Archives of Medical Research最新文献

{"title":"Membrane vesicles from lactobacillus acidophilus reduce intestinal inflammation and increase 5-HT in the substantia nigra of rats with parkinson's disease","authors":"Khadijeh Sheikh,&nbsp;Javad Arasteh,&nbsp;Maryam Tajabadi Ebrahimi,&nbsp;Ardeshir Hesampour","doi":"10.1016/j.arcmed.2024.103143","DOIUrl":"10.1016/j.arcmed.2024.103143","url":null,"abstract":"<div><h3>Background</h3><div>This study aimed to investigate the role of membrane vesicles (MVs) from the probiotic Lactobacillus acidophilus in reducing intestinal inflammation and increasing 5-hydroxytryptamine (5-HT) and tyrosine hydroxylase (TH) in the substantia nigra in the 6-hydroxydopamine (6-OHDA) rat model of Parkinson's disease (PD).</div></div><div><h3>Methods</h3><div>Twenty healthy male Wistar rats were randomly assigned to four groups (<em>n</em> = 5 per group), including a) control, b) 6-OHDA, c) 6-OHDA+MV, and d) sham groups. PD was induced by bilateral injection of 6-OHDA. Rats in the 6-OHDA+MV group received MV equivalent to 1 × 10⁷ colony-forming units (CFU)/mL 3 d/wk by oral gavage for 4 wk. At the end of 4 wk, all rats were sacrificed; the brain and small intestine were removed for cellular and molecular analysis.</div></div><div><h3>Results</h3><div>The induction of PD by 6-OHDA induced a remarkable decrease in beam-walking (<em>p</em> &lt; 0.0001). In addition, the expression of protein and genes (receptor) of 5-HT (r-5-HT1A) decreased, and that of protein and gene (receptor; GABBR1) of GABA increased in the PD group <em>(p</em> &lt; 0.05 compared with the healthy control group), while MV gavage of 6-OHDA-injected rats controlled these factors in the substantia nigra. In the intestinal tissue, the expression of TLR-4 and α-synuclein gene was significantly increased in the 6-OHDA group compared to the control group (<em>p</em> &lt; 0.0001).</div></div><div><h3>Conclusion</h3><div>MVs might act as potential beneficial tools to reduce intestinal inflammation, control neurological damage associated with PD, and increase 5-HT neurotransmitters. It seems that MVs from L. acidophilus may have therapeutic potential in Parkinson's neurological disorder by controlling the gut-brain axis.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103143"},"PeriodicalIF":4.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PAM-13 in clinical cancer care: A systematic review","authors":"Kenzy Jean Pierre ,&nbsp;Elise Verot ,&nbsp;Wafa Bouleftour","doi":"10.1016/j.arcmed.2024.103145","DOIUrl":"10.1016/j.arcmed.2024.103145","url":null,"abstract":"<div><h3>Background</h3><div>The Patient Activation Measure-13 (PAM-13) assesses patients’ ability to manage their healthcare. This systematic review aimed to summarize the relationship between the PAM-13 and patient characteristics, as well as program effectiveness.</div></div><div><h3>Methods</h3><div>A systematic literature search was conducted using the PubMed database and following the PRISMA guidelines. After screening and selection processes, a total of 21 articles were included for analysis.</div></div><div><h3>Results</h3><div>Half of single cancer type studies have been conducted on breast cancer. In terms of impact on the PAM-13 score, non-digital programs (75%) demonstrated higher efficacy in cancer care compared to digital ones (14.29%). Patient characteristics, including education, fear of recurrence, and time spent with healthcare providers, influenced activation levels.</div></div><div><h3>Conclusion</h3><div>This systematic review provides insights into the association of PAM-13 with patient characteristics and program effectiveness in clinical cancer care. Future research should validate interventions using PAM-13 and improve digital initiatives to optimize cancer care outcomes.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103145"},"PeriodicalIF":4.7,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142873741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Throughput RNA Sequencing of Peripheral Blood Mononuclear Cells Reveals Inflammatory State in Women with PCOS","authors":"Roozbeh Heidarzadehpilehrood ,&nbsp;Maryam Pirhoushiaran ,&nbsp;Malina Binti Osman ,&nbsp;King-Hwa Ling ,&nbsp;Habibah Abdul Hamid","doi":"10.1016/j.arcmed.2024.103129","DOIUrl":"10.1016/j.arcmed.2024.103129","url":null,"abstract":"<div><h3>Background</h3><div>Polycystic ovary syndrome (PCOS) is an endocrine and reproductive condition affecting women of reproductive age, although its expression profiles and molecular pathways are not fully understood.</div></div><div><h3>Aims</h3><div>To identify the transcriptome expression profiles of peripheral blood mononuclear cells (PBMCs) in women with PCOS and controls. To investigate noninvasive diagnostic biomarkers and potential treatment targets to improve women's fertility.</div></div><div><h3>Methods</h3><div>RNA sequencing (RNA-Seq) was conducted on PBMC samples from six patients with PCOS and six healthy controls. qRT-PCR validation was carried out in 68 subjects. Multivariate logistic regression was performed to assess the combined impact of biomarkers.</div></div><div><h3>Results</h3><div>A total of 186 differentially expressed genes (DEG) were found between patients and controls (log₂FC &gt;1, <em>p</em> &lt; 0.05). Enrichment analysis revealed cytokine-mediated signaling pathways, cytokine activity, and cytokine-cytokine receptor interaction. RNA sequencing showed consistency with qRT-PCR. Women with PCOS had significantly higher levels of <em>AQP9</em> (<em>p</em> &lt; 0.001), <em>PROK2</em> (<em>p</em> = 0.001), and <em>S100A12</em> (<em>p</em> &lt; 0.001) expression compared to controls. <em>AQP9</em> (AUC = 0.77), <em>PROK2</em> (AUC = 0.71), and <em>S100A12</em> (AUC = 0.82) adequately discriminated women with PCOS from healthy controls. In addition, multiple logistic regression on biomarkers resulted in a significant diagnostic power with an AUC = 0.89, 95 % CI: 0.81–0.97, <em>p</em> &lt; 0.0001. Further associations were analyzed between relative gene expression and clinical, anthropometric, hormonal, and ultrasonographic data.</div></div><div><h3>Conclusions</h3><div>Dysregulated RNA expression in PBMCs may contribute to an increased risk of PCOS and serve as a potential diagnostic biomarker. The involvement of inflammatory and cytokine-related pathways supports the notion that PCOS is a chronic inflammatory condition.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103129"},"PeriodicalIF":4.7,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preface Updating reproductive medicine","authors":"Victor S. Vital-Reyes ,&nbsp;J. Ricardo Loret De Mola","doi":"10.1016/j.arcmed.2024.103135","DOIUrl":"10.1016/j.arcmed.2024.103135","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103135"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial intelligence in human reproduction","authors":"Gerardo Mendizabal-Ruiz ,&nbsp;Omar Paredes ,&nbsp;Ángel Álvarez ,&nbsp;Fátima Acosta-Gómez ,&nbsp;Estefanía Hernández-Morales ,&nbsp;Josué González-Sandoval ,&nbsp;Celina Mendez-Zavala ,&nbsp;Ernesto Borrayo ,&nbsp;Alejandro Chavez-Badiola","doi":"10.1016/j.arcmed.2024.103131","DOIUrl":"10.1016/j.arcmed.2024.103131","url":null,"abstract":"<div><div>The use of artificial intelligence (AI) in human reproduction is a rapidly evolving field with both exciting possibilities and ethical considerations. This technology has the potential to improve success rates and reduce the emotional and financial burden of infertility. However, it also raises ethical and privacy concerns. This paper presents an overview of the current and potential applications of AI in human reproduction. It explores the use of AI in various aspects of reproductive medicine, including fertility tracking, assisted reproductive technologies, management of pregnancy complications, and laboratory automation. In addition, we discuss the need for robust ethical frameworks and regulations to ensure the responsible and equitable use of AI in reproductive medicine.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103131"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Study of variations in semen quality in men from 12 geographical locations in Spain","authors":"Rocío Núñez ,&nbsp;Andrés Guijarro ,&nbsp;Pablo Alberola ,&nbsp;Nuria Santamaría ,&nbsp;Mireia Poveda ,&nbsp;Ada Mora ,&nbsp;Marta Masip ,&nbsp;Sofía Sánchez ,&nbsp;Sara Alonso ,&nbsp;Teresa Rubio ,&nbsp;Inmaculada Barros ,&nbsp;Paloma González ,&nbsp;Sonia Gili ,&nbsp;I. Santiago Álvarez","doi":"10.1016/j.arcmed.2024.103140","DOIUrl":"10.1016/j.arcmed.2024.103140","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate possible variations in semen quality among patients undergoing fertility evaluation in 12 different geographical locations in Spain.</div></div><div><h3>Methods</h3><div>The study was conducted in 12 assisted reproduction centers located in different regions of Spain. Semen samples from 2,336 men seeking fertility assessment were analyzed. Seminal parameters—including semen volume, sperm concentration, motility, morphology, vitality, and total motile sperm count (TMS) were compared by geographic location. All parameters were evaluated using standardized methodologies, with interlaboratory quality controls to ensure consistency.</div></div><div><h3>Results</h3><div>No significant differences in patient age were found between centers (ANOVA, <em>p</em> &gt; 0.05). However, statistically significant variations in semen volume, sperm concentration, total motility, and TMS were observed between the centers (<em>p</em> = 0.020, 0.004, 0.000, and 0.008, respectively). Men from Asturias exhibited the highest values for sperm concentration (mean: 59.8 ± 48.7 × 10⁶ sperm/mL), motility (mean total motility: 54.3 ± 20.7%), and TMS (mean: 101.2 ± 107.5 × 10^6), with statistically significant differences compared to other regions. Patients from Cataluña, Almería, and Málaga followed in these metrics. In contrast, men from Granada presented the lowest sperm concentration and TMS (mean concentration: 43.1 ± 35.8 × 10^6 sperm/mL; mean TMS: 43.1 ± 34.6 × 10^6), followed by individuals from Alicante and Madrid. No significant differences in sperm morphology or vitality were observed between centers.</div></div><div><h3>Conclusion</h3><div>Since all seminal parameters were assessed using standardized methodologies, the observed differences in semen quality between regions are unlikely to be due to laboratory variability.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103140"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical and Laboratorial Evaluation of Male Infertility. A Detailed Practical Approach","authors":"Lucas G. Fraga ,&nbsp;João PM. Gismondi ,&nbsp;Lucas V. Sanvido ,&nbsp;Ana Flávia Q. Lozano ,&nbsp;Thiago A. Teixeira ,&nbsp;Jorge Hallak","doi":"10.1016/j.arcmed.2024.103139","DOIUrl":"10.1016/j.arcmed.2024.103139","url":null,"abstract":"<div><div>Infertility, defined as the inability to achieve pregnancy after one year of unprotected, frequent sexual intercourse, is a global burden that affects approximately 15% of couples, or 190–230 million people worldwide, who are trying to start a family. The male contributes significantly, directly accounting for 30–35% and up to 52% of total couple infertility, affecting approximately 7–8% of all men. This work aims to present, in a didactic and objective way, a standardization of the initial steps toward a thorough evaluation of subfertile and infertile men. We have focused on the evaluation of initial management, emphasizing the need for a comprehensive evaluation that includes a detailed history, physical examination, and semen analysis as the golden triple helix of basic evaluation of the infertile male. General and genital physical examinations are highly valuable due to the wealth of information they provide, from potential diagnoses to pregnancy prognoses. Comprehensive and quality-controlled semen analysis provides reliable information as a baseline test to evaluate the patency of the reproductive tract and to evaluate basic sperm parameters and fertility potential. However, it is not a fertility determinant and should preferentially be complemented with sperm functional tests. like biomarkers of oxidative stress, sperm immaturity and DNA fragmentation. Most cases of infertility require evaluation by a specialist in andrology, nonetheless the understanding and rationale of the initial assessment of the infertile male can be undertaken by non-specialists, thus improving the care and counseling of couples facing this troubling issue and avoiding unnecessary use of assisted reproductive technologies (ART) since most cases of male infertility can be treated and reversed by medical or surgical interventions, and the fertility status can be restored. The ultimate goal is to achieve natural pregnancy, the use of ART should not be the initial offered resource.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103139"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792880","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive medicine: The future is now","authors":"Sergio Papier ,&nbsp;Fiamma Di Biase ,&nbsp;Julieta Quaglia","doi":"10.1016/j.arcmed.2024.103138","DOIUrl":"10.1016/j.arcmed.2024.103138","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103138"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental outcomes of very preterm infants conceived through in vitro fertilization","authors":"Taylor Badger ,&nbsp;Emma Johns ,&nbsp;Teresa Wilson ,&nbsp;Kathleen Groesch ,&nbsp;Paula Diaz-Sylvester ,&nbsp;Erin Bauer ,&nbsp;Albert Botchway ,&nbsp;Melanie Nielsen ,&nbsp;Julio Ricardo Loret de Mola ,&nbsp;Beau Batton","doi":"10.1016/j.arcmed.2024.103136","DOIUrl":"10.1016/j.arcmed.2024.103136","url":null,"abstract":"<div><h3>Objective</h3><div>To compare in-hospital and neurodevelopmental (ND) outcomes between very preterm infants conceived spontaneously and those conceived utilizing <em>in vitro</em> fertilization (IVF).</div></div><div><h3>Study Design</h3><div>A 10-year retrospective study of a large regional, mixed urban and rural population was conducted. IVF conceived infants born &lt;32 0/7 weeks gestational age (GA) were matched 1:2 to control patients who did not undergo any documented assisted reproductive technology (ART) based on GA, birth weight and gender. In-hospital and ND data were analyzed.</div></div><div><h3>Results</h3><div>There were 35,712 live births, including 755 (2.1%) IVF-conceived births (519 [68.7%] full term, 236 [31.3%] preterm, 33 born &lt;32 0/7 weeks GA [14.0%] – including 27 cared for in the HSHS St. John's Hospital NICU with long-term ND data). When compared to 54 controls, these 27 very preterm IVF-conceived infants were more likely to be born to older women (OR 1.21 [1.07–1.35], <em>p</em> = 0.001), but other demographic data (i.e., including primigravida status, length of hospital stay, use of antenatal corticosteroids, mode of delivery, rural residency, etc.) were similar. Compared to other very preterm neonates, IVF conceived very preterm infants were less likely to receive surfactant therapy (9 [33%] vs. 34 [63%], <em>p</em> = 0.104). Other in-hospital outcomes were similar. Data for ND outcomes through 24 months (non-matched data due to loss to follow-up) were also similar between cohorts.</div></div><div><h3>Conclusion</h3><div>Infants born very preterm after IVF conception have similar in-hospital and ND outcomes when compared to other very preterm infants. This is reassuring information that can be shared with individuals considering IVF conception.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"55 8","pages":"Article 103136"},"PeriodicalIF":4.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical characteristics and outcomes of infective endocarditis in patients with cancer: A multicenter case-control study","authors":"Gustavo Mendez-Hirata ,&nbsp;Frida Rivera-Buendía ,&nbsp;Eduardo Viveros-Rentería ,&nbsp;Eric Ochoa-Hein ,&nbsp;Patricia Cornejo-Juárez ,&nbsp;Manlio Márquez ,&nbsp;José Ramón Hidalgo-Dura ,&nbsp;Yoana Leyva-López ,&nbsp;Diana Vilar-Compte","doi":"10.1016/j.arcmed.2024.103123","DOIUrl":"10.1016/j.arcmed.2024.103123","url":null,"abstract":"<div><h3>Background</h3><div>Due to increased exposure to health hazards, patients with cancer are at a higher risk of developing infective endocarditis (IE), which in turn maximizes their risk of poor outcomes. This study aimed to analyze IE events in patients with cancer and matched controls.</div></div><div><h3>Methods</h3><div>We conducted a retrospective case-control study in four third-level centers in Mexico City between 2006 and 2022. Patients with IE were identified; cases (patients with cancer) were matched in a 1:2 ratio with controls (patients without cancer). Baseline characteristics, factors associated with IE, and outcomes were assessed in both groups.</div></div><div><h3>Results</h3><div>A total of 108 patients were included (36 cases and 72 controls). After logistic regression analysis, the risk factors independently associated with IE in cases were obesity (adjusted odds ratio [aOR] 9.03, 95 % CI 1.5–51.8), surgery within six months before IE (aOR 6.23, 95 % CI 1.8–21.5), and invasive procedures within six months prior to IE (aOR 3.89, 95 % CI 1.15–13.1). Healthcare-associated IE was more common in these cases, as were systemic embolic episodes. Subjects with <em>S. aureus</em> IE were more prone to experience systemic embolization. There were no differences in mortality between the groups.</div></div><div><h3>Conclusion</h3><div>In this study, risk factors associated with the healthcare environment were more frequent in patients with IE and cancer. <em>S. aureus</em> was a common culprit and was associated with systemic embolization.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103123"},"PeriodicalIF":4.7,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142744274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}