Asian Biomedicine最新文献

{"title":"In silico prediction of deleterious non-synonymous SNPs in <i>STAT3</i>.","authors":"Athira Ajith,&nbsp;Usha Subbiah","doi":"10.2478/abm-2023-0059","DOIUrl":"https://doi.org/10.2478/abm-2023-0059","url":null,"abstract":"<p><strong>Background: </strong><i>STAT3</i>, a pleiotropic transcription factor, plays a critical role in the pathogenesis of autoimmunity, cancer, and many aspects of the immune system, as well as having a link with inflammatory bowel disease. Changes caused by non-synonymous single nucleotide polymorphisms (nsSNPs) have the potential to damage the protein's structure and function.</p><p><strong>Objective: </strong>We identified disease susceptible single nucleotide polymorphisms (SNPs) in <i>STAT3</i> and predicted structural changes associated with mutants that disrupt normal protein-protein interactions using different computational algorithms.</p><p><strong>Methods: </strong>Several <i>in silico</i> tools, such as SIFT, PolyPhen v2, PROVEAN, PhD-SNP, and SNPs&GO, were used to determine nsSNPs of the <i>STAT3</i>. Further, the potentially deleterious SNPs were evaluated using I-Mutant, ConSurf, and other computational tools like DynaMut for structural prediction.</p><p><strong>Result: </strong>417 nsSNPs of <i>STAT3</i> were identified, 6 of which are considered deleterious by <i>in silico</i> SNP prediction algorithms. Amino acid changes in V507F, R335W, E415K, K591M, F561Y, and Q32K were identified as the most deleterious nsSNPs based on the conservation profile, structural conformation, relative solvent accessibility, secondary structure prediction, and protein-protein interaction tools.</p><p><strong>Conclusion: </strong>The in silico prediction analysis could be beneficial as a diagnostic tool for both genetic counseling and mutation confirmation. The 6 deleterious nsSNPs of <i>STAT3</i> may serve as potential targets for different proteomic studies, large population-based studies, diagnoses, and therapeutic interventions.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 4","pages":"185-199"},"PeriodicalIF":0.6,"publicationDate":"2023-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49673791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of transversus abdominis plane block application on postoperative analgesia quality and patient satisfaction after varicocele surgery: a randomized clinical trial.","authors":"Ömer Faruk Boran,&nbsp;Aykut Urfalioglu,&nbsp;Mahmut Arslan,&nbsp;Fatih Mehmet Yazar,&nbsp;Bora Bilal,&nbsp;Yavuz Orak,&nbsp;Bülent Katı,&nbsp;Ayşe Azak Bozan","doi":"10.2478/abm-2023-0053","DOIUrl":"https://doi.org/10.2478/abm-2023-0053","url":null,"abstract":"<p><strong>Background: </strong>Postoperative pain management is an important aspect of anesthesia care and multimodal analgesic techniques are generally recommended.</p><p><strong>Objective: </strong>To compare the effect of spinal anesthesia + transversus abdominis plane (TAP) block application on postoperative analgesia quality and patient satisfaction with spinal anesthesia + intrathecal morphine (ITM) application.</p><p><strong>Methods: </strong>A total of 70 patients were randomly separated into 2 groups as spinal anesthesia + TAP block (TAP block group, n = 34) and spinal anesthesia + ITM group (ITM group, n = 36). The groups were compared in respect of age, body mass index values, and visual analog scale (VAS) values at 0 h, 2 h, 6 h, 12 h, and 18 h, and patient satisfaction was scored by Quality Improvement in Postoperative Pain Management at 24 h.</p><p><strong>Results: </strong>The mean age of the patients was 32.52 ± 6.50 years in the TAP block group and 30.11 ± 5.62 years in the ITM group, with no statistically significant difference determined. There was no statistically significant difference in terms of VAS values at 0 h, 2 h, 6 h, 12 h, and 18 h. When the factors affecting postoperative patient satisfaction were evaluated, feeling fatigue after the surgery (r = -0.811, <i>P</i> = 0.001) and postoperative complications such as nausea, vomiting, and itching (r = -0.831, <i>P</i> = 0.001) were found to have a negative effect on patient satisfaction.</p><p><strong>Conclusion: </strong>Due to low complication rates, TAP block is an effective application for postoperative analgesia management in varicocele operations that increases patient satisfaction postoperatively.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"136-143"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561680/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-quality contact tracing, case investigation, and isolation of suspected and confirmed cases: a key strategy to control the spread of infection.","authors":"","doi":"10.2478/abm-2023-0049","DOIUrl":"10.2478/abm-2023-0049","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"93-94"},"PeriodicalIF":0.4,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561676/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-30c-2-3p suppresses the proliferation of human renal cell carcinoma cells by targeting TOP2A.","authors":"Xiaoyong Huang,&nbsp;Yuna Jia,&nbsp;Haiyan Shi,&nbsp;Haiyan Fan,&nbsp;Lingbo Sun,&nbsp;Huahua Zhang,&nbsp;Yanfeng Wang,&nbsp;Jie Chen,&nbsp;Jiaqi Han,&nbsp;Mingming Wang,&nbsp;Juan Du,&nbsp;Jing Zhang","doi":"10.2478/abm-2023-0052","DOIUrl":"https://doi.org/10.2478/abm-2023-0052","url":null,"abstract":"<p><strong>Background: </strong>The ambiguity of renal cell carcinoma (RCC) symptoms hinders early diagnosis, thereby contributing to high mortality rates. By attaching to the 3'-untranslated region (UTR) of the target gene, microRNAs (miRNAs) exert significant control over the expression of genes.</p><p><strong>Objectives: </strong>To investigate the influence of miR-30c-2-3p and DNA topoisomerase II alpha (TOP2A) on RCC growth and the mechanisms underlying the regulation of its expression.</p><p><strong>Methods: </strong>The expression of miRNA-30c-2-3p and <i>TOP2A</i> in RCC cells was examined using quantitative real-time polymerase chain reaction (qRT-PCR). MiR-30c-2-3p mimics, its inhibitors, and controls, as well as TOP2A short hairpin RNA (shRNA) and controls, were used to transfect the human RCC cell lines 786-O, Caki-1, and ACHN. Additionally, the roles of miRNA-30c-2-3p and TOP2A in the growth of RCC were evaluated using the cell counting kit (CCK)-8 test, colony formation assay, apoptosis analysis, and Western blotting. Meanwhile, binding of miRNA-30c-2-3p and TOP2A was verified using dual-luciferase reporter assays and Western blotting.</p><p><strong>Results: </strong>miR-30c-2-p is underexpressed in RCC cells. Overexpression of miR-30c-2-p promotes apoptosis and inhibits proliferation of ACHN, Caki-1, and 786-O cells. miR-30c-2-3p targets TOP2A, which is elevated in RCC tissues and cells, whereas TOP2A silencing inhibits the proliferation ability of RCC cells. The miRNA-30c-2-3p inhibitor compromises TOP2A shRNA-induced apoptosis of RCC. RCC cells cotransfected with miRNA-30c-2-3p inhibitors and TOP2A shRNAs have a higher proliferation rate than those transfected with only TOP2A shRNAs.</p><p><strong>Conclusions: </strong>Collectively, our results verify that miRNA-30c-2-3p has a tumor suppressor property. miRNA-30c-2-3p inhibits the proliferation of RCC through regulation of TOP2A. The data provide a viable therapeutic target for RCC.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"124-135"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561683/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between inflammatory biomarkers and macular pigment optical density in hypertensive patients: a cross-sectional study.","authors":"Chunchuree Kongmeesuk Kaneko,&nbsp;Katsunori Kaneko,&nbsp;Vitoon Jularattanaporn,&nbsp;Thamthiwat Nararatwanchai","doi":"10.2478/abm-2023-0054","DOIUrl":"10.2478/abm-2023-0054","url":null,"abstract":"<p><strong>Background: </strong>Inflammation may be associated with macular pigment optical density (MPOD) degradation.</p><p><strong>Objectives: </strong>The relationship between inflammation and MPOD is evaluated using inflammatory biomarkers, including high sensitivity C-reactive protein (hsCRP), lipid level and ratio, waist circumference (WC), and body mass index (BMI).</p><p><strong>Method: </strong>In this cross-sectional design, 62 hypertensive patients were recruited between January 6 and January 8, 2022, at a primary care unit. The MPOD was measured using the Macular pigment screener II. Blood tests for hsCRP, lipid profile, WC measurement, BMI calculation, and completing a questionnaire were conducted, and statistical analysis was done by using Microsoft Excel 2019 and Stata version 16.1. Spearman's rank correlation test was used to evaluate correlations. Multivariate analysis for adjusting confounders was done by logistic regression.</p><p><strong>Result: </strong>There was a significant negative correlation between hsCRP >3 and MPOD (<i>r</i> = -0.26, <i>P</i> = 0.04).</p><p><strong>Conclusion: </strong>Inflammation was linked to MPOD. Anti-inflammatory agents may be beneficial in preventing MPOD degradation.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"144-149"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561677/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Infection rate among close contacts of patients with coronavirus disease in Japan: a descriptive study and literature review.","authors":"Osamu Yamamura,&nbsp;Hidenori Onishi,&nbsp;Ippei Sakamaki,&nbsp;Ryousuke Fujita,&nbsp;Hirofumi Miyashita,&nbsp;Hiromichi Iwasaki","doi":"10.2478/abm-2023-0051","DOIUrl":"10.2478/abm-2023-0051","url":null,"abstract":"<p><strong>Background: </strong>In Japan, on April 20, 2020, the definition of a close contact regarding coronavirus disease (COVID-19) was changed from a long-term contact time to a specified contact time of 15 min and from a contact distance of 2 m to 1 m.</p><p><strong>Objectives: </strong>We aimed to determine the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection rate among close contacts of patients with COVID-19 and determine the impact of the infection on transmission among close contacts.</p><p><strong>Methods: </strong>The numbers of SARS-CoV-2 tests, SARS-CoV-2-positive cases, and close contacts of patients with COVID-19 were assessed between March 2020 and February 2021 in Fukui Prefecture, Japan. The study period was subdivided into 3 periods. The second and third period contained data with the changed definition of close contact.</p><p><strong>Results: </strong>Overall, 32,238 SARS-CoV-2 tests were performed. There were 545 patients with COVID-19 and 1487 close contacts, of whom 267 tested positive. The highest infection rate occurred in period 3. Distance, protective measures, and contact time with COVID-19 patients influenced the increased infection rate. The infection rate showed a rising trend from 11.1% in period 1 to 19.2% and 20.0% in periods 2 and 3, respectively (Cochran-Armitage test; <i>P</i> < 0.004). Multivariate analysis revealed that female sex was an independent risk factor for infection of close contacts (odds ratio: 2.23; 95% confidence interval: 1.700-2.930).</p><p><strong>Conclusions: </strong>Female sex is a risk factor for transmission by close contacts. The rate of infection among close contacts may be associated with contact time, contact distance, and protective measures.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"115-123"},"PeriodicalIF":0.6,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561678/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacogenomics of response to statin treatment and susceptibility to statin-induced adverse drug reactions in Asians: a scoping review.","authors":"Hui-Yin Yow, Sharina Hamzah, Nusaibah Abdul Rahim, Vijayaprakash Suppiah","doi":"10.2478/abm-2023-0050","DOIUrl":"10.2478/abm-2023-0050","url":null,"abstract":"<p><strong>Background: </strong>Statins are the most widely used lipid-lowering agents for patients with hyperlipidemia. However, interindividual variations in efficacy and risk of adverse drug reactions to statin treatment have been widely reported. Ethnicity is well known to be one of the contributing factors to this variation, particularly among Asians.</p><p><strong>Objectives: </strong>To identify genetic variants associated with statin treatment responses among Asian populations with a focus on four commonly prescribed statins: atorvastatin, rosuvastatin, simvastatin, and pravastatin.</p><p><strong>Methods: </strong>A literature search was conducted in Medline and Embase databases. Studies published from 2008 to 2021 were included. The title and abstract of each article were screened by two reviewers and verified by another two reviewers. Data charted include information on authors, year of study, study population, statin studied, gene studied, study findings, and data of significant statistical value.</p><p><strong>Results: </strong>A total of 35 articles were included from the 1,939 original studies related to treatment efficacy and 5 articles out of the 284 original studies related to adverse effects. Genetic variants in transmembrane transporters, cytochrome P450 isoenzymes, and apolipoproteins are the most extensively studied among Asian populations, with a main focus on ethnic Chinese. However, Asia consists of genetically different populations, and the results of this review indicated that there is a paucity of studies on other ethnic groups within Asia.</p><p><strong>Conclusions: </strong>Considering the ethnicity of patients could provide a potential value to personalized medicine in statin therapy.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"17 3","pages":"95-114"},"PeriodicalIF":0.4,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10561688/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41189516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and environmental risk of congenital anomaly.","authors":"","doi":"10.2478/abm-2022-0031","DOIUrl":"10.2478/abm-2022-0031","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 6","pages":"283-284"},"PeriodicalIF":0.4,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10392141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10013540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ameliorative effect of different mesoporous bioactive glass materials in experimental tibial defects in rats.","authors":"Ozlem Ozmen, Fatma Tomul, Yusuf Sinan Sirin","doi":"10.2478/abm-2022-0027","DOIUrl":"10.2478/abm-2022-0027","url":null,"abstract":"<p><strong>Background: </strong>Enhancing the bone healing procedure would resultantly improve the post-recovery life quality, as well as the speed with which the patient returns to their former life quality. Porous structures can provide a large surface area and abundant channels to facilitate mass transfer.</p><p><strong>Objective: </strong>To evaluate the application of mesoporous materials in the bone healing of surgically created defects on the tibiae of male adult Wistar rats.</p><p><strong>Methods: </strong>The defect areas were evaluated after implantation of 4 types of bioactive glass histopathologically and immunohistochemically. Fifty adult rats were divided into 5 groups including a control group without material. The used products were mesoporous bioactive glass (MBG), Cu-MBG, Zn-MBG, and Cu-Zn-MBG. Unicortical bone defects with a 3 mm diameter were performed in both tibiae of the animals and filled with 4 types of glass particles. The rats were then euthanized at 15 d and 30 d. Tibial samples were collected and the tissues forwarded for histological processing, and examined using light microscopy. Additionally, bone healing was evaluated by assessing the levels of bone morphogenetic protein BMP2, collagen 1, osteocalcin (OST), and vascular endothelial growth factor (VEGF) using immunohistochemical methods.</p><p><strong>Results: </strong>Within the 15th day, all groups presented connective tissue septa; at the 30th day, the new bone formation was more intense in the Cu-Zn-MBG group. Additionally, BMP2, collagen 1, OST, and VEGF immune expression were more prominent in the Cu-Zn-MBG group.</p><p><strong>Conclusions: </strong>The study results indicated that MBG may be used for the repairing of bone defects. Cu-Zn-MBG may be the best choice for this purpose.</p>","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"237-248"},"PeriodicalIF":0.4,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321190/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patient-centered high-value integrated care.","authors":"","doi":"10.2478/abm-2022-0025","DOIUrl":"10.2478/abm-2022-0025","url":null,"abstract":"","PeriodicalId":8501,"journal":{"name":"Asian Biomedicine","volume":"16 5","pages":"212-213"},"PeriodicalIF":0.4,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10321183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9950547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}