Archives of oral biology最新文献

{"title":"Retraction notice to “Berberine reduces inflammation of human dental pulp fibroblast via miR-21/KBTBD7 axis” [Archives of Oral Biology 110 (2020) 104630]","authors":"Song Jia , Wu Qishan , Jiang Jin , Sun Degang , Wang Fang , Xin Bingchang , Cui Qi","doi":"10.1016/j.archoralbio.2025.106464","DOIUrl":"10.1016/j.archoralbio.2025.106464","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106464"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145679395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of phenotype switched Candida auris mono-culture and co-culture biofilms on the morphology, viability, and adhesion of hTERT TIGKs and ORL-48 cell lines","authors":"Mukarramah Zainal ,&nbsp;Nurul ‘Izzah Mohd Sarmin ,&nbsp;Mohammad Johari Ibrahim ,&nbsp;Nicola Cirillo ,&nbsp;Stuart G. Dashper ,&nbsp;Mohd Hafiz Arzmi","doi":"10.1016/j.archoralbio.2025.106478","DOIUrl":"10.1016/j.archoralbio.2025.106478","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to determine the paracrine effects of <em>Candida auris</em> phenotypic switching in mono- and co-culture with <em>Staphylococcus aureus</em> on oral epithelial homeostasis and oncogenic progression in phenotypically normal (hTERT TIGKs) and malignant (ORL-48) oral keratinocytes.</div></div><div><h3>Design</h3><div><em>C. auris</em> switched phenotype was scored using Phloxine B, and mono- and co-culture biofilms with <em>S. aureus</em> were developed. hTERT TIGKs and ORL-48 cell lines were independently seeded into 6-well and 96-well plates for dispase and viability test, respectively. The oral cell lines were exposed to phenotypically switched <em>C. auris</em> mono- and co-culture biofilm test cell growth medium (TCGM) for 24 h. Outcomes included cell morphology, metabolic activity/viability (CCK-8), and cell–cell adhesion (dispase assay).</div></div><div><h3>Results</h3><div>Microscopic observation revealed that the biofilm induced damage and disrupted epithelial cell integrity in a paracrine manner. The mono- and co-culture TCGM suppressed the growth of normal cells while promoting the metabolic activity of cancer cells. The adhesion analysis of hTERT TIGKs indicated a strong intercellular cohesion, while ORL-48 cells downregulated intercellular adhesion and compromised cell-cell cohesion.</div></div><div><h3>Conclusion</h3><div><em>C. auris</em> biofilms promote the development of a malignant phenotype by regulating cell viability, promoting epithelial-mesenchymal transition, and adhesion in a switched generation-dependent manner.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106478"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145783979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of stress on pain sensitization and emotional responses in a rat model of persistent TMJ inflammation","authors":"Jardel Francisco Mazzi-Chaves ,&nbsp;Ana Paula Ribeiro Novaes ,&nbsp;Glauce Crivelaro Nascimento ,&nbsp;Christie Ramos Andrade Leite-Panissi","doi":"10.1016/j.archoralbio.2025.106463","DOIUrl":"10.1016/j.archoralbio.2025.106463","url":null,"abstract":"<div><h3>Objective</h3><div>Temporomandibular disorders (TMDs) are frequently associated with psychological distress, including anxiety and depression, which can modulate pain perception and sensitivity. This study investigated the impact of acute and chronic stress on the progression of temporomandibular joint (TMJ) pain, secondary hyperalgesia, and stress-induced affective disturbances.</div></div><div><h3>Design</h3><div>Using a well-established preclinical model, Wistar-Hannover rats underwent persistent TMJ inflammation via intra-articular administration of Freund’s complete adjuvant (CFA). Orofacial mechanical allodynia and secondary hyperalgesia were assessed using von Frey test in the orofacial region and hot plate test in the hind paw. To evaluate the influence of stress on affective behaviors, Acute stress (AS), chronic restraint stress (CRS) and Unpredictable chronic stress (UCS) paradigms were implemented, followed by behavioral assessments using the elevated plus maze (EPM), open field (OF), and sucrose preference tests.</div></div><div><h3>Results</h3><div>Results demonstrated that chronic stress exacerbated CFA-induced orofacial allodynia. TMJ inflammation induced secondary hyperalgesia, with AS partially restoring baseline nociception, while UCS amplified central sensitization. Notably, CRS did not influence hind paw nociception in CFA-injected rats. Behavioral analyses revealed that CFA injection heightened anxiety-like behavior by decreased open-arm exploration. Acute stress further intensified anxiety and impaired exploratory activity, whereas chronic stresses significantly worsened both anxiety- and depression-like behaviors.</div></div><div><h3>Conclusion</h3><div>These findings underscore the complex interplay between stress and pain processing in TMDs, highlighting the detrimental role of chronic stress in exacerbating pain sensitivity and emotional dysregulation. Understanding these mechanisms could lead to more effective, targeted treatments, improving patient outcomes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106463"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BBI608 induces apoptosis in mucoepidermoid carcinoma cells by targeting a post-transcriptional regulatory mechanisms of myeloid cell leukemia-1","authors":"Da-In Choi ,&nbsp;Hyun-Ji Kim ,&nbsp;Dong-Guk Park ,&nbsp;Jae-Han Lee ,&nbsp;Thantrira Porntaveetus ,&nbsp;Sung-Dae Cho","doi":"10.1016/j.archoralbio.2025.106467","DOIUrl":"10.1016/j.archoralbio.2025.106467","url":null,"abstract":"<div><h3>Objectives</h3><div>BBI608 has demonstrated antitumor activity in several human cancers. However, its efficacy against mucoepidermoid carcinoma (MEC) remains unexplored. This study investigated the antitumor potential of BBI608 in MEC cell lines.</div></div><div><h3>Design</h3><div>The antitumor activity of BBI608 in MC3 and YD-15 mucoepidermoid carcinoma (MEC) cell lines was assessed using trypan blue exclusion, Live/Dead, and sphere formation assays. Apoptotic effects were investigated via western blotting, 4′,6-diamidino-2-phenylindole (DAPI) staining, Annexin V-fluorescein isothiocyanate/propidium iodide (V-FITC/PI) double staining, and reverse transcription-quantitative PCR.</div></div><div><h3>Results</h3><div>BBI608 exhibited growth-inhibitory effects in MEC cell lines, decreasing cell viability and sphere formation capacity while increasing cell death. BBI608-induced apoptosis was confirmed by increased cleaved caspase-3 and PARP expression, nuclear morphological changes characteristic of apoptosis, and increased Annexin V positivity. Furthermore, BBI608 significantly downregulated Mcl-1 expression, which contributed to apoptosis induction in MEC cells. This Mcl-1 downregulation appeared to be mediated by both proteasome-dependent protein degradation and translational regulatory mechanisms in MC3 and YD-15 cells, respectively.</div></div><div><h3>Conclusion</h3><div>These findings demonstrate that BBI608 effectively inhibits MEC cell proliferation in vitro by inducing Mcl-1-dependent apoptosis. This suggests BBI608 warrants further investigation as a potential therapeutic agent for MEC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106467"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo immunological evaluation of indomethacin and omega-3 nanocapsules for the treatment of rheumatoid arthritis in the temporomandibular joints of rats","authors":"Victor Augusto Benedicto dos Santos ,&nbsp;Francisco Carlos Groppo ,&nbsp;Thomas Barbin ,&nbsp;Adilson Sartoratto ,&nbsp;Luiz Eduardo Nunes Ferreira ,&nbsp;Michael Henrique Araujo Monteiro ,&nbsp;Sidney Raimundo Figueroba","doi":"10.1016/j.archoralbio.2025.106456","DOIUrl":"10.1016/j.archoralbio.2025.106456","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to develop and characterize indomethacin nanocapsules with an omega-3 oily core and evaluate their potential for treating rheumatoid arthritis in rats, comparing their effects to free-form administration on cytokines IL-1β, IL-10, IL-6, and TNF-α in temporomandibular joint.</div></div><div><h3>Design</h3><div>Nanocapsules were synthesized with omega-3 as the oil phase containing indomethacin. They were characterized for mean hydrodynamic diameter, polydispersity index, zeta potential, morphology (TEM), encapsulation efficiency (HPLC), and cytotoxicity in RAW 264.7 macrophages (MTT assay). For in vivo evaluation, forty-eight adult male Wistar rats (n = 6) underwent rheumatoid arthritis induction via intradermal injection of Complete Freund's Adjuvant (CFA) and bovine type II collagen (CII) at the base of the tail. Rats were treated for 7 days by oral gavage with either nanocapsules (NC5, NC2.5), free indomethacin (IND5, IND2.5), or indomethacin combined with omega-3 (IND5 +ω3, IND2.5 +ω3). Cytokine levels in the temporomandibular joint were subsequently assessed.</div></div><div><h3>Results</h3><div>Nanocapsules displayed a spherical, well-defined morphology with diameters &lt; 250 nm and exhibited lower cytotoxicity in RAW 264.7 cells compared to free-form treatments. In vivo, all treated groups showed significant reductions in IL-1β, IL-6, and TNF-α compared to the CFA group, along with a significant increase in IL-10.</div></div><div><h3>Conclusions</h3><div>Indomethacin nanocapsules with omega-3 effectively reduced pro-inflammatory cytokines and increased anti-inflammatory IL-10, demonstrating a superior immunomodulatory profile compared to free-form treatments.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106456"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Submandibular saliva from male rats modulates osteogenic differentiation of bone marrow progenitor cells In Vitro","authors":"Gastón R. Troncoso ,&nbsp;María V. Gangoiti ,&nbsp;Javier Fernández-Solari ,&nbsp;Claudia E. Mohn ,&nbsp;María S. Molinuevo","doi":"10.1016/j.archoralbio.2025.106484","DOIUrl":"10.1016/j.archoralbio.2025.106484","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of submandibular saliva from male rats on the proliferation and osteoblastic differentiation of bone marrow progenitor cells.</div></div><div><h3>Design</h3><div>Saliva was collected and pooled from adult male rats, and the total protein content was measured. Bone marrow progenitor cells (BMPC) from adult male rats were incubated with varying concentrations of salivary protein (ranging from 0 to 30 µg/mL) to assess cell proliferation. Osteoblastic differentiation was evaluated by measuring alkaline phosphatase activity, collagen type 1 production, mineral nodule formation, and molecular markers of differentiation after 7, 15, or 21 days of saliva exposure in a differentiating culture medium. The pro-inflammatory effects of saliva on BMPC were assessed by measuring tumor necrosis factor-alpha, interleukin 1 beta, prostaglandin E2, and matrix metalloproteinases 2 and 9.</div></div><div><h3>Results</h3><div>Low saliva concentration stimulated BMPC, promoting a pro-secretory and proliferative state. In contrast, higher concentration inhibited both processes under basal conditions. Furthermore, in osteogenic medium, saliva decreased alkaline phosphatase activity, collagen-1 production, and matrix mineralization in BMPC, demonstrating a dose- and time-dependent effect. Saliva also increased the secretion of interleukin 1β, tumor necrosis factor α, prostaglandin E2, and metalloproteinase activity in these cells, which inhibited osteoblastic differentiation.</div></div><div><h3>Conclusion</h3><div>Submandibular saliva has a biphasic effect on the osteogenic commitment of bone marrow progenitor cells, depending on the concentration and duration of exposure. This finding underscores the active role of saliva in the repair and regeneration of tooth sockets.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106484"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145776683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there geometric morphometric evidence for the selection against impaction? A comparative cross-sectional study of specific tooth-agenesis patterns","authors":"Sarah Boussali ,&nbsp;Anaïs Cavaré","doi":"10.1016/j.archoralbio.2025.106462","DOIUrl":"10.1016/j.archoralbio.2025.106462","url":null,"abstract":"<div><h3>Objectives</h3><div>Reductions in tooth number and facial prognathism may reflect shared genetic and evolutionary mechanisms but have been inconsistently reported by traditional cephalometric analyses. This study aimed to assess whether common patterns of dental agenesis are associated with craniofacial morphology using geometric morphometric methods.</div></div><div><h3>Design</h3><div>This retrospective comparative cross-sectional study included 538 patients aged 10–19 years from a French orthodontic population. Individuals with hypodontia (n = 269), restricted to third molars, maxillary lateral incisors, or second premolars, were matched by age and sex with controls without agenesis (n = 269). Craniofacial morphology was assessed on lateral cephalograms using 18 landmarks and 87 semilandmarks. Multiple linear regression and Procrustes MANOVA were applied to evaluate differences in size and shape, adjusting for sex and age.</div></div><div><h3>Results</h3><div>No significant association was found between agenesis status and craniofacial size in the overall configuration as well as in the cranial base, maxilla, and mandible. A significant sex effect was detected in the mandible, with smaller centroid size in males (<em>p</em> = 0.012). Multivariate regression on shape confirmed allometric effects across all configurations. Procrustes MANOVA detected a significant effect of sex on overall shape (p = 0.003), but neither age nor hypodontia reached significance.</div></div><div><h3>Conclusion</h3><div>In this large geometric morphometric study, no generalized or localized alterations in craniofacial morphology were detected, even in third molar agenesis, suggesting that the anthropological significance of hypodontia should be regarded with caution.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106462"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145566631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tetramethoxyluteolin, an active constituent in mulberry leaves, promotes osteogenesis of jaw bone marrow mesenchymal stem cells in periodontitis microenvironment via NF-κB inhibition","authors":"Yuning Xia ,&nbsp;Chao Shan ,&nbsp;Zeyu Wu ,&nbsp;Jin Zhao","doi":"10.1016/j.archoralbio.2025.106459","DOIUrl":"10.1016/j.archoralbio.2025.106459","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate the effects and mechanisms of tetramethoxyluteolin (TML), a bioactive compound in mulberry leaves, on jaw bone marrow mesenchymal stem cells (JBMMSCs) in a periodontitis microenvironment.</div></div><div><h3>Design</h3><div>Network pharmacology and molecular docking were used to identify mulberry leaves' active constituents and their targets in periodontitis treatment. An inflammatory model was established in JBMMSCs using <em>Porphyromonas gingivalis</em>-lipopolysaccharide (5 μg/mL). TML's optimal concentration was determined via CCK-8 and ELISA. Osteogenic differentiation, inflammatory markers, and NF-κB pathway activity were assessed using ALP/ARS staining, RT-PCR, and Western blot (WB). A rat model of ligature-induced periodontitis was established, and TML's effects were evaluated through histopathological staining, micro-CT, RT-PCR, and WB. JBMMSCs from each animal experimental group were isolated for in vitro osteogenic validation. Mechanisms were clarified by comparing TML with the NF-κB inhibitor BAY11–7082.</div></div><div><h3>Results</h3><div>TML was identified as the key constituent targeting NF-κB and inflammatory mediators (IL-6, IL-1β, TNF-α). 5 μM TML significantly suppressed inflammatory cytokines, promoted osteogenic differentiation, and inhibited NF-κB activation in JBMMSCs. In rats, 30 mg/kg TML markedly reduced inflammation and alveolar bone loss, showing efficacy comparable to indomethacin, and JBMMSCs from TML-treated groups exhibited enhanced osteogenesis. TML's inhibition of NF-κB was similar to BAY11–7082.</div></div><div><h3>Conclusion</h3><div>TML reduces periodontal inflammation and enhances the osteogenic potential of JBMMSCs by inhibiting the NF-κB pathway, providing a novel strategy for periodontitis-related bone regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106459"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145566620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical analysis of immune checkpoint proteins (PD-1, PD-L1 and PD-L2) in giant cell granulomas of the jaws and giant cell tumor of bone","authors":"Elton Fernandes Barros ,&nbsp;Vanessa Alves de Medeiros ,&nbsp;Éricka Janine Dantas da Silveira ,&nbsp;João Augusto Vianna Goulart Filho ,&nbsp;Pollianna Muniz Alves ,&nbsp;Cassiano Francisco Weege Nonaka","doi":"10.1016/j.archoralbio.2025.106460","DOIUrl":"10.1016/j.archoralbio.2025.106460","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the immunoexpression of programmed cell death protein 1 (PD-1) and programmed cell death ligands 1 (PD-L1) and 2 (PD-L2) in giant cell granulomas of the jaws (central giant cell granuloma [CGCG], peripheral giant cell granuloma [PGCG]) and giant cell tumor of bone (GCTB).</div></div><div><h3>Design</h3><div>Thirty CGCG (15 non-aggressive and 15 aggressive), 15 PGCG, and 15 GCTB were selected. The percentages of cytoplasmic (PD-1, PD-L1, and PD-L2) and nuclear (PD-L1) staining in mononuclear cells (MC) and in non-cannibalistic (ncMGC) and cannibalistic MGC (cMGC) were determined.</div></div><div><h3>Results</h3><div>Cytoplasmic expression of PD-1 and PD-L1 was observed in all groups, with high median percentages of positivity in ncMGC and cMGC. Compared to CGCG and PGCG, GCTB exhibited higher expression of PD-L1 in MC (<em>p</em> &lt; 0.05). In ncMGC, expression of PD-1 was higher in GCTB compared to non-aggressive CGCG (<em>p</em> &lt; 0.05). Similarly, higher PD-L1 immunopositivity was found in ncMGC of GCTB compared to aggressive CGCG and PGCG (<em>p</em> &lt; 0.05). For all cell types, lower median percentages of PD-L2 positivity were observed in GCTB compared to CGCG and PGCG (<em>p</em> &gt; 0.05). In GCTB, there was a strong positive correlation between the cytoplasmic expression of PD-L1 in MC and PD-1 in ncMGC (<em>r</em> = 0.535; <em>p</em> &lt; 0.05). All groups exhibited low nuclear immunoexpression of PD-L1.</div></div><div><h3>Conclusion</h3><div>The results suggest the potential participation of PD-1, PD-L1, and PD-L2 in the pathogenesis of CGCG, PGCG, and GCTB. The locally aggressive behavior of GCTB could be associated with a higher osteoclastogenic and immunosuppressive microenvironment in these neoplasms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106460"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145566592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Bifidobacterium longum, Lactobacillus rhamnosus, and Lactobacillus reuteri on oral microbial balance and host cell functions: Implications for the prevention and management of oral diseases","authors":"Zhigang Zhu ,&nbsp;Xiang Li ,&nbsp;Jianwei Liu ,&nbsp;Liang Chen ,&nbsp;Yutong Zhan ,&nbsp;Li Wang ,&nbsp;Luxian Zhou ,&nbsp;Dandan Jiang ,&nbsp;Xianwu Peng ,&nbsp;Xiuyu Jiang","doi":"10.1016/j.archoralbio.2025.106458","DOIUrl":"10.1016/j.archoralbio.2025.106458","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the effects of three probiotic samples—<em>Bifidobacterium longum</em>, <em>Lactobacillus rhamnosus</em>, and <em>Lactobacillus reuteri</em>—on oral pathogens, commensal bacteria, and host oral cells, thereby exploring their potential roles in maintaining microbial balance and promoting host defense.</div></div><div><h3>Design</h3><div>Probiotic culture supernatants (postbiotic fractions) were tested against oral pathogens (<em>S. mutans</em>, <em>P. gingivalis</em>, <em>F. nucleatum</em>, <em>A. actinomycetemcomitans</em>) and commensal species (<em>S. sanguinis</em>, <em>V. parvula</em>) using optical density assays. Primary human gingival fibroblasts and oral keratinocytes were co-cultured with probiotic preparations to assess cell viability, inflammatory cytokine secretion, and barrier gene expression (<em>FLG</em>, <em>LOR</em>).</div></div><div><h3>Results</h3><div><em>B. longum, L. rhamnosus, and L. reuteri</em> samples showed strain- and concentration-dependent inhibition of oral pathogens, while exhibiting minimal effects on beneficial commensals. Appropriate concentrations of probiotic samples preserved fibroblast viability and enhanced keratinocyte survival. Notably, <em>B. longum</em>, <em>L. rhamnosus</em>, and <em>L. reuteri</em> upregulated barrier-associated genes (<em>FLG</em>, <em>LOR</em>) and suppressed IL-6 secretion in inflamed keratinocytes, suggesting immunomodulatory and protective roles.</div></div><div><h3>Conclusions</h3><div>Probiotic-derived metabolites exert selective antimicrobial and cytoprotective effects in the oral microenvironment. These findings support the dual functions of probiotics in reshaping oral microbial balance and modulating host cell functions and highlight the potential for personalized probiotic strategies in oral disease prevention and management.Further exploration of combined probiotic formulations is warranted to optimize clinical translation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106458"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145577799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}