Archives of oral biology最新文献

{"title":"Different densities of Langerhans cells among oral squamous cell carcinoma, lip squamous cell carcinoma and oral potentially malignant disorders: Systematic review and network meta-analysis","authors":"Naiara Alves Marega ,&nbsp;Luana Paula Borges da Costa e Silva ,&nbsp;Analú Barros de Oliveira ,&nbsp;Rafaella Luiza Bergamaschi de Carli ,&nbsp;Andreia Bufalino ,&nbsp;Elaine Maria Sgavioli Massucato ,&nbsp;Túlio Morandin Ferrisse","doi":"10.1016/j.archoralbio.2025.106372","DOIUrl":"10.1016/j.archoralbio.2025.106372","url":null,"abstract":"<div><h3>Objective</h3><div>to evaluate whether Langerhans cells (LC) (CD1a+, CD207 +) show quantification differences in oral squamous cell carcinoma (OSCC), lip squamous cell carcinoma (LSCC), and oral potentially malignant disorders (OPMDs), due to previously conflicting results.</div></div><div><h3>Design</h3><div>PubMed, Scopus, Embase, Lilacs, and Web of Science were the databases searched. The protocol was registered in the Open Science Framework (OSF). Rayyan software was used for duplicate removal. Risk of bias was assessed using the Joanna Briggs Institute (JBI) scale for cross-sectional studies. Meta-analyses were conducted using R software (v3.6.3) with the \"NETMETA\" package. Quality of evidence was evaluated using the CINeMA tool. In total, 26 articles were included for qualitative and 14 articles for quantitative analysis</div></div><div><h3>Results</h3><div>Higher CD1a+ and CD207 + LC levels were observed in low-grade OSCC compared to well and moderately differentiated OSCC. In addition, reductions of CD1a+ and CD207 + LC were associated with locoregional metastasis to lymph nodes. Among OPMDs, oral lichen planus (OLP) showed the highest CD1a+ and CD207 + density. Significant reductions in LC counts were noted in oral leukoplakia (OL), oral submucous fibrosis (OSF), and OSCC. In LSCC, CD1a+ LCs were significantly reduced compared to actinic cheilitis (AC). Sampling and statistical biases were identified in 7 and 17 studies respectively.</div></div><div><h3>Conclusion</h3><div>Differences in CD1a+ and CD207 + LC densities among OSCC, LSCC, and OPMDs may cooperate in understanding of their immunopathogenesis and support diagnostic and therapeutic strategies in these conditions. Furthermore, CD1a+ and CD207 + density reduction may have the potential to be used as a biomarker for oral malignant transformation. In general, a high confidence rate was observed for CD207 + LC and a moderate confidence for CD1a+ LC was noted.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106372"},"PeriodicalIF":2.1,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of IL1β, IL6, and IL10 genetic variants in susceptibility to recurrent aphthous stomatitis: A systematic review and bioinformatics approach","authors":"Meircurius Dwi Condro Surboyo ,&nbsp;Lalu Muhammad Irham ,&nbsp;Pamela Handy Cecillia ,&nbsp;Benni Iskandar ,&nbsp;Permata Suka Admanegara ,&nbsp;Arvind Babu Rajendra Santosh ,&nbsp;Diah Savitri Ernawati","doi":"10.1016/j.archoralbio.2025.106373","DOIUrl":"10.1016/j.archoralbio.2025.106373","url":null,"abstract":"<div><h3>Objective</h3><div>Recurrent aphthous stomatitis (RAS) is a common inflammatory condition of the oral mucosa with a multifactorial etiology, in which cytokine dysregulation is believed to play a central role. However, studies evaluating interleukin (IL)-1β, IL-6, and IL-10 levels and their genetic variants in RAS have yielded inconsistent results, likely due to population heterogeneity and methodological differences.</div></div><div><h3>Materials and methods</h3><div>This systematic review synthesizes current evidence on the association between single nucleotide polymorphisms (SNPs) of <em>IL1β</em>, <em>IL6</em>, and <em>IL10</em> and susceptibility to RAS.</div></div><div><h3>Result</h3><div>Population-based studies and meta-analyses identified recurrent associations involving <em>IL1β</em> (rs1143634, rs16944), <em>IL6</em> (rs1800795), and <em>IL10</em> (rs1800896, rs1800872), although results varied across ethnic groups. These genetic variants may influence cytokine expression or secretion, thereby shaping the inflammatory response. Complementary bioinformatics analyses revealed rare missense variants<em>—IL1β</em> (rs1558783084, rs1273629321), <em>IL6</em> (rs569143621, rs767924065), and <em>IL10</em> (rs1674874269, rs1674734957)—with high predicted pathogenicity scores (cumulative score 4–5), yet exceedingly low allele frequencies (AF &lt; 0.0001) in global datasets.</div></div><div><h3>Conclusion</h3><div>These findings highlight the complex interplay between genetic predisposition and population-specific factors in RAS. Further large-scale, multiethnic studies integrating functional validation are required to elucidate the precise contribution of cytokine gene variants to disease pathogenesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106373"},"PeriodicalIF":2.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144861130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GDC-0449 suppresses odontogenic keratocyst aggressiveness in fibroblasts by upregulating SPARC via Hedgehog pathway inhibition","authors":"Jiafei Qu ,&nbsp;Yongqi Jiang ,&nbsp;Shian Wu ,&nbsp;Xiwen Chen ,&nbsp;Jiemei Zhai ,&nbsp;Jing Gao ,&nbsp;Yuanzhu Zhou ,&nbsp;Yingying Hong ,&nbsp;Jing Shen ,&nbsp;Defu Chen","doi":"10.1016/j.archoralbio.2025.106374","DOIUrl":"10.1016/j.archoralbio.2025.106374","url":null,"abstract":"<div><h3>Objective</h3><div>Odontogenic keratocysts (OKCs) are aggressive jaw lesions, either linked to Gorlin-Goltz syndrome or sporadic. We aimed to investigate the impact of the Hedgehog (Hh) pathway inhibitor GDC-0449 on OKC fibroblasts (OKC-Fs). Additionally, we explored whether secreted protein acidic and rich in cysteine (SPARC) was a novel Hh target and its association with OKC aggressiveness.</div></div><div><h3>Design</h3><div>Fibroblasts from four syndromic and four non-syndromic OKCs were isolated, with patched 1 (<em>PTCH1</em>) mutations found only in syndromic ones. Syndromic OKC-Fs with <em>PTCH1</em> mutations were treated with GDC-0449, using mutation-free non-syndromic OKC-Fs as controls. Wound healing and transwell assays assessed the migration and invasion of OKC-Fs. Real-time polymerase chain reaction (PCR), alkaline phosphatase staining, and tartrate-resistant acid phosphatase staining investigated osteogenic differentiation and osteoclastogenic effects. Real-time PCR and western blotting examined the correlation between SPARC and Hh signaling. <em>SPARC</em> siRNA treatment tested its effect on cell migration and invasion.</div></div><div><h3>Results</h3><div>GDC-0449 inhibited the migration, invasion, and osteoclastogenic ability, while promoting their osteogenic differentiation of syndromic OKC-Fs. Moreover, GDC-0449 inhibited Hh pathway by decreasing glioma-associated oncogene 1 (GLI1) expression in syndromic OKC-Fs harboring <em>PTCH1</em> mutations. Additionally, by suppressing Hh pathway, GDC-0449 upregulated SPARC expression, suggesting that Hh-GLI1 signaling exerts a negative regulatory effect on SPARC. Knockdown of SPARC promoted the migration and invasion of OKC-Fs, which could be reversed by GDC-0449.</div></div><div><h3>Conclusion</h3><div>GDC-0449 suppresses aggressiveness and osteoclastogenesis of OKCs while promoting osteogenesis. GDC-0449 treats OKCs by inhibiting Hh signaling and upregulating SPARC. SPARC could be a potential therapeutic target for OKCs.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106374"},"PeriodicalIF":2.1,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A case of impacted third molar from the prehistoric Hypogeum of Calaforno (Giarratana, Ragusa, Sicily): Reflections on the antiquity and evolutionary implications of this trait","authors":"Elena Varotto ,&nbsp;Marta Kurek ,&nbsp;Pietro Maria Militello ,&nbsp;Erica Platania ,&nbsp;Francesco M. Galassi","doi":"10.1016/j.archoralbio.2025.106371","DOIUrl":"10.1016/j.archoralbio.2025.106371","url":null,"abstract":"<div><h3>Objectives</h3><div>This study examines a rare case of an impacted third molar identified in a fragmented right mandibular hemiarch from the prehistoric Hypogeum of Calaforno (Giarratana, Ragusa, Sicily, Italy), dating to the Late Eneolithic period (2600–2300 BC).</div></div><div><h3>Design</h3><div>A detailed osteological examination and palaeoradiological imaging were employed to analyze the mandibular fragment, leading to the identification of a horizontally impacted third molar pressing against the second molar.</div></div><div><h3>Results</h3><div>The analyses revealed clear evidence of a horizontally impacted third molar, contributing to the limited bioarchaeological record of dental impactions in ancient populations. The findings are interpreted within the broader evolutionary history of third molar impactions, considering genetic, developmental, and dietary factors as potential influences. The study also underscores the value of incorporating modern radiological methods into archaeological research.</div></div><div><h3>Conclusions</h3><div>This case provides new perspectives on the antiquity and potential prevalence of impacted third molars in ancient populations, suggesting that their apparent rarity in the archaeological record may be due to methodological challenges rather than an actual absence.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106371"},"PeriodicalIF":2.1,"publicationDate":"2025-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144895040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Fusobacterium nucleatum in the pathogenesis of oral squamous cell carcinoma: A systematic review of molecular mechanisms and signalling pathways","authors":"Bara’a El-Dwaikat ,&nbsp;Ikhlas El Karim ,&nbsp;Daniel B. Longley ,&nbsp;Paul N. Moynagh ,&nbsp;Fionnuala T. Lundy","doi":"10.1016/j.archoralbio.2025.106369","DOIUrl":"10.1016/j.archoralbio.2025.106369","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the mechanisms and signalling pathways involved in the pathogenesis of oral squamous cell carcinoma (OSCC) that are promoted by <em>Fusobacterium nucleatum</em> infection across human participant, <em>in vivo</em> and <em>in vitro</em> studies.</div></div><div><h3>Design</h3><div>This systematic review was conducted in accordance with the PRISMA guidelines to address the question: What mechanisms and potential signalling pathways are implicated in the pathogenesis of OSCC facilitated by <em>F. nucleatum</em> infection, compared with non-infected controls? Searches were performed across three electronic databases: Scopus, Web of Science, and MEDLINE.</div></div><div><h3>Results</h3><div>Sixty-three studies met the inclusion criteria for the systematic review. Studies involving human participants revealed alterations in bacterial genes related to lipopolysaccharide (LPS) synthesis, bacterial mobility and flagellar assembly. Additionally, alteration in host genes including DNA repair, tumour protein P53 (TP53), toll-like receptors and proinflammatory genes such as interleukin (IL)1β, IL8, and IL6 were reported. <em>In vivo</em> studies reported upregulation of cyclin D1 and IL6 following <em>F. nucleatum</em> infection. <em>In vitro</em> studies demonstrated changes in epithelial-mesenchymal transition markers such as E-cadherin, N-cadherin, vimentin, and zinc finger E-box binding homeobox (ZEB)1/2, along with increased expression of inflammatory markers, including IL-8 and IL-6 following <em>F. nucleatum</em> infection.</div></div><div><h3>Conclusion</h3><div>The findings from this systematic review highlight a significant molecular response to <em>F. nucleatum</em> infection in oral cancer. The results underscore the complex interaction between <em>F. nucleatum</em> and host molecular pathways, offering valuable insights into how this bacterium may contribute to oral cancer development and progression.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"179 ","pages":"Article 106369"},"PeriodicalIF":2.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144840924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The salivary and GCF AGE/sRAGE-IL-17 axis in periodontitis and diabetes: A pathophysiological perspective on disease progression and inflammation","authors":"Alper Aydoğdu ,&nbsp;Yagmur Deniz Yıldırım ,&nbsp;Banu Ertürk ,&nbsp;Begüm Cicek ,&nbsp;Deniz Cagdas ,&nbsp;Uğur Ünlütürk ,&nbsp;Ezel Berker","doi":"10.1016/j.archoralbio.2025.106370","DOIUrl":"10.1016/j.archoralbio.2025.106370","url":null,"abstract":"<div><h3>Objective</h3><div>Diabetes, if uncontrolled, increases the risk of periodontal disease and associated tooth loss. While AGE and sRAGE levels have been explored in diabetes, their role in localized inflammation in periodontium is poorly understood. The aim of this study was to investigate AGE/sRAGE ratio and IL-17 in saliva&amp;gingival crevicular fluid in the presence of periodontitis in diabetic patients which may underlie disease development or progression.</div></div><div><h3>Design</h3><div>This cross-sectional study included 64 patients, divided into 4 groups as healthy (H), periodontitis without diabetes(P), periodontitis patients with controlled diabetes(DP), periodontitis patients with poorly controlled diabetes(PDP). Periodontal clinical parameters were recorded. AGE, sRAGE, IL-17 levels in GCF and saliva samples were measured with ELISA and AGE/sRAGE ratio was determined.</div></div><div><h3>Results</h3><div>GCF AGE/sRAGE ratio and IL-17 levels of periodontitis groups were significantly higher than H group, while sRAGE levels were significantly lower (p &lt; 0.05). In addition, AGE/sRAGE ratio of PDP group were significantly higher than DP group in GCF and saliva (p &lt; 0.05). ROC curve analysis showed significantly discriminative performance of the parameters in both GCF and saliva. HbA1c levels were positively correlated with AGE/sRAGE ratio in GCF and saliva(p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>This study demonstrated that GCF and salivary biomarkers, particularly IL-17 and AGE/sRAGE ratio, may have diagnostic potential in distinguishing periodontal disease severity in relation to glycemic status. ROC analysis supported the discriminative ability of these markers, especially in GCF, with satisfactory sensitivity and specificity. The AGE/sRAGE ratio in both GCF and saliva showed promise as a non-invasive biomarker, especially in patients with poorly controlled diabetes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106370"},"PeriodicalIF":2.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144826879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microecological community- Streptococcus mutans dysbiosis and interaction provide therapeutic perspectives for dental caries","authors":"Yue Zhou,&nbsp;Chenchen Zhang,&nbsp;Yalan Deng,&nbsp;Lei Lei,&nbsp;Tao Hu","doi":"10.1016/j.archoralbio.2025.106367","DOIUrl":"10.1016/j.archoralbio.2025.106367","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to provide an overview of the dysbiosis and interaction between <em>Streptococcus mutans</em> (<em>S. mutans</em>) and other <em>Streptococci</em>, <em>Veillonella</em> spp., <em>Lactobacillus</em> spp., and <em>Candida albicans</em> in the oral cavity, which is a major driver of cariogenicity.</div></div><div><h3>Design</h3><div>The search for this narrative review was conducted in databases including PubMed, Web of Science, and Google Scholar, employing keywords like \"Dental caries,\" \"<em>Streptococcus mutans</em>,\" \"Commensal <em>Streptococci</em>,\" \"<em>Veillonella</em>,\" \"<em>Lactobacillus</em>,\" \"<em>Candida albicans</em>,\" and \"Interaction\" while manually retrieving the reference lists of journal articles.</div></div><div><h3>Results</h3><div>Dental caries has a high prevalence and low treatment rate in the population, which poses a great burden to public health and the social economy. The etiology of dental caries is closely linked to the imbalance of oral microbial communities. <em>S. mutans</em> is the major pathogen of dental caries. The cariogenic mechanism of <em>S. mutans</em> is primarily related to acid production and acid resistance, as well as polysaccharide production, adhesion, colonization, and the formation of cariogenic biofilm. However, there are complex interactions between <em>S. mutans</em> and other symbiotic microorganisms in the oral cavity, which synergistically or antagonistically affect the pathogenicity of microorganisms.</div></div><div><h3>Conclusion</h3><div>The interactions between <em>S. mutans</em> and oral commensal microorganisms on the microecology provide an in-depth understanding of the etiology of cariogenicity and new pathways for multiple caries prevention and treatment, such as hydrogen peroxide, arginine, farnesol, and probiotics.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106367"},"PeriodicalIF":2.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144827303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transplanted mesenchymal stem cells isolated from the bone marrow ameliorate the histological alterations in the parotid glands of hypothyroid male rats","authors":"Hanan El-Hemedy ,&nbsp;Mohamed Badawy ,&nbsp;Mary Moheb ,&nbsp;Maha El Shahawy","doi":"10.1016/j.archoralbio.2025.106368","DOIUrl":"10.1016/j.archoralbio.2025.106368","url":null,"abstract":"<div><h3>Objective</h3><div>To elucidate the possible outcome of mesenchymal stem cells extracted from the bone marrow (BM-MSCs) on the altered histological structure of the parotid gland of male rats with induced hypothyroidism.</div></div><div><h3>Design</h3><div>24 adult male Wistar rats were used. The rats were divided into 3 groups, each containing 8 animals. The control group contained the sham-operated animals. The hypothyroid rat group (HT group) contained animals receiving carbimazole at a dose of 20 mg/kg per day up to 6 weeks to induce hypothyroidism. The third group contained rats with induced hypothyroidism and were administered BM-MSCs via the tail vein (HT-MSC) group. BM-MSCs were extracted from 3-week-old rats and were immunophenotyped prior to injection to the HT-MSC group. The parotid glands were dissected 6 weeks post injection and processed to assess PKH67-labelled cells, histomorphometry and staining with Haematoxylin and Eosin (H and E).</div></div><div><h3>Results</h3><div>Rats with induced hypothyroidism depicted a significant decrease in the thyroid hormones´ serum levels. Extracted BM-MSCs were CD105 + , CD90 + and CD45^-. The parotid gland of HT group depicted an abnormal structure of the acini, intercalated, striated and excretory ducts including nuclear alterations, vacuolization and indistinct boundaries compared to their controls. In addition, the area and perimeter of the acini were diminished. The HT-MSCs group depicted green PKH67⁺-labelled MSCs, restoration of the normal acinar and ductal configuration, and regular area and perimeter of the acini.</div></div><div><h3>Conclusion</h3><div>Transplanted BM-MSCs resumed the normal parotid gland acinar, intercalated, striated and excretory duct structure in the hypothyroid male rats, suggesting restored tissue function.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106368"},"PeriodicalIF":2.1,"publicationDate":"2025-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144828742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between electronic nicotine delivery systems and dental caries: A systematic review with meta-analysis","authors":"Matheus dos Santos Fernandez ,&nbsp;Francisco Wilker Mustafa Gomes Muniz ,&nbsp;Felipe Gomes Dallepiane ,&nbsp;Nathalia Ribeiro Jorge da Silva-Garcia ,&nbsp;Mateus Andrade Rocha ,&nbsp;Andreia Morales Cascaes ,&nbsp;Ariadne Cristiane Cabral Cruz ,&nbsp;Maísa Casarin","doi":"10.1016/j.archoralbio.2025.106366","DOIUrl":"10.1016/j.archoralbio.2025.106366","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the association between the electronic nicotine-delivery systems (ENDS) use and dental caries in adolescents and adults.</div></div><div><h3>Material and methods</h3><div>Two researchers independently conducted the search across six databases, study selection, data extraction, and assessment of risk of bias and certainty of evidence. Included studies involved participants ≥ 16 years old, assessed ENDS use, and employed clinical and/or radiographic methods for caries diagnosis. Risk of bias was evaluated using the Joanna Briggs Institute Checklist and the Newcastle–Ottawa Scale. A meta-analysis was performed to calculate the mean difference (MD) in caries severity between ENDS users and non-users. The GRADE approach was used to assess the certainty of the evidence.</div></div><div><h3>Results</h3><div>Of the 559 eligible studies, four were included (three cross-sectional and one was a prospective cohort). Three studies identified a potential association between the ENDS use and either the experience or risk of dental caries, as well as the prevalence of untreated teeth. Three studies were classified as having a high risk of bias. The quantitative analysis of the pooled effects (2 studies) showed no significant MD between the history of dental caries among those who did or did not use ENDS (MD: 0.72, 95 % confidence interval: −1.70–3.15).</div></div><div><h3>Conclusion</h3><div>It was showed a potential association between ENDS use and increased severity and risk of dental caries in both population groups. However, quantitative analysis showed disease severity is not associated with the use of these devices among adults. The certainty of evidence for qualitative and quantitative analyses was very low.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106366"},"PeriodicalIF":2.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144749343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taiwanese green propolis suppresses NLRP3 inflammasome activation and induces Nrf2/HO-1 pathway to reduce periodontal pathogen-induced endothelial inflammation","authors":"Yi-Jen Lai ,&nbsp;Yin-Chu Chuang ,&nbsp;Yung-Li Wang ,&nbsp;Ming-Tse Li ,&nbsp;Emily Sunny Lee ,&nbsp;Wei-Ju Lee ,&nbsp;Wei-Ning Lin ,&nbsp;Yuh-Lien Chen ,&nbsp;Ching-Shuen Wang ,&nbsp;Thi Thuy Tien Vo ,&nbsp;Yue-Wen Chen ,&nbsp;I-Ta Lee","doi":"10.1016/j.archoralbio.2025.106364","DOIUrl":"10.1016/j.archoralbio.2025.106364","url":null,"abstract":"<div><h3>Objective</h3><div>Periodontitis is a chronic inflammatory disease associated with systemic conditions, including cardiovascular diseases. <em>Porphyromonas gingivalis</em> (Pg), a key periodontal pathogen, contributes to vascular endothelial dysfunction through its virulence factors. This study aimed to investigate the protective effects of Taiwanese green propolis (TGP) against Pg-derived lipopolysaccharide (Pg-LPS)-induced endothelial inflammation, focusing on its modulation of the NLRP3 inflammasome and Nrf2/HO-1 signaling pathways.</div></div><div><h3>Design</h3><div>Human aortic endothelial cells (HAECs) were stimulated with Pg-LPS in the presence or absence of TGP. The expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α) was assessed using real-time PCR and ELISA. ROS production was evaluated using fluorescence-based assays, while NF-κB activation and Nrf2 transcriptional activity were analyzed via luciferase reporter assays. Pharmacological inhibitors were used to confirm the involvement of these pathways.</div></div><div><h3>Results</h3><div>TGP significantly reduced Pg-LPS-induced IL-1β, TNF-α, and IL-6 expression in HAECs. It inhibited NF-κB activation, suppressed ROS generation, and attenuated NLRP3 inflammasome activation. Additionally, TGP upregulated HO-1 expression and enhanced Nrf2 transcriptional activity, as evidenced by ARE-driven luciferase reporter assays. Pharmacological inhibition of Nrf2 and HO-1 reversed TGP’s anti-inflammatory effects, confirming that the Nrf2/HO-1 axis is critical for its protective function.</div></div><div><h3>Conclusions</h3><div>These findings demonstrate that TGP exerts anti-inflammatory and cytoprotective effects by suppressing NLRP3 inflammasome activation and enhancing the Nrf2/HO-1 pathway, reducing Pg-LPS-induced endothelial inflammation. This study suggests that TGP could be a promising natural therapeutic agent for mitigating periodontal pathogen-induced systemic inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106364"},"PeriodicalIF":2.1,"publicationDate":"2025-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144756875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}