Asian Biomedicine最新文献

{"title":"Acute kidney injury in urological conditions.","authors":"Kittisak Weerapolchai, Nuttha Lumlertgul, Nattachai Srisawat, Marlies Ostermann","doi":"10.2478/abm-2026-0002","DOIUrl":"10.2478/abm-2026-0002","url":null,"abstract":"<p><p>Acute kidney injury (AKI) is a sudden deterioration of kidney function, typically diagnosed by a rise in serum creatinine (SCr) and/or a decrease in urine output. In urological conditions, AKI can result from a variety of factors such as an obstruction of the genitourinary tract, urological procedures, trauma, vascular causes, nephrotoxic drugs, or infection. Imaging plays an integral part in the diagnosis of post-renal AKI. Novel kidney biomarkers may have roles in the diagnosis and prognostication of urology-associated AKI. Finally, management consists of the correction of the underlying cause and supportive care. The prognosis of AKI in urological conditions depends on the underlying cause, the timeliness of intervention, and the patient's baseline kidney health.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 1","pages":"3-20"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intrabiliary ultrasound applied to endoscopic extraction of choledochal stones.","authors":"Liang Ye, Huaiyang Cai, Yingwei Wang, Weiqiang Guo","doi":"10.2478/abm-2026-0004","DOIUrl":"10.2478/abm-2026-0004","url":null,"abstract":"<p><strong>Background: </strong>Although ERCP with endoscopic sphincterotomy is effective for choledocholithiasis, small residual stones may be missed by cholangiography, and intraductal ultrasound may improve detection and reduce recurrence.</p><p><strong>Objective: </strong>To observe the value of intraductal ultrasound (IDUS) in endoscopic extraction of choledocholithiasis.</p><p><strong>Methods: </strong>We retrospectively analyzed 148 patients with choledocholithiasis who underwent endoscopic retrograde cholangiopancreatography (ERCP) combined with endoscopic sphincterotomy (EST). IDUS was performed to observe whether there were any residual choledocholithiasis stones in those whose previous choledocholithiasis treatment indicated complete clearance; ERCP combined with EST was repeated to retrieve larger residual stones (≥3 mm). Postoperative follow-up was conducted to monitor the recurrence of choledocholithiasis and its influencing factors.</p><p><strong>Results: </strong>After 148 cases of endoscopic ERCP combined with EST, cholangiography confirmed the complete removal of choledochal stones. Notably, 61 cases were found to have residual stones, and 21 of them had residual stones ≥3 mm; these 21 cases were repeated until the stones were completely removed. After 3-24 months of follow-up, IDUS revealed that the stone recurrence rates were 8.33% (9/108) for 108 cases with complete stone removal and 62.50% (25/40) for 40 cases with residual stones. The difference was statistically significant (<i>P</i> < 0.01). The cumulative recurrence rates during the 24-month postoperative period were also significantly different (88.40% and 14.40%, respectively, <i>P</i> < 0.01). The results of multivariate analysis showed that choledocholithiasis, common bile duct (CBD) diameter, and angle were independent risk factors for stone recurrence, as indicated by IDUS (<i>P</i> < 0.05).</p><p><strong>Conclusions: </strong>IDUS can detect choledocholithiasis, particularly small stones (<3 mm), which are challenging to visualize with cholangiography. IDUS can assist in ERCP combined with EST to reduce the recurrence of postoperative stones.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 1","pages":"35-40"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E-selectin, but not CRP, partially mediates the association between metabolic indices and insulin resistance in older adults: a mediation analysis.","authors":"Laith Ashour, Tariq Albweitel, Juana Abu Rahmeh, Momen Alfawadleh, Afaf El Sharabi, Rahmah Ahmad Shareah, Zaid Alomari, Arar Alhawwari, Mohammad Alkalouti, Noura F Al-Nawaiseh, Malak S Ababneh, Abdelrahman Kaoukji","doi":"10.2478/abm-2026-0007","DOIUrl":"10.2478/abm-2026-0007","url":null,"abstract":"<p><strong>Background: </strong>Evidence on the pathophysiology of insulin resistance (IR), particularly the mediating role of inflammatory markers, remains limited.</p><p><strong>Objectives: </strong>Prior studies suggest associations of C-reactive protein (CRP) and E-selectin with IR, generally examined independently of other risk factors. This study evaluates their potential mediation roles.</p><p><strong>Methods: </strong>Using biomarker data from the Midlife in the United States 3 (MIDUS3) 2017-2022 cohort, we conducted cross-sectional bias-corrected bootstrapping mediation analyses to assess whether CRP and E-selectin mediate associations between established risk factors-body mass index (BMI), waist-hip ratio (WHR), total cholesterol/HDL ratio, age, glycated hemoglobin A (HbA1c), smoking status, dietary habits, physical activity, medication use, and sex-and IR measured by log-transformed homeostatic model assessment of IR (HOMA-IR).</p><p><strong>Results: </strong>The study included 708 participants (57% female; mean age 66.2 ± 9.65 years). CRP did not mediate associations between covariates and HOMA-IR. In contrast, E-selectin showed indirect-only mediation for sex and HOMA-IR (% change in HOMA-IR [%CHIR] in females = 2.4; 95% CI: [0.46, 5.80]). Partial mediation by E-selectin was observed for HbA1c (%CHIR = 9.26; 95% CI: [3.40, 18.66]), BMI (%CHIR = 2.72; 95% CI: [0.97, 5.54]), and total/HDL cholesterol ratio (%CHIR = 1.92; 95% CI: [0.34, 4.49]). Indirect-only mediation was also found for age (%CHIR = -1.11; 95% CI: [-2.58, -0.27]), non-smoking (%CHIR = -1.55; 95% CI: [-4.09, -0.13]), and higher healthy eating index score (%CHIR = -7.87; 95% CI: [-17.48, -2.43]).</p><p><strong>Conclusion: </strong>E-selectin, but not CRP, mediates relationships between metabolic risk factors and IR, highlighting endothelial dysfunction as a key pathway.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 1","pages":"60-69"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135146/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association of clinical characteristics and liver stiffness measurement with liver viscosity measured by shear wave elastography in patient with non-alcoholic fatty liver disease.","authors":"Anchasa Opasatian, Natthaporn Tanpowpong, Pisit Tangkijvanich","doi":"10.2478/abm-2026-0006","DOIUrl":"10.2478/abm-2026-0006","url":null,"abstract":"<p><strong>Background: </strong>Viscosity plane-wave ultrasound (Vi.PLUS) measures tissue viscosity by using shear wave dispersion within tissues. The tissue viscosity is associated with the degree of liver necroinflammation, which is an important mechanism that leads to the development of liver fibrosis (LF).</p><p><strong>Objectives: </strong>This study aimed to examine the association of clinical characteristics and liver stiffness measurement (LSM) by magnetic resonance elastography (MRE) with liver viscosity measurement by shear wave elastography (SWE) in patients with non-alcoholic fatty liver disease.</p><p><strong>Methods: </strong>This monocentric cross-sectional study with retrospective data collection included 164 patients. The medical records of patients aged ≥18 years with non-alcoholic fatty liver disease (NAFLD) and who underwent MRE and Vi.PLUS at King Chulalongkorn Memorial Hospital were reviewed. The viscosity measurements were performed according to guidelines.</p><p><strong>Results: </strong>Of the 164 patients, 11 (6.7%) had invalid or unreliable measurements by elastographic technique and 4 (2.4%) were excluded due to incomplete clinical data. This resulted in 149 subjects being included in the final analysis. The study found good correlation between viscosity measurements by shear wave elastography and LSM by MRE (<i>r</i> = 0.74, <i>P</i> < 0.001). Multivariate regression analysis found that body mass index (<i>P</i> = 0.032) and LSM by MRE (<i>P</i> < 0.001) were independently associated with Vi.PLUS values.</p><p><strong>Conclusion: </strong>In conclusion, viscosity measured by shear wave elastography is a non-invasive method of assessing liver inflammation with good correlation to LF. This may aid the early diagnosis of liver inflammation in patients with NAFLD.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 1","pages":"1-7"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-guided 5% dextrose perineural therapy at the axillary nerve for the treatment of shoulder pain in patients with rotator cuff tendinopathy: a randomized, double-blind, sham-controlled trial.","authors":"Timporn Vitoonpong, Suda Pipatbannakij, Sopatip Rerkmoung, Chernkhuan Stonsaovapak, Natthiya Tantisiriwat","doi":"10.2478/abm-2026-0014","DOIUrl":"10.2478/abm-2026-0014","url":null,"abstract":"<p><strong>Background: </strong>5% dextrose perineural injection therapy (PIT) is a low-cost, accessible, and non-toxic pain relief treatment. No study examined the effects of PIT using a 5% dextrose solution for axillary nerve-related shoulder pain.</p><p><strong>Objectives: </strong>We aimed to investigate the effects of 5% dextrose PIT in the proximal part of the axillary nerve (pAxN) and distal part of the axillary nerve (dAxN) compared to a sham injection, combined with self-exercise in patients with rotator cuff tendinopathy.</p><p><strong>Methods: </strong>This randomized controlled trial included 40 patients with moderate to severe pain from rotator cuff tendinopathy. Participants were randomly assigned to one of 3 groups: 5% dextrose PIT in pAxN, dAxN, or a sham injection. All groups performed self-exercise at home. Pain was measured using the visual analog scale (VAS), and secondary outcomes included the oxford shoulder score (OSS) and range of motion (ROM). Blinding was maintained for participants and assessors.</p><p><strong>Results: </strong>After excluding one participant, 39 individuals (13 per group) were included in the analysis. The dAxN group showed significant pain reduction at 2 and 4-weeks, with estimated mean VAS reductions of -2.46 (-3.87, -1.05) and -1.49 (-2.89, -0.08), respectively, compared to the sham group. However, the pAxN group did not demonstrate a significant difference in pain reduction compared to the sham group. There were no significant differences between groups in other outcomes.</p><p><strong>Conclusions: </strong>Combining 5% dextrose PIT at the dAxN region with self-exercise led to superior short-term pain reduction in patients with rotator cuff tendinopathy. Nonetheless, further large-scale clinical trials are necessary to validate these findings.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 2","pages":"130-141"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268666/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic value of the De Ritis ratio for 1-year major adverse cardiovascular events in elderly patients with acute decompensated heart failure.","authors":"Truong Huy Hoang, Dan Van Buu Do, Nguyen Khoi Dang, Thao Le Phuong Nguyen","doi":"10.2478/abm-2026-0012","DOIUrl":"10.2478/abm-2026-0012","url":null,"abstract":"<p><strong>Background: </strong>The De Ritis ratio, derived from serum aspartate aminotransferase and alanine aminotransferase levels, has shown prognostic value in cardiovascular diseases. Its role in elderly patients hospitalized with acute decompensated heart failure (ADHF) remains uncertain.</p><p><strong>Objectives: </strong>To evaluate the prognostic value of the De Ritis ratio for 1-year major adverse cardiovascular events (MACEs) in elderly patients with ADHF.</p><p><strong>Methods: </strong>A single-center, prospective cohort of 249 patients aged ≥65 years (median age 78, 53.8% female) hospitalized with ADHF was enrolled. Patients were categorized into tertiles by De Ritis ratio: <1.18 (n = 82), 1.18-1.49 (n = 88), and ≥l.49 (n = 79). The primary outcome was 1-year MACEs, including all-cause death, non-fatal myocardial infarction, stroke, and heart failure (HF) rehospitalization. Cox regression was used to identify independent predictors.</p><p><strong>Results: </strong>At 1 year, 68 patients (27.3%) died and 150 (60.2%) experienced MACEs. The event rate increased progressively across tertiles: 31.7%, 64.8%, and 84.8% (<i>P</i> < 0.001). The De Ritis ratio was significantly associated with age, diabetes mellitus, advanced HF symptoms, N-terminal pro-B-type natriuretic peptide, and reduced left ventricular ejection fraction. The optimal cutoff for predicting MACEs was 1.30 (C-statistic: 0.760, 95% confidence interval [CI]: 0.697-0.823, <i>P</i> < 0.001), with 78% sensitivity and 72.7% specificity. A De Ritis ratio >1.30 independently predicted outcomes (hazard ratio: 2.362; 95% CI: 1.536-3.634; <i>P</i> < 0.001).</p><p><strong>Conclusions: </strong>An elevated De Ritis ratio is an independent predictor of 1-year MACEs in elderly patients with ADHF. This simple, accessible biomarker may aid clinical risk stratification.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 2","pages":"114-122"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268670/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arterial cerebral air embolism occurred during root canal treatment: a case report and literature review.","authors":"Beibei Ge, Xinqiang Zhu, Yingxin Ju, Yannan Ma, Yong Song","doi":"10.2478/abm-2026-0015","DOIUrl":"10.2478/abm-2026-0015","url":null,"abstract":"<p><strong>Background: </strong>Root canal treatment is generally safe, but cerebral arterial air embolism (CAAE) is a rare, life-threatening complication.</p><p><strong>Case presentation: </strong>A 69-year-old male with apical periodontitis of teeth #31 and #41 suffered sudden loss of consciousness and coma during endodontic treatment. Cerebral computed tomography (CT) revealed multiple intracranial punctate gas densities with cervical and maxillofacial subcutaneous emphysema. Hyperbaric oxygen therapy (HBOT) led to complete neurological recovery.</p><p><strong>Conclusion: </strong>Clinicians must recognise the risk of arterial air embolism from high-pressure air syringes. Prevention hinges on minimising air pressure and duration, using the lowest effective pressure, and ensuring meticulous haemostasis. Acute neurological deterioration demands immediate imaging and urgent HBOT. Integrating these measures into dental safety standards is essential.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 2","pages":"142-146"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268662/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Best evidence for fluid resuscitation nursing in hypovolemic shock patients in emergency care based on GRADE system.","authors":"Yulian Wang, Jiansheng Lin, Yanhui Lin","doi":"10.2478/abm-2026-0009","DOIUrl":"10.2478/abm-2026-0009","url":null,"abstract":"<p><strong>Background: </strong>Hypovolemic shock is a life-threatening condition frequently encountered in emergency care, resulting from significant blood or fluid loss that impairs oxygen delivery to vital organs. Common causes include trauma, surgery, gastrointestinal bleeding, and ruptured ectopic pregnancy. Prompt fluid resuscitation is critical, with nurses playing a central role in early recognition, timely intervention, and continuous monitoring.</p><p><strong>Objective: </strong>To synthesise the best evidence for fluid resuscitation practices in hypovolemic shock and assess nursing knowledge and practice in emergency settings.</p><p><strong>Methods: </strong>This systematic review was conducted using preferred reporting items for systematic reviews and metaanalyses (PRISMA) guidelines and evaluated through the grading of recommendations assessment, development, and evaluation (GRADE) system. Databases including PubMed, EMBASE, and Cochrane Library were searched for studies published between 2015 and 2024.</p><p><strong>Results: </strong>The isotonic crystalloids are the preferred first-line therapy, with early goal-directed fluid administration and close patient monitoring improving outcomes. Nurse-led assessment and reassessment were key to guiding resuscitation and reducing complications. However, gaps in protocol adherence, documentation, and practice variation were identified. Knowledge deficits among nurses were also evident, highlighting the need for targeted training.</p><p><strong>Conclusions: </strong>This review supports the development of a structured nursing guideline for managing hypovolemic shock, emphasising evidence-based fluid strategies and continuous clinical evaluation. Strengthening nursing competencies through education and standardised protocols can enhance emergency care delivery and patient survival.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 2","pages":"72-84"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268672/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148262940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Favipiravir treatment in Thai children with COVID-19: effectiveness, safety, and modeling drug exposure.","authors":"Sujittra Chaisavaneeyakorn, Nopporn Apiwattanakul, Chonnamet Techasaensiri, Nattakarn Tantawarak, Bandit Thinkhamrop, Supattra Rungmaitree, Siriporn Phongjitsiri, Tavitiya Sudjaritruk, Suvaporn Anugulruengkitt, Pope Kosalaraksa, Thirapa Nivesvivat, Puttichart Khantee, Pornumpa Bunjoungmanee, Panit Takkinsatian, Thiraporn Kanjanaphan, Rudiwilai Samakoses, Tim R Cressey, Henry Pertinez, Andrew Owen, Kulkanya Chokephaibulkit","doi":"10.2478/abm-2026-0011","DOIUrl":"10.2478/abm-2026-0011","url":null,"abstract":"<p><strong>Background: </strong>Despite widespread favipiravir (FPV) use for coronavirus disease 2019 (COVID-19) in Thailand, pediatric clinical evidence remains limited.</p><p><strong>Objectives: </strong>This study evaluated FPV's effectiveness and safety for treating COVID-19 in children and adolescents.</p><p><strong>Methods: </strong>This multicenter, retrospective, observational cohort study included patients ≤18 years old testing COVID-19 positive between July 2021 and December 2022 across 11 hospitals in Thailand. Patients were divided into those with and without chest X-ray-confirmed pneumonia. Patients who received FPV were treated with 35 mg/kg twice daily on day 1 and 15 mg/kg twice daily on days 2-5. Pharmacokinetic (PK) modeling estimated plasma exposure in children using scaled Thai adult data.</p><p><strong>Results: </strong>Of 2,999 patients, 68.2% had no pneumonia. FPV recipients (n = 1,886) were mostly <5 years old, had a fever, infected during the Omicron wave, had lower mean cycle threshold (Ct) values of SARS-CoV-2 gene at diagnosis, and hospitalized compared to non-recipients. FPV treatment significantly shortened fever duration and hospitalization in both pneumonia and non-pneumonia patients. For non-pneumonia patients, being <5 years old, and receiving FPV were associated with complete recovery at discharge. For pneumonia patients, a history of preterm birth, pulmonary disease, and ICU admission reduced the likelihood of complete recovery at discharge. No serious adverse events were reported. PK models predicted that FPV plasma trough concentrations in children ≥10 kg were above efficacy targets.</p><p><strong>Conclusions: </strong>Early FPV treatment shortened fever duration and hospital stays in children and adolescents with COVID-19, promoting clinical recovery in both pneumonia and non-pneumonia cases.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 2","pages":"96-113"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13268665/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148263006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biosynthesis of fosfomycin-loaded CuO nanoparticles: evaluation of antibacterial, antibiofilm properties and molecular docking analysis against biofilm-associated proteins in MDR bacteria.","authors":"Zukhra Abbasi, Fehmida Fasim, Sehrish Abbas, Rabia Shafique, Barkat Ali Khan, Amna Nisar, Sultan M Alshahrani, Bushra Uzair","doi":"10.2478/abm-2026-0003","DOIUrl":"10.2478/abm-2026-0003","url":null,"abstract":"<p><strong>Background: </strong>Infectious diseases caused by antibiotic-resistant bacteria pose a significant challenge in healthcare. The development of new antibiotics, while essential, is often hindered by the complexity, cost, and time involved in the process. An alternative approach gaining traction is the conjugation of existing antibiotics with potent antimicrobial agents to improve their efficacy against resistant pathogens.</p><p><strong>Objective: </strong>This study aimed to develop environmentally sustainable and cost-effective copper oxide nanoparticles (CuO NPs) synthesized using bioactive compounds extracted from <i>Curcuma zedoaria</i>.</p><p><strong>Methods: </strong>These nanoparticles were subsequently conjugated with fosfomycin. Physicochemical characterization was carried out using XRD, scanning electron microscopy (SEM), FTIR, and UV-Visible spectroscopy. Release was studied using Franz diffusion cell. Antibacterial efficacy of the pure and fosfomycin-conjugated copper oxide nanoparticles (Fos-CuO NPs) was evaluated against multidrug-resistant (MDR) strains of <i>Staphylococcus aureus</i>, <i>Escherichia coli</i>, and <i>Pseudomonas aeruginosa</i> using the disk diffusion method. The minimum inhibitory concentration (MIC) and antibiofilm activity were determined using the microbroth dilution method. Additionally, molecular docking analysis was performed to examine the interaction of Fos-CuO NPs with biofilm-associated proteins (LecA, CdrA, PslA, PslD, GacA, CupA, DipA, PelA, PelB) in <i>P. aeruginosa</i>.</p><p><strong>Results: </strong>The physicochemical analysis confirmed successful CuO NPs synthesis and their conjugation with fosfomycin. XRD results confirmed the crystalline structure of the nanoparticles, while SEM revealed some agglomerated, irregular spherical shapes. Fos-CuO NPs exhibited greater antibacterial activity against MDR <i>S. aureus</i> (42 mm), <i>E. coli</i> (45 mm), and <i>P. aeruginosa</i> (39 mm) compared with pure CuO NPs (39 mm, 27 mm, and 41 mm, respectively). The docking results showed that the fosfomycin-conjugated nanoparticle exhibited the highest binding affinity for the biofilm-associated proteins Lec A and Pel A, with docking scores of -4.4 kcal/mol and -4.9 kcal/mol, respectively, compared with blank CuO NPs, supporting their potential application as a novel antimicrobial strategy.</p><p><strong>Conclusion: </strong>This research offers significant insights into the green synthesis of fosfomycin-conjugated nanoparticles for addressing the growing challenge of multidrug-resistant bacterial infections.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"20 1","pages":"21-34"},"PeriodicalIF":1.4,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135145/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147810089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}