Archives of Medical Research最新文献

{"title":"Clinical characteristics and outcomes of infective endocarditis in patients with cancer: A multicenter case-control study","authors":"Gustavo Mendez-Hirata ,&nbsp;Frida Rivera-Buendía ,&nbsp;Eduardo Viveros-Rentería ,&nbsp;Eric Ochoa-Hein ,&nbsp;Patricia Cornejo-Juárez ,&nbsp;Manlio Márquez ,&nbsp;José Ramón Hidalgo-Dura ,&nbsp;Yoana Leyva-López ,&nbsp;Diana Vilar-Compte","doi":"10.1016/j.arcmed.2024.103123","DOIUrl":"10.1016/j.arcmed.2024.103123","url":null,"abstract":"<div><h3>Background</h3><div>Due to increased exposure to health hazards, patients with cancer are at a higher risk of developing infective endocarditis (IE), which in turn maximizes their risk of poor outcomes. This study aimed to analyze IE events in patients with cancer and matched controls.</div></div><div><h3>Methods</h3><div>We conducted a retrospective case-control study in four third-level centers in Mexico City between 2006 and 2022. Patients with IE were identified; cases (patients with cancer) were matched in a 1:2 ratio with controls (patients without cancer). Baseline characteristics, factors associated with IE, and outcomes were assessed in both groups.</div></div><div><h3>Results</h3><div>A total of 108 patients were included (36 cases and 72 controls). After logistic regression analysis, the risk factors independently associated with IE in cases were obesity (adjusted odds ratio [aOR] 9.03, 95 % CI 1.5–51.8), surgery within six months before IE (aOR 6.23, 95 % CI 1.8–21.5), and invasive procedures within six months prior to IE (aOR 3.89, 95 % CI 1.15–13.1). Healthcare-associated IE was more common in these cases, as were systemic embolic episodes. Subjects with <em>S. aureus</em> IE were more prone to experience systemic embolization. There were no differences in mortality between the groups.</div></div><div><h3>Conclusion</h3><div>In this study, risk factors associated with the healthcare environment were more frequent in patients with IE and cancer. <em>S. aureus</em> was a common culprit and was associated with systemic embolization.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103123"},"PeriodicalIF":4.7,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 in Venezuela: Costs and challenges of management severe cases at home in a crisis setting","authors":"David A. Forero-Peña ,&nbsp;Fhabián S. Carrión-Nessi ,&nbsp;Óscar D. Omaña-Ávila ,&nbsp;Daniela L. Mendoza-Millán ,&nbsp;Augusto Moncada-Ortega ,&nbsp;María E. Landaeta","doi":"10.1016/j.arcmed.2024.103126","DOIUrl":"10.1016/j.arcmed.2024.103126","url":null,"abstract":"<div><div>The humanitarian crisis in Venezuela has become an unprecedented regional emergency. Over the past decade, Venezuela's political and economic collapse has eroded the country's healthcare infrastructure and threatened the public health of the population. Shortages of medicines and health supplies, disruptions in basic services, and the emigration of health workers during this humanitarian crisis have affected access to care throughout the country, as well as the capacity of emergency services and outbreak response. During the early waves of the epidemic, COVID-19 collapsed the Venezuelan health system, leaving sentinel centers without inpatient beds and ICU space. Home-based care alternatives quickly emerged, not only for mild to moderate but also for severe and critical COVID-19 cases. Here we discuss the impact of the pandemic on the Venezuelan health system, the emergence of home-based care alternatives for severe and critical COVID-19 cases, as well as their costs and challenges, and finally, their advantages and disadvantages.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103126"},"PeriodicalIF":4.7,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated serum and urinary secreted protein acidic and rich in cysteine levels are novel biomarkers of kidney fibrosis severity","authors":"Yang-yang Niu ,&nbsp;Ying Yu ,&nbsp;Wen-qian Zhou ,&nbsp;Xiao-qin Zhang ,&nbsp;Sai-ya Zhu ,&nbsp;Ying-ying Zhang ,&nbsp;Xin Li ,&nbsp;Hong-ping Shan ,&nbsp;Jian-ying Niu ,&nbsp;Tian-jun Guan ,&nbsp;Chen Yu","doi":"10.1016/j.arcmed.2024.103125","DOIUrl":"10.1016/j.arcmed.2024.103125","url":null,"abstract":"<div><h3>Background</h3><div>Interstitial fibrosis is the primary determinant of the progression of chronic kidney disease (CKD), and noninvasive identification of interstitial fibrosis is a major challenge. We aimed to explore the diagnostic value of secreted protein acidic and rich in cysteine (SPARC) in serum and urine in kidney fibrosis.</div></div><div><h3>Methods</h3><div>Single-cell transcriptome analysis was used to measure SPARC expression in healthy reference kidneys and those of patients with CKD. A total of 674 patients with CKD who underwent renal biopsy served as the training cohort (<em>n</em> = 322) and the validation cohort (<em>n</em> = 352). Serum and urinary SPARC levels were measured at the time of kidney biopsy. In vivo and in vitro models of kidney fibrosis were also used to confirm the role of SPARC.</div></div><div><h3>Results</h3><div>Increased SPARC expression was detected in kidney fibrosis tissues. Higher serum SPARC levels were associated with increased severity of kidney fibrosis. Moreover, the area under the receiver operating characteristic curve (AUC-ROC) (AUC 0.86) was greater for the serum SPARC level than for the urinary SPARC level and estimated glomerular filtration rate (eGFR). The combination of the serum and urinary SPARC levels and eGFR increased the AUC-ROC for predicting kidney fibrosis from 0.86 to 0.90. The diagnostic performance of serum or urinary SPARC levels was consistent in the validation cohort. In vivo and in vitro models of kidney fibrosis also confirmed the upregulation of SPARC expression.</div></div><div><h3>Conclusions</h3><div>Serum and urinary SPARC levels may be potential biomarkers for kidney fibrosis and may be useful for noninvasive diagnosis.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103125"},"PeriodicalIF":4.7,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722410","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Laparoscopic robotic assisted surgery in reproductive medicine","authors":"Julio Ricardo Loret de Mola","doi":"10.1016/j.arcmed.2024.103137","DOIUrl":"10.1016/j.arcmed.2024.103137","url":null,"abstract":"<div><div>Laparoscopic Robot-assisted surgery is one of the most promising and rapidly developing surgical advancements of the twenty-first century with a potential to make significant contributions to reproductive medicine and preservation of fertility. Presently, laparoscopic robotic assisted surgery is used for various benign and malignant gynecological procedures, including fertility enhancing procedures. Laparoscopic Robot-assisted surgery is superior to traditional open procedures with regards to post-surgical hospital stay and blood loss; however, the difference is comparable to laparoscopic surgery. Regarding operative times, the results have been inconsistent due to variations in surgeons’ experience. The primary drawbacks of robotic systems are their high installation and maintenance costs and historical lack of tactile feedback; however, this has been overcome by the most recent evolution of robotic systems. Along with the major advances in cancer therapy, the number of female cancer survivors of reproductive age has dramatically increased. Consequently, fertility preservation and fertility enhancement have gained more emphasis in reproductive surgery in the last decade. A broad range of surgical procedures such as tubal reanastomosis, myomectomy, treatment of deep infiltrating endometriosis, ovarian transposition, radical trachelectomy, and ovarian transplantation has been introduced to restore or preserve fertility using Laparoscopic Robot-assisted surgery. In this article, we aim to present the current applications, advantages, and disadvantages of Laparoscopic Robot-assisted surgical technology in the field of reproductive surgery, including the more recent advances of Artificial Intelligence (AI) in the field.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103137"},"PeriodicalIF":4.7,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm human biobanking: An overview","authors":"Alfredo Góngora ,&nbsp;William V. Holt ,&nbsp;Jaime Gosálvez","doi":"10.1016/j.arcmed.2024.103130","DOIUrl":"10.1016/j.arcmed.2024.103130","url":null,"abstract":"<div><div>The purpose of this article is to analyze in detail the advantages and disadvantages of sperm cryopreservation, focusing on the cellular and molecular changes that occur during these processes. The main issue is the cellular damage caused by ice crystal formation and osmotic imbalance, along with other secondary effects such as sperm motility and viability, as well as the acrosome reaction or oxidative stress. Another important aspect is the examination of how chromatin structure and DNA integrity affect sperm. Biochemical changes affecting enzyme activity and protein stability have also been analyzed. Finally, the article highlights emerging technologies aimed at reducing the damage caused by sperm cryopreservation, as well as the potential benefits of biobanks as an essential resource for addressing male infertility.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103130"},"PeriodicalIF":4.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nutritional status and incidence of hepatocellular carcinoma in patients with compensated liver cirrhosis","authors":"Marlene López-Sánchez ,&nbsp;Aleida Bautista-Santos ,&nbsp;María del Pilar Milke-García ,&nbsp;Aldo Allende-López ,&nbsp;Rosalba Moreno-Alcántar ,&nbsp;Segundo Morán","doi":"10.1016/j.arcmed.2024.103127","DOIUrl":"10.1016/j.arcmed.2024.103127","url":null,"abstract":"<div><h3>Background</h3><div>Malnutrition in patients with liver cirrhosis (LC) and/or hepatocellular carcinoma (HCC) has been associated with adverse outcomes. However, there is little information on the incidence of HCC during the compensated phase of LC in relation to the nutritional status.</div></div><div><h3>Aim</h3><div>To evaluate the association between the incidence of HCC in compensated LC and their nutritional status.</div></div><div><h3>Methods</h3><div>Patients with compensated liver cirrhosis with no previous history of ascites, hepatic encephalopathy, or variceal bleeding attending the Gastroenterology outpatient service at Centro Medico Nacional Siglo XXI were included in a prospective cohort. Clinical and nutritional parameters were collected, including the Royal Free Hospital Subjective Global Assessment (RFH-SGA) as an indicator of protein-calorie malnutrition and the triceps skinfold thickness, which classified patients as having <em>normal</em> subcutaneous adipose tissue (SAT), <em>above average</em> SAT, and <em>below average</em> SAT. Follow-up was censored at the time of HCC diagnosis or LC decompensation.</div></div><div><h3>Results</h3><div>About 31/187 (16.0 %) and 22/187 (11.8 %) patients were categorized as having above- or below-average SAT at baseline, respectively. 10/187 patients (5.3 %) developed HCC during the compensated phase of LC at a median of 22 months (IQR: 10.0–36.75). A higher risk of HCC was observed in subjects below average SAT (HR: 4.064, CI 95 %: 1.012–16.317, <em>p</em> = 0.048). After adjusting the Cox models for age and α-fetoprotein at baseline, the statistical significance of the association between SAT and HCC was not modified.</div></div><div><h3>Conclusion</h3><div>These results suggest that decreased SAT may precede the diagnosis of HCC in compensated LC.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103127"},"PeriodicalIF":4.7,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deficiency of miR-155 in leukemic B-cells results in cell cycle arrest and deregulation of MIR155HG/TP53INP1/CDKN1A/CCND1 network","authors":"Elena Golovina ,&nbsp;Juraj Kokavec ,&nbsp;Dmitry Kazantsev ,&nbsp;Oxana Yurikova ,&nbsp;Martin Bajecny ,&nbsp;Filipp Georgijevic Savvulidi ,&nbsp;Radim Simersky ,&nbsp;Rene Lenobel ,&nbsp;Jorg Tost ,&nbsp;Vit Herynek ,&nbsp;Ludek Sefc ,&nbsp;Marek Sebela ,&nbsp;Pavel Klener ,&nbsp;Zuzana Zemanova ,&nbsp;Tomas Stopka ,&nbsp;Karina Savvulidi Vargova","doi":"10.1016/j.arcmed.2024.103124","DOIUrl":"10.1016/j.arcmed.2024.103124","url":null,"abstract":"<div><h3>Background</h3><div>Cell cycle progression and leukemia development are tightly regulated processes in which even a small imbalance in the expression of cell cycle regulatory molecules and microRNAs (miRNAs) can lead to an increased risk of cancer/leukemia development. Here, we focus on the study of a ubiquitous, multifunctional, and oncogenic miRNA-hsa-miR-155–5p (miR-155, <em>MIR155HG</em>), which is overexpressed in malignancies including chronic lymphocytic leukemia (CLL). Nonetheless, the precise mechanism of how miR-155 regulates the cell cycle in leukemic cells remains the subject of extensive research.</div></div><div><h3>Methods</h3><div>We edited the CLL cell line MEC-1 by CRISPR/Cas9 to introduce a short deletion within the <em>MIR155HG</em> gene. To describe changes at the transcriptome and miRNome level in miR-155-deficient cells, we performed mRNA-seq/miRNA-seq and validated changes by qRT-PCR. Flow cytometry was used to measure cell cycle kinetics. A WST-1 assay, hemocytometer, and Annexin V/PI staining assessed cell viability and proliferation.</div></div><div><h3>Results</h3><div>The limited but phenotypically robust miR-155 modification impaired cell proliferation, cell cycle, and cell ploidy. This was accompanied by overexpression of the negative cell cycle regulator <em>p21/CDKN1A</em> and <em>Cyclin D1</em> (<em>CCND1</em>). We confirmed the overexpression of canonical miR-155 targets such as <em>PU.1, FOS, SHIP-1, TP53INP1</em> and revealed new potential targets (<em>FCRL5, ISG15,</em> and <em>MX1</em>).</div></div><div><h3>Conclusions</h3><div>We demonstrate that miR-155 deficiency impairs cell proliferation, cell cycle, transcriptome, and miRNome via deregulation of the <em>MIR155HG/TP53INP1/CDKN1A/CCND1</em> axis. Our CLL model is valuable for further studies to manipulate miRNA levels to revert highly aggressive leukemic cells to nearly benign or non-leukemic types.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103124"},"PeriodicalIF":4.7,"publicationDate":"2024-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142698344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A ten-year experience with the diagnosis of von Willebrand disease in Mexico based on a cost-effective strategy","authors":"Jesus Hernandez-Juarez ,&nbsp;Victor Manuel Dominguez-Reyes ,&nbsp;Jaime Garcia-Chavez ,&nbsp;Manuel Moreno-Hernandez ,&nbsp;Paola Itzel Carmona-Olvera ,&nbsp;Jose Antonio Alvarado-Moreno ,&nbsp;Guillermo Espejo-Godinez ,&nbsp;Julieta Espinoza-Islas ,&nbsp;Karim Majluf-Cruz ,&nbsp;Rodrigo Arreola-Diaz ,&nbsp;Patricia Cruz-Puente ,&nbsp;Guadalupe Ortiz-Torres ,&nbsp;Berenice Sanchez-Jara ,&nbsp;Cecilia Rodriguez-Castillejos ,&nbsp;Rosa Elena Sosa-Camas ,&nbsp;Patricia Gomez-Rosas ,&nbsp;Gregorio Campos-Cabrera ,&nbsp;Abraham Majluf-Cruz","doi":"10.1016/j.arcmed.2024.103113","DOIUrl":"10.1016/j.arcmed.2024.103113","url":null,"abstract":"<div><h3>Background</h3><div>Von Willebrand disease (VWD), is the most common inherited bleeding disorder worldwide, but its diagnosis is complicated, expensive, and poorly evaluated in several countries.</div></div><div><h3>Objective</h3><div>To report our long-term experience with the diagnosis of VWD based on a cost-effective strategy.</div></div><div><h3>Methods</h3><div>We studied 802 Mexican patients, men and women, children, and adults, with clinical suspicion of VWD. The following tests were performed: blood count, bleeding time, prothrombin time, activated partial thromboplastin time, fibrinogen concentration, VWF antigen, ristocetin cofactor activity, collagen binding assay, ristocetin-induced platelet aggregation, FVIII activity, and VWF multimers analysis.</div></div><div><h3>Results</h3><div>VWD was diagnosed in 639 patients; 582 had type 1 VWD (91.1%). Type 2 VWD was found in 52 patients (8.1%). Type 2A was present in 25 cases (48.1%), while types 2B and 2 M accounted for 21 (40.4) and six (11.5%) cases, respectively. Type 3 VWD was present in five patients (0.8%). The mean age of patients with VWD was 25.3 years (range: 2–71) for males and 22.1 (range: 1–54) for females. The diagnosis was inconclusive in 40 cases (5.0%) and was discarded in 123 (15.3%). Blood group O was the most common among patients with VWD.</div></div><div><h3>Conclusion</h3><div>Using a low-cost diagnostic strategy, we confirmed that VWD is as common in Mexico as in other countries. Review of the patient's history is mandatory when VWD is suspected, although laboratory confirmation may be difficult and expensive. The consequences of a lack of accurate and timely diagnosis affect the promptness and quality of treatment.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 2","pages":"Article 103113"},"PeriodicalIF":4.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Risk Phenotypes for Type 2 Diabetes Differ with Ancestry in US Adults with Diabetes and Overweight/Obesity","authors":"Lauren A. Fowler ,&nbsp;José R Fernández ,&nbsp;Patrick M. O'Neil ,&nbsp;Vibhu Parcha ,&nbsp;Pankaj Arora ,&nbsp;Naman S. Shetty ,&nbsp;Michelle I. Cardel ,&nbsp;Gary D. Foster ,&nbsp;Barbara A Gower","doi":"10.1016/j.arcmed.2024.103128","DOIUrl":"10.1016/j.arcmed.2024.103128","url":null,"abstract":"<div><h3>Background</h3><div>Type 2 diabetes (T2D) risk is higher among non-Hispanic black (NHB) and Hispanic individuals, for reasons that are unclear.</div></div><div><h3>Aims</h3><div>With this cross-sectional study, we tested the hypothesis that racial disparities in T2D prevalence can be partially traced to heterogeneity in etiology, as indicated by genetic subtypes that reflect distinct T2D phenotypes.</div></div><div><h3>Methods</h3><div>Using a diverse sample of 361 US adults with T2D (69.5% women; 34.1% NHB; 13.9% Hispanic), we derived genetic risk scores (GRS) representing five distinct T2D pathophysiological pathways from 94 loci: β-cell, proinsulin, obesity, lipodystrophy, and liver/lipid. Genetic predisposition for insulin resistance (IR) was also assessed using a 52-SNP IR risk score.</div></div><div><h3>Results</h3><div>The β-cell and proinsulin scores (as median [IQR]) were higher among NHB participants relative to NHW and Hispanics (β-cell GRS [NHB, 0.842(0.784–0.887) vs. NHW, 0.762(0.702–0.835) and Hispanic, 0.772(0.717–0.848)]); proinsulin GRS (NHB, 1.006[0.973–1.070] vs. NHW, 0.969[0.853–1.044] and Hispanic, 0.976[0.901–1.048]), whereas the liver/lipid and 52-SNP IR scores were higher in both NHB and Hispanic participants versus NHW (liver/lipid GRS [NHB, 1.09(0.78–1.18) and Hispanic, 0.895(0.736–1.227) vs. NHW, 0.794(0.666–1.157)]); 52-SNP IR GRS (NHB, 0.0095[0.009–0.010] and Hispanic, 0.0096 [0.0092–0.0101] vs. NHW, 0.0090[0.0084–0.0095]).</div></div><div><h3>Conclusions</h3><div>Impaired β-cell function may underlie T2D etiology more profoundly in NHB, whereas hepatic dysfunction, lipid metabolism abnormalities, and genetic IR contribute to T2D etiology to a greater degree in both NHB and Hispanics. Further validation of these findings may form the basis for a personalized medicine approach to prevention and treatment of T2D.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103128"},"PeriodicalIF":4.7,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strong correlation between double-strand DNA Breaks and total sperm DNA fragmentation in the human ejaculate","authors":"Jaime Gosálvez ,&nbsp;Stephen D. Johnston ,&nbsp;Ahinoa Prado ,&nbsp;Carmen López-Fernández ,&nbsp;Pablo Contreras ,&nbsp;Javier Bartolomé-Nebreda ,&nbsp;Mercedes González-Martínez ,&nbsp;José Luis Fernández ,&nbsp;Carlos García de la Vega ,&nbsp;Alfredo Góngora","doi":"10.1016/j.arcmed.2024.103122","DOIUrl":"10.1016/j.arcmed.2024.103122","url":null,"abstract":"<div><h3>Background</h3><div>Double- and single-strand DNA breaks (DSBs and SSBs, respectively) in spermatozoa, which emerge from intrinsic and extrinsic degenerative processes, are likely related to the underlying male pathology.</div></div><div><h3>Aim</h3><div>To determine whether the incidence of DSBs in the human ejaculate is a consistent predictor of whole sperm DNA fragmentation (W-SDF = SSBs + DSBs).</div></div><div><h3>Methods</h3><div>A correlation between the proportion of spermatozoa that showed whole W-SDF and those displaying only DSBs in DNA. Two patient cohorts were established: W-SDF ≤30% (low SDF; <em>n</em> = 153) and W-SDF ≥30% (high SDF; <em>n</em> = 222).</div></div><div><h3>Results</h3><div>An increasing level of W-SDF is associated with an increased incidence of DSBs in the ejaculate. When data from both the low and high W-SDF groups were combined, a linear relationship was observed, with DSBs increasing by 0.799 units for each unit increase in W-SDF. However, when the cohorts were analyzed separately, the relationships differed. In the low SDF group, DSBs increased linearly by 0.559 units for each unit increase in W-SDF. In the high SDF group, DSBs increased exponentially by 0.602 units per unit of W-SDF. Furthermore, the data dispersion between the two variables was significantly different between the cohorts, with the high SDF group showing 0.8 times greater variability than the low SDF group.</div></div><div><h3>Conclusions</h3><div>While the presence of DSBs in sperm is correlated with the W-SDF present in raw semen samples, the biological mechanisms responsible for DSBs are expressed in different proportions and/or at different levels in ejaculates with higher levels of DNA damage.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103122"},"PeriodicalIF":4.7,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}