Archives of oral biology最新文献

{"title":"Dodecylmethylaminoethyl methacrylate inhibits the growth of Candida albicans and Enterococcus faecalis biofilm and the formation of osteoclast","authors":"Zong Yawen ,&nbsp;Shan Tiantian ,&nbsp;Shi Yangyang ,&nbsp;Li Yiling ,&nbsp;Lai Siyue ,&nbsp;Wei Yu ,&nbsp;Guo Xiao ,&nbsp;Li Bolei ,&nbsp;Ren Biao ,&nbsp;Cheng Lei","doi":"10.1016/j.archoralbio.2025.106331","DOIUrl":"10.1016/j.archoralbio.2025.106331","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the antimicrobial ability on the dual-species biofilm formed by <em>C. albicans</em> and <em>E. faecalis</em> and osteoclysis inhibition of DMAEM.</div></div><div><h3>Design</h3><div>DMAEM monomer was used to evaluate the antmicrobial ability on the dual-species biofilm of <em>C. albicans</em> and <em>E. faecalis</em> by biomass quantification, Scanning electron microscope and confocal laser scanning microscopy scanning, and RT-qPCR. The biosafty and inhibition ability on the dual-species biofilm and osteoclysis of the experimental root canal sealers containing DMAEM was tested by biomass quantification, Cell Counting kit-8, tartrate-resistant acid phosphatase staining, flow cytometry analysis, and RT-qPCR.</div></div><div><h3>Results</h3><div>The biomass of the dual-species biofilm formed by <em>C. albicans</em> and <em>E. faecalis</em> was significantly decreased by 26.3–51.3 % under 8–256 μg/mL DMAEM. 8 μg/mL DMAEM could reduce <em>E. faecalis’</em>s survival rate to 77.4 % (p &lt; 0.001) and down-regulated its virulence gene expression. Meanwhile, the mycelium count proportion of <em>C. albicans</em> in the dual<em>-</em>species biofilm was reduced from 50 % to 0 % (p &lt; 0.0001). The experimental sealers with DMAEM content could also decrease the survival rate of <em>C.albicans</em> and <em>E.faecalis</em> in the dual-species biofilm. Moreover, the elution of experimental root canal sealers containing ≤ 2.5 % DMAEM was biosafe and improved 81.0 % (p &lt; 0.05) osteoclastic inhibition compared with it of unmodified sealers at least.</div></div><div><h3>Conclusions</h3><div>The antimicrobial ability on the dual-species biofilm formed by <em>C. albicans</em> and <em>E. faecalis</em> and osteoclastic inhibition of DMAEM suggested its potential clinical application of DMAEM in the treatment of periapical inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106331"},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144313968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insight into perinatal health by investigating the neonatal line: A systematic review","authors":"Julia Estivals ,&nbsp;Elsa Garot ,&nbsp;Margaux Lamare ,&nbsp;David John Manton ,&nbsp;Adeline Le Cabec ,&nbsp;Christine Couture","doi":"10.1016/j.archoralbio.2025.106335","DOIUrl":"10.1016/j.archoralbio.2025.106335","url":null,"abstract":"<div><h3>Background</h3><div>The neonatal line (NNL) is an accentuated line visible in the enamel and dentine of primary teeth and in some of the cusps of the first permanent molars. It corresponds to the physiological stress associated with the event of birth. This study aimed to investigate the characteristics of the NNL, its relationship with prenatal and postnatal enamel, its correlation with health and life data of both contemporary and ancient individuals, and its potential to differentiate the prenatal from postnatal origin of enamel defects to better understand the life conditions of mother and child.</div></div><div><h3>Materials and methods</h3><div>Following PRISMA guidelines and using the PICO framework, a systematic review was conducted across MEDLINE, Scopus, DOSS, and Google Scholar covering literature from 1937 to 2023.</div></div><div><h3>Results</h3><div>A total of 1420 references were identified, from which 32 studies met the inclusion criteria. The selected studies examined the impact of maternal factors, perinatal conditions and gestation on the NNL. The width of the NNL varied according to maternal health and birth conditions. The NNL was also a marker of birth viability, with its presence indicating a live birth. Lastly, dietary shifts around birth were observed through variations in the perinatal enamel strontium/calcium ratio.</div></div><div><h3>Discussion and conclusion</h3><div>This study highlights the value of the NNL in reconstructing early life history by linking maternal health, prenatal and postnatal stress, and enamel development. Further research on the relationship between hypomineralized lesions in pathological enamel and the NNL could help identifying early (i.e., <em>in utero</em> or perinatal) risk factors.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106335"},"PeriodicalIF":2.2,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis promotes oral carcinogenesis through the NDK-ATP-P2X7 signal axis: An in vitro experimental study","authors":"Wei Wei ,&nbsp;Chen‑xi Li ,&nbsp;Mu-qiu Li ,&nbsp;Xiao-rong Tan ,&nbsp;Zhong‑cheng Gong","doi":"10.1016/j.archoralbio.2025.106332","DOIUrl":"10.1016/j.archoralbio.2025.106332","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate how <em>Porphyromonas gingivalis</em> (P. g) secreted nucleoside diphosphate kinase (NDK) affects the tumor-related biological behaviors of oral squamous cell carcinoma (OSCC) cells through NDK-ATP-P2X7 signal axis.</div></div><div><h3>Design</h3><div>An in vitro co-culture model that includes human OSCC cell lines (SCC 9 and SCC 25) together with P. g (W83 strain) and its NDK knockout variant (P. g-△NDK) were established to perform various key experiments to evaluate multiple malignant phenotypes, as well as analyze the relationship between adenosine triphosphate (ATP) and purinergic ligand-gated ion channel 7 (P2X7) receptor.</div></div><div><h3>Results</h3><div>In OSCC tissues, the levels of P. g and P2X7 were significantly higher than those found in adjacent normal tissues (<em>P</em> &lt; 0.0001). In cytological experiments, SCC9 and SCC25 cells infected with P. g exhibited enhanced proliferation, migration, and invasion capabilities (<em>P</em> &lt; 0.0001), increased cytotoxicity (<em>P</em> &lt; 0.0001), and decreased extracellular ATP content (<em>P</em> &lt; 0.001). After infection with <em>P</em>. g-△NDK, SCC9 and SCC25 cells showed enhanced proliferation, migration, and invasion abilities (<em>P</em> &lt; 0.0001), reduced cytotoxicity (<em>P</em> &lt; 0.0001), and increased extracellular ATP content (<em>P</em> &lt; 0.01). There was a positive correlation between P2X7 expression and extracellular ATP content; in contrast, adding a P2X7 inhibitor produced opposite results (all <em>P</em> &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>In SCC 9 and SCC 25 cells, P. g-△NDK reduces the hydrolysis of extracellular ATP by P. g, which enhances P2X7 expression and results in improved cellular proliferation, migration, and invasion capabilities, along with decreased cytotoxicity.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106332"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Associations between smoking, disease, and Porphyromonas gingivalis fimA genotype distribution: Insights from Korean periodontitis patients","authors":"Eun-Young Jang ,&nbsp;Seok Bin Yang ,&nbsp;Kyu Hwan Kwack ,&nbsp;Jae-Hyung Lee ,&nbsp;Ji-Hoi Moon","doi":"10.1016/j.archoralbio.2025.106333","DOIUrl":"10.1016/j.archoralbio.2025.106333","url":null,"abstract":"<div><h3>Objective:</h3><div>To investigate the distribution of <em>Porphyromonas gingivalis fimA</em> genotypes in relation to smoking and periodontitis severity in a Korean adult population, and to assess genotype-specific associations with host and environmental factors.</div></div><div><h3>Design:</h3><div>Subgingival biofilm samples were obtained from 283 adults, comprising 102 non-smoking healthy individuals, 98 non-smoking periodontitis patients, and 83 smoking periodontitis patients. Bacterial DNA was extracted and analyzed using <em>fimA</em>-specific PCR to detect <em>P. gingivalis</em> and its six <em>fimA</em> genotypes (I, II, III, IV, V, and Ib). Statistical analyses were performed using Fisher’s exact test and logistic regression models.</div></div><div><h3>Results:</h3><div>Type II <em>fimA</em> was the most common genotype across all groups and was significantly more prevalent in periodontitis patients. Fisher’s exact test revealed significant differences in genotype distribution between healthy and diseased individuals, except for types I and III. Logistic regression showed that <em>P. gingivalis</em> detection was strongly associated with disease stage (odds ratio: 7.5), with type II <em>fimA</em> linked to advanced disease. Types IV and Ib were significantly associated with smoking and age, suggesting differential responses of specific genotypes to environmental exposure.</div></div><div><h3>Conclusions:</h3><div>Given the distinct associations between <em>fimA</em> genotypes and smoking, careful consideration of strain selection is crucial for future studies investigating <em>P. gingivalis</em> pathogenesis. This study underscores the importance of genotype-specific microbial analyses in elucidating mechanisms of periodontitis progression and highlights the potential for environmental factors such as smoking to shape microbial variation at the strain level.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106333"},"PeriodicalIF":2.2,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144270312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular advances in adenoid cystic carcinoma: Genetic and epigenetic insights","authors":"Luciana Oliveira Almeida ,&nbsp;Kevin Luiz Lopes-Delphino ,&nbsp;Isabel Schausltz Pereira Faustino ,&nbsp;Lara Maria Alencar Ramos Innocentini ,&nbsp;Luiz Carlos Conti de Freitas ,&nbsp;Pedro Polastri Lima Peixoto ,&nbsp;Ana Carolina Fragoso Motta ,&nbsp;Lucas Dias de Oliveira ,&nbsp;Rogério Moraes Castilho ,&nbsp;Katiuchia Uzzun Sales","doi":"10.1016/j.archoralbio.2025.106323","DOIUrl":"10.1016/j.archoralbio.2025.106323","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to provide an overview of genetic and epigenetic alterations in adenoid cystic carcinoma, addressing challenges in prognosis, treatment, recurrence, and multidisciplinary management.</div></div><div><h3>Design</h3><div>A narrative review was conducted through searches in the PubMed, Scopus, Web of Science, EMBASE, LILACS and Google Scholar databases, using relevant keywords and including the verification of reference lists from journal articles through manual searching.</div></div><div><h3>Results</h3><div>The MYB-NFIB fusion emerged as a hallmark genetic feature of adenoid cystic carcinoma, influencing tumor behavior and resistance to apoptosis. Alterations in the PI3K/AKT and NOTCH pathways contributed to adenoid cystic carcinoma’s aggressive traits, such as perineural invasion and metastasis. Epigenetic modifications, including DNA methylation and histone changes, were also linked to tumor progression and poor prognosis. Proteases such as MMP-9 and cathepsins D and B are associated with aggressive disease and prognosis. Although adenoid cystic carcinoma's rarity challenges clinical trials, these molecular markers remain valuable therapeutic targets.</div></div><div><h3>Conclusion</h3><div>The molecular complexity of adenoid cystic carcinoma underscores the need for targeted therapies that address its specific genetic and epigenetic landscape. Future research should prioritize clinical trials focusing on molecularly targeted treatments and epigenetic interventions' role in improving adenoid cystic carcinoma outcomes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106323"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144255040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Permanent dental development radiographically assessed in children and adolescents with down syndrome - systematic review and meta-analysis","authors":"Maria Isabel Gomes França ,&nbsp;Debora Duarte Moreira ,&nbsp;Carolina Lima Lopes ,&nbsp;Fabiana Mantovani Gomes França ,&nbsp;Rizky Merdietio Boedi ,&nbsp;Ademir Franco","doi":"10.1016/j.archoralbio.2025.106322","DOIUrl":"10.1016/j.archoralbio.2025.106322","url":null,"abstract":"<div><h3>Objective</h3><div>To systematically revisit the existing scientific literature and investigate whether Down syndrome can affect the chronology of dental development in children and adolescents.</div></div><div><h3>Design</h3><div>A systematic literature review based on the Joanna Briggs Institute (JBI) Manual for Evidence Synthesis was developed and registered in The International Prospective Register of Systematic Reviews (PROSPERO, protocol: CRD #42024531685). The research protocol was designed according to the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols (PRISMA-P) and deposited in Zenodo repository (doi: 10.5281/zenodo.14993171). The strategic search was conducted in PubMed, LILACS, Scopus, SciELO, OATD and Open Grey. Risk of bias was assessed according to the JBI critical appraisal tool, while The Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool was used to assess the certainty of evidence. Subgroup meta-analyses were performed based on sex and dental age estimation method.</div></div><div><h3>Results</h3><div>Ten eligible studies were detected after 1864 initially screened. Standardized mean differences (SMD) between syndromic and non-syndromic individuals were not statistically significant (p &gt; 0.05). Differences between chronological and estimated ages were negligible (p &gt; 0.05) for both males and females. Nolla’s dental age estimation method was slightly more conservative and consistent than Demirjian’s (p &gt; 0.05). Heterogeneity (I²) was ≤ 3 %.</div></div><div><h3>Conclusion</h3><div>Down syndrome does not seem to significantly affect dental development of children and adolescents with and without Down syndrome when assessed radiographically. Caution is advised since the certainty of evidence of observational studies is naturally low.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106322"},"PeriodicalIF":2.2,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144254860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary proteome and microbiome in pregnancy and postpartum: An exploratory study on the relation with arterial hypertension","authors":"Maria João Azevedo ,&nbsp;Ana Filipa Ferreira ,&nbsp;Dmitry Loginov ,&nbsp;Petr Novák ,&nbsp;Inês Falcão-Pires ,&nbsp;Carla Ramalho ,&nbsp;Mark J. Buijs ,&nbsp;Bernd W. Brandt ,&nbsp;Egija Zaura ,&nbsp;Fábio Trindade ,&nbsp;Benedita Sampaio-Maia","doi":"10.1016/j.archoralbio.2025.106319","DOIUrl":"10.1016/j.archoralbio.2025.106319","url":null,"abstract":"<div><h3>Objectives</h3><div>Arterial hypertension (AH) influences salivary gland physiology and oral health, being associated with a higher incidence of periodontal disease in pregnant women. Evidence points to a bidirectional relationship between the oral microbiota and blood pressure regulation. Therefore, this study aimed to characterize the oral health of pregnant women and AH-associated changes in the salivary proteome and microbiome during pregnancy and postpartum.</div></div><div><h3>Design</h3><div>Ten healthy women and ten women with AH were enrolled. Saliva was collected during pregnancy and six months postpartum. The salivary proteome was characterized by shotgun label-free mass spectrometry analysis. Specific proteins were validated through parallel reaction monitoring (PRM). The oral microbiota was characterized via 16S rRNA gene amplicon sequencing (V4 region). The periodontal health and the caries history was assessed during pregnancy.</div></div><div><h3>Results</h3><div>Pregnant women with AH had lower junction plakoglobin (JUP)- and desmoplakin (DSP)-specific peptide levels than healthy women, confirmed by the PRM approach. The levels of these proteins correlated negatively with periodontal health indexes, which were higher in pregnant women with AH. In AH, nitrate-reducing microorganisms had lower abundance, correlating positively with JUP and DSP-specific peptides.</div></div><div><h3>Conclusions</h3><div>The salivary proteome and microbiota are shaped by AH during and after pregnancy. Further research is required to understand the underlying mechanisms impairing oral health in AH.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106319"},"PeriodicalIF":2.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is dental pulp inflammation capable of causing central inflammation, behavioral, and sensory alterations? A pre-clinical study","authors":"Iago Ramirez ,&nbsp;Igor Bassi Ferreira Petean ,&nbsp;Francisco Wanderley Garcia de Paula-Silva ,&nbsp;Aline Aparecida Ferraresi Tiballi ,&nbsp;Manoel Damião Sousa-Neto ,&nbsp;Fabiane Carneiro Lopes-Olhê ,&nbsp;Christie Ramos Andrade Leite-Panissi ,&nbsp;Jardel Francisco Mazzi-Chaves","doi":"10.1016/j.archoralbio.2025.106320","DOIUrl":"10.1016/j.archoralbio.2025.106320","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to assess local and central inflammatory aspects, behavioral changes, and alterations in mechanical and nociceptive peripheral sensitivities in a mouse model of experimentally induced pulpitis.</div></div><div><h3>Design</h3><div>Following Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines and ethics committee approval (231.1.395.59.0), 48 wild-type mice (C57BL/6) were allocated into two groups (n = 24): induced pulpitis and control. Pulpitis was induced under anesthesia by accessing the pulp tissue of the right lower first molar and inoculating 10 µL of lipopolysaccharide (LPS) suspension (1 µg/µL). Food intake, water intake, and sucrose at 1 % solution were daily evaluated. Both groups underwent evaluations for mechanical sensitivity (von Frey test) and nociceptive sensitivity (formalin test) at days 2, 5, and 10. Behavior evaluation (open field test) was performed at days 1, 2, 5, and 10. Post-euthanasia, oral tissues (bone and teeth) were collected for histological analysis, and the brain was preserved for Western blotting. Statistical analyses included two-way ANOVA and Fisher’s Exact Test (p &lt; 0.05).</div></div><div><h3>Results</h3><div>Mice with pulpitis showed significantly reduced food consumption on days 2 and 4. Mechanical allodynia was evident in the pulpitis group on days 1 and 2 compared to controls and baseline. Anxious-like behavior was more pronounced in the experimental group (p &lt; 0.05). No significant changes in nociceptive sensitivity were noted (p &gt; 0.05). Histological analysis revealed a higher count of inflammatory cells in the experimental group (p &lt; 0.05). Additionally, increased expression of Nuclear factor kappa B (NF-kB) and Glial fibrillary acidic protein (GFAP) in the amygdaloid complex was observed in the experimental group (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Experimentally induced pulpitis in mice led to mechanical allodynia and heightened anxious-like behavior, suggesting a potential link between local acute pulpitis and central inflammation via inflammatory marker expression in the amygdaloid complex.</div></div><div><h3>Clinical relevance</h3><div>There is consensus in the literature that acute pulpitis disrupts the patient's routine, affecting food and drink consumption, behavior, and causing suffering. However, the link between local inflammation and central inflammatory modulation remains unclear. This study provides insights into how pulp inflammation may affect local and central inflammation, as well as mechanical and peripheral nociceptive sensitivities, emotional changes, and food consumption. It establishes causal relationships, suggesting that central inflammatory stress could be a key factor, potentially guiding new therapeutic approaches based on an animal model.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106320"},"PeriodicalIF":2.2,"publicationDate":"2025-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144242485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of type 2 diabetes on ectopic calcification within the dentine-pulp complex","authors":"Howard Chao,&nbsp;Trudy Milne,&nbsp;Haizal Mohd Hussaini,&nbsp;Paul Cooper,&nbsp;Lara Friedlander","doi":"10.1016/j.archoralbio.2025.106316","DOIUrl":"10.1016/j.archoralbio.2025.106316","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the influence of Type 2 diabetes (T2D) on calcification within the dentine-pulp complex and examine the mRNA expression of mineralisation-associated genes in human dental pulp cells (hDPCs) cultured in a hyperglycaemic environment.</div></div><div><h3>Design</h3><div>Extracted non-carious molar teeth were collected from patients with well-controlled T2D (n<em>=</em>10) and non-T2D (controls) (n<em>=</em>10). The pulp was histologically examined using special stains. Primary hDPC lines (n = 3) were established from non-T2D tissue explants and grown in media containing 5.5mM- (control), 12.5mM- (prediabetes) and 25mM- D-glucose (T2D) for 7, 14 and 21 days. A PrestoBlue assay assessed the hDPC metabolic response to hyperglycaemia. The expression of mineralisation-associated genes <em>RUNX2, SPP1, SPARC, BGLAP, IBSP</em> and <em>DSPP</em> were analysed using quantitative real-time polymerase chain reaction. Data analyses were performed using GraphPad Prism and one-way ANOVA at <em>p</em> &lt; 0.05.</div></div><div><h3>Results</h3><div>Diffuse amorphous calcifications and irregular predentine were consistently observed in T2D samples. Culturing hDPCs in 12.5 mM and 25 mM glucose significantly increased their metabolic activity. All genes were detected in hDPCs in the presence of hyperglycaemia over time. However, with the exception of <em>RUNX2</em> which was initially downregulated in response to hyperglycaemia, all genes were expressed independent of glucose levels.</div></div><div><h3>Conclusion</h3><div>T2D is associated with pulp calcifications similar to other body sites. Diffuse fibro-dentine foci of calcifications resembled the appearance of an ‘aged’ pulp and the gene expression for markers of mineralisation was independent of glucose levels. Calcifications may form due to the effects of chronic inflammation and prolonged glucose exposure.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106316"},"PeriodicalIF":2.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral behaviors mediate the relationship between anxiety and painful temporomandibular disorders","authors":"Ambra Michelotti ,&nbsp;Rosaria Bucci ,&nbsp;Valeria Donnarumma ,&nbsp;Roberto Rongo ,&nbsp;Vittorio Simeon ,&nbsp;Iacopo Cioffi","doi":"10.1016/j.archoralbio.2025.106321","DOIUrl":"10.1016/j.archoralbio.2025.106321","url":null,"abstract":"<div><h3>Objectives</h3><div>Anxiety is strongly associated with chronic musculoskeletal pain, including painful temporomandibular disorders (p-TMD). Non-functional oral behaviors—such as wake-time tooth clenching or keeping the lower jaw in a tense position—are prevalent stress-related behaviors among individuals with elevated anxiety. These behaviors are thought to act as maladaptive coping strategies increasing strain on the masticatory muscles and the temporomandibular joint, thereby enhancing peripheral nociceptive input and contributing to the onset or persistence of p-TMD in individuals with high anxiety. While this behavioral pathway is theoretically supported, it has yet to be empirically verified. This study investigated whether non-functional oral behaviors mediate the relationship between anxiety and p-TMD.</div></div><div><h3>Design</h3><div>We recruited 299 adults with p-TMD (myofascial pain and/or arthralgia) and 374 pain-free controls. Anxiety levels and the frequency of non-functional oral behaviors were assessed using the Generalized Anxiety Disorder scale and the Oral Behavior Checklist, respectively. Mediation analysis was conducted to examine both the direct (anxiety → p-TMD) and indirect (anxiety → oral behaviors → p-TMD) pathways.</div></div><div><h3>Results</h3><div>Mediation analysis revealed that non-functional oral behaviors fully mediate the relationship between anxiety and p-TMD.</div></div><div><h3>Conclusion</h3><div>Non-functional oral behaviors are a key behavioral mechanism linking anxiety to p-TMD. These findings highlight the importance of targeting oral behaviors in interventions for TMD pain, particularly among individuals with high anxiety, and provide a foundation for future research into behavioral and neural mechanisms underlying TMD.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106321"},"PeriodicalIF":2.2,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144263178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}