Archives of oral biology最新文献

{"title":"Phellodendron bark extract and berberine chloride suppress microbiome dysbiosis in a saliva-derived in vitro microcosm biofilm model","authors":"Takuya Inokuchi ,&nbsp;Kiyoshi Tomiyama ,&nbsp;Takuma Okuda ,&nbsp;Kota Tsutsumi ,&nbsp;Kazuma Yama ,&nbsp;Yuto Fujii ,&nbsp;Kanta Ohara ,&nbsp;Takashi Chikazawa ,&nbsp;Yasushi Kakizawa ,&nbsp;Yoshiharu Mukai","doi":"10.1016/j.archoralbio.2025.106231","DOIUrl":"10.1016/j.archoralbio.2025.106231","url":null,"abstract":"<div><h3>Objective</h3><div>Preventing oral microbiome dysbiosis is crucial for averting the onset and progression of periodontal diseases. <em>Phellodendron</em> bark extract (PBE) and its active component berberine exhibit antibacterial properties against periodontal pathogenic bacteria. Although they inhibit <em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>)-induced dysbiosis <em>in vitro</em> in multiple species of saliva-derived planktonic cultures, their effects on microcosm biofilm models remain unclear. In this study, we aimed to elucidate the dysbiosis-suppressive effects of PBE and berberine chloride (BC) on biofilm formation.</div></div><div><h3>Design</h3><div>PBE or BC was added during the formation of <em>in vitro</em> microcosm biofilms containing saliva and <em>P. gingivalis</em>, which were anaerobically cultured for one week. Next-generation sequencing was performed to assess microbiota composition, while quantitative real-time PCR was used to measure bacterial concentrations. Additionally, the butyrate concentration in the culture supernatant was assessed as biofilm pathogenicity.</div></div><div><h3>Results</h3><div>PBE and BC treatments reduced the relative abundance of periodontal pathogenic bacteria, including <em>P. gingivalis,</em> and significantly increased the relative abundance of the genus <em>Streptococcus</em> and nitrate-reducing bacteria, including the genera of <em>Neisseria</em> and <em>Haemophilus.</em> Moreover, the treatment groups exhibited significantly decreased butyrate concentrations.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that PBE and BC could suppress dysbiosis triggered by <em>P. gingivalis</em> in microcosm biofilms <em>in vitro</em> by decreasing the relative abundance and amount of periodontal pathogenic bacteria and enhancing those of nitrate-reducing bacteria that have a high relative abundance in orally healthy individuals. In summary, PBE and BC may contribute to the prevention of periodontal disease through their dysbiosis-suppressive and anti-inflammatory effects.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106231"},"PeriodicalIF":2.2,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143808355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High prevalence of oral cavity parasites and its related risk factors in Iranian children with stomatitis; A case-control study","authors":"Behnoush Selahbarzin ,&nbsp;Yosra Raziani ,&nbsp;Azadeh Jafrasteh ,&nbsp;Parastoo Baharvand ,&nbsp;Fatemeh Doulatshah ,&nbsp;Hossein Mahmoudvand ,&nbsp;Fahimeh Kooshki","doi":"10.1016/j.archoralbio.2025.106239","DOIUrl":"10.1016/j.archoralbio.2025.106239","url":null,"abstract":"<div><h3>Objective</h3><div>This study for the first time seeks to assess the prevalence, socio-economic characteristics, and risk factors associated with oral cavity parasites (<em>Entamoeba gingivalis</em> (Eg) and <em>Trichomonas tenax</em> (Tt)) in children with stomatitis in Lorestan province, Western Iran.</div></div><div><h3>Design</h3><div>The present case-control study encompassed a group of 215 children with stomatitis (ST) and 215 children without stomatitis (non-ST) who were referred to healthcare facilities in Lorestan province, Iran, between December 2024 and January 2025. Samples were collected from participants and were analyzed under a light microscope.</div></div><div><h3>Results</h3><div>The overall prevalence of oral parasites in children with ST was determined to be 59 (27.4 %) and 64 (29.8 %) using microscopic examination and PCR techniques, respectively. Among the positive cases, 48 (75.0 %) of the children were found to be positive for Eg, while 16 (25.0 %) tested positive for Tt. The likelihood of encountering oral cavity parasites among ST participants is markedly higher compared to that of the non-ST group (p &lt; 0.001, OR=2.180; CI= 1.369–3.471). In the multivariate analysis, several factors were found to be significantly associated with parasitic infection, including male gender (p = 0.024, OR= 2.173, 95 % CI: 1.107–4.265), high parental education (p = 0.007, OR= 3.138, 95 % CI:1.373–7.170), living in urban regions (p = 0.000, OR=0.259, 95 % CI: 0.123–0.546), and the use of mouthwash (p = 0.000).</div></div><div><h3>Conclusion</h3><div>The research revealed a significant occurrence of oral cavity parasites among children with stomatitis in Lorestan province, Western Iran. Consequently, pediatric dental practitioners should maintain a heightened awareness of these risk factors for reducing the prevalence of oral infections in children.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106239"},"PeriodicalIF":2.2,"publicationDate":"2025-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686318","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensory innervation of rat molar root pulp and dentin, apex, foramen, cellular cementum, vasculature and periodontium","authors":"Margaret R. Byers","doi":"10.1016/j.archoralbio.2025.106238","DOIUrl":"10.1016/j.archoralbio.2025.106238","url":null,"abstract":"<div><h3>Objective</h3><div>Trigeminal innervations of dental roots have only been partly investigated. The aim of this study was to document sensory innervation of rat molar roots, especially their neural patterns in periapical tissues that may affect tooth pain.</div></div><div><h3>Design</h3><div>Trigeminal nerve ending patterns in young and old rat molars that had been labeled by tritiated axonal-transported protein were compared by immunocytochemistry for neural <em>peripherin, calcitonin gene-related peptide, p75-neurotrophin receptor, synaptophysin, Substance P</em>, and <em>neurofilament-200.</em></div></div><div><h3>Results</h3><div>Nine sensory nerve <em>patterns</em> were found in the autoradiograms: (1) PERIAPICAL PULP had <em>large axons ending among odontoblasts</em>, other axons with <em>free endings in pulp</em>, plus <em>innervation of dentin</em> on the palatal-lingual and buccal sides of each root. (2) CELLULAR CEMENTUM MATRIX (CCx) had <em>small nerve channels</em> extending across from periodontium to apex, plus trigeminal <em>endings in the CCx</em> at its apical and periodontal edges. (3) THE FORAMEN REGION had many <em>free axons</em> plus much <em>vascular innervation</em>. (4) THE APEX had a variety of <em>axons ending along its walls</em>. (5) NEARBY PERIODONTIUM had <em>neurovascular clusters</em> including trigeminal axons and Ruffini mechanoreceptors near the foramen. Immunocytochemistry showed that CGRP, peripherin, synaptophysin, and p75-neurotrophin receptor each had individual neural patterns in periapical tissues, while Substance P and neurofilament protein were more limited. Differences between lateral and accessory canals were noted.</div></div><div><h3>Conclusion</h3><div>The nine periapical trigeminal innervation patterns identified so far differ from crown and root shaft. They likely have important effects on tooth pain in general and on periapical pains that can be difficult to anesthetize and treat.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106238"},"PeriodicalIF":2.2,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gallic acid promotes M2 macrophage polarization through mitochondrial oxidative phosphorylation in periodontitis","authors":"Ruobing Zhang ,&nbsp;Wenjing Yang ,&nbsp;Kai Li ,&nbsp;Xiaolong Zhang ,&nbsp;Jie Liu ,&nbsp;Lin Ai","doi":"10.1016/j.archoralbio.2025.106237","DOIUrl":"10.1016/j.archoralbio.2025.106237","url":null,"abstract":"<div><h3>Objective</h3><div>This in vitro investigation assessed the role of gallic acid in regulating macrophage polarization.</div></div><div><h3>Design</h3><div>In this study, RAW264.7 macrophages were treated with <em>P. gingivalis</em> lipopolysaccharide (LPS) to mimic inflammatory conditions in periodontitis. Immunofluorescence staining, enzyme‑linked immunosorbent (ELISA) assay, RNA sequencing, and seahorse metabolic profile assay were used to assess the effects of gallic acid on macrophage polarization, cytokine release, underlying mechanism and metabolic profile.</div></div><div><h3>Results</h3><div>The study demonstrated that treatment with gallic acid could induce M2 polarization of macrophages (<em>P</em> &lt; 0.01) and increase the production of anti-inflammatory cytokines (<em>P</em> &lt; 0.01). Analysis of RNA sequencing data showed enrichment of mitochondrial oxidative phosphorylation, regulation of metabolic processes, and the PI3K-Akt signaling pathway in LPS-treated macrophages treated with gallic acid. Furthermore, gallic acid was found to enhance mitochondrial oxidative phosphorylation activity through the PI3K-Akt signaling pathway in RAW264.7 cells.</div></div><div><h3>Conclusions</h3><div>Gallic acid treatment has the potential to promote M2 polarization of macrophages by modulating mitochondrial oxidative phosphorylation via the PI3K-Akt signaling pathway.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106237"},"PeriodicalIF":2.2,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advance in candidate genes in mandibular retrognathism: A systematic review","authors":"Li Yang ,&nbsp;Weiping Yang ,&nbsp;Yining Shen ,&nbsp;Yu Zhou","doi":"10.1016/j.archoralbio.2025.106234","DOIUrl":"10.1016/j.archoralbio.2025.106234","url":null,"abstract":"<div><h3>Objective</h3><div>This research aims to dissect the polygenic nature of non-syndromic mandibular retrognathism (MR) and to better understand the genetic underpinnings of MR, with a particular focus on the role of ethnic diversity in influencing genetic predispositions.</div></div><div><h3>Methods</h3><div>A comprehensive systematic review was conducted on MR. Electronic databases such as PubMed and Google Scholar were employed, utilizing terms like 'mandibular', 'retrognathism', 'gene', and 'genetic'. This study strictly adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) framework.</div></div><div><h3>Results</h3><div>Ten genetic studies were identified that satisfied the eligibility criteria, involving 1010 participants. Variations in candidate genes were reported across different populations, including myosin 1 H (<em>MYO1H</em>), matrilin 1 (<em>MATN1</em>), a disintegrin and metalloproteinase with thrombospondin motifs 9 (<em>ADAMTS9</em>), bone morphogenetic protein 2 (<em>BMP2</em>), parathyroid hormone (<em>PTH</em>), the vitamin-D related genes: vitamin D receptor (<em>VDR</em>), cytochrome P450 family 24 subfamily A member 1 (<em>CYP24A1</em>), and cytochrome P450 family 27 subfamily B member 1 (<em>CYP27B1</em>), collagen type II alpha 1 chain (<em>COL2A1</em>), transforming growth factor-β (<em>TGF-β</em>), <em>TGF</em>-β receptor 2 (<em>TGFBR2</em>), epidermal growth factor (<em>EGF</em>), and EGF receptor gene (<em>EGFR</em>).</div></div><div><h3>Conclusion</h3><div>These findings shed light on the role of genetic factors in MR. Future studies should adopt a multicentric approach to expand sample sizes and enhance the analysis of genetic variants associated with MR.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106234"},"PeriodicalIF":2.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143686317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single monoiodoacetic acid injection reveals toll-like receptor, oestrogen, oxidative stress, and altered energy metabolism as key drivers of temporomandibular joint osteoarthritis in female rats","authors":"Shasha Liu ,&nbsp;Chaolun Wu ,&nbsp;Yuan Yao ,&nbsp;Xiongwei Dong ,&nbsp;Lili Xu","doi":"10.1016/j.archoralbio.2025.106233","DOIUrl":"10.1016/j.archoralbio.2025.106233","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to establish a reproducible and minimally invasive rat model of temporomandibular joint osteoarthritis (TMJ-OA) using intra-articular monoiodoacetic acid (MIA) injection, and to investigate the pathological mechanisms underlying TMJ-OA development, aimed at providing insights for potential clinical treatments.</div></div><div><h3>Design</h3><div>We compared the effects of single versus multiple MIA injections on body weight, pain behaviour, and condylar pathology in female Sprague–Dawley rats. We longitudinally assessed the progression of TMJ-OA over 5 weeks by evaluating condylar pathology and immunohistochemical staining. We investigated the potential mechanism of MIA-induced TMJ-OA through transcriptome sequencing and polymerase chain reaction validation.</div></div><div><h3>Results</h3><div>A single MIA injection (0.5 mg) into the joint space effectively induced TMJ-OA in rats and sustained inflammatory reactions and pain without significantly affecting weight. MIA continuously promoted the development of TMJ-OA through the activation of the toll-like receptor pathway, oestrogen metabolism promotion, oxidative stress response enhancement, and energy metabolism alteration in condylar chondrocytes.</div></div><div><h3>Conclusion</h3><div>We have presented a simple and minimally invasive method for modelling TMJ-OA in rats, which can be utilised in animal trials focusing on TMJ-OA treatment strategies. The study also reveals toll-like receptor, oestrogen, oxidative stress, and altered energy metabolism as key drivers of TMJ-OA in female rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106233"},"PeriodicalIF":2.2,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of oral conditions to explore subsistence strategies in the ecologically diverse environments of the Canary Islands during the Amazigh period (1st to 15th centuries CE)","authors":"Aarón Morquecho Izquier ,&nbsp;Rebeca García González ,&nbsp;Elías Sánchez Cañadillas ,&nbsp;Jonathan Santana","doi":"10.1016/j.archoralbio.2025.106236","DOIUrl":"10.1016/j.archoralbio.2025.106236","url":null,"abstract":"<div><div>Objective:</div><div>This study examines oral health conditions in the pre-European Amazigh population of the Canary Islands (1st–15th centuries) to uncover patterns linked to island-specific environments and subsistence practices. The Canary Islands offered diverse ecological settings that shaped how the Amazigh adapted their agricultural practices, creating landscapes to support settlement.</div><div>Design:</div><div>By analyzing the oral health of 615 adult individuals across seven major islands—including caries, dental wear, calculus, periodontal disease, and antemortem tooth loss—the study identifies three distinct patterns of oral health within the archipelago.</div><div>Results:</div><div>Gran Canaria exhibited the highest prevalence of dental caries, aligning with an agriculture-centered lifestyle. In contrast, La Gomera, Fuerteventura, and Lanzarote showed the lowest caries rates, suggesting less agricultural dependence. El Hierro and La Palma populations presented both high caries prevalence and extensive dental wear, likely due to a diet including famine-related foods like fern roots. The findings also indicate sex-based differences in oral health across islands but no significant distinctions by age.</div><div>Conclusions:</div><div>These patterns highlight a relationship between the diverse biogeography of the islands, subsistence strategies, and oral health in the Amazigh period. This study contributes valuable insights into how different environments and resource availability impacted the diet and health of the Canary Islands’ Amazigh populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106236"},"PeriodicalIF":2.2,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic polymorphisms on temporomandibular disorders: Network meta-analysis","authors":"Daniel Augusto de Faria Almeida ,&nbsp;Camila Freire Brant ,&nbsp;Letícia da Costa Siqueira ,&nbsp;Lélio Fernando Ferreira Soares ,&nbsp;Jovânia Alves Oliveira ,&nbsp;Daniela Silva Barroso de Oliveira ,&nbsp;Suzane Cristina Pigossi ,&nbsp;Carlos José Soares","doi":"10.1016/j.archoralbio.2025.106235","DOIUrl":"10.1016/j.archoralbio.2025.106235","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this systematic review and network meta-analysis (NMA) is to compare and rank the effects of different genetic polymorphisms on the susceptibility of temporomandibular disorders (TMDs) occurrence.</div></div><div><h3>Design</h3><div>The central question formulated was: \"Are genetic polymorphisms involved in the etiology of TMDs?\" Following PROSPERO registration (CRD42024507886), electronic searches were conducted in five databases for publications up to November 2024.</div></div><div><h3>Results</h3><div>Sixty-three studies were included in the systematic review and 7 composed the NMA. The qualitative analysis summarized the association between 120 genes (and 206 polymorphisms) and TMDs. Thirty-two polymorphisms (in 24 genes) were linked to overall TMDs, while 22 polymorphisms (in 22 genes) with degenerative bone changes in the temporomandibular joint (TMJ). Additionally, 17 polymorphisms were identified in cases of painful chronic TMD, while 12 polymorphisms in intra-articular disorders. These polymorphisms were in genes related to neurotransmission (COMT, ADRB2, DRD2, ANKK1, SLC6A4 and HTR2A), inflammatory mediators (TNFα, IL10 and MMP1), sex hormones (ESR1and ESRRB), oxidative stress (GSTM1) and bone metabolism (VDR). A protective effect for myalgia occurrence with the COMT_rs165774 polymorphism compared to the wild-type genotype was found in the pairwise meta-analysis (AG genotype: OR: 0.33; 95 %CI: 0.14, 0.76; p &lt; 0.01 and GG genotype: OR: 0.32; 95 %CI: 0.14, 0.74; p &lt; 0.01) and this polymorphism showed the highest probability of being associated with the myalgia (97 %) and arthralgia (93 %) conditions.</div></div><div><h3>Conclusions</h3><div>Genetic polymorphisms in genes related to neurotransmission, inflammatory response, and sex hormones seem to be risk factors related to the TMDs pathogenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"174 ","pages":"Article 106235"},"PeriodicalIF":2.2,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143642955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Shear stress preconditioning enhances periodontal ligament stem cell survival","authors":"Ravipha Suwittayarak ,&nbsp;Nuttha Klincumhom ,&nbsp;Chaloemrit Phrueksotsai ,&nbsp;Nuttapol Limjeerajarus ,&nbsp;Chalida Nakalekha Limjeerajarus ,&nbsp;Hiroshi Egusa ,&nbsp;Thanaphum Osathanon","doi":"10.1016/j.archoralbio.2025.106232","DOIUrl":"10.1016/j.archoralbio.2025.106232","url":null,"abstract":"<div><h3>Objective</h3><div>The study investigated <em>in vitro</em> the influences of shear stress preconditioning on human periodontal ligament stem cells (hPDLSCs) under serum deprivation.</div></div><div><h3>Design</h3><div>hPDLSCs were subjected to shear stress at 0.5 and 5 dyn/cm², both with and without serum starvation. Cell viability and apoptosis were assessed using the Resazurin assay and flow cytometry analysis, respectively. Gene and protein expressions were analysed by real-time polymerase chain reaction, immunofluorescent staining, and Western blotting.</div></div><div><h3>Results</h3><div>Our results revealed that shear stress potentially mitigated serum derivation-induced cell death by inducing cell viability, enhancing colony formation, and inhibiting cell apoptosis. The addition of an ERK inhibitor inhibited the shear stress-induced cell apoptosis resistance. Shear stress treatment upregulated cell viability-related gene expression, including <em>SOX2</em>, <em>SOD1</em> and <em>BIRC5.</em> In particular, shear stress promoted the nuclear translocation of SOX2. Meanwhile, the expression of <em>BIRC5</em> was not inhibited by cycloheximide. Shear stress-induced <em>SOX2</em> and <em>BIRC5</em> expression was attenuated by PI3K and ERK inhibitors, respectively.</div></div><div><h3>Conclusions</h3><div>Shear stress contributes to promoting <em>SOX2</em> and <em>BIRC5</em> expression by hPDLSCs, implicating the promotion of stemness and cell survival under serum starvation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106232"},"PeriodicalIF":2.2,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between agenesis of permanent teeth and other dental anomalies in nonsyndromic patients: Systematic review","authors":"Paula Cristiana Santos de Aguiar ,&nbsp;Caio Melo Mesquita ,&nbsp;Fabiana Evangelista Lerner ,&nbsp;Laura Bezerra Borges ,&nbsp;Rodrigo Rodrigues ,&nbsp;Walbert A. Vieira ,&nbsp;Luiz Renato Paranhos ,&nbsp;Rui Barbosa de Brito Júnior","doi":"10.1016/j.archoralbio.2025.106223","DOIUrl":"10.1016/j.archoralbio.2025.106223","url":null,"abstract":"<div><h3>Background</h3><div>Most cases of tooth agenesis are presented in the form of hypodontia with different patterns of missing teeth. In addition, there is a frequent clinical association between tooth agenesis and other anomalies.</div></div><div><h3>Objective</h3><div>To systematically analyze the literature on the prevalence of agenesis of permanent teeth associated with other dental anomalies in nonsyndromic patients.</div></div><div><h3>Material and methods</h3><div>An electronic search was performed in seven primary databases (Embase, LILACS, BBO, MedLine <em>via</em> PubMed, SciELO, Scopus, and Web of Science) and two additional ones (OATD, and ProQuest) to partially capture the \"gray literature.\" The PECO strategy was used to identify observational studies evaluating the prevalence of agenesis of permanent teeth associated with other dental anomalies in nonsyndromic patients, without restrictions on publication language or year. Two reviewers extracted the data and assessed the individual risk of bias of the eligible studies using the JBI Critical Appraisal Tools for use in JBI Systematic Reviews - Checklist for Cross-sectional, Case-Control, and Cohort Studies.</div></div><div><h3>Results</h3><div>The electronic search revealed 8659 records, seven of which met the eligibility criteria, and were included in the qualitative synthesis. The studies were published from 1961 to 2024. Patients with agenesis were more likely to present taurodontism, microdontia, enamel hypoplasia and distoangulation of the second premolar. Ectopic eruption, infraocclusion of the first deciduous molar and impacted tooth showed mixed results among the eligible studies.</div></div><div><h3>Conclusion</h3><div>Based on limited evidence, tooth agenesis was positively associated with the prevalence of other concomitant tooth agenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106223"},"PeriodicalIF":2.2,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143609877","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}