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OIP5-AS1 expression profiles in different stages of oral squamous cell carcinoma OIP5-AS1在口腔鳞状细胞癌不同分期中的表达谱
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-24 DOI: 10.1016/j.archoralbio.2025.106403
Ameya K.P., Ashikha Shirin Usman P.P., Durairaj Sekar
{"title":"OIP5-AS1 expression profiles in different stages of oral squamous cell carcinoma","authors":"Ameya K.P.,&nbsp;Ashikha Shirin Usman P.P.,&nbsp;Durairaj Sekar","doi":"10.1016/j.archoralbio.2025.106403","DOIUrl":"10.1016/j.archoralbio.2025.106403","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the expression patterns of long non-coding RNA OIP5-AS1 across various stages of oral squamous cell carcinoma (OSCC) and assess its potential as a biomarker and therapeutic target.</div></div><div><h3>Design</h3><div>A comprehensive review of recent literature focusing on OIP5-AS1's role in OSCC was conducted. Analysis included OIP5-AS1 expression levels in cancerous versus non-cancerous tissues and exploration of its interactions with tumor-suppressing microRNAs (miRNAs).</div></div><div><h3>Results</h3><div>OIP5-AS1 was found to be significantly upregulated in advanced stages of OSCC compared to non-cancerous tissues. Its function as a molecular sponge for miRNAs contributes to the promotion of tumorigenic pathways, complicating therapeutic responses and highlighting its role as an oncogene.</div></div><div><h3>Conclusions</h3><div>OIP5-AS1 is a critical player in the progression of OSCC, influencing tumor dynamics and mechanisms of resistance. Elucidating its expression patterns and functional roles suggests that OIP5-AS1 could serve as a valuable biomarker for early diagnosis and personalized treatment strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106403"},"PeriodicalIF":2.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular and thermal signatures of dental tissues in third molars: An in vitro comparative study using fourier-transform infrared spectroscopy and differential scanning calorimetry 第三磨牙牙组织的分子和热特征:傅里叶变换红外光谱和差示扫描量热法的体外比较研究。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-19 DOI: 10.1016/j.archoralbio.2025.106402
Rola Zahedah , Recep Üstünsoy , Aliye Tuğçe Gürcan , Bircan Dinç
{"title":"Molecular and thermal signatures of dental tissues in third molars: An in vitro comparative study using fourier-transform infrared spectroscopy and differential scanning calorimetry","authors":"Rola Zahedah ,&nbsp;Recep Üstünsoy ,&nbsp;Aliye Tuğçe Gürcan ,&nbsp;Bircan Dinç","doi":"10.1016/j.archoralbio.2025.106402","DOIUrl":"10.1016/j.archoralbio.2025.106402","url":null,"abstract":"<div><h3>Objective</h3><div>To characterize and compare the molecular and thermal characteristics of enamel, dentin, cementum, and the dentin–pulp complex in permanent third molars using Fourier-transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC).</div></div><div><h3>Design</h3><div>Samples from extracted third molars (n = 15) were prepared and analyzed using FTIR to assess molecular composition and DSC to evaluate thermal transitions, including dehydration, collagen degradation, and mineral phase transformation. All measurements were conducted in triplicate.</div></div><div><h3>Results</h3><div>FTIR revealed enamel as highly mineralized with minimal organic content, dentin and cementum as collagen-rich, and the dentin–pulp complex as a hybrid tissue. DSC analysis identified consistent thermal transitions: water loss (110–125 °C), collagen breakdown (300–320 °C), and mineral decomposition (455–470 °C). Enamel displayed the highest crystallinity, while cementum exhibited the highest enthalpy change. Tissues with stronger FTIR collagen peaks corresponded to higher DSC energy release during protein degradation.</div></div><div><h3>Conclusion</h3><div>Molecular and thermal profiling of dental tissues provide baseline reference data for biomaterial design and regenerative strategies.</div></div><div><h3>Clinical significance</h3><div>Understanding tissue-specific molecular and thermal properties can guide the development of biomimetic restorative materials, inform safer thermal thresholds during clinical procedures, and support diagnostic approaches for aging and pathological changes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106402"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Immunohistochemical assessment of murine double minute 2 in solid vs unicystic ameloblastoma: A systematic review and meta-analysis 小鼠双分钟2在实体型和单囊型成釉细胞瘤中的免疫组织化学评价:系统回顾和荟萃分析
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-19 DOI: 10.1016/j.archoralbio.2025.106400
Lilibeth-Stephania Escoto-Vasquez , Mario Alberto Alarcón-Sánchez , Julieta Sarai Becerra-Ruiz , Cristina Hermila Martínez-Bugarin , Sarah Monserrat Lomelí-Martínez , Armen A. Muradyan , Artak Heboyan
{"title":"Immunohistochemical assessment of murine double minute 2 in solid vs unicystic ameloblastoma: A systematic review and meta-analysis","authors":"Lilibeth-Stephania Escoto-Vasquez ,&nbsp;Mario Alberto Alarcón-Sánchez ,&nbsp;Julieta Sarai Becerra-Ruiz ,&nbsp;Cristina Hermila Martínez-Bugarin ,&nbsp;Sarah Monserrat Lomelí-Martínez ,&nbsp;Armen A. Muradyan ,&nbsp;Artak Heboyan","doi":"10.1016/j.archoralbio.2025.106400","DOIUrl":"10.1016/j.archoralbio.2025.106400","url":null,"abstract":"<div><h3>Objective</h3><div>This project aimed to evaluate the immunoexpression pattern of murine double minute 2 (MDM2) in solid ameloblastomas compared to unicystic ameloblastomas.</div></div><div><h3>Methods</h3><div>The review followed PRISMA guidelines and was registered in the PROSPERO database. PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar were comprehensively searched. Original cross-sectional studies were included. The meta-analysis was performed using STATA V15 and RevMan. Positivity rates were pooled using a random-effects model (REM), the labeling index was analyzed using mean difference under a REM, and expression intensity (moderate–strong) was assessed as categorical data using a REM with Hartung-Knapp adjustment. Heterogeneity was evaluated with the Chi² test and I² statistic. The methodological quality and certainty of the evidence were assessed using the Joanna Briggs Institute items and the GRADE system.</div></div><div><h3>Results</h3><div>Nine studies (n = 438 specimens) were analyzed, of which 325/438 (74.2 %) were ameloblastoma biopsies. MDM2 positivity was detected in 403/438 cases (92 %). A statistically significant association in favor of solid ameloblastoma was observed (RR = 2.08; 95 %CI [1.66–2.60]; p = 0.005), indicating a twofold probability of finding high MDM2 expression in solid compared to unicystic ameloblastomas. Four of the nine studies (44.4 %) were considered to be of low quality, and the certainty of evidence was low to very low.</div></div><div><h3>Conclusions</h3><div>MDM2 expression was prevalent in both types of ameloblastomas, with a higher intensity of expression observed in solid cases. However, due to study heterogeneity, further investigations with more robust methodological designs are recommended to assess the diagnostic potential of MDM2 in ameloblastomas.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106400"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Editorial on the special issue on temporomandibular disorders (TMD), AOB journal 关于颞下颌疾病(TMD)特刊的社论,AOB杂志
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-19 DOI: 10.1016/j.archoralbio.2025.106401
Meiqing Wang , Dongmei He
{"title":"Editorial on the special issue on temporomandibular disorders (TMD), AOB journal","authors":"Meiqing Wang ,&nbsp;Dongmei He","doi":"10.1016/j.archoralbio.2025.106401","DOIUrl":"10.1016/j.archoralbio.2025.106401","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106401"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
ZBP1-NLRP3 axis integrates PANoptosis and ferroptosis during inflammatory injury in human dental pulp fibroblasts ZBP1-NLRP3轴整合了人牙髓成纤维细胞炎症损伤过程中的PANoptosis和ferroptosis。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-19 DOI: 10.1016/j.archoralbio.2025.106398
Ai-E. He , Xing Wang , Ni Xie, Yun-He Xiao
{"title":"ZBP1-NLRP3 axis integrates PANoptosis and ferroptosis during inflammatory injury in human dental pulp fibroblasts","authors":"Ai-E. He ,&nbsp;Xing Wang ,&nbsp;Ni Xie,&nbsp;Yun-He Xiao","doi":"10.1016/j.archoralbio.2025.106398","DOIUrl":"10.1016/j.archoralbio.2025.106398","url":null,"abstract":"<div><h3>Objective</h3><div>To define how Z-DNA binding protein 1 (ZBP1) and NOD-like receptor family pyrin domain-containing 3 (NLRP3) signaling regulate lipopolysaccharide (LPS)-induced inflammation, PANoptosis, and ferroptosis in human dental pulp fibroblasts (HDPFs).</div></div><div><h3>Design</h3><div>HDPFs were treated with LPS, and <em>ZBP1</em> and <em>NLRP3</em> were silenced using small interfering RNA (siRNA), individually or in combination. Inflammatory mediators and death-pathway markers were quantified by quantitative real-time PCR (qRT-PCR), Western blotting, enzyme-linked immunosorbent assay (ELISA), and biochemical assays; Annexin V/propidium iodide flow cytometry assessed cell-death distributions.</div></div><div><h3>Results:</h3><div>LPS significantly increased ZBP1 and NLRP3 expression and elevated cytokine/chemokine release; each was attenuated by <em>ZBP1</em> or <em>NLRP3</em> knockdown, with the greatest reduction after dual silencing. LPS triggered PANoptosis, as indicated by increased Annexin V⁺/PI⁺ cell populations and upregulation of caspase-1, cleaved caspase-8, RIPK3, GSDMD, and p-MLKL/MLKL, which were significantly reduced by inhibition of the ZBP1-NLRP3 axis. Ferroptosis features were also evident after LPS, including impaired iron homeostasis (downregulated ferritin heavy chain 1 [FTH1] and ferroportin [FPN1] with Fe²⁺ accumulation), enhanced lipid peroxidation (upregulated ALOX15, LPCAT3, PTGS2 with increased malondialdehyde and lipid reactive oxygen species), and weakened antioxidant defenses (reduced glutathione peroxidase-4 [GPX4], solute carrier family 7 member 11 [SLC7A11], glutathione, and GPX4 activity). These changes were mitigated by single-gene silencing and most effectively by dual knockdown.</div></div><div><h3>Conclusion</h3><div>The ZBP1-NLRP3 axis acts upstream to coordinate LPS-induced PANoptosis and ferroptosis in HDPFs. Targeting this axis dampens inflammatory cell death and oxidative-metabolic dysregulation, highlighting a potential therapeutic strategy for pulpitis-related tissue injury.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106398"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Antimicrobial effect of cinnamaldehyde in a peri-implant mucositis model 肉桂醛在种植体周围黏膜炎模型中的抗菌作用。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-17 DOI: 10.1016/j.archoralbio.2025.106397
Verônica Cabral dos Santos Cunha D'Assunção , Maria Heloísa de Souza Borges-Grisi , Isis Morais Bezerra Muniz , Francisco Naldo Gomes Filho , Camilla Freire de Brito Bastos , Luís Fellipe Alves Silva , Francisco Humberto Xavier-Júnior , Rodrigo Othávio de Assunção e Souza , Leopoldina de Fátima Dantas de Almeida
{"title":"Antimicrobial effect of cinnamaldehyde in a peri-implant mucositis model","authors":"Verônica Cabral dos Santos Cunha D'Assunção ,&nbsp;Maria Heloísa de Souza Borges-Grisi ,&nbsp;Isis Morais Bezerra Muniz ,&nbsp;Francisco Naldo Gomes Filho ,&nbsp;Camilla Freire de Brito Bastos ,&nbsp;Luís Fellipe Alves Silva ,&nbsp;Francisco Humberto Xavier-Júnior ,&nbsp;Rodrigo Othávio de Assunção e Souza ,&nbsp;Leopoldina de Fátima Dantas de Almeida","doi":"10.1016/j.archoralbio.2025.106397","DOIUrl":"10.1016/j.archoralbio.2025.106397","url":null,"abstract":"<div><h3>Objective</h3><div>Evaluate the antimicrobial effect of an experimental mouthwash containing 1 % cinnamaldehyde in a polymicrobial biofilm model for peri-implant mucositis.</div></div><div><h3>Materials and methods</h3><div>Polymicrobial biofilms were seeded on zirconia and on titanium (n = 6 or 8/group) surfaces from stimulated saliva collection from 2 periodontal health donors and 2 with gingivitis. Salivary pellicle was performed, by 60 min. Inoculum was seeded (1 ×10<sup>8</sup> CFU/mL) in McBain medium (1 % of sucrose). Biofilms on zirconia were cultured in aerobiosis and on titanium in microaerophilic conditions for 24 h. Daily challenges with 10 % sucrose were stablished. Samples were exposed to the experimental mouthwash for 1 min, twice a day, for 72 h. Chlorhexidine digluconate at 0.12 % and saline solution (at 0.9 %) were used as controls. Biofilms were kept in culture for an additional 24 h to count viable cells, evaluate biomass and cellular metabolism.</div></div><div><h3>Results</h3><div>In zirconia, evaluation of cellular metabolism did not detect a statistically significant difference between the biofilm conditions (p &gt; 0.05). It was found that cellular metabolism was decreased from exposure to mouthwash and chlorhexidine, with a difference compared to the negative control (p &lt; 0.05). In titanium, viable cell count in two media and the biomass were higher for biofilms from the periodontal health salivary condition (p &lt; 0.05). There was a decrease in the biomass of biofilms exposed to the experimental mouthwash for both salivary conditions. Metabolic activity was decreased when exposed to experimental mouthwash and chlorhexidine compared to the negative control (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Experimental mouthwash with 1 % cinnamaldehyde showed an antimicrobial effect similar to chlorhexidine against polymicrobial biofilms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106397"},"PeriodicalIF":2.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Triggering mitotic catastrophe by podophyllotoxin induces apoptosis in oral squamous cell carcinoma 鬼臼毒素诱发有丝分裂突变诱导口腔鳞癌细胞凋亡。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-15 DOI: 10.1016/j.archoralbio.2025.106396
Su-Jung Choi , Ji-Hoon Kim , Hyun-Ji Kim , Dong-Guk Park , Bohwan Jin , Won Woo Lee , Kyoung-Ok Hong , Sak Lee , Thantrira Porntaveetus , Jae-Jin Cho , Seong-Doo Hong , Sung-Dae Cho
{"title":"Triggering mitotic catastrophe by podophyllotoxin induces apoptosis in oral squamous cell carcinoma","authors":"Su-Jung Choi ,&nbsp;Ji-Hoon Kim ,&nbsp;Hyun-Ji Kim ,&nbsp;Dong-Guk Park ,&nbsp;Bohwan Jin ,&nbsp;Won Woo Lee ,&nbsp;Kyoung-Ok Hong ,&nbsp;Sak Lee ,&nbsp;Thantrira Porntaveetus ,&nbsp;Jae-Jin Cho ,&nbsp;Seong-Doo Hong ,&nbsp;Sung-Dae Cho","doi":"10.1016/j.archoralbio.2025.106396","DOIUrl":"10.1016/j.archoralbio.2025.106396","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the relationship between mitotic catastrophe (MC) and apoptosis in oral squamous cell carcinoma (OSCC) using podophyllotoxin (PPT), a natural compound with antimitotic properties.</div></div><div><h3>Design</h3><div>We evaluated the concentration-dependent effects of PPT on cell proliferation (CCK-8 and soft agar assays) and morphology (transmission electron microscopy). Mechanistic insights were obtained by assessing DNA damage (western blotting), cell cycle progression (sub-G<sub>1</sub> analysis), and apoptosis-related protein activation in both 2D and 3D spheroid models of HSC-3 oral squamous carcinoma cells.</div></div><div><h3>Results</h3><div>PPT exerted pronounced inhibitory effects on cell proliferation and anchorage-independent growth accompanied by morphological indications of MC, such as enlarged multinucleated cells. DNA damage induced by PPT resulted in ataxia telangiectasia mutated kinase and checkpoint kinase 2 activation, leading to G<sub>2</sub>/M arrest and cyclin B1upregulation. Importantly, PPT-induced MC was followed by apoptosis, as evidenced by an increased sub-G<sub>1</sub> population, Annexin V positivity, and caspase activation. Mitochondrial dysfunction, as indicated by altered membrane potential and enhanced Bax expression, underscored the apoptotic process. Caspase-2 activation emerged as a pivotal event, cleaving Bid and establishing a link between MC and the intrinsic apoptotic pathway. The effects were consistent across both 2D and 3D models, suggesting a robust therapeutic potential.</div></div><div><h3>Conclusions</h3><div>This study provides compelling evidence supporting the potential therapeutic significance of inducing MC-mediated apoptosis in OSCC. The results underscore the role of PPT and its derivatives, such as etoposide and teniposide, in targeting rapidly dividing cancer cells through interference with mitotic progression, offering insights into novel therapeutic strategies for oral cancer.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106396"},"PeriodicalIF":2.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Protective effect of a novel antioxidant gel containing resveratrol and sodium fluoride on dentin erosion in the presence of acquired salivary pellicle: An in vitro study 含有白藜芦醇和氟化钠的新型抗氧化凝胶在获得性唾液膜存在下对牙本质侵蚀的保护作用:体外研究。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-15 DOI: 10.1016/j.archoralbio.2025.106395
Loraine Perez Manzoli , Luan Júlio Ruiz da Silva , George Clay dos Santos Caracas , Kalinca Furtado de Oliveira , Walessa Alana Braganca Aragão , Rafael Rodrigues Lima , Milton Carlos Kuga , Cristiane de Melo Alencar
{"title":"Protective effect of a novel antioxidant gel containing resveratrol and sodium fluoride on dentin erosion in the presence of acquired salivary pellicle: An in vitro study","authors":"Loraine Perez Manzoli ,&nbsp;Luan Júlio Ruiz da Silva ,&nbsp;George Clay dos Santos Caracas ,&nbsp;Kalinca Furtado de Oliveira ,&nbsp;Walessa Alana Braganca Aragão ,&nbsp;Rafael Rodrigues Lima ,&nbsp;Milton Carlos Kuga ,&nbsp;Cristiane de Melo Alencar","doi":"10.1016/j.archoralbio.2025.106395","DOIUrl":"10.1016/j.archoralbio.2025.106395","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the protective effect of experimental gels containing resveratrol, with or without 1.23 % sodium fluoride (NaF), and their interaction with the salivary pellicle on dentin erosion.</div></div><div><h3>Design</h3><div>Gels with 5 % or 10 % resveratrol (w/w) were prepared, with NaF added at 1.23 % (w/w). Total polyphenol content (TPC) and antioxidant capacity (ORAC) were determined. Human dentin specimens (n = 210) were allocated into seven groups (n = 30): negative control, NaF (1.23 %), 5 % resveratrol, 10 % resveratrol, 5 % resveratrol + NaF, 10 % resveratrol + NaF, and positive control (commercial anti-erosion toothpaste). Each group was divided according to the presence or absence of salivary pellicle (n = 15). After gel application, specimens were submitted to a 7-day erosive–abrasive cycling protocol. Outcomes included surface loss (3D CLM), collagen degradation after collagenase, calcium release (atomic absorption spectrometry), and tubule obliteration (CLM). Data were analyzed using two-way ANOVA and Tukey’s test (α = 0.05).</div></div><div><h3>Results</h3><div>The 10 % resveratrol gel had the highest TPC and ORAC (p = 0.03), followed by 10 % resveratrol + NaF (p = 0.013), whereas 5 % resveratrol showed lower values (p = 0.021). The salivary pellicle significantly reduced dSL-eroded, dColl, and CaR in all groups (p = 0.001). Combined formulations showed the greatest protection: G5 and G6 had lower dSL-eroded (p = 0.002, 0.004), dColl (p = 0.014, 0.021), and dSL-total (p = 0.007, 0.008), with G6 showing the lowest CaR (p = 0.023).</div></div><div><h3>Conclusion</h3><div>The combination of 10 % resveratrol and NaF effectively protected against dentin erosion, with salivary pellicle enhancing these effects.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106395"},"PeriodicalIF":2.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Endocannabinoid system in periodontitis: A systematic review and in silico analyses 内源性大麻素系统在牙周炎:系统回顾和计算机分析。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-13 DOI: 10.1016/j.archoralbio.2025.106394
Lélio Fernando Ferreira Soares , Jovânia Alves Oliveira , Andressa Vilas Boas Nogueira , Carlos Rossa Júnior , Suzane Cristina Pigossi , James Deschner , Joni Augusto Cirelli
{"title":"Endocannabinoid system in periodontitis: A systematic review and in silico analyses","authors":"Lélio Fernando Ferreira Soares ,&nbsp;Jovânia Alves Oliveira ,&nbsp;Andressa Vilas Boas Nogueira ,&nbsp;Carlos Rossa Júnior ,&nbsp;Suzane Cristina Pigossi ,&nbsp;James Deschner ,&nbsp;Joni Augusto Cirelli","doi":"10.1016/j.archoralbio.2025.106394","DOIUrl":"10.1016/j.archoralbio.2025.106394","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review with in silico investigation discusses the involvement of the endocannabinoid system (ECS), particularly CB1 and CB2 receptors (genes <em>CNR1</em> and <em>CNR2</em> respectively), in periodontal health and disease (PD).</div></div><div><h3>Methods</h3><div>PubMed/MEDLINE, Embase, Web of Science, Scopus, and The Cochrane Library databases were searched for studies on periodontitis and ECS published up to August 2024. The GSE16134 dataset was used for analyses of differential gene expression, correlation of ECS genes, evaluation of biomarkers and functional enrichment analysis.</div></div><div><h3>Results</h3><div>Nine studies met the inclusion criteria (three clinical and six preclinical studies). Clinical studies demonstrated that <em>CNR2</em> gene expression was significantly reduced in periodontitis, while <em>CNR1</em> showed minor changes. Animal studies with CB2 activation by different therapies increased receptor expression, reduced pro-inflammatory cytokines, and mitigated alveolar bone loss. CB1 activation also reduced inflammation and bone loss. Anandamide (AEA), an endogenous ligand of the cannabinoid receptors, exhibited anti-inflammatory effects, with endogenous levels decreasing after therapy. Bioinformatics analysis revealed that <em>CNR1</em> expression in PD tissues was positively associated with genes involved in B-cell activation and humoral immune responses. In contrast, <em>CNR2</em> expression showed strong correlations with genes related to immune regulation and extracellular matrix remodeling, suggesting distinct yet complementary roles for CB1 and CB2 in periodontal inflammation.</div></div><div><h3>Conclusions</h3><div>The ECS participates in periodontal inflammation, with CB2 activation emerging as a promising therapeutic target.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106394"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparative analysis of antibacterial and antibiofilm potential of chemically- and green laser-produced gold and silver nanoparticles against dental pathogens 化学和绿色激光制备的金、银纳米颗粒对口腔病原体的抗菌和抗生物膜潜能的比较分析。
IF 2.1 4区 医学
Archives of oral biology Pub Date : 2025-09-13 DOI: 10.1016/j.archoralbio.2025.106393
Milica Nemoda , Marija Radovanović , Sanja Živković , Miloš Momčilović , Vladimir Rajić , Marko Bošković , Biljana Nikolić , Jelena Marinković
{"title":"Comparative analysis of antibacterial and antibiofilm potential of chemically- and green laser-produced gold and silver nanoparticles against dental pathogens","authors":"Milica Nemoda ,&nbsp;Marija Radovanović ,&nbsp;Sanja Živković ,&nbsp;Miloš Momčilović ,&nbsp;Vladimir Rajić ,&nbsp;Marko Bošković ,&nbsp;Biljana Nikolić ,&nbsp;Jelena Marinković","doi":"10.1016/j.archoralbio.2025.106393","DOIUrl":"10.1016/j.archoralbio.2025.106393","url":null,"abstract":"<div><h3>Objectives</h3><div>This study compared antibacterial and antibiofilm effectiveness of chemically (chem_) and pulsed laser ablation-synthesized (laser_) gold (Au) and silver (Ag) nanoparticles (NPs), namely chem_AuNPs, chem_AgNPs, laser_AuNPs, and laser_AgNPs, against four <em>Streptococcus</em> species (<em>S. mutans, S. mitis, S. sanguinis,</em> and <em>S. gordonii</em>), essential for cariogenic biofilm development.</div></div><div><h3>Design</h3><div>Nanoparticles were characterized for size and stability using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Their antibacterial and antibiofilm activities were tested in microdilution and crystal violet assays, respectively. <em>Ex vivo</em> evaluation of the nanoparticles antibiofilm activity was performed against a four-species biofilm on enamel discs by quantifying CFU. Scanning electron microscopy was used to visualize the reduction in biofilm mass on enamel discs treated with laser_Au and Ag nanoparticles.</div></div><div><h3>Results</h3><div>TEM showed nanoparticle sizes ranging 5.89–18.49 nm. DLS revealed the stability of NPs colloid solutions, with ζ-potential approximately −30 mV over 21 days. All NPs exhibited strong antibacterial activity, with MIC values of 56.9–120.6 μg mL-1 for chem_AuNPs and 1.9–30.8 μg mL-1 for all the rest. The evaluation of NPs potential in biofilm prevention showed the following trend in activity: laser_AuNPs&gt;laser_AgNPs&gt;chem_AuNPs&gt;chem_AgNPs. AuNPs, regardless of synthesis method, were as effective as the positive control (chlorhexidine-mouthwash) against the multispecies biofilm settled on enamel discs, while AgNPs were nearly twice as efficient.</div></div><div><h3>Conclusions</h3><div>AuNPs and AgNPs could be alternatives for managing cariogenic bacteria both planktonic and within biofilms. Laser_AgNPs, being more efficient than commercial mouthwash, seem to be the most valuable candidate.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106393"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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