Archives of oral biology最新文献_第6页

PRELP inhibits the progression of oral squamous cell carcinoma via inactivation of the NF-κB pathway PRELP 通过抑制 NF-κB 通路抑制口腔鳞状细胞癌的发展

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-08-13 DOI: 10.1016/j.archoralbio.2024.106068

Xiaoni Sun, Luyi Chai, Bingjie Wang, Jianbo Zhou

{"title":"PRELP inhibits the progression of oral squamous cell carcinoma via inactivation of the NF-κB pathway","authors":"Xiaoni Sun, Luyi Chai, Bingjie Wang, Jianbo Zhou","doi":"10.1016/j.archoralbio.2024.106068","DOIUrl":"10.1016/j.archoralbio.2024.106068","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to investigate the role and molecular mechanism of proline/arginine-rich end leucine-rich repeat protein (PRELP), a secreted protein in extracellular matrix, in oral squamous cell carcinoma (OSCC) progression.</p></div><div><h3>Design</h3><p>PRELP expression in OSCC was analyzed in the Gene Set Enrichment (GSE) 138206, GSE37991, and GSE23558 datasets as well as cell lines. Also, PRELP expression and its relationship with prognosis and immune infiltration in head and neck squamous cell carcinoma (HNSCC) were confirmed by bioinformatics analysis. The proliferation, apoptosis, invasion, epithelial-to-mesenchymal transition (EMT) and NF-κB activation were detected after alteration of PRELP expression in OSCC cells using CCK-8, EdU, flow cytometry, Transwell, real-time PCR, immunofluorescence and Western blot. Additionally, an NF-κB inhibitor PDTC was used to confirm the regulation mechanism of PRELP.</p></div><div><h3>Results</h3><p>The expression of PRELP in OSCC tissues, cells and in HNSCC samples was low. HNSCC patients with higher PRELP expression was associated with longer overall survival. A positive correlation between PRELP expression and immune cell infiltration was found in HNSCC. Upregulation of PRELP inhibited, whereas PRELP silencing promoted, the proliferation, invasion and EMT of OSCC cells. Also, overexpression of PRELP promoted cell apoptosis. Mechanistically, PRELP suppressed p65 phosphorylation and nuclear translocation. And PDTC treatment partially reversed the influences of PRELP knockdown on the malignant behaviors in OSCC cells.</p></div><div><h3>Conclusion</h3><p>PRELP suppressed OSCC progression via inactivation of the NF-κB pathway. Targeting PRELP may be a potential approach for OSCC treatment.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106068"},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141991253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oral microbiome insights: Tracing acidic culprits in dental caries with functional metagenomics 洞察口腔微生物组：利用功能元基因组追踪龋齿的酸性元凶

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-08-13 DOI: 10.1016/j.archoralbio.2024.106064

Annapurna Gupta , A. Shivachandran , Lilly M. Saleena

{"title":"Oral microbiome insights: Tracing acidic culprits in dental caries with functional metagenomics","authors":"Annapurna Gupta , A. Shivachandran , Lilly M. Saleena","doi":"10.1016/j.archoralbio.2024.106064","DOIUrl":"10.1016/j.archoralbio.2024.106064","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to investigate the presence and abundance of acid-producing bacteria in dental caries samples using functional gene prediction techniques.</p></div><div><h3>Design</h3><p>A total of 24 dental caries samples were collected for analysis. DNA isolation was performed followed by shotgun metagenomic sequencing. Functional gene prediction techniques were used to identify enzymes responsible for acid production from primary metabolites. Enzymes responsible for converting primary metabolites into acids were identified from the KEGG database. Subsequently, 840 contigs were examined, and their genus and species were characterized.</p></div><div><h3>Results</h3><p>Analysis of the obtained data revealed 31 KEGG IDs corresponding to enzymes involved in the conversion of primary metabolites into acids. All 117 identified genera from the contig analysis were found to be part of the oral microbiome. In addition, A higher prevalence of acid-producing bacteria was noted in dental caries samples compared to earlier reports.</p></div><div><h3>Conclusion</h3><p>The study indicates the significant role of acid-producing bacteria in the initiation and progression of dental caries. The findings highlight the importance of microbial activity in the demineralization process of tooth enamel. Methods for preventing dental decay may be promising if specific measures are implemented to reduce the amount of acid produced by oral bacteria.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106064"},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142096645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Testing the patterning cascade model of cusp development in Macaca fascicularis mandibular molars 测试猕猴下颌臼齿尖突发育的模式级联模型

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-08-08 DOI: 10.1016/j.archoralbio.2024.106067

Simon A. Chapple , Tanya M. Smith , Matthew M. Skinner

{"title":"Testing the patterning cascade model of cusp development in Macaca fascicularis mandibular molars","authors":"Simon A. Chapple , Tanya M. Smith , Matthew M. Skinner","doi":"10.1016/j.archoralbio.2024.106067","DOIUrl":"10.1016/j.archoralbio.2024.106067","url":null,"abstract":"<div><h3>Objective</h3><p>Molar crown configuration plays an important role in systematics, and functional and comparative morphology. In particular, the number of cusps on primate molars is often used to identify fossil species and infer their phylogenetic relationships. However, this variability deserves renewed consideration as a number of studies now highlight important developmental mechanisms that may be responsible for the presence of molar cusps in some mammalian taxa. Experimental studies of rodent molars suggest that cusps form under a morphodynamic, patterning cascade model of development (PCM) that involve the iterative formation of enamel knots. This model posits that the size, shape and location of the first-forming cusps determines the presence and positioning of later-forming cusps.</p></div><div><h3>Design</h3><p>Here we test whether variation in accessory cusp presence in 13 <em>Macaca fascicularis</em> mandibular second molars (M2s) is consistent with predictions of the PCM. Using micro-CT, we imaged these M2s and employed geometric morphometrics to examine whether shape variation in the enamel-dentine junction (EDJ) correlates with accessory cusp presence.</p></div><div><h3>Results</h3><p>We find that accessory cusp patterning in macaque M2s is broadly consistent with the PCM. Molars with accessory cusps were larger in size and possessed shorter relative cusp heights compared to molars without accessory cusps. Peripheral cusp formation was also associated with more centrally positioned primary cusps, as predicted by the PCM.</p></div><div><h3>Conclusions</h3><p>While these results demonstrate that a patterning cascade model is broadly appropriate for interpreting cusp variation in <em>Macaca fascicularis</em> molars<em>,</em> it does not explain all manifestations of accessory cusp expression in this sample.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106067"},"PeriodicalIF":2.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001882/pdfft?md5=a0e62a0516c8fac801db9be99e5b8f65&pid=1-s2.0-S0003996924001882-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of simvastatin on osteoblast functionality in the presence of titanium dioxide particles In-vitro 辛伐他汀在二氧化钛颗粒存在下对成骨细胞功能的影响体外试验

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-08-06 DOI: 10.1016/j.archoralbio.2024.106065

Ahmad Fawaz , Marwan Mansoor Mohammed , Asmaa Ismail , K.G.Aghila Rani , A.R. Samsudin

{"title":"The influence of simvastatin on osteoblast functionality in the presence of titanium dioxide particles In-vitro","authors":"Ahmad Fawaz , Marwan Mansoor Mohammed , Asmaa Ismail , K.G.Aghila Rani , A.R. Samsudin","doi":"10.1016/j.archoralbio.2024.106065","DOIUrl":"10.1016/j.archoralbio.2024.106065","url":null,"abstract":"<div><h3>Objective</h3><p>Leaching of particles from dental titanium implant surfaces into preimplant microenvironment causes detrimental effects on bone cells. The current study investigated influence of simvastatin in mitigating adverse pro-inflammatory effects of titanium dioxide (TiO<sub>2</sub>) micro (MP) and nano (NP) particles on hFOB 1.19 cells <em>in vitro.</em></p></div><div><h3>Design</h3><p>Viability of hFOB 1.19 cells following exposure to varying concentrations of TiO<sub>2</sub> MPs and NPs and simvastatin were measured by XTT assay. hFOB 1.19 cells were treated with 100 µg/mL of TiO<sub>2</sub> MPs, 100 µg/mL of TiO<sub>2</sub> NPs, 0.1 µM simvastatin, 100 µg/mL of TiO<sub>2</sub> MPs+ 0.1 µM simvastatin and 100 µg/mL of TiO<sub>2</sub> NPs+ 0.1 µM simvastatin. After 24 h, ROS was measured by flow cytometry. On day 14, real-time PCR analysis for pro-inflammatory cytokines and bone formation markers was done for TNFα, IL1β, osteocalcin, ALP, and Col1 markers; while ALP and RANKL/OPG ratio were determined by colorimetric and ELISA assays respectively. Further, mineralization study using Alizarin Red S staining (ARS) and calcium quantification were performed.</p></div><div><h3>Results</h3><p>Exposure of hFOB to TiO<sub>2</sub> MPs and NPs generated ROS and reduced cell viability significantly, with upregulation of pro-inflammatory markers TNFα and IL1β and downregulation of bone formation markers OC and increased RANKL/OPG ratio and lowered degree of mineralization. Treatment with 0.1 µM of simvastatin treatment reversed the effects by mitigating oxidative stress, dampening pro-inflammatory markers, upregulation of bone formation markers, lowering RANKL/OPG ratio and increasing degree of mineralization.</p></div><div><h3>Conclusion</h3><p>Simvastatin possesses antioxidant, anti-inflammatory, and pro-osteogenic properties that may support bone healing around titanium implants.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106065"},"PeriodicalIF":2.2,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141985780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis LncRNA MIAT 在牙周炎中的诊断意义以及通过 miR-204-5p/DKK1 轴影响牙周韧带成纤维细胞的分子机制

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-08-04 DOI: 10.1016/j.archoralbio.2024.106066

Yu Ren , Jiwen Zheng , Yang Cao , Yu Zhu , Zhuo Ling , Zhiqiang Zhang , Mingke Huang

{"title":"Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis","authors":"Yu Ren , Jiwen Zheng , Yang Cao , Yu Zhu , Zhuo Ling , Zhiqiang Zhang , Mingke Huang","doi":"10.1016/j.archoralbio.2024.106066","DOIUrl":"10.1016/j.archoralbio.2024.106066","url":null,"abstract":"<div><h3>Objective</h3><p>This study investigated the clinical importance of long noncoding RNA myocardial infarction-associated transcript (MIAT) in periodontitis and its impact on the functional regulation of human periodontal ligament fibroblasts (hPDLFs).</p></div><div><h3>Methods</h3><p>Ninety-eight periodontitis patients and 74 healthy controls were enrolled. In vitro cellular models were created using <em>Porphyromonas gingivalis</em> lipopolysaccharide (Pg-LPS) to stimulate hPDLFs. Real-time quantitative polymerase chain reaction was used to measure mRNA levels of MIAT and osteogenic factors. Inflammation factor concentration was assessed using an enzyme-linked immunosorbent assay. Cell viability and apoptosis were examined by cell counting kit −8 and flow cytometry assay. The targeting relationship was verified by the dual-luciferase reporter and RNA Immunoprecipitation assay.</p></div><div><h3>Results</h3><p>Highly expressed MIAT and Dicckopf-1 (DDK1), and lowly expressed miR-204–5p were found in the gingival crevicular fluid of periodontitis patients and Pg-LPS induced hPDLFs. MIAT has a sensitivity of 76.53 % and a specificity of 86.49 % for identifying patients with periodontitis among healthy individuals. MIAT acts as a sponge for miR-204–5p and upregulates DDK1 mRNA expression. Silencing of MIAT diminished the promotion of apoptosis and inflammation in hPDLFs by Pg-LPS and enhanced osteogenic differentiation. However, a miR-204–5p inhibitor significantly reversed the effect of silenced MIAT.</p></div><div><h3>Conclusions</h3><p>MIAT may act as a promising biomarker for periodontitis. It modulates apoptosis, inflammation, and osteogenic differentiation of PDLFs by focusing on the miR-204–5p/DKK1 axis, indicating its potential as a new therapeutic target for treating periodontitis.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106066"},"PeriodicalIF":2.2,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria 绿茶中富含的儿茶素表没食子儿茶素-3-棓酸盐对牙周病相关细菌的抗菌作用。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-07-31 DOI: 10.1016/j.archoralbio.2024.106063

Mayu Higuchi, Yuki Abiko, Jumpei Washio, Nobuhiro Takahashi

{"title":"Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria","authors":"Mayu Higuchi, Yuki Abiko, Jumpei Washio, Nobuhiro Takahashi","doi":"10.1016/j.archoralbio.2024.106063","DOIUrl":"10.1016/j.archoralbio.2024.106063","url":null,"abstract":"<div><h3>Objective</h3><p>Epigallocatechin-3-gallate (EGCG), a catechin abundant in green tea, exhibits antibacterial activity. In this study, the antimicrobial effects of EGCG on periodontal disease-associated bacteria (<em>Porphyromonas gingivalis</em>, <em>Prevotella intermedia</em>, <em>Prevotella nigrescens</em>, <em>Fusobacterium nucleatum</em>, and <em>Fusobacterium periodontium</em>) were evaluated and compared with its effects on <em>Streptococcus mutans</em>, a caries-associated bacterium.</p></div><div><h3>Results</h3><p>Treatment with 2 mg/ml EGCG for 4 h killed all periodontal disease-associated bacteria, whereas it only reduced the viable count of <em>S. mutans</em> by about 40 %. Regarding growth, the periodontal disease-associated bacteria were more susceptible to EGCG than <em>S. mutans</em>, based on the growth inhibition ring test<em>.</em> As for metabolism, the 50 % inhibitory concentration (IC<sub>50</sub>) of EGCG for bacterial metabolic activity was lower for periodontal disease-associated bacteria (0.32–0.65 mg/ml) than for <em>S. mutans</em> (1.14 mg/ml). Furthermore, these IC<sub>50</sub> values were negatively correlated with the growth inhibition ring (r = −0.73 to −0.86). EGCG induced bacterial aggregation at the following concentrations: <em>P. gingivalis</em> (>0.125 mg/ml), <em>F. periodonticum</em> (>0.5 mg/ml), <em>F. nucleatum</em> (>1 mg/ml), and <em>P. nigrescens</em> (>2 mg/ml). <em>S. mutans</em> aggregated at an EGCG concentration of > 1 mg/ml.</p></div><div><h3>Conclusion</h3><p>EGCG may help to prevent periodontal disease by killing bacteria, inhibiting bacterial growth by suppressing bacterial metabolic activity, and removing bacteria through aggregation.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106063"},"PeriodicalIF":2.2,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001845/pdfft?md5=ad101c84b7d1199d7d35809514c558ee&pid=1-s2.0-S0003996924001845-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A persistent mineralization process in alveolar bone throughout the postnatal growth stage in rats 大鼠牙槽骨在整个出生后生长阶段的持续矿化过程。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-07-30 DOI: 10.1016/j.archoralbio.2024.106062

Xinli Lv, Jixiao Wang, Fulan Wei

{"title":"A persistent mineralization process in alveolar bone throughout the postnatal growth stage in rats","authors":"Xinli Lv, Jixiao Wang, Fulan Wei","doi":"10.1016/j.archoralbio.2024.106062","DOIUrl":"10.1016/j.archoralbio.2024.106062","url":null,"abstract":"<div><h3>Objective</h3><p>Alveolar bone quality is essential for the maxillofacial integrity and function, and depends on alveolar bone mineralization. This study aims to investigate the in vivo changes in alveolar bone mineralization, from the perspective of mineral deposition and crystal transition in postnatal rats.</p></div><div><h3>Design</h3><p>Nine postnatal time points of Wistar rats, ranging from day 1 to 56, were set to obtain the maxillary alveolar bone samples. Each time point consisted of ninety rats, with 45 females and 45 males. Macromorphology of alveolar bone was reconducted by Micro-Computed Tomography and the mineral content was quantified via Thermogravimetric analysis, Scanning Electron Microscope, High-Resolution Transmission Electron Microscopy and vibrational spectroscopy. Furthermore, the crystallinity and composition were characterized by vibrational spectroscopy, X-ray Diffraction, X-ray Photoelectron Spectroscopy and Selected Area Electron Diffraction.</p></div><div><h3>Results</h3><p>The progressive increase of mineral deposition was accompanied by substantial growth in alveolar bone mass and volume in postnatal rats. Whereas the mineral percentage initially decreased and then increased, reaching a nadir on postnatal day 14 (P14) when tooth eruption was first observed. Besides, localized mineralization was initiated by the formation of amorphous precursors and then converted into mineral crystals, while there was no statistically significant change in the average crystallinity of the bone during growth.</p></div><div><h3>Conclusion</h3><p>Mineralization of alveolar bone is ongoing throughout the early growth in postnatal rats. Mineral deposition increases with age, whereas the crystallinity remains stable within a certain range. Besides, the mineral percentage reaches its lowest point on P14, which may be attributed to tooth eruption.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106062"},"PeriodicalIF":2.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Neohesperidin exerts subtle yet comprehensive regulation of mouse dental papilla cell-23 in vitro 新橙皮甙对体外小鼠牙乳头细胞-23具有微妙而全面的调节作用。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-07-24 DOI: 10.1016/j.archoralbio.2024.106055

Sheng Zhang , Jian Guan , Jing Lv , Xinhe Dong , Runhang Li , Yuhong Wang , Xing-ai Jin

{"title":"Neohesperidin exerts subtle yet comprehensive regulation of mouse dental papilla cell-23 in vitro","authors":"Sheng Zhang , Jian Guan , Jing Lv , Xinhe Dong , Runhang Li , Yuhong Wang , Xing-ai Jin","doi":"10.1016/j.archoralbio.2024.106055","DOIUrl":"10.1016/j.archoralbio.2024.106055","url":null,"abstract":"<div><h3>Objective</h3><p>The molecular regulation of odontoblasts in dentin formation remains largely uncharacterized. Using neohesperidin (NEO), a well-documented osteoblast regulator, we investigated whether and how NEO participates in odontoblast regulation through longitudinal treatments using various doses of NEO.</p></div><div><h3>Design</h3><p>Mouse dental papilla cell-23 (MDPC-23) served as a model for odontoblasts. MDPC-23 were treated with various doses of NEO (0, 1, 5, 10, 15, 20 μmol/L). Proliferation was assessed using the Cell counting kit-8 assay. Survival/apoptosis was assayed by live/dead ratio. Migration capability was assessed using scratch healing and Transwell migration assays. Mineralization was assessed using alkaline phosphatase staining and alizarin red staining. The expression levels of four key genes (Runx2, osteocalcin [OCN], β-catenin, and bone morphogenetic protein [BMP]−2) representing NEO-induced differentiation of MDPC-23 were measured by quantitative reverse transcription polymerase chain reaction.</p></div><div><h3>Results</h3><p>The proliferation trajectories of MDPC-23 treated with the five doses of NEO demonstrated similar curves, with a rapid increase in the 10 μmol/L NEO condition after 48 h of treatment. Similar dose-dependent trajectories were observed for survival/apoptosis. All four key genes representing odontogenic differentiation were upregulated in MDPC-23 induced by NEO treatments at two optimal doses (5 μmol/L and 10 μmol/L). Optimal migration and mobility trajectories were observed in MDPC-23 treated with 10 μmol/L NEO. Optimal mineralization was observed in MDPC-23 treated with 5 μmol/L NEO.</p></div><div><h3>Conclusion</h3><p>NEO can subtly regulate odontoblast proliferation, differentiation, migration, and mineralization in vitro. NEO at 5–10 μmol/L offers a safe and effective perspective for clinical promotion of dentin bridge formation in teenagers.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106055"},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arecoline promotes fibroblast activation and M2-macrophage polarization by up-regulating the expression of IL-4 阿雷科林通过上调 IL-4 的表达，促进成纤维细胞活化和 M2-巨噬细胞极化。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-07-20 DOI: 10.1016/j.archoralbio.2024.106052

Zhenying Yuan , Ming Li

{"title":"Arecoline promotes fibroblast activation and M2-macrophage polarization by up-regulating the expression of IL-4","authors":"Zhenying Yuan , Ming Li","doi":"10.1016/j.archoralbio.2024.106052","DOIUrl":"10.1016/j.archoralbio.2024.106052","url":null,"abstract":"<div><h3>Objective</h3><p>To determine the biological effects of arecoline on oral submucous fibrosis (OSF).</p></div><div><h3>Design</h3><p>The differential genes between OSF tissue and normal oral tissue were collected form GSE64216 dataset, analyzed by Gene Expression Omnibus (GEO) database. Real-time PCR and immunohistochemistry were used to analyze the expression of IL-4 gene and protein in oral tissue. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression of exocrine IL-4 protein in human oral fibroblasts (HOF) pre-treated by arecoline. Cell Counting Kit-8 (CCK-8) and transwell assays were used to analyze the proliferation and migration of HOF cells, respectively. After IL-4 was knocked down by short hairpin (sh) plasmid, the proliferation and migration of HOF cells were detected. Flow cytometry was used to analyze the proportion of M2-macrophages. Real-time PCR and immunohistochemistry were used to verify the expression of biomarker proteins of macrophages in OSF tissues.</p></div><div><h3>Results</h3><p>The expression of IL-4 gene and protein were both up-regulated in OSF tissue. Arecoline could enhance the expression of IL-4 gene and exocrine protein in HOF cells, and promote the proliferation and migration of HOF cells. While knockdown of IL-4 could inhibit arecoline-induced proliferation and migration in HOF cells. The results of flow cytometry showed that recombinant human IL-4 (rhIL-4) protein could increase the proportion of M2-macrophages. Similarly, the results of real-time PCR and immunohistochemistry showed the expression of ARG1 (Biomarker proteins of M2-macrophage) was up-regulated in OSF tissues.</p></div><div><h3>Conclusion</h3><p>Arecoline promotes activation of fibroblasts and polarization of M2-macrophages by up-regulating the expression of IL-4.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106052"},"PeriodicalIF":2.2,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141763188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel missense variant in CAT gene causing acatalasemia with gangrenous periodontitis (Takahara’s disease) 导致伴有坏疽性牙周炎（高原病）的苊血症的 CAT 基因新型错义变异体

IF 2.2 4区医学

Archives of oral biology Pub Date : 2024-07-17 DOI: 10.1016/j.archoralbio.2024.106054

Nehal F. Hassib , Mennat Mehrez , Maha R. Abouzaid , Mostafa I. Mostafa , Rasha M. Elhossini , Mohamed S. Abdel-Hamid

{"title":"A novel missense variant in CAT gene causing acatalasemia with gangrenous periodontitis (Takahara’s disease)","authors":"Nehal F. Hassib , Mennat Mehrez , Maha R. Abouzaid , Mostafa I. Mostafa , Rasha M. Elhossini , Mohamed S. Abdel-Hamid","doi":"10.1016/j.archoralbio.2024.106054","DOIUrl":"10.1016/j.archoralbio.2024.106054","url":null,"abstract":"<div><h3>Objectives</h3><p>Acatalasemia is a very rare disorder characterized by gangrenous oral ulcerations and is caused by biallelic variants in the <em>CAT</em> gene which encodes the catalase enzyme that decomposes the hydrogen peroxide molecules to remove their toxic effect. We report two siblings from a consanguineous Egyptian family presenting with joint hyperlaxity, loose dentitions with gangrenous periodontitis, and early loss of teeth.</p></div><div><h3>Study design</h3><p>The patients were clinically suspected to have the periodontal type of Ehlers-Danlos syndrome and thus genetic testing of <em>C1S</em> and <em>C1R</em> causative genes was carried out first by Sanger sequencing then exome sequencing (ES) was considered.</p></div><div><h3>Results</h3><p>No pathogenic variants were detected in <em>C1S</em> and <em>C1R</em> genes then ES revealed a new homozygous missense variant in the <em>CAT</em> gene segregating in the family, c .635 T > G (p.Met212Arg).</p></div><div><h3>Conclusion</h3><p>We describe the first Egyptian cases with acatalasemia and expand the mutational spectrum of this rare disorder. Premature loss of teeth is an emerging finding in our cases and addresses the hazardous systemic manifestations associated with the disorder. The rarity of inherited orodental diseases renders the accurate diagnosis difficult and complicates the symptoms. Therefore, the use of advanced molecular technologies is highly advisable for early diagnosis and management of patients.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106054"},"PeriodicalIF":2.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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