Asian Biomedicine最新文献_第2页

Bedside clinical assessment of patients with common upper limb tremor and algorithmic approach 常见上肢震颤患者的床边临床评估和算法方法

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-05-02 DOI: 10.2478/abm-2024-0008

Pattamon Panyakaew, Warongporn Phuenpathom, Roongroj Bhidayasiri, Mark Hallett

{"title":"Bedside clinical assessment of patients with common upper limb tremor and algorithmic approach","authors":"Pattamon Panyakaew, Warongporn Phuenpathom, Roongroj Bhidayasiri, Mark Hallett","doi":"10.2478/abm-2024-0008","DOIUrl":"https://doi.org/10.2478/abm-2024-0008","url":null,"abstract":"The diagnostic approach for patients with tremor is challenging due to the complex and overlapping phenotypes among tremor syndromes. The first step in the evaluation of tremor is to identify the tremulous movement and exclude the tremor mimics. The second step is to classify the tremor syndrome based on the characteristics of tremor from historical clues and focused examination (Axis 1). Comprehensive tremor examinations involve the assessment of tremor in different conditions (rest, action or mixed, position or task-specific), distribution of tremor (upper limb, lower limb, head, jaw), positive signs for functional tremor (FT) if suspected (distractibility, entrainment, co-contraction), and associated neurological signs including parkinsonism, dystonic posture, cerebellar/brainstem signs, neuropathy, and cognitive impairment. A pivotal feature in this step is to determine any distinct feature of a specific isolated or combined tremor syndrome. In this review, we propose an algorithm to assess upper limb tremors. Ancillary testing should be performed if clinical evaluation is unclear. The choice of investigation depends on the types of tremors considered to narrow down the spectrum of etiology (Axis 2). Laboratory blood tests are considered for acute onset and acute worsening of tremors, while structural neuroimaging is indicated in unilateral tremors with acute onset, nonclassical presentations, and a combination of neurological symptoms. Neurophysiological study is an important tool that aids in distinguishing between tremor and myoclonus, etiology of tremor and document specific signs of FT. Treatment is mainly symptomatic based depending on the etiology of the tremor and the patient’s disabilities.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140834821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elevated level of neuroserpin is an indication for the resistance to gambogic acid-induced apoptosis and oxidative stress in triple-negative breast cancer cells 神经肽水平升高表明三阴性乳腺癌细胞对甘草酸诱导的细胞凋亡和氧化应激具有抵抗力

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-05-02 DOI: 10.2478/abm-2024-0010

Ertan Kucuksayan, Hakan Kucuksayan, Mehmet Enes Sozen, Aslinur Sircan-Kucuksayan

{"title":"Elevated level of neuroserpin is an indication for the resistance to gambogic acid-induced apoptosis and oxidative stress in triple-negative breast cancer cells","authors":"Ertan Kucuksayan, Hakan Kucuksayan, Mehmet Enes Sozen, Aslinur Sircan-Kucuksayan","doi":"10.2478/abm-2024-0010","DOIUrl":"https://doi.org/10.2478/abm-2024-0010","url":null,"abstract":"Background The triple-negative breast cancer (TNBC) subtype, characterized by loss of HER2, estrogen, and progesterone receptors, displays aggressive phenotype and poor prognosis compared to other BC subtypes. Since the TNBC cells are devoid of receptors, endocrine therapy is an ineffective option for TNBC patients, necessitating canonical chemotherapy strategies to treat TNBC. It is crucial to use alternative and natural agents to support chemotherapy in TNBC. Objectives To clarify the molecular mechanism of the tumorigenic effects of gambogic acid (GA) on TNBC cells with different epithelial character since GA has a wide spectrum of anticancer activity for most cancer types. Methods We determined the cytotoxic dose of GA incubation of TNBC cells (MDA-MB-231 and BT-20 cells) for 24 h. We performed the MTT test and toluidine blue (TB) staining protocol for TNBC cells. We analyzed E-cadherin, N-cadherin, Bax, and neuroserpin mRNAs in both cells by qPCR. We evaluated apoptosis using DAPI staining and assessed the ROS using the 2ʹ,7ʹ-dichlorofluorescin diacetate (DCFH-DA) method. Results We determined the IC50 concentrations of GA in MDA-MB-231 and BT-20 cells to be 315.8 nM and 441.8 nM, respectively. TB staining showed that BT-20 cells survive at excessive cytotoxic doses of GA, while most of the MDA-MB-231 cells were killed. Also, we found that BT-20 cells are more resistant to GA-induced apoptosis and oxidative stress than the MDA-MB-231 cells. qPCR results showed that GA upregulated neuroserpin, an oxidative stress-relieving factor in the BT-20 cells, but not in the MDA-MB-231 cells. Conclusions The elevated level of neuroserpin could be a predictive marker to determine the development of resistance to chemotherapeutic agents.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140834790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cholangiocarcinoma diagnosis and treatment. 胆管癌的诊断和治疗。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-04-30 eCollection Date: 2024-04-01 DOI: 10.2478/abm-2024-0007

引用次数: 0

Cutaneous metastasis in hypopharyngeal carcinoma: a case report. 下咽癌的皮肤转移：病例报告。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-03-20 eCollection Date: 2024-02-01 DOI: 10.2478/abm-2024-0006

Rafia Shahzad, Tooba Anjum, Abu Baker Shahid

{"title":"Cutaneous metastasis in hypopharyngeal carcinoma: a case report.","authors":"Rafia Shahzad, Tooba Anjum, Abu Baker Shahid","doi":"10.2478/abm-2024-0006","DOIUrl":"10.2478/abm-2024-0006","url":null,"abstract":"Background: Occurrence of cutaneous metastasis in hypopharyngeal carcinoma is an extremely rare event reported in the literature, with an incidence of only 0.8%-1.3%. Early diagnosis of cutaneous metastasis would have a positive impact on treatment response and disease prognosis with diagnosis mainly dependent on physical examination and radiological imaging (ultrasonography, computed tomography scan or PET-CT). Palliative care is, however, the mainstay of treatment for cutaneous metastasis.Case presentation: We report a middle-aged female patient, with known case of hypopharyngeal squamous cell carcinoma, who initially showed partial response to chemoradiotherapy but developed cutaneous nodules in the region of the right axilla and bilateral lateral chest wall posterior to the posterior axillary fold. Excision biopsy of one of these nodules showed metastatic squamous cell carcinoma. The patient was again referred to the Oncology Department of INMOL Hospital and her chemotherapy was planned for cutaneous metastasis.Conclusion: Being uncommon, the occurrence of cutaneous lesions in a patient with hypopharyngeal carcinoma should prompt detailed evaluation to rule out metastasis. Early detection will help in improving disease prognosis and median survival.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10953843/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Management of ectopic pregnancy after in vitro fertilization/intracytoplasmic sperm injection and embryo transfer: a case series and mini-review. 体外受精/卵胞浆内单精子注射和胚胎移植后异位妊娠的处理：病例系列和微型综述。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-03-20 eCollection Date: 2024-02-01 DOI: 10.2478/abm-2024-0004

Yang Zhang, Yan Zhai, Danni Qu

{"title":"Management of ectopic pregnancy after in vitro fertilization/intracytoplasmic sperm injection and embryo transfer: a case series and mini-review.","authors":"Yang Zhang, Yan Zhai, Danni Qu","doi":"10.2478/abm-2024-0004","DOIUrl":"10.2478/abm-2024-0004","url":null,"abstract":"Background: Ectopic pregnancy (EP), reflecting a fertilized ovum implanted outside the normal uterine cavity, represents a frequent cause of morbidity and possibly mortality in women of reproductive age.Objective: To summarize the diagnosis and treatment of EP after in vitro fertilization/intracytoplasmic sperm injection and embryo transfer (IVF/ICSI-ET).Methods: The medical records of patients who were diagnosed with EP after embryo transfer from 2017 to 2019, in a tertiary hospital were reviewed.Results: Of the 24 cases analyzed, 21 (87.5%) had fallopian tube involvement, while 2 (8.3%) and 1 (4.2%) had cornual and cervical pregnancies, respectively. Clinical manifestations included vaginal bleeding (58.3%) and lower abdominal pain (16.7%); 9 (42.9%) cases had no symptoms. One cornual pregnancy was misdiagnosed as acute appendicitis and later correctly diagnosed by laparoscopic exploration. There were 2 cases of multiple-site EP and 2 of heterotopic pregnancy, including one with an intrauterine pregnancy with double chorionic and four amniotic sacs and right tubal ampullary pregnancy. Five of the 21 cases with fallopian tube involvement received conservative treatment, while the remaining 16 underwent surgeries, including laparoscopic ipsilateral salpingostomy and ipsilateral salpingectomy.Discussion: Ectopic pregnancy after embryo transfer, mainly involving the fallopian tube, is very complex and is with diverse manifestations. Even with the pregnancy sac observed in the uterus, the pelvic cavity should be scanned thoroughly after embryo transfer.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10953842/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China. 在中国基层医院建立并应用反向点印迹法检测 13 种听力损失基因突变。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-03-20 eCollection Date: 2024-02-01 DOI: 10.2478/abm-2024-0003

Qing-Qing Jiang, Juan-Juan Zhu, Shu-Ling Fan, Ya-Ping Hou, Xie-Ying Hu, Jie Shi, Lei Wu, Ying Luo

{"title":"Establishment and application of a reverse dot blot assay for 13 mutations of hearing-loss genes in primary hospitals in China.","authors":"Qing-Qing Jiang, Juan-Juan Zhu, Shu-Ling Fan, Ya-Ping Hou, Xie-Ying Hu, Jie Shi, Lei Wu, Ying Luo","doi":"10.2478/abm-2024-0003","DOIUrl":"10.2478/abm-2024-0003","url":null,"abstract":"Background: Hearing loss is a common sensorineural dysfunction with a high incidence in China. Although genetic factors are important causes of hearing loss, hearing-related gene detection has not been widely adopted in China.Objective: Establishing a rapid and efficient method to simultaneously detect hotspot hearing loss gene mutations.Methods: A reverse dot blot assay combined with a flow-through hybridization technique was developed for the simultaneous detection of 13 hotspot mutations of 4 hearing loss-related genes including GJB2, GJB3, SLC26A4, and the mitochondrial gene MT-RNR1. This method involved PCR amplification systems and a hybridization platform.Results: The technique can detect 13 hotspot mutations of 4 hearing loss-related genes. And a total of 213 blood samples were used to evaluate the availability of this method.Discussion: Our reverse dot blot assay was a simple, rapid, accurate, and cost-effective method to identify hotspot mutations of 4 hearing loss-related genes in a Chinese population.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10953844/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183639","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-based diets in patients with chronic kidney disease. 慢性肾病患者的植物性饮食。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-03-20 eCollection Date: 2024-02-01 DOI: 10.2478/abm-2024-0002

Wannasit Wathanavasin, Piyawan Kittiskulnam, Kirsten L Johansen

引用次数: 0

Early detection and management of hearing loss to improve the quality of life. 及早发现和管理听力损失，提高生活质量。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-03-20 eCollection Date: 2024-02-01 DOI: 10.2478/abm-2024-0001

引用次数: 0

Reliability of lateral wall-thickness measurement in trochanteric fractures and parameters associated with lateral wall integrity. 转子骨折侧壁厚度测量的可靠性以及与侧壁完整性相关的参数。

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2024-03-20 eCollection Date: 2024-02-01 DOI: 10.2478/abm-2024-0005

Chindarat Ratanakornphan, Thanachart Pikulhom, Somjet Jenvorapoj, Chavarin Amarase, Saran Tantavisut

{"title":"Reliability of lateral wall-thickness measurement in trochanteric fractures and parameters associated with lateral wall integrity.","authors":"Chindarat Ratanakornphan, Thanachart Pikulhom, Somjet Jenvorapoj, Chavarin Amarase, Saran Tantavisut","doi":"10.2478/abm-2024-0005","DOIUrl":"10.2478/abm-2024-0005","url":null,"abstract":"Background: The AO Foundation/Orthopaedic Trauma Association (AO/OTA) introduced a new trochanteric fracture classification in January 2018, concerning the lateral wall integrity. It suggested the intramedullary nail fixation in patients with an incompetent lateral wall fracture.Objective: To determine the reliability of lateral wall-thickness measurement and the fracture parameters associated with lateral wall integrity.Methods: This retrospective study evaluated patients with an intertrochanteric fracture who had had surgery in King Chulalongkorn Memorial Hospital between January 2014 and January 2019. The lateral wall was measured by anteroposterior plain radiography by four raters, two times each. The demographic data and fracture parameters were assessed and compared with respect to lateral wall integrity.Result: In a total of 236 femurs and 232 patients having the 2018 AO/OTA-specified 31A1 and 31A2 intertrochanteric fractures, the lateral wall-thickness measurement showed excellent inter-rater reliability at 0.944 (0.927-0.957) and good-to-excellent intra-rater reliability ranging from 0.835 to 0.972. The parameters associated with lateral wall incompetence as per the multivariate logistic regression analysis were fracture angle (odds ratio [OR] = 0.95), distal greater trochanter involvement (OR = 9.47), and fragments at the intertrochanter area (OR = 4.49) and at the lesser trochanter (OR = 2.6).Conclusion: Some of the parameters related to trochanteric fractures are associated with lateral wall incompetence. Lateral wall-thickness measurement is a reproducible method, which has been suggested for use by the AO/OTA 2018 classification. It is easy to use and can help select the appropriate treatment for intertrochanteric fracture patients.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2024-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10954081/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140183642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lateral flow assay: a promising rapid point-of-care testing tool for infections and non-communicable diseases 侧流检测：一种用于感染和非传染性疾病的前景广阔的快速护理点检测工具

IF 0.6 4区医学

Asian Biomedicine Pub Date : 2023-12-28 DOI: 10.2478/abm-2023-0068

Kumaravel Vealan, Narcisse Joseph, Sharizah Alimat, Anandi S. Karumbati, Karuppiah Thilakavathy

{"title":"Lateral flow assay: a promising rapid point-of-care testing tool for infections and non-communicable diseases","authors":"Kumaravel Vealan, Narcisse Joseph, Sharizah Alimat, Anandi S. Karumbati, Karuppiah Thilakavathy","doi":"10.2478/abm-2023-0068","DOIUrl":"https://doi.org/10.2478/abm-2023-0068","url":null,"abstract":"The point-of-care testing (POCT) approach has established itself as having remarkable importance in diagnosing various infectious and non-communicable diseases (NCDs). The POCT approach has succeeded in meeting the current demand for having diagnostic strategies that can provide fast, sensitive, and highly accurate test results without involving complicated procedures. This has been accomplished by introducing rapid bioanalytical tools or biosensors such as lateral flow assays (LFAs). The production cost of these tools is very low, allowing developing countries with limited resources to utilize them or produce them on their own. Thus, their use has grown in various fields in recent years. More importantly, LFAs have created the possibility for a new era of incorporating nanotechnology in disease diagnosis and have already attained significant commercial success worldwide, making POCT an essential approach not just for now but also for the future. In this review, we have provided an overview of POCT and its evolution into the most promising rapid diagnostic approach. We also elaborate on LFAs with a special focus on nucleic acid LFAs.","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":null,"pages":null},"PeriodicalIF":0.6,"publicationDate":"2023-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139068136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0