Archives of oral biology最新文献

{"title":"NFIC activates DEPTOR and blocks mTOR signaling to inhibit glycolysis and immune escape in oral squamous cell carcinoma","authors":"Jian Xu ,&nbsp;Mohang Sun ,&nbsp;Xin Wang ,&nbsp;Yuxin Zou ,&nbsp;Lina Wang","doi":"10.1016/j.archoralbio.2025.106442","DOIUrl":"10.1016/j.archoralbio.2025.106442","url":null,"abstract":"<div><h3>Objective</h3><div>Oral squamous cell carcinoma (OSCC) has a poor prognosis and high mortality. This study aims to investigate the potential molecular mechanisms of NFIC/DEPTOR/mTOR signaling in glycolysis and tumor immune escape in OSCC.</div></div><div><h3>Design</h3><div><em>In vitro</em>, the effects of the NFIC/DEPTOR/mTOR axis on the proliferative capacity and apoptosis of HN30 and SCC-25 cells were investigated using colony formation assays, EdU staining, and TUNEL after lentiviral gene interference. The glycolytic activity of OSCC cells was analyzed through glucose uptake, ECAR, and lactate production. CD8⁺ T cells were co-cultured with OSCC cells to analyze the cytotoxic activity of CD8⁺ T cells. SCC-25 cells with different genetic interventions were injected subcutaneously into NOG mice to investigate the effects of NFIC/DEPTOR/mTOR on subcutaneous tumor growth, tumor microenvironment (TME) acidity, and CD8⁺ T cell infiltration and activity. ChIP-qPCR and dual-luciferase assays were used to investigate the regulatory relationship between NFIC and the DEPTOR promoter in OSCC cells.</div></div><div><h3>Results</h3><div>NFIC and DEPTOR were significantly downregulated in OSCC cells and tissues, accompanied by enhanced mTOR signaling. NFIC activated DEPTOR by binding to its promoter. Overexpression of either NFIC or DEPTOR significantly inhibited OSCC cell proliferation, glycolytic activity, and enhanced CD8⁺ T cell killing capacity. Similarly, they inhibited tumor growth <em>in vivo</em>, blocked TME acidification, and weakened tumor immune escape. Combined DEPTOR knockdown rescued the malignant progression of OSCC inhibited by NFIC overexpression.</div></div><div><h3>Conclusion</h3><div>NFIC mediates DEPTOR transcription to repress mTOR signaling, thereby hindering OSCC progression by suppressing cell proliferation, glycolytic activity, and CD8⁺ T cell-related immune escape.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106442"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145425205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TWF1 promotes tumor progression in head and neck squamous cell carcinoma via AKT phosphorylation","authors":"Yingshun Yang ,&nbsp;Kaicheng Yang ,&nbsp;Shixiong Peng ,&nbsp;Shasha Man ,&nbsp;He Chen","doi":"10.1016/j.archoralbio.2025.106425","DOIUrl":"10.1016/j.archoralbio.2025.106425","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the molecular mechanisms underlying how Twinfilin actin-binding protein 1 (TWF1) drives the malignant progression of head and neck squamous cell carcinoma (HNSCC).</div></div><div><h3>Design</h3><div>A pan-cancer analysis of TCGA datasets was performed to evaluate <em>TWF1</em> expression and prognostic significance, followed by validation using quantitative real-time PCR (qPCR) and immunohistochemistry (IHC) in clinical HNSCC specimens. Functional assays (CCK-8, colony formation, wound healing, and Transwell invasion) were conducted following siRNA-mediated TWF1 knockdown in SCC9 cells. Additionally, the same functional assays were conducted in an HSC3 cell model overexpressing TWF1 to further evaluate its oncogenic function. The involvement of the AKT signaling pathway was examined by Western blot and validated through rescue experiments with the AKT activator SC79.</div></div><div><h3>Results</h3><div><em>TWF1</em> was found to be upregulated in 13 cancer types, including HNSCC. Elevated <em>TWF1</em> expression, advanced T stage, and lymph node metastasis were identified as independent prognostic indicators in HNSCC. qPCR confirmed increased <em>TWF1</em> expression, and IHC showed higher TWF1 protein levels in HNSCC tissues. TWF1 knockdown suppressed SCC9 cell proliferation, migration, and invasion, and reduced phosphorylated AKT levels. Conversely, TWF1 overexpression in HSC3 cells promoted proliferation, migration, and invasion while enhancing AKT phosphorylation. SC79 treatment partially reversed the malignant phenotypes suppressed by TWF1 knockdown, supporting TWF1’s regulatory role through AKT phosphorylation.</div></div><div><h3>Conclusion</h3><div>TWF1 acts as an oncogenic driver in HNSCC, promoting tumor progression through the AKT signaling pathway. It may serve as a potential therapeutic target in precision medicine for HNSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106425"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular vesicles derived from Filifactor alocis induce interleukin-6 production in osteoblasts via Toll-like receptor 2 signaling","authors":"Yuma Tsuruta ,&nbsp;Tongxin Liu ,&nbsp;Haruna Yokoi ,&nbsp;Kentaro Maruyama ,&nbsp;Eiji Nemoto ,&nbsp;Takashi Nishioka ,&nbsp;Kenji Matsushita ,&nbsp;Hiroyuki Tada","doi":"10.1016/j.archoralbio.2025.106443","DOIUrl":"10.1016/j.archoralbio.2025.106443","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effect of the periodontal bacterium <em>Filifactor alocis</em> on proinflammatory cytokine production by osteoblasts. Specifically, we examined the mechanisms underlying interleukin (IL)-6 production by osteoblasts stimulated with live and lyophilized <em>F. alocis</em>, and <em>F. alocis</em>-derived extracellular vesicles (EVs).</div></div><div><h3>Design</h3><div>MC3T3-E1 mouse osteoblasts were stimulated with live bacteria of <em>F. alocis</em> and, as a comparative control, <em>P. gingivalis</em>. Furthermore, cells were treated with <em>F. alocis</em> lyophilized whole cells or EVs, and IL-6 production was quantified by ELISA; cytokine expression profiled using membrane-based array; and mRNA expression analyzed by RT-qPCR. Signaling pathways were analyzed using specific inhibitors. Statistical analyses were performed using non-parametric tests with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div><em>F. alocis</em> live bacteria, lyophilized whole cells, and <em>F. alocis</em>-derived EVs induced IL-6, leukemia inhibitory factor, and chemokines in MC3T3-E1 cells. Mechanistically, IL-6 production by <em>F. alocis</em> involved osteoblast activation via Toll-like receptor (TLR) 2 on the bacterial surface or released EVs. Lyophilized <em>F. alocis</em> induced IκB-ζ mRNA expression in osteoblasts. Moreover, <em>F. alocis</em>-derived EVs induced IL-6 production in osteoblasts via protein kinase C (PKC).</div></div><div><h3>Conclusions</h3><div>Live and lyophilized <em>F. alocis</em> and <em>F. alocis</em>-derived EVs induce the production of proinflammatory cytokines by osteoblasts, including IL-6. Additionally, the induction of IL-6 by <em>F. alocis</em>-derived EVs involves the activation of TLR2 and PKC. Conclusively, these findings suggest that <em>F. alocis</em>-induced cytokine production by osteoblasts may induce osteoclast differentiation, highlighting its role in the pathogenesis of periodontitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106443"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomechanical impact of tooth root morphology to inform dental implant design","authors":"Amber P. Wood-Bailey ,&nbsp;Chris Smith ,&nbsp;Laura C. Fitton ,&nbsp;Alana C. Sharp","doi":"10.1016/j.archoralbio.2025.106455","DOIUrl":"10.1016/j.archoralbio.2025.106455","url":null,"abstract":"<div><h3>Objective</h3><div>Using finite element analysis (FEA), this study aims to investigate the impact of different tooth root morphologies and implant designs, including a standard implant and a custom root-analogue implant on stress and strain distribution across the mandible.</div></div><div><h3>Design</h3><div>Six models were created by varying the root morphology of one tooth (the mandibular first molar) under identical loading scenarios: an original molar root, an incisor root, a canine root, a taurodont root, a standard implant, and a custom root-analogue implant replicating the original root morphology.</div></div><div><h3>Results</h3><div>Models with the original molar and custom implant exhibited similar stress and strain distributions over the mandible and had higher principal strains (tensile and compressive) compared to the single-rooted and standard implant models. Specifically, the maximum tensile and compressive strain values in the mandible of the custom implant model reach 94.89 % and 99.15 % of those in the original tooth root model. In contrast, the other models show less than 55.68 % similarity.</div></div><div><h3>Conclusions</h3><div>Custom root-analogue implants, which mimic natural root morphology, demonstrated more favourable stress distribution patterns, similar to those of the natural molar, compared to single-root implants. Our findings suggest that multi-rooted teeth are biomechanically optimized for dissipating masticatory loads, and standard single-root implants may not adequately replicate these properties, leading to poor load distribution and increased failure risk in posterior locations. Further research is needed to refine custom root-analogue implant designs and optimize their clinical application to better match the natural biomechanical environment of the maxilla and mandible.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106455"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145497716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Action mechanisms of progesterone in managing bone loss in periodontitis: An in vitro study","authors":"Ying Man ,&nbsp;Shengjie Yan ,&nbsp;Jianyong Qin,&nbsp;Xiaofei Li,&nbsp;Hongliang Qiu,&nbsp;Xinyue Zhang","doi":"10.1016/j.archoralbio.2025.106436","DOIUrl":"10.1016/j.archoralbio.2025.106436","url":null,"abstract":"<div><h3>Objective</h3><div>Progesterone (PG) is a sex steroid hormone that has the potential to control bone loss in periodontitis. This study aimed to reveal the underlying mechanisms of action of PG on osteogenesis in periodontitis and to identify key target genes <em>in vitro</em>.</div></div><div><h3>Design</h3><div>Primary human periodontal mesenchymal stem cells (hPDLSCs) were isolated and treated with lipopolysaccharide (LPS) in combination with or without PG. Subsequently, RNA sequencing was performed after osteogenic induction, and the differential expressed genes (DEGs), functional enrichment (GO and KEGG), and protein-protein interaction (PPI) were bioinformatically analysed. The expression of two key DEGs, BUB1 and UBE2C, were verified by qRT-PCR. Relevant regulatory mechanisms involving osteogenesis were verified by detecting the levels of osteogenesis-related proteins, Runx2 and ALP (Western blot) after silencing.</div></div><div><h3>Results</h3><div>A total of 212 DEGs (104 up-regulated and 108 down-regulated genes) were identified between the LPS and LPS + PG groups. These DEGs were mainly enriched in the GO terms of cell division, adhesion, sodium/potassium channel, etc., and in the KEGG terms of oocyte meiosis, estrogen signalling, cell apoptosis, and adhesion. In the PPI networks, most of the hub proteins are related to cell cycle and division. Of note, BUB1 and UBE2C, two node genes, were down-regulated in LPS-treated hPDLSCs. The intervention of PG significantly eliminated the effects of LPS on the down-regulation of BUB1 and UBE2C. Upon LPS treatment, silencing of BUB1 and UBE2C both reversed the PG-induced osteogenesis in hPDLSCs.</div></div><div><h3>Conclusions</h3><div>BUB1 and UBE2C, two important mediators of osteogenesis, are potential molecular targets of PG. PG may promote the osteogenesis of hPDLSCs under inflammatory conditions through the up-regulation of BUB1 and UBE2C, suggesting a potential mechanism that may contribute to the remission of periodontitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106436"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The microRNA-34b-3p/SOX5 axis regulates cellular activities and tumor angiogenesis in oral squamous cell carcinoma","authors":"Mingke Huang ,&nbsp;Yu Ren ,&nbsp;Jiwen Zheng ,&nbsp;Yang Cao ,&nbsp;Yu Zhu ,&nbsp;Jun Zhou ,&nbsp;Zhuo Ling ,&nbsp;Guoyu Xiong ,&nbsp;Hangyu Zhou","doi":"10.1016/j.archoralbio.2025.106438","DOIUrl":"10.1016/j.archoralbio.2025.106438","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to ascertain the significance of microRNA-34b-3p (miR-34b-3p) in the prognosis and tumor angiogenesis associated with the development of oral squamous cell carcinoma (OSCC).</div></div><div><h3>Design</h3><div>The relative abundances of miR-34b-3p and tumor angiogenesis-associated genes were quantified using quantitative real-time polymerase chain reaction. Cellular events were monitored using the Cell Counting Kit-8 and Transwell assays. Additionally, a luciferase reporter assay was carried out to verify the interaction between miR-34b-3p and Sex Determining Region Y-box Protein 5 (SOX5).</div></div><div><h3>Results</h3><div>MiR-34b-3p was significantly decreased in tissue samples from OSCC patients in comparison to healthy individuals (<em>P</em> &lt; 0.001) and displayed a robust diagnostic accuracy in distinguishing OSCC patients from healthy controls. Moreover, OSCC patients expressing low miR-34b-3p levels displayed a short overall survival time (<em>P</em> = 0.002). Additionally, low miR-34b-3p expression was an independent predictor for the clinical outcome of OSCC patients (<em>P</em> = 0.011, HR=0.447, 95 %CI=0.239–0.834). Through biological experiments, downregulation of miR-34b-3p could promote cell proliferation, migration and invasion of OSCC cells. Notably, following transfection miR-34b-3p inhibitor, VEGFA and MMP9 mRNA expressions were dramatically enhanced (<em>P</em> &lt; 0.001). SOX5 was identified as a potential target of miR-34b-3p. Recovery experiments verified that the knockdown of SOX5 counteracted the impacts of miR-34b-3p on cellular activities and angiogenesis.</div></div><div><h3>Conclusions</h3><div>Downregulated miR-34b-3p expression promoted OSCC progression by enhancing cellular activities and upregulating the expression of pro-angiogenic factors via targeting SOX5.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106438"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145474891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of head and neck radiotherapy doses on apical periodontitis progression: An animal-based study","authors":"Gustavo Guimarães Guerrero ,&nbsp;Giovanna B. Minhoto ,&nbsp;Camilla dos S. Tibúrcio-Machado ,&nbsp;Rayana D. Khoury ,&nbsp;Carolina O. Lima ,&nbsp;Emmanuel J.N.L. Silva ,&nbsp;Claudio A. Federico ,&nbsp;Marcia C. Valera","doi":"10.1016/j.archoralbio.2025.106422","DOIUrl":"10.1016/j.archoralbio.2025.106422","url":null,"abstract":"<div><h3>Aim</h3><div>The aim of this study was to assess the dose-dependent effects of radiotherapy on the progression of apical periodontitis (AP) in rats.</div></div><div><h3>Methods</h3><div>Forty-five animals were divided into five groups based on radiation dose and apical periodontitis induction (n = 9): AP-RT7 (irradiated with a dose of 7.5 Gy + AP); AP-RT10 (10 Gy dose + AP); AP-RT15 (15 Gy dose + AP); AP (AP without radiation), and Control (no intervention). Radiation therapy was administered on the first day of the experiment. After seven days, the apical periodontitis induction was carried out in all groups except Control. All animals were euthanized after 21 days of AP progression (day 28), and the area and volume of AP were analyzed using radiography, micro-CT, and histological analyses. Inflammation intensity and extent were assessed histologically. Statistical analysis was performed using ANOVA and Student's t test for quantitative data, and Kruskal-Wallis for ordinal data (P &lt; 0.05).</div></div><div><h3>Results</h3><div>The AP-RT15 group exhibited significantly larger apical periodontitis lesions compared to the AP-RT7.5, AP-RT10, and AP groups, as demonstrated by radiographic (p &lt; 0.05), micro-CT (p &lt; 0.0001), and histological analyses (p &lt; 0.0001). Histological examination further revealed a more extensive and intense inflammatory response in the AP-RT15 group (p &lt; 0.01). Overall, radiotherapy contributed to increased apical bone resorption and exacerbated inflammation in a dose-dependent manner.</div></div><div><h3>Conclusions</h3><div>The progression of apical periodontitis in irradiated animals follows a dose-dependent pattern, with higher radiation doses markedly amplifying the lesion's area, volume, and inflammatory severity.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106422"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elaeodendron buchananii (Loes.) Loes. stem bark: Unveiling the phytochemical profiles and selective bioactivity to combat tongue cancer","authors":"Abhay Prakash Mishra ,&nbsp;Anyapat Atipimonpat ,&nbsp;Manisha Nigam ,&nbsp;Masande Yalo ,&nbsp;Sudarshan Singh ,&nbsp;Chuda Chittasupho ,&nbsp;Jennifer Nambooze ,&nbsp;Jarunya Ngamkham ,&nbsp;Neti Waranuch","doi":"10.1016/j.archoralbio.2025.106434","DOIUrl":"10.1016/j.archoralbio.2025.106434","url":null,"abstract":"<div><h3>Objective</h3><div>The study aimed to investigate the cytotoxic and anti-inflammatory potential of the methanolic stem bark extract of <em>Elaeodendron buchananii</em> (EB) through <em>in vitro</em> assays and LC-MS based phytochemical profiling.</div></div><div><h3>Design</h3><div>The cytotoxicity of EB was evaluated using the MTT assay on cell lines: RAW 264.7 (murine macrophages), HGF-1 (human gingival fibroblasts), and SCC-25 (human tongue squamous carcinoma cells). Oxidative stress and membrane damage were evaluated using reactive oxygen species (ROS) and lactate dehydrogenase (LDH) assays, respectively, while susceptibility to oxidative stress was examined through H₂O₂ challenge. A Griess reagent assay was used to determine EB's anti-inflammatory properties in LPS-stimulated RAW 264.7 cells.</div></div><div><h3>Results</h3><div>EB exhibited no toxicity to HGF-1 across a concentration range of 50–800 µg/mL, while showing moderate cytotoxicity against RAW 264.7 cells, at 1000 µg/mL (cell viability 67.63 ± 2.81 %). Notably, EB demonstrated selective cytotoxicity against SCC-25 cells (IC₅₀ = 581.9 µg/mL). Additionally, a significant reduction in nitric oxide (NO) production by EB was observed against RAW 264.7 cells when stimulated with LPS, indicating anti-inflammatory (IC₅₀ = 401.40 µg/mL) properties. LC-MS analysis identified 16 phytochemicals, including various secondary metabolites (such as ursolic acid, elaeodendroside F, and oleandrin), each recognized for their pharmacological relevance.</div></div><div><h3>Conclusion</h3><div>The findings demonstrate that EB possesses selective cytotoxicity toward tongue carcinoma cells and exhibits significant anti-inflammatory effects. These findings support its traditional medical application and point to its potential for additional phytopharmaceutical development. This report is the first to explore the <em>in vitro</em> cytotoxic effects of <em>E. buchananii</em> on tongue cancer cells.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106434"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exercise modulates local inflammation and tissue loss in experimental periodontitis: A histomorphometric and immunohistochemical study in ra","authors":"Gümrükçüoğlu Zehra ,&nbsp;Burcu Özkan Çetinkaya ,&nbsp;Ferda Pamuk Özer ,&nbsp;Sinem İnal ,&nbsp;Sevda Kurt Bayrakdar ,&nbsp;Muhammed Taha Kaya","doi":"10.1016/j.archoralbio.2025.106435","DOIUrl":"10.1016/j.archoralbio.2025.106435","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effects of resistance exercise on the progression of periodontal disease in rats, focusing on alveolar bone loss, attachment loss, and local inflammatory and bone metabolic markers.</div></div><div><h3>Materials and methods</h3><div>Forty male <em>Wistar albino</em> rats were allocated into four experimental groups in a 2 × 2 factorial design: G1 or G2 and G3 or G4. The exercise protocol consisted of progressive swimming sessions over eight weeks, with resistance provided by weights tied to the animals. In the final week, MBT was performed, and rats were sacrificed for histomorphometric and immunohistochemical analysis<strong>.</strong></div></div><div><h3>Results</h3><div>The values for alveolar bone loss and attachment loss were significantly lower in the exercised groups compared to both the G1 and G2 groups (p &lt; 0.001). Among the rats with periodontitis, TNF-α expression was significantly lower in the exercised group (p = 0.002), whereas IL-10 levels showed no significant differences (p &gt; 0.005). RANKL expression was elevated in G2 (p = 0.039), with no significant variation in OPG among groups. In MBT, G2 showed significantly higher values compared to G1 and G3 (p = 0.004). G4 displayed intermediate values, higher than G3 but lower than G2, without statistical significance (p &gt; 0.005).</div></div><div><h3>Conclusion</h3><div>Physical exercise reduced TNF-α levels in the presence of periodontitis and increased IL-10, although the latter change was not statistically significant. Exercise also contributed to a decrease in RANKL/OPG expression, suggesting a protective effect on alveolar bone resorption. MBT findings further indicate that exercise may alleviate periodontitis-associated behavioral alterations, supporting its potential role as an adjunctive therapy in managing periodontitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106435"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MIR375 hypermethylation as a prognostic predictor for head and neck squamous cell carcinoma: Insights from The Cancer Genome Atlas (TCGA)","authors":"Ana Carolina da Silva Lima ,&nbsp;Ileana Gabriela Sanghez de Rubió ,&nbsp;Shuting Lin ,&nbsp;Carla M. Prado ,&nbsp;Peng Qiu ,&nbsp;Maria Aderuza Horst","doi":"10.1016/j.archoralbio.2025.106437","DOIUrl":"10.1016/j.archoralbio.2025.106437","url":null,"abstract":"<div><h3>Objective</h3><div>To conduct exploratory bioinformatics analyses of the potential prognostic value of DNA methylation patterns in <em>MIR375</em> regulatory regions and of expression levels of its encoded microRNA, hsa‑miR‑375 (miR-375), in head and neck squamous cell carcinoma (HNSCC).</div></div><div><h3>Design</h3><div>Data from 490 HNSCC samples were obtained from The Cancer Genome Atlas (TCGA), 50 of which were paired non-tumor surgical margins. Clinicopathological data, DNA methylation data from regions associated with <em>MIR375</em>, and miR-375 expression data were analyzed. Kaplan-Meier curves and Cox regression analyses were used to determine overall survival (OS) and disease-free survival (DFS) at 5 years in each group.</div></div><div><h3>Results</h3><div>Differentially methylated regions were associated with miR-375 expression in HNSCC. Specially, hypermethylation in N shore region was independently associated with poor OS and DFS. After adjusting for methylation patterns in other regions and miR-375 expression, these associations remained significant. DFS was also associated with methylation patterns in the enhancer region. After adjusting for confounding factors (the most robust model), hypermethylation in the N shore region was found to be an unfavorable predictor of OS and DFS. Clinicopathological and lifestyle factors also influenced the OS and DFS rates.</div></div><div><h3>Conclusions</h3><div>Methylation in DNA regions that may influence <em>MIR375</em> transcription can be considered as predictors of OS and DFS in HNSCC, particularly those in the N shore region. This region should be a target for future investigations into <em>MIR375</em> methylation pattern and its role in modulating miR-375 expression in HNSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106437"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145380230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}