Asian Biomedicine最新文献

{"title":"Effects of hepatitis B vaccination on hepatitis B surface antigen in neonates and its change in vivo.","authors":"Shuqi Wang,&nbsp;Yuting Bai,&nbsp;Fangyuan Yuan,&nbsp;Ting Wang,&nbsp;Wenyi Luo,&nbsp;Can Luo,&nbsp;Qiang Wang,&nbsp;Dongsheng Wang","doi":"10.2478/abm-2022-0029","DOIUrl":"https://doi.org/10.2478/abm-2022-0029","url":null,"abstract":"<p><strong>Background: </strong>Vaccination is effective to prevent hepatitis B virus (HBV) infection. However, there is still a risk of infection after vaccination. In clinical work, we found that newborns were positive for HBV surface antigen (HBsAg) after vaccination.</p><p><strong>Objectives: </strong>To determine the effect of hepatitis B vaccination on the detection of HBsAg trend in newborns.</p><p><strong>Methods: </strong>We collected data at birth, history of vaccination for hepatitis B, quantitative HBsAg results, and other information about newborns born in our hospital from July 2017 to July 2020. Serum samples from healthy neonates were randomly selected to be supplemented with recombinant hepatitis B vaccine on a concentration gradient, and HBsAg was measured quantitatively.</p><p><strong>Results: </strong>Data from 1417 neonates were included in the study; 306 (21.6%) were HBsAg positive within 8 d after vaccination, with levels ranging from 0.104 IU/mL to 0.339 IU/mL. The proportion of neonates with HBsAg-positive serum was significantly correlated with the level of hepatitis B surface antibodies (anti-HBs) in the serum of their mothers (<i>P</i> < 0.01). Experiments in vitro showed that the proportion of neonates with HBsAg-positive serum was correlated with the dose of the hepatitis B vaccine, and when the concentration of the hepatitis B vaccine reached 5 ng/mL and 10 ng/mL, the serum HBsAg levels showed a significant negative correlation with the original concentration of serum anti-HBs.</p><p><strong>Conclusions: </strong>Hepatitis B vaccination can affect the level of HBsAg detected in neonatal serum, and the effect could be mitigated by delaying the measurement. Moreover, maternal anti-HBs offset the effects of neonatal vaccination on HBsAg serum levels.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"265-272"},"PeriodicalIF":0.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy-related genes affect the survival of multiple myeloma patients depending on chromosomal abnormality.","authors":"Gizem Ayna Duran,&nbsp;Yasemin Benderli Cihan","doi":"10.2478/abm-2022-0028","DOIUrl":"https://doi.org/10.2478/abm-2022-0028","url":null,"abstract":"<p><strong>Background: </strong>Targeting autophagy at gene level may be promising in multiple myeloma (MM) treatment depending on chromosomal abnormality (ABN) status.</p><p><strong>Objectives: </strong>We aimed to investigate the role of ABN on survival of MM patients and to identify prognosis related autophagy-related genes (ARGs) for patients with or without ABN.</p><p><strong>Methods: </strong>Gene intensity values of 222 ARG for 548 MM patients were obtained from the Affymetrix Human Genome U133 Plus 2.0 Array (GPL570) platform containing 54,675 probes (GSE24080). A dataset containing data from 1576 MM patients with 1q21 amplification (GSE4204, GSE4452, GSE4581, and GSE2658) was used for validation. Survival analysis of the patients was analyzed using univariate and multivariate Cox regression method with the help of R3.53 programming language and Kaplan-Meier graphics were created. The Gene Ontology enRIchmentanaLysis and visuaLizAtion (GOrilla) tool was used to define the related biological processes and pathways.</p><p><strong>Results: </strong>The overall survival (OS) and event-free survival (EFS) in all MM patients were strongly influenced by ABN. In the group of patients with ABN, 41 ARGs were found to be important in prognosis, whereas in the group of patients without ABN, 13 ARGs were found to be important in prognosis. <i>CDKN1A</i>, <i>FKBP1B</i>, <i>FOXO3</i>, and <i>NCKAP1</i> ARGs were commonly significant in both groups and found to be survival triggering.</p><p><strong>Conclusions: </strong>The classification of MM patients according to the absence or presence of ABN is important in the determination of survival status. Detection of survival related ARGs in patients with chromosomal anomalies may be a new therapeutic target in treatment.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"249-264"},"PeriodicalIF":0.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A One Health approach to antimicrobial resistance.","authors":"","doi":"10.2478/abm-2022-0019","DOIUrl":"10.2478/abm-2022-0019","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"151-152"},"PeriodicalIF":0.4,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10316305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sialic acid: an attractive biomarker with promising biomedical applications.","authors":"Aida Doostkam, Leila Malekmakan, Alireza Hosseinpour, Sahar Janfeshan, Jamshid Roozbeh, Fatemeh Masjedi","doi":"10.2478/abm-2022-0020","DOIUrl":"10.2478/abm-2022-0020","url":null,"abstract":"<p><p>This broad, narrative review highlights the roles of sialic acids as acidic sugars found on cellular membranes. The role of sialic acids in cellular communication and development has been well established. Recently, attention has turned to the fundamental role of sialic acids in many diseases, including viral infections, cardiovascular diseases, neurological disorders, diabetic nephropathy, and malignancies. Sialic acid may be a target for developing new drugs to treat various cancers and inflammatory processes. We recommend the routine measurement of serum sialic acid as a sensitive inflammatory marker in various diseases.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"153-167"},"PeriodicalIF":0.4,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long noncoding and micro-RNA expression in a model of articular chondrocyte degeneration induced by stromal cell-derived factor-1.","authors":"Guoliang Wang, Lu He, Yaoyu Xiang, Di Jia, Yanlin Li","doi":"10.2478/abm-2022-0021","DOIUrl":"10.2478/abm-2022-0021","url":null,"abstract":"<p><strong>Background: </strong>Gene regulatory network analysis has found that long noncoding ribonucleic acids (lncRNAs) are strongly associated with the pathogenesis of osteoarthritis.</p><p><strong>Objectives: </strong>To determine the differential expression of lncRNAs and microRNAs (miRNAs) in normal chondrocytes and those from a model of articular chondrocyte degeneration.</p><p><strong>Methods: </strong>Chondrocytes were cultured from cartilage obtained from patients diagnosed with osteoarthritis of the knee. Stromal cell-derived factor-1 (SDF-1) was used to induce their degeneration. Total RNA was extracted, analyzed, amplified, labeled, and hybridized on a chip to determine expression. The set of enriched differentially expressed miRNAs was analyzed by gene ontology and the Kyoto Encyclopedia of Genes and Genomes to describe the functional properties of the key biological processes and pathways. We conducted a bioinformatics analysis using Cytoscape to elucidate the interactions between miRNAs and proteins.</p><p><strong>Results: </strong>We found that the expression of 186 lncRNAs was significantly different in the model of chondrocyte degeneration, in which 88 lncRNAs were upregulated, and 98 were downregulated. Expression of 684 miRNAs was significantly different. Analysis of the protein-protein interaction (PPI) network indicated that the genes for CXCL10, ISG15, MYC, MX1, OASL, IFIT1, RSAD2, MX2, IFI44L, and BST2 are the top 10 core genes, identifying the most important functional modules to elucidate the differential expression of miRNAs.</p><p><strong>Conclusions: </strong>These data may provide new insights into the molecular mechanisms of chondrocyte degeneration in osteoarthritis, and the identification of lncRNAs and miRNAs may provide potential targets for the differential diagnosis and therapy of osteoarthritis.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"169-179"},"PeriodicalIF":0.4,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoter methylation analysis of <i>DKK2</i> may be a potential biomarker for early detection of cervical cancer.","authors":"Xian Zhang,&nbsp;Aihua Li,&nbsp;Jie Wu,&nbsp;Yu Wu,&nbsp;Xiaoping Ma,&nbsp;Yanjun Liu,&nbsp;Qingfa Chen,&nbsp;Yan Zhang","doi":"10.2478/abm-2022-0022","DOIUrl":"https://doi.org/10.2478/abm-2022-0022","url":null,"abstract":"<p><strong>Background: </strong>Dickkopf 2 (<i>DKK2</i>) plays an important role in multiple cancers. Its potential value in the clinical diagnosis of cervical cancer has remained unclear.</p><p><strong>Objectives: </strong>To investigate the expression and promoter methylation levels of <i>DKK2</i> in cervical cancer and their clinicopathological associations.</p><p><strong>Methods: </strong>We used the Gene Expression Omnibus, Oncomine, Cancer Genome Atlas, and University of ALabama at Birmingham CANcer data analysis databases, reverse transcription-PCR, and methylation-specific PCR analysis to predict and examine the expression of DKK2 mRNA and <i>DKK2</i> methylation levels in cell lines and cervical cancer tissues from 79 patients with cervical cancer and 63 with cervical precancerous lesions including 25 with low-grade squamous intraepithelial lesions (LSIL) and 38 patients with high-grade squamous intraepithelial lesions (HSIL).</p><p><strong>Results: </strong>DKK2 mRNA expression was downregulated in all cancer cell lines and cervical cancer tissues, whereas hypermethylation of <i>DKK2</i> was higher in cervical cancer tissue samples. <i>DKK2</i> methylation in cervical cancer was significantly higher than that in HSIL (χ² = 8.346, <i>P</i> = 0.004), whereas <i>DKK2</i> methylation in HSIL was significantly higher than that in normal cervical samples (χ² = 7.934, <i>P</i> = 0.005) and in LSIL samples (χ² = 4.375, <i>P</i> = 0.037). <i>DKK2</i> silencing caused by its promoter hypermethylation was confirmed by treatment with the methyltransferase inhibitor 5-Aza-dC in cell lines. Patients with lymph node metastasis exhibited increased promoter methylation frequency (χ² = 5.239, <i>P</i> = 0.022) and low DKK2 mRNA expression (χ² = 3.958, <i>P</i> = 0.047) compared with patients with no lymph node metastasis. Patients with high-risk human papillomavirus infection exhibited increased promoter methylation frequency (χ² = 6.279, <i>P</i> = 0.015).</p><p><strong>Conclusions: </strong><i>DKK2</i> epigenetic changes of DKK2 may play a key role in the development of cervical cancer, suggesting that <i>DKK2</i> hypermethylation could be used as a triage test for screening, early diagnosis, or risk prediction of cervical cancer.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"181-189"},"PeriodicalIF":0.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of <i>Staphylococcus aureus</i> from southern and northeastern India.","authors":"Archana Loganathan,&nbsp;Ramesh Nachimuthu","doi":"10.2478/abm-2022-0023","DOIUrl":"https://doi.org/10.2478/abm-2022-0023","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> is a pathogen endemic in India and sometimes deadly for patients in intensive care units.</p><p><strong>Objectives: </strong>To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of <i>S. aureus</i> from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.</p><p><strong>Methods: </strong>We collected <i>S. aureus</i> isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as <i>mecA</i>, <i>mecC</i>, <i>blaZ</i>, <i>vanA</i>, <i>vanB</i>, and <i>vanC</i> were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.</p><p><strong>Results: </strong>A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant <i>S. aureus</i> in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% <i>mecA</i> in Tamil Nadu and 49% <i>mecA</i> in Mizoram. Vancomycin-intermediate resistance <i>S. aureus</i> (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant <i>S. aureus</i> (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.</p><p><strong>Conclusions: </strong>The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"191-199"},"PeriodicalIF":0.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An 85-amino-acid polypeptide from <i>Myrmeleon bore</i> larvae (antlions) homologous to heat shock factor binding protein 1 with antiproliferative activity against MG-63 osteosarcoma cells in vitro.","authors":"Rui Ding,&nbsp;Ming He,&nbsp;Huoying Huang,&nbsp;Jing Chen,&nbsp;Mingxing Huang,&nbsp;Yonghui Su","doi":"10.2478/abm-2022-0024","DOIUrl":"https://doi.org/10.2478/abm-2022-0024","url":null,"abstract":"<p><strong>Background: </strong>Venomous arthropods have substances in their venom with antiproliferative potential for neoplastic cells.</p><p><strong>Objectives: </strong>To identify a polypeptide from <i>Myrmeleon bore</i> (antlion) with antiproliferative activity against neoplastic cells, and to elucidate the molecular mechanism of the activity.</p><p><strong>Methods: </strong>We used gel filtration and ion exchange chromatography to purify a polypeptide with antiproliferative activity against MG-63 human osteosarcoma cells from a proteinaceous extract of antlion. The polypeptide was sequenced and the stability of its antiproliferative activity was tested under a range of conditions in vitro. An 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine the antiproliferative activity of the polypeptide against the MG-63 osteosarcoma cells and MC3T3-E1 mouse calvarial osteoblasts, which were used as a non-neoplastic control. We used western blotting to compare the levels of expression of heat shock transcription factor 1 (HSF1), heat shock protein 90 (HSP90), cyclin-dependent kinase 4 (CDK4), and protein kinase B alpha (ATK1) in MG-63 osteosarcoma cells and their mouse homologs in MC3T3-E1 osteoblasts after their treatment with the antlion antiproliferative polypeptide (ALAPP).</p><p><strong>Results: </strong>The 85-amino-acid ALAPP has a 56% sequence identity with the human heat shock factor binding protein 1 (HSBP1). The antiproliferative activity of the polypeptide is relatively insensitive to temperature, pH, and metal ions. ALAPP has a strong concentration-dependent antiproliferative activity against MG-63 osteosarcoma cells compared with its effect on MC3T3-E1 osteoblasts. ALAPP significantly upregulates the expression of HSF1 in MC3T3-EL osteoblasts, but not in MG-63 osteosarcoma. ALAPP significantly downregulated the expression of HSP90, CDK4, and AKT1 expression in MG-63 osteosarcoma, but not in the osteoblasts.</p><p><strong>Conclusions: </strong>ALAPP has significant antiproliferative activity against MG-63 osteosarcoma cells, but not nonneoplastic MC3T3-E1 osteoblasts. We speculate that non-neoplastic cells may evade the antiproliferative effect of ALAPP by upregulating HSF1 to maintain their HSP90, CDK4, and AKT1 expression at a relatively constant level.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"201-211"},"PeriodicalIF":0.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321181/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10316302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dichotomous role of autophagy in cancer.","authors":"Amin Arif, Muhammad Babar Khawar, Rabia Mehmood, Muddasir Hassan Abbasi, Nadeem Sheikh","doi":"10.2478/abm-2022-0014","DOIUrl":"10.2478/abm-2022-0014","url":null,"abstract":"<p><p>Autophagy is an evolutionary conserved catabolic process that plays physiological and pathological roles in a cell. Its effect on cellular metabolism, the proteome, and the number and quality of organelles, diversely holds the potential to alter cellular functions. It acts paradoxically in cancer as a tumor inhibitor as well as a tumor promoter. In the early stage of tumorigenesis, it prevents tumor initiation by the so-called \"quality control mechanism\" and suppresses cancer progression. For late-staged tumors that are exposed to stress, it acts as a vibrant process of degradation and recycling that promotes cancer by facilitating metastasis. Despite this dichotomy, the crucial role of autophagy is evident in cancer, and associated with mammalian targets of rapamycin (mTOR), p53, and Ras-derived major cancer networks. Irrespective of the controversy regarding autophagic manipulation, promotion and suppression of autophagy act as potential therapeutic targets in cancer treatment and may provide various anticancer therapies.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 3","pages":"111-120"},"PeriodicalIF":0.4,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9962161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthy collaborations are needed for mangrove land use and mosquito control.","authors":"","doi":"10.2478/abm-2022-0013","DOIUrl":"10.2478/abm-2022-0013","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 3","pages":"109-110"},"PeriodicalIF":0.4,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9962153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}