Asian Biomedicine最新文献

{"title":"Burden of congenital and hereditary anomalies in the war-affected territory at Pakistan-Afghanistan border.","authors":"Muhammad Naeem,&nbsp;Bashir Ahmad,&nbsp;Sajid Malik","doi":"10.2478/abm-2022-2033","DOIUrl":"https://doi.org/10.2478/abm-2022-2033","url":null,"abstract":"<p><strong>Background: </strong>Pashtun populations of Pakistan are the victim of long-lasting military combats, rendering 1.9 million inhabitants internally displaced. Studies highlighting congenital and hereditary anomalies in these populations are deficient.</p><p><strong>Objectives: </strong>To elucidate the spectrum anomalies in the north-western war-affected territories of Pakistan.</p><p><strong>Methods: </strong>A cross-sectional study was carried out from 2017 to 2019 and individuals or families with anomalies were ascertained through convenience and cluster random sampling. Phenotypic and pedigree data and information on bio-demographic variables were collected. Descriptive statistics were applied.</p><p><strong>Results: </strong>A total of 361 independent individuals or families with anomalies were recruited. The anomalies were grouped into 8 major and 72 minor entities. Among major categories, neurological disorders had the highest representation (n = 100; proportion: 0.277; 95% CI: 0.231-0.323), followed by sensorineural defects (n = 70; prop.: 0.194), limb defects (n = 60; prop.: 0.166), visual impairments (n = 55; prop.: 0.152), and musculoskeletal defects (n = 37; prop.: 0.102). Among the neurological disorders, intellectual disability had the highest occurrence (58%), whereas talipes and limb amputations were the most prominent in limb defects (22% and 20%, respectively). The anomalies had sporadic and isolated presentations most often (76% each), while parental consanguinity was observed in 34% of index cases.</p><p><strong>Conclusions: </strong>The high incidence of neurological, sensorineural, and limb defects, the preponderance of sporadic cases, and low level of parental consanguinity may indicate a potentially high contribution of nongenetic factors in the etiology of anomalies. The majority of anomalies are the cause of severe disability.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 6","pages":"299-309"},"PeriodicalIF":0.6,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10392144/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10316319","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence and management of eosinophilia based on periodic health examinations in primary care clinics.","authors":"Thareerat Ananchaisarp,&nbsp;Panya Chamroonkiadtikun,&nbsp;Jakrawadee Julamanee,&nbsp;Kewalee Perdvong,&nbsp;Thitawan Chimpalee,&nbsp;Nutnicha Rattanavirakul,&nbsp;Nattawat Leelarujijaroen,&nbsp;Tiprada Hathaipitak,&nbsp;Thanarat Tantinam","doi":"10.2478/abm-2022-0030","DOIUrl":"https://doi.org/10.2478/abm-2022-0030","url":null,"abstract":"<p><strong>Background: </strong>Eosinophilia is a common, hematologic abnormality detected in periodic health checkups with diverse etiologies. There are a few clinical practice guidelines for the management of eosinophilia.</p><p><strong>Objectives: </strong>To determine the prevalence of eosinophilia among patients undergoing periodic health examinations, evaluate its management and outcomes, and identify its associated factors.</p><p><strong>Methods: </strong>We conducted a retrospective study that included patients with eosinophilia diagnosed during the 2018 periodic health examinations at Songklanagarind Hospital.</p><p><strong>Results: </strong>The prevalence rate of eosinophilia was 9.6% (988/10,299), and most patients (52.6%) were male with a median age of 53.0 (42.0-61.0) years. Only 174 patients (17.6%) were diagnosed and further examined to identify the cause of eosinophilia; including an examination of medical history (18.4%), physical examination (93.1%), laboratory analysis (9.2%), and consultation with internists (14.9%). Empirical anthelmintic therapy was administered in 130 patients (74.7%), and 49.2% achieved resolution. The possible causes of eosinophilia were identified in 20.7% (204/988), the most common cause being atopic disease (51.5%). Patients with moderate-to-severe eosinophilia were significantly more likely to be diagnosed, undergo further laboratory tests, and proceed with consultations with internists (adjusted OR [95% CI] = 3.52 [1.97-6.32], 17.13 [5.74-51.11], and 6.38 [1.95-20.93], respectively).</p><p><strong>Conclusions: </strong>Eosinophilia is commonly identified in periodic health examinations, and most primary physicians lack knowledge regarding the diagnostic work-up required to determine the cause of eosinophilia. Empirical anthelmintic therapy showed satisfactory efficacy for the management of eosinophilia in areas where parasite infection is endemic.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"273-282"},"PeriodicalIF":0.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321192/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-centered education in dyslipidemia management: a systematic review.","authors":"Farhana Fakhira Ismail,&nbsp;Adyani Md Redzuan,&nbsp;Chong Wei Wen","doi":"10.2478/abm-2022-0026","DOIUrl":"https://doi.org/10.2478/abm-2022-0026","url":null,"abstract":"<p><strong>Background: </strong>Dyslipidemia management is crucial to reduce mortality and morbidity from cardiovascular diseases (CVDs). Patients must be educated and empowered to enable them to manage their own diseases. Various methods of patient education, such as patient-centered education (PCE) or non-PCE (such as didactic education or any traditional form of education), have been implemented.</p><p><strong>Objective: </strong>To review and determine the effectiveness of PCE for dyslipidemia management compared with usual care. The primary outcome chosen was cholesterol level. Other measures, such as psychosocial or cognitive, behavioral, and other relevant outcomes, were also extracted. Additionally, underlying theories and other contributing factors that may have led to the success of the intervention were also reviewed and discussed.</p><p><strong>Methods: </strong>We conducted searches in PubMed, Cumulative Index to Nursing and Allied Health Literature (CINAHL), Scopus, and Google Scholar from inception until April 2021. All studies involving randomized controlled trials were included. Study quality was assessed using the Critical Appraisal Skills Program (CASP) checklist specifically for randomized controlled trials.</p><p><strong>Results: </strong>The search identified 8,847 records. Of these, 20 studies were eligible for inclusion. Interventions using a PCE approach were largely successful. Contributing factors extracted from the included studies were underlying theories, instant reward system, dietary education, collaborative care, duration of intervention with systematic follow-ups, social support, adherence assessment method, and usage of e-health.</p><p><strong>Conclusions: </strong>PCE is successful in achieving the desired outcomes in dyslipidemia management. Future studies may incorporate the elements of PCE to improve the management of dyslipidemia in hospital or community settings where appropriate.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"214-236"},"PeriodicalIF":0.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of hepatitis B vaccination on hepatitis B surface antigen in neonates and its change in vivo.","authors":"Shuqi Wang,&nbsp;Yuting Bai,&nbsp;Fangyuan Yuan,&nbsp;Ting Wang,&nbsp;Wenyi Luo,&nbsp;Can Luo,&nbsp;Qiang Wang,&nbsp;Dongsheng Wang","doi":"10.2478/abm-2022-0029","DOIUrl":"https://doi.org/10.2478/abm-2022-0029","url":null,"abstract":"<p><strong>Background: </strong>Vaccination is effective to prevent hepatitis B virus (HBV) infection. However, there is still a risk of infection after vaccination. In clinical work, we found that newborns were positive for HBV surface antigen (HBsAg) after vaccination.</p><p><strong>Objectives: </strong>To determine the effect of hepatitis B vaccination on the detection of HBsAg trend in newborns.</p><p><strong>Methods: </strong>We collected data at birth, history of vaccination for hepatitis B, quantitative HBsAg results, and other information about newborns born in our hospital from July 2017 to July 2020. Serum samples from healthy neonates were randomly selected to be supplemented with recombinant hepatitis B vaccine on a concentration gradient, and HBsAg was measured quantitatively.</p><p><strong>Results: </strong>Data from 1417 neonates were included in the study; 306 (21.6%) were HBsAg positive within 8 d after vaccination, with levels ranging from 0.104 IU/mL to 0.339 IU/mL. The proportion of neonates with HBsAg-positive serum was significantly correlated with the level of hepatitis B surface antibodies (anti-HBs) in the serum of their mothers (<i>P</i> < 0.01). Experiments in vitro showed that the proportion of neonates with HBsAg-positive serum was correlated with the dose of the hepatitis B vaccine, and when the concentration of the hepatitis B vaccine reached 5 ng/mL and 10 ng/mL, the serum HBsAg levels showed a significant negative correlation with the original concentration of serum anti-HBs.</p><p><strong>Conclusions: </strong>Hepatitis B vaccination can affect the level of HBsAg detected in neonatal serum, and the effect could be mitigated by delaying the measurement. Moreover, maternal anti-HBs offset the effects of neonatal vaccination on HBsAg serum levels.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"265-272"},"PeriodicalIF":0.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321203/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy-related genes affect the survival of multiple myeloma patients depending on chromosomal abnormality.","authors":"Gizem Ayna Duran,&nbsp;Yasemin Benderli Cihan","doi":"10.2478/abm-2022-0028","DOIUrl":"https://doi.org/10.2478/abm-2022-0028","url":null,"abstract":"<p><strong>Background: </strong>Targeting autophagy at gene level may be promising in multiple myeloma (MM) treatment depending on chromosomal abnormality (ABN) status.</p><p><strong>Objectives: </strong>We aimed to investigate the role of ABN on survival of MM patients and to identify prognosis related autophagy-related genes (ARGs) for patients with or without ABN.</p><p><strong>Methods: </strong>Gene intensity values of 222 ARG for 548 MM patients were obtained from the Affymetrix Human Genome U133 Plus 2.0 Array (GPL570) platform containing 54,675 probes (GSE24080). A dataset containing data from 1576 MM patients with 1q21 amplification (GSE4204, GSE4452, GSE4581, and GSE2658) was used for validation. Survival analysis of the patients was analyzed using univariate and multivariate Cox regression method with the help of R3.53 programming language and Kaplan-Meier graphics were created. The Gene Ontology enRIchmentanaLysis and visuaLizAtion (GOrilla) tool was used to define the related biological processes and pathways.</p><p><strong>Results: </strong>The overall survival (OS) and event-free survival (EFS) in all MM patients were strongly influenced by ABN. In the group of patients with ABN, 41 ARGs were found to be important in prognosis, whereas in the group of patients without ABN, 13 ARGs were found to be important in prognosis. <i>CDKN1A</i>, <i>FKBP1B</i>, <i>FOXO3</i>, and <i>NCKAP1</i> ARGs were commonly significant in both groups and found to be survival triggering.</p><p><strong>Conclusions: </strong>The classification of MM patients according to the absence or presence of ABN is important in the determination of survival status. Detection of survival related ARGs in patients with chromosomal anomalies may be a new therapeutic target in treatment.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"249-264"},"PeriodicalIF":0.6,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321186/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A One Health approach to antimicrobial resistance.","authors":"","doi":"10.2478/abm-2022-0019","DOIUrl":"10.2478/abm-2022-0019","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"151-152"},"PeriodicalIF":0.4,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321163/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10316305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sialic acid: an attractive biomarker with promising biomedical applications.","authors":"Aida Doostkam, Leila Malekmakan, Alireza Hosseinpour, Sahar Janfeshan, Jamshid Roozbeh, Fatemeh Masjedi","doi":"10.2478/abm-2022-0020","DOIUrl":"10.2478/abm-2022-0020","url":null,"abstract":"<p><p>This broad, narrative review highlights the roles of sialic acids as acidic sugars found on cellular membranes. The role of sialic acids in cellular communication and development has been well established. Recently, attention has turned to the fundamental role of sialic acids in many diseases, including viral infections, cardiovascular diseases, neurological disorders, diabetic nephropathy, and malignancies. Sialic acid may be a target for developing new drugs to treat various cancers and inflammatory processes. We recommend the routine measurement of serum sialic acid as a sensitive inflammatory marker in various diseases.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"153-167"},"PeriodicalIF":0.4,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321195/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long noncoding and micro-RNA expression in a model of articular chondrocyte degeneration induced by stromal cell-derived factor-1.","authors":"Guoliang Wang, Lu He, Yaoyu Xiang, Di Jia, Yanlin Li","doi":"10.2478/abm-2022-0021","DOIUrl":"10.2478/abm-2022-0021","url":null,"abstract":"<p><strong>Background: </strong>Gene regulatory network analysis has found that long noncoding ribonucleic acids (lncRNAs) are strongly associated with the pathogenesis of osteoarthritis.</p><p><strong>Objectives: </strong>To determine the differential expression of lncRNAs and microRNAs (miRNAs) in normal chondrocytes and those from a model of articular chondrocyte degeneration.</p><p><strong>Methods: </strong>Chondrocytes were cultured from cartilage obtained from patients diagnosed with osteoarthritis of the knee. Stromal cell-derived factor-1 (SDF-1) was used to induce their degeneration. Total RNA was extracted, analyzed, amplified, labeled, and hybridized on a chip to determine expression. The set of enriched differentially expressed miRNAs was analyzed by gene ontology and the Kyoto Encyclopedia of Genes and Genomes to describe the functional properties of the key biological processes and pathways. We conducted a bioinformatics analysis using Cytoscape to elucidate the interactions between miRNAs and proteins.</p><p><strong>Results: </strong>We found that the expression of 186 lncRNAs was significantly different in the model of chondrocyte degeneration, in which 88 lncRNAs were upregulated, and 98 were downregulated. Expression of 684 miRNAs was significantly different. Analysis of the protein-protein interaction (PPI) network indicated that the genes for CXCL10, ISG15, MYC, MX1, OASL, IFIT1, RSAD2, MX2, IFI44L, and BST2 are the top 10 core genes, identifying the most important functional modules to elucidate the differential expression of miRNAs.</p><p><strong>Conclusions: </strong>These data may provide new insights into the molecular mechanisms of chondrocyte degeneration in osteoarthritis, and the identification of lncRNAs and miRNAs may provide potential targets for the differential diagnosis and therapy of osteoarthritis.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"169-179"},"PeriodicalIF":0.4,"publicationDate":"2022-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promoter methylation analysis of <i>DKK2</i> may be a potential biomarker for early detection of cervical cancer.","authors":"Xian Zhang,&nbsp;Aihua Li,&nbsp;Jie Wu,&nbsp;Yu Wu,&nbsp;Xiaoping Ma,&nbsp;Yanjun Liu,&nbsp;Qingfa Chen,&nbsp;Yan Zhang","doi":"10.2478/abm-2022-0022","DOIUrl":"https://doi.org/10.2478/abm-2022-0022","url":null,"abstract":"<p><strong>Background: </strong>Dickkopf 2 (<i>DKK2</i>) plays an important role in multiple cancers. Its potential value in the clinical diagnosis of cervical cancer has remained unclear.</p><p><strong>Objectives: </strong>To investigate the expression and promoter methylation levels of <i>DKK2</i> in cervical cancer and their clinicopathological associations.</p><p><strong>Methods: </strong>We used the Gene Expression Omnibus, Oncomine, Cancer Genome Atlas, and University of ALabama at Birmingham CANcer data analysis databases, reverse transcription-PCR, and methylation-specific PCR analysis to predict and examine the expression of DKK2 mRNA and <i>DKK2</i> methylation levels in cell lines and cervical cancer tissues from 79 patients with cervical cancer and 63 with cervical precancerous lesions including 25 with low-grade squamous intraepithelial lesions (LSIL) and 38 patients with high-grade squamous intraepithelial lesions (HSIL).</p><p><strong>Results: </strong>DKK2 mRNA expression was downregulated in all cancer cell lines and cervical cancer tissues, whereas hypermethylation of <i>DKK2</i> was higher in cervical cancer tissue samples. <i>DKK2</i> methylation in cervical cancer was significantly higher than that in HSIL (χ² = 8.346, <i>P</i> = 0.004), whereas <i>DKK2</i> methylation in HSIL was significantly higher than that in normal cervical samples (χ² = 7.934, <i>P</i> = 0.005) and in LSIL samples (χ² = 4.375, <i>P</i> = 0.037). <i>DKK2</i> silencing caused by its promoter hypermethylation was confirmed by treatment with the methyltransferase inhibitor 5-Aza-dC in cell lines. Patients with lymph node metastasis exhibited increased promoter methylation frequency (χ² = 5.239, <i>P</i> = 0.022) and low DKK2 mRNA expression (χ² = 3.958, <i>P</i> = 0.047) compared with patients with no lymph node metastasis. Patients with high-risk human papillomavirus infection exhibited increased promoter methylation frequency (χ² = 6.279, <i>P</i> = 0.015).</p><p><strong>Conclusions: </strong><i>DKK2</i> epigenetic changes of DKK2 may play a key role in the development of cervical cancer, suggesting that <i>DKK2</i> hypermethylation could be used as a triage test for screening, early diagnosis, or risk prediction of cervical cancer.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"181-189"},"PeriodicalIF":0.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiotic resistance, biofilm forming ability, and clonal profiling of clinical isolates of <i>Staphylococcus aureus</i> from southern and northeastern India.","authors":"Archana Loganathan,&nbsp;Ramesh Nachimuthu","doi":"10.2478/abm-2022-0023","DOIUrl":"https://doi.org/10.2478/abm-2022-0023","url":null,"abstract":"<p><strong>Background: </strong><i>Staphylococcus aureus</i> is a pathogen endemic in India and sometimes deadly for patients in intensive care units.</p><p><strong>Objectives: </strong>To determine the antibiotic-resistance pattern, biofilm forming ability, and clonal type of <i>S. aureus</i> from isolates collected in Tamil Nadu (south) and the Mizoram (northeast) regions of India.</p><p><strong>Methods: </strong>We collected <i>S. aureus</i> isolates from diagnostic laboratories in Tamil Nadu and Mizoram. An antibiotic susceptibility test was performed according to Clinical Laboratory and Standards Institute methods. Antibiotic-resistant determinants such as <i>mecA</i>, <i>mecC</i>, <i>blaZ</i>, <i>vanA</i>, <i>vanB</i>, and <i>vanC</i> were confirmed by polymerase chain reaction (PCR). All isolates were further studied for biofilm forming ability. Enterobacterial repetitive intergenic consensus (ERIC)-PCR was used for clonal analysis.</p><p><strong>Results: </strong>A study of 206 clinical isolates showed 52.9% prevalence of methicillin-resistant <i>S. aureus</i> in Tamil Nadu and 49.4% in Mizoram. Minimum inhibitory concentration tests showed a high prevalence of 67% oxacillin resistance in isolates from Tamil Nadu and 49% in isolates from Mizoram. PCR showed 53% <i>mecA</i> in Tamil Nadu and 49% <i>mecA</i> in Mizoram. Vancomycin-intermediate resistance <i>S. aureus</i> (VISA) prevalence was lower in isolates from Tamil Nadu (4%) and Mizoram (5%). All methicillin-resistant <i>S. aureus</i> (MRSA) isolates formed biofilms. Clonal analysis revealed a genetic relatedness between the isolates.</p><p><strong>Conclusions: </strong>The prevalence of MRSA is high in the regions studied, with most of the clinical isolates being multidrug resistant. Adopting appropriate community-based preventive measures and establishing antimicrobial stewardship is highly recommended to minimize the dissemination in antibiotic resistance.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 4","pages":"191-199"},"PeriodicalIF":0.6,"publicationDate":"2022-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9959653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}