Archives of oral biology最新文献

{"title":"PCSK9 is a passenger gene in head and neck cancer with minimal pathological influence","authors":"Hyun-Ji Kim ,&nbsp;Dong-Guk Park ,&nbsp;Su-Jung Choi ,&nbsp;Sung-Dae Cho","doi":"10.1016/j.archoralbio.2025.106302","DOIUrl":"10.1016/j.archoralbio.2025.106302","url":null,"abstract":"<div><h3>Objectives</h3><div>In this study, we aimed to explore the pathological effects of PCSK9 in head and neck cancer (HNC) by ablating its expression using the CRISPR-Cas9 knockout system.</div></div><div><h3>Design</h3><div>To investigate the clinical importance of PCSK9 in HNC, <em>in silico</em> analysis was performed using datasets from the GEO database and the UALCAN database. To evaluate the role of PCSK9 in HNC pathogenesis, both CRISPR-Cas9 knockout system and pharmacological inhibition were employed to ablate PCSK9 expression in HNC cell lines. The impact of PCSK9 on cellular growth and proliferation was assessed using Cell Counting Kit-8, soft agar, and clonogenic assays in a 2D culture model, as well as a hanging drop spheroid formation assay with live/dead staining in a 3D culture model. The involvement of PCSK9 in apoptosis induction was evaluated by detecting c-PARP expression through Western blotting, measuring the sub-G1 population via cell cycle assay, and verifying the Annexin V-positive population. Finally, changes in metastatic profiles associated with fluctuations in PCSK9 expression were examined using wound healing and transwell migration/invasion assays.</div></div><div><h3>Results</h3><div>In <em>in silico</em> analysis results, PCSK9 appeared to be related to the progression of HNC. However, experimental results demonstrated that PCSK9 plays a minimal role in cancer cell proliferation, anchorage-independent growth, colony formation capacity, in 2D cultures, as well as spheroidal growth in 3D cultures, and apoptosis induction. Furthermore, PCSK9 marginally influenced wound closure, metastatic potential, and invasive ability.</div></div><div><h3>Conclusion</h3><div>Collectively, these data suggest that PCSK9 serves a neutral role in HNC, functioning as a passenger gene.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106302"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abscisic acid ameliorates cognitive dysfunctions, but not facial allodynia, in a male rat nitroglycerin migraine model","authors":"Alireza Ahmadi ,&nbsp;Monavareh Soti ,&nbsp;Mohammad Reza Zarei ,&nbsp;Kristi Anne Kohlmeier ,&nbsp;Mohammad Shabani","doi":"10.1016/j.archoralbio.2025.106280","DOIUrl":"10.1016/j.archoralbio.2025.106280","url":null,"abstract":"<div><h3>Objective</h3><div>Abscisic acid (ABA), found in fruits and vegetables and produced in the human body, has anti-inflammatory, anti-oxidative, and pro-cognitive effects. The impact of ABA on trigeminal nerve hyperalgesia and cognitive behaviors in a rat migraine model was evaluated.</div></div><div><h3>Design</h3><div>Male rats (n = 8) were randomly distributed into 5 groups (Control, Sham, Abscisic acid (ABA), Nitroglycerin (NTG), and Nitroglycerin + Abscisic acid (NTG+ABA). Migraine was induced through 4 injections of NTG (5 mg/kg intraperitoneal (i.p.)). ABA (10 <em>µ</em>g/rat intracerebroventricular (i.c.v)) was administered 4 times with an interval of 24 hours. To evaluate trigeminal nerve hyperalgesia, the von Frey, acetone, and air puff tests were used. To evaluate locomotor and cognitive function of the animals, the open field, elevated plus maze (EPM), tail suspension (TST), Morris water maze (MWM), and passive avoidance (PA) tests were administered. Total antioxidant capacity levels (n = 5; TAC) were also monitored.</div></div><div><h3>Results</h3><div>Significant differences were observed between the surgically treated groups and the control group, but no differences in pain indexes were found in migraine animals when ABA was present. ABA-treated animals among the migraine groups showed reductions in anxiety-like and depression-like behaviors. Additionally, in the MWM test, the ABA animals exhibited improved spatial learning, while their memory did not show improvement. Avoidance learning and memory were not affected when evaluated using the PA. Increases in TAC were seen in ABA-treated animals in both serum and the spinal trigeminal nucleus.</div></div><div><h3>Conclusion</h3><div>Taken together, ABA reduces NTG-induced cognitive impairments, and antioxidant activity could play a role in the underlying ABA-mediated mechanism.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106280"},"PeriodicalIF":2.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaphase promoting complex subunit 10 is a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma","authors":"Lu Jia ,&nbsp;Haoyong Ning ,&nbsp;Ying Liu ,&nbsp;Pan Yang ,&nbsp;Xiaowei Cheng ,&nbsp;Bin Wang","doi":"10.1016/j.archoralbio.2025.106296","DOIUrl":"10.1016/j.archoralbio.2025.106296","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the role of anaphase promoting complex subunit 10 (ANAPC10) in both the diagnosis and prognosis of oral squamous cell carcinoma (OSCC).</div></div><div><h3>Design</h3><div><em>ANAPC10</em> expressions in OSCC tissues and adjacent normal tissues were analysed using TCGA and GEO databases. Its clinical prognostic significance was evaluated using the GEPIA tool. Signalling pathways associated with <em>ANAPC10</em> were identified through GO, KEGG, and GSEA. Promoter methylation levels of <em>ANAPC10</em> were assessed using the UALCAN tool. The correlation between <em>ANAPC10</em> expression and tumour-infiltrating immune cells was analysed using the TIMER database. <em>ANAPC10</em>’s role in OSCC cells was validated via CCK-8 assays, wound healing assays, cell migration assays, apoptosis assays, and cell cycle analysis.</div></div><div><h3>Results</h3><div><em>ANAPC10</em> expression was significantly elevated in OSCC tissues. Increased <em>ANAPC10</em> expression was associated with advanced T stages, pathological stages, histologic grades, and poorer therapeutic outcomes. Notably, high <em>ANAPC10</em> expression was strongly correlated with reduced overall survival, disease-specific survival, and progression-free interval in OSCC patients. Functional enrichment analyses revealed that <em>ANAPC10</em> is involved in RNA splicing, immune regulation, and cell cycle progression. Experimental validation further demonstrated that <em>ANAPC10</em> levels are influenced by promoter methylation status, and ANAPC10 regulates oral cancer cell proliferation, migration, apoptosis, and cell cycle progression.</div></div><div><h3>Conclusion</h3><div>ANAPC10 is a critical gene in OSCC prognosis, with roles in cell cycle regulation and RNA splicing. It may serve as a diagnostic biomarker and therapeutic target.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106296"},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc-alpha-2-glycoprotein overexpression and maintaining anti-apoptotic function in oral squamous cell carcinoma","authors":"Jureeporn Chuerduangphui ,&nbsp;Tipaya Ekalaksananan ,&nbsp;Chukkris Heawchaiyaphum ,&nbsp;Patravoot Vatanasapt ,&nbsp;Watchareporn Teeramatwanich ,&nbsp;Pensiri Phusingha ,&nbsp;Chamsai Pientong","doi":"10.1016/j.archoralbio.2025.106298","DOIUrl":"10.1016/j.archoralbio.2025.106298","url":null,"abstract":"<div><h3>Objective</h3><div>Overexpression of zinc-alpha-2-glycoprotein (ZAG) can be induced by various factors and has potential to be a biomarker in certain malignancies. However, in oral squamous cell carcinoma (OSCC), the risks and effects associated with ZAG overexpression are still poorly known. Here, we investigated the effect of HPV16 oncogenes and arecoline on the expression levels of ZAG and the possible effects of ZAG in OSCC cell lines.</div></div><div><h3>Design</h3><div>The level of ZAG expression was determined in protein extracted from exfoliated buccal cells from cancer-free control individuals and oral lesion cells from OSCC. Oral cell lines expressing HPV16E6/E7, and treated with arecoline were prepared to investigate ZAG expression. The effects of ZAG on cell biological activity and its targeting of UCP1 were determined in ZAG-overexpressing and ZAG-knockdown cells. <em>Results:</em> The expression of ZAG protein was significantly increased in oral lesion cells from OSCC relative to controls. Notably, the expression level of ZAG in OSCC positive for HPV, betel-quid chewing, and combination of both factors, was slightly higher than in cancer-free controls. ZAG expression was upregulated in oral cells treated with HPV16 oncoproteins E6 and/or E7, and treatment with arecoline (25 μg/ml). Interestingly, ZAG overexpression significantly increased <em>UCP1</em> and decreased apoptosis, whereas decreased <em>UCP1</em> and increased apoptosis were found in ZAG-knockdown cells. The mRNA expression levels of <em>TP53</em>, <em>STAT3</em>, <em>BCL2</em>, and <em>NFKB1</em> corresponded to observed anti-apoptosis function.</div></div><div><h3>Conclusions</h3><div>HPV oncoproteins and high doses of arecoline are risk factors for an overexpressed ZAG protein that has an anti-apoptotic function in OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106298"},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branched-chain amino acids promote gelatinase secretion from human periodontal ligament stem cells through nuclear factor kappa-B signaling","authors":"Xinjie Ning ,&nbsp;Huiling Zheng ,&nbsp;Ying Tu ,&nbsp;Qiang Guo ,&nbsp;Biao Ren ,&nbsp;Leng Wu ,&nbsp;Jing Xie ,&nbsp;Chengcheng Liu","doi":"10.1016/j.archoralbio.2025.106297","DOIUrl":"10.1016/j.archoralbio.2025.106297","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the effects of branched-chain amino acids (BCAAs) on periodontal tissues and regulation of gelatinase secretion by human periodontal ligament stem cells (hPDLSCs).</div></div><div><h3>Design</h3><div>The salivary BCAA levels (leucine, isoleucine, and valine) in the clinical participants were measured using mass spectrometry. A local injection model in the periodontium of Sprague Dawley rats was established to investigate the periodontal destruction induced by BCAAs. A BCAA-treatment model of hPDLSCs was established to detect the expression and activity of gelatinase and further explore the potential mechanism by which BCAAs enhance gelatinase secretion.</div></div><div><h3>Results</h3><div>Compared to the healthy controls, the salivary levels of leucine (<em>p</em> = 0.0190), isoleucine (<em>p</em> = 0.0351), and valine (<em>p</em> = 0.0072) were significantly elevated in individuals with periodontitis. <em>In vivo</em> experiments revealed that BCAAs aggravated periodontal extracellular matrix degradation and alveolar bone resorption in rats. Three-dimensional reconstruction of the rat maxilla demonstrated an increase in the distance from the cementoenamel junction to the alveolar bone crest (<em>p</em> &lt; 0.0001), and a decrease in the bone volume fraction (<em>p</em> &lt; 0.0001). <em>In vitro</em> experiments demonstrated that BCAAs activate the phosphorylation of nuclear factor kappa-B (NF-κB) signaling pathway in the hPDLSCs and consequently induce the secretion of gelatinases. The absence of any of the components in the BCAAs attenuated this effect.</div></div><div><h3>Conclusion</h3><div>BCAAs increase gelatinase secretion through the NF-κB (p-p65) signaling pathway, consequently exacerbating periodontal tissue destruction. This provides a novel insight on the role of BCAAs in the host immune-inflammatory response and increases our understanding of the possible involvement of BCAAs in the periodontitis development.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106297"},"PeriodicalIF":2.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Porphyromonas gingivalis lipopolysaccharide on the behavior of human dental pulp stem cells in vitro and their inflammation-related transcriptomics profile","authors":"Fei He ,&nbsp;Jingya Zhu ,&nbsp;Xiangni Zeng ,&nbsp;Li Jiang ,&nbsp;Lixia Zhang","doi":"10.1016/j.archoralbio.2025.106295","DOIUrl":"10.1016/j.archoralbio.2025.106295","url":null,"abstract":"<div><h3>Objective</h3><div>The inflammatory microenvironment in pulpitis and periapical periodontitis critically impairs dental pulp stem cells (DPSCs) functionality, yet the underlying molecular mechanisms remain poorly defined. This study aimed to investigate the effects of <em>Porphyromonas gingivalis</em> lipopolysaccharide (<em>P. gingivalis</em> LPS) on human DPSCs behavior and elucidate the transcriptomic changes underlying LPS-induced inflammation.</div></div><div><h3>Design</h3><div>Human DPSCs were exposed to 1 µg/mL LPS for 24 hours to establish an inflammatory model, with subsequent evaluation of proliferation (CCK-8), migration (Transwell), and odontogenic differentiation (ALP staining, mineralization assays and odontoblast markers expression). Cell cycle progression was quantified through flow cytometry, while RNA sequencing analysis delineated transcriptional alterations.</div></div><div><h3>Results</h3><div>LPS exposure significantly attenuated DPSCs proliferative capacity (<em>P</em> &lt; 0.01), suppressed migration (<em>P</em> &lt; 0.01), and impaired odontogenic differentiation, evidenced by diminished ALP activity, mineralized nodule formation and odontoblast markers expression. Cell cycle analysis revealed G0/G1 phase arrest (<em>P</em> &lt; 0.01), indicating proliferative quiescence. Transcriptomic profiling identified 467 differentially expressed genes. <em>P. gingivalis</em> LPS exerts its effects on DPSCs by concurrent activation of both canonical (Toll-like receptors, TLRs) and non-canonical (Nucleotide-binding oligomerization domain-like receptor) signaling pathway. Pathway enrichment revealed the enrichment of the several important signaling pathways such as Phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt), tumor necrosis factor (TNF) /Nuclear factor kappa B (NF-κB) and interleukin (IL) −17 signaling under LPS stimulation.</div></div><div><h3>Conclusion</h3><div><em>P. gingivalis</em> LPS disrupts DPSCs regenerative functions by modulating inflammatory and developmental signaling pathways, providing mechanistic insights into impaired pulp repair during oral infections.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106295"},"PeriodicalIF":2.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of experimental polymer-containing solutions in preventing caries-like lesions on enamel","authors":"Raissa Manoel Garcia ,&nbsp;Astrid Carolina Valdivia Tapia ,&nbsp;Alessandra Buehler Borges ,&nbsp;George J. Eckert ,&nbsp;Frank Lippert ,&nbsp;Tais Scaramucci Forlin ,&nbsp;Anderson T. Hara","doi":"10.1016/j.archoralbio.2025.106286","DOIUrl":"10.1016/j.archoralbio.2025.106286","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate experimental solutions containing polymers (Chitosan: CHI, Sodium linear polyphosphate: LPP, Polyacrylic acid: PAA; and 2-methacryloyloxyethyl phosphorylcholine: MPC) isolated, associated or not with fluoride (F), stannous ions (Sn), or F+Sn <em>in vitro</em> against caries-like lesion in enamel.</div></div><div><h3>Design</h3><div>Bovine enamel sections were randomly allocated into 20 groups (n = 14): Distilled water (DIW, negative control), F: 220 ppm F^-, Sn: 800 ppm Sn²⁺, F+Sn, and four polymers (0.5 % CHI, 2 % LPP, 0.1 % PAA, 2 % MPC) associated or not to F, Sn, or F+Sn. The single sections were individually subjected to a pH cycling: demineralization (3 h, 2 ×/d), experimental solution (1 min, 2 ×/d), and remineralization (overnight), for 5d. The enamel sections were analyzed using digital transverse microradiography (integrated mineral loss-ΔZ, and lesion depth-L). Data were analyzed using Kruskal-Wallis and Wilcoxon rank sum tests, with alpha= 0.05.</div></div><div><h3>Results</h3><div>When tested in isolation, LPP was the only polymer affording greater protection than DIW, while PAA enhanced ΔZ [median (Q1-Q3); vol%min× µm: 2530 (2070–3140) vs. 3710 (3410–3860) vs. 4375 (3820–4990) respectively; all p &lt; 0.05]. None of the polymers could increase the protection provided by F [1430 (1340,1570), p = 0.084] or F+Sn [1515(1150–1940), p = 0.182)]. CHI [2525(1890–3210), p = 0.01] and LPP [3105 (2460–3250), p = 0.006] increase the protection offered by Sn [3500 (3350–3790)], but PAA enhanced ΔZ [4340 (3540–4820), p = 0.044]. For L data, there are no polymer effects compared to DIW [93.30(82.60–104.80), p = 0.177], F [78.25(70.40–90.30), p = 0.340] or F+Sn [73.05(54.60–81.00), p = 0.056]. Only MPC decrease the degree effect of Sn [113.10 (110.40–124.30) and 99.20(92.60, 105.80), p = 0.003].</div></div><div><h3>Conclusions</h3><div>LPP was the only polymer that protected enamel against demineralization when tested in isolation. However, none of the polymers could increase the protection offered by fluoride or fluoride plus stannous ions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106286"},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distributions of orofacial two-point discrimination threshold: A cross-sectional study in healthy population and patients with pain-related temporomandibular disorders","authors":"Yifei Long,&nbsp;Yihang Fu,&nbsp;Tianyi Chen,&nbsp;Yunhao Zheng,&nbsp;Li Chen,&nbsp;Tiqian Liu,&nbsp;Jun Wang,&nbsp;Xin Xiong","doi":"10.1016/j.archoralbio.2025.106284","DOIUrl":"10.1016/j.archoralbio.2025.106284","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to explore the distribution and difference of orofacial two-point discrimination threshold (2-PDT) between patients with pain-related Temporomandibular Disorders (TMDs) and healthy individuals.</div></div><div><h3>Methods</h3><div>This study included 58 pain-related TMDs patients and 58 healthy individuals. Demographics including the gender, age of participants were collected. Temporomandibular joint (TMJ), mandible, zygomatic and temporal 2-PDTs were examined for statistical analyses.</div></div><div><h3>Results</h3><div>Pain-related TMDs patients showed significantly higher 2-PDTs in all tested regions (<em>p</em> &lt; 0.001). In gender subgroups of the control, males had significantly higher 2-PDTs than females in bilateral TMJ and temporal regions. In gender subgroups of patients, no statistical 2-PDT differences were found between males and females. Pain-related TMDs females had higher 2-PDTs than control females in all regions, which were also observed between pain-related TMDs males and control males (<em>p</em> &lt; 0.001), except for temporal 2-PDTs. Only pain-related TMDs were significantly associated with orofacial 2-PDTs with β coefficients varied from 3.5 to 7 (<em>p</em> &lt; 0.001). The discrimination performance of 2-PDTs on pain-related TMDs was considered effective, while the TMJ 2-PDTs was the best single predictor (Area under curve: 0.833).</div></div><div><h3>Conclusion</h3><div>Pain-related TMDs patients had weaker orofacial tactile acuity, which was consistent in gender subgroups, except for the temporal region of males. Healthy males had weaker tactile acuity compared to females in TMJ and temporal regions. No significant gender effect on orofacial tactile acuity was observed among male and female patients. The 2-PDTs of the tested sites, especially for TMJ, has the potential for further utilization in pain-related TMDs patients.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106284"},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of dose and time effects of flavonoids, alone or in combination, on the induction of mineralization markers in human osteoblast-like cells","authors":"Rafaela Laruzo Rabelo ,&nbsp;Gabriel Pereira Nunes ,&nbsp;Geórgia Rondó Peres ,&nbsp;Rafael Araújo Rios ,&nbsp;Maria Eduarda de Souza ,&nbsp;Gabriela Pacheco de Almeida Braga ,&nbsp;Cristiane Duque","doi":"10.1016/j.archoralbio.2025.106285","DOIUrl":"10.1016/j.archoralbio.2025.106285","url":null,"abstract":"<div><h3>Objective</h3><div>This study compared the cytotoxicity, alkaline phosphatase (ALP) activity, and mineralization-inducing effects of flavonoids on human osteoblast-like Saos-2 cells.</div></div><div><h3>Design</h3><div>Saos-2 cells were treated with quercetin, myricetin, pinocembrin, kaempferol, isoquercitrin (Iso), rutin (Rut), taxifolin (Tax), ampelopsin (Amp), epigallocatechin-3-gallate (EGCG), and chrysin at 100, 50, and 25 µM for 48 h. Metabolic activity, ALP activity and mineral deposition were assessed via resazurin, thymolphthalein and Alizarin Red S staining assays, respectively, after 8 and 14 days. Calcium hydroxide was used as positive control, and untreated cells (DMEM) as negative control. Based on initial screening, Tax, Iso, Rut and Amp were selected for double combination treatments (at 50/50 µM and at 25/25 µM), and the same assays were performed. Data were analyzed using ANOVA and Tukey's test (α = 0.05).</div></div><div><h3>Results</h3><div>Comparing the flavonoids, regardless of time and concentration, only chrysin at 100 µM and 50 µM significantly reduced cell viability. Iso, Rut, Tax, and Amp exhibited the highest ALP activity and mineralized nodules formation, significantly outperforming the other flavonoids and DMEM control, particularly at 50 and 25 µM (p &lt; 0.05). Among the combinations, Tax+Iso, Tax+Amp and Tax+Rut, at 25/25 µM demonstrated higher ALP activity and enhanced mineral deposition compared to the other flavonoid combinations and DMEM (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Based on this preliminary in vitro model, Tax, Iso, Rut, and Amp, both individually and in combination, effectively promote biomineralization in Saos-2 cells, without inducing cytotoxic effects. These flavonoids hold significant potential for osteogenic applications, warranting further in vivo studies and clinical trials to optimize their therapeutic use.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106285"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of omega-3 and omega-6 fatty acids on tumor suppressor pathways in mice tongue oral epithelial dysplasia","authors":"Julieta Don ,&nbsp;Victoria Ferrero ,&nbsp;Tamara Mazo ,&nbsp;Sol Bernárdez ,&nbsp;Nelso Barotto ,&nbsp;Mabel Brunotto ,&nbsp;María Eugenia Pasqualini","doi":"10.1016/j.archoralbio.2025.106283","DOIUrl":"10.1016/j.archoralbio.2025.106283","url":null,"abstract":"<div><h3>Objective</h3><div>This work aimed to evaluate the modulating effect of polyunsaturated fatty acids omega-3 and omega-6 on the expression of TP53 and specific primiRNAs 34 b/c in the development of premalignant characteristics in tongue epithelial dysplasias in-vivo experimental models</div></div><div><h3>Design</h3><div>BALB/c mice were divided into two diet groups: fish oil rich in omega-3 and corn oil rich in omega-6. Tongue dysplasia was generated by applying a 0.5 % solution of 7,12-dimethylbenzantracene (DMBA) in acetone. After sacrificing, the animals were assessed for omega-6 and omega-3, TP53/Ki67 immunostaining, Trp53R270H mutations, and primiRNA 34 b/c expression.</div></div><div><h3>Results</h3><div>Mice fed omega-6 showed higher dysplasias and Ki67 immunostaining percentages than those fed omega-3-rich oils. Furthermore, mice fed with corn oil rich in omega-6 showed significantly high average arachidonic-acid values, while the group fed cod oil rich in omega-3 showed substantially higher average values of Ecosapentanoic acid and Docosahexanoic acid in tongue tissue cell membranes. The primiRNA-34 b/c was upregulated in animals fed corn oil, enriched-omega-6 at 90 days.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that dietary lipids influence the development of DMBA-induced premalignant features on the tongue of mice; therefore, a low intake of omega-6-rich oils and a higher intake of omega-3 could be recommended for preventing features similar to mucosal dysplasia, which could be an important preventive strategy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106283"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}