Archives of oral biology最新文献

{"title":"Fusion of permanent maxillary incisors of archaeological origin: A case report from late medieval Croatia","authors":"Mario Novak ,&nbsp;Igor Erjavec ,&nbsp;Mario Carić ,&nbsp;Željka Bedić ,&nbsp;Veronika Bencerić ,&nbsp;Josip Burmaz ,&nbsp;Dženi Los ,&nbsp;Mislav Čavka ,&nbsp;Marin Vodanović","doi":"10.1016/j.archoralbio.2025.106339","DOIUrl":"10.1016/j.archoralbio.2025.106339","url":null,"abstract":"<div><h3>Objective</h3><div>The study aims to investigate a case of fusion of the permanent central and lateral right maxillary incisors observed in a middle-aged male from the late medieval town of Sisak in Croatia.</div></div><div><h3>Design</h3><div>“Double teeth” is a term for a developmental anomaly caused by congenital, inherited, acquired and/or idiopathic factors resulting in the union of two adjacent teeth. It usually occurs in three different varieties: concrescence, gemination and fusion. Although there are many reports of such disorders in recent populations, similar examples in archaeological contexts are still rare and, in most cases, recorded in primary dentition. We employed a combination of morphological (macroscopic) and state-of-the-art radiographic analysis (micro-CT scanning).</div></div><div><h3>Results</h3><div>The teeth in question are fused in both coronal and radicular pulp parts, indicating a complete fusion in comparison to the left maxillary incisors that have a normal morphology and are completely separated. The fused RI1 and RI2 have a single crown with a slight groove running along the lingual side of the root which is also visible on the buccal side of the root. Two small protrusions (<em>tuberculum dentale</em>) are visible on the lingual side of the crown. Micro-CT scans confirmed that the fused teeth have a single pulp chamber and root canal.</div></div><div><h3>Conclusions</h3><div>The presented case is unique as it is the first known archaeological specimen of permanent maxillary incisors fusion from Europe and one of the very few known cases of the fusion of permanent teeth from archaeological contexts globally.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106339"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix protein composition corresponds to degenerative changes in disc displacement of the temporomandibular joint","authors":"Mattias Ulmner ,&nbsp;Rachael Sugars ,&nbsp;Aron Naimi-Akbar ,&nbsp;Bodil Lund","doi":"10.1016/j.archoralbio.2025.106338","DOIUrl":"10.1016/j.archoralbio.2025.106338","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate changes to the composition of a panel of extracellular matrix (ECM) proteins in the temporomandibular joint (TMJ) posterior disc attachment in relation to the diagnoses disc displacement with/without reduction (DDwR, DDwoR).</div></div><div><h3>Design</h3><div>Patients scheduled for arthroscopy or open joint surgery due to DDwR or DDwoR were eligible for inclusion. During surgery biopsies of the posterior disc attachment were harvested. Proteins were extracted from the biopsies and screened using multiplex bead analysis for 23 different ECM proteins. Patients with DDwR served as controls to DDwoR. The variables gender, age, symptom duration, previous trauma, and Wilkes criteria were assessed in relation to the protein concentrations with a quantile regression model. Significance was set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>DDwoR patients had smaller preoperative mouth opening capacity (p &lt; 0.001) and shorter symptom duration (p = 0.007) compared to DDwR. Patients with a sudden onset of DDwoR more often had trauma to the jaws as an inciting event compared to delayed onset DDwoR (p = 0.014). In the adjusted analyses, ADAMTS13 (p = 0.000), aggrecan (p = 0.012), collagen IVα1 (p = 0.001), Lumican (p = 0.008), MMP-7 (p = 0.000), MMP-10 (p = 0.043), NCAM-1 (p = 0.006), Tenascin C (p = 0.030), TIMP-2 (p = 0.000), and TIMP-3 (p = 0.027) had significantly higher concentrations in DDwoR. MMP-9 (p = 0.019) and syndecan-4 (p = 0.002) demonstrated a positive strong correlation to female gender. A higher Wilkes criteria corresponded to a higher protein composition similarly to how protein composition increased in DDwoR compared to DDwR.</div></div><div><h3>Conclusion</h3><div>ECM protein composition changes with TMJ diagnoses and Wilkes criteria suggesting degenerative changes as a main factor. Gender seems to marginally affect the ECM.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106338"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a liquid-liquid phase separation-related genes signature to predict clinical outcomes, tumor immune microenvironment and chemotherapeutic response in head and neck squamous cell carcinoma","authors":"Pin Lv ,&nbsp;Yang Zhang ,&nbsp;Yuhan Wang,&nbsp;Pengfei Diao,&nbsp;Yaping Wu,&nbsp;Jie Cheng","doi":"10.1016/j.archoralbio.2025.106334","DOIUrl":"10.1016/j.archoralbio.2025.106334","url":null,"abstract":"<div><h3>Objective</h3><div>Liquid-liquid phase separation (LLPS) has been linked to oncogenesis, prompting research into LLPS-related genes as prognostic markers. Here, our objective was to develop a prognostic signature specific to head and neck squamous cell carcinoma (HNSCC) utilizing genes associated with LLPS.</div></div><div><h3>Design</h3><div>The LRG signature was developed using transcriptional sequence data obtained from the TCGA and GEO databases, employing univariate Cox regression, Kaplan-Meier analysis, and LASSO algorithms. Additionally, we developed an LRG nomogram that integrates the signature with specific clinicopathological features, identified using multivariate Cox regression. Clinical value of LRG signature in predicting chemotherapeutic drug sensitivity and immune infiltration was evaluated by bioinformatics approaches. One of the genes in the signature was knocked down to confirm its role <em>in vitro</em>.</div></div><div><h3>Results</h3><div>The LRG signature demonstrated prognostic performance across multiple independent cohorts. Moreover, LRG signature scores exhibited a negative correlation with the presence of tumor-infiltrating immune cells and were associated with the sensitivities of various chemotherapeutic agents. LRP12 silencing markedly inhibited the proliferation, migration, and invasion of HNSCC cells, while also inducing apoptosis in these cells.</div></div><div><h3>Conclusion</h3><div>The findings of our study underscore the efficacy of the LRG signature and nomogram in forecasting prognosis and response to chemotherapy, suggesting a favorable outlook for their application in clinical settings.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106334"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A cone beam computed tomography study on the osseous morphology of temporomandibular joint with free loss of posterior teeth","authors":"Meiling Chen ,&nbsp;Yaxin Lin ,&nbsp;Ran Wei ,&nbsp;Yanjing Li ,&nbsp;Ruimeng Yang ,&nbsp;Zheng Wang ,&nbsp;Peng Li ,&nbsp;Lei Sui","doi":"10.1016/j.archoralbio.2025.106337","DOIUrl":"10.1016/j.archoralbio.2025.106337","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this retrospective study is to explore the association between free loss of posterior teeth (FLP) including bilateral (BFLP) and unilateral (UFLP) free loss of posterior teeth and the osseous morphology of temporomandibular joint (TMJ).</div></div><div><h3>Design</h3><div>The condylar volume(V) and surface area(S), condylar mediolateral diameter (CD), horizontal condylar angle (HCA), glenoid fossa depth (GFD) and articular eminence inclination (AEI) of the bilateral TMJs were measured by using cone-beam computed tomography (CBCT) and Mimics software. The study included 120 FLP patients (UFLP=60, BFLP=60) and 60 controls with complete dentition. The differences between and within groups were statistically analyzed by SPSS 26.0. A two-way ANCOVA was employed to analyze the interaction effects among FLP, sex, and age on TMJ osseous morphological changes.</div></div><div><h3>Results</h3><div>In the UFLP group, both CD and AEI of the missing side were greater than those of the non-missing side(<em>p</em> &lt; 0.05). Between groups, the GFD of the control group was significantly greater than those of the BFLP group and the missing side and non-missing side of the UFLP group (<em>p</em> &lt; 0.05). Two-way ANCOVA demonstrated significant sex-based differences in TMJ morphology (V, S, CD, GFD, AEI; <em>p</em> &lt; 0.05), with pronounced FLP × sex interactions for V, S, and CD (<em>p</em> &lt; 0.001). Age predominantly influenced GFD (<em>p</em> &lt; 0.001), surpassing FLP and sex effects (<em>p</em> &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>FLP was associated with shallower glenoid fossa morphology. Patients with UFLP showed asymmetric TMJs, with reduced CD and a flatter articular eminence on the non-missing teeth side. FLP-associated TMJ changes require careful consideration of sex and age factors.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106337"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dodecylmethylaminoethyl methacrylate inhibits the growth of Candida albicans and Enterococcus faecalis biofilm and the formation of osteoclast","authors":"Zong Yawen ,&nbsp;Shan Tiantian ,&nbsp;Shi Yangyang ,&nbsp;Li Yiling ,&nbsp;Lai Siyue ,&nbsp;Wei Yu ,&nbsp;Guo Xiao ,&nbsp;Li Bolei ,&nbsp;Ren Biao ,&nbsp;Cheng Lei","doi":"10.1016/j.archoralbio.2025.106331","DOIUrl":"10.1016/j.archoralbio.2025.106331","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the antimicrobial ability on the dual-species biofilm formed by <em>C. albicans</em> and <em>E. faecalis</em> and osteoclysis inhibition of DMAEM.</div></div><div><h3>Design</h3><div>DMAEM monomer was used to evaluate the antmicrobial ability on the dual-species biofilm of <em>C. albicans</em> and <em>E. faecalis</em> by biomass quantification, Scanning electron microscope and confocal laser scanning microscopy scanning, and RT-qPCR. The biosafty and inhibition ability on the dual-species biofilm and osteoclysis of the experimental root canal sealers containing DMAEM was tested by biomass quantification, Cell Counting kit-8, tartrate-resistant acid phosphatase staining, flow cytometry analysis, and RT-qPCR.</div></div><div><h3>Results</h3><div>The biomass of the dual-species biofilm formed by <em>C. albicans</em> and <em>E. faecalis</em> was significantly decreased by 26.3–51.3 % under 8–256 μg/mL DMAEM. 8 μg/mL DMAEM could reduce <em>E. faecalis’</em>s survival rate to 77.4 % (p &lt; 0.001) and down-regulated its virulence gene expression. Meanwhile, the mycelium count proportion of <em>C. albicans</em> in the dual<em>-</em>species biofilm was reduced from 50 % to 0 % (p &lt; 0.0001). The experimental sealers with DMAEM content could also decrease the survival rate of <em>C.albicans</em> and <em>E.faecalis</em> in the dual-species biofilm. Moreover, the elution of experimental root canal sealers containing ≤ 2.5 % DMAEM was biosafe and improved 81.0 % (p &lt; 0.05) osteoclastic inhibition compared with it of unmodified sealers at least.</div></div><div><h3>Conclusions</h3><div>The antimicrobial ability on the dual-species biofilm formed by <em>C. albicans</em> and <em>E. faecalis</em> and osteoclastic inhibition of DMAEM suggested its potential clinical application of DMAEM in the treatment of periapical inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106331"},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis promotes oral carcinogenesis through the NDK-ATP-P2X7 signal axis: An in vitro experimental study","authors":"Wei Wei ,&nbsp;Chen‑xi Li ,&nbsp;Mu-qiu Li ,&nbsp;Xiao-rong Tan ,&nbsp;Zhong‑cheng Gong","doi":"10.1016/j.archoralbio.2025.106332","DOIUrl":"10.1016/j.archoralbio.2025.106332","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate how <em>Porphyromonas gingivalis</em> (P. g) secreted nucleoside diphosphate kinase (NDK) affects the tumor-related biological behaviors of oral squamous cell carcinoma (OSCC) cells through NDK-ATP-P2X7 signal axis.</div></div><div><h3>Design</h3><div>An in vitro co-culture model that includes human OSCC cell lines (SCC 9 and SCC 25) together with P. g (W83 strain) and its NDK knockout variant (P. g-△NDK) were established to perform various key experiments to evaluate multiple malignant phenotypes, as well as analyze the relationship between adenosine triphosphate (ATP) and purinergic ligand-gated ion channel 7 (P2X7) receptor.</div></div><div><h3>Results</h3><div>In OSCC tissues, the levels of P. g and P2X7 were significantly higher than those found in adjacent normal tissues (<em>P</em> &lt; 0.0001). In cytological experiments, SCC9 and SCC25 cells infected with P. g exhibited enhanced proliferation, migration, and invasion capabilities (<em>P</em> &lt; 0.0001), increased cytotoxicity (<em>P</em> &lt; 0.0001), and decreased extracellular ATP content (<em>P</em> &lt; 0.001). After infection with <em>P</em>. g-△NDK, SCC9 and SCC25 cells showed enhanced proliferation, migration, and invasion abilities (<em>P</em> &lt; 0.0001), reduced cytotoxicity (<em>P</em> &lt; 0.0001), and increased extracellular ATP content (<em>P</em> &lt; 0.01). There was a positive correlation between P2X7 expression and extracellular ATP content; in contrast, adding a P2X7 inhibitor produced opposite results (all <em>P</em> &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>In SCC 9 and SCC 25 cells, P. g-△NDK reduces the hydrolysis of extracellular ATP by P. g, which enhances P2X7 expression and results in improved cellular proliferation, migration, and invasion capabilities, along with decreased cytotoxicity.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106332"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between smoking, disease, and Porphyromonas gingivalis fimA genotype distribution: Insights from Korean periodontitis patients","authors":"Eun-Young Jang ,&nbsp;Seok Bin Yang ,&nbsp;Kyu Hwan Kwack ,&nbsp;Jae-Hyung Lee ,&nbsp;Ji-Hoi Moon","doi":"10.1016/j.archoralbio.2025.106333","DOIUrl":"10.1016/j.archoralbio.2025.106333","url":null,"abstract":"<div><h3>Objective:</h3><div>To investigate the distribution of <em>Porphyromonas gingivalis fimA</em> genotypes in relation to smoking and periodontitis severity in a Korean adult population, and to assess genotype-specific associations with host and environmental factors.</div></div><div><h3>Design:</h3><div>Subgingival biofilm samples were obtained from 283 adults, comprising 102 non-smoking healthy individuals, 98 non-smoking periodontitis patients, and 83 smoking periodontitis patients. Bacterial DNA was extracted and analyzed using <em>fimA</em>-specific PCR to detect <em>P. gingivalis</em> and its six <em>fimA</em> genotypes (I, II, III, IV, V, and Ib). Statistical analyses were performed using Fisher’s exact test and logistic regression models.</div></div><div><h3>Results:</h3><div>Type II <em>fimA</em> was the most common genotype across all groups and was significantly more prevalent in periodontitis patients. Fisher’s exact test revealed significant differences in genotype distribution between healthy and diseased individuals, except for types I and III. Logistic regression showed that <em>P. gingivalis</em> detection was strongly associated with disease stage (odds ratio: 7.5), with type II <em>fimA</em> linked to advanced disease. Types IV and Ib were significantly associated with smoking and age, suggesting differential responses of specific genotypes to environmental exposure.</div></div><div><h3>Conclusions:</h3><div>Given the distinct associations between <em>fimA</em> genotypes and smoking, careful consideration of strain selection is crucial for future studies investigating <em>P. gingivalis</em> pathogenesis. This study underscores the importance of genotype-specific microbial analyses in elucidating mechanisms of periodontitis progression and highlights the potential for environmental factors such as smoking to shape microbial variation at the strain level.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106333"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular advances in adenoid cystic carcinoma: Genetic and epigenetic insights","authors":"Luciana Oliveira Almeida ,&nbsp;Kevin Luiz Lopes-Delphino ,&nbsp;Isabel Schausltz Pereira Faustino ,&nbsp;Lara Maria Alencar Ramos Innocentini ,&nbsp;Luiz Carlos Conti de Freitas ,&nbsp;Pedro Polastri Lima Peixoto ,&nbsp;Ana Carolina Fragoso Motta ,&nbsp;Lucas Dias de Oliveira ,&nbsp;Rogério Moraes Castilho ,&nbsp;Katiuchia Uzzun Sales","doi":"10.1016/j.archoralbio.2025.106323","DOIUrl":"10.1016/j.archoralbio.2025.106323","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to provide an overview of genetic and epigenetic alterations in adenoid cystic carcinoma, addressing challenges in prognosis, treatment, recurrence, and multidisciplinary management.</div></div><div><h3>Design</h3><div>A narrative review was conducted through searches in the PubMed, Scopus, Web of Science, EMBASE, LILACS and Google Scholar databases, using relevant keywords and including the verification of reference lists from journal articles through manual searching.</div></div><div><h3>Results</h3><div>The MYB-NFIB fusion emerged as a hallmark genetic feature of adenoid cystic carcinoma, influencing tumor behavior and resistance to apoptosis. Alterations in the PI3K/AKT and NOTCH pathways contributed to adenoid cystic carcinoma’s aggressive traits, such as perineural invasion and metastasis. Epigenetic modifications, including DNA methylation and histone changes, were also linked to tumor progression and poor prognosis. Proteases such as MMP-9 and cathepsins D and B are associated with aggressive disease and prognosis. Although adenoid cystic carcinoma's rarity challenges clinical trials, these molecular markers remain valuable therapeutic targets.</div></div><div><h3>Conclusion</h3><div>The molecular complexity of adenoid cystic carcinoma underscores the need for targeted therapies that address its specific genetic and epigenetic landscape. Future research should prioritize clinical trials focusing on molecularly targeted treatments and epigenetic interventions' role in improving adenoid cystic carcinoma outcomes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106323"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144255040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Permanent dental development radiographically assessed in children and adolescents with down syndrome - systematic review and meta-analysis","authors":"Maria Isabel Gomes França ,&nbsp;Debora Duarte Moreira ,&nbsp;Carolina Lima Lopes ,&nbsp;Fabiana Mantovani Gomes França ,&nbsp;Rizky Merdietio Boedi ,&nbsp;Ademir Franco","doi":"10.1016/j.archoralbio.2025.106322","DOIUrl":"10.1016/j.archoralbio.2025.106322","url":null,"abstract":"<div><h3>Objective</h3><div>To systematically revisit the existing scientific literature and investigate whether Down syndrome can affect the chronology of dental development in children and adolescents.</div></div><div><h3>Design</h3><div>A systematic literature review based on the Joanna Briggs Institute (JBI) Manual for Evidence Synthesis was developed and registered in The International Prospective Register of Systematic Reviews (PROSPERO, protocol: CRD #42024531685). The research protocol was designed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) and deposited in Zenodo repository (doi: 10.5281/zenodo.14993171). The strategic search was conducted in PubMed, LILACS, Scopus, SciELO, OATD and Open Grey. Risk of bias was assessed according to the JBI critical appraisal tool, while The Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool was used to assess the certainty of evidence. Subgroup meta-analyses were performed based on sex and dental age estimation method.</div></div><div><h3>Results</h3><div>Ten eligible studies were detected after 1864 initially screened. Standardized mean differences (SMD) between syndromic and non-syndromic individuals were not statistically significant (p &gt; 0.05). Differences between chronological and estimated ages were negligible (p &gt; 0.05) for both males and females. Nolla’s dental age estimation method was slightly more conservative and consistent than Demirjian’s (p &gt; 0.05). Heterogeneity (I²) was ≤ 3 %.</div></div><div><h3>Conclusion</h3><div>Down syndrome does not seem to significantly affect dental development of children and adolescents with and without Down syndrome when assessed radiographically. Caution is advised since the certainty of evidence of observational studies is naturally low.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106322"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary proteome and microbiome in pregnancy and postpartum: An exploratory study on the relation with arterial hypertension","authors":"Maria João Azevedo ,&nbsp;Ana Filipa Ferreira ,&nbsp;Dmitry Loginov ,&nbsp;Petr Novák ,&nbsp;Inês Falcão-Pires ,&nbsp;Carla Ramalho ,&nbsp;Mark J. Buijs ,&nbsp;Bernd W. Brandt ,&nbsp;Egija Zaura ,&nbsp;Fábio Trindade ,&nbsp;Benedita Sampaio-Maia","doi":"10.1016/j.archoralbio.2025.106319","DOIUrl":"10.1016/j.archoralbio.2025.106319","url":null,"abstract":"<div><h3>Objectives</h3><div>Arterial hypertension (AH) influences salivary gland physiology and oral health, being associated with a higher incidence of periodontal disease in pregnant women. Evidence points to a bidirectional relationship between the oral microbiota and blood pressure regulation. Therefore, this study aimed to characterize the oral health of pregnant women and AH-associated changes in the salivary proteome and microbiome during pregnancy and postpartum.</div></div><div><h3>Design</h3><div>Ten healthy women and ten women with AH were enrolled. Saliva was collected during pregnancy and six months postpartum. The salivary proteome was characterized by shotgun label-free mass spectrometry analysis. Specific proteins were validated through parallel reaction monitoring (PRM). The oral microbiota was characterized via 16S rRNA gene amplicon sequencing (V4 region). The periodontal health and the caries history was assessed during pregnancy.</div></div><div><h3>Results</h3><div>Pregnant women with AH had lower junction plakoglobin (JUP)- and desmoplakin (DSP)-specific peptide levels than healthy women, confirmed by the PRM approach. The levels of these proteins correlated negatively with periodontal health indexes, which were higher in pregnant women with AH. In AH, nitrate-reducing microorganisms had lower abundance, correlating positively with JUP and DSP-specific peptides.</div></div><div><h3>Conclusions</h3><div>The salivary proteome and microbiota are shaped by AH during and after pregnancy. Further research is required to understand the underlying mechanisms impairing oral health in AH.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106319"},"PeriodicalIF":2.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}