Archives of oral biology最新文献

{"title":"Three-dimensional analysis of lip and chin movements during mastication with artificial nasal obstruction","authors":"Yuichi Ariyasu,&nbsp;Yusuke Ban,&nbsp;Emi Inada,&nbsp;Daisuke Murakami,&nbsp;Haruyoshi Yamaza","doi":"10.1016/j.archoralbio.2025.106181","DOIUrl":"10.1016/j.archoralbio.2025.106181","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effects of nasal obstruction on the coordinated movement of perioral tissues during mastication using a motion capture system.</div></div><div><h3>Design</h3><div>Twelve healthy adult participants were instructed to chew gum only on their habitual masticatory side for 30 s, with and without nasal obstruction. Nasal obstruction was induced by blocking nasal breathing with a nose clip. Movements of the upper and lower lips, left and right corners of the mouth, and chin during gum chewing were measured using a motion capture system, and the differences between participants with and without nasal obstruction were compared. Multilevel linear model analysis was used to evaluate inter-individual and intra-individual variations in lip and chin movements, both with and without nasal obstruction.</div></div><div><h3>Results</h3><div>The chin chewing cycle duration was significantly longer in participants with nasal obstruction. The chewing cycle distance of the upper lip was significantly shorter in participants with nasal obstruction. The intra-individual variation in the chewing cycle distance of the chin and lips increased with nasal obstruction, whereas the inter-individual variation decreased.</div></div><div><h3>Conclusions</h3><div>Nasal obstruction causes unstable yet uniform movements of the chin and lips during mastication, while also restricting upper-lip movement. Our results indicate that nasal obstruction may significantly affect mandibular and lip movements during mastication. These findings highlight the importance of nasal breathing in maintaining stable perioral movements during mastication. This study provides new insights that could inform clinical strategies for managing oral function in individuals with nasal obstruction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106181"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next-generation sequencing analysis of bacterial species present in the sequestrum of medication-related osteonecrosis of the jaw patients","authors":"Na Rae Choi ,&nbsp;Hee Sam Na ,&nbsp;Hyejung Han ,&nbsp;Jin Chung ,&nbsp;Yong-Deok Kim","doi":"10.1016/j.archoralbio.2025.106180","DOIUrl":"10.1016/j.archoralbio.2025.106180","url":null,"abstract":"<div><h3>Objective</h3><div>Antiresorptives cases of adverse reactions to highly effective drugs used to treat skeletal-related events that occur during treatment of osteoporosis or malignancy have been reported in the jaw. To date, there is no clear pathophysiology for medication-related osteonecrosis of the jaw (MRONJ), but the role of inflammation or infection has been proposed as one of several possible pathogenesis theories. This study was designed to investigate the role of infection in the pathogenesis of MRONJ by analyzing the microbial communities observed in the bone samples from MRONJ patients.</div></div><div><h3>Design</h3><div>Oral microbial samples were collected from 39 MRONJ patients and 45 healthy patients and subjected to next generation sequencing analysis. Alpha diversity, beta diversity, linear discriminant analysis and effect size, and network analysis were applied to compare the microbiome features.</div></div><div><h3>Results</h3><div>The 10 predominant microbial strains observed in MRONJ patients were Streptococcus spp. S. constellatus, Tannerella forsythia, Parvimonas micra, Granulicatella adiacens, Bacteroidaceae G-1 bacterium HMT 272, Treponema socranskii, T. maltophilum, Pyramidobacter piscolens, and Fretibacterium fastidiosum. Network analysis revealed a significant correlation between T. forsythia and P. gingivalis. T. socranskii was closely related to T. maltophilum, Fretibacterium fastidiosum, and Peptostreptococcaceae nodatum. G. adiacens was closely related to Streptococcus parasanguinis, S. sanguinis, and Prevotella buccae.</div></div><div><h3>Conclusions</h3><div>In conclusion, this study sheds light on the potential role of infection in the pathogenesis of MRONJ. A distinct microbial profile was identified in MRONJ patients, with notable predominance of S. constellatus, T. forsythia, P. micra and G. adiacens among others. Network analysis further revealed significant correlations between certain microbial strains, suggesting possible synergistic interactions in MRONJ pathogenesis. These findings contribute to a deeper understanding of the complex microbial dynamics underlying MRONJ and may inform future therapeutic strategies aimed at targeting specific bacterial species implicated in this condition.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106180"},"PeriodicalIF":2.2,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of tooth-specific optical properties for the development of a non-invasive pulp diagnostic system using Transmitted-light plethysmography: An in vitro study","authors":"Hiroaki Ohki,&nbsp;Satoko Kakino,&nbsp;Tsutomu Iwamoto","doi":"10.1016/j.archoralbio.2025.106178","DOIUrl":"10.1016/j.archoralbio.2025.106178","url":null,"abstract":"<div><h3>Objectives</h3><div>Transmitted-light plethysmography (TLP) is an objective and non-invasive pulp diagnosis method that has already been validated for applications for incisors. However, there is a demand for TLP use in the molars, it has not yet been established for this application. This study investigated the optimal light source wavelengths for TLP in premolars, to establish a pulp diagnosis system based on measuring pulpal blood flow.</div></div><div><h3>Design</h3><div>One extracted incisor and one extracted premolar, which were fully developed and healthy, were prepared. The optical properties of model teeth filled with 0–30 % hematocrit contents in the pulp chamber were analyzed at 525, 590, and 625 nm wavelengths. The incident and transmitted light intensity of model teeth were measured to determine the optical density (O.D.) using a prototype plethysmograph (J.Morita) and a spectrometer. The significant differences in O.D. at each wavelength were analyzed using the Kruskal-Wallis test followed by the Steel-Dwass test as a post-hoc test. Light propagation through the teeth was also observed under a microscope.</div></div><div><h3>Results</h3><div>A statistically significant differences in O.D. were observed among the three wavelengths at all hematocrit concentrations (p &lt; 0.05). The observation of light absorption and scattering in the whole teeth supported the optical measurement results.</div></div><div><h3>Conclusion</h3><div>The results indicated that the most appropriate wavelengths are 525 nm for incisors and 590 nm for premolars, as it balanced the light transmission through the tooth structure and the sensitivity for detecting changes in blood concentration. Further research is expected to expand the range of applications of TLP in premolars.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106178"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibiofilm, regenerative and bone homeostasis potential of the synergistic association of synoeca-MP peptide with chlorhexidine in oral cavity opportunistic infections","authors":"Ingrid Aquino Reichert Barin ,&nbsp;Johnny Carvalho da Silva ,&nbsp;Raquel Figuerêdo Ramos ,&nbsp;Stella Maris de Freitas Lima ,&nbsp;Ana Paula de Castro Cantuária ,&nbsp;Poliana Amanda Oliveira Silva ,&nbsp;Elaine Maria Guará Lôbo Dantas ,&nbsp;Danilo César Mota Martins ,&nbsp;Nelson Gomes de Oliveira Júnior ,&nbsp;Osmel Fleitas Martínez ,&nbsp;Jeeser Alves de Almeida ,&nbsp;Marcelo Henrique Soller Ramada ,&nbsp;Octávio Luiz Franco ,&nbsp;Taia Maria Berto Rezende","doi":"10.1016/j.archoralbio.2025.106177","DOIUrl":"10.1016/j.archoralbio.2025.106177","url":null,"abstract":"<div><h3>Objective</h3><div>Synoeca-MP is an antimicrobial peptide that belongs to the class of defense peptides, known for their antimicrobial and immunomodulatory properties. To evaluate <em>in vitro</em> the association between synoeca-MP peptide and chlorhexidine, regarding their antimicrobial and antibiofilm activities, saliva stability, effect on tissue repair, bone resorption processes, and mineralized matrix formation.</div></div><div><h3>Design</h3><div>Initially, the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and antibiofilm concentration were determined. The synergism and degradation of synoeca-MP and chlorhexidine in human saliva were assessed. Furthermore, biocompatibility was evaluated using MTT assays, hemolytic assays, and proliferation and migration assays of periodontal ligament cells. Finally, bone homeostasis was evaluated through osteoclastogenesis assays, alkaline phosphatase determination, and mineralized matrix formation assay with SaOs-2 and ligament cells.</div></div><div><h3>Results</h3><div>The antimicrobial and antibiofilm activity against the tested microorganisms was confirmed. Low synergistic concentrations of the synoeca-MP and chlorhexidine combination inhibited tested microorganisms. The association of these molecules remained stable in healthy saliva. Nevertheless, it degraded as the severity of periodontal disease increased. Additionally, lower synergistic concentrations of the combination were not cytotoxic to human cells, promoted the proliferation and migration of ligament cells, inhibited osteoclastogenesis, and increased mineral matrix formation of ligament cells and SaOs-2.</div></div><div><h3>Conclusions</h3><div>Synoeca-MP and chlorhexidine combination shows potential for oral diseases treatment, as evidenced by its antimicrobial activity, regenerative potential, saliva stability, and bone homeostasis. It may be particularly effective for opportunistic oral infections and in conjunction with mechanical therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106177"},"PeriodicalIF":2.2,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143076670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preventive effects of taxifolin on dental caries in vitro and in vivo","authors":"Ye Wang ,&nbsp;Siyi Zhu ,&nbsp;Yaqi Chi ,&nbsp;Di Fu ,&nbsp;Lin Yao ,&nbsp;Mengzhen Ji ,&nbsp;Li Jiang ,&nbsp;Qi Han ,&nbsp;Ling Zou","doi":"10.1016/j.archoralbio.2025.106174","DOIUrl":"10.1016/j.archoralbio.2025.106174","url":null,"abstract":"<div><h3>Objectives</h3><div>The present study aimed to explore the inhibitory effect of taxifolin (TAX) on <em>Streptococcus mutans (S. mutans) in vitro</em> and evaluated the anti-caries efficacy of TAX <em>in vivo</em>.</div></div><div><h3>Design</h3><div>The anti-microbial and anti-biofilm properties of TAX were examined on the <em>S. mutans</em>, and the results were preliminarily verified by quantitative real-time PCR. Polarized light microscopy and transverse microradiography were used to detect the effect of TAX on inhibiting enamel demineralization. The effect of TAX on the remineralization of demineralized enamel was analyzed by a microhardness tester, atomic force microscope, and transverse microradiography. The rat dental caries model was constructed to explore the anti-caries effect of TAX <em>in vivo</em>.</div></div><div><h3>Results</h3><div>The minimum inhibitory concentration of TAX against <em>S. mutans</em> was 1 mg/mL. The 1 mg/mL TAX impeded the biofilm formation, destroyed the biofilm structure, and effectively prevented enamel demineralization caused by <em>S. mutans</em>. Both the 0.5 mg/mL and 1 mg/mL TAX-treated groups exhibited a higher percentage of surface microhardness recovery, along with lower surface roughness, mineral loss, and lesion depth. Additionally, 1 mg/mL TAX demonstrated the ability to inhibit the initiation and progression of caries in rats, while also proving to be biologically safe.</div></div><div><h3>Conclusions</h3><div>TAX had a significant inhibitory effect on <em>S. mutans</em>, could inhibit enamel demineralization and promote remineralization of demineralized enamel, and showed a promising anti-caries effect <em>in vivo</em>.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106174"},"PeriodicalIF":2.2,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asiatic acid methyl ester, a new asiaticoside derivative, induces osteogenic differentiation of hPDLCs","authors":"Piyamas Sumrejkanchanakij ,&nbsp;Atika Resti Fitri ,&nbsp;Prasit Pavasant ,&nbsp;Suconta Chareonvit ,&nbsp;Aye Chan Khine Lin ,&nbsp;Supakarn Chamni","doi":"10.1016/j.archoralbio.2025.106175","DOIUrl":"10.1016/j.archoralbio.2025.106175","url":null,"abstract":"<div><h3>Objective</h3><div>Asiaticoside has the capacity to induce osteogenic differentiation of human periodontal ligament cells (hPDLCs) through Wnt (Wingless-related integration site) signaling. A modified chemical structure (by removing glycoside side chain), referred to as asiatic acid methyl ester (AA1), has been constructed and evaluated for its capacity to induce osteogenic differentiation.</div></div><div><h3>Design</h3><div>hPDLCs viability was determined by MTT assay. The mRNA expression were analyzed by using quantitative real time PCR. The subcellular localization of β-catenin was demonstrated by immunofluorescence staining. hPDLCs were cultured in osteogenic medium and the deposited calcium was detected by alizarin red staining. The protein expressions were examined by immunofluorescence and western blot.</div></div><div><h3>Results</h3><div>AA1 at a concentration 50 µM or below was not toxic to hPDLCs whereas asiaticoside had revealed no impact on cell viability at any concentration tested. The results showed that 2.5 µM of AA1 and 100 µM of asiaticoside significantly induced gene expression of osterix and dentin matrix protein1 (<em>DMP1</em>), concomitant with a formation of mineralized nodules. AA1 activated Wnt signaling as shown by an increase of <em>WNT3A</em> expression and nuclear translocation of β-catenin. This activation was effectively inhibited when cells were pretreated with recombinant human Dickkopf1 (rhDKK1). rhDKK1 significantly attenuated the AA1-induced expression of DMP1 at both mRNA and protein level.</div></div><div><h3>Conclusions</h3><div>These results confirm a common mechanism between asiaticoside and AA1 in fostering osteogenic differentiation of hPDLCs with a higher potency of AA1. In summary, this study has introduced a novel derivative of asiaticoside, which potentially enhance therapeutic effectiveness for periodontal regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106175"},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The interplay of edentulism, smoking, microbiota, oral rehabilitation on cytokineprofile and different conditions of hypertension","authors":"Pillar Gonçalves Pizziolo ,&nbsp;Lorena Mosconi Clemente ,&nbsp;Aline Barbosa Ribeiro ,&nbsp;Viviane de Cássia Oliveira ,&nbsp;Ana Paula Macedo ,&nbsp;Hélio César Salgado ,&nbsp;Rubens Fazan-Junior ,&nbsp;Evandro Watanabe ,&nbsp;Cláudia Helena Silva-Lovato ,&nbsp;Adriana Barbosa Ribeiro","doi":"10.1016/j.archoralbio.2025.106176","DOIUrl":"10.1016/j.archoralbio.2025.106176","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the influence of edentulism, smoking, microbiota, and oral rehabilitation on the cytokine profile in healthy and hypertensive edentulous individuals using complete dentures.</div></div><div><h3>Design</h3><div>This case-control study was divided into four groups: normotensives (control group - NH), controlled hypertensives (case group 1 - CH), unreported hypertensives (case group 2 - UnrH), and uncontrolled hypertensives (case group 3 - UncH). The participants were characterized by sociodemographic data, clinical and behavioral information, and systolic and diastolic blood pressure. The microbial load of <em>Candida</em> spp., <em>Staphylococcus</em> spp., enterobacteria, and <em>mutans streptococci</em> was evaluated by quantifying colony-forming unit. Salivary flow and salivary cytokines (IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, and IL-17) were quantified in unstimulated saliva by mL per minute and by flow cytometry, respectively.</div></div><div><h3>Results</h3><div>Eighty patients (66 ± 7.2 years) were evaluated. The duration of edentulism was positively associated with systolic blood pressure (p = 0.012). Patients with non-functional denture rehabilitation, those with only upper complete dentures, showed significantly higher systolic blood pressure (p = 0.024) and levels of IL-2 (p = 0.024), predominantly in UncH. The colony-forming unit of <em>mutans streptococci</em> on the denture was higher in UncH and showed a negative association with smoking habit, and this had a positive association with salivary cytokines (IL-4, IL-2, IL-17, IFN-γ) and CVD.</div></div><div><h3>Conclusions</h3><div>The interplay between edentulism, smoking, and oral rehabilitation significantly impacts the cytokine profile, particularly in hypertensive conditions. Smoking habits modulated microbiota and interleukin profile, especially in cardiovascular patients. Moreover, non-functional dentures are associated with uncontrolled hypertension, marked by increased systolic blood pressure and IL-2.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106176"},"PeriodicalIF":2.2,"publicationDate":"2025-01-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Survival of periodontal ligament myofibroblasts after short-term mechanical strain in rats and in vitro: Could myofibroblasts contribute to orthodontic relapse?","authors":"Raquel Veloso Varanda ,&nbsp;Jyoti Kumari ,&nbsp;René E.M. van Rheden ,&nbsp;Vincent M.J.I. Cuijpers ,&nbsp;Marjon Bloemen ,&nbsp;Fleur Göllesch ,&nbsp;Johannes W. Von den Hoff ,&nbsp;Sjoerd Henneman ,&nbsp;Rui Xie ,&nbsp;Frank A.D.T.G. Wagener ,&nbsp;C. Maarten Suttorp","doi":"10.1016/j.archoralbio.2025.106173","DOIUrl":"10.1016/j.archoralbio.2025.106173","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate <em>in vivo</em> whether myofibroblasts formed in the PDL after exposure to short-term high experimental orthodontic forces in rats survive. To study <em>in vitro</em> whether human PDL fibroblasts can differentiate into myofibroblasts and survive when chemical or mechanical stimuli are removed.</div></div><div><h3>Design</h3><div>Nine 6-week-old male Wistar rats were used in this experiment. Rat molars were exposed to high but rapidly decreasing experimental orthodontic forces by applying a rubber band and analyzed for the presence of myofibroblasts using ASMA staining. <em>In vitro</em>, human periodontal ligament (PDL) fibroblasts were exposed to transforming growth factor β1 (TGFβ1) and/or mechanical stress and monitored for myofibroblast formation and survival after these stimuli were abrogated.</div></div><div><h3>Results</h3><div><em>In vivo</em> exposure to orthodontic forces strongly induced myofibroblast formation in the stretched regions of the PDL. Furthermore, many PDL myofibroblasts remained present 6 days after exposure to these short-term high orthodontic forces. Human PDL fibroblasts were shown to differentiate into myofibroblasts after 2 days of TGFβ1 exposure and survive for at least 2 more days after removing chemical stimuli (TGFβ1) or mechanical strain. Under in vitro conditions, both TGFβ1 and mechanical strain for 3 days promoted (myo)fibroblast formation, and these cells persisted for 3 more days after the removal of both stimuli.</div></div><div><h3>Conclusions</h3><div>PDL myofibroblasts survive after the removal of mechanical strain <em>in vivo</em> and <em>in vitro</em>. This supports the hypothesis that myofibroblasts, which form in response to mechanical strain and chemical cues in the periodontal ligament (PDL), play a role in relapse following orthodontic tooth movement.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106173"},"PeriodicalIF":2.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of mandibular overdentures on maxillary resorption and its association with masticatory function: A prospective longitudinal study with 3 years of follow-up","authors":"Fernanda Faot ,&nbsp;Samille Biasi Miranda ,&nbsp;Laura Lourenço Morel ,&nbsp;Anna Paula Da Rosa Possebon ,&nbsp;Alessandra Juliê Schuster ,&nbsp;Luciana de Rezende Pinto ,&nbsp;Altair Antoninha Del Bel Cury","doi":"10.1016/j.archoralbio.2024.106171","DOIUrl":"10.1016/j.archoralbio.2024.106171","url":null,"abstract":"<div><h3>Objective</h3><div>This longitudinal clinical study monitored annually the maxillary and mandibular bone remodeling and masticatory function in complete denture (CD) wearers rehabilitated with implant-retained mandibular overdentures (MO) over three years and combined radiographic and masticatory function data to assess the correlation between bone remodeling and masticatory function.</div></div><div><h3>Design</h3><div>Thirty-nine MO wearers were monitored annually to assess changes in: i) residual ridge in the anterior and posterior maxillary region; ii) posterior height and posterior area index (PAI) in the mandible; and iii) masticatory function. Bone remodeling was measured through linear and angular measurements using panoramic radiographs. Masticatory function was evaluated by swallowing threshold test, to determine the median particle size (X50) and homogenization index (B). A mixed-effects multilevel regression model estimated differences between radiological and masticatory parameters over time, with associations verified using Pearson's correlation test.</div></div><div><h3>Results</h3><div>Significant changes in mandibular bone variables (PAI and L1/R1, L2/R2, L3/R3; p ≤ 0.01) occurred when transitioning from CD to MO, showing a reduction in PAI values and slight reduction in mandibular linear measurements. Improvements in masticatory parameters were also observed, highlighting the benefits of transitioning to MO. Over three years of MO usage, significant changes in all radiographic bone parameters were found, with increased PAI and maxillary bone values in the first year. The correlations between masticatory function and radiological parameters indicated that mandibular morphology can significantly influence masticatory function especially in patients wearing CDs. Following the transition to MO, only one consistent significant correlation was observed between X50 and R3/L3 at the 1-year and 3-year intervals.</div></div><div><h3>Conclusion</h3><div>Continuous changes in bone parameters were observed in MO wearers, with stabilization of maxillary ridge resorption and ongoing mandibular remodeling, alongside slight changes in masticatory function, suggesting transitory correlations between linear changes in the posterior region of mandible and masticatory function over time.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106171"},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of ionizing radiation on osteoblastic cells: In vitro insights into the etiopathogenesis of osteoradionecrosis","authors":"Bruna Bastos Silveira ,&nbsp;Larissa Di Carvalho Melo ,&nbsp;Mylene Martins Monteiro ,&nbsp;Juliana Amorim dos Santos ,&nbsp;Paula Elaine Diniz dos Reis ,&nbsp;Angelica Amorim Amato ,&nbsp;Taia Maria Berto Rezende ,&nbsp;Eliete Neves Silva Guerra","doi":"10.1016/j.archoralbio.2024.106172","DOIUrl":"10.1016/j.archoralbio.2024.106172","url":null,"abstract":"<div><h3>Objective</h3><div>This <em>in vitro</em> study aimed to analyze the effects of ionizing radiation on immortalized human osteoblast-like cells (SaOS-2) and further assess their cellular response in co-culture with fibroblasts. These analyses, conducted in both monoculture and co-culture, are based on two theoretical models of osteoradionecrosis – the theory of hypoxia and cellular necrosis and the theory of the radiation-induced fibroatrophic process.</div></div><div><h3>Design</h3><div>SaOS-2 cells were exposed to ionizing radiation and evaluated for cell viability, nitric oxide (NO) production, cellular morphology, wound healing, and gene expression related to the PI3K-AKT-mTOR pathway. SaOS-2 cells were co-cultured with human gingival fibroblasts using transwell membranes and subjected to the same irradiation. Subsequent evaluations included cell viability, NO levels, and gene expression analysis.</div></div><div><h3>Results</h3><div>After 24 hours, a 16 Grays dose reduced cell viability by 40 % (p &lt; 0.0001) and increased NO production by 14 % (p &lt; 0.05). Additionally, the nuclear area was enlarged by 18 % (p &lt; 0.01), and the nucleus-to-cytoplasm ratio in non-stimulated cells was around 33 %, but after radiation, this ratio increased to nearly 100 %. Also, there was a delay in wound closure of 6.6 % (p &lt; 0.0001) post-irradiation and a trend toward down-regulation of genes related to the PI3K–AKT–mTOR pathway (p &gt; 0.05). Under co-culture conditions, the dose of 16 Grays did not affect cell viability but increased NO production by 14 % (p &lt; 0.001) and tended to up-regulate markers of the PI3K–AKT–mTOR pathway (p &gt; 0.05).</div></div><div><h3>Conclusions</h3><div>The findings of this study demonstrate that an irradiation dose of 16 Grays induces a reduction in cell viability, an increase in NO production, and various other metabolic and morphologic effects on osteoblastic cells while emphasizing the impact of intercellular interaction in the etiopathogenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106172"},"PeriodicalIF":2.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}