Archives of oral biology最新文献

{"title":"Branched-chain amino acids promote gelatinase secretion from human periodontal ligament stem cells through nuclear factor kappa-B signaling","authors":"Xinjie Ning ,&nbsp;Huiling Zheng ,&nbsp;Ying Tu ,&nbsp;Qiang Guo ,&nbsp;Biao Ren ,&nbsp;Leng Wu ,&nbsp;Jing Xie ,&nbsp;Chengcheng Liu","doi":"10.1016/j.archoralbio.2025.106297","DOIUrl":"10.1016/j.archoralbio.2025.106297","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the effects of branched-chain amino acids (BCAAs) on periodontal tissues and regulation of gelatinase secretion by human periodontal ligament stem cells (hPDLSCs).</div></div><div><h3>Design</h3><div>The salivary BCAA levels (leucine, isoleucine, and valine) in the clinical participants were measured using mass spectrometry. A local injection model in the periodontium of Sprague Dawley rats was established to investigate the periodontal destruction induced by BCAAs. A BCAA-treatment model of hPDLSCs was established to detect the expression and activity of gelatinase and further explore the potential mechanism by which BCAAs enhance gelatinase secretion.</div></div><div><h3>Results</h3><div>Compared to the healthy controls, the salivary levels of leucine (<em>p</em> = 0.0190), isoleucine (<em>p</em> = 0.0351), and valine (<em>p</em> = 0.0072) were significantly elevated in individuals with periodontitis. <em>In vivo</em> experiments revealed that BCAAs aggravated periodontal extracellular matrix degradation and alveolar bone resorption in rats. Three-dimensional reconstruction of the rat maxilla demonstrated an increase in the distance from the cementoenamel junction to the alveolar bone crest (<em>p</em> &lt; 0.0001), and a decrease in the bone volume fraction (<em>p</em> &lt; 0.0001). <em>In vitro</em> experiments demonstrated that BCAAs activate the phosphorylation of nuclear factor kappa-B (NF-κB) signaling pathway in the hPDLSCs and consequently induce the secretion of gelatinases. The absence of any of the components in the BCAAs attenuated this effect.</div></div><div><h3>Conclusion</h3><div>BCAAs increase gelatinase secretion through the NF-κB (p-p65) signaling pathway, consequently exacerbating periodontal tissue destruction. This provides a novel insight on the role of BCAAs in the host immune-inflammatory response and increases our understanding of the possible involvement of BCAAs in the periodontitis development.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106297"},"PeriodicalIF":2.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Porphyromonas gingivalis lipopolysaccharide on the behavior of human dental pulp stem cells in vitro and their inflammation-related transcriptomics profile","authors":"Fei He ,&nbsp;Jingya Zhu ,&nbsp;Xiangni Zeng ,&nbsp;Li Jiang ,&nbsp;Lixia Zhang","doi":"10.1016/j.archoralbio.2025.106295","DOIUrl":"10.1016/j.archoralbio.2025.106295","url":null,"abstract":"<div><h3>Objective</h3><div>The inflammatory microenvironment in pulpitis and periapical periodontitis critically impairs dental pulp stem cells (DPSCs) functionality, yet the underlying molecular mechanisms remain poorly defined. This study aimed to investigate the effects of <em>Porphyromonas gingivalis</em> lipopolysaccharide (<em>P. gingivalis</em> LPS) on human DPSCs behavior and elucidate the transcriptomic changes underlying LPS-induced inflammation.</div></div><div><h3>Design</h3><div>Human DPSCs were exposed to 1 µg/mL LPS for 24 hours to establish an inflammatory model, with subsequent evaluation of proliferation (CCK-8), migration (Transwell), and odontogenic differentiation (ALP staining, mineralization assays and odontoblast markers expression). Cell cycle progression was quantified through flow cytometry, while RNA sequencing analysis delineated transcriptional alterations.</div></div><div><h3>Results</h3><div>LPS exposure significantly attenuated DPSCs proliferative capacity (<em>P</em> &lt; 0.01), suppressed migration (<em>P</em> &lt; 0.01), and impaired odontogenic differentiation, evidenced by diminished ALP activity, mineralized nodule formation and odontoblast markers expression. Cell cycle analysis revealed G0/G1 phase arrest (<em>P</em> &lt; 0.01), indicating proliferative quiescence. Transcriptomic profiling identified 467 differentially expressed genes. <em>P. gingivalis</em> LPS exerts its effects on DPSCs by concurrent activation of both canonical (Toll-like receptors, TLRs) and non-canonical (Nucleotide-binding oligomerization domain-like receptor) signaling pathway. Pathway enrichment revealed the enrichment of the several important signaling pathways such as Phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt), tumor necrosis factor (TNF) /Nuclear factor kappa B (NF-κB) and interleukin (IL) −17 signaling under LPS stimulation.</div></div><div><h3>Conclusion</h3><div><em>P. gingivalis</em> LPS disrupts DPSCs regenerative functions by modulating inflammatory and developmental signaling pathways, providing mechanistic insights into impaired pulp repair during oral infections.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106295"},"PeriodicalIF":2.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of experimental polymer-containing solutions in preventing caries-like lesions on enamel","authors":"Raissa Manoel Garcia ,&nbsp;Astrid Carolina Valdivia Tapia ,&nbsp;Alessandra Buehler Borges ,&nbsp;George J. Eckert ,&nbsp;Frank Lippert ,&nbsp;Tais Scaramucci Forlin ,&nbsp;Anderson T. Hara","doi":"10.1016/j.archoralbio.2025.106286","DOIUrl":"10.1016/j.archoralbio.2025.106286","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate experimental solutions containing polymers (Chitosan: CHI, Sodium linear polyphosphate: LPP, Polyacrylic acid: PAA; and 2-methacryloyloxyethyl phosphorylcholine: MPC) isolated, associated or not with fluoride (F), stannous ions (Sn), or F+Sn <em>in vitro</em> against caries-like lesion in enamel.</div></div><div><h3>Design</h3><div>Bovine enamel sections were randomly allocated into 20 groups (n = 14): Distilled water (DIW, negative control), F: 220 ppm F^-, Sn: 800 ppm Sn²⁺, F+Sn, and four polymers (0.5 % CHI, 2 % LPP, 0.1 % PAA, 2 % MPC) associated or not to F, Sn, or F+Sn. The single sections were individually subjected to a pH cycling: demineralization (3 h, 2 ×/d), experimental solution (1 min, 2 ×/d), and remineralization (overnight), for 5d. The enamel sections were analyzed using digital transverse microradiography (integrated mineral loss-ΔZ, and lesion depth-L). Data were analyzed using Kruskal-Wallis and Wilcoxon rank sum tests, with alpha= 0.05.</div></div><div><h3>Results</h3><div>When tested in isolation, LPP was the only polymer affording greater protection than DIW, while PAA enhanced ΔZ [median (Q1-Q3); vol%min× µm: 2530 (2070–3140) vs. 3710 (3410–3860) vs. 4375 (3820–4990) respectively; all p &lt; 0.05]. None of the polymers could increase the protection provided by F [1430 (1340,1570), p = 0.084] or F+Sn [1515(1150–1940), p = 0.182)]. CHI [2525(1890–3210), p = 0.01] and LPP [3105 (2460–3250), p = 0.006] increase the protection offered by Sn [3500 (3350–3790)], but PAA enhanced ΔZ [4340 (3540–4820), p = 0.044]. For L data, there are no polymer effects compared to DIW [93.30(82.60–104.80), p = 0.177], F [78.25(70.40–90.30), p = 0.340] or F+Sn [73.05(54.60–81.00), p = 0.056]. Only MPC decrease the degree effect of Sn [113.10 (110.40–124.30) and 99.20(92.60, 105.80), p = 0.003].</div></div><div><h3>Conclusions</h3><div>LPP was the only polymer that protected enamel against demineralization when tested in isolation. However, none of the polymers could increase the protection offered by fluoride or fluoride plus stannous ions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106286"},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distributions of orofacial two-point discrimination threshold: A cross-sectional study in healthy population and patients with pain-related temporomandibular disorders","authors":"Yifei Long,&nbsp;Yihang Fu,&nbsp;Tianyi Chen,&nbsp;Yunhao Zheng,&nbsp;Li Chen,&nbsp;Tiqian Liu,&nbsp;Jun Wang,&nbsp;Xin Xiong","doi":"10.1016/j.archoralbio.2025.106284","DOIUrl":"10.1016/j.archoralbio.2025.106284","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the distribution and difference of orofacial two-point discrimination threshold (2-PDT) between patients with pain-related Temporomandibular Disorders (TMDs) and healthy individuals.</div></div><div><h3>Methods</h3><div>This study included 58 pain-related TMDs patients and 58 healthy individuals. Demographics including the gender, age of participants were collected. Temporomandibular joint (TMJ), mandible, zygomatic and temporal 2-PDTs were examined for statistical analyses.</div></div><div><h3>Results</h3><div>Pain-related TMDs patients showed significantly higher 2-PDTs in all tested regions (<em>p</em> &lt; 0.001). In gender subgroups of the control, males had significantly higher 2-PDTs than females in bilateral TMJ and temporal regions. In gender subgroups of patients, no statistical 2-PDT differences were found between males and females. Pain-related TMDs females had higher 2-PDTs than control females in all regions, which were also observed between pain-related TMDs males and control males (<em>p</em> &lt; 0.001), except for temporal 2-PDTs. Only pain-related TMDs were significantly associated with orofacial 2-PDTs with β coefficients varied from 3.5 to 7 (<em>p</em> &lt; 0.001). The discrimination performance of 2-PDTs on pain-related TMDs was considered effective, while the TMJ 2-PDTs was the best single predictor (Area under curve: 0.833).</div></div><div><h3>Conclusion</h3><div>Pain-related TMDs patients had weaker orofacial tactile acuity, which was consistent in gender subgroups, except for the temporal region of males. Healthy males had weaker tactile acuity compared to females in TMJ and temporal regions. No significant gender effect on orofacial tactile acuity was observed among male and female patients. The 2-PDTs of the tested sites, especially for TMJ, has the potential for further utilization in pain-related TMDs patients.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106284"},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of dose and time effects of flavonoids, alone or in combination, on the induction of mineralization markers in human osteoblast-like cells","authors":"Rafaela Laruzo Rabelo ,&nbsp;Gabriel Pereira Nunes ,&nbsp;Geórgia Rondó Peres ,&nbsp;Rafael Araújo Rios ,&nbsp;Maria Eduarda de Souza ,&nbsp;Gabriela Pacheco de Almeida Braga ,&nbsp;Cristiane Duque","doi":"10.1016/j.archoralbio.2025.106285","DOIUrl":"10.1016/j.archoralbio.2025.106285","url":null,"abstract":"<div><h3>Objective</h3><div>This study compared the cytotoxicity, alkaline phosphatase (ALP) activity, and mineralization-inducing effects of flavonoids on human osteoblast-like Saos-2 cells.</div></div><div><h3>Design</h3><div>Saos-2 cells were treated with quercetin, myricetin, pinocembrin, kaempferol, isoquercitrin (Iso), rutin (Rut), taxifolin (Tax), ampelopsin (Amp), epigallocatechin-3-gallate (EGCG), and chrysin at 100, 50, and 25 µM for 48 h. Metabolic activity, ALP activity and mineral deposition were assessed via resazurin, thymolphthalein and Alizarin Red S staining assays, respectively, after 8 and 14 days. Calcium hydroxide was used as positive control, and untreated cells (DMEM) as negative control. Based on initial screening, Tax, Iso, Rut and Amp were selected for double combination treatments (at 50/50 µM and at 25/25 µM), and the same assays were performed. Data were analyzed using ANOVA and Tukey's test (α = 0.05).</div></div><div><h3>Results</h3><div>Comparing the flavonoids, regardless of time and concentration, only chrysin at 100 µM and 50 µM significantly reduced cell viability. Iso, Rut, Tax, and Amp exhibited the highest ALP activity and mineralized nodules formation, significantly outperforming the other flavonoids and DMEM control, particularly at 50 and 25 µM (p &lt; 0.05). Among the combinations, Tax+Iso, Tax+Amp and Tax+Rut, at 25/25 µM demonstrated higher ALP activity and enhanced mineral deposition compared to the other flavonoid combinations and DMEM (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Based on this preliminary in vitro model, Tax, Iso, Rut, and Amp, both individually and in combination, effectively promote biomineralization in Saos-2 cells, without inducing cytotoxic effects. These flavonoids hold significant potential for osteogenic applications, warranting further in vivo studies and clinical trials to optimize their therapeutic use.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106285"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of omega-3 and omega-6 fatty acids on tumor suppressor pathways in mice tongue oral epithelial dysplasia","authors":"Julieta Don ,&nbsp;Victoria Ferrero ,&nbsp;Tamara Mazo ,&nbsp;Sol Bernárdez ,&nbsp;Nelso Barotto ,&nbsp;Mabel Brunotto ,&nbsp;María Eugenia Pasqualini","doi":"10.1016/j.archoralbio.2025.106283","DOIUrl":"10.1016/j.archoralbio.2025.106283","url":null,"abstract":"<div><h3>Objective</h3><div>This work aimed to evaluate the modulating effect of polyunsaturated fatty acids omega-3 and omega-6 on the expression of TP53 and specific primiRNAs 34 b/c in the development of premalignant characteristics in tongue epithelial dysplasias in-vivo experimental models</div></div><div><h3>Design</h3><div>BALB/c mice were divided into two diet groups: fish oil rich in omega-3 and corn oil rich in omega-6. Tongue dysplasia was generated by applying a 0.5 % solution of 7,12-dimethylbenzantracene (DMBA) in acetone. After sacrificing, the animals were assessed for omega-6 and omega-3, TP53/Ki67 immunostaining, Trp53R270H mutations, and primiRNA 34 b/c expression.</div></div><div><h3>Results</h3><div>Mice fed omega-6 showed higher dysplasias and Ki67 immunostaining percentages than those fed omega-3-rich oils. Furthermore, mice fed with corn oil rich in omega-6 showed significantly high average arachidonic-acid values, while the group fed cod oil rich in omega-3 showed substantially higher average values of Ecosapentanoic acid and Docosahexanoic acid in tongue tissue cell membranes. The primiRNA-34 b/c was upregulated in animals fed corn oil, enriched-omega-6 at 90 days.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that dietary lipids influence the development of DMBA-induced premalignant features on the tongue of mice; therefore, a low intake of omega-6-rich oils and a higher intake of omega-3 could be recommended for preventing features similar to mucosal dysplasia, which could be an important preventive strategy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106283"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in oral microbiota between elderly and adults: A systematic review","authors":"Natalia S. Rozas ,&nbsp;Nicole Stephens ,&nbsp;Korina Yun-Fan Lu ,&nbsp;Cameron B. Jeter","doi":"10.1016/j.archoralbio.2025.106278","DOIUrl":"10.1016/j.archoralbio.2025.106278","url":null,"abstract":"<div><h3>Objective</h3><div>The aging process may significantly impact bacteria in the oral cavity (oral microbiome), leading to notable changes in its diversity and composition, potentially influencing systemic health. This systematic review aimed to compare the oral microbiota of elderly individuals (≥60 years) with that of younger adults.</div></div><div><h3>Design</h3><div>A comprehensive search of PubMed, Medline, EMBASE and CINAHL identified 794 studies published between 2005 and May 2023. Studies were included if the investigators compared the oral microbiome of elderly with younger adults using 16S rRNA sequencing. The JBI Critical Appraisal Checklist for Analytical Cross-Sectional Studies was employed to evaluate risk of bias. Ten full-text articles met the criteria for inclusion.</div></div><div><h3>Results</h3><div>The included studies revealed inconsistent results regarding alpha and beta diversity and the abundance of bacterial phyla with aging. Some studies reported increased diversity or abundance, others showed decreases, and several found no significant differences. However, this review identified a consistent decline in the abundance of <em>Haemophilus</em>, <em>Granulicatella</em>, and <em>Veillonella</em>, alongside an increase in <em>Comamonas</em>, in elderly populations across multiple studies. These genera may play critical roles in maintaining oral health and microbial stability in elderly populations, highlighting the need for further investigation.</div></div><div><h3>Conclusions</h3><div>The variability in microbial diversity and abudance suggests that confounding factors such as oral hygiene, systemic health, medication use, and lifestyle may complicate interpretation of results. In conclusion, future research should employ longitudinal designs with standardized methodologies and address confounding factors to better understand the aging oral microbiome and its implications for healthspan and quality of life.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106278"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming growth factor-β1 and its soluble receptor type 2 in saliva of young adults: Sex-related differences and predictive modeling of salivary concentrations","authors":"Marija S. Milić ,&nbsp;Bojan Dželetović ,&nbsp;Biljana Anđelski Radičević ,&nbsp;Nataša Milosavljević ,&nbsp;Nina Dimitrijević Jovanović ,&nbsp;Ivan Dožić ,&nbsp;Jelena Krunić ,&nbsp;Ljiljana Đukić","doi":"10.1016/j.archoralbio.2025.106279","DOIUrl":"10.1016/j.archoralbio.2025.106279","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate and compare salivary presence and levels of Transforming Growth Factor-β1 (TGF-β1) and its soluble receptor type 2 (TGFBR2), along with the biochemical profile of the unstimulated whole saliva (UWS) of healthy young males and females; and to assess the potential of the predictive modeling for estimating the active TGF-β1 and TGFBR2 levels based on sex and individual salivary biochemical profile.</div></div><div><h3>Design</h3><div>Study sample included 20 participants, both sexes, with the median value of age being 20.00 (1.00) years. Total and active TGF-β1 and TGFBR2 levels were tested with ELISA. Biochemical analysis of saliva, including lactat dehydrogenase (LDH) activity and uric acid levels, was conducted via spectrophotometry. Salivary pH and buffer capacity were determined with potentiometry. To model the relationship of active TGF-β1 and TGFBR2 levels with sex and individual salivary biochemical profiles, multivariate regression analysis was employed.</div></div><div><h3>Results</h3><div>The median/mean values of active TGF-β1 (33.13 (27.26) vs. 14.24 (9.89) pg/ml, p = 0.013), uric acid (260.00 (136.00) vs. 199.00 (74.50) μmol/L, p = 0.031), and LDH activity (82.00 ± 45.23 vs. 42.40 ± 30.99 U/L, p = 0.035) were significantly higher in males vs. females, respectively. Multivariate regression method demonstrated 71.9 % accuracy in predicting the levels of active TGF-β1, while for TGFBR2 the accuracy was 86.01 %.</div></div><div><h3>Conclusions</h3><div>Salivary levels of active TGF-β1 are significantly higher in young healthy males compared to females. The multivariate regression model demonstrates promising predictive potential for estimating the levels of active TGF-β1 and TGFBR2 in young healthy individuals, based on sex and individual salivary biochemical profiles.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106279"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical expression of p53/TP53 in histologically negative mucosal margins of head and neck squamous cell carcinomas and usefulness in predicting locoregional recurrence: A systematic review and meta-analysis","authors":"Gopikrishnan Vijayakumar ,&nbsp;Sithara Aravind ,&nbsp;Anand Vijaya Narayanan ,&nbsp;Mala Kamboj ,&nbsp;Sangeeta K. Nayanar ,&nbsp;Adarsh Kumar ,&nbsp;Anjali Narwal","doi":"10.1016/j.archoralbio.2025.106281","DOIUrl":"10.1016/j.archoralbio.2025.106281","url":null,"abstract":"<div><h3>Objective</h3><div>To compare the expression of p53/TP53 in histologically negative mucosal margins of head and neck squamous cell carcinoma with locoregional recurrence.</div></div><div><h3>Design</h3><div>A complete search through MEDLINE by PubMed, Google Scholar, Scopus and EMBASE until 31st January 2023, without period restriction. Articles on head and neck squamous cell carcinoma and p53/TP53 expression were included. Sensitivity, specificity, positive and negative predictive values, positive and negative likelihood ratio, Odds and relative risk ratio of p53/TP53 in histologically negative surgical margins and its effectiveness in predicting recurrences were derived and subjected to meta-analysis using Open Meta analyst for Sierra (10.12) software.</div></div><div><h3>Results</h3><div>The specificity of p53 immunohistochemistry in predicting locoregional recurrence was 0.844 (95 % CI:0.78 ± 0.89) (p-value- &lt; 0.001) with a negative likelihood ratio of 0.487 (95 % CI: 0.35 ± 0.67) (p-value &lt; 0.001) and positive likelihood ratio of 3.032 (95 % CI: 2.17 ± 4.22) (p-value &lt; 0.001). The relative risk of locoregional recurrence in the histologically negative surgical margin with p53 positivity was 3.13 with statistical significance (Odds ratio: 5.249, CI:3.176 ± 8.676, p-value &lt; 0.001). Subgroup meta-analysis based on the location of head and neck SCC shows significant prediction for recurrence in p53 positive margins with more significant results in laryngeal carcinoma recurrence (p value &lt; 0.001) (overall p value &lt; 0.000).</div></div><div><h3>Conclusions</h3><div>Immunohistochemical expression of p53 in histologically negative surgical margins of head and neck squamous cell carcinoma is a significant predictor of locoregional recurrence and prognosis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106281"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From scent to saliva: The saliva-stimulating effect of terpenes in dry mouth","authors":"Wiktoria Potocka ,&nbsp;Zainab Assy ,&nbsp;Annina P. van Splunter ,&nbsp;Marja L. Laine ,&nbsp;Floris J. Bikker","doi":"10.1016/j.archoralbio.2025.106282","DOIUrl":"10.1016/j.archoralbio.2025.106282","url":null,"abstract":"<div><h3>Objectives</h3><div>There is an increasing trend towards the use of bioactive compounds in oral healthcare. Some have been identified as sialagogues, such as mastic resin and its main isolate, α-pinene. These act as acetylcholinesterase inhibitors (AChEIs), consequently increasing salivary flow. With up to half of the global population experiencing dry mouth symptoms, there is a clear need for new interventions.</div></div><div><h3>Design</h3><div>An <em>in vitro</em> screening of a 148-compound library was conducted to identify novel AChEIs. Following this, four compounds were chosen for an <em>in vivo</em> validation and were administered through nasal spray pumps to act as an olfactory stimulus in healthy individuals (n = 12) to evaluate their sialagogic effects. Saliva quantity, rheology, and composition (total protein concentration and MUC5B concentration) were analysed before and after the use of the compounds.</div></div><div><h3>Results</h3><div>This study confirmed α-pinene, and identified basil, eugenol and guaiacol as AChEIs <em>in vitro</em>. Basil and guaiacol significantly increased salivary flow, while simultaneously basil preserved the spinnbarkeit levels. α-Pinene, basil and guaiacol increased the subjective feeling of moistness of the mouth. Total salivary protein concentration was not affected by scent of these compounds, however, α-pinene, basil and guaiacol significantly increased MUC5B output. Furthermore, basil was found to have the most pleasant scent out of the four compounds.</div></div><div><h3>Conclusions</h3><div>In conclusion, this study showed the potency of basil and guaiacol as novel sialagogues. However, further research is necessary to assess the long-term effects and efficacy in patients diagnosed with dry mouth.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106282"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}