Archives of oral biology最新文献

{"title":"Bioactive caries-preventing effects of mineral ions released from surface pre-reacted glass-ionomer (S-PRG) filler on oral biofilm","authors":"Kazuo Kato ,&nbsp;Ryo Kutsuna ,&nbsp;Yoshiaki Kawamura ,&nbsp;Yoshihiro Shimazaki","doi":"10.1016/j.archoralbio.2025.106392","DOIUrl":"10.1016/j.archoralbio.2025.106392","url":null,"abstract":"<div><h3>Objective</h3><div>The bioactive caries-preventing effects of mineral ions released from surface pre-reacted glass-ionomer (S-PRG) filler on oral biofilm were estimated by analyzing the depth-specific density of <em>Streptococcus mutans</em> and <em>Streptococcus sanguinis</em>.</div></div><div><h3>Design</h3><div>Ten participants wore <em>in situ</em> plaque-generating enamel slab devices on their upper molars to form biofilm for 5 days. Filtrates were prepared from prophylaxis paste slurries containing S-PRG filler, whereas paste without the filler served as the control. The devices were alternately immersed in sucrose solution three times and in the experimental or control filtrate twice each day. Two biofilm samples taken from each device were separated into 6–9 layered fractions (100 μm each) by serial sectioning. One sample was used for extraction of genomic DNA. The gene sequences encoding D-alanine:D-alanine ligases were amplified using universal and species-specific primers. Borate, aluminum, silicate, and strontium concentrations were determined using thicker sections of the other sample, correcting for biomass volume estimated by the area measurement of stained thinner sections.</div></div><div><h3>Results</h3><div>The ratios of <em>S. mutans</em> to <em>S. sanguinis</em> in the outer, middle, and inner layers were 0.0359, 0.0254 and 0.0157 for the experimental biofilm and 0.046, 0.0325 and 0.0255 for control biofilm, respectively. The ratio in the whole layer was significantly lower in the experimental biofilm than control biofilm. Strontium and aluminum concentrations in the experimental biofilm were 114- to 146-fold and 2.74- to 4.48-fold higher compared with control biofilm. respectively.</div></div><div><h3>Conclusions</h3><div>These results suggest that S-PRG filler has bioactive caries-preventing effects on oral biofilm.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106392"},"PeriodicalIF":2.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective bacterial proteolysis in periodontal disease: Molecular mechanisms and therapeutic implications, a narrative review","authors":"Andrea Escalante-Herrera,&nbsp;Walter L. Siqueira","doi":"10.1016/j.archoralbio.2025.106391","DOIUrl":"10.1016/j.archoralbio.2025.106391","url":null,"abstract":"<div><h3>Background/Objective</h3><div>Periodontal diseases are characterized by complex interactions between the immune system, oral bacteria, host tissues, and various external and internal factors. A hallmark of disease progression is the selective degradation of host proteins by bacterial proteases. This review explores the molecular mechanisms of bacterial proteolysis, with a focus on the selectivity toward host proteins essential for oral homeostasis.</div></div><div><h3>Methods</h3><div>An extensive electronic search was conducted using PubMed, Scopus, and Web of Science, covering the last 20 years. Search terms included “degradation,” “oral tissue,” “proteins,” “bacteria,” “periodontal disease,” “periodontitis,” “pathways,” and “host.” Boolean operators and filters were applied to refine results. Articles were evaluated based on titles, abstracts, and full texts. Additional references were identified through citation tracking. A narrative synthesis approach was used to integrate findings.</div></div><div><h3>Results</h3><div>Bacterial proteases exhibit precise substrate recognition, targeting specific structural, immune, and signaling proteins in the oral environment. The proteolytic profile and disease pathology are shaped by microbial community dynamics, including cooperative and competitive interactions. Host factors such as genetic diversity, local microenvironmental conditions, and immune responses further modulate protein degradation. Despite advancements, critical gaps remain, particularly regarding post-translational modifications and the determinants of proteolytic specificity.</div></div><div><h3>Conclusions</h3><div>Selective bacterial proteolysis plays a pivotal role in periodontal disease pathogenesis. Addressing current knowledge gaps through advanced proteomics, longitudinal studies, and imaging technologies is essential for developing targeted diagnostic and therapeutic strategies. An integrative approach is needed to enhance periodontal disease management and improve patient outcomes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106391"},"PeriodicalIF":2.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic supplementation attenuates dental pain and inhibits pain-induced cognitive impairment in male rats","authors":"Pegah Abazari-Bozhgani ,&nbsp;Saeed Esmaeili-Mahani ,&nbsp;Mehdi Abbasnejad ,&nbsp;Maryam Raoof ,&nbsp;Frank Lobbezoo","doi":"10.1016/j.archoralbio.2025.106382","DOIUrl":"10.1016/j.archoralbio.2025.106382","url":null,"abstract":"<div><h3>Objectives</h3><div>The gut-brain axis has emerged as a promising avenue for understanding the bidirectional communication between the gut microbiota and the central nervous system. This study investigated the potential impact of probiotics, <em>Lactobacillus acidophilus</em> (LA-5), <em>Lactobacillus paracasei</em> (<em>L. casei</em> 431), and <em>Bifidobacterium lactis</em> (BB-12), as well as their combination, on dental pulp pain management and cognitive functions.</div></div><div><h3>Design</h3><div>Forty-eight male Wistar rats were randomly assigned to six experimental groups (n = 8). The probiotics (10⁹ CFU) were orally administered for 14 consecutive days. Capsaicin (100 µg) was used to induce inflammatory pulp nociception. The Morris water maze task was used to evaluate learning and memory performance. Levels of the interleukin-1 beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) cytokines in the animals' trigeminal ganglion (TG) and the brain-derived neurotrophic factor (BDNF), neuropeptide Y (NPY), tropomyosin receptor kinase B (Trk-B) and Cyclooxygenase-2 (COX-2) genes in the animals' hippocampus were determined using western blotting and RT-PCR, respectively.</div></div><div><h3>Results</h3><div>Intradental application of capsaicin induced nociceptive behavior and increased IL-1β and TNF-α in the TG of rats. Probiotics could attenuate nociception and prevent IL-1β and TNF-α overexpression. Furthermore, pain induced cognitive impairments, decreased BDNF, NPY, and Trk-B and increased COX-2 gene expression in rat hippocampus, which were inhibited by probiotics supplementation.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that probiotics may play a role in orofacial pain relief and cognitive enhancement in painful situations by modulating gut microbiota composition and influencing protein levels and gene expression in brain regions associated with pain and cognition.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106382"},"PeriodicalIF":2.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of parecoxib and dexamethasone on the temporomandibular joint of orchiectomized rats: Morphological and immunological analysis","authors":"Victor Augusto Benedicto dos Santos ,&nbsp;Francisco Carlos Groppo ,&nbsp;José Roberto Garcia de Toledo ,&nbsp;Michael Henrique Araújo Monteiro ,&nbsp;Guilherme Elias Pessanha Henriques ,&nbsp;Sidney Raimundo Figueroba","doi":"10.1016/j.archoralbio.2025.106381","DOIUrl":"10.1016/j.archoralbio.2025.106381","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of parecoxib (a selective COX-2 inhibitor) and dexamethasone (a corticosteroid) on the temporomandibular joint (TMJ) of orchiectomized rats, a model of testosterone deficiency, through histological and immunological analyses.</div></div><div><h3>Methods</h3><div>Thirty-six rats were divided into six groups (n = 6). Sham groups received saline, parecoxib (0.3 mg/kg), or dexamethasone (0.1 mg/kg). ORX groups received the same treatments. TMJs were processed for histological staining (toluidine blue and picrosirius red) and analyzed by histomorphometry, measuring total cartilage thickness and its layers (fibrous, proliferative, mature, hypertrophic). Cytokines (IL-1α, IL-1β, IL-6, TNF-α) were quantified by ELISA. Data were analyzed using ANOVA-Welch and Kruskal-Wallis tests.</div></div><div><h3>Results</h3><div>ORX increased IL-1α, IL-1β, and TNF-α levels. IL-6 was reduced by dexamethasone. Dexamethasone also decreased cartilage thickness and accelerated its differentiation into subchondral bone. In contrast, parecoxib preserved cartilage thickness, especially in the fibrous and proliferative layers, and increased proteoglycan content. Both drugs reduced inflammatory markers, but with distinct structural effects.</div></div><div><h3>Conclusions</h3><div>Testosterone deficiency enhanced TMJ inflammation and impaired cartilage structure. While both dexamethasone and parecoxib modulated these effects, their actions differed: dexamethasone promoted cartilage-to-bone differentiation, potentially unfavorable long term, whereas parecoxib preserved cartilage integrity. These findings underscore hormonal influence and support selective anti-inflammatory strategies for TMJ preservation under androgen deficiency.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106381"},"PeriodicalIF":2.1,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and characterization of the extracellular matrix in salivary gland tumors through histochemistry and second harmonic generation","authors":"Maria Clara Falcão Ribeiro-de-Assis ,&nbsp;Luccas Lavareze ,&nbsp;Antonio Thiago Pereira Campos ,&nbsp;Sílvio Roberto Consonni ,&nbsp;Arthur Antolini-Tavares ,&nbsp;Érika Said Abu Egal ,&nbsp;Albina Altemani ,&nbsp;Fernanda Viviane Mariano","doi":"10.1016/j.archoralbio.2025.106380","DOIUrl":"10.1016/j.archoralbio.2025.106380","url":null,"abstract":"<div><h3>Objective</h3><div>To describe extracellular matrix (ECM) components in salivary gland tumors with and without myoepithelial differentiation.</div></div><div><h3>Design</h3><div>Five cases of each tumor type were analyzed: pleomorphic adenoma, myoepithelioma, adenoid cystic carcinoma, myoepithelial carcinoma, epithelial-myoepithelial carcinoma, and salivary duct carcinoma. Histochemical stains identified collagen fibers, elastin, proteoglycans (PG), and glycosaminoglycans (GAG). Collagen was further examined by second harmonic generation (SHG) in three regions: tumor stroma, extratumoral stroma, and capsule. ImageJ was used to analyze area fraction, mean gray level, entropy, and contrast. Data underwent statistical analysis (p &lt; 0.0001).</div></div><div><h3>Results</h3><div>ECM composition varied among salivary gland tumors. Pleomorphic adenoma showed low elastin and collagen, but PG and GAG were present in over 50 % of cases. Myoepithelioma showed variable collagen and elastin, while adenoid cystic carcinoma had predominant collagen. Myoepithelial carcinoma presented inconsistent elastin and low collagen. Epithelial-myoepithelial carcinoma and salivary duct carcinoma showed intense collagen staining. SHG revealed higher area fraction and gray level in the extratumoral region. Entropy was significantly higher outside the tumor. Malignant tumors with myoepithelial differentiation showed increased values in all SHG parameters compared to benign counterparts.</div></div><div><h3>Conclusions</h3><div>Benign tumors expressed more PG and GAG, whereas malignant ones had increased collagen with structural alterations. SHG analysis indicated lower collagen intensity within tumors and highlighted the role of ECM remodeling in malignancy, invasion, and metastasis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106380"},"PeriodicalIF":2.1,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic promotion of biomimetic enamel remineralization by amelogenin, amelotin, and ameloblastin C-terminal peptide in vitro","authors":"Xiu Zhong ,&nbsp;Lu Yin ,&nbsp;Peng Zhang ,&nbsp;Shaozhen Ma ,&nbsp;Xiaohua Ren ,&nbsp;Kun Tian","doi":"10.1016/j.archoralbio.2025.106379","DOIUrl":"10.1016/j.archoralbio.2025.106379","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the influence of human amelogenin (AM), amelotin (AMTN), and ameloblastin c-terminal peptide (AMBN-C), both individually and in combination, on the in vitro remineralization of enamel.</div></div><div><h3>Design</h3><div>AM, AMTN, and AMBN-C were recombined and purified in vitro and formulated into 200 µg/ml chitosan (CS) protein gels. Nine different gel groups, including saline, CS, CS-AM, CS-AMTN, CS-AMBN-C, CS-AM-AMTN, CS-AM-AMBN-C, CS-AMTN-AMBN-C, and CS-AM-AMTN-AMBN-C, were applied to artificial carious tooth samples. These samples were incubated in artificial saliva for seven days, and the enamel surface's mineralization was assessed using SEM and XRD.</div></div><div><h3>Results</h3><div>CS-AM, CS-AMTN, and CS-AMBN-C stimulated the formation of scattered \"dumbbell-like\", perpendicular \"bud-like\", and aggregated “clump-like” hydroxyapatite (HA) crystals, respectively. CS-AM-AMTN, CS-AM-AMBN-C, and CS-AMTN-AMBN-C led to the formation of perpendicular \"dumbbell-like\" and \"bud-like\", parallel \"clump-like\" and \"fine-rod-like\", and vertical \"clump-like\" and \"fine-rod-like\" HA crystals, respectively. CS-AM-AMTN-AMBN-C induced the formation of optimally shaped and aligned HA crystals, characterized by \"rod-like\" crystal bundles of uniform diameter, perpendicular to the enamel surface.</div></div><div><h3>Conclusions</h3><div>AM functions as a mineralization template, promoting the formation of HA crystal bundles. AMBN-C influences the morphology of crystal bundles, while AMTN regulates their arrangement, ultimately facilitating a mineralized surface that resembles natural enamel.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106379"},"PeriodicalIF":2.1,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developmental defects of enamel in preschool twins – Coincidences, heritability, and multilevel analysis","authors":"Francisca Aline da Silva Matias-Santos ,&nbsp;Letícia Caminha Aguiar Lopes ,&nbsp;Maria Eduarda Matos Sousa ,&nbsp;Viviane Oliveira do Nascimento ,&nbsp;Marcoeli Silva de Moura ,&nbsp;Cacilda Castelo Branco Lima ,&nbsp;Fausto Medeiros Mendes ,&nbsp;Lúcia de Fátima Almeida de Deus Moura ,&nbsp;Marina de Deus Moura de Lima","doi":"10.1016/j.archoralbio.2025.106375","DOIUrl":"10.1016/j.archoralbio.2025.106375","url":null,"abstract":"<div><h3>Objective</h3><div>To compare the coincidences of developmental defects of enamel (DDE) between monozygotic and dizygotic twins, determine heritability in primary dentition, and identify associated factors.</div></div><div><h3>Design</h3><div>A cross-sectional study was conducted among preschool twins aged 3–5 years in Teresina, Brazil. Non-cooperative children were excluded. Data were collected through questionnaires and clinical examinations. Clinical assessment was conducted in a school setting using the modified DDE Index for diagnostic purposes. Coincidences were analyzed using tetrachoric correlations and multinomial logistic regression, deriving Odds Ratios (OR) and 95 % confidence intervals (95 %CI). Heritability was estimated using Holzinger’s formula. Multilevel Poisson regression identified associated factors with developmental defects of enamel occurrence (p &lt; 0.05).</div></div><div><h3>Results</h3><div>In total, 239 twin pairs were included (Monozygotic = 96, Dizygotic = 143). Correlations for developmental defects of enamel were strong/very strong in monozygotic (overall DDE, 0.829; demarcated opacities, 0.645; diffuse opacities, 0.812; hypoplasia, 0.786) and weak/moderate in dizygotic (overall DDE, 0.322; demarcated opacities, 0.076; diffuse opacities, 0.562; hypoplasia, 0.156). Monozygotic twins had more positive (OR; 95 %CI = 2.04; 1.01–4.08) and negative coincidences (OR; 95 %CI = 3.91; 1.47–10.36) than dizygotic twins did. Heritability was 74.8 %. Pre-eclampsia/eclampsia and high fever during the first year of life were associated with a higher prevalence of DDE in primary dentition.</div></div><div><h3>Conclusions</h3><div>Higher coincidences in monozygotic twins and high heritability suggest a genetic influence on the development of developmental defects of enamel in primary dentition. Pre-eclampsia/eclampsia and high fever in the first year of life were associated factors.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106375"},"PeriodicalIF":2.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mitophagy attenuates pyroptosis in human gingival fibroblasts through inhibition of NLRP3 inflammasome activation","authors":"Chun-Sheng Bi ,&nbsp;Yi-Kuan Wu ,&nbsp;Hao-Yu Li ,&nbsp;Zi-Jian Cheng ,&nbsp;Liaqat Hussain ,&nbsp;Xiao-Jun Li","doi":"10.1016/j.archoralbio.2025.106378","DOIUrl":"10.1016/j.archoralbio.2025.106378","url":null,"abstract":"<div><h3>Background</h3><div>NLRP3 inflammasome-mediated pyroptosis in gingival fibroblasts (GFs) plays a pivotal role in periodontitis pathogenesis. Mitochondrial dysfunction serves as a critical upstream trigger for NLRP3 inflammasome activation, while mitophagy acts as a key homeostatic mechanism. The regulatory mechanisms of mitophagy in modulating GF pyroptosis remain poorly defined.</div></div><div><h3>Methods</h3><div>Human GFs were used in this study. An <em>in-vitro</em> inflammatory environment was created using 5 μg/mL lipopolysaccharide (LPS). Mitophagy was either activated using P62-mediated mitophagy inducer (PMI, 10 μM) or inhibited with Mdivi-1 (10 μM) in LPS-stimulated and healthy GFs, respectively. Mitophagy was visualized by immunofluorescence, alongside quantification by qRT-PCR/Western blot analysis of proteins associated with mitophagy (PINK1, Parkin, and Beclin-1). Mitochondrial integrity was comprehensively assessed by transmission electron microscopy (TEM), mitochondrial membrane potential (MMP) fluorescence assays, intracellular ROS/mtROS quantification via flow cytometry, and mitochondrial DNA (mtDNA) copy number analysis. NLRP3 inflammasome activation was analyzed by qRT-PCR and Western blot. Pyroptotic cell death was determined by propidium iodide (PI) staining, LDH release, and IL-1β/IL-18 secretion assays.</div></div><div><h3>Results</h3><div>LPS stimulation suppressed mitophagy in GFs, which was effectively rescued by PMI treatment. In contrast, the mitophagy inhibitor decreased basal mitophagy in healthy GFs. PMI-mediated mitophagy enhancement restored mitochondrial function in LPS-exposed GFs, as demonstrated by improved MMP, reduced ROS/mtROS levels, and normalized mtDNA/nDNA ratios. Mechanistically, mitophagy activation attenuated LPS-induced NLRP3 inflammasome assembly, thereby reducing pyroptotic cell death and IL-1β/IL-18 secretion.</div></div><div><h3>Conclusions</h3><div>These findings revealed that mitophagy safeguarded against NLRP3 inflammasome-dependent pyroptosis in GFs by preserving mitochondrial homeostasis, highlighting its therapeutic potential for periodontitis management.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106378"},"PeriodicalIF":2.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CAFs-secreted fatty acids fuel oral cancer progression via lipid raft formation","authors":"Jingtian Mu ,&nbsp;Tingpei Ye ,&nbsp;Junjiang Liu ,&nbsp;Shimeng Wang ,&nbsp;Hongmei Zhou ,&nbsp;Fanglong Wu","doi":"10.1016/j.archoralbio.2025.106377","DOIUrl":"10.1016/j.archoralbio.2025.106377","url":null,"abstract":"<div><h3>Objective</h3><div>Cancer-associated fibroblasts (CAFs) actively reshape the tumor metabolic landscape by synthesizing and secreting free fatty acids (FFAs) that fuel cancer cells malignancy. FFAs are not only catabolized for energy but also incorporated into plasma membrane structures. However, whether cancer cells exploit CAF-derived FFAs for lipid raft assembly—and if this contributes to oncogenic signaling and malignant behaviors in oral cancer—remains largely unexplored.</div></div><div><h3>Design</h3><div>Integrated TCGA and scRNA-seq analyses delineated lipid metabolism characteristics in oral squamous cell carcinoma (OSCC). Immunohistochemistry, immunoblotting, FFAs quantification, and immunofluorescence were used to assess OSCC uptake of CAFs-derived FFAs and lipid raft assembly. Transwell, wound healing, and CCK-8 assays evaluated oncological behaviors. Methyl-β-cyclodextrin (MβCD) and immunoblotting were used to investigate PI3K/AKT pathway activation.</div></div><div><h3>Results</h3><div>Lipogenic enzymes showed gradually increased expression from normal tissue to oral leukoplakia and OSCC. Lipid metabolism reprogramming in CAFs led to abundant FFAs secretion, which enhanced Cav-1 expression and lipid raft formation in OSCC cells. Paracrine FFAs uptake activated PI3K/AKT signaling, promoting proliferation, migration, and invasion. MβCD disrupted lipid rafts and suppressed PI3K/AKT signaling in OSCC cells.</div></div><div><h3>Conclusion</h3><div>CAFs-derived FFAs promote lipid raft synthesis in OSCC cells, activating PI3K/AKT signaling to drive malignant behaviors. Targeting this CAFs–lipid raft axis may represent a novel therapeutic strategy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106377"},"PeriodicalIF":2.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144866250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of adding soluble calcium and pyrophosphate to fluoride toothpaste on enamel remineralization: An in vitro study","authors":"Masashi Fujiki,&nbsp;Mitsuo Kimura,&nbsp;Kei Kurita","doi":"10.1016/j.archoralbio.2025.106376","DOIUrl":"10.1016/j.archoralbio.2025.106376","url":null,"abstract":"<div><h3>Objectives</h3><div>To compare the remineralization ability of a fluoride, soluble calcium, and pyrophosphate (FCaP) toothpaste with that of a fluoride-only toothpaste.</div></div><div><h3>Design</h3><div>Three toothpastes with identical base compositions were prepared: 1450 ppm fluoride (F = 76 µmol/g), 1450 ppm FCaP (F = 76 µmol/g, Ca = 23 µmol/g, P = 23 µmol/g), and 5000 ppm fluoride (F = 263 µmol/g). Soluble fluoride and calcium levels were evaluated by diluting each toothpaste with water (100 mg/10 mL). Bovine enamel specimens with artificially induced subsurface lesions underwent a 21-day pH cycling regimen, with continuous remineralization involving daily 2-hour demineralization and 3-minute toothpaste treatment. Post-pH cycling, quantitative light-induced fluorescence measurement, microhardness testing, and elemental composition analysis were performed (n = 10).</div></div><div><h3>Results</h3><div>The 1450 ppm FCaP toothpaste retained over 95 % soluble fluoride and 30 % soluble calcium. pH cycling test results revealed significantly higher remineralization and fluoride penetration in the 1450 ppm FCaP group than those in the 1450 ppm fluoride-only group (p &lt; 0.05, Steel–Dwass multiple comparison test). No significant differences in enamel calcium-to-phosphorus ratios were observed among the three toothpastes. Interestingly, the 5000 ppm fluoride-only group showed significantly higher fluoride penetration but no improvement in remineralization than that in the other two groups.</div></div><div><h3>Conclusions</h3><div>The 1450 ppm FCaP toothpaste enhanced enamel remineralization more effectively than the fluoride-only toothpaste did. Clinically, FCaP toothpaste can help control dental caries, especially in individuals with low salivary calcium levels.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106376"},"PeriodicalIF":2.1,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144865716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}