Archives of oral biology最新文献

{"title":"Survival of periodontal ligament myofibroblasts after short-term mechanical strain in rats and in vitro: Could myofibroblasts contribute to orthodontic relapse?","authors":"Raquel Veloso Varanda ,&nbsp;Jyoti Kumari ,&nbsp;René E.M. van Rheden ,&nbsp;Vincent M.J.I. Cuijpers ,&nbsp;Marjon Bloemen ,&nbsp;Fleur Göllesch ,&nbsp;Johannes W. Von den Hoff ,&nbsp;Sjoerd Henneman ,&nbsp;Rui Xie ,&nbsp;Frank A.D.T.G. Wagener ,&nbsp;C. Maarten Suttorp","doi":"10.1016/j.archoralbio.2025.106173","DOIUrl":"10.1016/j.archoralbio.2025.106173","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate <em>in vivo</em> whether myofibroblasts formed in the PDL after exposure to short-term high experimental orthodontic forces in rats survive. To study <em>in vitro</em> whether human PDL fibroblasts can differentiate into myofibroblasts and survive when chemical or mechanical stimuli are removed.</div></div><div><h3>Design</h3><div>Nine 6-week-old male Wistar rats were used in this experiment. Rat molars were exposed to high but rapidly decreasing experimental orthodontic forces by applying a rubber band and analyzed for the presence of myofibroblasts using ASMA staining. <em>In vitro</em>, human periodontal ligament (PDL) fibroblasts were exposed to transforming growth factor β1 (TGFβ1) and/or mechanical stress and monitored for myofibroblast formation and survival after these stimuli were abrogated.</div></div><div><h3>Results</h3><div><em>In vivo</em> exposure to orthodontic forces strongly induced myofibroblast formation in the stretched regions of the PDL. Furthermore, many PDL myofibroblasts remained present 6 days after exposure to these short-term high orthodontic forces. Human PDL fibroblasts were shown to differentiate into myofibroblasts after 2 days of TGFβ1 exposure and survive for at least 2 more days after removing chemical stimuli (TGFβ1) or mechanical strain. Under in vitro conditions, both TGFβ1 and mechanical strain for 3 days promoted (myo)fibroblast formation, and these cells persisted for 3 more days after the removal of both stimuli.</div></div><div><h3>Conclusions</h3><div>PDL myofibroblasts survive after the removal of mechanical strain <em>in vivo</em> and <em>in vitro</em>. This supports the hypothesis that myofibroblasts, which form in response to mechanical strain and chemical cues in the periodontal ligament (PDL), play a role in relapse following orthodontic tooth movement.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106173"},"PeriodicalIF":2.2,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142960250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of mandibular overdentures on maxillary resorption and its association with masticatory function: A prospective longitudinal study with 3 years of follow-up","authors":"Fernanda Faot ,&nbsp;Samille Biasi Miranda ,&nbsp;Laura Lourenço Morel ,&nbsp;Anna Paula Da Rosa Possebon ,&nbsp;Alessandra Juliê Schuster ,&nbsp;Luciana de Rezende Pinto ,&nbsp;Altair Antoninha Del Bel Cury","doi":"10.1016/j.archoralbio.2024.106171","DOIUrl":"10.1016/j.archoralbio.2024.106171","url":null,"abstract":"<div><h3>Objective</h3><div>This longitudinal clinical study monitored annually the maxillary and mandibular bone remodeling and masticatory function in complete denture (CD) wearers rehabilitated with implant-retained mandibular overdentures (MO) over three years and combined radiographic and masticatory function data to assess the correlation between bone remodeling and masticatory function.</div></div><div><h3>Design</h3><div>Thirty-nine MO wearers were monitored annually to assess changes in: i) residual ridge in the anterior and posterior maxillary region; ii) posterior height and posterior area index (PAI) in the mandible; and iii) masticatory function. Bone remodeling was measured through linear and angular measurements using panoramic radiographs. Masticatory function was evaluated by swallowing threshold test, to determine the median particle size (X50) and homogenization index (B). A mixed-effects multilevel regression model estimated differences between radiological and masticatory parameters over time, with associations verified using Pearson's correlation test.</div></div><div><h3>Results</h3><div>Significant changes in mandibular bone variables (PAI and L1/R1, L2/R2, L3/R3; p ≤ 0.01) occurred when transitioning from CD to MO, showing a reduction in PAI values and slight reduction in mandibular linear measurements. Improvements in masticatory parameters were also observed, highlighting the benefits of transitioning to MO. Over three years of MO usage, significant changes in all radiographic bone parameters were found, with increased PAI and maxillary bone values in the first year. The correlations between masticatory function and radiological parameters indicated that mandibular morphology can significantly influence masticatory function especially in patients wearing CDs. Following the transition to MO, only one consistent significant correlation was observed between X50 and R3/L3 at the 1-year and 3-year intervals.</div></div><div><h3>Conclusion</h3><div>Continuous changes in bone parameters were observed in MO wearers, with stabilization of maxillary ridge resorption and ongoing mandibular remodeling, alongside slight changes in masticatory function, suggesting transitory correlations between linear changes in the posterior region of mandible and masticatory function over time.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106171"},"PeriodicalIF":2.2,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143026050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of ionizing radiation on osteoblastic cells: In vitro insights into the etiopathogenesis of osteoradionecrosis","authors":"Bruna Bastos Silveira ,&nbsp;Larissa Di Carvalho Melo ,&nbsp;Mylene Martins Monteiro ,&nbsp;Juliana Amorim dos Santos ,&nbsp;Paula Elaine Diniz dos Reis ,&nbsp;Angelica Amorim Amato ,&nbsp;Taia Maria Berto Rezende ,&nbsp;Eliete Neves Silva Guerra","doi":"10.1016/j.archoralbio.2024.106172","DOIUrl":"10.1016/j.archoralbio.2024.106172","url":null,"abstract":"<div><h3>Objective</h3><div>This <em>in vitro</em> study aimed to analyze the effects of ionizing radiation on immortalized human osteoblast-like cells (SaOS-2) and further assess their cellular response in co-culture with fibroblasts. These analyses, conducted in both monoculture and co-culture, are based on two theoretical models of osteoradionecrosis – the theory of hypoxia and cellular necrosis and the theory of the radiation-induced fibroatrophic process.</div></div><div><h3>Design</h3><div>SaOS-2 cells were exposed to ionizing radiation and evaluated for cell viability, nitric oxide (NO) production, cellular morphology, wound healing, and gene expression related to the PI3K-AKT-mTOR pathway. SaOS-2 cells were co-cultured with human gingival fibroblasts using transwell membranes and subjected to the same irradiation. Subsequent evaluations included cell viability, NO levels, and gene expression analysis.</div></div><div><h3>Results</h3><div>After 24 hours, a 16 Grays dose reduced cell viability by 40 % (p &lt; 0.0001) and increased NO production by 14 % (p &lt; 0.05). Additionally, the nuclear area was enlarged by 18 % (p &lt; 0.01), and the nucleus-to-cytoplasm ratio in non-stimulated cells was around 33 %, but after radiation, this ratio increased to nearly 100 %. Also, there was a delay in wound closure of 6.6 % (p &lt; 0.0001) post-irradiation and a trend toward down-regulation of genes related to the PI3K–AKT–mTOR pathway (p &gt; 0.05). Under co-culture conditions, the dose of 16 Grays did not affect cell viability but increased NO production by 14 % (p &lt; 0.001) and tended to up-regulate markers of the PI3K–AKT–mTOR pathway (p &gt; 0.05).</div></div><div><h3>Conclusions</h3><div>The findings of this study demonstrate that an irradiation dose of 16 Grays induces a reduction in cell viability, an increase in NO production, and various other metabolic and morphologic effects on osteoblastic cells while emphasizing the impact of intercellular interaction in the etiopathogenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106172"},"PeriodicalIF":2.2,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143018155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of craniofacial tissue aging in genetically diverse HET3 male mice with longevity treatment of 17-alpha estradiol","authors":"Rami Alsabbagh ,&nbsp;Leah LaVerde ,&nbsp;Emma Chufar ,&nbsp;Jake W. Willows ,&nbsp;Kristy L. Townsend ,&nbsp;Sarah B. Peters","doi":"10.1016/j.archoralbio.2024.106170","DOIUrl":"10.1016/j.archoralbio.2024.106170","url":null,"abstract":"<div><h3>Objective</h3><div>The objective of our study was to 1) characterize craniofacial tissue aging in the new, genetically diverse HET3 mouse model; and 2) ascertain whether increased longevity with 17-alpha estradiol (17αE2) treatment in male mice also improved the health of these tissues. The HET3 mice are a four-strain cross preferred and recommended by the National Institute of Aging to identify longevity treatments and test their ability to reduce age-related pathologies. Previous reports demonstrated increased longevity in male, but not female, HET3 mice with 17αE2 administration.</div></div><div><h3>Design</h3><div>Male mice were raised to approximately 8 months (young), 16 months (middle-aged), and 25 months (old). Middle-aged and old mice were administered a diet supplemented with 17αE2 for 19 weeks. We quantified craniofacial tissue volume and density changes with micro-computed tomography followed by histology.</div></div><div><h3>Results</h3><div>Micro-CT showed that the alveolar bone volume and density did not change with age or treatment. Enamel volume and density changed with age but not treatment. Histology revealed region-specific degeneration of periodontal ligaments (PDLs) with age. Cellular cementum demonstrated age-related density decreases but no change in volume. However, cementum volume and density increased with 17αE2 treatment. Dentin volume increased with age whereas density decreased with age, which were attenuated by 17αE2 treatment.</div></div><div><h3>Conclusions</h3><div>The HET3 mice present an excellent model with which to study the heterogeneous nature of tooth aging and the effects of longevity interventions. We provide novel data on how 17αE2 improves healthspan by modifying age-related changes in the molar dentin and cementum of male mice.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106170"},"PeriodicalIF":2.2,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the influence of sports-induced trauma on temporomandibular disorders: A systematic review and meta-analysis","authors":"Ravinder S. Saini ,&nbsp;Abdulmajeed Okshah ,&nbsp;Mohammed Haneef ,&nbsp;Syed Altafuddin Quadri ,&nbsp;Mudita Chaturvedi ,&nbsp;Seyed Ali Mosaddad ,&nbsp;Artak Heboyan","doi":"10.1016/j.archoralbio.2024.106168","DOIUrl":"10.1016/j.archoralbio.2024.106168","url":null,"abstract":"<div><h3>Objective</h3><div>The main aim of this study was to identify the existing literature on the association between sporting activities and temporomandibular disorders and to critically appraise evidence of this association through a systematic review and meta-analysis.</div></div><div><h3>Design</h3><div>A comprehensive search was conducted using PubMed, ScienceDirect, Dimensions, Google Scholar, Cochrane Library, and the Education Resources Information Centre (ERIC). Articles were selected using pre-specified eligibility criteria. In addition, the quality of eligible studies was assessed using the National Institutes of Health (NIH) for observational cohort and cross-sectional studies. Data were systematically extracted and analyzed.</div></div><div><h3>Results</h3><div>Temporomandibular disorder symptoms, such as myofascial pain, clicking sounds, and musculoskeletal symptoms, were comparably prevalent among athletes and non-athletes, with some studies reporting contrasting results. However, meta-analyses of the comparisons demonstrated statistically insignificant differences between athletes and non-athletes. Additionally, there was a significant reduction in temporomandibular disorder symptoms when mouthguards were used.</div></div><div><h3>Conclusions</h3><div>This comprehensive analysis of existing evidence highlights the various symptoms and clinical manifestations of TMD among athletes, including myofascial pain, clicking sounds, and musculoskeletal symptoms. This study established different prevalence rates in different sports.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106168"},"PeriodicalIF":2.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143154691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between oral microbiome dysbiosis and Sjogren Syndrome. A systematic review of clinical studies","authors":"Ravleen Nagi ,&nbsp;Sanjana Santhosh Kumar ,&nbsp;Megha Sheth ,&nbsp;Ashwini Deshpande ,&nbsp;Junad Khan","doi":"10.1016/j.archoralbio.2024.106167","DOIUrl":"10.1016/j.archoralbio.2024.106167","url":null,"abstract":"<div><h3>Objectives</h3><div>This systematic review investigates the association of oral microbiome dysbiosis with Sjogren Syndrome (SS).</div></div><div><h3>Materials and methods</h3><div>Indexed databases (PubMed/Medline, EMBASE, OVID, Web of Science, and Scopus) were independently searched for relevant manuscripts published until August 2024. Clinical studies on oral microbial flora count and diversity in SS patients were included. Risk of bias across individual studies was performed using the Risk of Bias in Nonrandomized Studies of Interventions tool.</div></div><div><h3>Results</h3><div>Out of the initial 295 studies, 15 clinical studies met the selection criteria. The protocols were similar across the studies but varied in diagnostic criteria for SS, salivary flow estimation methods, dental and periodontal status findings, and the type of oral microbes observed. Out of 15 studies, 14 showed an alteration in the oral microbiome and differences in microbial diversity in SS patients. Higher oral microbial counts of Prevotella, Viellonella, and Firmicutes in SS were reported, whereas a higher prevalence of caries-associated bacteria Streptococcus, Lactobacillus, and Viellonella was found in SS patients. Overall, the studies had a low risk of bias.</div></div><div><h3>Conclusions</h3><div>The findings of the present review have shown the existence of significant oral microbial dysbiosis and differences in microbial diversity in SS patients compared to healthy subjects. Future well-designed longitudinal studies are needed to validate the results.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106167"},"PeriodicalIF":2.2,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142973803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of bidirectional causal association between temporomandibular disorders and five mental disorders","authors":"Yifan Wu ,&nbsp;Zhenguo Shen ,&nbsp;Bohan Chen ,&nbsp;Shuyan Sheng ,&nbsp;Zhenghan Zhang ,&nbsp;Wei Shao ,&nbsp;Tian Xing","doi":"10.1016/j.archoralbio.2024.106169","DOIUrl":"10.1016/j.archoralbio.2024.106169","url":null,"abstract":"<div><h3>Objective</h3><div>This study utilized two-sample Mendelian randomization (TSMR) to investigate the bidirectional causal associations between temporomandibular disorders (TMD) and five mental disorders.</div></div><div><h3>Methods</h3><div>Single-nucleotide polymorphisms (SNPs) linked to TMD were extracted from the Genome-Wide Association Studies (GWAS) database. The SNPs selected as instrumental variables (IVs) were required to have strong associations with the exposure phenotype and to meet the assumptions of Mendelian randomization (MR) analysis. The primary method employed for TSMR analysis was the Inverse Variance-Weighted (IVW) method. Additionally, the Weighted Median, Weighted Mode, and MR Egger methods were used as secondary approaches to ensure the reliability and credibility of the results. Furthermore, heterogeneity, pleiotropy, and sensitivity tests were conducted to ensure the accuracy and robustness of the findings. After MR analysis, we performed reverse analysis to explore the bidirectional association between TMD and five mental disorders.</div></div><div><h3>Results</h3><div>Our study provided evidence of a positive correlation between TMD and both anxiety (P = 5.94 × 10⁻¹⁵, OR = 1.5253, 95 % CI = 1.3719–1.6959) and depression (P = 3.79 × 10⁻¹², OR = 1.4243, 95 % CI = 1.2890–1.5738). In the reverse TSMR analysis, a positive association was found between anxiety and TMD (P = 0.0126, OR = 1.0664, 95 % CI = 1.0139–1.1217). However, no causal relationship was found between TMD and depression, insomnia, bipolar disorder, or schizophrenia (P &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>These evidences indicated a bidirectional relationship between TMD and anxiety, and TMD may also contribute to the development of depression.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106169"},"PeriodicalIF":2.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinicopathological comparison and cytokeratin-10 expression between Lichen planus and oral lichenoid lesions","authors":"Milena Duarte Moreira,&nbsp;Luanna Canal Pereira,&nbsp;Tahyna Duda Deps,&nbsp;Tânia Regina Grão Velloso,&nbsp;Letícia Nogueira da Gama-de-Souza,&nbsp;Liliana Aparecida Pimenta-Barros,&nbsp;Danielle Resende Camisasca","doi":"10.1016/j.archoralbio.2024.106166","DOIUrl":"10.1016/j.archoralbio.2024.106166","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to compare clinicopathological features and immunostaining for cytokeratin-10 between oral lichen planus and oral lichenoid lesions.</div></div><div><h3>Design</h3><div>This was a retrospective longitudinal study comparing lichen planus and oral lichenoid lesions diagnosed at the Oral Pathological Anatomy Service that analyzed sociodemographic and clinicopathological data and CK10 expression. Chi-square tests, Fisher's exact tests and Mann-Whitney tests or Student's t tests were used when appropriate, and p values &lt; 0.05 were considered significant.</div></div><div><h3>Results</h3><div>A total of 23 lichen planus and 23 lichenoid lesions were included. There was an association between oral lichen planus and symptomatology (p = 0.031). The buccal mucosa was the most affected site in both groups: 20 patients (87.0 %) showed oral lichen planus, and 16 patients (69.6 %) oral lichenoid lesions. Bilateral (p &lt; 0.001) striae (p = 0.004) are more characteristic of oral lichen planus. Oral lichen planus was associated with degeneration of the basal layer (p = 0.049) and with mild epithelial dysplasia (p &lt; 0.001). Cytokeratin-10 immunostaining was similar between the groups.</div></div><div><h3>Conclusions</h3><div>A continuous follow-up is necessary to identify different patterns of malignant transformation between groups of lesions, as well as for comparisons with lesions with a higher malignant transformation rate.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106166"},"PeriodicalIF":2.2,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142916504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative proteomics in rat saliva stimulated with pilocarpine and isoprenaline","authors":"Marcin Pasternak ,&nbsp;Weronika Pogoda ,&nbsp;Piotr Ceranowicz ,&nbsp;Jakub Cieszkowski ,&nbsp;Józef Madej ,&nbsp;Rafał Olszanecki ,&nbsp;Maciej Suski","doi":"10.1016/j.archoralbio.2024.106165","DOIUrl":"10.1016/j.archoralbio.2024.106165","url":null,"abstract":"<div><h3>Objective</h3><div>Saliva is increasingly being recognized as a convenient and informative reservoir of proteins that could serve as indicators of various diseases. As the literature remains taciturn with regard to saliva collection methods in rodents, our objective was to provide the protocol for a comprehensive quantitative proteomic assessment of stimulated rat saliva.</div></div><div><h3>Design</h3><div>We applied the next-generation proteomic methodology (directDIA) to compare qualitatively and quantitatively stimulated rat saliva specimens obtained from pilocarpine alone and pilocarpine in combination with isoprenaline.</div></div><div><h3>Results</h3><div>Collectively, we identified 581 protein groups with high confidence across all samples included in the analysis, with the dynamic range of the identifications estimated to cover 5 orders of magnitude difference between the most abundant and least abundant salivary proteins. Our data evidenced that pilocarpine-stimulated saliva collection showed a trend towards more protein groups identified; however, quantitative reproducibility was preferable after dual stimulation.</div></div><div><h3>Conclusions</h3><div>The main advantage of the double stimulation strategy is the quantitative stability of the salivary proteome, crucial for quantitative salivaomic experiments. We postulate that the latter in combination with the depth of proteome analysis provided by the directDIA technique constitutes a novel analytical tool in research studies designed to unravel the saliva protein composition and its changes in vivo.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106165"},"PeriodicalIF":2.2,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142901093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The periodicity of enamel laminations in human deciduous molars","authors":"Petrina Barnard ,&nbsp;Gina McFarlane ,&nbsp;Chris Deter ,&nbsp;Carolina Loch ,&nbsp;Patrick Mahoney","doi":"10.1016/j.archoralbio.2024.106164","DOIUrl":"10.1016/j.archoralbio.2024.106164","url":null,"abstract":"<div><h3>Objective</h3><div>Enamel laminations are closely spaced incremental lines that run parallel to Retzius lines or the developing enamel surface. Here, the timing of enamel laminations is calculated for naturally exfoliated deciduous molars (n = 111) from three modern-day populations (Aotearoa New Zealand, Britain and Canada).</div></div><div><h3>Design</h3><div>Teeth were sectioned using standard histological methods and examined using a high-powered microscope. Mean daily secretion rates (DSR) were calculated for the outer enamel of each molar in cuspal, lateral and cervical enamel regions. These DSRs were used to determine the periodicity of enamel growth across laminations in each region. Lamination periodicity was compared between populations and sexes, and within molars to assess the relationship between lamination periodicity and the angle between laminations and the outer surface.</div></div><div><h3>Results</h3><div>Laminations were present in 57 % of all molars (<em>n</em> = 63 out of <em>n</em> = 111). Their presence did not vary between populations or by sex. A mean two-day periodicity was observed in cuspal and lateral outer enamel sampling regions. A mean one-day periodicity was observed in the cervical outer enamel. The angle of laminations relative to the outermost surface of the enamel was significantly related to the presence of laminations.</div></div><div><h3>Conclusions</h3><div>A two-day periodicity for laminations indicates that this incremental marking is not a reliable proxy for a circadian 24-hour rhythm in human deciduous molars. The orientation of laminations was similar to Retzius lines but differed to the orientation of cross-striations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106164"},"PeriodicalIF":2.2,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142883859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}