Immunohistochemical assessment of murine double minute 2 in solid vs unicystic ameloblastoma: A systematic review and meta-analysis

IF 2.1 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Lilibeth-Stephania Escoto-Vasquez , Mario Alberto Alarcón-Sánchez , Julieta Sarai Becerra-Ruiz , Cristina Hermila Martínez-Bugarin , Sarah Monserrat Lomelí-Martínez , Armen A. Muradyan , Artak Heboyan
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引用次数: 0

Abstract

Objective

This project aimed to evaluate the immunoexpression pattern of murine double minute 2 (MDM2) in solid ameloblastomas compared to unicystic ameloblastomas.

Methods

The review followed PRISMA guidelines and was registered in the PROSPERO database. PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar were comprehensively searched. Original cross-sectional studies were included. The meta-analysis was performed using STATA V15 and RevMan. Positivity rates were pooled using a random-effects model (REM), the labeling index was analyzed using mean difference under a REM, and expression intensity (moderate–strong) was assessed as categorical data using a REM with Hartung-Knapp adjustment. Heterogeneity was evaluated with the Chi² test and I² statistic. The methodological quality and certainty of the evidence were assessed using the Joanna Briggs Institute items and the GRADE system.

Results

Nine studies (n = 438 specimens) were analyzed, of which 325/438 (74.2 %) were ameloblastoma biopsies. MDM2 positivity was detected in 403/438 cases (92 %). A statistically significant association in favor of solid ameloblastoma was observed (RR = 2.08; 95 %CI [1.66–2.60]; p = 0.005), indicating a twofold probability of finding high MDM2 expression in solid compared to unicystic ameloblastomas. Four of the nine studies (44.4 %) were considered to be of low quality, and the certainty of evidence was low to very low.

Conclusions

MDM2 expression was prevalent in both types of ameloblastomas, with a higher intensity of expression observed in solid cases. However, due to study heterogeneity, further investigations with more robust methodological designs are recommended to assess the diagnostic potential of MDM2 in ameloblastomas.
小鼠双分钟2在实体型和单囊型成釉细胞瘤中的免疫组织化学评价:系统回顾和荟萃分析
目的比较小鼠双分钟2 (MDM2)在实体型成釉细胞瘤和单囊型成釉细胞瘤中的免疫表达规律。方法该综述遵循PRISMA指南,并在PROSPERO数据库中注册。对PubMed、Scopus、ScienceDirect、Web of Science和b谷歌Scholar进行了全面检索。纳入了原始的横断面研究。meta分析采用STATA V15和RevMan进行。采用随机效应模型(REM)汇总阳性率,采用REM下的平均差值分析标记指数,采用Hartung-Knapp调整的REM作为分类数据评估表达强度(中-强)。采用Chi²检验和I²统计量评价异质性。使用乔安娜布里格斯研究所项目和GRADE系统评估方法质量和证据的确定性。结果共分析9份研究(n = 438份),其中325/438份(74.2 %)为成釉细胞瘤活检。MDM2阳性403/438例(92 %)。实体成釉细胞瘤与MDM2的相关性有统计学意义(RR = 2.08; 95 %CI [1.66-2.60]; p = 0.005),表明在实体成釉细胞瘤中发现MDM2高表达的概率是单囊性成釉细胞瘤的两倍。9项研究中有4项(44.4% %)被认为是低质量的,证据的确定性从低到非常低。结论smdm2在两种类型的成釉细胞瘤中均有表达,在实性病例中表达强度更高。然而,由于研究的异质性,建议采用更可靠的方法学设计进行进一步的研究,以评估MDM2在成釉细胞瘤中的诊断潜力。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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