Archives of oral biology最新文献

{"title":"Editorial on the special issue on temporomandibular disorders (TMD), AOB journal","authors":"Meiqing Wang , Dongmei He","doi":"10.1016/j.archoralbio.2025.106401","DOIUrl":"10.1016/j.archoralbio.2025.106401","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106401"},"PeriodicalIF":2.1,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effect of cinnamaldehyde in a peri-implant mucositis model","authors":"Verônica Cabral dos Santos Cunha D'Assunção ,&nbsp;Maria Heloísa de Souza Borges-Grisi ,&nbsp;Isis Morais Bezerra Muniz ,&nbsp;Francisco Naldo Gomes Filho ,&nbsp;Camilla Freire de Brito Bastos ,&nbsp;Luís Fellipe Alves Silva ,&nbsp;Francisco Humberto Xavier-Júnior ,&nbsp;Rodrigo Othávio de Assunção e Souza ,&nbsp;Leopoldina de Fátima Dantas de Almeida","doi":"10.1016/j.archoralbio.2025.106397","DOIUrl":"10.1016/j.archoralbio.2025.106397","url":null,"abstract":"<div><h3>Objective</h3><div>Evaluate the antimicrobial effect of an experimental mouthwash containing 1 % cinnamaldehyde in a polymicrobial biofilm model for peri-implant mucositis.</div></div><div><h3>Materials and methods</h3><div>Polymicrobial biofilms were seeded on zirconia and on titanium (n = 6 or 8/group) surfaces from stimulated saliva collection from 2 periodontal health donors and 2 with gingivitis. Salivary pellicle was performed, by 60 min. Inoculum was seeded (1 ×10⁸ CFU/mL) in McBain medium (1 % of sucrose). Biofilms on zirconia were cultured in aerobiosis and on titanium in microaerophilic conditions for 24 h. Daily challenges with 10 % sucrose were stablished. Samples were exposed to the experimental mouthwash for 1 min, twice a day, for 72 h. Chlorhexidine digluconate at 0.12 % and saline solution (at 0.9 %) were used as controls. Biofilms were kept in culture for an additional 24 h to count viable cells, evaluate biomass and cellular metabolism.</div></div><div><h3>Results</h3><div>In zirconia, evaluation of cellular metabolism did not detect a statistically significant difference between the biofilm conditions (p &gt; 0.05). It was found that cellular metabolism was decreased from exposure to mouthwash and chlorhexidine, with a difference compared to the negative control (p &lt; 0.05). In titanium, viable cell count in two media and the biomass were higher for biofilms from the periodontal health salivary condition (p &lt; 0.05). There was a decrease in the biomass of biofilms exposed to the experimental mouthwash for both salivary conditions. Metabolic activity was decreased when exposed to experimental mouthwash and chlorhexidine compared to the negative control (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Experimental mouthwash with 1 % cinnamaldehyde showed an antimicrobial effect similar to chlorhexidine against polymicrobial biofilms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106397"},"PeriodicalIF":2.1,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Triggering mitotic catastrophe by podophyllotoxin induces apoptosis in oral squamous cell carcinoma","authors":"Su-Jung Choi ,&nbsp;Ji-Hoon Kim ,&nbsp;Hyun-Ji Kim ,&nbsp;Dong-Guk Park ,&nbsp;Bohwan Jin ,&nbsp;Won Woo Lee ,&nbsp;Kyoung-Ok Hong ,&nbsp;Sak Lee ,&nbsp;Thantrira Porntaveetus ,&nbsp;Jae-Jin Cho ,&nbsp;Seong-Doo Hong ,&nbsp;Sung-Dae Cho","doi":"10.1016/j.archoralbio.2025.106396","DOIUrl":"10.1016/j.archoralbio.2025.106396","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the relationship between mitotic catastrophe (MC) and apoptosis in oral squamous cell carcinoma (OSCC) using podophyllotoxin (PPT), a natural compound with antimitotic properties.</div></div><div><h3>Design</h3><div>We evaluated the concentration-dependent effects of PPT on cell proliferation (CCK-8 and soft agar assays) and morphology (transmission electron microscopy). Mechanistic insights were obtained by assessing DNA damage (western blotting), cell cycle progression (sub-G₁ analysis), and apoptosis-related protein activation in both 2D and 3D spheroid models of HSC-3 oral squamous carcinoma cells.</div></div><div><h3>Results</h3><div>PPT exerted pronounced inhibitory effects on cell proliferation and anchorage-independent growth accompanied by morphological indications of MC, such as enlarged multinucleated cells. DNA damage induced by PPT resulted in ataxia telangiectasia mutated kinase and checkpoint kinase 2 activation, leading to G₂/M arrest and cyclin B1upregulation. Importantly, PPT-induced MC was followed by apoptosis, as evidenced by an increased sub-G₁ population, Annexin V positivity, and caspase activation. Mitochondrial dysfunction, as indicated by altered membrane potential and enhanced Bax expression, underscored the apoptotic process. Caspase-2 activation emerged as a pivotal event, cleaving Bid and establishing a link between MC and the intrinsic apoptotic pathway. The effects were consistent across both 2D and 3D models, suggesting a robust therapeutic potential.</div></div><div><h3>Conclusions</h3><div>This study provides compelling evidence supporting the potential therapeutic significance of inducing MC-mediated apoptosis in OSCC. The results underscore the role of PPT and its derivatives, such as etoposide and teniposide, in targeting rapidly dividing cancer cells through interference with mitotic progression, offering insights into novel therapeutic strategies for oral cancer.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106396"},"PeriodicalIF":2.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective effect of a novel antioxidant gel containing resveratrol and sodium fluoride on dentin erosion in the presence of acquired salivary pellicle: An in vitro study","authors":"Loraine Perez Manzoli ,&nbsp;Luan Júlio Ruiz da Silva ,&nbsp;George Clay dos Santos Caracas ,&nbsp;Kalinca Furtado de Oliveira ,&nbsp;Walessa Alana Braganca Aragão ,&nbsp;Rafael Rodrigues Lima ,&nbsp;Milton Carlos Kuga ,&nbsp;Cristiane de Melo Alencar","doi":"10.1016/j.archoralbio.2025.106395","DOIUrl":"10.1016/j.archoralbio.2025.106395","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the protective effect of experimental gels containing resveratrol, with or without 1.23 % sodium fluoride (NaF), and their interaction with the salivary pellicle on dentin erosion.</div></div><div><h3>Design</h3><div>Gels with 5 % or 10 % resveratrol (w/w) were prepared, with NaF added at 1.23 % (w/w). Total polyphenol content (TPC) and antioxidant capacity (ORAC) were determined. Human dentin specimens (n = 210) were allocated into seven groups (n = 30): negative control, NaF (1.23 %), 5 % resveratrol, 10 % resveratrol, 5 % resveratrol + NaF, 10 % resveratrol + NaF, and positive control (commercial anti-erosion toothpaste). Each group was divided according to the presence or absence of salivary pellicle (n = 15). After gel application, specimens were submitted to a 7-day erosive–abrasive cycling protocol. Outcomes included surface loss (3D CLM), collagen degradation after collagenase, calcium release (atomic absorption spectrometry), and tubule obliteration (CLM). Data were analyzed using two-way ANOVA and Tukey’s test (α = 0.05).</div></div><div><h3>Results</h3><div>The 10 % resveratrol gel had the highest TPC and ORAC (p = 0.03), followed by 10 % resveratrol + NaF (p = 0.013), whereas 5 % resveratrol showed lower values (p = 0.021). The salivary pellicle significantly reduced dSL-eroded, dColl, and CaR in all groups (p = 0.001). Combined formulations showed the greatest protection: G5 and G6 had lower dSL-eroded (p = 0.002, 0.004), dColl (p = 0.014, 0.021), and dSL-total (p = 0.007, 0.008), with G6 showing the lowest CaR (p = 0.023).</div></div><div><h3>Conclusion</h3><div>The combination of 10 % resveratrol and NaF effectively protected against dentin erosion, with salivary pellicle enhancing these effects.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106395"},"PeriodicalIF":2.1,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endocannabinoid system in periodontitis: A systematic review and in silico analyses","authors":"Lélio Fernando Ferreira Soares ,&nbsp;Jovânia Alves Oliveira ,&nbsp;Andressa Vilas Boas Nogueira ,&nbsp;Carlos Rossa Júnior ,&nbsp;Suzane Cristina Pigossi ,&nbsp;James Deschner ,&nbsp;Joni Augusto Cirelli","doi":"10.1016/j.archoralbio.2025.106394","DOIUrl":"10.1016/j.archoralbio.2025.106394","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review with in silico investigation discusses the involvement of the endocannabinoid system (ECS), particularly CB1 and CB2 receptors (genes <em>CNR1</em> and <em>CNR2</em> respectively), in periodontal health and disease (PD).</div></div><div><h3>Methods</h3><div>PubMed/MEDLINE, Embase, Web of Science, Scopus, and The Cochrane Library databases were searched for studies on periodontitis and ECS published up to August 2024. The GSE16134 dataset was used for analyses of differential gene expression, correlation of ECS genes, evaluation of biomarkers and functional enrichment analysis.</div></div><div><h3>Results</h3><div>Nine studies met the inclusion criteria (three clinical and six preclinical studies). Clinical studies demonstrated that <em>CNR2</em> gene expression was significantly reduced in periodontitis, while <em>CNR1</em> showed minor changes. Animal studies with CB2 activation by different therapies increased receptor expression, reduced pro-inflammatory cytokines, and mitigated alveolar bone loss. CB1 activation also reduced inflammation and bone loss. Anandamide (AEA), an endogenous ligand of the cannabinoid receptors, exhibited anti-inflammatory effects, with endogenous levels decreasing after therapy. Bioinformatics analysis revealed that <em>CNR1</em> expression in PD tissues was positively associated with genes involved in B-cell activation and humoral immune responses. In contrast, <em>CNR2</em> expression showed strong correlations with genes related to immune regulation and extracellular matrix remodeling, suggesting distinct yet complementary roles for CB1 and CB2 in periodontal inflammation.</div></div><div><h3>Conclusions</h3><div>The ECS participates in periodontal inflammation, with CB2 activation emerging as a promising therapeutic target.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106394"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145088214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of antibacterial and antibiofilm potential of chemically- and green laser-produced gold and silver nanoparticles against dental pathogens","authors":"Milica Nemoda ,&nbsp;Marija Radovanović ,&nbsp;Sanja Živković ,&nbsp;Miloš Momčilović ,&nbsp;Vladimir Rajić ,&nbsp;Marko Bošković ,&nbsp;Biljana Nikolić ,&nbsp;Jelena Marinković","doi":"10.1016/j.archoralbio.2025.106393","DOIUrl":"10.1016/j.archoralbio.2025.106393","url":null,"abstract":"<div><h3>Objectives</h3><div>This study compared antibacterial and antibiofilm effectiveness of chemically (chem_) and pulsed laser ablation-synthesized (laser_) gold (Au) and silver (Ag) nanoparticles (NPs), namely chem_AuNPs, chem_AgNPs, laser_AuNPs, and laser_AgNPs, against four <em>Streptococcus</em> species (<em>S. mutans, S. mitis, S. sanguinis,</em> and <em>S. gordonii</em>), essential for cariogenic biofilm development.</div></div><div><h3>Design</h3><div>Nanoparticles were characterized for size and stability using dynamic light scattering (DLS) and transmission electron microscopy (TEM). Their antibacterial and antibiofilm activities were tested in microdilution and crystal violet assays, respectively. <em>Ex vivo</em> evaluation of the nanoparticles antibiofilm activity was performed against a four-species biofilm on enamel discs by quantifying CFU. Scanning electron microscopy was used to visualize the reduction in biofilm mass on enamel discs treated with laser_Au and Ag nanoparticles.</div></div><div><h3>Results</h3><div>TEM showed nanoparticle sizes ranging 5.89–18.49 nm. DLS revealed the stability of NPs colloid solutions, with ζ-potential approximately −30 mV over 21 days. All NPs exhibited strong antibacterial activity, with MIC values of 56.9–120.6 μg mL-1 for chem_AuNPs and 1.9–30.8 μg mL-1 for all the rest. The evaluation of NPs potential in biofilm prevention showed the following trend in activity: laser_AuNPs&gt;laser_AgNPs&gt;chem_AuNPs&gt;chem_AgNPs. AuNPs, regardless of synthesis method, were as effective as the positive control (chlorhexidine-mouthwash) against the multispecies biofilm settled on enamel discs, while AgNPs were nearly twice as efficient.</div></div><div><h3>Conclusions</h3><div>AuNPs and AgNPs could be alternatives for managing cariogenic bacteria both planktonic and within biofilms. Laser_AgNPs, being more efficient than commercial mouthwash, seem to be the most valuable candidate.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106393"},"PeriodicalIF":2.1,"publicationDate":"2025-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive caries-preventing effects of mineral ions released from surface pre-reacted glass-ionomer (S-PRG) filler on oral biofilm","authors":"Kazuo Kato ,&nbsp;Ryo Kutsuna ,&nbsp;Yoshiaki Kawamura ,&nbsp;Yoshihiro Shimazaki","doi":"10.1016/j.archoralbio.2025.106392","DOIUrl":"10.1016/j.archoralbio.2025.106392","url":null,"abstract":"<div><h3>Objective</h3><div>The bioactive caries-preventing effects of mineral ions released from surface pre-reacted glass-ionomer (S-PRG) filler on oral biofilm were estimated by analyzing the depth-specific density of <em>Streptococcus mutans</em> and <em>Streptococcus sanguinis</em>.</div></div><div><h3>Design</h3><div>Ten participants wore <em>in situ</em> plaque-generating enamel slab devices on their upper molars to form biofilm for 5 days. Filtrates were prepared from prophylaxis paste slurries containing S-PRG filler, whereas paste without the filler served as the control. The devices were alternately immersed in sucrose solution three times and in the experimental or control filtrate twice each day. Two biofilm samples taken from each device were separated into 6–9 layered fractions (100 μm each) by serial sectioning. One sample was used for extraction of genomic DNA. The gene sequences encoding D-alanine:D-alanine ligases were amplified using universal and species-specific primers. Borate, aluminum, silicate, and strontium concentrations were determined using thicker sections of the other sample, correcting for biomass volume estimated by the area measurement of stained thinner sections.</div></div><div><h3>Results</h3><div>The ratios of <em>S. mutans</em> to <em>S. sanguinis</em> in the outer, middle, and inner layers were 0.0359, 0.0254 and 0.0157 for the experimental biofilm and 0.046, 0.0325 and 0.0255 for control biofilm, respectively. The ratio in the whole layer was significantly lower in the experimental biofilm than control biofilm. Strontium and aluminum concentrations in the experimental biofilm were 114- to 146-fold and 2.74- to 4.48-fold higher compared with control biofilm. respectively.</div></div><div><h3>Conclusions</h3><div>These results suggest that S-PRG filler has bioactive caries-preventing effects on oral biofilm.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106392"},"PeriodicalIF":2.1,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective bacterial proteolysis in periodontal disease: Molecular mechanisms and therapeutic implications, a narrative review","authors":"Andrea Escalante-Herrera,&nbsp;Walter L. Siqueira","doi":"10.1016/j.archoralbio.2025.106391","DOIUrl":"10.1016/j.archoralbio.2025.106391","url":null,"abstract":"<div><h3>Background/Objective</h3><div>Periodontal diseases are characterized by complex interactions between the immune system, oral bacteria, host tissues, and various external and internal factors. A hallmark of disease progression is the selective degradation of host proteins by bacterial proteases. This review explores the molecular mechanisms of bacterial proteolysis, with a focus on the selectivity toward host proteins essential for oral homeostasis.</div></div><div><h3>Methods</h3><div>An extensive electronic search was conducted using PubMed, Scopus, and Web of Science, covering the last 20 years. Search terms included “degradation,” “oral tissue,” “proteins,” “bacteria,” “periodontal disease,” “periodontitis,” “pathways,” and “host.” Boolean operators and filters were applied to refine results. Articles were evaluated based on titles, abstracts, and full texts. Additional references were identified through citation tracking. A narrative synthesis approach was used to integrate findings.</div></div><div><h3>Results</h3><div>Bacterial proteases exhibit precise substrate recognition, targeting specific structural, immune, and signaling proteins in the oral environment. The proteolytic profile and disease pathology are shaped by microbial community dynamics, including cooperative and competitive interactions. Host factors such as genetic diversity, local microenvironmental conditions, and immune responses further modulate protein degradation. Despite advancements, critical gaps remain, particularly regarding post-translational modifications and the determinants of proteolytic specificity.</div></div><div><h3>Conclusions</h3><div>Selective bacterial proteolysis plays a pivotal role in periodontal disease pathogenesis. Addressing current knowledge gaps through advanced proteomics, longitudinal studies, and imaging technologies is essential for developing targeted diagnostic and therapeutic strategies. An integrative approach is needed to enhance periodontal disease management and improve patient outcomes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106391"},"PeriodicalIF":2.1,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probiotic supplementation attenuates dental pain and inhibits pain-induced cognitive impairment in male rats","authors":"Pegah Abazari-Bozhgani ,&nbsp;Saeed Esmaeili-Mahani ,&nbsp;Mehdi Abbasnejad ,&nbsp;Maryam Raoof ,&nbsp;Frank Lobbezoo","doi":"10.1016/j.archoralbio.2025.106382","DOIUrl":"10.1016/j.archoralbio.2025.106382","url":null,"abstract":"<div><h3>Objectives</h3><div>The gut-brain axis has emerged as a promising avenue for understanding the bidirectional communication between the gut microbiota and the central nervous system. This study investigated the potential impact of probiotics, <em>Lactobacillus acidophilus</em> (LA-5), <em>Lactobacillus paracasei</em> (<em>L. casei</em> 431), and <em>Bifidobacterium lactis</em> (BB-12), as well as their combination, on dental pulp pain management and cognitive functions.</div></div><div><h3>Design</h3><div>Forty-eight male Wistar rats were randomly assigned to six experimental groups (n = 8). The probiotics (10⁹ CFU) were orally administered for 14 consecutive days. Capsaicin (100 µg) was used to induce inflammatory pulp nociception. The Morris water maze task was used to evaluate learning and memory performance. Levels of the interleukin-1 beta (IL-1β) and tumour necrosis factor-alpha (TNF-α) cytokines in the animals' trigeminal ganglion (TG) and the brain-derived neurotrophic factor (BDNF), neuropeptide Y (NPY), tropomyosin receptor kinase B (Trk-B) and Cyclooxygenase-2 (COX-2) genes in the animals' hippocampus were determined using western blotting and RT-PCR, respectively.</div></div><div><h3>Results</h3><div>Intradental application of capsaicin induced nociceptive behavior and increased IL-1β and TNF-α in the TG of rats. Probiotics could attenuate nociception and prevent IL-1β and TNF-α overexpression. Furthermore, pain induced cognitive impairments, decreased BDNF, NPY, and Trk-B and increased COX-2 gene expression in rat hippocampus, which were inhibited by probiotics supplementation.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that probiotics may play a role in orofacial pain relief and cognitive enhancement in painful situations by modulating gut microbiota composition and influencing protein levels and gene expression in brain regions associated with pain and cognition.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106382"},"PeriodicalIF":2.1,"publicationDate":"2025-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of parecoxib and dexamethasone on the temporomandibular joint of orchiectomized rats: Morphological and immunological analysis","authors":"Victor Augusto Benedicto dos Santos ,&nbsp;Francisco Carlos Groppo ,&nbsp;José Roberto Garcia de Toledo ,&nbsp;Michael Henrique Araújo Monteiro ,&nbsp;Guilherme Elias Pessanha Henriques ,&nbsp;Sidney Raimundo Figueroba","doi":"10.1016/j.archoralbio.2025.106381","DOIUrl":"10.1016/j.archoralbio.2025.106381","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of parecoxib (a selective COX-2 inhibitor) and dexamethasone (a corticosteroid) on the temporomandibular joint (TMJ) of orchiectomized rats, a model of testosterone deficiency, through histological and immunological analyses.</div></div><div><h3>Methods</h3><div>Thirty-six rats were divided into six groups (n = 6). Sham groups received saline, parecoxib (0.3 mg/kg), or dexamethasone (0.1 mg/kg). ORX groups received the same treatments. TMJs were processed for histological staining (toluidine blue and picrosirius red) and analyzed by histomorphometry, measuring total cartilage thickness and its layers (fibrous, proliferative, mature, hypertrophic). Cytokines (IL-1α, IL-1β, IL-6, TNF-α) were quantified by ELISA. Data were analyzed using ANOVA-Welch and Kruskal-Wallis tests.</div></div><div><h3>Results</h3><div>ORX increased IL-1α, IL-1β, and TNF-α levels. IL-6 was reduced by dexamethasone. Dexamethasone also decreased cartilage thickness and accelerated its differentiation into subchondral bone. In contrast, parecoxib preserved cartilage thickness, especially in the fibrous and proliferative layers, and increased proteoglycan content. Both drugs reduced inflammatory markers, but with distinct structural effects.</div></div><div><h3>Conclusions</h3><div>Testosterone deficiency enhanced TMJ inflammation and impaired cartilage structure. While both dexamethasone and parecoxib modulated these effects, their actions differed: dexamethasone promoted cartilage-to-bone differentiation, potentially unfavorable long term, whereas parecoxib preserved cartilage integrity. These findings underscore hormonal influence and support selective anti-inflammatory strategies for TMJ preservation under androgen deficiency.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106381"},"PeriodicalIF":2.1,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144891908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}