Archives of Medical Research最新文献

{"title":"Assessing Hypertension Management in Peru's Peri-Urban Community in the Wake of the COVID-19 Pandemic","authors":"Laura Espinoza-Pajuelo ,&nbsp;Patricia J. García ,&nbsp;Jesús Medina-Ranilla ,&nbsp;Antonio Bernabé-Ortiz ,&nbsp;Ezequiel García-Elorrio ,&nbsp;Hannah H. Leslie","doi":"10.1016/j.arcmed.2024.103156","DOIUrl":"10.1016/j.arcmed.2024.103156","url":null,"abstract":"<div><h3>Background</h3><div>Hypertension is a global health challenge, particularly in low- and middle-income countries. Peri-urban areas such as Pampas de San Juan de Miraflores, Peru, face challenges that the COVID-19 pandemic exacerbated.</div></div><div><h3>Objective</h3><div>To assess the post-COVID-19 hypertension care cascade and mean systolic blood pressure (SBP) levels in Pampas de San Juan de Miraflores and to determine the proportion of individuals with hypertension who are diagnosed, treated, and have controlled blood pressure across age groups.</div></div><div><h3>Methods</h3><div>A cross-sectional survey (October 2022–January 2023) including adults aged &gt;35 years from the 2010 CRONICAS study and a geographically based sample of households nearby. Data collection included a hypertension awareness questionnaire and standardized blood pressure measurements. Descriptive analyses characterized the age-specific hypertension care cascade and calculated mean blood pressure in four groups: healthy, unaware, aware and untreated, and aware and treated.</div></div><div><h3>Results</h3><div>Among 2,856 adults, age-specific hypertension prevalence based on self-reported medication use and current blood pressure screening was 13.3 % (35–44 years) to 54.6 % (&gt;75 years). Awareness was 85 %; of those, 85 % were treated, and 76.8 % of them had controlled blood pressure. Mean SBP was highest in the unaware group (<em>n</em> = 102, 146.0 mmHg) and lowest in the healthy group (<em>n</em> = 1,929, 111.0 mmHg). These values were comparable among diagnosed patients, whether treated (<em>n</em> = 333, 126.0 mmHg) or untreated (<em>n</em> = 492, 128.0 mmHg).</div></div><div><h3>Conclusions</h3><div>Despite high awareness and relatively adequate control among untreated individuals, a substantial proportion remain undiagnosed or untreated after COVID-19. These findings underscore the need for interventions to improve early detection, treatment, and follow-up of hypertension.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 4","pages":"Article 103156"},"PeriodicalIF":4.7,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143043950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on “Features and Allele Frequency of JAK2 exon 12-mutated Polycythemia Vera in Comparison with JAK2 V617F-mutated Disease”","authors":"Stephen E. Langabeer","doi":"10.1016/j.arcmed.2024.103180","DOIUrl":"10.1016/j.arcmed.2024.103180","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 4","pages":"Article 103180"},"PeriodicalIF":4.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient and reproducible differentiation protocol for pluripotent stem cells into functional endothelial cells: Unveiling the path to vascular regeneration","authors":"Manizheh Azhdari ,&nbsp;Axel zur Hausen ,&nbsp;Nasser Aghdami ,&nbsp;Mohamadreza Baghaban-Eslaminejad","doi":"10.1016/j.arcmed.2024.103142","DOIUrl":"10.1016/j.arcmed.2024.103142","url":null,"abstract":"<div><h3>Introduction</h3><div>Endothelial cells (ECs) play a crucial role in many treatments for cardiovascular diseases, such as blood vessel repair, tissue engineering, and drug delivery. The process of differentiating these cells is complex and involves various sources and numerous molecular and cellular events. Differentiating pluripotent stem cells (PSCs) into endothelial cells are one of the most effective sources for creating ECs in the lab and offers great potential for regenerative medicine. However, different cell types can appear during differentiation process.</div></div><div><h3>Objectives</h3><div>This study presents a reliable and reproducible protocol for efficiently differentiating human pluripotent stem cells (hPSCs) into mature endothelial cells with high purity (&gt;98%).</div></div><div><h3>Methods</h3><div>FLK1⁺ cells were isolated from hPSCs using fluorescence-activated cell sorting (FACS). Then isolated FLK1⁺ cells differentiated into high-purity endothelial cells (ECs) by adding endothelial growth factors (VEGF, FGF, and EGM-2 medium). The differentiated ECs were extensively characterized by evaluating key endothelial markers and assessing their functional abilities, such as tube formation and response to angiogenic signals. Finally, the ECs were further purified using a second FACS step with a CD31 antibody.</div></div><div><h3>Results</h3><div>The differentiated hPSC-derived endothelial cells (hPSC-ECs) expressed high levels of PECAM-1 (CD31), VE-cadherin (CD144), and von Willebrand factor (vWF), with more than 98% of the cells showing these markers. Additionally, the hPSC-ECs formed tubular structures and effectively took up acetylated fluorescently-labeled low-density lipoprotein (DiI-ac-LDL), demonstrating their functionality as endothelial cells.</div></div><div><h3>Conclusion</h3><div>Our study clarifies the molecular mechanisms involved in the differentiation of hPSCs into endothelial cells, emphasizing key signaling pathways important for determining endothelial cell fate. These findings provide a framework for the scalable production of transplantable endothelial cells, representing a significant advancement in personalized therapies and tissue engineering for regenerative medicine.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103142"},"PeriodicalIF":4.7,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NHERF2 regulatory function in signal transduction pathways and control of gene expression: Implications for cellular homeostasis and breast cancer","authors":"Adrián-Alejandro Paredes-Villa ,&nbsp;Isaac Esaú Aguilar-Arce ,&nbsp;Iván Meneses-Morales ,&nbsp;Rafael Cervantes-Roldán ,&nbsp;Viviana Valadéz-Graham ,&nbsp;Alfonso León-Del-Río","doi":"10.1016/j.arcmed.2024.103179","DOIUrl":"10.1016/j.arcmed.2024.103179","url":null,"abstract":"<div><div>Na⁺/H⁺ exchanger regulatory factor 2 (NHERF2) is a nucleocytoplasmic protein initially identified as a regulator of membrane-bound sodium-hydrogen exchanger 3 (NHE3). In the cytoplasm, NHERF2 regulates the activity of G protein-coupled receptors (GPCRs), including beta-2 adrenergic receptor (2β-AR), lysophosphatidic acid receptor 2, and parathyroid hormone type 1 receptor. In the nucleus, NHERF2 acts as a coregulator of transcription factors such as sex-determining region Y protein (SRY), involved in male sex determination, and estrogen receptor alpha (ERα). ERα is a ligand-dependent transcription factor that controls mammary gland growth and differentiation during puberty and pregnancy and plays a major role in the development and progression of breast cancer tumors. Altogether, the regulatory functions of NHERF2 on ion channels, GPCRs, and nuclear transcription factors have a modulatory effect on signal transduction pathways, metabolic homeostasis, cell proliferation and differentiation, neurotransmission, muscle contraction, and renal electrolyte balance. This work highlights NHERF2 functions in the cytoplasm and nucleus and underscores the nuclear mechanisms through which NHERF2 participates in the regulation of gene expression and tumor growth and progression in breast cancer.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 4","pages":"Article 103179"},"PeriodicalIF":4.7,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fibroblast plasticity in pulmonary and cardiovascular fibrosis: Mechanistic insights and emerging therapeutic targets","authors":"Hector A. Cabrera-Fuentes ,&nbsp;Guillermo Barreto ,&nbsp;Ebtesam Abdullah Al-Suhaimi ,&nbsp;Elisa A. Liehn","doi":"10.1016/j.arcmed.2024.103173","DOIUrl":"10.1016/j.arcmed.2024.103173","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 4","pages":"Article 103173"},"PeriodicalIF":4.7,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mutational Profiling of Korean Lymphomas and Diffuse Large B-Cell Lymphoma Subtype Classification Using Targeted Panel Sequencing","authors":"Gilsung Yoo ,&nbsp;Jooyoung Cho ,&nbsp;Hyeong Ju Kwon ,&nbsp;Taesic Lee ,&nbsp;JinJu Kim ,&nbsp;Juwon Kim","doi":"10.1016/j.arcmed.2024.103158","DOIUrl":"10.1016/j.arcmed.2024.103158","url":null,"abstract":"<div><h3>Background</h3><div>Lymphoma is a common hematological malignancy with diverse morphological and immunophenotypic characteristics that may affect treatment and outcomes. Thus, accurate differential diagnosis is crucial, and molecular genetic testing is valuable. We aimed to investigate the genetic characteristics of Korean patients with lymphoma using a next-generation sequencing (NGS)-based targeted panel.</div></div><div><h3>Methods</h3><div>We designed a panel covering 588 genes clinically and diagnostically relevant in lymphoma through a literature review and performed targeted panel sequencing of 34 formalin-fixed paraffin-embedded samples from patients with lymphoma.</div></div><div><h3>Results</h3><div>Among the 34 samples examined, diffuse large B-cell lymphoma (DLBCL) was the most common, followed by NK/T-cell lymphoma, peripheral T-cell lymphoma, anaplastic large cell lymphoma, T-lymphoblastic leukemia/lymphoma, MALT lymphoma, and mantle cell lymphoma. The genes <em>TP53, MYD88, CD79B, FOXO1, KMT2D, MYD88, TNFRSF14</em>, and <em>TET2</em> had the highest number of mutations. For patients with DLBCL, we used modified genetic subtype classification algorithms from previous studies and successfully classified 31.3–50 % of them into specific subtypes. Notably, four patients were allocated a distinct subtype across all algorithms.</div></div><div><h3>Conclusions</h3><div>Our targeted panel sequencing can effectively reveal lymphoma mutational profiles of samples in clinical settings and partially outline the mutational landscape of lymphomas in the Korean population, particularly DLBCL.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 4","pages":"Article 103158"},"PeriodicalIF":4.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143178832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Humoral immune response to SARS-CoV-2 vaccines in patients with autoimmune rheumatic diseases","authors":"Paula María Corbalan ,&nbsp;Rodrigo Hernán Tomas-Grau ,&nbsp;Mariana Pera ,&nbsp;Diego Ploper ,&nbsp;Gabriela Vanesa Espasa ,&nbsp;Silvia Inés Cazorla ,&nbsp;María Lilia Leguizamón ,&nbsp;Esteban Vera Pingitore ,&nbsp;Ana Lucía Barbaglia ,&nbsp;Carolina Maldonado-Galdeano ,&nbsp;María Constanza Bertolaccini ,&nbsp;Silvana Estefanía Soliz-Santander ,&nbsp;Luciana González Lucero ,&nbsp;César Luis Ávila ,&nbsp;Rosana Nieves Chehín ,&nbsp;Héctor Raúl Sueldo ,&nbsp;Sergio Benjamín Socias ,&nbsp;Verónica Inés Bellomio","doi":"10.1016/j.arcmed.2024.103141","DOIUrl":"10.1016/j.arcmed.2024.103141","url":null,"abstract":"<div><h3>Background</h3><div>Patients with autoimmune rheumatic diseases (ARD) are at increased risk of infection due to their impaired immune response, which also reduces vaccination efficacy. Although several studies have evaluated the serological response to SARS-CoV-2 mRNA-based vaccines in patients with ARD, limited information on immune responses to other vaccination platforms is available.</div></div><div><h3>Aims</h3><div>This observational prospective study aims to investigate the humoral immune response to different SARS-CoV-2 vaccines in patients with ARD.</div></div><div><h3>Methods</h3><div>Total 66 patients with ARD who were scheduled to receive any SARS-CoV-2 vaccine (Gam-COVID-Vac; AZD1222; BBIBP-CorV; mRNA-1273; BNT162b2 and Ad5-nCoV) were enrolled in the study. We analyzed the humoral immune response elicited against the spike receptor-binding-domain (RBD) of SARS-CoV-2 at 0 and 14 ± 2 d after the first vaccine dose and at 0 ± 1, 21–45, and 180 d after the second one. Titers were also measured in patients who received an additional dose of vaccine.</div></div><div><h3>Results</h3><div>After the second dose of the vaccine, 70.5 % experienced seroconversion. The type of vaccine affected serological responses. BBIBP-CorV resulted in lower seroconversion rates, while mixed vaccinations increased anti-RBD titers. Other factors impacting seroconversion were higher prednisone doses, biological therapy, and hypertension. Patients treated with Rituximab had the lowest seroconversion rate. Regression analysis revealed an 89.0 % lower probability of seroconversion for BBIBP-CorV recipients and an 88.0 % lower probability for those with hypertension. An additional dose increased seroconversion to 85.7 %.</div></div><div><h3>Conclusions</h3><div>Two-dose vaccination schemes exhibited a 70.5 % seroconversion rate to the SARS-CoV-2 vaccine. An additional dose increased this rate to 85.0 %. Reduced humoral immune responses were associated with BBIBP-CorV, prednisone higher doses, and biological therapy.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 3","pages":"Article 103141"},"PeriodicalIF":4.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143173320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of 5 G mobile phone network electromagnetic field exposure on testicular endoplasmic reticulum stress and the protective role of coenzyme Q10","authors":"Hamit Yilmaz ,&nbsp;Levent Tümkaya ,&nbsp;Tolga Mercantepe ,&nbsp;Adnan Yılmaz ,&nbsp;Fatih Gül ,&nbsp;Zehra Topal Suzan","doi":"10.1016/j.arcmed.2024.103157","DOIUrl":"10.1016/j.arcmed.2024.103157","url":null,"abstract":"<div><h3>Aim</h3><div>Nowadays, the electromagnetic field (EMF) has become an issue of <em>electromagnetic pollution</em>. This study aimed to determine the effect of 5 G Fr1 frequency band EMF waves on endoplasmic reticulum (ER) stress in testicular tissue and to demonstrate the efficacy of coenzyme Q10 (CoQ10) in suppressing the potential situation.</div></div><div><h3>Materials and Methods</h3><div>Three groups of eight male Sprague-Dawley rats were established. In group I (control) received only saline solution by oral gavage. In group II (EMF), saline solution was administered by oral gavage and the rats were exposed to 5.9 GHz EMF for 2 h/d (09:00–11:00). In group III (EMF+ CoQ10), the EMF exposure was created in the same way as in group II and CoQ10 was given daily at 10 mg/kg (CoQ10), 2 mg/kg dissolved in corn oil and administered by oral gavage for the experimental period (30 days).</div></div><div><h3>Results</h3><div>Histopathological damage, including edematous regions, vascular congestion, and neutrophil inflammation accompanied by loss of spermatogenic cells, particularly spermatozoa, and spermatids, increased thiobarbituric acid (TBARS) and decreased total thiol (TT) levels were observed in the seminiferous tubules in the EMF group. Furthermore, germinal epithelial cells showing intense GRP-78 immunopositivity and spermatogenic cells indicating C/EBP homologous protein (CHOP) immunopositivity were increased in the seminiferous tubules. On the other hand, in the EMF+CoQ10 group, both histopathological and biochemical findings were observed in the opposite direction.</div></div><div><h3>Conclusion</h3><div>Due to its antioxidant properties and its ability to suppress oxidative stress and apoptosis, CoQ10 may be a promising candidate against 5 G EMF-induced testicular ER damage.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 4","pages":"Article 103157"},"PeriodicalIF":4.7,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143179117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviewers list","authors":"","doi":"10.1016/S0188-4409(24)00220-0","DOIUrl":"10.1016/S0188-4409(24)00220-0","url":null,"abstract":"","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 1","pages":"Article 103170"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143159715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"You Are as Old as the Connectivity You Keep: Distinct Neurophysiological Mechanisms Underlying Age-Related Changes in Hand Dexterity and Strength","authors":"Emilio Chiappini ,&nbsp;Sonia Turrini ,&nbsp;Francesca Fiori ,&nbsp;Mariagrazia Benassi ,&nbsp;Alessia Tessari ,&nbsp;Giuseppe di Pellegrino ,&nbsp;Alessio Avenanti","doi":"10.1016/j.arcmed.2024.103031","DOIUrl":"10.1016/j.arcmed.2024.103031","url":null,"abstract":"<div><h3>Background</h3><div>Aging can lead to a decline in motor control. While age-related motor impairments have been documented, the underlying changes in cortico-cortical interactions remain poorly understood.</div></div><div><h3>Methods</h3><div>We took advantage of the high temporal resolution of dual-site transcranial magnetic stimulation (dsTMS) to investigate how communication between higher-order rostral premotor regions and the primary motor cortex (M1) influences motor control in young and elderly adults. We assessed the dynamics of connectivity from the inferior frontal gyrus (IFG) or pre-supplementary motor area (preSMA) to M1, by testing how conditioning of the IFG/preSMA affected the amplitude of motor evoked potentials (MEPs) induced by M1 stimulation at different temporal intervals. Moreover, we explored how age-related changes in premotor-M1 interactions relate to motor performance.</div></div><div><h3>Results</h3><div>Our results show that both young and elderly adults had excitatory IFG-M1 and preSMA-M1 interactions, but the two groups’ timing and strength differed. In young adults, IFG-M1 interactions were early and time-specific (8 ms), whereas in older individuals, they were delayed and more prolonged (12-16 ms). PreSMA-M1 interactions emerged early (6 ms) and peaked at 10-12 ms in young individuals but were attenuated in older individuals. Critically, a connectivity profile of the IFG-M1 circuit like that of the young cohort predicted better dexterity in older individuals, while preserved preSMA-M1 interactions predicted greater strength, suggesting that age-related motor decline is associated with specific changes in premotor-motor networks.</div></div><div><h3>Conclusions</h3><div>Preserving youthful motor network connectivity in older individuals is related to maintaining motor performance and providing information for interventions targeting aging effects on behavior.</div></div>","PeriodicalId":8318,"journal":{"name":"Archives of Medical Research","volume":"56 1","pages":"Article 103031"},"PeriodicalIF":4.7,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}