Archives of oral biology最新文献

{"title":"Erratum to “Role of Fusobacterium nucleatum in the pathogenesis of oral squamous cell carcinoma: A systematic review of molecular mechanisms and signalling pathways” [Archives of Oral Biology 179 (2025) 106369]","authors":"Bara’a El-Dwaikat , Ikhlas El Karim , Daniel B. Longley , Paul N. Moynagh , Fionnuala T. Lundy","doi":"10.1016/j.archoralbio.2025.106408","DOIUrl":"10.1016/j.archoralbio.2025.106408","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106408"},"PeriodicalIF":2.1,"publicationDate":"2025-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ganoderic acid A attenuates Porphyromonas gingivalis-induced adhesion molecule expression in gingival fibroblasts and tissues via inhibition of NLRP6 inflammasome activation","authors":"Yuanbo Wang ,&nbsp;Jianru Liu ,&nbsp;Xiangying Ouyang ,&nbsp;Wenyi Liu ,&nbsp;Peiying Lyu ,&nbsp;Shengnan Zhang ,&nbsp;Jing Guo","doi":"10.1016/j.archoralbio.2025.106429","DOIUrl":"10.1016/j.archoralbio.2025.106429","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to elucidate the regulatory effects and mechanisms of Ganoderic acid A (GAA) on <em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>)-induced expression of adhesion molecules in human gingival fibroblasts (hGFs) and periodontal tissues, focusing on NLRP6 inflammasome modulation.</div></div><div><h3>Design</h3><div>HGFs were stimulated with <em>P. gingivalis</em> strain W83. The expression levels of NLRP6, caspase-1, IL-1β,IL-18 and adhesion molecules ICAM-1 &amp; VCAM-1 were analyzed by RT-PCR, Western blotting and ELISA, with or without GAA pretreatment. To further explore the regulatory role of GAA on the NLRP6 inflammasome, NLRP6 overexpression assays were performed in hGFs, followed by assessment of ICAM-1 and VCAM-1 expressions. Additionally, a ligature-induced periodontitis mice model was established to evaluate the effects of GAA on NLRP6 expression and inflammatory cell infiltration in periodontal tissues.</div></div><div><h3>Results</h3><div>GAA exhibited no cytotoxicity toward hGFs at low concentrations (within 16 μM). GAA pretreatment significantly inhibited <em>P. gingivalis</em>-induced activation of the NLRP6 inflammasome. Beyond IL-1β and IL-18, it also reduced ICAM-1 and VCAM-1 expression at both mRNA and protein levels. Overexpression of NLRP6 increased the expression of NLRP6 inflammasome components and adhesion molecules, whereas GAA treatment effectively suppressed these elevations. Furthermore, GAA administration markedly downregulated NLRP6 expression and attenuated inflammatory cell infiltration and periodontal inflammation in the mice periodontitis model.</div></div><div><h3>Conclusions</h3><div>GAA attenuates <em>P. gingivalis</em>-induced expression of adhesion molecules by inhibiting NLRP6 inflammasome activation in gingival fibroblasts and periodontal tissues, thereby alleviating inflammatory cell infiltration. These findings suggest the potential of GAA as a therapeutic agent for the management of periodontal inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106429"},"PeriodicalIF":2.1,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical effect of polysaccharide cashew gum on the mitigation of dentin erosion progression under in vitro supraesophageal reflux simulation","authors":"Thaysa Fernandes Pinto Mendes ,&nbsp;Adrianne Caroline Pereira dos Santos Lima ,&nbsp;Eva Aline Costa Cutrim ,&nbsp;Paulo Sérgio de Araújo Sousa ,&nbsp;Durcilene Alves da Silva ,&nbsp;Lucas Antonio Duarte Nicolau ,&nbsp;Leily Macedo Firoozmand","doi":"10.1016/j.archoralbio.2025.106427","DOIUrl":"10.1016/j.archoralbio.2025.106427","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate, <em>in vitro</em>, the topical effect of polysaccharide cashew gum (PLS-cg) on dentine subjected to erosive cycles that simulate refluxate contact in the oral cavity.</div></div><div><h3>Design</h3><div>Human dentine blocks were prepared, standardized, and randomly assigned to one of three treatment groups (<em>n</em> = 10): control group (C) without treatment, commercial dental varnish (CDV) 5 % NaF fluoride varnish, and PLS-cg extract (experimental group). The baseline enamel superficial microhardness (HK) and roughness (Ra) were assessed. The dentin was subjected to erosive cycles (6 × 5 min/day for 9 days) using a hydrochloric acid–pepsin solution (pH 2; 0.75-mg/mL pepsin). Surface roughness (Ra), microhardness (HK), percentage surface hardness loss (%SHL), and topographic analysis with scanning electron microscopy (SEM) were used to evaluate the protective heteropolysaccharide effect. Predictive molecular docking simulations were used to investigate possible interactions between PLS-cg and metalloproteinases (MMP2 and MMP9). The data were analyzed with two-way analysis of variance, <em>t</em> test, Pearson’s correlation, and Tukey’s test (<em>p</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>PLS-cg preserved HK significantly better than the control group (<em>p</em> &lt; 0.001), with the lowest %SHL (-14.04 ± 8.22) and minimal Ra changes (0.124 ± 0.005). Pearson’s correlation between Ra and %SHL was significant and positive (0.7740, <em>p</em> &lt; 0.001). SEM analysis revealed more prominent dentinal tubule openings in the control group, whereas CDV and PLS-cg showed tubule obliteration. Molecular docking identified possible interactions between PLS-cg and MMPs.</div></div><div><h3>Conclusion</h3><div>The findings indicate that PLS-cg may reduce dentine erosion caused by supraesophageal manifestations of gastroesophageal reflux disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106427"},"PeriodicalIF":2.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined bioinformatics and experimental validation identifies m1A-modified genes as potential diagnostic biomarkers in periodontitis and diabetes","authors":"Kai Wang ,&nbsp;Aiguo Wang ,&nbsp;Liewang Qiu ,&nbsp;Ling Xu ,&nbsp;Jingjing Wei","doi":"10.1016/j.archoralbio.2025.106428","DOIUrl":"10.1016/j.archoralbio.2025.106428","url":null,"abstract":"<div><h3>Objective</h3><div>To identify m1A-modified genes with diagnostic potential linking periodontitis and type 2 diabetes mellitus (T2DM) by integrating bioinformatics and experimental validation.</div></div><div><h3>Design</h3><div>Transcriptomic data for periodontitis and T2DM patients were integrated from the GEO database to analyze m1A-related gene expression. A diagnostic model was constructed using ridge and logistic regression. Gene function enrichment, immune infiltration, and protein-protein interaction analyses explored m1A regulatory mechanisms based on m1A scoring and patient clustering models. Gingival tissue samples were collected from periodontitis patients and healthy controls, and a streptozotocin-induced diabetic β-cell model was established. qRT-PCR was performed to validate candidate genes (<em>RRP8</em>, <em>ALKBH3</em>, <em>MAK16</em>, and <em>DDX18</em>). Statistical comparisons were conducted using the non-parametric Mann–Whitney U test.</div></div><div><h3>Results</h3><div>Several m1A-related genes were differentially expressed in both periodontitis and T2DM. <em>RRP8</em> and <em>ALKBH3</em> had high predictive value, with area under the curve (AUC) values of 0.80 (periodontitis) and 0.72 (T2DM). m1A scoring and patient clustering models effectively distinguished patient groups with distinct transcriptomic and immune profiles. Hub genes <em>MAK16</em> and <em>DDX18</em> showed consistent expression patterns and strong correlations with immune infiltration. qRT-PCR confirmed significant downregulation of <em>RRP8</em>, <em>ALKBH3</em>, <em>MAK16</em>, and <em>DDX18</em> in inflamed gingival tissues, and upregulation in the diabetic cell model (<em>p</em> &lt; 0.05), supporting the bioinformatics findings.</div></div><div><h3>Conclusions</h3><div>This integrative study identifies key m1A-modified genes potentially linking periodontitis and diabetes. The combination of bioinformatics analysis and experimental validation highlights their potential as diagnostic biomarkers and provides novel insights into shared molecular mechanisms of these comorbid conditions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106428"},"PeriodicalIF":2.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucose as a biomarker in oral potentially malignant disorders and oral squamous cell carcinoma: A systematic review and meta-analysis","authors":"Shruti Gupta ,&nbsp;Deepti Sharma ,&nbsp;Anita Hooda ,&nbsp;Mala Kamboj","doi":"10.1016/j.archoralbio.2025.106423","DOIUrl":"10.1016/j.archoralbio.2025.106423","url":null,"abstract":"<div><h3>Objectives</h3><div>This systematic review and meta-analysis aimed to investigate the potential of fucose as a biomarker for oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC).</div></div><div><h3>Design</h3><div>Relevant articles were searched across multiple databases, including PubMed, Scopus, Google Scholar, Science Direct, and Clinical Key. Twenty-three studies that met the eligibility criteria were included for qualitative review; out of which, 20 studies that provided data for comparison between study and control groups were further subjected to meta-analysis.</div></div><div><h3>Results</h3><div>The analysis revealed significantly high fucose levels in the saliva of OPMD patients (p = 0.013) and OSCC patients (p = 0.003) compared to healthy controls. Additionally, fucose levels were elevated in the serum of patients with OSCC (p &lt; 0.001) and oral leukoplakia (p = 0.027) compared to controls.</div></div><div><h3>Conclusion</h3><div>The findings of this meta-analysis suggest that fucose could serve as a potential biomarker for OPMDs and OSCC. However, the presence of significant heterogeneity in serum evaluation and the limited studies focusing on saliva indicate the need for further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106423"},"PeriodicalIF":2.1,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tooth loss induces cognitive decline independent of low-protein diet intake in male mice","authors":"Rie Hatakeyama ,&nbsp;Hiroshi Oue ,&nbsp;Miyuki Yokoi ,&nbsp;Eri Ishida ,&nbsp;Takayasu Kubo ,&nbsp;Kazuhiro Tsuga","doi":"10.1016/j.archoralbio.2025.106421","DOIUrl":"10.1016/j.archoralbio.2025.106421","url":null,"abstract":"<div><h3>Objectives</h3><div>Previous articles have shown that tooth loss is associated with cognitive decline in animal and human studies. Additionally, poor nutritional status including low-protein diet is associated with dementia. The evidence of the association of tooth loss and nutritional status is uncertain. The purpose of this study was to investigate the relationship between low-protein diet intake and cognitive decline following tooth loss in mice.</div></div><div><h3>Design</h3><div>Male senescence-accelerated mouse-prone 8 mice were randomly allocated into sham-operated control and tooth loss groups with extracted maxillary molars: control with normal-protein diet; control with low-protein diet; bilateral maxillary molar extraction with normal-protein diet; and bilateral maxillary molar extraction with low-protein diet. After 6 months, a behavioral test was conducted, and mRNA expression and immunohistochemistry in the brain were analyzed.</div></div><div><h3>Results</h3><div>Behavioral test revealed no effect of the interaction between tooth loss and low-protein diet intake on cognitive decline; however, tooth loss had a marked effect on cognitive decline. Real-time polymerase chain reaction showed no interaction between tooth loss and low-protein intake for <em>Bax/Bcl-2</em> mRNA expression; however, tooth loss had a significant effect on <em>Bax/Bcl-2</em> mRNA expression. Immunohistochemical analysis showed that the effect of tooth loss in neuronal inflammation and neuronal loss were observed in CA1 and DG region, but the effect of low-protein diet was limited in CA3.</div></div><div><h3>Conclusion</h3><div>The present study revealed that the impact of tooth loss on cognitive decline was not dependent on the low-protein diet condition.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106421"},"PeriodicalIF":2.1,"publicationDate":"2025-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B. lactis HN019 modulates periapical inflammation and enhances cementum repair by increasing CEMP-1 expression in an animal model","authors":"Lisa Danielly Curcino Araujo ,&nbsp;Ana Paula Gomes-Moura ,&nbsp;Raquel Assed Bezerra da Silva ,&nbsp;Michel Reis Messora ,&nbsp;Flávia Aparecida Chaves Furlaneto ,&nbsp;Sérgio Luis de Souza Salvador ,&nbsp;Allan Radaic ,&nbsp;Pachiyappan Kamarajan ,&nbsp;Yvonne L. Kapila ,&nbsp;Paulo Nelson-Fillho ,&nbsp;Higinio Arzate ,&nbsp;Samin Sirous ,&nbsp;Geeta Bhuvanagiri ,&nbsp;Léa Assed Bezerra da Silva","doi":"10.1016/j.archoralbio.2025.106420","DOIUrl":"10.1016/j.archoralbio.2025.106420","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of the HN019 probiotic on the modulation of inflammation and the resorption of mineralized tissues in experimentally induced periapical lesions in an animal model, evaluating its impact on the expression of cementum protein 1 (CEMP-1).</div></div><div><h3>Design</h3><div>Periapical lesion was induced in 45 Wistar rats. The animals were divided into groups according to the irrigating solution used. Root canals were irrigated on days 7 and 14. After 21 days, the animals were euthanized, and mandibles were processed for histological analysis. Descriptive and semi-quantitative evaluations of the inflammatory infiltrate, periodontal ligament, bone and cementum resorption, as well as counts of inflammatory cells were performed using HE staining. Also, to assess cementum formation, tissue sections were stained with an antibody for CEMP-1 and analyzed by immunohistochemistry.</div></div><div><h3>Results</h3><div>In the Infection + Probiotic Group, the inflammatory infiltrate was mixed and diffuse, ranging from mild to severe, with no statistically significant difference compared to the Control Group (p &gt; 0.05). However, in the Infection + Probiotic Group, cementum resorption was significantly lower compared to Control Group (p &lt; 0.001). Moreover, CEMP-1 expression was significantly higher in Infection + Probiotic-Group compared with all other groups (p &lt; 0.0001).</div></div><div><h3>Conclusion</h3><div>The probiotic HN019 significantly increased CEMP-1 expression, indicating cementum formation in periapical lesions in vivo. These findings suggest that <em>B. lactis</em> HN019 may have regenerative potential in clinical scenarios. Furthermore, this strain appears to modulate the inflammatory state of periapical lesions toward a reparative phenotype, characterized by reduced bone resorption. Also, inflammatory infiltrate was like control group.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106420"},"PeriodicalIF":2.1,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mirt2 alleviates LPS-induced inflammation of osteoblasts in alveolar bone destruction","authors":"Yajie Wu ,&nbsp;Zhifei Su ,&nbsp;Bowen Zhang ,&nbsp;Lei Cheng ,&nbsp;Ziyou Wang ,&nbsp;He Yuan ,&nbsp;Jiyao Li","doi":"10.1016/j.archoralbio.2025.106410","DOIUrl":"10.1016/j.archoralbio.2025.106410","url":null,"abstract":"<div><h3>Objectives</h3><div>The long noncoding RNA (lncRNA) myocardial infarction–associated transcript 2 (Mirt2) has been confirmed to affect several inflammatory diseases. This study was conducted to explore the functional mechanism of Mirt2 in inflammatory alveolar bone loss and its possibility of being a therapeutic target.</div></div><div><h3>Design</h3><div>The expression level and potential role of Mirt2 in chronic inflammatory alveolar bone loss in mouse models of periodontitis and periapical periodontitis were investigated using micro-CT and qPCR. The characteristics of Mirt2 were evaluated by FISH, qPCR, ELISA, and alkaline phosphatase staining to confirm its function and mechanism of action in inflammatory response.</div></div><div><h3>Results</h3><div>Mirt2 expression was significantly enriched in inflammatory alveolar bone diseases. Mirt2 expression increased upon LPS stimulation in MC3T3-E1 cells (<em>P &lt; 0.05</em>), located at the cell cytoplasm. Mirt2 knockdown exacerbated the LPS-stimulated inflammatory response in MC3T3-E1 cells, whereas Mirt2 overexpression attenuated this effect and rescued LPS-impaired osteogenic differentiation.</div></div><div><h3>Conclusions</h3><div>lncRNA Mirt2 suggests a potential role in chronic inflammation–related bone loss, providing potential therapeutic target worthy of future investigation for inflammation-related bone loss.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106410"},"PeriodicalIF":2.1,"publicationDate":"2025-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hsa_circ_0007349 upregulates glutamate-ammonia ligase to modulate gingival fibroblast wound healing","authors":"Liang Ren ,&nbsp;Linlin He ,&nbsp;Runyu Zhang ,&nbsp;Xiaoyi Li ,&nbsp;Shimeng Xiao ,&nbsp;Shujuan Guo ,&nbsp;Chengcheng Liu ,&nbsp;Yi Ding","doi":"10.1016/j.archoralbio.2025.106419","DOIUrl":"10.1016/j.archoralbio.2025.106419","url":null,"abstract":"<div><h3>Objectives</h3><div>Human gingival fibroblasts (HGFs) are important cells that maintain the structure and integrity of periodontal tissues. Circular RNAs (circRNAs) play critical roles in cellular processes of periodontitis; however, their roles in HGFs remain unclear. This study aimed to elucidate the function and mechanism of a novel circRNA, hsa_circ_0007349, in periodontitis progression by validating its existence, establishing its role in a ceRNA network (targeting miR-127–5p/GLUL), assessing its regulatory effects on HGF functions in vitro, and confirming its pathological impact in a murine periodontitis model.</div></div><div><h3>Design</h3><div>We performed circRNA sequencing to identify differentially expressed circRNAs between periodontitis and healthy gingival tissues. We selected hsa_circ_0007349, and confirmed its circular structure by Sanger sequencing, RNase R and actinomycin D assays. hsa_circ_0007349/miR-127–5p/glutamate-ammonia ligase (GLUL) interactions were explored using bioinformatics analysis, dual-luciferase reporter assays, RT-qPCR, and western blotting, and their effects on HGF functions were assessed via transcriptome sequencing, Cell Counting Kit-8, cell scratch, and Transwell assays. To further investigate the pathogenic mechanisms, we established a standardized murine periodontitis model using female C57BL/6 mice, followed by histopathological evaluation of periodontal tissue destruction.</div></div><div><h3>Results</h3><div>Hsa_circ_0007349 expression significantly increased in gingival tissues of patients with periodontitis and in HGFs treated with <em>Porphyromonas gingivalis</em> lipopolysaccharide. hsa_circ_0007349 regulates miR-127–5p to affect cell proliferation, migration, and wound healing, and affects extracellular matrix metabolism and periodontal tissue damage via GLUL.</div></div><div><h3>Conclusion</h3><div>Hsa_circ_0007349 acts as a competing endogenous RNA to promote HGF proliferation, migration, wound healing and extracellular matrix turnover by targeting miR-127–5p and GLUL.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106419"},"PeriodicalIF":2.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct oral microbiome profiles in substance users in the high-incidence Mizo tribal community, Northeast India","authors":"Lalfak Zuali ,&nbsp;Lalchhanhima Ralte ,&nbsp;John Zothanzama ,&nbsp;Christine Vanlalbiakdiki Sailo ,&nbsp;Nachimuthu Senthil Kumar","doi":"10.1016/j.archoralbio.2025.106418","DOIUrl":"10.1016/j.archoralbio.2025.106418","url":null,"abstract":"<div><h3>Objectives</h3><div>To characterize the oral microbiome of substance users (primarily tobacco, alcohol, and opioids) compared to healthy controls in the high-incidence Mizo tribal community of Northeast India, and to examine their association with food and lifestyle habits.</div></div><div><h3>Methods</h3><div>Saliva samples from 53 substance users and 35 healthy controls were subjected to 16S rRNA (V3–V4 region) sequencing. Alpha and beta diversity analyses, differential abundance testing, and microbial functional prediction were conducted. Statistical analyses were corrected for multiple comparisons using false discovery rate (FDR) adjustment where applicable. Associations with food and lifestyle habits were also examined.</div></div><div><h3>Results</h3><div>Substance users exhibited significantly lower alpha diversity, and beta diversity analyses revealed distinct clustering between the two groups. Firmicutes_D, Actinobacteria, <em>Rothia</em>, and <em>Streptococcus</em> were more abundant in substance users, whereas Bacteroidota, Proteobacteria, <em>Neisseria</em>, and <em>Prevotella</em> were enriched in healthy controls. Functional predictions indicated upregulation of biofilm formation and xenobiotic degradation pathways in substance users. Exposure to Jhum cultivation, prenatal smoking, and consumption of fermented pork fat (saum) were correlated with microbial composition.</div></div><div><h3>Conclusions</h3><div>Substance use and associated environmental exposures were linked to oral microbiome dysbiosis. Certain bacterial taxa may serve as potential microbial biomarkers of substance use in this high-risk tribal population, warranting further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106418"},"PeriodicalIF":2.1,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}