Archives of oral biology最新文献

{"title":"Comparison of dose and time effects of flavonoids, alone or in combination, on the induction of mineralization markers in human osteoblast-like cells","authors":"Rafaela Laruzo Rabelo ,&nbsp;Gabriel Pereira Nunes ,&nbsp;Geórgia Rondó Peres ,&nbsp;Rafael Araújo Rios ,&nbsp;Maria Eduarda de Souza ,&nbsp;Gabriela Pacheco de Almeida Braga ,&nbsp;Cristiane Duque","doi":"10.1016/j.archoralbio.2025.106285","DOIUrl":"10.1016/j.archoralbio.2025.106285","url":null,"abstract":"<div><h3>Objective</h3><div>This study compared the cytotoxicity, alkaline phosphatase (ALP) activity, and mineralization-inducing effects of flavonoids on human osteoblast-like Saos-2 cells.</div></div><div><h3>Design</h3><div>Saos-2 cells were treated with quercetin, myricetin, pinocembrin, kaempferol, isoquercitrin (Iso), rutin (Rut), taxifolin (Tax), ampelopsin (Amp), epigallocatechin-3-gallate (EGCG), and chrysin at 100, 50, and 25 µM for 48 h. Metabolic activity, ALP activity and mineral deposition were assessed via resazurin, thymolphthalein and Alizarin Red S staining assays, respectively, after 8 and 14 days. Calcium hydroxide was used as positive control, and untreated cells (DMEM) as negative control. Based on initial screening, Tax, Iso, Rut and Amp were selected for double combination treatments (at 50/50 µM and at 25/25 µM), and the same assays were performed. Data were analyzed using ANOVA and Tukey's test (α = 0.05).</div></div><div><h3>Results</h3><div>Comparing the flavonoids, regardless of time and concentration, only chrysin at 100 µM and 50 µM significantly reduced cell viability. Iso, Rut, Tax, and Amp exhibited the highest ALP activity and mineralized nodules formation, significantly outperforming the other flavonoids and DMEM control, particularly at 50 and 25 µM (p &lt; 0.05). Among the combinations, Tax+Iso, Tax+Amp and Tax+Rut, at 25/25 µM demonstrated higher ALP activity and enhanced mineral deposition compared to the other flavonoid combinations and DMEM (p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>Based on this preliminary in vitro model, Tax, Iso, Rut, and Amp, both individually and in combination, effectively promote biomineralization in Saos-2 cells, without inducing cytotoxic effects. These flavonoids hold significant potential for osteogenic applications, warranting further in vivo studies and clinical trials to optimize their therapeutic use.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106285"},"PeriodicalIF":2.2,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of omega-3 and omega-6 fatty acids on tumor suppressor pathways in mice tongue oral epithelial dysplasia","authors":"Julieta Don ,&nbsp;Victoria Ferrero ,&nbsp;Tamara Mazo ,&nbsp;Sol Bernárdez ,&nbsp;Nelso Barotto ,&nbsp;Mabel Brunotto ,&nbsp;María Eugenia Pasqualini","doi":"10.1016/j.archoralbio.2025.106283","DOIUrl":"10.1016/j.archoralbio.2025.106283","url":null,"abstract":"<div><h3>Objective</h3><div>This work aimed to evaluate the modulating effect of polyunsaturated fatty acids omega-3 and omega-6 on the expression of TP53 and specific primiRNAs 34 b/c in the development of premalignant characteristics in tongue epithelial dysplasias in-vivo experimental models</div></div><div><h3>Design</h3><div>BALB/c mice were divided into two diet groups: fish oil rich in omega-3 and corn oil rich in omega-6. Tongue dysplasia was generated by applying a 0.5 % solution of 7,12-dimethylbenzantracene (DMBA) in acetone. After sacrificing, the animals were assessed for omega-6 and omega-3, TP53/Ki67 immunostaining, Trp53R270H mutations, and primiRNA 34 b/c expression.</div></div><div><h3>Results</h3><div>Mice fed omega-6 showed higher dysplasias and Ki67 immunostaining percentages than those fed omega-3-rich oils. Furthermore, mice fed with corn oil rich in omega-6 showed significantly high average arachidonic-acid values, while the group fed cod oil rich in omega-3 showed substantially higher average values of Ecosapentanoic acid and Docosahexanoic acid in tongue tissue cell membranes. The primiRNA-34 b/c was upregulated in animals fed corn oil, enriched-omega-6 at 90 days.</div></div><div><h3>Conclusions</h3><div>Our findings suggest that dietary lipids influence the development of DMBA-induced premalignant features on the tongue of mice; therefore, a low intake of omega-6-rich oils and a higher intake of omega-3 could be recommended for preventing features similar to mucosal dysplasia, which could be an important preventive strategy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106283"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differences in oral microbiota between elderly and adults: A systematic review","authors":"Natalia S. Rozas ,&nbsp;Nicole Stephens ,&nbsp;Korina Yun-Fan Lu ,&nbsp;Cameron B. Jeter","doi":"10.1016/j.archoralbio.2025.106278","DOIUrl":"10.1016/j.archoralbio.2025.106278","url":null,"abstract":"<div><h3>Objective</h3><div>The aging process may significantly impact bacteria in the oral cavity (oral microbiome), leading to notable changes in its diversity and composition, potentially influencing systemic health. This systematic review aimed to compare the oral microbiota of elderly individuals (≥60 years) with that of younger adults.</div></div><div><h3>Design</h3><div>A comprehensive search of PubMed, Medline, EMBASE and CINAHL identified 794 studies published between 2005 and May 2023. Studies were included if the investigators compared the oral microbiome of elderly with younger adults using 16S rRNA sequencing. The JBI Critical Appraisal Checklist for Analytical Cross-Sectional Studies was employed to evaluate risk of bias. Ten full-text articles met the criteria for inclusion.</div></div><div><h3>Results</h3><div>The included studies revealed inconsistent results regarding alpha and beta diversity and the abundance of bacterial phyla with aging. Some studies reported increased diversity or abundance, others showed decreases, and several found no significant differences. However, this review identified a consistent decline in the abundance of <em>Haemophilus</em>, <em>Granulicatella</em>, and <em>Veillonella</em>, alongside an increase in <em>Comamonas</em>, in elderly populations across multiple studies. These genera may play critical roles in maintaining oral health and microbial stability in elderly populations, highlighting the need for further investigation.</div></div><div><h3>Conclusions</h3><div>The variability in microbial diversity and abudance suggests that confounding factors such as oral hygiene, systemic health, medication use, and lifestyle may complicate interpretation of results. In conclusion, future research should employ longitudinal designs with standardized methodologies and address confounding factors to better understand the aging oral microbiome and its implications for healthspan and quality of life.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106278"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143929030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transforming growth factor-β1 and its soluble receptor type 2 in saliva of young adults: Sex-related differences and predictive modeling of salivary concentrations","authors":"Marija S. Milić ,&nbsp;Bojan Dželetović ,&nbsp;Biljana Anđelski Radičević ,&nbsp;Nataša Milosavljević ,&nbsp;Nina Dimitrijević Jovanović ,&nbsp;Ivan Dožić ,&nbsp;Jelena Krunić ,&nbsp;Ljiljana Đukić","doi":"10.1016/j.archoralbio.2025.106279","DOIUrl":"10.1016/j.archoralbio.2025.106279","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate and compare salivary presence and levels of Transforming Growth Factor-β1 (TGF-β1) and its soluble receptor type 2 (TGFBR2), along with the biochemical profile of the unstimulated whole saliva (UWS) of healthy young males and females; and to assess the potential of the predictive modeling for estimating the active TGF-β1 and TGFBR2 levels based on sex and individual salivary biochemical profile.</div></div><div><h3>Design</h3><div>Study sample included 20 participants, both sexes, with the median value of age being 20.00 (1.00) years. Total and active TGF-β1 and TGFBR2 levels were tested with ELISA. Biochemical analysis of saliva, including lactat dehydrogenase (LDH) activity and uric acid levels, was conducted via spectrophotometry. Salivary pH and buffer capacity were determined with potentiometry. To model the relationship of active TGF-β1 and TGFBR2 levels with sex and individual salivary biochemical profiles, multivariate regression analysis was employed.</div></div><div><h3>Results</h3><div>The median/mean values of active TGF-β1 (33.13 (27.26) vs. 14.24 (9.89) pg/ml, p = 0.013), uric acid (260.00 (136.00) vs. 199.00 (74.50) μmol/L, p = 0.031), and LDH activity (82.00 ± 45.23 vs. 42.40 ± 30.99 U/L, p = 0.035) were significantly higher in males vs. females, respectively. Multivariate regression method demonstrated 71.9 % accuracy in predicting the levels of active TGF-β1, while for TGFBR2 the accuracy was 86.01 %.</div></div><div><h3>Conclusions</h3><div>Salivary levels of active TGF-β1 are significantly higher in young healthy males compared to females. The multivariate regression model demonstrates promising predictive potential for estimating the levels of active TGF-β1 and TGFBR2 in young healthy individuals, based on sex and individual salivary biochemical profiles.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106279"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical expression of p53/TP53 in histologically negative mucosal margins of head and neck squamous cell carcinomas and usefulness in predicting locoregional recurrence: A systematic review and meta-analysis","authors":"Gopikrishnan Vijayakumar ,&nbsp;Sithara Aravind ,&nbsp;Anand Vijaya Narayanan ,&nbsp;Mala Kamboj ,&nbsp;Sangeeta K. Nayanar ,&nbsp;Adarsh Kumar ,&nbsp;Anjali Narwal","doi":"10.1016/j.archoralbio.2025.106281","DOIUrl":"10.1016/j.archoralbio.2025.106281","url":null,"abstract":"<div><h3>Objective</h3><div>To compare the expression of p53/TP53 in histologically negative mucosal margins of head and neck squamous cell carcinoma with locoregional recurrence.</div></div><div><h3>Design</h3><div>A complete search through MEDLINE by PubMed, Google Scholar, Scopus and EMBASE until 31st January 2023, without period restriction. Articles on head and neck squamous cell carcinoma and p53/TP53 expression were included. Sensitivity, specificity, positive and negative predictive values, positive and negative likelihood ratio, Odds and relative risk ratio of p53/TP53 in histologically negative surgical margins and its effectiveness in predicting recurrences were derived and subjected to meta-analysis using Open Meta analyst for Sierra (10.12) software.</div></div><div><h3>Results</h3><div>The specificity of p53 immunohistochemistry in predicting locoregional recurrence was 0.844 (95 % CI:0.78 ± 0.89) (p-value- &lt; 0.001) with a negative likelihood ratio of 0.487 (95 % CI: 0.35 ± 0.67) (p-value &lt; 0.001) and positive likelihood ratio of 3.032 (95 % CI: 2.17 ± 4.22) (p-value &lt; 0.001). The relative risk of locoregional recurrence in the histologically negative surgical margin with p53 positivity was 3.13 with statistical significance (Odds ratio: 5.249, CI:3.176 ± 8.676, p-value &lt; 0.001). Subgroup meta-analysis based on the location of head and neck SCC shows significant prediction for recurrence in p53 positive margins with more significant results in laryngeal carcinoma recurrence (p value &lt; 0.001) (overall p value &lt; 0.000).</div></div><div><h3>Conclusions</h3><div>Immunohistochemical expression of p53 in histologically negative surgical margins of head and neck squamous cell carcinoma is a significant predictor of locoregional recurrence and prognosis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106281"},"PeriodicalIF":2.2,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From scent to saliva: The saliva-stimulating effect of terpenes in dry mouth","authors":"Wiktoria Potocka ,&nbsp;Zainab Assy ,&nbsp;Annina P. van Splunter ,&nbsp;Marja L. Laine ,&nbsp;Floris J. Bikker","doi":"10.1016/j.archoralbio.2025.106282","DOIUrl":"10.1016/j.archoralbio.2025.106282","url":null,"abstract":"<div><h3>Objectives</h3><div>There is an increasing trend towards the use of bioactive compounds in oral healthcare. Some have been identified as sialagogues, such as mastic resin and its main isolate, α-pinene. These act as acetylcholinesterase inhibitors (AChEIs), consequently increasing salivary flow. With up to half of the global population experiencing dry mouth symptoms, there is a clear need for new interventions.</div></div><div><h3>Design</h3><div>An <em>in vitro</em> screening of a 148-compound library was conducted to identify novel AChEIs. Following this, four compounds were chosen for an <em>in vivo</em> validation and were administered through nasal spray pumps to act as an olfactory stimulus in healthy individuals (n = 12) to evaluate their sialagogic effects. Saliva quantity, rheology, and composition (total protein concentration and MUC5B concentration) were analysed before and after the use of the compounds.</div></div><div><h3>Results</h3><div>This study confirmed α-pinene, and identified basil, eugenol and guaiacol as AChEIs <em>in vitro</em>. Basil and guaiacol significantly increased salivary flow, while simultaneously basil preserved the spinnbarkeit levels. α-Pinene, basil and guaiacol increased the subjective feeling of moistness of the mouth. Total salivary protein concentration was not affected by scent of these compounds, however, α-pinene, basil and guaiacol significantly increased MUC5B output. Furthermore, basil was found to have the most pleasant scent out of the four compounds.</div></div><div><h3>Conclusions</h3><div>In conclusion, this study showed the potency of basil and guaiacol as novel sialagogues. However, further research is necessary to assess the long-term effects and efficacy in patients diagnosed with dry mouth.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106282"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143921734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “m6A-related genes ALKBH5 and RBMX as prognostic and progression biomarkers in Chinese oral squamous cell carcinoma patients” [Archives of Oral Biology 170 (2024) 106149]","authors":"Yong Liu ,&nbsp;Jiaying Nie ,&nbsp;Ying Huang ,&nbsp;Yunyan Yang ,&nbsp;Wenen Su ,&nbsp;Yumei Zhang ,&nbsp;Zhuoqiao Gao ,&nbsp;Shaohui Deng ,&nbsp;Meilin Li ,&nbsp;Shaoyan Lian ,&nbsp;Jieying Li ,&nbsp;Chaoqun Liu","doi":"10.1016/j.archoralbio.2025.106230","DOIUrl":"10.1016/j.archoralbio.2025.106230","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106230"},"PeriodicalIF":2.2,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-205-3p inhibits porphyromonas gingivalis lipopolysaccharide-induced human umbilical vein endothelial cells inflammation and apoptosis by targeting PRMT5","authors":"Jinsheng Wu ,&nbsp;Weiyi Li ,&nbsp;Ying Tang ,&nbsp;Chang Wu ,&nbsp;Weishan Li","doi":"10.1016/j.archoralbio.2025.106276","DOIUrl":"10.1016/j.archoralbio.2025.106276","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the regulatory mechanism of miR-205–3p in <em>Porphyromonas gingivalis</em> lipopolysaccharide (P.g-LPS)-induced atherosclerosis.</div></div><div><h3>Design</h3><div>In an in vitro setting, human umbilical vein endothelial cells (HUVECs) were exposed to P.g-LPS to simulate the vascular endothelial damage induced by periodontitis. Subsequently, ELISA and flow cytometry were employed to assess the inflammatory response and apoptotic status of these cells.To quantify the expression levels of protein arginine methyltransferase 5 (PRMT5), BCL2-associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), P65 and miR-205–3p within the HUVECs, Western Blot and qPCR were respectively utilized. Moreover, small interfering RNA (siRNA) targeting PRMT5 and miR-205–3p were applied to monitor the changes in PRMT5 expression. Bioinformatics analysis was carried out to predict the potential binding sites between miR-205–3p and PRMT5. Finally, the interaction between miR-205–3p and PRMT5 was validated through the dual-luciferase reporter assay.</div></div><div><h3>Results</h3><div>The results indicate that P.g-LPS intervention exacerbates damage to HUVECs and increases the expression of PRMT5. Silencing PRMT5 reduces cell inflammation and apoptosis. After stimulation with P.g-LPS, the level of miR-205–3p decreases, and its overexpression alleviates inflammation and apoptosis in the cells. Bioinformatics analysis and dual luciferase reporter assays confirm that PRMT5 is a target of miR-205–3p, and the overexpression of PRMT5 can reverse the protective effects of miR-205–3p.</div></div><div><h3>Conclusion</h3><div>miR-205–3p can mitigate vascular endothelial injury by decreasing PRMT5 expression, providing new insights for potential treatments.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106276"},"PeriodicalIF":2.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between pica practice, oral health, and cariogenic microorganisms of US pregnant women: A cross-sectional study","authors":"Yan Wu ,&nbsp;Jin Xiao ,&nbsp;Noha Rashwan ,&nbsp;Abigail Morrissey ,&nbsp;Dina Yousif ,&nbsp;Nisreen Al Jallad ,&nbsp;Kevin Fiscella ,&nbsp;TongTong Wu ,&nbsp;Brenda A.Z. Abu","doi":"10.1016/j.archoralbio.2025.106277","DOIUrl":"10.1016/j.archoralbio.2025.106277","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effects of pica practices during pregnancy on oral health risks and outcomes.</div></div><div><h3>Design</h3><div>A cross-sectional study was conducted among pregnant women in New York. Demographic, medical history and oral hygiene practice data were collected using validated questionnaires. Comprehensive oral examinations included caries and periodontal status (plaque index and bleeding on probing). Clinical samples (saliva, plaque, and pica substances obtained from participants) were processed to assess the carriage of cariogenic microorganisms (<em>Streptococcus mutans</em> and <em>Candida</em> species) using culturing-dependent and -independent methods. We statistically compared oral clinical and microbial outcomes of pica and non-pica participants.</div></div><div><h3>Results</h3><div>Among 20 women enrolled (8 (40 %) with pica and 12 (60 %) without pica), the mean gestational age was 25.55 ± 8.82 weeks. <em>Candida</em> species were detected in 25 % of pica samples (n = 2), predominantly from ice samples. The pica group exhibited poorer oral health conditions, showing more decayed-missing-filled teeth and surfaces than the non-pica counterparts (<em>p</em> = 0.016). Although not statistically significant, salivary carriage of <em>C. albicans</em> was 2.8 times higher in women with pica compared to those without (3.3 ± 5.7 × 10³ CFU/ml versus 1.2 ± 3.8 × 10³ CFU/ml in non-pica, <em>p</em> = 0.093). Women with pica reported markedly higher prevalence of anemia (50 %) compared to non-pica participants (8.3 %) (<em>p</em> = 0.035).</div></div><div><h3>Conclusions</h3><div>Pica practices were associated with maternal oral health risks, imploring that clinicians manage pica behaviors to mitigate oral diseases during pregnancy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106277"},"PeriodicalIF":2.2,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palatal morphology: A systematic review of the association of palatal shape with genetic ancestry, sex and age","authors":"Thao Liang Chiam ,&nbsp;Harry Perkins ,&nbsp;Toby Hughes ,&nbsp;Lyle Palmer ,&nbsp;Denice Higgins","doi":"10.1016/j.archoralbio.2025.106275","DOIUrl":"10.1016/j.archoralbio.2025.106275","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to review the literature regarding palatal shape variation, and its influence on estimating genetic ancestry, sex, and age.</div></div><div><h3>Design</h3><div>A literature search was performed using PubMed, Scopus and Embase databases, from inception up to January 2025. Original research articles in English examining variation in shape of human hard palate were included, irrespective of study type or publication year. Articles regarding craniofacial anomalies, pathologies and orthodontic treatment were excluded. The risk of bias was assessed independently by two reviewers using the Joanna Briggs Institute critical appraisal tool.</div></div><div><h3>Results</h3><div>Twenty-two studies fulfilled the selection criteria. Dental casts (<em>n</em> = 13) and crania (<em>n</em> = 8) were predominantly utilised in the studies. Geometric morphometrics emerged as the predominant method for palatal shape analysis, followed by fourth-order polynomial curve fitting, visual assessments and ratios. Five studies examined ancestral variation in palatal shape, with four reporting differences between population groups. These studies, however, utilised fixed landmarks only, offering limited insight. Sexual dimorphism of palatal shape was extensively investigated (<em>n</em> = 12), with large numbers of semi-landmarks employed in two studies, but association was reported in four studies only. Palatal shape was reported to change from birth to adulthood but remained unchanged after adulthood.</div></div><div><h3>Conclusion</h3><div>This review highlights that palatal shape may aid ancestry classification but remains inconsistent to classify sex. Palatal shape changes during growth are allometric and hence need to be considered in forensic investigations. More standardised and robust methods may better evaluate the effects of ancestry, sex and age on 3D palatal variation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"175 ","pages":"Article 106275"},"PeriodicalIF":2.2,"publicationDate":"2025-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143888178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}