Archives of oral biology最新文献

{"title":"Experts' narrative review “Mastication, Hippocampal Structure Changes and Cognition”","authors":"Maria Grazia Piancino","doi":"10.1016/j.archoralbio.2026.106522","DOIUrl":"10.1016/j.archoralbio.2026.106522","url":null,"abstract":"<div><h3>Objective</h3><div>Recent evidence highlights a fundamental link between masticatory function and brain health. Once regarded solely as a peripheral motor activity for food processing and occlusal balance, mastication is now recognized as a key factor in maintaining and enhancing cognitive function across the lifespan.</div></div><div><h3>Design</h3><div>This narrative review was conducted using relevant keywords through searches in PubMed, Scopus, and Web of Science, as well as manual searching of the bibliographies of journal articles.</div></div><div><h3>Results</h3><div>Basic research has shown that chewing stimulates neurogenesis in the hippocampus, resulting in increased neuronal and synaptic density, as well as the upregulation of brain-derived neurotrophic factor (BDNF), which leads to improvements in memory and cognition. This effect has been documented in both animal and clinical research, particularly among the elderly, and is supported by data from national health programs, which indicate that adequate prosthodontic rehabilitation can help preserve cognitive function. Etiopathogenetic insights suggest that loss of posterior teeth, rather than overall tooth count, is particularly detrimental, as these teeth are essential for effective mastication. Proposed mechanisms involve exercise-induced myokines, such as Cathepsin B, and chewing-induced neprilysin production, which may mediate hippocampal neuroprotection.</div></div><div><h3>Conclusions</h3><div>Collectively, these findings support a paradigm shift: mastication should be promoted as a preventive strategy for both oral and neural health. Public health efforts and clinical practices should integrate education on maintaining posterior dentition, promoting diets with adequate texture, and supporting prosthetic rehabilitation to sustain neuromuscular activity, thereby protecting cognitive function from early development through old age.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"184 ","pages":"Article 106522"},"PeriodicalIF":2.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction notice to “p38 Mitogen-activated protein kinase modulates cisplatin resistance in head and neck squamous cell carcinoma cells” [Archives of Oral Biology 122 (2021)104981]","authors":"Shomereeta Roy ,&nbsp;Souvick Roy ,&nbsp;Kumari Anuja ,&nbsp;Shweta Thakur ,&nbsp;Yusuf Akhter ,&nbsp;Swatishree Padhi ,&nbsp;Birendranath Banerjee","doi":"10.1016/j.archoralbio.2025.106489","DOIUrl":"10.1016/j.archoralbio.2025.106489","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"184 ","pages":"Article 106489"},"PeriodicalIF":2.1,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146127788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the impact of postnatal maternal separation stress on enamel formation in an experimental murine model","authors":"Júlia I.T. Sousa ,&nbsp;Juliana L. Gonçalves ,&nbsp;Guido A. Marañón-Vásquez ,&nbsp;Roberta D. Leme ,&nbsp;Larissa S. Sales ,&nbsp;Fabrício K. Carvalho ,&nbsp;Alexandra M. Queiroz ,&nbsp;Alice Corrêa Silva-Sousa ,&nbsp;Manoel Damião de Sousa-Neto ,&nbsp;Francisco W.G. Paula-Silva","doi":"10.1016/j.archoralbio.2025.106487","DOIUrl":"10.1016/j.archoralbio.2025.106487","url":null,"abstract":"<div><h3>Background</h3><div>The adverse effects of postnatal maternal separation (MS) on offspring development are well documented; however, its specific impact on dental enamel formation remains inadequately explored. This study examines the effects of maternal-separation–induced toxic stress on dental enamel formation in a murine model. Methods: A total of 24 Wistar rat offspring (<em>Rattus norvegicus</em>) were utilized, divided into a control group (n = 12) and an MS group (n = 12). The MS group underwent 4 h of daily separation from their mothers from postnatal day 2 to day 21, while the control group remained undisturbed with their mothers. Throughout the experimental period, body weight, length, and key developmental milestones (including incisor eruption, eye opening, and ear opening) were monitored. At postnatal day 28, the animals were euthanized, and the incisors were collected for analysis. Incisor photographs were conducted for macroscopic evaluation. Volumetric assessment of the enamel layer was performed using micro-computed tomography (micro-CT) imaging. Microhardness testing quantified enamel resistance, while scanning electron microscopy (SEM) provided insights into the morphology and ultrastructure of incisor enamel. In addition, energy-dispersive X-ray spectroscopy (EDS) was performed to evaluate mineral content and identify possible compositional alterations in dental enamel. Data were statistically analyzed using Student's t-test (α = 0.05). Results: The findings revealed an increase in body weight within the MS group (<em>p</em> &lt; 0.05), while normal growth and developmental milestones exhibited no significant changes (<em>p</em> &gt; 0.05). No macroscopic alterations were observed in the dental enamel of incisors of MS group. SEM analysis of the incisors indicated that the enamel structure of incisors in the MS group maintained an organized arrangement of enamel rods, characterized by densely packed, elongated structures extending from the underlying dentin to enamel surface. Furthermore, structural integrity, microhardness, and mineral composition remained largely unaffected (<em>p</em> &gt; 0.05). Conclusion: Under the given experimental conditions, postnatal maternal separation does not significantly compromise dental enamel formation in rat offspring.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106487"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and anti-virulence activity of juglone against Enterococcus faecalis compared with chlorhexidine","authors":"Rawan Yassin Nimir ,&nbsp;Homa Darmani","doi":"10.1016/j.archoralbio.2026.106511","DOIUrl":"10.1016/j.archoralbio.2026.106511","url":null,"abstract":"<div><h3>Objective(s)</h3><div>We compared the antimicrobial and antivirulence activities of juglone, a plant-derived naphthoquinone, with chlorhexidine (CHX), a widely used dental antiseptic, against <em>Enterococcus faecalis</em>, a pathogen associated with persistent oral and endodontic infections.</div></div><div><h3>Design</h3><div>Antimicrobial efficacy was evaluated using well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) assays. Possible interactions between juglone and CHX were investigated using well diffusion and checkerboard methods. Antivirulence activity was assessed by measuring secreted enzymes (caseinase, gelatinase, and hemolysin), biofilm formation, metabolic activity, extracellular polymeric substance (EPS) production, and disruption of mature biofilms. Oxidative stress responses were examined through lactate dehydrogenase (LDH) release, malondialdehyde (MDA) levels, superoxide dismutase (SOD) activity, and total antioxidant capacity (T-AOC).</div></div><div><h3>Results</h3><div>Juglone showed antibacterial activity with MICs in a similar range to CHX; however, no synergistic interaction between the two agents was observed. Juglone strongly inhibited caseinase, gelatinase, and hemolysin, whereas CHX showed no effect on caseinase and weaker inhibition of the others. CHX more effectively reduced early biofilm formation, metabolic activity, and mature biomass, whereas both agents similarly decreased EPS production and viability during biofilm development. Notably, in preformed biofilms, juglone at 16 ×MIC caused greater killing (∼4-log₁₀ CFU) than CHX (∼2-log₁₀). Both compounds increased LDH release and reduced SOD activity and T-AOC, while juglone uniquely decreased MDA levels, indicating additional redox-modulating effects.</div></div><div><h3>Conclusions</h3><div>Juglone impairs <em>E. faecalis</em> virulence by reducing enzymatic activity, oxidative stress tolerance, and biofilm viability, with stronger effects on established biofilms, highlighting its potential for further investigation in oral infection management.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106511"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145954223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral live microbial therapeutics: A synthetic biology roadmap toward durable oral microbiome-based therapies","authors":"Seok Bin Yang ,&nbsp;Kyu Hwan Kwack ,&nbsp;Jae-Hyung Lee ,&nbsp;Ji-Hoi Moon","doi":"10.1016/j.archoralbio.2026.106515","DOIUrl":"10.1016/j.archoralbio.2026.106515","url":null,"abstract":"<div><h3>Objectives</h3><div>Oral live microbial therapeutics (LMTs) show promise for halitosis, caries, and adjunctive periodontal care, yet benefits often fade after dosing stops. We synthesized evidence across indications and reframed development around quantifying and engineering persistence at intraoral sites, while outlining safety-by-design and delivery considerations for the oral niche.</div></div><div><h3>Design</h3><div>This narrative review integrated randomized trials, observational studies, and <em>in vitro/ex vivo</em> investigations to characterize clinical outcomes, persistence-related metrics, and engineering principles relevant to oral LMT development. Sources included PubMed/MEDLINE, Web of Science, Embase, and ClinicalTrials.gov, with backward/forward citation tracking. We included studies on LMTs in oral or gut contexts when mechanistically informative for oral applications (<em>e.g</em>., persistence, delivery, or biocontainment). Eligibility required clinical outcomes or persistence-related readouts. Two reviewers screened records and resolved disagreements by consensus. Reporting and assay principles were informed by STORMS and MIQE to support transparent, reproducible methods.</div></div><div><h3>Results</h3><div>Across indications, effects typically peak during dosing and attenuate after cessation, varying with strain, delivery format, and co-interventions (<em>e.g</em>., tongue dorsum debridement; standardized periodontal care). Persistence is rarely co-measured with clinical endpoints, limiting mechanistic interpretation. We outline a site-resolved measurement set, including time above the limit-of-detection, colonization area under the curve, apparent half-life (t½), and t½ under oral-mimetic shear, together with an engineering toolkit combining mucoadhesive/enamel-interactive carriers, single-cell coatings, and multilayer biocontainment (<em>e.g</em>., logic-gated/CRISPR kill switches, synthetic auxotrophy), and chemistry, manufacturing, and controls considerations.</div></div><div><h3>Conclusions</h3><div>Embedding persistence metrics and safety-by-design into study protocols may support more durable outcomes, and standardized, site-resolved reporting will be essential for clinical translation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106515"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High expression of a novel m6A reader LRPPRC predicts immunotherapy response and prognosis in head and neck cancer","authors":"Karpakavinayakam Gopalakrishnan ,&nbsp;Balachander Kannan ,&nbsp;Vijayashree Priyadharsini Jayaseelan ,&nbsp;Paramasivam Arumugam","doi":"10.1016/j.archoralbio.2026.106514","DOIUrl":"10.1016/j.archoralbio.2026.106514","url":null,"abstract":"<div><h3>Objective</h3><div>In this study, we examine the role of the leucine-rich pentatricopeptide repeat-containing gene (LRPPRC) as a new m6A reader in head and neck squamous cell carcinoma (HNSCC). We also analyze its expression pattern, prognostic significance, and response to immunotherapy.</div></div><div><h3>Design</h3><div>LRPPRC expression was comprehensively analyzed in HNSCC using data from The Cancer Genome Atlas Head-Neck Squamous Cell Carcinoma (TCGA-HNSC) and then validated in an independent patient cohort using real-time qPCR. The study further investigated the correlations between LRPPRC expression and methylation status, as well as their associations with clinicopathological features, patient prognosis, and immune profiles. Additionally, the impact of LRPPRC expression on the response to immunotherapy was assessed.</div></div><div><h3>Results</h3><div>We found that LRPPRC is significantly upregulated in HNSCC tissues and correlates with advanced clinicopathological features, nodal metastasis, and poor survival outcomes. In contrast, the DNA methylation level of <em>LRPPRC</em> was found to be reduced in HNSCC. Furthermore, <em>LRPPRC</em> expression is significantly correlated with infiltration of immune cells, specifically in CD4⁺ T cells, macrophages, and dendritic cells. Importantly, the expression of LRPPRC has been found to be a predictor of resistance to immunotherapy in patients with HNSCC.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that LRPPRC may serve as a promising biomarker for HNSCC prognosis and a therapeutic target for controlling cancer metastases and enhancing patient outcomes, while being a predictor for immune therapy response as well.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106514"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145973210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Odor characteristics and associated bacterial profiles in peri-implantitis using a novel odor measurement device","authors":"Tadahiro Kashiwamura ,&nbsp;Yusuke Taniguchi ,&nbsp;Ryutaro Ito ,&nbsp;Hirofumi Kido ,&nbsp;Kae Kakura ,&nbsp;Nao Suzuki","doi":"10.1016/j.archoralbio.2025.106486","DOIUrl":"10.1016/j.archoralbio.2025.106486","url":null,"abstract":"<div><h3>Objective</h3><div>This study assessed peri-implantitis risk by measuring odor using a novel device capable of identifying odorous components and bacteria specific to peri-implantitis.</div></div><div><h3>Design</h3><div>Implants from eight peri-implantitis patients and superstructures from 20 maintenance patients were evaluated using organoleptic testing and an odor detection device (nose@MEMS, I-PEX, Kyoto, Japan). In the maintenance group, 13 superstructures emitted odor, while 7 did not. Plaque samples were collected from peri-implant sites, and bacterial composition was analyzed via 16S rRNA sequencing.</div></div><div><h3>Results</h3><div>Principal component analysis of odor measurements obtained with the I-PEX system revealed that implants from peri-implantitis patients and odor-positive superstructures during maintenance formed a distribution distinct from odor-negative superstructures. Comparative analysis of bacterial composition between samples in the odor-associated region (Region A) and those including odor-negative superstructures (Region B) showed that Region A had a higher proportion of anaerobic bacteria, while Region B was enriched in commensal oral bacteria. Among 14 bacterial species previously linked to peri-implantitis, <em>Porphyromonas gingivalis</em>, <em>Tannerella forsythia</em>, and <em>Porphyromonas endodontalis</em> were significantly more abundant in Region A, whereas <em>Fretibacterium fastidiosum</em> and <em>Mogibacterium</em> were significantly lower.</div></div><div><h3>Conclusions</h3><div>The odor characteristics of peri-implantitis and odor-positive superstructures were similar and clearly distinguishable from odor-negative counterparts. The shared odor profile was associated with higher proportions of bacterial species previously linked to peri-implantitis. These findings imply that odor analysis using this novel device may provide a promising strategy for implant maintenance and peri-implantitis risk prediction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106486"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145919421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Changes in the craniofacial morphology in sclerostin knockout mice","authors":"Kazutaka Ikeda ,&nbsp;Masato Kaku ,&nbsp;Chao Fan-Yi ,&nbsp;Chisa Shukunami ,&nbsp;Kotaro Tanimoto","doi":"10.1016/j.archoralbio.2025.106492","DOIUrl":"10.1016/j.archoralbio.2025.106492","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the influence of lack of sclerostin on craniofacial morphology in sclerostin gene (<em>Sost</em>) knockout (<em>Sost</em>^{<em>Δ26/Δ26</em>}) mice.</div></div><div><h3>Design</h3><div>Micro computed tomography (micro-CT) was performed on both 60-day-old wild-type and <em>Sost</em>^{<em>Δ26/Δ26</em>} mice to analyze changes in craniofacial morphology. Ten anatomical landmarks and eight measurement parameters as well as the total mandibular volume were used to assess craniofacial features. In addition, the femora of both wild-type and <em>Sost</em>^{<em>Δ26/Δ26</em>} mice were examined using micro-CT and histological analysis. Cancellous bone volume, cortical bone volume, and the number of osteoclasts beneath the growth plate in the femora were evaluated.</div></div><div><h3>Results</h3><div>The neurocranium was significantly smaller in the <em>Sost</em>^{<em>Δ26/Δ26</em>} mice than in the wild-type mice, while the length of the angular process of the mandible was significantly greater. The total mandibular volume was significantly larger in the <em>Sost</em>^{<em>Δ26/Δ26</em>} mice than in the wild-type mice. The volume of cancellous bone and cortical bone in the femora were significantly larger in the <em>Sost</em>^{<em>Δ26/Δ26</em>} mice than in the littermate wild-type mice. Histological observation showed no statistically significant difference in the number of osteoclasts present in the femora between wild-type mice and <em>Sost</em>^{<em>Δ26/Δ26</em>} mice.</div></div><div><h3>Conclusions</h3><div>The present study showed a significant decrease in neurocranium and a significant increase in mandibular bone in <em>Sost</em>^{<em>Δ26/Δ26</em>} mice compared to wild-type mice, along with increased volumes of cancellous and cortical bone in the femora.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106492"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145881375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Association between agenesis of permanent teeth and other dental anomalies in nonsyndromic patients: Systematic review” [Archives of Oral Biology 173 (2025) 106223]","authors":"Paula Cristiana Santos de Aguiar ,&nbsp;Caio Melo Mesquita ,&nbsp;Fabiana Evangelista Lerner ,&nbsp;Laura Bezerra Borges ,&nbsp;Rodrigo Rodrigues ,&nbsp;Walbert A. Vieira ,&nbsp;Luiz Renato Paranhos ,&nbsp;Rui Barbosa de Brito Júnior","doi":"10.1016/j.archoralbio.2025.106490","DOIUrl":"10.1016/j.archoralbio.2025.106490","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106490"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879628","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative pharmacokinetics of fluoride secretion by major and labial minor salivary glands after oral fluoride intake","authors":"Ligiane Campos Bellose,&nbsp;Leonardo Libardi Pagotto,&nbsp;Deborah Rackel Caldas-da-Rocha,&nbsp;Cinthia Pereira Machado Tabchoury,&nbsp;Antônio Pedro Ricomini-Filho,&nbsp;Jaime Aparecido Cury","doi":"10.1016/j.archoralbio.2025.106488","DOIUrl":"10.1016/j.archoralbio.2025.106488","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate whether fluoride secretion by labial minor salivary glands exhibits a pharmacokinetic pattern comparable to that of major salivary glands after oral fluoride intake.</div></div><div><h3>Design</h3><div>In a randomized, blind and crossover study, eight healthy adult participants ingested aqueous NaF solutions containing 5, 10, or 20 mg of fluoride after overnight fasting. Saliva samples were collected at baseline (time 0) and at 5, 15, 30, 60, and 120 min post-ingestion from four sources: labial minor glands (LG), parotid glands (PG), floor of the mouth (FS), and whole saliva (WS). LG saliva and FS were collected using pipette tips under gentle suction, while PG saliva was obtained using the Carlson-Crittenden device and WS was obtained by asking participants to expectorate directly into a 1.5 mL microtube. Fluoride concentrations were determined using a validated micro-method with an inverted fluoride ion-selective electrode. Pharmacokinetic parameters - maximum concentration (Cmax) and area under the curve (AUC) - were calculated. Data were analyzed by two-way ANOVA and linear regression (α=0.05).</div></div><div><h3>Results</h3><div>Fluoride secretion from LG demonstrated a dose-dependent response, with Cmax values of 0.37 ± 0.04, 0.45 ± 0.04, and 0.76 ± 0.19 µg/mL, and AUC values of 29.0 ± 6.2, 33.6 ± 3.4, and 52.7 ± 11.5 µg F/mL <strong>× </strong> min for 5, 10, and 20 mg doses, respectively. These parameters did not differ significantly from those obtained from other salivary glands. A significant linear correlation was observed between the ingested fluoride dose and LG fluoride secretion (R² = 0.6469, p &lt; 0.0001).</div></div><div><h3>Conclusion</h3><div>The pharmacokinetic profile of fluoride secretion in labial minor salivary glands is quantitatively comparable to that of major salivary glands. Their use as an alternative source in fluoride pharmacokinetic studies appears valid, offering a non-invasive and practical sampling method.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"183 ","pages":"Article 106488"},"PeriodicalIF":2.1,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145879617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}