Archives of Medical Research最新文献

{"title":"Phase Angle/C-reactive Protein-Index as a Novel Combined Tool for Predicting Liver-Related Hospitalizations in MASLD-Decompensated Cirrhosis","authors":"Marcello Dallio ,&nbsp;Mario Romeo ,&nbsp;Annachiara Coppola,&nbsp;Giuseppina Martinelli,&nbsp;Claudio Basile,&nbsp;Fiammetta Di Nardo,&nbsp;Carmine Napolitano,&nbsp;Paolo Vaia,&nbsp;Alessia De Gregorio,&nbsp;Alessia Silvestrin,&nbsp;Giusy Senese,&nbsp;Alessandro Federico","doi":"10.1016/j.arcmed.2025.103306","DOIUrl":"10.1016/j.arcmed.2025.103306","url":null,"abstract":"<div><h3>Background</h3><div>Phase angle (<em>PA</em>), a bioelectrical impedance analysis (BIA)-assessable marker of sarcopenia, can accurately predict hospitalizations in advanced chronic liver disease (ACLD). <em>PA</em> has never been specifically investigated in metabolic dysfunction-associated steatotic liver disease (MASLD). Sarcopenia is commonly observed in patients with MASLD, whereas individuals with compensated ACLD (cACLD)-MASLD progress more rapidly to decompensated ACLD (dACLD).</div></div><div><h3>Aims</h3><div>To evaluate the <em>PA</em> values across the different stages of liver disease progression in MASLD, and to estimate the accuracy of the novel <em>PA</em>/C-reactive-protein (CRP) index (PAC-I) in predicting 3 month further liver-related events (LREs) and hospitalizations in MASLD-dACLD.</div></div><div><h3>Methods</h3><div>Patients with MASLD were consecutively enrolled and stratified according to liver stiffness measurement (LSM) and eventual previous LREs in ≤F3 fibrosis (AF), cACLD, and dACLD. At baseline, anthropobiochemical, nutritional, and clinical data were collected. Patients with MASLD-dACLD were followed up, and further LRE-determining hospitalizations within the subsequent three months were recorded.</div></div><div><h3>Results</h3><div>In patients with MASLD, <em>PA</em> values were inversely correlated with LSM, and progressively decreased according to the worsening of liver disease (cACLD vs. dACLD, <em>p</em> &lt;0.0001). In patients with dACLD, <em>PA</em> inversely correlated with CRP levels. PAC-I showed greater accuracy and superiority compared to <em>PA</em> alone in predicting the occurrence of further LREs within three months (PAC-I &lt;2.35: optimal cut-off). Consistently, patients with dACLD with PAC-I &lt;2.35 presented a higher risk of further LREs.</div></div><div><h3>Conclusions</h3><div>In MASLD, PA correlates with disease progression status. PAC-I represents a useful noninvasive tool in predicting short-term liver-related hospitalizations in dACLD.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 8","pages":"Article 103306"},"PeriodicalIF":3.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sleep as a Mediator: Decoding the Complex Relationship Between Coffee, Exercise, and Semen quality","authors":"Yu Liu ,&nbsp;Wenrui Lu ,&nbsp;Guanxiang Yuan ,&nbsp;Qi Zhou ,&nbsp;Benhong Xu ,&nbsp;Jianjun Liu ,&nbsp;Peiyi Liu","doi":"10.1016/j.arcmed.2025.103317","DOIUrl":"10.1016/j.arcmed.2025.103317","url":null,"abstract":"<div><h3>Objectives</h3><div>Given the global decline in semen quality, it is crucial to investigate the modifiable factors, such as lifestyle that influence semen quality in men. This cross-sectional observational study evaluated the association between coffee intake, physical activity, sleep, and semen quality.</div></div><div><h3>Methods</h3><div>A total of 3,302 male participants, aged 18–60 years, underwent urological assessments, provided semen samples, and participated in anthropometric measurements and a questionnaire addressing lifestyle factors related to semen quality. A multiple linear regression model was used to examine the associations between coffee consumption, physical activity, sleep, and semen quality. Furthermore, mediation models were developed to explore the potential mediating role of sleep in the relationship between coffee intake, physical activity, and semen quality.</div></div><div><h3>Results</h3><div>The results showed that not drinking coffee was positively associated with the sperm total motility rate, and the sperm progressive motility rate, and negatively associated with the sperm immotility rate. Physical activity was also positively correlated with semen volume. Subgroup analysis showed that physical activity mitigated the effect of coffee on semen quality. In addition, mediation analysis revealed that the time of falling asleep mediated the relationship between coffee consumption and semen quality. Moreover, the average duration of nightly sleep mediated the relationship between physical activity and various semen motility parameters.</div></div><div><h3>Conclusions</h3><div>The associations among coffee intake, physical activity, sleep and semen quality were statistically significant. These findings highlight the importance of a healthy lifestyle for optimal male semen quality.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 3","pages":"Article 103317"},"PeriodicalIF":3.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145093073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Controlled Ovarian Stimulation Contributes to the Incidence of de Novo Chromosomal Abnormalities in Cleavage-Stage Embryos","authors":"Yinghui Ye ,&nbsp;Jieliang Ma ,&nbsp;Qitao Zhan ,&nbsp;Ruimin Zhao ,&nbsp;Xinyun Yang ,&nbsp;Yangyun Zou ,&nbsp;Fan Jin","doi":"10.1016/j.arcmed.2025.103318","DOIUrl":"10.1016/j.arcmed.2025.103318","url":null,"abstract":"<div><h3>Background</h3><div>Studies on the effects of ovarian stimulation on the embryo aneuploidy rate have shown conflicting results.</div></div><div><h3>Objectives</h3><div>To investigate whether parameters of controlled ovarian stimulation (COS) are associated with the incidence of <em>de novo</em> chromosomal abnormalities in cleavage-stage embryos undergoing preimplantation genetic testing (PGT).</div></div><div><h3>Methods</h3><div>This retrospective study examined data from biopsies of cleavage-stage embryos with one blastomere from the Women’s Hospital at Zhejiang University School of Medicine. <em>De novo</em> chromosomal abnormalities were classified as: <em>de novo</em> chromosomal segmental abnormalities (DCSAs) or <em>de novo</em> whole chromosomal abnormalities (DWCAs). A multivariable logistic regression model was used to identify factors that may affect the genomic stability of embryos. Further stratification analysis was performed to explore the impact of these factors on DCSAs and DWCAs.</div></div><div><h3>Results</h3><div>A total of 1,994 cleavage-stage biopsied embryos were included in the analysis. The number of DWCAs but not DCSAs increased significantly with maternal age. The incidence of <em>de novo</em> chromosomal abnormalities was similar between different COS protocols. Longer stimulation duration was associated with a considerably higher DWCA rate (<em>p</em> = 0.003). The DCSA rate increased with higher gonadotropin dosages. Furthermore, cotreatment with growth hormone (GH) supplementation was associated with a significantly lower incidence of DWCAs (<em>p</em> = 0.006).</div></div><div><h3>Conclusion</h3><div>Ovarian stimulation parameters are related to the incidence of <em>de novo</em> chromosomal abnormalities in preimplantation embryos. GH supplementation had a beneficial effect in decreasing aneuploidy, and higher gonadotropin doses may be related to mitotic errors.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 3","pages":"Article 103318"},"PeriodicalIF":3.4,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145092836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation of Intestinal Permeability in Health and Disease: Possible Therapeutic Applications","authors":"Azmavet M. Güemes-González ,&nbsp;Lourdes A. Arriaga-Pizano ,&nbsp;Rommel Chacón-Salinas ,&nbsp;Isabel Wong-Baeza ,&nbsp;Eduardo Ferat-Osorio","doi":"10.1016/j.arcmed.2025.103321","DOIUrl":"10.1016/j.arcmed.2025.103321","url":null,"abstract":"<div><div>Intestinal permeability is the process by which certain substances pass through the intestinal barrier and enter the bloodstream to reach the tissues and organs where they are needed. This vital process is regulated by various physiological mechanisms, depending on the type of molecule being translocated, from ions and water molecules to large peptides and vitamins. However, under certain conditions, intestinal permeability can increase, exposing the body to the passage of unwanted molecules and pathogenic microorganisms. The various mechanisms involved in increased intestinal permeability include the zonulin pathway, intestinal epithelial cell death, intestinal dysbiosis, and transcytosis. Each of these mechanisms is activated by certain stimuli, and may occur in isolation or together. The translocation of molecules and pathogens has been linked to the development or worsening of certain pathologies, such as acute pancreatitis, sepsis, inflammatory bowel diseases, irritable bowel syndrome, celiac disease, systemic lupus erythematosus, obesity, depression, and schizophrenia. This is mainly due to the inflammatory response generated in response to the translocated microorganisms. Research has been conducted to develop therapies to decrease intestinal permeability in several diseases. This review summarizes knowledge related to pathways that regulate intestinal permeability, diseases associated with increased intestinal permeability, and advances in potential treatments.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 3","pages":"Article 103321"},"PeriodicalIF":3.4,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracellular Galectin-3 as a Crucial Regulator of Foam Cell Formation and Apoptosis Progression through the Modulation of Membrane Lipid Rafts","authors":"Wen-cheng Liu ,&nbsp;Chin-sheng Lin ,&nbsp;Chen-ling Luo ,&nbsp;Weng-yu Yang ,&nbsp;Cheng-hung Yang ,&nbsp;Mei-Ling Cheng ,&nbsp;Min-chien Tsai ,&nbsp;Wei-shiang Lin ,&nbsp;Hao-ai Shui ,&nbsp;Yi-ping Chuang","doi":"10.1016/j.arcmed.2025.103300","DOIUrl":"10.1016/j.arcmed.2025.103300","url":null,"abstract":"<div><h3>Background</h3><div>Atherosclerosis, a leading cause of cardiovascular disease (CVD) mortality worldwide, is characterized by dysregulated lipid metabolism and unresolved inflammation. Macrophage-derived foam cell formation and apoptosis contribute to plaque formation and vulnerability. Elevated serum galectin-3 (Gal-3) levels are associated with increased CVD risk, and Gal-3 in plaques is strongly associated with macrophages. However, its impact on inflammation remains controversial.</div></div><div><h3>Methods</h3><div>An <em>in vitro</em> model using human THP-1-derived macrophages was established to simulate inflammatory foam cells. CRISPR-Cas9-assisted LGALS3 knockout in THP-1 cells was performed to elucidate the functional role of Gal-3 in inflammation and apoptosis.</div></div><div><h3>Results</h3><div>OxLDL-internalized THP-1-derived foam cells secreted significant Gal-3 upon lipopolysaccharide (LPS) treatment, mimicking elevated Gal-3 levels observed in patients with CVD. LPS-treated foam cells showed Gal-3-dependent inflammation, endoplasmic reticulum stress, and apoptosis progression. LGALS3 knockout reduced oxLDL uptake and inflammation, accompanied by decreased surface expression of CD36 and CD14. This indicates a disrupted lipid raft structure, as confirmed by reduced cholera toxin B subunit recognition and altered sphingolipid profile. Gal-3 inhibitors GB1107 and TD139 attenuated LPS-induced cytokine production. Notably, only GB1107 inhibited oxLDL uptake by partially disrupting lipid raft organization, due to its superior membrane permeability. GB1107 further altered Gal-3 nuclear localization under LPS or oxLDL exposure, suggesting a potential nuclear role during inflammation. Importantly, GB1107 reduced foam cell formation in primary human monocyte-derived macrophages, supporting its translational relevance.</div></div><div><h3>Conclusions</h3><div>Gal-3 modulates lipid raft organization and oxLDL internalization, acting as a pro-inflammatory mediator in foam cell biology. These findings highlight Gal-3 as a potential therapeutic target for early atherosclerosis.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 2","pages":"Article 103300"},"PeriodicalIF":3.4,"publicationDate":"2025-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145027431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maternal Phthalate Exposure Alters Prostate Proteome in Rat Offspring: Linking Omics Insights to Prostate Cancer Risk in Humans","authors":"Patrick Vieira Souza ,&nbsp;Ariana Musa Aquino ,&nbsp;Luiz Guilherme Alonso-Costa ,&nbsp;Natália Magosso ,&nbsp;Marcos Antonio Fernandes de Oliveira ,&nbsp;Vanessa Aguiar Rocha ,&nbsp;Matheus Naia Fioretto ,&nbsp;Mirella Franco Moreira ,&nbsp;Victória Cristina Pinha ,&nbsp;Gabriel Henrique Caxali ,&nbsp;Luis Antônio Justulin ,&nbsp;Jodi Anne Flaws ,&nbsp;Wellerson Rodrigo Scarano","doi":"10.1016/j.arcmed.2025.103297","DOIUrl":"10.1016/j.arcmed.2025.103297","url":null,"abstract":"<div><h3>Background</h3><div>Phthalates are compounds used as plasticizers to increase the flexibility of plastics and are considered endocrine disruptors. Some studies suggest that the origin of prostate cancer (PCa) may be associated with disturbances during embryo-fetal development. Previous data showed that perinatal exposure to the same phthalate mixture (PM) used here increased the incidence of adenocarcinomas in the prostates of aged rats. Building on our earlier work, this study identifies proteins altered in the prostate proteome by exposure to a PM during gestation and lactation in rats, focusing on proteins in the human secretome and their correlation with PCa.</div></div><div><h3>Methods</h3><div>Pregnant SD rats were divided into three groups and treated from gestational day (GD)10 to postnatal day (PND)21. On PND22 the differentially abundant proteins in the offspring’s prostate were compared with the predicted secreted proteins in humans. Then, the abundance of selected proteins was compared among groups and enriched. Finally, a protein-protein interaction network was obtained. The resulting data were cross-referenced with data for PCa and some targets were validated by RT-qPCR and Western blot.</div></div><div><h3>Results</h3><div>Perinatal exposure to PM affected the endoplasmic reticulum, decreasing the amount of certain proteins crucial for protein folding and secretion, impairing secretion of several proteins important for proper prostate development. Furthermore, <em>in silico</em> analysis revealed that several proteins in the rat proteome are also altered in patients with PCa.</div></div><div><h3>Conclusions</h3><div>Our results suggest that early exposure to phthalates may modulate protein secretion, creating a microenvironment that impairs tissue development and increases susceptibility to oncogenesis.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 2","pages":"Article 103297"},"PeriodicalIF":3.4,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145011099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uterus Transplantation: State of the Art in and Arrent Status in Mexico","authors":"Luis Eduardo Magdaleno-Marquez ,&nbsp;Sonny John Stetson ,&nbsp;Aldo Loza Mapomé ,&nbsp;Julio César Del Hierro Yepo ,&nbsp;Juan Carlos Cantón Romero ,&nbsp;Edwin Ramirez ,&nbsp;Federico Mendoza Sánchez ,&nbsp;Diego Federico Mendoza Medina ,&nbsp;Roberto Enrique Díaz González ,&nbsp;Martha Isolina García Amador ,&nbsp;Luis Arturo Ruvalcaba-Castellon","doi":"10.1016/j.arcmed.2025.103280","DOIUrl":"10.1016/j.arcmed.2025.103280","url":null,"abstract":"<div><div>This study examines uterine transplantation (UTx) in 2024. It focuses on various aspects, such as animal models, clinical outcomes, surgical procedures, patient perspectives, and ethical and legal considerations. The study particularly considers the context of Mexico. We also explore collaborative initiatives and the public health implications of this innovative reproductive advancement technology.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 1","pages":"Article 103280"},"PeriodicalIF":3.4,"publicationDate":"2025-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145010565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response to Comment on: Evaluation of Multimorbidity Burden in Frailty Transitions in Costa Rican Older Adults Using Multistate Markov Models","authors":"RAFAEL OGAZ-GONZÁLEZ ,&nbsp;QIAN ZOU ,&nbsp;LUIS MIGUEL GUTIÉRREZ-ROBLEDO ,&nbsp;MALAQUÍAS LÓPEZ-CERVANTES ,&nbsp;EVA CORPELEIJN","doi":"10.1016/j.arcmed.2025.103299","DOIUrl":"10.1016/j.arcmed.2025.103299","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 2","pages":"Article 103299"},"PeriodicalIF":3.4,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotics for the Management of Liver Cirrhosis and Its Complications","authors":"Junxiu Chen ,&nbsp;Haonan Zhao ,&nbsp;Nahum Méndez-Sánchez ,&nbsp;Xingshun Qi","doi":"10.1016/j.arcmed.2025.103304","DOIUrl":"10.1016/j.arcmed.2025.103304","url":null,"abstract":"<div><div>Cirrhosis is the terminal stage of various chronic liver diseases, and its decompensated stage is mainly characterized by serious complications, such as hepatic encephalopathy, ascites, spontaneous bacterial peritonitis, and gastrointestinal bleeding. Gut microbial dysbiosis is prevalent in patients with cirrhosis. Considering the bidirectional regulation of the gut-liver axis, dysbiosis is closely related to the development and progression of liver cirrhosis. Probiotics, which modulate the gut microbiome, have received significant attention for managing cirrhosis and its complications. Through a review of relevant literature, we have provided a comprehensive summary of the potential efficacy of probiotics in treating cirrhosis and its complications, providing new insights into targeting the gut microbiome.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 8","pages":"Article 103304"},"PeriodicalIF":3.4,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144996325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long chain ceramides promote Anxiety-like behavior and microglia activation in female mice","authors":"Sofía Bernal-Vega ,&nbsp;Gabriela Cruz-Carrillo ,&nbsp;Orlando Flores-Maldonado ,&nbsp;Miguel Becerril-García ,&nbsp;Juan Carlos Corona-Castillo ,&nbsp;Alberto Camacho-Morales","doi":"10.1016/j.arcmed.2025.103303","DOIUrl":"10.1016/j.arcmed.2025.103303","url":null,"abstract":"<div><h3>Background</h3><div>Long-chain ceramides have been implicated in anxiety-like behavior and in priming microglial activation, suggesting a possible lipid–immune crosstalk in emotional regulation.</div></div><div><h3>Methods</h3><div>We systemically administered a mixture of C16:0, C18:0, C22:0, C24:0, and C24:1 ceramides to adult male and female mice. Anxiety-like behavior was assessed with behavioral tests. Microglial phenotypes in the cortex, hippocampus, and striatum were evaluated via flow cytometry. Regression models examined associations between behavior and immune markers. <em>In vitro</em> assays assessed phagocytosis, gene expression, mitochondrial potential, and ROS production in microglia after ceramide exposure.</div></div><div><h3>Results</h3><div>Female, but not male, mice showed anxiety-like behavior after ceramide treatment. CD86+ microglia increased in the cortex, while CD206+ microglia decreased across brain regions. Behavioral scores correlated with CD86+ cells in cortex and CD206+ cells in hippocampus. <em>In vitro</em>, ceramides enhanced phagocytosis and induced NLRP3 and TNF-α expression. Ceramides also reduced mitochondrial potential and increased ROS in microglia.</div></div><div><h3>Conclusions</h3><div>Ceramide administration was associated with anxiety-like behavior in female mice, along with changes in microglial activation. These effects appear to be associated with mitochondrial dysfunction and oxidative stress, suggesting a lipid–immune interaction that could contribute to anxiety vulnerability.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"57 2","pages":"Article 103303"},"PeriodicalIF":3.4,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144997470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}