Archives of oral biology最新文献

{"title":"Gallic acid promotes M2 macrophage polarization through mitochondrial oxidative phosphorylation in periodontitis","authors":"Ruobing Zhang ,&nbsp;Wenjing Yang ,&nbsp;Kai Li ,&nbsp;Xiaolong Zhang ,&nbsp;Jie Liu ,&nbsp;Lin Ai","doi":"10.1016/j.archoralbio.2025.106237","DOIUrl":"10.1016/j.archoralbio.2025.106237","url":null,"abstract":"<div><h3>Objective</h3><div>This in vitro investigation assessed the role of gallic acid in regulating macrophage polarization.</div></div><div><h3>Design</h3><div>In this study, RAW264.7 macrophages were treated with <em>P. gingivalis</em> lipopolysaccharide (LPS) to mimic inflammatory conditions in periodontitis. Immunofluorescence staining, enzyme‑linked immunosorbent (ELISA) assay, RNA sequencing, and seahorse metabolic profile assay were used to assess the effects of gallic acid on macrophage polarization, cytokine release, underlying mechanism and metabolic profile.</div></div><div><h3>Results</h3><div>The study demonstrated that treatment with gallic acid could induce M2 polarization of macrophages (<em>P</em> &lt; 0.01) and increase the production of anti-inflammatory cytokines (<em>P</em> &lt; 0.01). Analysis of RNA sequencing data showed enrichment of mitochondrial oxidative phosphorylation, regulation of metabolic processes, and the PI3K-Akt signaling pathway in LPS-treated macrophages treated with gallic acid. Furthermore, gallic acid was found to enhance mitochondrial oxidative phosphorylation activity through the PI3K-Akt signaling pathway in RAW264.7 cells.</div></div><div><h3>Conclusions</h3><div>Gallic acid treatment has the potential to promote M2 polarization of macrophages by modulating mitochondrial oxidative phosphorylation via the PI3K-Akt signaling pathway.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106237"},"PeriodicalIF":2.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single monoiodoacetic acid injection reveals toll-like receptor, oestrogen, oxidative stress, and altered energy metabolism as key drivers of temporomandibular joint osteoarthritis in female rats","authors":"Shasha Liu ,&nbsp;Chaolun Wu ,&nbsp;Yuan Yao ,&nbsp;Xiongwei Dong ,&nbsp;Lili Xu","doi":"10.1016/j.archoralbio.2025.106233","DOIUrl":"10.1016/j.archoralbio.2025.106233","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to establish a reproducible and minimally invasive rat model of temporomandibular joint osteoarthritis (TMJ-OA) using intra-articular monoiodoacetic acid (MIA) injection, and to investigate the pathological mechanisms underlying TMJ-OA development, aimed at providing insights for potential clinical treatments.</div></div><div><h3>Design</h3><div>We compared the effects of single versus multiple MIA injections on body weight, pain behaviour, and condylar pathology in female Sprague–Dawley rats. We longitudinally assessed the progression of TMJ-OA over 5 weeks by evaluating condylar pathology and immunohistochemical staining. We investigated the potential mechanism of MIA-induced TMJ-OA through transcriptome sequencing and polymerase chain reaction validation.</div></div><div><h3>Results</h3><div>A single MIA injection (0.5 mg) into the joint space effectively induced TMJ-OA in rats and sustained inflammatory reactions and pain without significantly affecting weight. MIA continuously promoted the development of TMJ-OA through the activation of the toll-like receptor pathway, oestrogen metabolism promotion, oxidative stress response enhancement, and energy metabolism alteration in condylar chondrocytes.</div></div><div><h3>Conclusion</h3><div>We have presented a simple and minimally invasive method for modelling TMJ-OA in rats, which can be utilised in animal trials focusing on TMJ-OA treatment strategies. The study also reveals toll-like receptor, oestrogen, oxidative stress, and altered energy metabolism as key drivers of TMJ-OA in female rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106233"},"PeriodicalIF":2.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of oral conditions to explore subsistence strategies in the ecologically diverse environments of the Canary Islands during the Amazigh period (1st to 15th centuries CE)","authors":"Aarón Morquecho Izquier ,&nbsp;Rebeca García González ,&nbsp;Elías Sánchez Cañadillas ,&nbsp;Jonathan Santana","doi":"10.1016/j.archoralbio.2025.106236","DOIUrl":"10.1016/j.archoralbio.2025.106236","url":null,"abstract":"<div><div>Objective:</div><div>This study examines oral health conditions in the pre-European Amazigh population of the Canary Islands (1st–15th centuries) to uncover patterns linked to island-specific environments and subsistence practices. The Canary Islands offered diverse ecological settings that shaped how the Amazigh adapted their agricultural practices, creating landscapes to support settlement.</div><div>Design:</div><div>By analyzing the oral health of 615 adult individuals across seven major islands—including caries, dental wear, calculus, periodontal disease, and antemortem tooth loss—the study identifies three distinct patterns of oral health within the archipelago.</div><div>Results:</div><div>Gran Canaria exhibited the highest prevalence of dental caries, aligning with an agriculture-centered lifestyle. In contrast, La Gomera, Fuerteventura, and Lanzarote showed the lowest caries rates, suggesting less agricultural dependence. El Hierro and La Palma populations presented both high caries prevalence and extensive dental wear, likely due to a diet including famine-related foods like fern roots. The findings also indicate sex-based differences in oral health across islands but no significant distinctions by age.</div><div>Conclusions:</div><div>These patterns highlight a relationship between the diverse biogeography of the islands, subsistence strategies, and oral health in the Amazigh period. This study contributes valuable insights into how different environments and resource availability impacted the diet and health of the Canary Islands’ Amazigh populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106236"},"PeriodicalIF":2.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic polymorphisms on temporomandibular disorders: Network meta-analysis","authors":"Daniel Augusto de Faria Almeida ,&nbsp;Camila Freire Brant ,&nbsp;Letícia da Costa Siqueira ,&nbsp;Lélio Fernando Ferreira Soares ,&nbsp;Jovânia Alves Oliveira ,&nbsp;Daniela Silva Barroso de Oliveira ,&nbsp;Suzane Cristina Pigossi ,&nbsp;Carlos José Soares","doi":"10.1016/j.archoralbio.2025.106235","DOIUrl":"10.1016/j.archoralbio.2025.106235","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this systematic review and network meta-analysis (NMA) is to compare and rank the effects of different genetic polymorphisms on the susceptibility of temporomandibular disorders (TMDs) occurrence.</div></div><div><h3>Design</h3><div>The central question formulated was: \"Are genetic polymorphisms involved in the etiology of TMDs?\" Following PROSPERO registration (CRD42024507886), electronic searches were conducted in five databases for publications up to November 2024.</div></div><div><h3>Results</h3><div>Sixty-three studies were included in the systematic review and 7 composed the NMA. The qualitative analysis summarized the association between 120 genes (and 206 polymorphisms) and TMDs. Thirty-two polymorphisms (in 24 genes) were linked to overall TMDs, while 22 polymorphisms (in 22 genes) with degenerative bone changes in the temporomandibular joint (TMJ). Additionally, 17 polymorphisms were identified in cases of painful chronic TMD, while 12 polymorphisms in intra-articular disorders. These polymorphisms were in genes related to neurotransmission (COMT, ADRB2, DRD2, ANKK1, SLC6A4 and HTR2A), inflammatory mediators (TNFα, IL10 and MMP1), sex hormones (ESR1and ESRRB), oxidative stress (GSTM1) and bone metabolism (VDR). A protective effect for myalgia occurrence with the COMT_rs165774 polymorphism compared to the wild-type genotype was found in the pairwise meta-analysis (AG genotype: OR: 0.33; 95 %CI: 0.14, 0.76; p &lt; 0.01 and GG genotype: OR: 0.32; 95 %CI: 0.14, 0.74; p &lt; 0.01) and this polymorphism showed the highest probability of being associated with the myalgia (97 %) and arthralgia (93 %) conditions.</div></div><div><h3>Conclusions</h3><div>Genetic polymorphisms in genes related to neurotransmission, inflammatory response, and sex hormones seem to be risk factors related to the TMDs pathogenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106235"},"PeriodicalIF":2.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear stress preconditioning enhances periodontal ligament stem cell survival","authors":"Ravipha Suwittayarak ,&nbsp;Nuttha Klincumhom ,&nbsp;Chaloemrit Phrueksotsai ,&nbsp;Nuttapol Limjeerajarus ,&nbsp;Chalida Nakalekha Limjeerajarus ,&nbsp;Hiroshi Egusa ,&nbsp;Thanaphum Osathanon","doi":"10.1016/j.archoralbio.2025.106232","DOIUrl":"10.1016/j.archoralbio.2025.106232","url":null,"abstract":"<div><h3>Objective</h3><div>The study investigated <em>in vitro</em> the influences of shear stress preconditioning on human periodontal ligament stem cells (hPDLSCs) under serum deprivation.</div></div><div><h3>Design</h3><div>hPDLSCs were subjected to shear stress at 0.5 and 5 dyn/cm², both with and without serum starvation. Cell viability and apoptosis were assessed using the Resazurin assay and flow cytometry analysis, respectively. Gene and protein expressions were analysed by real-time polymerase chain reaction, immunofluorescent staining, and Western blotting.</div></div><div><h3>Results</h3><div>Our results revealed that shear stress potentially mitigated serum derivation-induced cell death by inducing cell viability, enhancing colony formation, and inhibiting cell apoptosis. The addition of an ERK inhibitor inhibited the shear stress-induced cell apoptosis resistance. Shear stress treatment upregulated cell viability-related gene expression, including <em>SOX2</em>, <em>SOD1</em> and <em>BIRC5.</em> In particular, shear stress promoted the nuclear translocation of SOX2. Meanwhile, the expression of <em>BIRC5</em> was not inhibited by cycloheximide. Shear stress-induced <em>SOX2</em> and <em>BIRC5</em> expression was attenuated by PI3K and ERK inhibitors, respectively.</div></div><div><h3>Conclusions</h3><div>Shear stress contributes to promoting <em>SOX2</em> and <em>BIRC5</em> expression by hPDLSCs, implicating the promotion of stemness and cell survival under serum starvation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106232"},"PeriodicalIF":2.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between agenesis of permanent teeth and other dental anomalies in nonsyndromic patients: Systematic review","authors":"Paula Cristiana Santos de Aguiar ,&nbsp;Caio Melo Mesquita ,&nbsp;Fabiana Evangelista Lerner ,&nbsp;Laura Bezerra Borges ,&nbsp;Rodrigo Rodrigues ,&nbsp;Walbert A. Vieira ,&nbsp;Luiz Renato Paranhos ,&nbsp;Rui Barbosa de Brito Júnior","doi":"10.1016/j.archoralbio.2025.106223","DOIUrl":"10.1016/j.archoralbio.2025.106223","url":null,"abstract":"<div><h3>Background</h3><div>Most cases of tooth agenesis are presented in the form of hypodontia with different patterns of missing teeth. In addition, there is a frequent clinical association between tooth agenesis and other anomalies.</div></div><div><h3>Objective</h3><div>To systematically analyze the literature on the prevalence of agenesis of permanent teeth associated with other dental anomalies in nonsyndromic patients.</div></div><div><h3>Material and methods</h3><div>An electronic search was performed in seven primary databases (Embase, LILACS, BBO, MedLine <em>via</em> PubMed, SciELO, Scopus, and Web of Science) and two additional ones (OATD, and ProQuest) to partially capture the \"gray literature.\" The PECO strategy was used to identify observational studies evaluating the prevalence of agenesis of permanent teeth associated with other dental anomalies in nonsyndromic patients, without restrictions on publication language or year. Two reviewers extracted the data and assessed the individual risk of bias of the eligible studies using the JBI Critical Appraisal Tools for use in JBI Systematic Reviews - Checklist for Cross-sectional, Case-Control, and Cohort Studies.</div></div><div><h3>Results</h3><div>The electronic search revealed 8659 records, seven of which met the eligibility criteria, and were included in the qualitative synthesis. The studies were published from 1961 to 2024. Patients with agenesis were more likely to present taurodontism, microdontia, enamel hypoplasia and distoangulation of the second premolar. Ectopic eruption, infraocclusion of the first deciduous molar and impacted tooth showed mixed results among the eligible studies.</div></div><div><h3>Conclusion</h3><div>Based on limited evidence, tooth agenesis was positively associated with the prevalence of other concomitant tooth agenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106223"},"PeriodicalIF":2.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA-155 rs767649 polymorphism is associated with susceptibility to peri-implantitis","authors":"Hao Fu ,&nbsp;Xiangrong Li ,&nbsp;Zeneng Xie ,&nbsp;Yinhua Jiang ,&nbsp;Huiling Qin","doi":"10.1016/j.archoralbio.2025.106219","DOIUrl":"10.1016/j.archoralbio.2025.106219","url":null,"abstract":"<div><h3>Objective</h3><div>To examine how the miR-155 rs767649 polymorphism affects miR-155 expression and to investigate its association with peri-implantitis susceptibility.</div></div><div><h3>Design</h3><div>One hundred and eighty-seven peri-implantitis patients and 196 healthy implant subjects were enrolled. The expression level of miR-155 in the subjects' serum was tested using qRT-PCR. The rs767649 polymorphism was genotyped with the TaqMan SNP genotyping assay. The genotype and allele distributions of miR-155 rs767649 polymorphism were compared between the case and control groups. Target genes of miR-155 were predicted using four online databases (ENCORI, miRDB, miRWalk and TargetScan). Functions and pathways of these target genes were annotated through GO and KEGG enrichment.</div></div><div><h3>Results</h3><div>The qRT-PCR results revealed that miR-155 was upregulated in patients with peri-implantitis (<em>P</em> &lt; 0.001) based on Student’s t test analysis. Carriers of the rs767649 TT genotype had higher levels of miR-155 expression in their serum (<em>P</em> &lt; 0.001) and a greater risk of peri-implantitis compared to those with the TA/AA genotype according to the chi-square test results (<em>P</em> = 0.016). The downstream target genes of miR-155 may be involved in pathways such as Kaposi sarcoma-associated herpesvirus infection, ubiquitin-protein hydrolysis, and cell differentiation.</div></div><div><h3>Conclusion</h3><div>The results revealed a correlation between the miR-155 rs767649 polymorphism and susceptibility to peri-implantitis. The rs767649 TT genotype upregulates miR-155 expression, which regulates downstream signaling pathways and increases the chance of peri-implantitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106219"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological evaluation of periapical lesions in developing molars of young male rats","authors":"Mariana Bena Gelio ,&nbsp;Paula Aparecida Nakazone-Guimarães ,&nbsp;Tiago Silva da Fonseca ,&nbsp;Evelin Carine Alves Silva ,&nbsp;Estela Sasso-Cerri ,&nbsp;Paulo Sérgio Cerri","doi":"10.1016/j.archoralbio.2025.106222","DOIUrl":"10.1016/j.archoralbio.2025.106222","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the histopathological features of induced periapical lesions (PL) exposed to the oral environment at different time points in developing molars of rats.</div></div><div><h3>Methods</h3><div>Twenty-four 30-day-old Holtzman rats had the pulp chamber of the mandibular left first molar opened and root canals were exposed to the oral environment. According to the exposure time, the animals were distributed into three groups (n = 8 rats/group): PL induced for 3 days (PLG-3d), PL induced for 1week (PLG-1w) and PL induced for 9 weeks (PLG-9w). The healthy right first molars were used as control group (CG). In paraffin-embedded jaw sections, the number of inflammatory cells (ICs), IL-6-immunolabelled cells, osteoclasts and PLs area were measured. Data were subjected to two-way ANOVA analysis of variance and Tukey's post-test (p &lt; 0.05).</div></div><div><h3>Results</h3><div>The highest values of ICs and IL-6-immunolabelled cells were found in PLG-1w while no significant difference was observed between PLG-3d and PLG-9w specimens. PLG-1w showed a significantly greater number of osteoclasts than PLG-9w, but no significant difference was found in the PL area between these groups.</div></div><div><h3>Conclusions</h3><div>Although the peak of the inflammatory infiltrate, bone resorption and PL size were reached after 1-week, periapical lesions were established after 3 days in developing molars of rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106222"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Botulinum toxin type A inhibits hyperalgesia in the rat masseter muscle in a carrageenan model of myofascial pain","authors":"Ilana Dantas Neves ,&nbsp;Pedro Paulo Pereira Pinho ,&nbsp;Luciana Lyra Casais-e-Silva ,&nbsp;Marcio Cajazeira Aguiar","doi":"10.1016/j.archoralbio.2025.106218","DOIUrl":"10.1016/j.archoralbio.2025.106218","url":null,"abstract":"<div><h3>Objective</h3><div>Previous studies have shown that botulinum toxin type A (BoNT-A) attenuates nociception, but the underlying mechanisms remain unclear. Studies of experimental pain in humans have also shown conflicting results. Carrageenan is commonly used to produce short-term acute inflammation and hyperalgesia in animal models, and the effect of BoNT-A on carrageenan-induced pain in the masseter muscle has not been studied. This study evaluated the antinociceptive and anti-inflammatory effects of intramuscular injection of BoNT-A in an experimental model of inflammatory pain in the masseter muscle of rats.</div></div><div><h3>Design</h3><div>Carrageenan (2 %) was injected into the masseters of sixty rats pretreated with three sessions of BoNT-A (3.5 U/kg) or daily with ibuprofen (40 mg/kg) for seven days. Masseter injected with saline was used as a control. An electronic von Frey anesthesiometer determined the head withdrawal threshold before carrageenan and at 5 h, 1, 3, and 7 days following administration. The masseters were processed for paraffin embedding and H&amp;E staining and subjected to histomorphometric analysis 1 and 8 days after carrageenan administration.</div></div><div><h3>Results</h3><div>Pretreatments with BoNT-A or ibuprofen significantly decreased carrageenan-induced hyperalgesia. BoNT-A did not inhibit inflammation and tissue damage induced by carrageenan.</div></div><div><h3>Conclusions</h3><div>These findings reveal that BoNT-A promotes antinociceptive effects in the masseter muscle during painful conditions independently of anti-inflammatory mechanisms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106218"},"PeriodicalIF":2.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Change and significance of connexin 43 in saliva extracellular vesicles from oral lichen planus patients","authors":"Chun Yao ,&nbsp;Haiyan Xin ,&nbsp;Si Liu ,&nbsp;Hong Wang ,&nbsp;Yanhong Ma ,&nbsp;Chao Yao ,&nbsp;Jian Meng","doi":"10.1016/j.archoralbio.2025.106217","DOIUrl":"10.1016/j.archoralbio.2025.106217","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to explore whether Connexin 43 (Cx43) in salivary extracellular vesicles (EVs) can serve as a biomarker for diagnosing oral lichen planus (OLP), an inflammatory oral mucosal disorder.</div></div><div><h3>Design</h3><div>The study assessed disease activity in OLP patients using the reticulum-erosion-ulcer disease activity score. Saliva EVs were isolated and purified with an EV Enrichment Kit. Transmission electron microscopy was used to examine the morphology and size of EVs, while Western blotting verified EV biomarkers. ELISA measured serum inflammatory factors, such as TNF-α and IL-17. The importance of Cx43 in the diagnosis of OLP was evaluated using receiver operating characteristic (ROC) curve analysis, and correlations were determined through Pearson analysis.</div></div><div><h3>Results</h3><div>The average diameter of isolated saliva EVs was approximately 110 nm, and they expressed high levels of known EV biomarkers. In OLP patients, both Cx43 mRNA and protein levels were significantly higher compared to healthy controls. Furthermore, Cx43 mRNA and protein levels increased gradually with increased disease severity, from the reticular type to the more severe erosion type of OLP. Cx43 mRNA and protein levels were found to effectively diagnosing OLP and correlated significantly with disease activity scores. Moreover, elevated Cx43 mRNA and protein levels showed strong positive correlations with serum TNF-α and IL-17 levels in OLP patients.</div></div><div><h3>Conclusion</h3><div>Cx43 mRNA and protein levels in salivary EVs serve as effective biomarkers for diagnosing OLP and are significantly associated with disease activity and inflammatory markers. This makes Cx43 a promising candidate for non-invasive diagnosis of OLP.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106217"},"PeriodicalIF":2.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}