Archives of oral biology最新文献

{"title":"Enhanced regenerative potential of human dental pulp stem cells for the pulp-dentin complex through coculture with iPSC-derived endothelial cells: An in vitro study","authors":"Hsu Myat Cho ,&nbsp;Ukseong Kim ,&nbsp;Sunil Kim ,&nbsp;Stephanie Myeong Choi ,&nbsp;Sukjoon Lee ,&nbsp;Euiseong Kim","doi":"10.1016/j.archoralbio.2025.106409","DOIUrl":"10.1016/j.archoralbio.2025.106409","url":null,"abstract":"<div><h3>Objectives</h3><div>Although cell-based therapies using human dental pulp stem cells (hDPSCs) with other cell lineages and growth factors show promise in regenerative endodontics, combining hDPSCs with induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) is unexplored. Moreover, iPSC-ECs overcome ethical and practical challenges related to primary endothelial cells. This study explored the odontogenic and angiogenic potential of hDPSCs and iPSC-ECs in direct coculture.</div></div><div><h3>Design</h3><div>hDPSCs were isolated from extracted human teeth, and iPSC‑ECs were generated via episomal reprogramming of hDPSCs followed by endothelial differentiation. Four groups were established for differentiation assays: hDPSCs in basal medium, osteogenic medium, modified osteogenic medium (D‑MOD), and coculture with iPSC‑ECs (1:5) in D‑MOD. Mineralization was assessed by alkaline phosphatase and alizarin red S staining; gene expression of odontogenic (<em>DSPP, IBSP, ALPL</em>) and angiogenic (<em>PECAM1, MCAM, KDR</em>) markers was measured by RT‑qPCR; protein levels were evaluated by Western blot and nestin immunofluorescence; and angiogenic capacity in the D‑MOD and coculture groups was quantified via Matrigel tube‑formation assay.</div></div><div><h3>Results</h3><div>The coculture group showed enhanced mineralization and significantly increased expression of <em>DSPP</em>, <em>IBSP</em>, and <em>PECAM1</em>. Protein analysis confirmed elevated DSPP and nestin levels. Tube formation assays revealed significantly more junctions, segments, and meshes in the coculture group.</div></div><div><h3>Conclusions</h3><div>This study demonstrated <em>in vitro</em> that coculturing hDPSCs with iPSC-ECs enhances both odontogenic and angiogenic differentiation compared to hDPSCs cultured alone. These findings highlight the potential of iPSC technology in regenerative endodontics and indicate a promising cell-based approach for future therapeutic applications.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106409"},"PeriodicalIF":2.1,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological traits and caries susceptibility of the cusp of carabelli in permanent maxillary molars: A study in Lahore, Pakistan","authors":"Maliha Shahbaz ,&nbsp;Naauman Zaheer ,&nbsp;Usman Zaheer ,&nbsp;Madiha ,&nbsp;Muhammad Haris Bilal ,&nbsp;Abdullah Sajid ,&nbsp;Junaid Ali ,&nbsp;Hareem Aziz ,&nbsp;Khurram Nadeem","doi":"10.1016/j.archoralbio.2025.106406","DOIUrl":"10.1016/j.archoralbio.2025.106406","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the prevalence, morphological traits, and caries susceptibility of the Cusp of Carabelli (CoC) in permanent maxillary molars among patients in Lahore, Pakistan.</div></div><div><h3>Design</h3><div>A descriptive cross-sectional study was conducted among 432 participants aged 12 years or older at Lahore Medical and Dental College. Clinical examination of maxillary first and second molars was performed using the Arizona State University Dental Anthropology System (ASUDAS) for CoC traits and the International Caries Detection and Assessment System (ICDAS) for caries assessment. Inter- and intra-examiner calibration ensured diagnostic reliability (Cohen's Kappa &gt;0.90).</div></div><div><h3>Results</h3><div>CoC (ASUDAS grades 1–7) was observed in 201 individuals (46.5 %) on maxillary first molars and in 7 individuals (1.6 %) on second molars, with bilateral expression more common than unilateral. The right first molar showed a higher prevalence of CoC and caries incidence. Morphological traits ranged from subtle grooves to pronounced cusps, with small vertical grooves (ASUDAS 1) being the most frequent. Caries susceptibility correlated positively with CoC prominence (p &lt; 0.001). Multivariate logistic regression identified CoC grade as the strongest predictor of caries, overshadowing age, side, and molar position, and substantially improving model sensitivity (0 % to 97.2 %).</div></div><div><h3>Conclusion</h3><div>CoC is a prevalent trait and is significantly associated with early-stage dental caries in maxillary first molars. Its presence, particularly in prominent forms, may pose an increased risk of caries. These findings underscore the need for enhanced preventive strategies and clinical attention in individuals with CoC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106406"},"PeriodicalIF":2.1,"publicationDate":"2025-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endoplasmic reticulum stress as a nexus of temporomandibular joint osteoarthritis","authors":"Xinqi Huang ,&nbsp;Zinan Cen ,&nbsp;Xinxuan Zhou ,&nbsp;Zhihe Zhao ,&nbsp;Xiao Cen","doi":"10.1016/j.archoralbio.2025.106407","DOIUrl":"10.1016/j.archoralbio.2025.106407","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to summarize the molecular architecture of endoplasmic reticulum (ER) stress signaling networks and their mechanistic involvement in temporomandibular joint osteoarthritis (TMJOA) progression, and current therapeutic strategies targeting ER stress mediators and the obstacles from bench to bedside.</div></div><div><h3>Design</h3><div>The related literatures of the roles of ER in TMJOA were searched through PubMed database by different combinations of the following keywords including animal models, ER, unfolded protein response (UPR), ER-associated degradation (ERAD), ER-phagy, TMJ, and OA. No filters were used in the search. The references of eligible studies were also analyzed and reviewed comprehensively.</div></div><div><h3>Results</h3><div>This review discussed how ER stress signaling orchestrated TMJOA pathogenesis, including UPR, ERAD, and ER-phagy. It was also summarized how biomechanical stress and hypoxic microenvironment synergistically exacerbated ER stress, and the current therapeutic strategies for TMJOA based on ER stress modulators and the obstacles in bench-to-bedside research.</div></div><div><h3>Conclusions</h3><div>ER proteostasis represented a pivotal but underexplored therapeutic axis in TMJOA. Bridging the gap between mechanistic understanding of ER stress adaptation and TMJ-specific pathobiology is essential for developing novel therapeutic strategies for TMJOA.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106407"},"PeriodicalIF":2.1,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145202279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial effects of charcoal and fluoride dentifrices in oral biofilms","authors":"Ashley N. Bowers ,&nbsp;Caroline Coradi Tonon ,&nbsp;Sam Yeo ,&nbsp;Kinga Vojnits ,&nbsp;Rayhan Shah ,&nbsp;Sepideh Pakpour ,&nbsp;Simone Duarte","doi":"10.1016/j.archoralbio.2025.106405","DOIUrl":"10.1016/j.archoralbio.2025.106405","url":null,"abstract":"<div><h3>Objectives</h3><div>Charcoal-containing dentifrices are increasingly popular for their whitening claims, but data on antimicrobial effects are limited. This study compared the antibacterial efficacy of charcoal dentifrices versus non-charcoal dentifrices containing sodium fluoride (NaF), stannous fluoride (SnF₂), or sodium monofluorophosphate (NaMFP) against multi-species oral biofilms.</div></div><div><h3>Methods</h3><div>Biofilms of <em>Streptococcus mutans</em>, <em>S. gordonii</em>, and <em>S. sanguinis</em> were grown on hydroxyapatite discs and treated for 60 s with 6 dentifrice slurries (3 charcoal, 3 non-charcoal dentifrices) or controls (saline and 0.12 % chlorhexidine, CHX). Antibacterial effects were assessed by CFU/mL; (n = 9/group) and qPCR (n = 3/group). For fluoride-type analyses, charcoal and non-charcoal dentifrices were combined (CFU n = 18/type; qPCR n = 6/type). Percent reduction was compared across groups using one-way ANOVA with post-hoc tests.</div></div><div><h3>Results</h3><div>NaF dentifrices exhibited the greatest overall antibacterial activity (46.8 % reduction), followed by NaMFP (34.9 %), while SnF₂ showed minimal effect (≤ 5.7 %). Charcoal inclusion did not enhance efficacy and slightly reduced NaF activity. Species-specific responses varied: NaF eliminated <em>S. gordonii</em>, and significantly reduced <em>S. mutans</em> and <em>S. sanguinis</em>. Charcoal inclusion did not significantly alter species-level viability. qPCR supported CFU trends but showed limited between-group differences. Overall, fluoride type – not charcoal – primarily determined efficacy (NaF &gt; NaMFP &gt; SnF₂).</div></div><div><h3>Conclusions</h3><div>Fluoride type had a greater impact on antibacterial efficacy than charcoal. NaF was most effective, while SnF₂ least. Charcoal offered no benefit and may slightly diminish NaF performance. Fluoride choice is more critical than charcoal additives for caries prevention.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106405"},"PeriodicalIF":2.1,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OIP5-AS1 expression profiles in different stages of oral squamous cell carcinoma","authors":"Ameya K.P.,&nbsp;Ashikha Shirin Usman P.P.,&nbsp;Durairaj Sekar","doi":"10.1016/j.archoralbio.2025.106403","DOIUrl":"10.1016/j.archoralbio.2025.106403","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the expression patterns of long non-coding RNA OIP5-AS1 across various stages of oral squamous cell carcinoma (OSCC) and assess its potential as a biomarker and therapeutic target.</div></div><div><h3>Design</h3><div>A comprehensive review of recent literature focusing on OIP5-AS1's role in OSCC was conducted. Analysis included OIP5-AS1 expression levels in cancerous versus non-cancerous tissues and exploration of its interactions with tumor-suppressing microRNAs (miRNAs).</div></div><div><h3>Results</h3><div>OIP5-AS1 was found to be significantly upregulated in advanced stages of OSCC compared to non-cancerous tissues. Its function as a molecular sponge for miRNAs contributes to the promotion of tumorigenic pathways, complicating therapeutic responses and highlighting its role as an oncogene.</div></div><div><h3>Conclusions</h3><div>OIP5-AS1 is a critical player in the progression of OSCC, influencing tumor dynamics and mechanisms of resistance. Elucidating its expression patterns and functional roles suggests that OIP5-AS1 could serve as a valuable biomarker for early diagnosis and personalized treatment strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106403"},"PeriodicalIF":2.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression patterns of desmosome family members during tooth development and the role of Desmocollin-3 in cytodifferentiation of stratum intermedium","authors":"Triana Marchelina ,&nbsp;Yuta Chiba ,&nbsp;Shinji Otake ,&nbsp;Li Wanshu ,&nbsp;Hiroshi Sato ,&nbsp;Yumiko Nakashima ,&nbsp;Asuna Sugimoto ,&nbsp;Tsutomu Iwamoto ,&nbsp;Aya Yamada ,&nbsp;Kan Saito ,&nbsp;Satoshi Fukumoto","doi":"10.1016/j.archoralbio.2025.106404","DOIUrl":"10.1016/j.archoralbio.2025.106404","url":null,"abstract":"<div><h3>Objective</h3><div>Desmocollin-3 (Dsc3), a desmosomal cadherin, is critical in maintaining epithelial cohesion and integrity. Despite its recognized function in skin and mucosal epithelium, its contribution to tooth development remains poorly understood. The study aims to identify stratum intermedium (SI)-specific markers using single-cell RNA-sequence (scRNA-seq) and to investigate the functional role of Dsc3 in maintaining SI cell integrity and differentiation.</div></div><div><h3>Design</h3><div>In this study, we pursued the marker genes of SI cells using scRNA-seq analysis of post-natal day 12 molar. Furthermore, we examined the role of the SI marker gene using dental epithelial cell line SF2.</div></div><div><h3>Results</h3><div>We found that desmosome family genes are highly expressed in SI cluster and among them, Dsc3 showed specific expression in SI cluster. Knockdown of Dsc3 in the SF2 epithelial cell line led to significantly smaller cell size, indicating impaired epithelial differentiation. The expression of SI marker genes was suppressed by the knockdown of Dsc3 with a marked loss of tight junction protein Tjp1 (ZO-1), indicating disrupted intercellular junctions and impaired epithelial barrier function. This disruption correlated with altered expression of key ameloblast differentiation markers, suggesting a failure in proper ameloblast lineage commitment, highlighting a disruption in the SI’s ability to support ameloblast lineage specification.</div></div><div><h3>Conclusion</h3><div>These findings indicate that Dsc3 is essential for SI structural integrity and its signaling support to ameloblasts.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106404"},"PeriodicalIF":2.1,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro antibacterial activity of Lactiplantibacillus sp. cell-free supernatants against Porphyromonas gingivalis: A potential approach for oral health","authors":"Hui Ying Yit ,&nbsp;Zaleha Shafiei ,&nbsp;Noraziah Mohamad Zin ,&nbsp;Mazlina Mohd Said","doi":"10.1016/j.archoralbio.2025.106399","DOIUrl":"10.1016/j.archoralbio.2025.106399","url":null,"abstract":"<div><h3>Objective</h3><div>This study explores the potential of postbiotic metabolites from lactic acid bacteria (LAB) isolated from human milk and tempeh against <em>Porphyromonas gingivalis</em>, a key pathogen in periodontitis.</div></div><div><h3>Design</h3><div>Selected LAB strains and their treated cell-free supernatants (TCFS), lyophilized TCFS (L-TCFS), and bacteriocin-like inhibitory substances (BLIS) were tested individually and in combination using agar well diffusion, MIC, MBC, autoaggregation, coaggregation, and biofilm inhibition assays. LAB identification was performed using the API 50 CHL kit and 16S rDNA sequencing.</div></div><div><h3>Results</h3><div>Strain S2, a mixture of <em>Lactiplantibacillus</em> sp. SUK1 and T2 showed the highest inhibitory activity (20.06 ± 4.14 mm) using the agar well diffusion method. It demonstrated strong autoaggregation compared to the individual T2 strain but lacked significant coaggregation ability. The L-TCFS of S2 also exhibited the strongest antibacterial effect, with a minimum inhibitory concentration (MIC) of 50 mg/mL, although no minimum bactericidal concentration (MBC) was detected for any bacteriocin-like inhibitory substances (BLIS). L-TCFS from strain S2 significantly reduced <em>P. gingi</em>valis biofilm formation at a minimum concentration of 25 mg/mL, compared to the untreated control. Strain T2 was identified as <em>Lactiplantibacillus plantarum</em> using the API 50CHL test and 16S rDNA gene sequencing.</div></div><div><h3>Conclusion</h3><div>The combination metabolite S2 demonstrates promising inhibitory activity against <em>P. gingivalis</em> and may serve as an effective adjunctive therapy for periodontal infections, outperforming individual and other combination LAB strains tested.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106399"},"PeriodicalIF":2.1,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and thermal signatures of dental tissues in third molars: An in vitro comparative study using fourier-transform infrared spectroscopy and differential scanning calorimetry","authors":"Rola Zahedah ,&nbsp;Recep Üstünsoy ,&nbsp;Aliye Tuğçe Gürcan ,&nbsp;Bircan Dinç","doi":"10.1016/j.archoralbio.2025.106402","DOIUrl":"10.1016/j.archoralbio.2025.106402","url":null,"abstract":"<div><h3>Objective</h3><div>To characterize and compare the molecular and thermal characteristics of enamel, dentin, cementum, and the dentin–pulp complex in permanent third molars using Fourier-transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC).</div></div><div><h3>Design</h3><div>Samples from extracted third molars (n = 15) were prepared and analyzed using FTIR to assess molecular composition and DSC to evaluate thermal transitions, including dehydration, collagen degradation, and mineral phase transformation. All measurements were conducted in triplicate.</div></div><div><h3>Results</h3><div>FTIR revealed enamel as highly mineralized with minimal organic content, dentin and cementum as collagen-rich, and the dentin–pulp complex as a hybrid tissue. DSC analysis identified consistent thermal transitions: water loss (110–125 °C), collagen breakdown (300–320 °C), and mineral decomposition (455–470 °C). Enamel displayed the highest crystallinity, while cementum exhibited the highest enthalpy change. Tissues with stronger FTIR collagen peaks corresponded to higher DSC energy release during protein degradation.</div></div><div><h3>Conclusion</h3><div>Molecular and thermal profiling of dental tissues provide baseline reference data for biomaterial design and regenerative strategies.</div></div><div><h3>Clinical significance</h3><div>Understanding tissue-specific molecular and thermal properties can guide the development of biomimetic restorative materials, inform safer thermal thresholds during clinical procedures, and support diagnostic approaches for aging and pathological changes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106402"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZBP1-NLRP3 axis integrates PANoptosis and ferroptosis during inflammatory injury in human dental pulp fibroblasts","authors":"Ai-E. He ,&nbsp;Xing Wang ,&nbsp;Ni Xie,&nbsp;Yun-He Xiao","doi":"10.1016/j.archoralbio.2025.106398","DOIUrl":"10.1016/j.archoralbio.2025.106398","url":null,"abstract":"<div><h3>Objective</h3><div>To define how Z-DNA binding protein 1 (ZBP1) and NOD-like receptor family pyrin domain-containing 3 (NLRP3) signaling regulate lipopolysaccharide (LPS)-induced inflammation, PANoptosis, and ferroptosis in human dental pulp fibroblasts (HDPFs).</div></div><div><h3>Design</h3><div>HDPFs were treated with LPS, and <em>ZBP1</em> and <em>NLRP3</em> were silenced using small interfering RNA (siRNA), individually or in combination. Inflammatory mediators and death-pathway markers were quantified by quantitative real-time PCR (qRT-PCR), Western blotting, enzyme-linked immunosorbent assay (ELISA), and biochemical assays; Annexin V/propidium iodide flow cytometry assessed cell-death distributions.</div></div><div><h3>Results：</h3><div>LPS significantly increased ZBP1 and NLRP3 expression and elevated cytokine/chemokine release; each was attenuated by <em>ZBP1</em> or <em>NLRP3</em> knockdown, with the greatest reduction after dual silencing. LPS triggered PANoptosis, as indicated by increased Annexin V⁺/PI⁺ cell populations and upregulation of caspase-1, cleaved caspase-8, RIPK3, GSDMD, and p-MLKL/MLKL, which were significantly reduced by inhibition of the ZBP1-NLRP3 axis. Ferroptosis features were also evident after LPS, including impaired iron homeostasis (downregulated ferritin heavy chain 1 [FTH1] and ferroportin [FPN1] with Fe²⁺ accumulation), enhanced lipid peroxidation (upregulated ALOX15, LPCAT3, PTGS2 with increased malondialdehyde and lipid reactive oxygen species), and weakened antioxidant defenses (reduced glutathione peroxidase-4 [GPX4], solute carrier family 7 member 11 [SLC7A11], glutathione, and GPX4 activity). These changes were mitigated by single-gene silencing and most effectively by dual knockdown.</div></div><div><h3>Conclusion</h3><div>The ZBP1-NLRP3 axis acts upstream to coordinate LPS-induced PANoptosis and ferroptosis in HDPFs. Targeting this axis dampens inflammatory cell death and oxidative-metabolic dysregulation, highlighting a potential therapeutic strategy for pulpitis-related tissue injury.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106398"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical assessment of murine double minute 2 in solid vs unicystic ameloblastoma: A systematic review and meta-analysis","authors":"Lilibeth-Stephania Escoto-Vasquez ,&nbsp;Mario Alberto Alarcón-Sánchez ,&nbsp;Julieta Sarai Becerra-Ruiz ,&nbsp;Cristina Hermila Martínez-Bugarin ,&nbsp;Sarah Monserrat Lomelí-Martínez ,&nbsp;Armen A. Muradyan ,&nbsp;Artak Heboyan","doi":"10.1016/j.archoralbio.2025.106400","DOIUrl":"10.1016/j.archoralbio.2025.106400","url":null,"abstract":"<div><h3>Objective</h3><div>This project aimed to evaluate the immunoexpression pattern of murine double minute 2 (MDM2) in solid ameloblastomas compared to unicystic ameloblastomas.</div></div><div><h3>Methods</h3><div>The review followed PRISMA guidelines and was registered in the PROSPERO database. PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar were comprehensively searched. Original cross-sectional studies were included. The meta-analysis was performed using STATA V15 and RevMan. Positivity rates were pooled using a random-effects model (REM), the labeling index was analyzed using mean difference under a REM, and expression intensity (moderate–strong) was assessed as categorical data using a REM with Hartung-Knapp adjustment. Heterogeneity was evaluated with the Chi² test and I² statistic. The methodological quality and certainty of the evidence were assessed using the Joanna Briggs Institute items and the GRADE system.</div></div><div><h3>Results</h3><div>Nine studies (n = 438 specimens) were analyzed, of which 325/438 (74.2 %) were ameloblastoma biopsies. MDM2 positivity was detected in 403/438 cases (92 %). A statistically significant association in favor of solid ameloblastoma was observed (RR = 2.08; 95 %CI [1.66–2.60]; p = 0.005), indicating a twofold probability of finding high MDM2 expression in solid compared to unicystic ameloblastomas. Four of the nine studies (44.4 %) were considered to be of low quality, and the certainty of evidence was low to very low.</div></div><div><h3>Conclusions</h3><div>MDM2 expression was prevalent in both types of ameloblastomas, with a higher intensity of expression observed in solid cases. However, due to study heterogeneity, further investigations with more robust methodological designs are recommended to assess the diagnostic potential of MDM2 in ameloblastomas.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106400"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}