Archives of oral biology最新文献

{"title":"Palatal morphology: A systematic review of the association of palatal shape with genetic ancestry, sex and age","authors":"Thao Liang Chiam ,&nbsp;Harry Perkins ,&nbsp;Toby Hughes ,&nbsp;Lyle Palmer ,&nbsp;Denice Higgins","doi":"10.1016/j.archoralbio.2025.106275","DOIUrl":"10.1016/j.archoralbio.2025.106275","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to review the literature regarding palatal shape variation, and its influence on estimating genetic ancestry, sex, and age.</div></div><div><h3>Design</h3><div>A literature search was performed using PubMed, Scopus and Embase databases, from inception up to January 2025. Original research articles in English examining variation in shape of human hard palate were included, irrespective of study type or publication year. Articles regarding craniofacial anomalies, pathologies and orthodontic treatment were excluded. The risk of bias was assessed independently by two reviewers using the Joanna Briggs Institute critical appraisal tool.</div></div><div><h3>Results</h3><div>Twenty-two studies fulfilled the selection criteria. Dental casts (<em>n</em> = 13) and crania (<em>n</em> = 8) were predominantly utilised in the studies. Geometric morphometrics emerged as the predominant method for palatal shape analysis, followed by fourth-order polynomial curve fitting, visual assessments and ratios. Five studies examined ancestral variation in palatal shape, with four reporting differences between population groups. These studies, however, utilised fixed landmarks only, offering limited insight. Sexual dimorphism of palatal shape was extensively investigated (<em>n</em> = 12), with large numbers of semi-landmarks employed in two studies, but association was reported in four studies only. Palatal shape was reported to change from birth to adulthood but remained unchanged after adulthood.</div></div><div><h3>Conclusion</h3><div>This review highlights that palatal shape may aid ancestry classification but remains inconsistent to classify sex. Palatal shape changes during growth are allometric and hence need to be considered in forensic investigations. More standardised and robust methods may better evaluate the effects of ancestry, sex and age on 3D palatal variation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106275"},"PeriodicalIF":2.2,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the role of Th17 cell and related biomarkers in periodontitis: A systematic review","authors":"Sangamithra Sidharthan ,&nbsp;Gopalakrishnan D ,&nbsp;Supriya Kheur ,&nbsp;Subhashree Mohapatra","doi":"10.1016/j.archoralbio.2025.106272","DOIUrl":"10.1016/j.archoralbio.2025.106272","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the evidence for presence of Th17 cells and their biomarkers, and to assess their impact on the immune-inflammatory response in periodontitis.</div></div><div><h3>Materials and methods</h3><div>An electronic search was performed in MEDLINE (PubMed), SCOPUS, EBSCOhost, and Google Scholar databases from their earliest records to April 2023. Additionally, the reference lists of included articles and grey literature were hand-searched. Study selection and quality assessment of the included articles was performed using the Newcastle-Ottawa scale.</div></div><div><h3>Results</h3><div>This systematic review included case-control, cross-sectional, and cohort studies published in English, specifically those evaluating the presence and influence of Th17 or its related biomarkers in the progression of periodontal disease. Of the 26,797 articles screened, 47 studies met the eligibility criteria and were included. The studies varied in design, molecular methods, and sample types.</div></div><div><h3>Conclusion</h3><div>This systematic review confirms the presence of Th17 cells and related biomarkers in periodontal tissues, highlighting their role in the immune-inflammatory response and pathogenesis of periodontitis. The review underscores the need for more comprehensive research to overcome current limitations and effectively translate these findings into clinical practice.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106272"},"PeriodicalIF":2.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143934861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential expression reveals inflammatory response and oxidative stress genes in dentin caries","authors":"Simone G. Oliveira ,&nbsp;Rodrigo Jardim ,&nbsp;Nelson Kotowski ,&nbsp;Alberto M.R. Dávila ,&nbsp;Hélio R. Sampaio-Filho ,&nbsp;Karina G.S. Ruiz ,&nbsp;Flávio H.B. Aguiar","doi":"10.1016/j.archoralbio.2025.106274","DOIUrl":"10.1016/j.archoralbio.2025.106274","url":null,"abstract":"<div><h3>Objective</h3><div>This study employs RNA-Seq to investigate differentially expressed genes involved in extracellular matrix (ECM) degradation, focusing on collagenases (MMP-2 and MMP-9) and their inhibitors (TIMP-1 and TIMP-2).</div></div><div><h3>Design</h3><div>Total RNA from caries and caries-free teeth was extracted from pulp, predentin, and dentin. Samples were sequenced using Illumina® technology. Quality validation was done with FASTQC, and low-quality bases were removed using TRIMMOMATIC. Reads were aligned using SALMON against the human transcriptome (CHR38), followed by quantification using Transcripts Per Million. Differential gene expression analysis was conducted using DESeq2 (FDR &lt; 0.05, |log2FC| ≥ 1). Functional enrichment analyses employed Gene Ontology and KEGG databases.</div></div><div><h3>Results</h3><div>Sequencing produced 16–37 million reads per sample, with an average alignment rate of 88.08 %. A total of 334 differentially expressed genes (DEGs) were identified: 195 upregulated and 139 downregulated. Upregulated genes included SAA1 (log2FC = 2.3, p-adj = 0.001) and ORM1 (log2FC = 2.0, p-adj = 0.002), associated with inflammation. MMP-9 was significantly downregulated (log2FC = −1.8, p-adj = 0.003), while MMP-2 showed higher expression in decayed tissues. TIMP-1 expression increased in decayed dentin; TIMP-2 was upregulated in both decayed and caries-free dentin. Protein interaction analysis identified EGFR and metallothioneins as key acute-phase proteins.</div></div><div><h3>Conclusions</h3><div>This study reveals the role of inflammatory and oxidative stress-related genes in dentin caries and shows disruption in the ECM degradation-repair balance. Increased MMP-2 and TIMP-1 expression suggests a compensatory response. MMP activity may serve as a therapeutic target to enhance tissue resilience and slow caries progression.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106274"},"PeriodicalIF":2.2,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of recombinant human sclerostin on proliferation and migration in human cementoblast lineage cells","authors":"Naonobu Okimura ,&nbsp;Ryo Kunimatsu ,&nbsp;Isamu Kado ,&nbsp;Ayaka Nakatani ,&nbsp;Shuzo Sakata ,&nbsp;Kazutaka Ikeda ,&nbsp;Shota Ito ,&nbsp;Tomohiro Ogasawara ,&nbsp;Shintaro Ogashira ,&nbsp;Mutsumi Miyauchi ,&nbsp;Takashi Takata ,&nbsp;Kotaro Tanimoto","doi":"10.1016/j.archoralbio.2025.106273","DOIUrl":"10.1016/j.archoralbio.2025.106273","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of sclerostin on the proliferation and migration of human cementoblasts and periodontal ligament cells.</div></div><div><h3>Design</h3><div>Sclerostin expression in human cementoblasts and periodontal ligament cells was assessed using immunochemical staining. Human cementoblasts and periodontal ligament cells were cultured and treated with 100 ng/mL of recombinant human sclerostin. Cell proliferation was evaluated using a 5-bromo-2-deoxyuridine enzyme-linked immunosorbent assay and quantified with a live-cell imaging and analysis platform (IncuCyte® S3 system). Furthermore, sclerostin’s impact on apoptosis in human cementoblasts and periodontal ligament cells was evaluated using IncuCyte® Caspase-3/7 green dye. Additionally, cell migration was analyzed through quantitative wound healing assessment using the IncuCyte® S3 system. Polymerase chain reaction, enzyme-linked immunosorbent assay, and Western blotting were then performed to confirm the effect of sclerostin on CEMP-1. The data obtained were statistically analyzed using the Mann–Whitney U test.</div></div><div><h3>Results</h3><div>Intracellular sclerostin localization in human cementoblasts and periodontal ligament cells were confirmed form the immunochemical staining. The sclerostin-treated group showed suppressed proliferation and migration of human cementoblasts and periodontal ligament cells compared with the non-treated group. Furthermore, the sclerostin-treated group showed significantly elevated caspase-3/7 activity compared with the non-treated group. However, the addition of sclerostin did not result in any significant changes in CEMP-1.</div></div><div><h3>Conclusion</h3><div>Sclerostin is crucial in regulating the proliferation and migration of cementoblasts and periodontal ligament cells. This highlights its importance in regenerating the cementum and periodontal ligament.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106273"},"PeriodicalIF":2.2,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143877474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Potential impact of micro-196a2 and Toll-like receptor 2 gene polymorphisms on oral cancer prognosis and susceptibility","authors":"Marko Simonovic ,&nbsp;Debora Misic ,&nbsp;Ruzica Kozomara ,&nbsp;Aleksandra Petkovic Curcin ,&nbsp;Sasa Jovic ,&nbsp;Marko Brkic ,&nbsp;Dragan Pandzic ,&nbsp;Srboljub Stosic ,&nbsp;Gordana Supic","doi":"10.1016/j.archoralbio.2025.106271","DOIUrl":"10.1016/j.archoralbio.2025.106271","url":null,"abstract":"<div><h3>Objective</h3><div>Despite advances in diagnosis and treatment in recent years, oral squamous cell carcinoma (OSCC) is still associated with a high recurrence rate and poor survival. MiR-196a2 and TLR2 have been implicated in cancer progression and prognosis, but the significance of their genetic variants in OSCC remains unelucidated.</div></div><div><h3>Design</h3><div>This study investigated the <em>miR-196a2</em> rs11614913 and <em>TLR2</em> rs5743708 genetic variants in Caucasian HPV-negative OSCC patients (n = 95) and age- and sex-matched healthy controls (n = 108) using real-time PCR. An assessment was conducted on their association with clinicopathological features, overall survival (OS), recurrence-free survival (RFS) and OSCC risk.</div></div><div><h3>Results</h3><div>OSCC patients carrying the <em>miR-196a2</em> rs11614913 TT genotype had a higher risk of tumor recurrence (P = 0.045) and shorter RFS (P = 0.041). The proportional hazards assumption was violated for tumor stage. Stage-stratified Kaplan-Meier analysis showed that <em>miR-196a2</em> rs11614913 genotypes and combined CC+CT vs. TT variants significantly affected RFS in stage I/II OSCC patients (<em>P</em> = 0.012 and <em>P</em> = 0.003, respectively), but not in advanced stage III/IV patients (<em>P</em> = 0.545 and <em>P</em> = 0.287, respectively). Cox regression confirmed <em>miR-196a2</em> rs11614913 as an independent predictor of RFS in early stage (HR=3.407, <em>P</em> = 0.015), but not in advance stage patients (HR=1.090, <em>P</em> = 0.711). No significant associations with OS were found. Additionally, the <em>TLR2</em> rs5743708 variant allele A was significantly associated with a lower risk of OSCC (Adjusted OR=0.406, P = 0.013).</div></div><div><h3>Conclusions</h3><div>These findings suggest that <em>miR-196a2</em> rs11614913 could play a stage-dependent role in RFS, influencing early-stage OSCC but losing prognostic significance in advanced disease. Additionally, <em>TLR2</em> rs5743708 may contribute to the decreased OSCC risk.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106271"},"PeriodicalIF":2.2,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic and phenotypic heterogeneity of tooth agenesis: An update including candidate genes","authors":"Wantao Li ,&nbsp;Wenjing Xu","doi":"10.1016/j.archoralbio.2025.106270","DOIUrl":"10.1016/j.archoralbio.2025.106270","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to further investigate the genetic etiology as well as the corresponding characteristics of tooth agenesis (TA). It focuses on expanding the gene spectrum and exploring genotype-phenotype correlations and potential candidate genes for TA.</div></div><div><h3>Design</h3><div>The narrative review approach was conducted, providing a comprehensive perspective of tooth agenesis-related literature.</div></div><div><h3>Results</h3><div>We describe the (candidate) causal genes of syndromic TA and nonsyndromic TA respectively. There is overlap between the gene spectrum of the two forms. Tooth phenotypes (either the number of missing teeth or the malformations) of syndromic form are more severe than that of nonsyndromic form. The phenomenon even exists among family members carried the same variant, highlighting the disorder’s complexity and the causal genes’ expression variability. Besides, the candidate genes, corresponding functional and case evidence are updated, which contributes to improve the diagnosis of TA.</div></div><div><h3>Conclusions</h3><div>TA is a group of complex disorder regulated by multiple genetic signaling pathways. We review the previously known and novel found genes/candidate genes related to TA, emphasizing the genetic and phenotypic heterogeneity. The enlarged spectrum is useful for further promoting the understanding of TA and early diagnosis. It is suggested that molecular diagnosis is particularly vital for early management and genetic counseling.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106270"},"PeriodicalIF":2.2,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143843050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of azurocidin and its implications in periodontal and peri-implant disease: A systematic review","authors":"Mario Alberto Alarcón-Sánchez ,&nbsp;Sarah Monserrat Lomelí-Martínez ,&nbsp;Ruth Rodríguez-Montaño ,&nbsp;Julieta Sarai Becerra-Ruiz ,&nbsp;Cristina Hermila Martínez-Bugarin ,&nbsp;Seyed Ali Mosaddad ,&nbsp;Artak Heboyan","doi":"10.1016/j.archoralbio.2025.106256","DOIUrl":"10.1016/j.archoralbio.2025.106256","url":null,"abstract":"<div><h3>Objectives</h3><div>This systematic review aimed to explore the role of Azurocidin (Azu) in the pathogenesis of periodontal and peri-implant disease and its potential use as an inflammatory biomarker.</div></div><div><h3>Materials and methods</h3><div>Four electronic databases were used for study identification: PubMed, Google Scholar, ScienceDirect, and Scopus from Oct 10, 1991 to Jul 15, 2024. Study selection and data extraction were performed in a blinded and independent manner. The Joanna Briggs Institute (JBI) tool was used to assess the quality of cross-sectional articles, and the Newcastle-Ottawa scale was used to assess cohort studies<em>.</em></div></div><div><h3>Results</h3><div>Out of 222 identified articles, nine studies met the inclusion criteria. These studies included 462 participants: 156 with healthy teeth and implants and 306 with periodontal conditions such as gingivitis, periodontitis, apical periodontitis, peri-implant mucositis, and peri-implantitis. A total of 1313 samples were analyzed (163 saliva, 118 PICF, 1003 GCF, 11 gingival tissue, and 18 infected root canals). ELISA was the most common method for azurocidin analysis (66.6 %), followed by LC-MS/MS (33.3 %), nLC-MS/MS (11.1 %), and Western Blot (11.1 %). Azu levels were consistently elevated in individuals with periodontitis compared to periodontally healthy subjects.</div></div><div><h3>Conclusions</h3><div>Azu may contribute to the inflammatory processes in periodontal and peri-implant diseases. Although elevated levels are observed in periodontitis, its diagnostic value remains unclear due to limited and heterogeneous data.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106256"},"PeriodicalIF":2.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen peroxide-induced oxidative stress alters protein expression in two rat salivary acinar cell lines","authors":"Golnaz Golnarnik ,&nbsp;Bernd Thiede ,&nbsp;Tine M. Søland ,&nbsp;Hilde K. Galtung ,&nbsp;Trude M. Haug","doi":"10.1016/j.archoralbio.2025.106254","DOIUrl":"10.1016/j.archoralbio.2025.106254","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate the impact of hydrogen peroxide-induced oxidative stress on the protein expression profiles of submandibular and parotid acinar cells using a proteomic approach. We sought to evaluate how oxidative stress might contribute to salivary gland dysfunction and whether the two glands respond differently.</div></div><div><h3>Design</h3><div>Immortalized rat parotid gland (PG) and submandibular gland (SMG) acinar epithelial cell lines were exposed to 50 µM and 150 µM hydrogen peroxide for 24 hr, followed by protein identification and quantification via liquid chromatography-mass spectrometry. Immunofluorescence microscopy and western blot analysis validated selected protein expressions, and cell viability was assessed using trypan blue exclusion assays.</div></div><div><h3>Results</h3><div>Compared to controls, histone H4 expression increased in both cell types after hydrogen peroxide exposure, whereas voltage-dependent anion-selective channel 1, keratin 7, and keratin 8 increased only in parotid gland cells. Conversely, mitochondrial aldehyde dehydrogenase and kidney isoform glutaminase were downregulated in parotid gland cells. Basal expression of mitochondrial aldehyde dehydrogenase and catalase was higher in submandibular gland cells. At higher hydrogen peroxide concentrations, antioxidant proteins expression and cell viability were greater in submandibular gland cells compared to parotid gland cells.</div></div><div><h3>Conclusions</h3><div>Our results suggest that submandibular gland acinar cells exhibit greater resistance to oxidative stress compared to parotid gland cells, potentially due to distinct antioxidant and metabolic coping strategies. Understanding these gland-specific responses may contribute to future approaches to protect salivary glands from oxidative damage under pathological conditions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106254"},"PeriodicalIF":2.2,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143825397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sitagliptin regulates the AMPK/NF-κB signaling pathway to alleviate lipopolysaccharide-induced inflammatory responses and promote osteogenic differentiation in rat bone marrow mesenchymal stem cells","authors":"Xi Yao ,&nbsp;Min Liu ,&nbsp;Pin Wang","doi":"10.1016/j.archoralbio.2025.106253","DOIUrl":"10.1016/j.archoralbio.2025.106253","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the impact of sitagliptin on the inflammatory response and osteogenic differentiation in lipopolysaccharide (LPS)-stimulated rat bone marrow mesenchymal stem cells (BMSCs) and to clarify the underlying mechanisms of action.</div></div><div><h3>Design</h3><div><em>In vitro-</em>cultured rat BMSCs were identified, treated with a range of sitagliptin doses, and assessed with a cell counting kit-8 assay to quantify viability. The expression of proteins and genes relevant to inflammation and osteogenesis was measured using enzyme-linked immunosorbent assay and real-time quantitative polymerase chain reaction techniques. The osteogenic differentiation ability of rat BMSCs was analyzed by alkaline phosphatase staining, alkaline phosphatase activity assay, and alizarin red s staining. Adenosine monophosphate-activated protein kinase (AMPK)/nuclear factor-kappa B (NF-κB) signaling pathway activation was detected through western blotting.</div></div><div><h3>Results</h3><div>High but not low sitagliptin concentrations significantly suppressed cellular viability. Sitagliptin dose-dependently inhibited LPS-induced rat BMSCs inflammatory responses while facilitating their osteogenic differentiation. It also activated AMPK pathway signaling while suppressing NF-κB activity. AMPK inhibitor treatment of rat BMSCs partially reversed these beneficial effects of sitagliptin on inflammation and osteogenesis.</div></div><div><h3>Conclusions</h3><div>Sitagliptin suppresses rat BMSCs inflammation while promoting osteogenesis through the modulation of AMPK/NF-κB signaling activity, thereby mitigating the functional impairment of rat BMSCs under inflammatory microenvironmental conditions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106253"},"PeriodicalIF":2.2,"publicationDate":"2025-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of intratumor dendritic cell vaccination with and without chemoradiation in induced oral squamous cell carcinoma of hamsters","authors":"Nouran Mohamed Amr ,&nbsp;Mohamed Labib Salem ,&nbsp;Mohamed Monai Shoshan ,&nbsp;Basant Hamdy Abouzaid","doi":"10.1016/j.archoralbio.2025.106252","DOIUrl":"10.1016/j.archoralbio.2025.106252","url":null,"abstract":"<div><h3>Objectives</h3><div>Oral squamous cell carcinoma represents an important health problem since its increasing incidence and persistent high mortality rates. The traditional therapies remain surgery and chemoradiation which have shown limited success in treating recurrent and metastatic cancers with multiple complications. Immunotherapy using dendritic cell vaccination is a rather novel approach that, yet, showed limited therapeutic efficacy in eradicating oral squamous cell carcinoma since the latter is known for its immunosuppressive microenvironment. Hence, there is pressing demand for adopting new multimodality approaches that would augment the antitumor effect of dendritic cell vaccine with lesser side effects and no relapse.</div></div><div><h3>Design</h3><div>Chemically induced oral squamous cell carcinoma model of hamster buccal pouch using 7,12-Dimethylbenz[<em>a</em>]anthracene was established. The clinically evident tumor masses were then 75 % surgically debulked. Resected tumors were manipulated to prepare dendritic cell vaccine. Animals were divided into 3 groups and subsequently subjected to different treatment approaches.</div></div><div><h3>Results</h3><div>The combination therapy of debulking surgery, chemoradiation, and intratumor dendritic cell vaccination significantly delayed tumor growth with complete regression of induced tumors. Moreover, this modality was associated with the highest survival rate (89 %) with establishment of immunological memory.</div></div><div><h3>Conclusions</h3><div>Our findings emphasize the importance of modifying post-surgical environment, via co-administration of adjuvant chemoradiation, in generating robust anti-tumor immunologic activity. Chemoradiation and dendritic cell vaccines are available for clinical use/trial which endows this approach the merit of potential clinical application.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106252"},"PeriodicalIF":2.2,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}