AOJNMB.2024.75895.1532","DOIUrl":"https://doi.org/10.22038/AOJNMB.2024.75895.1532","url":null,"abstract":"<p><p>Ectopic thyroid tissue is a rare congenital anomaly, with the presence of three simultaneous ectopic foci being exceedingly rare. We describe a case of a totally asymptomatic 26-year-old male discovered to have triple ectopic thyroid following incidental elevated thyroid-stimulating hormone (TSH) levels. Subsequent ultrasonography of the neck showed an absent thyroid gland in its conventional location. A Technetium-99m pertechnetate (Tc-99m) thyroid scan showed three distinct foci of radiotracer uptake in the upper cervical, lingual, and sublingual regions, corresponding to triple ectopic thyroid. An extensive review of the literature was conducted to provide a broader understanding and deeper insights into this uncommon condition. This case underscores the pivotal role of Technetium-99m thyroid scanning in diagnosing triple ectopic thyroid, particularly in instances where the orthotopic thyroid gland is absent. A comprehensive understanding of this rare entity is indispensable for radiologists and clinicians, enabling accurate diagnosis and informed decision-making regarding the appropriate therapeutic strategies.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"12 2","pages":"179-184"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11263775/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"¹⁸F-FDG PET/CT imaging of IgG4-producing MALT lymphoma with multiple site involvement.","authors":"Kodai Kawaji, Seiji Kurata, Katsuhisa Matsuo, Hiroaki Miyoshi, Jun Akiba, Fumihiko Mouri, Akiko Sumi, Kiminori Fujimoto, Toshi Abe","doi":"10.22038/AOJNMB.2023.73477.1512","DOIUrl":"10.22038/AOJNMB.2023.73477.1512","url":null,"abstract":"<p><p>¹⁸F-FDG PET/CT is regarded as a modality utilized for the purpose of lesion localization, staging and assessment of treatment response in patients with lymphoma. However, it is difficult that we diagnose among multifocal lymphoma, IgG4-related disease (IgG4-RD), or a combination of both conditions when confronted with multiple sites of ¹⁸F-FDG uptake with heightened serum IgG4 levels. We present a case of a 72-year-old male who was suspected of Sjögren's syndrome based on symptoms of xerostomia accompanied by swelling of the bilateral upper eyelid and salivary glands. Following a diagnostic biopsy that revealed mucosa-associated lymphoid tissue (MALT) lymphoma as a possible finding, ¹⁸F-FDG PET/CT was conducted, which demonstrated multiple sites of ¹⁸F-FDG accumulation. While multifocal MALT lymphoma was initially suspected, the coexistence of IgG4-RD could not be definitively ruled out due to the elevated serum IgG4 levels. Subsequent histopathological and immunohistochemical examinations confirmed the diagnosis of IgG4-producing MALT lymphoma. After receiving systemic therapy with rituximab, the swelling of the bilateral upper eyelid and parotid glands resolved upon visual examination, and the serum IgG4 levels returned to within the normal range in a few months. No new lesions were detected during the subsequent follow-up examinations conducted over a period of 3 years.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"12 1","pages":"52-56"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Formulating a national position statement and guide on modern theranostics in the Philippines.","authors":"Patricia A Bautista-Peñalosa, Francis Gerard M Estrada, Emerita A Barrenechea, Teofilo O L San Luis","doi":"10.22038/AOJNMB.2023.72838.1508","DOIUrl":"10.22038/AOJNMB.2023.72838.1508","url":null,"abstract":"<p><p>Barriers to the establishment of advanced technologies in developing countries were overcome when modern theranostics pertaining to the use of Ga-68 and Lu-177 PSMA and DOTATATE were first offered to patients in the Philippines in early 2018. However, significant growth was not experienced at St. Luke's Medical Center for five years and lutetium was not yet distributed to other institutions by a radiopharmaceutical supplier. Due to the relative novelty and rapid expansion of theranostics worldwide, position statements were released by the Australasian Association of Nuclear Medicine Specialists, European Association of Nuclear Medicine, Society of Nuclear Medicine and Molecular Imaging, and International Atomic Energy Agency primarily to uphold patient safety and ensure a level of standard among its practitioners. Subsequently in the latter half of 2022, these were adopted and modified according to what is feasible and applicable locally within the Philippine Society of Nuclear Medicine, considering the current status and future possibilities. Different representatives were involved, and several groups were mobilized for successful implementation. A liability clause was incorporated to discourage unprofessional acts.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"12 1","pages":"69-72"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757056/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the diagnostic value of Sentinel Lymph Node in patients with gastric adenocarcinoma.","authors":"Ramin Sadeghi, Reza Taheri, Ali Jangjoo, Akbar Pakdel, Mohammad-Hassan Arjmand, Mohammad Reza Motiei, Bahram Memar, Mohsen Aliakbarian","doi":"10.22038/AOJNMB.2023.70461.1491","DOIUrl":"10.22038/AOJNMB.2023.70461.1491","url":null,"abstract":"<p><strong>Objectives: </strong>Sentinel lymph node biopsy (SLNB) has been proven as a safe and efficient procedure in some cancers like breast cancer and melanoma with a reduction of complications and side effects of unnecessary lymphadenectomy in many patients. However, the diagnostic value of SLNB in gastric cancer is a point of debate. This study evaluated the diagnostic value of SLNB using radiotracer and isosulphan blue dye injection in patients with Gastric Adenocarcinomas (GA).</p><p><strong>Methods: </strong>This descriptive study was performed at Imam-Reza HOSPITAL on 39 patients diagnosed with GA with no lymphatic metastasis using two methods: the combination of radionuclide with isosulphan together (R&I) method compared with the isosulphan alone method. Lymphatic dissection was performed in all patients. The pathological results were compared between the sentinel lymph nodes (SLN) and other lymph nodes and their accordance rate was calculated.</p><p><strong>Results: </strong>In the T1 group, the sentinel lymph node biopsy detection rate was 100% for the combination of the R&I method and 60% for the isosulphan method and the false negative rate was zero. These values respectively were 88.8% and 88.8% in the T2 group with a false negative rate of 75%. In the T3 group, the values were 100% for the combination of the R&I method and 93.7% for the isosulphan method with a false negative rate of 40%. In the combination of the R&I method, the sensitivity, specificity, and positive and negative predictive values were 57.9, 100, 100, and 69.2 percent respectively.</p><p><strong>Conclusion: </strong>Based on the false negative rate (47.4%), SLNB by injection of isosulphan blue dye alone is not a diagnostic enough value for predicting lymph node metastasis in GA. Although, SLNB by combination of the R&I had better accuracy compared to the isosulphan alone, more studies with larger samples are needed to prove this result.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"12 1","pages":"21-26"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10757058/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139073282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the Optimization of the Protocol for Automated Radiosyntheses of [⁶⁸Ga]Ga-Pentixafor, [⁶⁸Ga]Ga-FAPI-4 and [⁶⁸Ga]Ga-DOTATATE in a Modular-Lab Standard.","authors":"Sreeja Raj Menon, Arpit Mitra, Sudeep Sahu, Sangita Lad, Avik Chakraborty, Mukti Kanta Ray, Sharmila Banerjee","doi":"10.22038/AOJNMB.2024.77059.1545","DOIUrl":"https://doi.org/10.22038/AOJNMB.2024.77059.1545","url":null,"abstract":"<p><strong>Objectives: </strong>The present work describes the automated radiochemical synthesis of different PET tracers like [⁶⁸Ga]Ga-Pentixafor, [⁶⁸Ga]Ga-FAPI-4 and [⁶⁸Ga]Ga-DOTATATE using optimized single protocol in the non-cassette based Eckert & Ziegler (EZ) Modular Lab (fixed tubing system) without any modification in the inbuilt human machine interface (HMI) software. Recently, PET agents viz. [⁶⁸Ga]Ga-Pentixafor and [⁶⁸Ga]Ga-FAPI-4 are gaining prominence for the diagnosis of overexpressed Chemokine Receptor-4 (CXCR4) and Fibroblast Activation Protein (FAP) receptor, respectively, in the microenvironment of numerous cancer types. The promising results observed with the clinical usage of [⁶⁸Ga]Ga-DOTATATE produced using the automated protocol, provided impetus for the clinical translation of [⁶⁸Ga]Ga-Pentixafor and [⁶⁸Ga]Ga-FAPI-4 using the in-house developed automated radiolabeling protocol.</p><p><strong>Methods: </strong>Herein we report a single radiolabeling protocol for the automated preparation of [⁶⁸Ga]Ga-Pentixafor and [⁶⁸Ga]Ga-FAPI-4 in the non-cassette based EZ Modular-Lab Standard radiochemistry module, without any changes in schematic, graphical user interface (GUI) software and time list, from that used for routine production of [⁶⁸Ga]Ga-DOTATATE in our centre, since 2015. Physico-chemical quality control and in-vitro stability analyses were carried out using radio-TLC and radio-HPLC.</p><p><strong>Results: </strong>The automated protocol yielded reliable and consistent non-decay corrected (ndc) radiochemical yield (RCY) of (84.4%±0.9%) and (85.5%±1.4%) respectively, for [⁶⁸Ga]Ga-Pentixafor and [⁶⁸Ga]Ga-FAPI-4, with RCP>98%, which are comparable to the RCY of (84.4%±1.2%) and RCP (99.1%±0.3%) for [⁶⁸Ga]Ga-DOTATATE. The biological quality control studies confirmed the formulations to be of ready-to-use pharmaceutical grade.</p><p><strong>Conclusion: </strong>The consistent and reliable RCY and RCP of multiple ⁶⁸Ga-labeled PET tracers by single automated radiochemistry protocol exhibits the versatility of the EZ Modular Lab.</p>","PeriodicalId":8503,"journal":{"name":"Asia Oceania Journal of Nuclear Medicine and Biology","volume":"12 2","pages":"149-160"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11263773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141756877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}