Archives of oral biology最新文献

{"title":"Characteristics of oral microbiomics with soldiers in the army before and after high-intensity physical training","authors":"Yutong Zhou ,&nbsp;Congsheng Wen ,&nbsp;Qixia Zhang ,&nbsp;Ziteng Gu ,&nbsp;Luya Lian ,&nbsp;Kexin Xue ,&nbsp;Tianyi Xu ,&nbsp;Zhehao Lin ,&nbsp;Weiguo Wang ,&nbsp;Haihua Zhu","doi":"10.1016/j.archoralbio.2025.106347","DOIUrl":"10.1016/j.archoralbio.2025.106347","url":null,"abstract":"<div><h3>Objectives</h3><div>This paper aims to investigate the changes in soldiers' oral microbiome and metabolic levels after a month of high-intensity training.</div></div><div><h3>Design</h3><div>We collected saliva samples from 10 soldiers with good oral health and hygiene habits before and after training. Subsequently, DNA extraction, metagenomic sequencing, and phylogenetic analysis of the oral microbiome were conducted.</div></div><div><h3>Results</h3><div>7733 bacterial species from 113 known bacterial phyla and 2017 genera detected in 20 samples. The diversity and richness of saliva microorganisms before and after training were similar <em>(p &gt; 0.05)</em>, while beta diversity analysis showed structural differences in microbiota at the phylum and genus levels <em>(p &lt; 0.05)</em>. The relative abundance of 27 genera such as <em>Proteobacteria</em>, <em>Neisseria</em>, <em>Morococcus cerebrosus</em> and <em>Eikenella</em> in soldiers' saliva significantly increased after high-intensity training <em>(p &lt; 0.05)</em>. Conversely, the relative abundance of 20 genera such as <em>Bacteroidota</em>, <em>Veillonella</em>, <em>Parvimonas micra</em>, <em>Prevotella oris</em>, <em>Peptostreptococcus</em>, and <em>Treponema</em> decreased <em>(p &lt; 0.05)</em>. At the metabolic level, training resulted in a relative increase <em>(p &lt; 0.05)</em> in various pathways, including amino acid metabolism, sulfur metabolism, glutathione metabolism, and Tyrosine metabolism. By comparison, after training, carbohydrate metabolism, glycan biosynthesis, metabolism, the HIF-1 signaling pathway, and necroptosis revealed a relative decrease <em>(p &lt; 0.05).</em></div></div><div><h3>Conclusions</h3><div>This paper reveals the changes in the saliva microbiome of soldiers after one month of high-intensity training, in which the relative abundance of biomarkers of periodontal disease, caries, and other oral diseases represented by <em>peptostreptococcus</em>, <em>prevotella oris</em>, <em>treponema</em>, etc., are significantly reduced, suggesting that long-term high-intensity training may have a positive effect on oral health.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106347"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144588906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"IRF6 regulates adherens junction proteins and inflammatory cytokines in salivary acinar cells and its expression is elevated in Sjӧgren’s syndrome","authors":"Vi Pham ,&nbsp;Antonio Hernandes Chaves Neto ,&nbsp;Julia Zheng ,&nbsp;Mary Elmeniawi ,&nbsp;Krishna Kumar Kookal ,&nbsp;Muhammad F. Walji ,&nbsp;Mary C. Farach-Carson ,&nbsp;Walid D. Fakhouri","doi":"10.1016/j.archoralbio.2025.106348","DOIUrl":"10.1016/j.archoralbio.2025.106348","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the mechanism of Interferon Regulatory Factor 6 (IRF6) function in regulating adherens junction proteins and inflammatory cytokines in human salivary acinar cells and Sjögren’s syndrome biopsies.</div></div><div><h3>Design</h3><div>We used mouse model, salivary cell lines, and human salivary gland (SG) biopsies to investigate IRF6 function in SG cellular integrity and immunomodulation.</div></div><div><h3>Results</h3><div>In <em>Irf6</em>-null mice, we observed myoepithelial cell detachment from acinar cells of major SGs, and the acini were remarkably disorganized compared to wild-type littermates. At the molecular level, the acinar differentiation markers E-cadherin and MIST1 were markedly reduced in <em>Irf6</em>-null embryonic SG, while the proinflammatory cytokines, IL6, IL17A, and IL22, expression was elevated in major SG. In human salivary NS-SV-acinar and adenocarcinoma cells, IRF6 knockdown resulted in a reduction of β-catenin, RAB11b, and the inflammatory cytokines TGFβ3, IL6, IL17A, and IL22, while IRF6 overexpression led to an increase in adherens junction proteins and pro-inflammatory cytokines. Consequently, cell-cell contact and junction of acinar cells were disrupted. Additionally, treatment of acinar cells with TGFβ3 enhanced IRF6 and its target genes expression and rescued the branching morphogenesis during development of <em>Irf6</em>-null SG explants. In Sjögren’s syndrome (SS) biopsies, IRF6 level was remarkably elevated in salivary SS affected acini, along with increased levels of E-cadherin and inflammatory cytokines than control biopsies.</div></div><div><h3>Conclusion</h3><div>IRF6 plays a crucial role in regulating adherens junction proteins and inflammatory cytokines in acinar cells, and increased IRF6 levels in SS biopsies might explain the compromised intercellular space of acinar cells and elevated cytokines in affected SS biopsies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106348"},"PeriodicalIF":2.2,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KEAP1-NRF2-IKKβ crosstalk protects periodontal ligament fibroblasts subjected to mechanical vibratory forces","authors":"Swathi Jayan ,&nbsp;Jeena John ,&nbsp;Madhav Manoj K,&nbsp;Midhun T U,&nbsp;Rahul R S,&nbsp;Deepak D R,&nbsp;Vinod Krishnan","doi":"10.1016/j.archoralbio.2025.106345","DOIUrl":"10.1016/j.archoralbio.2025.106345","url":null,"abstract":"<div><h3>Objectives</h3><div>When cells experience acute trauma from forces, cytoprotective molecules such as NRF2 and activation of inflammatory pathways led by NFkB are synthesized and released. An action-binding protein, KEAP1, plays a central role in these activities. The present study aimed to determine the effect of differential magnitudes of vibratory mechanical force application on the KEAP1 molecule and its downstream regulators, such as NRF2 and IKKβ.</div></div><div><h3>Design</h3><div>A fibroblast cell culture was performed on periodontal ligament cells scraped from atraumatically extracted premolar teeth. These cells were subjected to low (50 Hz), medium, (100 Hz) high (150 Hz), and very high (200 Hz) vibratory settings for 20 min at 0, 24, and 48 h. mRNA and protein analysis of KEAP1, NRF2, and IKKβ was performed using real-time PCR, indirect ELISA test, and Western blot, respectively.</div></div><div><h3>Results</h3><div>The results indicated that vibratory mechanical force application interfered with cultured periodontal ligament fibroblasts' normal growth and homeostasis. While KEAP1's mRNA expression and protein activity were downregulated, NRF2's and IKKβ's were upregulated. As force magnitude increases, KEAP1's downregulation increases, while NRF2 and IKKβ's upregulation continues.</div></div><div><h3>Conclusions</h3><div>The study revealed an inverse relationship between KEAP1, NRF2, and IKKβ in periodontal ligament fibroblasts subjected to mechanical forces. A decrease in KEAP1 and an increase in NRF2 and IKKβ mRNA and protein levels to different vibrational frequencies can evoke cytoprotective responses in the cells.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106345"},"PeriodicalIF":2.2,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144579434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioinformatics-driven salivary microbial and functional profiling for identifying biomarkers in oral cancer and tobacco abusers in the Indian population","authors":"Chakresh Kumar Jain ,&nbsp;Sarita Maurya ,&nbsp;Ankita Singh","doi":"10.1016/j.archoralbio.2025.106346","DOIUrl":"10.1016/j.archoralbio.2025.106346","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate alterations in the salivary microbiome and its functional potential in Oral Squamous Cell Carcinoma (OSCC) patients, tobacco abusers, and healthy individuals in order to identify microbial biomarkers and gain insight into OSCC pathogenesis.</div></div><div><h3>Design</h3><div>Saliva-derived 16S rRNA gene sequences from OSCC patients, tobacco users, and healthy controls were obtained from the Indian Biological Data Centre (IBDC). Taxonomic classification was performed using QIIME2, and functional prediction was conducted with MicFunPred. Alpha and beta diversity, differential abundance, and pathway enrichment analyses were used to compare microbial communities and functions among the groups.</div></div><div><h3>Results</h3><div>OSCC samples showed significantly reduced alpha diversity and distinct microbial community profiles relative to healthy controls. Genera including <em>Porphyromonas</em>, <em>Streptococcus</em>, <em>Rothia</em>, and <em>Parvimonas</em> were enriched in OSCC and are associated with inflammation and carcinogenesis. Tobacco users exhibited increased microbial richness, with dominance of <em>Prevotella</em>, <em>Veillonella</em>, and <em>Peptostreptococcus</em>, indicative of adaptation to chemical exposure. Functional predictions in OSCC samples indicated enrichment in pathways related to bacterial chemotaxis, lipopolysaccharide biosynthesis, glycolysis, and DNA repair. Tobacco-associated microbiota showed elevated oxidative stress response and detoxification pathways.</div></div><div><h3>Conclusions</h3><div>Microbial dysbiosis and functional alterations in the oral microbiome are strongly associated with OSCC and tobacco use. The identification of disease-specific microbial signatures and pathways highlights the potential of the oral microbiome as a non-invasive biomarker and therapeutic target for precision medicine in OSCC, particularly in the Indian population.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106346"},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COL1-related overlap disorder: An emerging phenotype linked to mono- and bi-allelic COL1A1/2 variants","authors":"Nehal F. Hassib ,&nbsp;Rasha M. Elhossini ,&nbsp;Inas S.M. Sayed ,&nbsp;Mona S. Aglan ,&nbsp;Mohamed S. Abdel-Hamid","doi":"10.1016/j.archoralbio.2025.106344","DOIUrl":"10.1016/j.archoralbio.2025.106344","url":null,"abstract":"<div><h3>Objectives</h3><div>We describe the clinical, radiological, and oro-dental findings of four unrelated families with unusual skeletal phenotypes caused by mono- and bi- allelic <em>COL1A1/2</em> variants.</div></div><div><h3>Design</h3><div>The study included five Arab patients with clinical manifestations resembling osteogenesis imperfecta in the presence of variable degrees of osteoporosis, bone deformities, gray sclera, Wormian bones in the skull, and oro-dental abnormalities. However, bone fractures, which are considered the cardinal feature in osteogenesis imperfecta patients, were not documented in any of the cases. In addition, they also lacked the characteristic features of Ehlers-Danlos syndrome. Exome sequence was used to identify the causative variants.</div></div><div><h3>Results</h3><div>A new homozygous missense variant in <em>COL1A1</em>: c.4340 T &gt; G; p.(Val1447Gly) was identified in one family, while the remaining three families harbored two recurrent and one novel heterozygous variants in <em>COL1A2</em>: [c.1171 G&gt;A; p.(Gly391Ser) and c.1253 G&gt;C; p.(Gly418Ala)] and <em>COL1A1</em>: c.1678 G&gt;A; p.(Gly560Ser), respectively.</div></div><div><h3>Conclusions</h3><div>We present a new patient harboring a homozygous <em>COL1A1</em> variant with a distinctive skeletal phenotype in comparison to the few previously reported patients in the literature. In addition, we describe three families with osteogenesis imperfecta like phenotype harboring monoallelic <em>COL1A1/2</em> variants, expanding the spectrum of COL1-related overlap disorder.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106344"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory, antioxidant, and antimicrobial evaluation of morin","authors":"Luciana Solera Sales ,&nbsp;Benjamin Hewitt ,&nbsp;Maria Muchova ,&nbsp;Fernanda Lourenção Brighenti ,&nbsp;Sarah A. Kuehne ,&nbsp;Melissa M. Grant ,&nbsp;Michael R. Milward","doi":"10.1016/j.archoralbio.2025.106343","DOIUrl":"10.1016/j.archoralbio.2025.106343","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the anti-inflammatory and antioxidant activity of morin in an epithelial cell culture inflammatory model, as well as its antimicrobial activity against periodontal pathogens. Effects of morin alone or slow-release from a polymer-based formulation were compared.</div></div><div><h3>Methods</h3><div>An oral epithelial cell line (H400) was pre-conditioned with 0.125 mg/mL morin (free morin or morin in the formulation) for 8 h. The cells were challenged with heat-killed <em>Fusobacterium nucleatum</em> and <em>Porphyromonas gingivalis</em> (MOI 100:1) for 24 h. The production of GM-CSF was measured by enzyme-linked immunosorbent assay (ELISA) and the gene expression levels of IL-8, GM-CSF, IL1B, NF-kB and NLRP3 were evaluated by RT – PCR. Neutrophils were pre-conditioned with 0.01 mg/mL morin and stimulated immediately with heat-killed <em>F. nucleatum</em> or <em>P. gingivalis</em> (MOI 100:1) for 2.5 h and then total reactive-oxygen-species (ROS) levels were determined using enhanced chemiluminescence. <em>F. nucleatum</em> and <em>P. gingivalis</em> as well as a multi-species biofilm (<em>Streptococcus oralis</em>, <em>F. nucleatum</em> and <em>P. gingivalis</em>) were exposed to 1 mg/mL morin for 24 h, planktonic culture and for 7 days, multi-species biofilm. After exposure, the microbial viability (CFU/mL) and biofilm biomass (crystal violet assay) were determined. Live/dead staining and confocal laser scanning microscopy were also performed. Data were submitted to one way ANOVA followed by Tukey's post-hoc test (α=0.05).</div></div><div><h3>Results</h3><div>Morin and the polymer-based formulation containing morin significantly attenuated secretion of GM-CSF, significantly reduced ROS generation and the gene expression levels of the cytokines tested (p &lt; 0.05). Microbial viability and the biofilm biomass significantly reduced (p &lt; 0.05) after treatment with morin and the polymer-based formulation containing morin.</div></div><div><h3>Conclusions</h3><div>Morin showed an anti-inflammatory and antioxidant effect as well as antimicrobial properties against periodontal pathogens. In addition, polymer-based formulation enhanced these effects.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106343"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and morphological analysis of permeable dentin exposed to experimental solutions containing epigallocatechin-3-gallate encapsulated in chitosan nanoparticles","authors":"Davi Ariel Nobuo Bepu ,&nbsp;Karen Pintado-Palomino ,&nbsp;Letícia de Sousa Franco ,&nbsp;Débora Fernandes Costa Guedes ,&nbsp;Luiza Araújo Gusmão ,&nbsp;Antônio Cláudio Tedesco ,&nbsp;Silmara Aparecida Milori Corona","doi":"10.1016/j.archoralbio.2025.106342","DOIUrl":"10.1016/j.archoralbio.2025.106342","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of epigallocatechin 3-gallate (EGCG) and chitosan-based solutions on the chemical and morphological structure of permeable dentin using Fourier Transform Infrared Spectroscopy with Attenuated Reflectance Accessory (FTIR-ATR), Energy dispersive X-ray spectroscopy – EDS, and Scanning Electron Microscopy (SEM) analyses.</div></div><div><h3>Design</h3><div>Bovine root dentin specimens were demineralized with EDTA to simulate dentin hypersensitivity. Specimens were divided into six groups (n = 6): EGCG encapsulated in chitosan nanoparticles (Nchi + EGCG), chitosan nanoparticles (Nchi), EGCG, Elmex Sensitive, deionized water (negative control), and No solution/no acid challenge. Solutions were applied, followed by cycles of citric acid (2 min, 0.3 %, pH 2.45) and artificial saliva (1 h, 4 ×/7 days). Data were analyzed using Kolmogorov-Smirnov, ANOVA, and Tukey’s tests.</div></div><div><h3>Results</h3><div>FTIR spectra revealed enhanced phosphate, carbonate, and amide bands in Nchi + EGCG, EGCG, Nchi, and Elmex groups. EDS showed no significant differences in Ca/P ratios among treated groups, but Nchi and Nchi + EGCG differed significantly from the No solution/no acid challenge (p &lt; 0.05). There were significant differences in the number of dentinal tubules between the Nchi/Nchi + EGCG and EGCG/No solution applied groups (P &lt; 0.05), but no differences among experimental solutions and negative control (p &gt; 0.05). Nchi + EGCG and EGCG showed the smallest open dentinal tubule areas (P = 0.00 and P = 0.001, respectively).</div></div><div><h3>Conclusion</h3><div>Nchi + EGCG modified dentin by enhancing collagen interaction; however, it does not alter Ca/P ratio. Solutions containing EGCG (EGCG and Nchi + EGCG) can reduce the open tubule area.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106342"},"PeriodicalIF":2.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of mechanical load on mandibular condylar cartilage and subchondral bone of male rats","authors":"Takahiro Honda,&nbsp;Tomohiro Fukunaga,&nbsp;Arata Ito,&nbsp;Michiko Yoshida,&nbsp;Kozue Yasuno,&nbsp;Hiroka Tsumaki,&nbsp;Kaya Yamaguchi,&nbsp;Yoon Seung Choi,&nbsp;Itaru Mizoguchi","doi":"10.1016/j.archoralbio.2025.106341","DOIUrl":"10.1016/j.archoralbio.2025.106341","url":null,"abstract":"<div><h3>Objective</h3><div>The mandibular condylar cartilage plays significant roles in growth and articular function. This study evaluated the influence of increased joint loading on the growth of the condylar cartilage and the morphology of the mandible.</div></div><div><h3>Design</h3><div>Forty 7-week-old male Wistar rats were assigned to two groups: a control group and an incisal bite plane group. The condylar cartilage and subchondral bone were evaluated via histochemistry, bone histomorphometry with microcomputed tomography (micro-CT), and real-time polymerase chain reaction (PCR) at 2 and 4 weeks. The morphology of the mandible was analyzed via micro-CT.</div></div><div><h3>Results</h3><div>The chondrocytic cell layer became thinner in the posterosuperior region after 2 and 4 weeks of wearing the bite plane. Hypertrophied chondrocytes and calcified cartilage matrix disappeared. Subchondral bone, which runs parallel to the lower border of the chondrocytic cell layer, increased. Bone volume fraction (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N) increased, while the amount of endochondral bone formation decreased, in the posterosuperior region of the condyle in the bite plane group, which also had reduced mRNA levels of type II collagen, type X collagen, and tartrate-resistant acid phosphatase but increased levels of osteocalcin and alkaline phosphatase. The total mandibular length and ramus height were significantly shorter in the bite plane group, although there was no significant group difference in the length of the mandibular body.</div></div><div><h3>Conclusions</h3><div>The biomechanical environment regulates the cellular organization of the condylar cartilage and bone formation in its subchondral region, which may eventually change mandibular morphology.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106341"},"PeriodicalIF":2.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dentinogenesis imperfecta, sex, and tooth type on the compositional and structural organization of the dentin-enamel junction in the osteogenesis imperfecta murine model","authors":"Sobhan Katebifar ,&nbsp;Kai Clarke ,&nbsp;Bradley S. Rosenberg ,&nbsp;Michael Truhlar ,&nbsp;Alix C. Deymier","doi":"10.1016/j.archoralbio.2025.106340","DOIUrl":"10.1016/j.archoralbio.2025.106340","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigates the structural and compositional features of the dentin-enamel junction in the osteogenesis imperfecta murine teeth, focusing on the effects of dentinogenesis imperfecta, sex, and tooth type.</div></div><div><h3>Design</h3><div>The study compares dentin-enamel junctions in molars and incisors, as these teeth experience different functions and growth patterns, using a multiscale approach that combines micro-computed tomography (micro-CT), Scanning Electron Microscopy (SEM), and Raman Spectroscopy techniques.</div></div><div><h3>Results</h3><div>The width of the mantle dentin is significantly reduced in dentinogenesis imperfecta-affected mice. Female mice show significantly larger gradients in mineral content and structural dentin-enamel junction than male mice. Molars have larger gradient widths compared to incisors. Molars exhibit higher dentin porosity and a smaller mantle dentin width compared to incisors.</div></div><div><h3>Conclusion</h3><div>Dentinogenesis imperfecta causes a decrease in tubule diameter and an increase in the number of tubules, which may contribute to heightened tooth sensitivity in dentinogenesis imperfecta-affected mice. Females may have better resilience to stress and fractures, potentially explaining why dentinogenesis imperfecta might be diagnosed more frequently in males. Larger gradient widths in molars may help protect them due to their different functions and mechanical stresses.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106340"},"PeriodicalIF":2.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TGF-β1 regulates pannexin1 channels and evokes apoptotic response in osteoblasts","authors":"Wenjing Liu","doi":"10.1016/j.archoralbio.2025.106336","DOIUrl":"10.1016/j.archoralbio.2025.106336","url":null,"abstract":"<div><h3>Objective</h3><div>Extracellular ATP is suggested to be involved in cell-cell interactions and TGF-β1 stimulates ATP release through pannexin1 channels. However, the role of TGF-β1 in regulating of pannexin1 channels and cell apoptosis remains unclear. In the present study, the aim was to clarify the role of TGF-β1 in relation to pannexin1 channels and cell apoptosis in osteoblasts.</div></div><div><h3>Design</h3><div>The detection of pannexin1 expression induced by TGF-β1 was achieved using immunofluorescence labeling and western blot analysis. The activity of pannexin1 channels was detected by the dye uptake assay. This study employed the MEK inhibitor U0126 to block ERK signaling in order to investigate the signaling pathway which is involved in the effect of TGF-β1 on pannexin1. In order to determine the expression of cleaved caspase-3 in osteoblasts, immunofluorescence labeling was employed. Flow cytometry was performed to detect the rate of apoptotic cells.</div></div><div><h3>Results</h3><div>Initially, the data of this study showed that TGF-β1 increase the expression of pannexin1 in both primary osteoblasts and the MC3T3 cell line. This study also confirmed that TGF-β1 triggers osteoblast ethidium bromide (EtBr) dye uptake by pannexin1 channels. The inhibition of ERK signaling pathway eliminated TGF-β1’s ability to promote pannexin1. Pannexin 1 is up-regulated in osteoblasts that undergo apoptosis due to high concentration of TGF-β1.</div></div><div><h3>Conclusions</h3><div>According to these findings, high concentration of TGF-β1 up-regulates the expression of pannexin1 and the activation of pannexin1 channels. The ERK signaling pathway mediates this regulation, which induces the apoptosis of osteoblasts.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106336"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}