Archives of oral biology最新文献

{"title":"Protective effect of bisabolol on glucose metabolism in salivary gland of male diabetic rats","authors":"Letícia Martins Santos ,&nbsp;Carla Renata Sipert ,&nbsp;Gabriel Soares dos Santos ,&nbsp;Gabriella Soares dos Santos ,&nbsp;Thiago José Dionísio ,&nbsp;Carlos Ferreira dos Santos ,&nbsp;Douglas Nesadal de Souza ,&nbsp;Fernando Neves Nogueira","doi":"10.1016/j.archoralbio.2025.106246","DOIUrl":"10.1016/j.archoralbio.2025.106246","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the effect of bisabolol treatment on the activity and expression of the main enzymes of the glycolytic pathway in the submandibular and parotid salivary glands of Streptozotocin (STZ)-induced diabetic rats.</div></div><div><h3>Design</h3><div>Twenty-four male Wistar rats, aged 60 days, were divided into four groups: Control, diabetic, control+Bisabolol, and diabetic+Bisabolol. A single intraperitoneal injection of Streptozotocin induced diabetes. The treated group received a daily dose of α-Bisabolol (25 mg/kg of body weight dissolved in 0.1 mL of sunflower oil) by gavage for 21 days. The untreated group received only the vehicle. All animals were euthanized 28 days after induction, and the parotid and submandibular glands were removed, frozen in liquid nitrogen, and stored at −80 °C. The expression of the main enzymes of the glycolytic pathway was analyzed through RT-qPCR. Biochemical analyses were conducted to verify Hexokinase (HK), Phosphofructokinase (PFK), Pyruvate kinase (PK), and Lactate dehydrogenase (LDH) activity. The data were statistically compared via the Student t-test and analysis of variance (ANOVA) followed by Tukey’s test, where results were considered statistically significant at p &lt; 0.05.</div></div><div><h3>Results</h3><div>In general, bisabolol restored the HK and PFK activities and also restored Hexokinase 2 (<em>Hk2</em>), Phosphofructokinase muscle isoform (<em>Pfkm</em>) and Phosphofructokinase platelet isoform (<em>Pfkp</em>) expression in the bisabolol-treated diabetic rats. The LDH activity increased in diabetic rats with bisabolol.</div></div><div><h3>Conclusion</h3><div>Bisabolol treatment restored the activity and expression of key glycolytic enzymes, suggesting that it may have therapeutic potential in mitigating the salivary glands metabolic dysregulation caused by diabetes in rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106246"},"PeriodicalIF":2.2,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The pan-BH-3 mimetic, obatoclax, synergistically enhances cisplatin-induced apoptosis in oral squamous cell carcinoma through a mechanism that involves degradation of the pro-survival protein Mcl-1","authors":"Tianyi Gao,&nbsp;Stefania Magnano,&nbsp;Molly Quadros,&nbsp;Patricia Hannon Barroeta,&nbsp;Daniela M. Zisterer","doi":"10.1016/j.archoralbio.2025.106250","DOIUrl":"10.1016/j.archoralbio.2025.106250","url":null,"abstract":"<div><h3>Objectives</h3><div>The purpose of the study was to elucidate the role of the Bcl-2 family of proteins in mediating cisplatin resistance in oral squamous cell carcinoma (OSCC). The value of the BH3-mimetics venetoclax and obatoclax as sensitisers for cisplatin treatment in OSCC was also evaluated.</div></div><div><h3>Design</h3><div>In this study the expression levels of a series of pro- and anti-apoptotic members of the Bcl-2 family in paired cisplatin-sensitive (SCC4) and resistant (SCC4cisR) tongue squamous carcinoma cell lines were examined by western blotting. The apoptotic rate induced by cisplatin and BH3-mimetics venetoclax and obatoclax alone or in combination in OSCC was also evaluated by Annexin V/Propidium Iodide double-stained flow cytometric assays.</div></div><div><h3>Results</h3><div>Obatoclax was shown to synergistically enhance cisplatin-induced apoptosis, and this enhancement was associated with a marked degradation in pro-survival Mcl-1 and upregulation in conformationally active form of pro-apoptotic Bak.</div></div><div><h3>Conclusions</h3><div>Our study presents novel insights into the relationship between the Bcl-2 family and cisplatin efficacy in OSCC. It also demonstrates that targeted therapy with BH-3 mimetics, such as obatoclax, may represent a new strategy for OSCC therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106250"},"PeriodicalIF":2.2,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bevacizumab and cytostatics induce oxidative changes in the submandibular gland of male rats","authors":"Dubersarsky Claudio Gastón ,&nbsp;Bachmeier Evelin ,&nbsp;Porta Daniela Josefina ,&nbsp;Moine Lorena ,&nbsp;Francia Catalina Melchora ,&nbsp;Rivoira María Angélica ,&nbsp;Mazzeo Marcelo Adrián","doi":"10.1016/j.archoralbio.2025.106248","DOIUrl":"10.1016/j.archoralbio.2025.106248","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate whether the administration of bevacizumab in combination with cytostatics could cause an oxidative response in the submandibular gland of an animal model.</div></div><div><h3>Materials and methods</h3><div>48 Male Wistar rats (350–400 g) were used. <strong>Group 1:</strong> Control; <strong>Group 2:</strong> 5-Flourouracil + Leucovorin calcium (Intraperitoneal injection for five consecutive days with 20 mg/kg+10 mg/kg); <strong>Group 3</strong>: Bevacizumab, two intraperitoneal doses of 0.2 mg/kg on days 1 and 15; <strong>Group 4:</strong> Oxaliplatin, one intraperitoneal dose of 25 mg/kg on days 1 and 15; <strong>Group 5:</strong> Bevacizumab+Oxaliplatin+ 5-Fluorouracil+Leucovorin calcium, single intraperitoneal dose of 20 mg/kg, 10 mg/kg, 0.2 mg/kg, and 25 mg/kg on days 1 and 15; <strong>Group 6:</strong> pair-fed group without drugs. In submandibular gland homogenates, Uric Acid, Lipid Peroxides, Aqueous Peroxides, and Superoxide Dismutase activity were measured. <strong>Results:</strong> Uric Acid in Groups 1, 3, and 6 were higher than in cytostatic groups (p &lt; 0.02, 0.02, and 0.001). Lipid Peroxides and Aqueus Peroxides were similar. Superoxide Dismutase Activity was higher in Groups 2, 3, 4, 5, and 6 compared to Group 1 (p &lt; 0.0001). In Group 3, Superoxide Dismutase Activity was lower than in cytostatic-treated groups but higher than Group 6 (p &lt; 0.001). Group 2 showed higher activity compared to Groups 3 and 6 (p &lt; 0.0001).</div></div><div><h3>Conclusions</h3><div>Cytostatic treatment exerted a pro-oxidant effect at the acinar level. Conversely, bevacizumab may promote an antioxidant glandular response. Further research into these treatments' effects on the stomatognathic system is crucial for improving quality of life in patients undergoing anti-tumour therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106248"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143817078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroethanolic extract of Spondias mombin L. leaves attenuates alveolar bone loss and inflammation in a model of periodontitis induced in male Wistar rats","authors":"Ana Beatriz Costa Almeida ,&nbsp;Agnes Andrade Martins ,&nbsp;Susana Barbosa Ribeiro ,&nbsp;Valkleidson Santos de Araújo ,&nbsp;Heloísa Espínola S. Costa ,&nbsp;Maria Luiza Diniz de Sousa Lopes ,&nbsp;Caroline Addison Carvalho Xavier de Medeiros ,&nbsp;Ericka Janine Dantas Silveira ,&nbsp;Basílio Rodrigues Vieira ,&nbsp;Frederico Barbosa Sousa ,&nbsp;Luciana Ellen Dantas Costa ,&nbsp;Silvana Maria Zucolotto ,&nbsp;Bárbara Cabral ,&nbsp;Maria Raquel Cavalcanti Inácio ,&nbsp;Bruno Cesar de Vasconcelos Gurgel ,&nbsp;Raimundo Fernandes de Araújo Júnior ,&nbsp;Emily Lima Oliveira ,&nbsp;Aurigena Antunes de Araújo ,&nbsp;Ruthineia Diógenes Alves Uchoa Lins","doi":"10.1016/j.archoralbio.2025.106249","DOIUrl":"10.1016/j.archoralbio.2025.106249","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the anti-inflammatory and antiresorptive effects of hydroethanolic extract of <em>Spondias mombin</em> L. in an experimental model of periodontitis.</div></div><div><h3>Design</h3><div>Sixty-three male Wistar rats were divided into five groups: 1) Control, 2) Periodontitis, 3) Periodontitis + <em>Spondias mombin</em> L. 50 mg/kg, 4) Periodontitis + <em>Spondias mombin</em> L. 100 mg/kg, 5) Periodontitis + <em>Spondias mombin</em> L. 200 mg/kg. Periodontitis was induced through the placement of a ligature in the cervical region of the left maxillary second molar. The rats received the extract by oral gavage for 10 days and euthanasia was performed on the 11th day of the experiment. Blood samples were used for biochemical analysis, hemi-maxillae for micro-computed tomography and histological evaluation, and gingival tissue for cytokine and RT-qPCR analyses.</div></div><div><h3>Results</h3><div>Administration of the extract reduced linear alveolar bone loss at a dose of 100 mg/kg (<em>p</em> = 0.0160) and improved bone volumetric parameters, such as integrity (BV/TV%) and trabecular porosity, at doses of 100 mg/kg (<em>p</em> = 0.0180) and 200 mg/kg (<em>p</em> = 0.0117). Trabecular separation was also improved at a dose of 200 mg/kg (<em>p</em> = 0.0405). Rats treated with 200 mg/kg showed better histological parameters (<em>p</em> = 0.0274). There was a reduction in the dosage of the pro-inflammatory cytokine interleukin 1β at doses of 50 mg/kg (<em>p</em> = 0.0496) and 100 mg/kg (<em>p</em> = 0.0374).</div></div><div><h3>Conclusions</h3><div>Our findings suggest an anti-inflammatory and antiresorptive effect of the hydroethanolic extract of <em>Spondias mombin</em> L. administered to rats with periodontitis, without altering blood biochemical markers.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106249"},"PeriodicalIF":2.2,"publicationDate":"2025-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143785706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antifungal and antibiofilm activity of Lippia microphylla Cham. essential oil. (Verbenaceae) on Candida albicans and human cell cytotoxicity","authors":"Jaqueline Oliveira Barreto ,&nbsp;Larissa Alves da Silva ,&nbsp;Luanna de Oliveira e Lima ,&nbsp;Paula Lima Nogueira ,&nbsp;Rafael Carlos Ferreira ,&nbsp;Heivila Monique da Silva Alexandre ,&nbsp;Anauara Lima e Silva ,&nbsp;Juan Carlos Ramos Gonçalves ,&nbsp;Marianna Vieira Sobral ,&nbsp;Ricardo Dias de Castro ,&nbsp;Yuri Mangueira do Nascimento ,&nbsp;Josean Fechine Tavares ,&nbsp;Walicyranison Plinio da Silva Rocha ,&nbsp;Felipe Queiroga Sarmento Guerra","doi":"10.1016/j.archoralbio.2025.106243","DOIUrl":"10.1016/j.archoralbio.2025.106243","url":null,"abstract":"<div><h3>Objective</h3><div>This study evaluated the antifungal activity of <em>Lippia microphylla</em> essential oil (LM-EO) on <em>Candida albicans</em>.</div></div><div><h3>Design</h3><div>In vitro assays were conducted to test LM-EO for its anti-Candida effects, antibiofilm activity, effects on the fluorescence intensity of <em>Candida albicans</em> biofilms observed via confocal microscopy, probable mechanism of action, and toxicity on human keratinocytes. Statistical analysis was performed considering α = 5 %.</div></div><div><h3>Results</h3><div>The minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC) of LM-EO ranged from 128 µg/mL to 256 µg/mL and 256 µg/mL to 1024 µg/mL, respectively. MIC values did not change in the presence of sorbitol, whereas a 4-fold increase was observed in the presence of ergosterol, suggesting that LM-EO may act on the cell membrane. This was subsequently observed via confocal microscopy using propidium iodide markers. LM-EO reduced biofilm adherence by 66–86 % (p &lt; 0.0001) at low concentrations (256–2560 µg/mL); however, it showed toxicity in human cells.</div></div><div><h3>Conclusions</h3><div>LM-EO exhibited fungicidal activity likely through a mechanism related to ergosterol complexation but demonstrated cytotoxicity to human keratinocytes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106243"},"PeriodicalIF":2.2,"publicationDate":"2025-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colorimetric and visual assessment of two-color chewing gum mixing ability test: Correlation and reference parameters in dentate subjects","authors":"Murali Srinivasan ,&nbsp;Lisa Takeshita ,&nbsp;Prachi Jain ,&nbsp;Yasmin Milhomens Moreira ,&nbsp;Martin Schimmel ,&nbsp;Claudio R. Leles","doi":"10.1016/j.archoralbio.2025.106245","DOIUrl":"10.1016/j.archoralbio.2025.106245","url":null,"abstract":"<div><h3>Objective</h3><div>This study assessed the chewing performance of dentate subjects, using mixing ability tests with two-colored chewing gums, and aimed to correlate the visual and optoelectronic measurement methods to provide a correspondence scale for predicting the standard deviation of hue (SD-Hue) values based on visual parameters and the number of chewing strokes.</div></div><div><h3>Design</h3><div>Two chewing gums were used (Hue-Check Gum® and Vivident Fruitswing®), and tests were performed with 1, 5, 10, 15, 20, 25, and 30 chewing cycles. The analysis included optoelectronic analysis to measure the level of color mixture (ViewGum software) expressed as the SD-Hue, ranging from 0 to 1, where lower values correspond to a higher level of mixture, and subjective analysis (SA) on a 5-point ordinal scale. Data analysis included bivariate correlation, definition of double-sided 90 % reference ranges, and GEE regression.</div></div><div><h3>Results</h3><div>There were significant correlations between SD-Hue and the number of chewing cycles and SA scores (p &lt; 0.001), and SA score was strongly correlated with the number of chewing cycles (p &lt; 0.001). A downward logarithmic curve for SD-Hue and SA can be observed according to the number of chewing cycles. The mixture level progressively increased by approximately 50 % for each five chewing cycles.</div></div><div><h3>Conclusion</h3><div>It was possible to correlate visual and optoelectronic methods and to establish a scale prediction of SD-Hue values based on SA and the number of chewing cycles. The color mixing measured by optoelectronic and visual methods was proportional to the number of chewing cycles. For every five cycles, the level of color mixture was reduced by 50 %, expressed in a downward logarithmic curve, independently from the chewing gum type.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106245"},"PeriodicalIF":2.2,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143747149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the effect of platelet-derived growth factor-BB on the biological activity of human mandibular bone marrow-derived mesenchymal stem cells","authors":"Yurika Oura,&nbsp;Masakazu Ishii,&nbsp;Haruka Miyata,&nbsp;Nao Ikeda,&nbsp;Tomoaki Sakurai,&nbsp;Fumio Suehiro,&nbsp;Naohiro Komabashiri,&nbsp;Masahiro Nishimura","doi":"10.1016/j.archoralbio.2025.106244","DOIUrl":"10.1016/j.archoralbio.2025.106244","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effects of platelet-derived growth factor-BB (PDGF-BB) on the biological activities of human mandibular bone marrow-derived mesenchymal stem cells (MBMSCs).</div></div><div><h3>Design</h3><div>PDGF-BB (20 ng/mL) was used to treat MBMSCs, and its effects on their proliferation, osteogenic differentiation, and migration were evaluated. Cell proliferation was evaluated using a WST-1 assay. Osteogenic differentiation was evaluated by measuring the mineralization potential and alkaline phosphatase activity. Cell migration was evaluated using wound healing and Transwell chamber assays. Cytoskeletal reorganization and adhesion dynamics were evaluated using immunofluorescence staining. Changes in intracellular signaling in MBMSCs induced by PDGF-BB stimulation were evaluated using western blotting. Furthermore, we investigated Girdin signaling as the molecular mechanisms underlying the regulation of PDGF-BB-induced cell migration.</div></div><div><h3>Results</h3><div>PDGF-BB treatment did not affect the proliferation or osteogenic differentiation of MBMSCs. PDGF-BB promoted the migration of MBMSCs. PDGF-BB treatment enhanced F-actin filament formation and paxillin localization at the leading edge of cells. PDGF-BB treatment activated Akt signaling in MBMSCs, and the inhibition of Akt signaling effectively suppressed PDGF-BB-induced Akt activation and migration. PDGF-BB promoted the phosphorylation of Girdin in MBMSCs, and the inhibition of Akt signaling attenuated PDGF-BB-induced Girdin activation.</div></div><div><h3>Conclusion</h3><div>This study demonstrated that PDGF-BB strongly induces the migration of MBMSCs without affecting their proliferation or osteogenic differentiation. Furthermore, PDGF-BB-induced migration of MBMSCs may be mediated through the Akt/Girdin signaling pathway. These findings provide important insight into the molecular mechanisms underlying PDGF-BB-induced periodontal tissue regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106244"},"PeriodicalIF":2.2,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143740029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macronutrient consumption in adults and association with oral and systemic parameters: A cross-sectional study","authors":"Larisse Santos Mendonça Alves ,&nbsp;Júlia Maria Munduri ,&nbsp;Israel Lacerda ,&nbsp;Letícia Gonçalves Reis ,&nbsp;Joana Rodrigues da Silva ,&nbsp;Cristine Miron Stefani ,&nbsp;Maria do Carmo Machado Guimarães ,&nbsp;Luana Severo Alves ,&nbsp;Solange Baraldi ,&nbsp;Naile Dame-Teixeira","doi":"10.1016/j.archoralbio.2025.106241","DOIUrl":"10.1016/j.archoralbio.2025.106241","url":null,"abstract":"<div><h3>Objective</h3><div>Oral conditions, such as tooth loss and changes in taste perception, can affect the consumption of dietary macronutrients. Our aim was to study systemic and oral alterations associated with inadequate consumption of macronutrients in adults and elderly with and without type 2 diabetes mellitus (T2D).</div></div><div><h3>Design</h3><div>This cross-sectional study included 170 individuals. Sociodemographic variables and clinical parameters were collected, such as weight status, waist circumference risk, T2D, smoking history, presence of removable dentures, worsening taste, among others. Outcomes included intakes of carbohydrates, lipids, fibers, and proteins. Poisson regression models were used to estimate prevalence ratios (PR) and 95 % confidence intervals (CI).</div></div><div><h3>Results</h3><div>Individuals reporting a worsening in taste perception were nearly four times more likely to have a high carbohydrate intake (PR=3.84; 95 % CI: 1.36–10.83). Saliva flow rate was significantly associated with increased lipid intake (PR=0.21; 95 % CI: 0.05–0.89). The presence of removable dentures in both arches (PR=1.40; 95 % CI: 1.07–1.81) and high cardiovascular risk (based on waist circumference) (PR=1.86; 95 % CI: 1.06–3.26) were associated with a higher prevalence of low fiber intake. These associations with fiber were influenced by saliva flow rate. Individuals aged ≥ 60 years (PR=1.12; 95 % CI: 1.00–1.24), using removable dentures in one arch (PR=1.23; 95 % CI: 1.04–1.46) or both arches (PR=1.22; 95 % CI: 1.04–1.45) had a higher prevalence of inadequate protein intake.</div></div><div><h3>Conclusions</h3><div>Demographic factors, systemic and oral conditions were significantly associated with inadequate macronutrient intake, highlighting the essential contribution of dentistry to addressing broader systemic health issues.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106241"},"PeriodicalIF":2.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Senescent vascular endothelial cells promote oral squamous cell carcinoma progression through complement C3 activation","authors":"Fangqi Jing ,&nbsp;Jingtian Mu ,&nbsp;Junjiang Liu ,&nbsp;Can Hu ,&nbsp;Fanglong Wu ,&nbsp;Qinghong Gao","doi":"10.1016/j.archoralbio.2025.106242","DOIUrl":"10.1016/j.archoralbio.2025.106242","url":null,"abstract":"<div><h3>Objective</h3><div>The tumour microenvironment (TME) plays a critical role in therapeutic response and clinical outcomes in cancer. Senescent stromal cells have been shown to promote tumour progression; however, the role of senescent vascular endothelial cells (VECs) in oral squamous cell carcinoma (OSCC) remains largely unknown. In this study, we aimed to explore the effects and potential mechanisms of senescent VECs in OSCC progression.</div></div><div><h3>Design</h3><div>Cisplatin was used to induce senescence in two endothelial cell lines. Senescence-associated β-galactosidase (SA-β-gal) staining, immunoblotting, cell cycle and proliferation assays, and migration and invasion assays were performed to access senescence development and biological behavior. Additionally, RNA sequencing analysis, multiplex immunohistochemical staining, immunoblotting, and xenograft mouse models were used to investigate the senescence-associated secretory phenotype of senescent VECs during OSCC progression and its potential molecular mechanisms.</div></div><div><h3>Results</h3><div>Cisplatin-induced senescent VECs exhibited senescence-related changes, including positive SA-β-gal expression and upregulation of p16, p21, and p53, along with attenuated proliferation and migration. Notably, cisplatin-induced VEC senescence promoted OSCC cell proliferation, migration, and invasion by activating complement C3. Increased gene and protein levels of C3 were observed in cisplatin-treated senescent VECs. Inhibition of C3 in vitro and in vivo reduced OSCC cell proliferation and invasion.</div></div><div><h3>Conclusion</h3><div>Senescent VECs induced by cisplatin promote OSCC proliferation and invasion through complement C3 activation. Targeting complement C3 in senescent VECs may offer a novel therapeutic strategy for OSCC treatment.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106242"},"PeriodicalIF":2.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143734760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akkermansia muciniphila enhances mucosal immunity against porphyromonas gingivalis","authors":"Qin Hu ,&nbsp;Wai Keung Leung ,&nbsp;Aneesha Acharya ,&nbsp;George Pelekos","doi":"10.1016/j.archoralbio.2025.106240","DOIUrl":"10.1016/j.archoralbio.2025.106240","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate the immunomodulatory potential of <em>Akkermansia muciniphila</em> in restoring <em>Porphyromonas gingivalis</em>-induced immune dysfunction.</div></div><div><h3>Design</h3><div>The immune response was assessed by measuring the expression of pro-inflammatory cytokines and antibacterial peptides in human gingival epithelial cells and human gingival fibroblasts treated with <em>A. muciniphila, P. gingivalis</em>, or a combination of both. Activation of the NF-κB pathway was analyzed using immunofluorescent staining and western blot. <em>In vivo</em> validation was performed using a mouse model, where <em>A. muciniphila</em> and <em>P. gingivalis</em> were administered alongside a MyD88-specific inhibitor to confirm the immunomodulatory mechanisms.</div></div><div><h3>Results</h3><div><em>A. muciniphila</em> significantly enhanced the defensive immune response through TLR-MYD88-NF-κB pathway. <em>In vitro</em> and <em>in vivo</em>, <em>A. muciniphila</em> upregulated chemokine expression to recruit immune cells. <em>A. muciniphila</em> also reduced the adhesion and internalization of <em>P. gingivalis</em> and increased the expression of genes encoding antimicrobial peptides (<em>DEFB103B</em> and <em>CAMP</em>).</div></div><div><h3>Conclusions</h3><div><em>A. muciniphila</em> demonstrates potential in combating <em>P. gingivalis</em> infection highlighting its role as a promising immune modulator for periodontal disease management.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106240"},"PeriodicalIF":2.2,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}