Archives of oral biology最新文献

{"title":"Spatially resolved composition of bovine dental enamel in permanent incisors","authors":"Nathalie Murielly Rolim de Abreu ,&nbsp;Rudyard dos Santos Oliveira ,&nbsp;Frederico Barbosa de Sousa","doi":"10.1016/j.archoralbio.2025.106215","DOIUrl":"10.1016/j.archoralbio.2025.106215","url":null,"abstract":"<div><h3>Objective</h3><div>To quantify component volumes (mineral, organic, total water, firmly and loosely bound water volumes, and permeability) at histological points in bovine enamel and investigate their association with bovine enamel’s optical behavior and fracture toughness (FT).</div></div><div><h3>Design</h3><div>In vitro, cross-sectional, and experimental study. Longitudinal ground sections of ten permanent bovine incisors were analyzed using microradiography, polarizing microscopy, microhardness tester, and confocal laser fluorescence microscopy (CLFM). Continuous data were analyzed using regression and correlation tests.</div></div><div><h3>Results</h3><div>Bovine enamel exhibited gradients in both composition and FT from the surface inward, with the inner third showing positive birefringence in water immersion and opaque areas under immersion in air (explained by composition gradients), along with intermediate FT. Bovine enamel presented mineral volume of 79.6 % ( ± 2.7 %), water volume of 11.4 % ( ± 20.4 %), organic volume of 9.1 % ( ± 2.4 %), and permeability of 6.5 % ( ± 0.5 %). Water loss upon air drying was 1/5th of that in human enamel, representing a novel finding in enamel biology. The organic gradient was confirmed by CLFM. The ratio of firmly bound water to organic content was negatively associated with fracture toughness across the enamel layer.</div></div><div><h3>Conclusion</h3><div>bovine enamel differs from human enamel in terms of composition, birefringence, and permeability, requiring caution when interpreting results from studies using it to test dental products intended for humans. The composition of bovine enamel fully explained its optical behavior and partially explained its FT gradient.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106215"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaction risk increases significantly with each year of late third molar development in Western Canadian youths","authors":"Denver F. Marchiori ,&nbsp;Garnet V. Packota ,&nbsp;Prosanta Mondal ,&nbsp;Julia C. Boughner","doi":"10.1016/j.archoralbio.2025.106214","DOIUrl":"10.1016/j.archoralbio.2025.106214","url":null,"abstract":"<div><h3>Objective</h3><div>Third molar (M3) impaction affects millions of patients annually worldwide yet its etiology is unclear. Here we study an underexplored risk factor for impaction, later versus earlier M3 development, towards calculating impaction risk as a resource for clinicians.</div></div><div><h3>Design</h3><div>A total of 324 M3 areas (157 maxillary, 167 mandibular) were studied using retrospective CBCT scans and orthopantomograms of 99 Western Canadians (57 females, 42 males) aged 6–24 years. M3 crypt, crown and root development stage data were collected at an earlier timepoint T1 and then tested for association with M3 eruption status data documented at a later timepoint T2. This testing used Generalized Linear Mixed and Estimation modeling to explore relationships between M3 development stage and eruption status, and measure likelihood of versus functional emergence versus impaction risk (alpha level=0.05).</div></div><div><h3>Results</h3><div>Statistically, each year of late M3 development at T1 nearly doubled an M3’s risk of impaction at T2. Early-developing M3s had minimal impaction risk, as low as 5 %. This outcome was equivalent in upper and lower jaws and both sexes. Impacted M3s’ development was delayed due to later developmental onset, not to slower developmental rate.</div></div><div><h3>Conclusions</h3><div>Late-developing M3s are statistically more likely to become impacted. Clinical monitoring of M3 development from preadolescence is suggested. As early-developing M3s present minimal impaction risk, they are less likely to require prophylactic removal. This work provides foundational new insights into molar odontogenesis alongside practical information to help develop guidelines for managing patients with developing M3s.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106214"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orofacial pain symptoms in sleep bruxer or non-sleep bruxer: Insights from a population-based survey of puerperal women","authors":"Thiago Azario de Holanda ,&nbsp;Luana Patrícia Marmitt ,&nbsp;Juraci Almeida Cesar ,&nbsp;Noéli Boscato","doi":"10.1016/j.archoralbio.2025.106205","DOIUrl":"10.1016/j.archoralbio.2025.106205","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the prevalence of orofacial pain (OP) symptoms among a large sample of women who self-identified as sleep bruxers or non-sleep bruxers and who had given birth in 2019 in the municipality of Rio Grande, RS, Southern Brazil.</div></div><div><h3>Methods</h3><div>A standardized self-questionnaire was administered to assess OP symptoms. Chi-square tests were employed to compare proportions, and multivariate Poisson regression analysis with robust variance adjustment was used to estimate associations with sleep bruxers and non-sleep bruxers.</div></div><div><h3>Results</h3><div>A total of 2225 women were included in the study, with 78 (3.5 %) reporting OP symptoms. In the adjusted analysis, self-reported OP symptoms were significantly associated with non-sleep bruxer women living with three or more people in the household (PR=8.40; 95 % CI 1.85–38.11), as well as severe anxiety (PR= 4.73; 95 % CI 2.00–11.18). Among sleep bruxers, a significantly lower OP symptoms prevalence for those who were able to rely on their friends in times of distress (PR= 0.17; 95 % CI 0.10–0.30), while those with personality disorders exhibited significantly higher OP symptoms prevalence (PR= 3.30; 95 % CI 1.04–10.45).</div></div><div><h3>Conclusions</h3><div>The prevalence of OP symptoms was higher among non-sleep bruxer women living with three or more individuals in the household and among those experiencing severe anxiety. Indeed, sleep bruxer women with personality disorders had a higher prevalence of OP symptoms, while those with strong social support demonstrated a lower prevalence.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106205"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing analysis of Cutibacterium spp. recovered from health care-associated endodontic infections","authors":"Mónica Álvarez-Muñoz ,&nbsp;Christian Jerez-Olate ,&nbsp;Andrés Opazo-Capurro ,&nbsp;Raúl Alcántara-Dufeu ,&nbsp;Helia Bello-Toledo ,&nbsp;Gerardo González-Rocha ,&nbsp;Gabriela Sánchez-Sanhueza","doi":"10.1016/j.archoralbio.2025.106207","DOIUrl":"10.1016/j.archoralbio.2025.106207","url":null,"abstract":"<div><h3>Objective</h3><div><em>Cutibacterium acnes</em> (<em>C. acnes</em>) has been detected in culture and molecular studies of endodontic microbiota, indicating its potential as a neglected pathogen in endodontic infections. This study aimed to conduct a complete genomic analysis of three <em>Cutibacterium</em> spp. isolates obtained from persistent endodontic infections in patients from Chile.</div></div><div><h3>Design</h3><div>Whole genome sequencing and comprehensive genomic analysis using diverse bioinformatics platforms and databases were carried out.</div></div><div><h3>Results</h3><div><em>In-silico</em> analysis confirmed that strains A1 and E3 correspond to <em>C. acnes</em> species, while strain B1 corresponds to <em>C. namnetense</em>. Additionally, <em>C. acnes</em> strain A1 belongs to the phylotype IA₁, sequence type (ST) 4 and belongs to the clonal complex (CC) 4, whereas <em>C. acnes</em> strain E3 belongs to a novel ST. No antibiotic resistance, however, a variable distribution and identity of the VF was found.</div></div><div><h3>Conclusions</h3><div>Detailed analysis of genes associated with virulence factors shed light on the intrinsic pathogenic capacity of these strains, which may indicate differential adaptation to their specific niches or diversity in pathogenicity within the species.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106207"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STING regulates porphyromonas gingivalis lipopolysaccharide-induced pyroptosis and inflammatory response through the NF-κB/NLRP3 signaling pathway in human gingival fibroblasts","authors":"Bo Kou ,&nbsp;Yuna Zhang ,&nbsp;Wei Zhang,&nbsp;Jifang Zhang,&nbsp;Riwen Jiang","doi":"10.1016/j.archoralbio.2025.106197","DOIUrl":"10.1016/j.archoralbio.2025.106197","url":null,"abstract":"<div><h3>Objective</h3><div>The production of reactive oxygen species caused by antimicrobial response during periodontitis leads to the activation of NOD-like receptor protein 3 (NLRP3) inflammasome and pyroptosis. Stimulator of interferon genes (STING) has been found to be involved in regulating pyroptosis and inflammation in a variety of diseases. The present study aimed to investigate whether STING is involved in Porphyromonas gingivalis lipopolysaccharide (P.g LPS)-stimulated human gingival fibroblasts (HGFs) by regulating pyroptosis and inflammation.</div></div><div><h3>Design</h3><div>After culturing and identifying HGFs, HGFs were treated with P.g LPS. Constructs of si-STING were transfected into HGFs, which were then stimulated with P.g LPS for 24 h. Subsequently, cell viability, pyroptosis, inflammation, oxidative stress and alterations in the STING/TANK-binding kinase 1 (TBK1)/interferon regulatory factor 3 (IRF3)/nuclear factor-kappaB (NF-κB)/NLRP3 signalling pathway were detected.</div></div><div><h3>Results</h3><div>P.g LPS significantly enhanced STING expression in HGFs. Downregulation of STING rescued P.g LPS-enhanced pyroptosis, oxidative stress and inflammation in HGFs. Moreover, STING was found to bind directly to TBK1 to increase IRF3 phosphorylation and nuclear translocation of NF-κB, thus promoting NLRP3 inflammasome activation. Downregulation of STING rescued P.g LPS-enhanced TBK1/IRF3/NF-κB/NLRP3 pathway activation.</div></div><div><h3>Conclusion</h3><div>STING/TBK1/IRF3/NF-κB/NLRP3 is a key pathway governing pyroptosis, oxidative stress and inflammation of HGFs induced by LPS.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106197"},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic administration of polyphenols from dealcoholized red wine reduces inflammation and bone resorption in established apical periodontitis in male rats","authors":"Romulo de Oliveira Sales-Junior ,&nbsp;Bharbara de Moura Pereira ,&nbsp;Rafaela Ricci ,&nbsp;Nathália Evelyn da Silva Machado ,&nbsp;Julissa Denisse Arguello Alvarado ,&nbsp;Ana Beatriz Carreto ,&nbsp;Edilson Ervolino ,&nbsp;Anil Kishen ,&nbsp;Luciano Tavares Ângelo Cintra ,&nbsp;João Eduardo Gomes-Filho","doi":"10.1016/j.archoralbio.2025.106206","DOIUrl":"10.1016/j.archoralbio.2025.106206","url":null,"abstract":"<div><h3>Objective</h3><div>To analyze the effect of supplementation with dealcoholized red wine on the severity of apical periodontitis after it had been established.</div></div><div><h3>Design</h3><div>Thirty-two male Wistar rats were arranged into four groups: water as control, dealcoholized red wine, red wine, and alcohol. Apical periodontitis was induced by pulp exposure for 30 days when supplementation started and continued daily for 30 days. After the supplementation period, the rats were euthanized, and maxillae and jaws were removed to evaluate the inflammatory response and the bone resorption through microtomographic, histological and immunohistochemical analysis. Statistical tests were applied at 5 % significance.</div></div><div><h3>Results</h3><div>The dealcoholized red wine group demonstrated a significant reduction in bone resorption volume and increase in bone volume fraction and trabecular parameters (p &lt; 0.05). The dealcoholized red wine and red wine groups exhibited mild inflammation compared to the control and alcohol groups that showed moderate and severe inflammation respectively (p &lt; 0.05). The immunoreaction was significantly lower in the dealcoholized red wine group than in the control and alcohol groups for the TNF-α, IL-1β and TRAP cells (p &lt; 0.05). Furthermore, OPG in dealcoholized red wine was similar to red wine and control but higher than in the alcohol group (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Supplementation with dealcoholized red wine in rats decreased inflammation and bone loss in established apical periodontitis. This study highlights the potential of systemic administration of polyphenols from red wine in decreasing inflammation and bone resorption of established apical periodontitis in rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106206"},"PeriodicalIF":2.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the causal relationship between chronic pain and temporomandibular disorders: A two-sample Mendelian randomization study","authors":"Luyao Song ,&nbsp;Miaomiao Zhao ,&nbsp;Yingnan Wang","doi":"10.1016/j.archoralbio.2025.106191","DOIUrl":"10.1016/j.archoralbio.2025.106191","url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigates the causal effects of chronic pain on temporomandibular disorders using two-sample Mendelian randomization analysis.</div></div><div><h3>Design</h3><div>Two-sample bidirectional Mendelian randomization analysis was adopted to systematically explore the causal relationship between temporomandibular disorders and 7 types of chronic pain, including headache, hip, neck/shoulder, stomach/abdominal, back, knee, and facial chronic pain. Genetic variants from genome-wide association studies data served as instrumental variables. Inverse variance weighted, weighted median, and Mendelian randomization-Egger regression were applied, with sensitivity analyses to ensure robustness. And p &lt; 0.05 indicated statistical significance.</div></div><div><h3>Results</h3><div>Mendelian randomization analyses revealed a significant causal effect of neck or shoulder pain on the risk of temporomandibular disorders, with an Odds ratio of 6.317 (95 % CI: 1.730–23.062, p = 5.30E-3). Additionally, a reverse causal effect was observed where temporomandibular disorders may increase the risk of back pain, with an Odds ratio of 1.008 (95 % CI: 1.002–1.013, p = 8.40E-03). No significant causal relationships were found between other types of chronic pain (headache, hip pain, stomach or abdominal pain, facial pain, or knee pain) and temporomandibular disorders.</div></div><div><h3>Conclusions</h3><div>This study provides compelling evidence for the causal relationships between specific chronic pain conditions (neck or shoulder pain) and the risk of temporomandibular disorders. These insights highlight the importance of an integrated approach to patient care and emphasize the need for further research into the biological mechanisms underlying these relationships to guide the development of targeted preventive and treatment strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106191"},"PeriodicalIF":2.2,"publicationDate":"2025-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"BPIFA1 inhibits periodontitis by regulating the NF-κB/IκB signaling pathway and macrophage M1/M2 polarization","authors":"Hongyan Xu ,&nbsp;Tao Wang ,&nbsp;Ying Yang","doi":"10.1016/j.archoralbio.2025.106190","DOIUrl":"10.1016/j.archoralbio.2025.106190","url":null,"abstract":"<div><h3>Background</h3><div>Periodontitis is a chronic inflammatory disease characterized by tissue destruction and oxidative stress, primarily driven by the imbalance of immune responses. Bactericidal/permeability-increasing fold-containing family A member 1 (BPIFA1) has emerged as a key modulator of inflammation and immune homeostasis.</div></div><div><h3>Objectives</h3><div>This study investigates the role of BPIFA1 in periodontitis by focusing on its regulatory effects on the NF-κB/IκB signaling pathway and macrophage M1/M2 polarization.</div></div><div><h3>Methods</h3><div>Saliva and periodontal tissue samples were collected from 20 periodontitis patients and 20 healthy volunteers. BPIFA1 expression was analyzed using qRT-PCR and Western blot. In vivo studies were conducted in wild-type and BPIFA1-knockout (KO) mice, where periodontitis was induced via ligature placement and LPS injections. Oxidative stress markers (ROS, MDA, SOD), inflammatory cytokines (TNF-α, IL-6), and macrophage polarization markers (iNOS, CD86, Arg-1, CD206) were quantified. NF-κB pathway activation was assessed through Western blot analysis.</div></div><div><h3>Results</h3><div>BPIFA1 expression was significantly reduced in periodontitis patients and BPIFA1-KO mice. Loss of BPIFA1 resulted in increased oxidative stress, heightened NF-κB activation, and an imbalance in macrophage polarization, with increased M1 (pro-inflammatory) and decreased M2 (anti-inflammatory) macrophages. Additionally, BPIFA1 deficiency promoted Th17 differentiation and suppressed Treg cells, exacerbating periodontal inflammation.</div></div><div><h3>Conclusion</h3><div>BPIFA1 plays a critical role in inhibiting periodontitis progression by regulating the NF-κB/IκB signaling pathway and restoring macrophage M1/M2 balance. These findings highlight BPIFA1 as a potential therapeutic target for periodontitis management.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106190"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143437433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nanoencapsulation, biocompatibility and antibiofilm properties of chitosan/nisin Z spheres","authors":"Bhavya Rajan ,&nbsp;Dina Abdelmoneim ,&nbsp;Amira Samir Salem ,&nbsp;Georgios N. Belibasakis ,&nbsp;Rebecca Phemister ,&nbsp;Chen Chen ,&nbsp;Dawn Coates","doi":"10.1016/j.archoralbio.2025.106193","DOIUrl":"10.1016/j.archoralbio.2025.106193","url":null,"abstract":"<div><h3>Objectives</h3><div>To study antibiofilm formation properties and biocompatibility of nisin Z for oral applications by encapsulating it in a highly muco-adhesive chitosan nanosphere and testing its effects against a multispecies biofilm and <em>in vitro</em> wound healing assay.</div></div><div><h3>Materials and methods</h3><div>Nisin Z was encapsulated in chitosan. Encapsulation parameters were evaluated using dynamic light scattering and visualised with microscopy. The effects on biofilm growth were tested using a Calgary biofilm device against a five species biofilm model. The effects on human gingival fibroblast (HGF) growth and migration were examined using an Idibi (500 nm gap) migration assay and the percentage of gap closure measured using ImageJ.</div></div><div><h3>Results</h3><div>20,000 IU/ml (0.525 mg/ml production concentration) nisin Z in a 1:40 ratio of TPP:chitosan produced optimal nanospheres with a low polydispersity index of 0.498 and average size of 264.8 nm. The freeze-dried nanospheres have a dose-dependent effect on biofilm formation, significantly reducing biofilm attachment compared to the positive control. No colonies were observed for chitosan/nisin Z nanospheres at &gt; 6.25 mg/ml. HGFs in the control and 1000 IU nisin Z (unencapsulated) groups showed the most gap closure. The treatment group containing 50 mg/ml chitosan/nisin Z nanospheres showed gap closure comparable to the control.</div></div><div><h3>Conclusions</h3><div>Chitosan/nisin Z nanospheres were produced with antibiofilm activity with &gt; 6.25 mg/ml nanospheres, while up to 50 mg/ml had no significant effect on HGF gap closure <em>in vitro</em>. Thus, nanoencapsulated nisin Z has potential as a therapeutic agent for the treatment of periodontitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106193"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143429982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gold nanoparticle enhanced TNFα antibody interface using saliva for predicting prognosis in OSCC","authors":"K. Hema Shree ,&nbsp;R. Gayathri ,&nbsp;Vishnu Priya Veeraraghavan ,&nbsp;Pratibha Ramani ,&nbsp;Ramya Ramadoss ,&nbsp;Monal Yuwanati","doi":"10.1016/j.archoralbio.2025.106196","DOIUrl":"10.1016/j.archoralbio.2025.106196","url":null,"abstract":"<div><h3>Objectives</h3><div>To assess the efficacy of the AuNP-enhanced ELISA in the detection of salivary TNF-α in OSCC and evaluate its predictive value for survival.</div></div><div><h3>Design</h3><div>A longitudinal study was conducted on 40 OSCC patients and 10 healthy controls. Saliva was collected at a regular interval and TNF alpha levels were measured using ELISA and AuNP-enhanced ELISA. Descriptive statistics were carried out for demographic, clinical, and biomarker data. The efficacy of ELISA and AuNP-enhanced ELISA was estimated and compared using the ROC curve. Kaplan-Meier survival analysis and Cox proportional hazards models were used to assess the survival outcome. A neural network model was performed to estimate TNF-α levels over time in OSCC patients.</div></div><div><h3>Results</h3><div>OSCC patients (ELISA is 47.52 ± 20.23 and Gold nano-enhanced ELISA is 57.63 ± 24.99) exhibited significantly elevated TNFα levels compared to controls (ELISA is 10.13 ± 3.07and Gold nano-enhanced ELISA is 12.07 ± 3.66). Gold nano-enhanced ELISA (AUC of 0.995) demonstrated superior sensitivity than ELISA (AUC of 0.986), while Gold nano-enhanced ELISA achieves an even higher in detecting elevated TNFα levels. Kaplan-Meier analysis shows that Gold nano-enhanced ELISA outperforms ELISA in capturing survival trends, with better survival for TNF-α levels above the cutoff at 9 months (70 % vs. 60 %) and 24 months (40 % vs. 0 %). The neural network model poorly predicted TNF- levels over the period (AUC = 0.447).</div></div><div><h3>Conclusions</h3><div>The gold nano-enhanced TNFα detection method is effective in detecting TNFα levels between OSCC patients and controls, demonstrating superior sensitivity in identifying survival trends over time compared to traditional ELISA.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106196"},"PeriodicalIF":2.2,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}