Archives of oral biology最新文献

{"title":"Evaluation of salivary melatonin and MMP-9 levels in periodontal diseases","authors":"Ali Batuhan Bayırlı ,&nbsp;Ceyda Gürhan ,&nbsp;Ercan Saruhan","doi":"10.1016/j.archoralbio.2024.106116","DOIUrl":"10.1016/j.archoralbio.2024.106116","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to evaluate salivary matrix metalloproteinase-9 (MMP-9) and melatonin levels in individuals with periodontal health, gingivitis, and periodontitis.</div></div><div><h3>Design</h3><div>A total of 170 participants were enrolled in this study. They included 57 periodontally healthy individuals, 58 gingivitis patients, and 55 periodontitis patients. Saliva samples were collected by passive drool technique. The levels of MMP-9 and melatonin in saliva were measured biochemically using the ELISA method.</div></div><div><h3>Results</h3><div>Salivary MMP-9 levels in the periodontitis group were significantly higher than those in the gingivitis and periodontally healthy groups, while salivary melatonin levels were significantly lower (<em>p</em>&lt;0.001). A positive correlation was observed between clinical periodontal parameters and salivary MMP-9 levels, while salivary melatonin levels were negatively correlated (<em>p</em>&lt;0.001). A negative correlation was also observed between salivary MMP-9 levels and salivary melatonin levels (<em>p</em>&lt;0.001).</div></div><div><h3>Conclusion</h3><div>This study shows that the level of melatonin in saliva is associated with periodontal disease and with the level of MMP-9 in saliva, which plays a role in this disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106116"},"PeriodicalIF":2.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking and in vitro validating chronic inflammation in human gingival fibroblasts","authors":"Anne Eriksson Agger ,&nbsp;Athina Samara ,&nbsp;Tianxiang Geng ,&nbsp;Ole Kristoffer Olstad ,&nbsp;Janne Elin Reseland","doi":"10.1016/j.archoralbio.2024.106113","DOIUrl":"10.1016/j.archoralbio.2024.106113","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to identify and validate in vitro conditions that may mimic the translational, cytokine and chemokine profiles observed in human inflamed gingiva in vivo.</div></div><div><h3>Design</h3><div>Primary human gingiva fibroblast cells (HFIB-G) were cultured under serum starvation conditions (0 – 10 %), supplemented with increasing lipopolysaccharide (LPS) concentrations (0.1, 1, or 10 µg/ml) from two bacterial strains <em>E. coli</em> and <em>P. gingivalis</em> and 0.1, 1, or 10 ng/ml recombinant interleukin 1β (IL-1β), alone or in combinations. The levels of cytokines/chemokines were measured in the cell culture medium by Luminex, and gene expression was quantified by Affymetrix microarrays at 24, 48 and 72 h.</div></div><div><h3>Results</h3><div>Inflammation markers were not elevated after stimulation with <em>P. gingivalis</em> LPS, while <em>E. coli</em> LPS and IL-1β individually increased the secretion of interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) to the cell culture medium. IL-1β administration also increased the secretion of several factors, including tumor necrosis factor (TNFα). However, the combination of 1 µg/ml <em>E. coli</em> LPS, 1 ng/ml IL-1β and serum starvation led to increased secretion of IL-6, TNFα, in addition to other factors found in inflamed tissue. Gene expression analyses revealed that this combination not only enhanced the expression interleukins/chemokines genes but also T helper cell signaling and matrix metalloproteinases.</div></div><div><h3>Conclusion</h3><div>Serum reduction in cell culture medium together with the administration of <em>E. coli</em> LPS and IL-1β resulted in gene expression and secreted cytokine/chemokine profiles similar to that found in vivo during chronic inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106113"},"PeriodicalIF":2.2,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of botulinum toxin type A on tooth movement and bone remodeling in male Wistar rats","authors":"Joana Estephany Gordillo Yépez ,&nbsp;Renata Machado Marangon ,&nbsp;Aline Cristina Batista Rodrigues Johann ,&nbsp;Bruno Massa de Viveiros ,&nbsp;Patricia Kern Di Scala Andreis ,&nbsp;Luana Vosgerau ,&nbsp;Sara Moreira Leal Salvação ,&nbsp;Orlando Motohiro Tanaka ,&nbsp;Odilon Guariza-Filho ,&nbsp;Sergio Aparecido Ignácio ,&nbsp;Elisa Souza Camargo","doi":"10.1016/j.archoralbio.2024.106105","DOIUrl":"10.1016/j.archoralbio.2024.106105","url":null,"abstract":"<div><h3>Objective</h3><div>We evaluated whether the use of botulinum neurotoxin type A (BTX-A) in masticatory muscles influences tooth movement and bone remodeling.</div></div><div><h3>Design</h3><div>Seventy-seven male Wistar rats were allocated to the groups: S - Saline (n=20); SM - Saline with movement (n=20); BT - Botulinum toxin (n=18); BTM - Botulinum toxin with movement (n=19). On day 1, 0.02 mL of sterile 0.9 % saline was administered to groups S and SM and BTX-A (1 U in 0.02 mL of saline) to groups BT and BTM, in the masseter and temporal muscles laterally. On day 30, a nickel titanium spring was installed to move the first maxillary molar and euthanasia was performed on days 32 and 51. Tooth displacement, maxillary and mandibular bone volumes, collagen neoformation, bone and root resorptions, and masseter morphometry were assessed. Statistical analysis was conducted (<em>p</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>A higher percentage of type I collagen was observed in the BT than in the S group on day 51 and lower mass, length, and diameter of the masseter fibers in BT and BTM (<em>p</em> &lt; 0.05). Tooth displacement, bone volume, bone and root resorptions, hyaline area, and masseter height showed no difference among groups with and without BTX-A, regardless of tooth movement (<em>p</em> &gt; 0.05).</div></div><div><h3>Conclusions</h3><div>BTX-A did not interfere with tooth displacement, bone volume, and dental and periodontal tissues related to tooth movement in rats; it increased mature collagen in animals without tooth movement; and it caused a decrease in the mass, length, and diameter of the masseter fibers.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106105"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography","authors":"Didem Sakaryalı Uyar ,&nbsp;Hazal Karslıoğlu ,&nbsp;Mert Ocak ,&nbsp;Hakan Hamdi Çelik","doi":"10.1016/j.archoralbio.2024.106111","DOIUrl":"10.1016/j.archoralbio.2024.106111","url":null,"abstract":"<div><h3>Objectives</h3><div>To determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth.</div></div><div><h3>Design</h3><div>This study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>There was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p &lt; 0.05) and middle (p = 0.010; p &lt; 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p &lt; 0.001; p &lt; 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>There were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106111"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of dental chipping for identifying and diagnosing tooth fracture patterns in osteological series","authors":"Á. Rubio Salvador ,&nbsp;S.A. Jiménez-Brobeil ,&nbsp;M. Lozano","doi":"10.1016/j.archoralbio.2024.106114","DOIUrl":"10.1016/j.archoralbio.2024.106114","url":null,"abstract":"<div><h3>Objective</h3><div>To develop a specific methodology for identifying dental chipping and determining its temporal occurrence in past populations.</div></div><div><h3>Design</h3><div>The analysed sample comprised of 2191 human teeth from various Bronze Age on the Iberian Peninsula (Argar culture, 1900–1450 cal BC). Among these, 471 chipped teeth were identified. Chipping was examined using various microscopic techniques (digital three-dimensional, optical, and confocal), focusing on distribution, morphology, position in the tooth, extent of damage, and post-chipping antemortem modifications (PCAM).</div></div><div><h3>Results</h3><div>The distribution and morphology of the chips enabled the identification chipping mechanism of the chipping, providing valid criteria to distinguish between antemortem and postmortem chipping. Microscopic analyses of the chipping segments—edges, sidewalls, surface, and surrounding area—facilitated determination of the time the chip ocurred (antemortem: recent, less recent, or not recent).</div></div><div><h3>Conclusions</h3><div>While experimental studies provide valuable insights into chipping mechanisms, many criteria may not be applicable to past populations because of the presence of PCAM. The lack of PCAM in some Argaric teeth suggests that previous studies may have underestimated the prevalence of chipping in past populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106114"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of icariin on dental pulp stem cells and its potential applications in dentin repair","authors":"Ahmed Elhakim ,&nbsp;Ukseong Kim ,&nbsp;Euiseong Kim ,&nbsp;Sukjoon Lee ,&nbsp;Jong-Min Lee ,&nbsp;Han-Sung Jung ,&nbsp;Sunil Kim","doi":"10.1016/j.archoralbio.2024.106112","DOIUrl":"10.1016/j.archoralbio.2024.106112","url":null,"abstract":"<div><h3>Objectives</h3><div>As dental pulp therapy evolves towards regenerative approaches, biomolecules such as icariin, derived from <em>Epimedium</em> flowers, are being evaluated for their therapeutic potential. This study investigates icariin's effectiveness in promoting odontogenic differentiation in human dental pulp stem cells (hDPSCs) <em>in vitro</em> and as a pulp-capping agent <em>in vivo</em>.</div></div><div><h3>Design</h3><div>The study explored the effects of icariin on hDPSCs at concentrations of 10, 20, and 40 µM. Cell viability and migration assays were conducted to evaluate cytotoxicity and chemotaxis. Odontogenic differentiation was assessed using alkaline phosphatase staining and alizarin red S (ARS) staining, complemented by real-time PCR and Western blot analyses of key markers such as RUNX family transcription factor 2 (<em>RUNX2</em>), collagen type I alpha 1 chain (<em>COL1A1</em>), alkaline phosphatase (<em>ALPL</em>), and dentin sialophosphoprotein (<em>DSPP</em>). Additionally, the <em>in vivo</em> effects of icariin were tested in a rat maxillary molar model, where icariin-treated collagen sponges were used for direct pulp capping to evaluate its potential to induce reparative dentin formation.</div></div><div><h3>Results</h3><div>Icariin showed no cytotoxic effects on hDPSCs at any tested concentration, enhanced migratory activity in a dose-dependent manner, and significantly increased alkaline phosphatase activity and calcium deposition. Gene and protein expression analyses revealed a dose-dependent increase in odontogenic differentiation markers in icariin-treated hDPSCs. <em>In vivo</em>, icariin effectively promoted reparative dentin formation in exposed rat pulp.</div></div><div><h3>Conclusions</h3><div>Icariin enhances odontogenic differentiation of hDPSCs and has promising potential as a pulp-capping agent for vital pulp therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106112"},"PeriodicalIF":2.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA damage and cell death in human oral squamous cell carcinoma cells: The potential biological effects of cannabidiol","authors":"Monia Billi ,&nbsp;Stefano Pagano ,&nbsp;Gian Luca Pancrazi ,&nbsp;Chiara Valenti ,&nbsp;Stefano Bruscoli ,&nbsp;Alessandro Di Michele ,&nbsp;Marta Febo ,&nbsp;Francesco Grignani ,&nbsp;Lorella Marinucci","doi":"10.1016/j.archoralbio.2024.106110","DOIUrl":"10.1016/j.archoralbio.2024.106110","url":null,"abstract":"<div><h3>Objective</h3><div>The present study examined the in vitro effects on oral squamous cell carcinoma cells (HSC-3) of cannabidiol (CBD), the main chemical component of Cannabis, proposed as a novel adjuvant therapy in the treatment of cancers.</div></div><div><h3>Design</h3><div>Cell viability (MTT assay), morphology (SEM), apoptosis and cell cycle (flow cytometry), and DNA damage (phospho-γ-H2AX immunofluorescence) were evaluated. Cytotoxicity was evaluated with concentrations between 100 µM and 1 µM, and two concentrations were selected for subsequent analysis: 25 µM, as toxic dose, and 6.25 µM, as non-toxic.</div></div><div><h3>Results</h3><div>CBD caused a dose- and time-dependent reduction in viability of 64 %, 96 %, and 99 % with 25 µM, 50 µM and 100 µM, respectively, after 72 h (p &lt; 0.001), cell cycle arrest in G0-G1 phase with increased apoptosis in particular at 72 h for 25 µM (p &lt; 0.001), significant morphological alterations with 25 µM, still present even at 6.25 µM, and significantly increased cell damage considering a significant increase in the percentage of highly positive cells (5 phosphorylated γH2AX foci), which is around 29 % for 25 µM and 19 % for 6.25 µM after 24 h.</div></div><div><h3>Conclusions</h3><div>CBD inhibits oral cancer growth causing DNA damage. In general, induced cell cytotoxicity appears to be dose- and time-related. Doses of CBD ≥25 μM showed a high reduction in viability. CBD could possibly represent a new therapeutic molecule for its cytotoxic effects against oral squamous cell carcinoma. The mechanism involved in the suppressive effect caused by CBD needs further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106110"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melanoma-inhibiting activity promotes the migration and odontoblastic differentiation of stem cells of apical papilla","authors":"Huihui Ren ,&nbsp;Qingxuan Zhao ,&nbsp;Nan Wang ,&nbsp;Xiaojing Yuan ,&nbsp;Rui Song ,&nbsp;Quan Wen ,&nbsp;Yuming Zhao","doi":"10.1016/j.archoralbio.2024.106109","DOIUrl":"10.1016/j.archoralbio.2024.106109","url":null,"abstract":"<div><h3>Objective</h3><div>Melanoma Inhibitory Activity (MIA) has been predominantly studied in the context of melanoma and cartilage development. However, its role in dental pulp development and stem cell behavior remains largely unexplored. This study investigates the expression pattern of MIA in dental pulp tissues and its potential role in the proliferation, migration, and odontoblastic differentiation of stem cells from the apical papilla (SCAPs).</div></div><div><h3>Design</h3><div>MIA expression in human pulp tissue was demonstrated by immunohistochemistry. SCAPs were cultured in normal and mineralization induction media, with MIA levels monitored via RT-qPCR and Western blot. Cell proliferation was evaluated using the CCK8 assay, while transwell and cell scratch assays were conducted to examine cell migration. The effect of MIA on odontoblastic differentiation was examined by qRT-PCR, Alkaline phosphatase activity assay, and Western blot. siRNA was used to knock down MIA to investigate its effect. A mouse subcutaneous implantation model was used to assess whether MIA promotes odontoblastic differentiation <em>in vivo</em>.</div></div><div><h3>Results</h3><div>MIA expression was observed in the papilla and odontoblasts layer of the developing pulp. <em>In vitro</em>, MIA expression increased during SCAPs differentiation and was found to significantly enhance migration, and odontoblastic differentiation but not proliferation. Gene knockdown experiments confirmed MIA’s pivotal role in promoting SCAPs migration and differentiation. <em>In vivo</em>, MIA facilitated the formation of dentin-like structures and enhanced pulp-dentin complex regeneration.</div></div><div><h3>Conclusion</h3><div>MIA plays a crucial role in SCAPs’ migration and differentiation, suggesting its potential application in pulp-dentin regeneration therapies. Further studies are required to fully elucidate the underlying mechanisms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106109"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D serum levels and temporomandibular disorders: A systematic review and meta-analysis","authors":"Reza Tabrizi ,&nbsp;Hooman Khanzadeh ,&nbsp;Seyed Sepehr Mirebeigi Jamasbi ,&nbsp;Fatemeh Rezaei ,&nbsp;Ali Azadi","doi":"10.1016/j.archoralbio.2024.106108","DOIUrl":"10.1016/j.archoralbio.2024.106108","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review evaluates the connection between vitamin D serum levels, deficiency, and temporomandibular disorders (TMD), offering a meta-analysis of the existing evidence in this domain.</div></div><div><h3>Design</h3><div>The Scopus, ISI Web of Science, and Pubmed databases were searched for human studies concerning the connection between vitamin D and TMD comprising a control group. A random-effect model with forest plots was used for vitamin D serum levels mean difference (MD), vitamin D deficiency odds ratio (OR), and risk difference (RD) between subjects with and without TMD. Subgroup analysis was conducted based on ethnicity, overall risk of bias, TMD diagnosis method, and study designs. A p-value lower than 0.05 was considered significant. The certainty of the meta-evidence was evaluated according to the GRADE approach.</div></div><div><h3>Results</h3><div>Of the 2621 identified unique records, 15 studies were included in the study, eight of which were considered for the meta-analysis. The meta-analysis revealed a significant vitamin D deficiency OR (3.85; 95 % CI: 2.35 – 5.43; Certainty: Low) and RD (22 %; 95 % CI: 11 % - 32 %; Certainty: Very low), and vitamin D serum levels MD (-5.03 ng/mL; 95 % CI: −9.92 – −0.13; Certainty: Very low) between subjects with and without TMD. Among subgroup analyses, only the difference in vitamin D MD between Middle Eastern and European patients was significant (<em>P</em> &lt; 0.01).</div></div><div><h3>Conclusion</h3><div>Considering the low to very low certainty of the evidence, vitamin D serum levels are significantly lower, and vitamin D deficiency is significantly more prevalent in TMD patients.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106108"},"PeriodicalIF":2.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between polymorphisms in TNF-⍺ and RANKL genes is associated with the development of persistent apical periodontitis, in Brazilian subjects","authors":"Igor Bassi Ferreira Petean ,&nbsp;Alice Corrêa Silva-Sousa ,&nbsp;Guido Artemio Marañón-Vásquez ,&nbsp;Francisco Wanderley Garcia de Paula-Silva ,&nbsp;Erika Calvano Küchler ,&nbsp;Leonardo Santos Antunes ,&nbsp;Raquel Assed Bezerra Segato ,&nbsp;Lea Assed Bezerra da Silva ,&nbsp;Jardel Francisco Mazzi-Chaves ,&nbsp;Fabiane Carneiro Lopes-Olhê ,&nbsp;Manoel Damião Sousa-Neto","doi":"10.1016/j.archoralbio.2024.106106","DOIUrl":"10.1016/j.archoralbio.2024.106106","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the association between genetic polymorphisms in suppressor of cytokine signaling-1 (<em>SOCS-1</em>)<em>,</em> tumor necrosis factor-⍺ (<em>TNF-α</em>) and its receptors 1 and 2 (<em>TNFRSF1A</em> and <em>TNFRSF1B</em>), receptor activator of nuclear factor kappa-b (<em>RANK</em>), receptor activator of nuclear factor-kappa B ligand (<em>RANKL</em>) and osteoprotegerin (<em>OPG</em>), and persistent apical periodontitis (PAP).</div></div><div><h3>Methods</h3><div>Patients with pulp necrosis and apical periodontitis at the time of non-surgical root canal treatment were followed up for at least one year. A total of 423 subjects were included, 172 with signs/symptoms of PAP and 251 with apical periodontitis healed. DNA was extracted from saliva and used for genotyping polymorphisms in <em>SOCS1</em> (rs243327 and rs33977706), <em>TNF-α</em> (rs1800629), <em>TNFRSF1A</em> (rs1800693), <em>TNFRSF1B</em> (rs1061622), <em>RANK</em> (rs1805034), <em>RANKL</em> (rs1054016) and <em>OPG</em> (rs1032128) by real-time PCR. The frequency of genotypes and alleles was assessed using chi-squared test or Fisher's exact test and odds ratio. Interactions were also tested using multifactor dimensionality reduction (<em>α</em> = 5 %).</div></div><div><h3>Results</h3><div>In the polymorphism rs1800629 in <em>TNF-</em>α, carrying at least one A risk allele significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.28–0.79; <em>p</em>=0.004). For the polymorphism rs1054016 in <em>RANKL</em> carrying both T risk alleles significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.24–0.92; <em>p</em>=0.027). None of the other polymorphisms evaluated were associated with PAP (<em>p</em>&gt;0.05). A strong interaction was observed among rs1800629, rs1061622 and rs1054016.</div></div><div><h3>Conclusions</h3><div>Genetic polymorphisms rs1800629 (<em>TNF-α)</em> and rs1054016 (<em>RANKL)</em> had a strong interaction and were associated with a lower risk to develop PAP.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106106"},"PeriodicalIF":2.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}