Archives of oral biology最新文献

{"title":"Bone marrow derived mesenchymal stem cells restored GLUT1 expression in the submandibular salivary glands of ovariectomized rats","authors":"Menna Abdulqader El-Badawy ,&nbsp;Mohamed Badawy ,&nbsp;Maha El Shahawy","doi":"10.1016/j.archoralbio.2024.106048","DOIUrl":"10.1016/j.archoralbio.2024.106048","url":null,"abstract":"<div><h3>Objective</h3><p>Loss of ovarian function in menopause is commonly associated with salivary gland dysfunction. The aim is to study the possible therapeutic effect of bone marrow mesenchymal stem cells (BM-MSCs) on the altered structure of the submandibular salivary glands (SMGs) of ovariectomized rats.</p></div><div><h3>Design</h3><p>Twenty-four female, adult, Wistar rats were used and distributed into three groups (8 rats/group). The control group included sham-operated rats. The ovariectomized group consisted of rats with removed ovaries. The third group consisted of ovariectomized rats received injections, via tail, of MSCs extracted from bone marrow of 3-weeks-old rat hind limb (BM-MSC group). Four weeks after BM-MSC transplantation, the bone mineral density (BMD) of the femur was detected. The SMG was dissected and processed for histological, immunohistochemical, and histomorphometric analyses.</p></div><div><h3>Results</h3><p>The ovariectomized rats depicted low BMD in the femur. The SMG acini revealed atrophy. The ductal and acinar cells depicted vacuolization and abnormal nuclear histology. GLUT1 immunostaining was decreased in SMG ducts. The BM-MSC group resumed the normal SMG histology and GLUT1 immunolabelling.</p></div><div><h3>Conclusions</h3><p>BM-MSC therapy restored the normal SMG structure and GLUT1 immunostaining in the treated ovariectomized rats, suggesting improved glucose transporting function.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the bioelectrical activity of the masticatory muscles in patients with narrowed maxillary transverse dimension compared to the occlusal norm","authors":"","doi":"10.1016/j.archoralbio.2024.106049","DOIUrl":"10.1016/j.archoralbio.2024.106049","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of the study was to determine how the electrical activity of the temporalis, masseter and sternocleidomastoid muscles differs in children with reduced transverse jaw dimension compared to children with normal occlusion. Design: It was a experimental study. Thirty-seven patients were included in the study. 18 in the study group received orthodontic treatment with removable appliances and 19 subjects were classified as normal occlusion subjects in the control group. A panoramic X-ray and digital intraoral scan were taken, followed by an surface electromyography of three muscle pairs (temporalis muscles, masseter muscles, sternocleidomastoid muscles) in resting position, while clenching and clenching on cotton rollers. Results: There was significantly greater activity in the experimental group than in the control group comparing muscles: temporalis muscles and masseter muscles in the resting position. Additionally, significantly greater activity of muscles in the control group was found during clenching. However, the asymmetry index of muscles indicates that there is significantly greater asymmetry of muscles activity in the experimental group. Compared to children with normal occlusion, children with a narrowed transverse dimension of the jaw have statistically significant differences in the bioelectrical activity of the temporalis, masseter and sternocleidomastoid muscles, as well as greater asymmetry in the bioelectrical voltage of the masseter muscles. Conclusions: Patients with reduced transverse dimension of the jaw are characterized by increased resting activity of the masticatory muscles and reduced functional activity of the masticatory muscles. These patients have increased asymmetry in the bioelectrical tension of the masticatory muscles.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141700505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Naltrexone accelerated oral traumatic ulcer healing and downregulated TLR-4/NF-kB pathway in Wistar rats","authors":"","doi":"10.1016/j.archoralbio.2024.106047","DOIUrl":"10.1016/j.archoralbio.2024.106047","url":null,"abstract":"<div><h3>Objective</h3><p>To assess the effect of naltrexone on oral mucosal healing using a traumatic ulcer model</p></div><div><h3>Design</h3><p>Wistar rats (n = 112) received distilled water (control) or naltrexone (0.5, 10, or 50 mg/kg/day). Ulcers were induced on the buccal mucosa using a round skin biopsy punch (diameter 6 mm). Euthanasia was performed on days 1, 3, 7, and 14. Healing was assessed by ulcer area, histological scores, histomorphometric analysis (number of polymorphonuclears, mononuclears, and fibroblasts), and collagen percentage. Immunohistochemistry for TLR-2, TLR-4, NF-kB, and CD31 was evaluated. Nociceptive threshold was measured daily.</p></div><div><h3>Results</h3><p>The 50 mg/kg group showed reduced ulcer area on days 1 (p &lt; 0.001), 3 (p &lt; 0.05), and 14 (p &lt; 0.01). In this group, there was, on day 14, an increase in the percentage of reepithelization (p = 0.043) and collagen (p &lt; 0.05), an increase in connective tissue maturation (p = 0.016), and on day 7 an increase in fibroblasts (p &lt; 0.001). The 10 mg/kg dose reduced the ulcer area on day 1 (p &lt; 0.001). The 50 mg/kg group showed lower expression of TLR-4 (p &lt; 0.001) on day 1, NF-kB on days 1 (p &lt; 0.05) and 14 (p &lt; 0.05), and CD31 on day 14 (p &lt; 0.05). The 0.5 and 10 mg/kg doses reduced TLR-4 expression on day 1 (p &lt; 0.05; p &lt; 0.01, respectively). Nociceptive threshold increased in the 50 mg/kg group (p &lt; 0.01).</p></div><div><h3>Conclusion</h3><p>Naltrexone enhanced traumatic oral ulcer healing by reducing TLR-4/NF-kB signaling and promoting fibroblast proliferation and collagen deposition. Additionally, naltrexone reduced pain in rats.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141637560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brusatol improves the efficacy of an anti-mouse-PD-1 antibody via inhibiting programmed cell death 1 ligand 1 expression in a murine head and neck squamous cell carcinoma model","authors":"Yanlin Wu ,&nbsp;Guilian Zhang ,&nbsp;Panpan Yin ,&nbsp;Jinlin Wen ,&nbsp;Ying Su ,&nbsp;Xinyan Zhang","doi":"10.1016/j.archoralbio.2024.106043","DOIUrl":"10.1016/j.archoralbio.2024.106043","url":null,"abstract":"<div><h3>Objective</h3><p>Combing PD-1/PD-L1 immune checkpoint inhibitors with natural products has exhibited better efficacy than monotherapy. Hence, the purpose of this research was to examine the anti-cancer effects of brusatol, a natural quassinoid-terpenoid derived from <em>Brucea javanica</em>, when used in conjunction with an anti-mouse-PD-1 antibody in a murine head and neck squamous cell carcinoma (HNSCC) model and elucidate underlying mechanisms.</p></div><div><h3>Design</h3><p>A murine HNSCC model and an SCC-15 cell xenograft nude mouse model were established to investigate the anti-cancer effects of brusatol and anti-PD-1 antibody. Mechanistic studies were performed using immunohistochemistry. Cell proliferation, migration, colony formation, and invasion were evaluated by MTT, migration, colony formation, and transwell invasion assays. PD-L1 levels in oral squamous cell carcinoma (OSCC) cells were assessed through qRT-PCR, flow cytometry, and western blotting assays. The impact of brusatol on Jurkat T cell function was assessed by an OSCC/Jurkat co-culture assay.</p></div><div><h3>Results</h3><p>Brusatol improved tumor suppression by anti-PD-1 antibody in HNSCC mouse models. Mechanistic studies revealed brusatol inhibited tumor cell growth and angiogenesis, induced apoptosis, increased T lymphocyte infiltration, and reduced PD-L1 expression in tumors. Furthermore, in vitro assays confirmed brusatol inhibited PD-L1 expression in OSCC cells and suppressed cell migration, colony formation, and invasion. Co-culture assays indicated that brusatol's PD-L1 inhibition enhanced Jurkat T cell-mediated OSCC cell death and reversed the inhibitory effect induced by OSCC cells.</p></div><div><h3>Conclusions</h3><p>Brusatol improves anti-PD-1 antibody efficacy by targeting PD-L1, suggesting its potential as an adjuvant in anti-PD-1 immunotherapy.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141539007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of disulfidptosis and periodontitis: New insights and clinical significance","authors":"Yixin Fan ,&nbsp;Wantong Liu ,&nbsp;Le Qi ,&nbsp;Qi Zhao ,&nbsp;Sining Li ,&nbsp;He Zou ,&nbsp;Chen Kong ,&nbsp;Zhiwei Li ,&nbsp;Jiwei Ren ,&nbsp;Zhihui Liu ,&nbsp;Bowei Wang","doi":"10.1016/j.archoralbio.2024.106046","DOIUrl":"10.1016/j.archoralbio.2024.106046","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aims to investigate and predict the therapeutic agents associated with disulfidptosis in periodontitis.</p></div><div><h3>Design</h3><p>The dataset GSE10334 was downloaded from the Gene Expression Omnibus (GEO) database and used to train a least absolute shrinkage and selection operator (LASSO) regression and support vector machine recursive feature elimination (SVM–RFE) algorithm to identify genes associated with disulfidptosis in periodontitis. GSE16134 validation sets, polymerase chain reaction (PCR), and gingival immunofluorescence were used to verify the results.Single-gene Gene Set Enrichment Analysis (GSEA) was performed to explore the potential mechanisms and functions of the characterized genes. Immune infiltration and correlation analyses were performed, and competing endogenous RNA (ceRNA) networks were constructed. Effective therapeutic drugs were then predicted using the DGIdb database, and molecular docking was used to validate binding affinity.</p></div><div><h3>Results</h3><p>Six genes (SLC7A11, SLC3A2, RPN1, NCKAP1, LRPPRC, and NDUFS1) associated with disulfidptosis in periodontitis were obtained. Validation results from external datasets and experiments were consistent with the screening results. Single-gene GSEA analysis was mainly enriched for antigen presentation and immune-related pathways and functions.Immune infiltration and correlation analyses revealed significant regulatory relationships between these genes and plasma cells, resting dendritic cell, and activated NK cells. The ceRNA network was visualized. And ME-344, NV-128, and RILUZOLE, which have good affinity to target genes, were identified as promising agents for the treatment of periodontitis.</p></div><div><h3>Conclusions</h3><p>SLC7A11, SLC3A2, RPN1, NCKAP1, LRPPRC, and NDUFS1 are targets associated with disulfidptosis in periodontitis, and ME-344, NV-128, and RILUZOLE are promising agents for the treatment of periodontitis.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141592414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of wound healing markers in gingival crevicular fluid following root-coverage procedures: A systematic review of randomized clinical trials","authors":"Vikender Singh Yadav ,&nbsp;Kanika Makker ,&nbsp;Nitesh Tewari ,&nbsp;Nitika Monga ,&nbsp;Rajiv Balachandran ,&nbsp;Ujjal Kumar Bhawal ,&nbsp;Ajay Mahajan","doi":"10.1016/j.archoralbio.2024.106035","DOIUrl":"10.1016/j.archoralbio.2024.106035","url":null,"abstract":"<div><h3>Objective</h3><p>Although several surgical techniques have been developed for treatment of gingival recession (GR), the underlying wound healing process remains relatively unexplored. This systematic review aimed to investigate the expression of wound healing markers in gingival crevicular fluid (GCF) before and after surgical treatment of GR.</p></div><div><h3>Design</h3><p>Randomized clinical trials (RCTs) reporting changes in the expression of GCF markers following any root coverage surgical procedure were identified from 4 electronic databases and manual searches followed by data extraction and result synthesis. The risk of bias (RoB) was assessed using Cochrane RoB 2.0 tool. Overall certainty of evidence was summarized using the Grading of Recommendations Assessment, Development and Evaluation (GRADE) tool.</p></div><div><h3>Results</h3><p>Four RCTs comprising 100 patients and investigating 15 biomarkers were included. Post-surgery, GCF levels of cytokines and inflammatory proteins were raised during the first 2–10 days of healing. MMP-8 levels increased during the first week followed by a gradual decline. RoB was found to be high for all studies and the overall certainty of evidence was very low.</p></div><div><h3>Conclusion</h3><p>A limited number of studies with large methodological variations precluded reliable conclusions. Well-designed studies powered for GCF markers’ levels that follow a standardized protocol for GCF sampling and processing are needed to draw conclusive evidence.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"M2 macrophage-derived exosomes for bone regeneration: A systematic review","authors":"Alireza Daneshvar ,&nbsp;Parisa Nemati ,&nbsp;Ali Azadi ,&nbsp;Reza Amid ,&nbsp;Mahdi Kadkhodazadeh","doi":"10.1016/j.archoralbio.2024.106034","DOIUrl":"10.1016/j.archoralbio.2024.106034","url":null,"abstract":"<div><h3>Objective</h3><p>This systematic review aims to evaluate existing evidence to investigate the therapeutic efficacy of M2 macrophage-derived exosomes in bone regeneration.</p></div><div><h3>Design</h3><p>A comprehensive search between 2020 and 2024 across PubMed, Web of Science, and Scopus was conducted using a defined search strategy to identify relevant studies regarding the following question: “What is the impact of M2 macrophage-derived exosomes on bone regeneration?”. Controlled in vitro and in vivo studies were included in this study. The SYRCLE tool was used to evaluate the risk of bias in the included animal studies.</p></div><div><h3>Results</h3><p>This review included 20 studies published. Seven studies were selected for only in vitro analysis, whereas 13 studies underwent both in vitro and in vivo analyses. The in vivo studies employed animal models, including 163 C57BL6 mice and 73 Sprague-Dawley rats. Exosomes derived from M2 macrophages were discovered to be efficacious in promoting bone regeneration and vascularization in animal models of bone defects. These effects were primarily confirmed through morphological and histological assessments. This remarkable outcome is attributed to the regulation of multiple signaling pathways, as evidenced by the findings of 11 studies investigating the involvement of miRNAs in this intricate process. In addition, in vitro studies observed positive effects on cell proliferation, migration, osteogenesis, and angiogenesis. Heterogeneity in study methods hinders direct comparison of results across studies.</p></div><div><h3>Conclusion</h3><p>M2 macrophage-derived exosomes demonstrate remarkable potential for promoting bone regeneration. Further research optimizing their application and elucidating the underlying mechanisms can pave the way for clinical translation.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The association between genetic factors and temporomandibular disorders: A systematic literature review","authors":"Ahid Amer Alshahrani ,&nbsp;Ravinder S. Saini ,&nbsp;Abdulmajeed Okshah ,&nbsp;Abdulkhaliq Ali F. Alshadidi ,&nbsp;Masroor Ahmed Kanji ,&nbsp;Rajesh Vyas ,&nbsp;Rayan Ibrahim H. Binduhayyim ,&nbsp;Naseer Ahmed ,&nbsp;Seyed Ali Mosaddad ,&nbsp;Artak Heboyan","doi":"10.1016/j.archoralbio.2024.106032","DOIUrl":"10.1016/j.archoralbio.2024.106032","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to investigate the correlation between genetic factors and the occurrence and progression of temporomandibular disorders (TMDs) using a comprehensive review and meta-analysis.</p></div><div><h3>Design</h3><p>A comprehensive search was conducted using the ScienceDirect, PubMed, Cochrane Library, Dimensions, and Emerald databases. A reviewer selected the study using modified PICO criteria, considering human subjects with TMDs, comparing different genetic factors among TMD and non-TMD patients, and reporting TMD signs and symptoms as outcomes. The methodological standards of the eligible papers were assessed using the Joanna Briggs Institute (JBI) Critical Appraisal Checklist for Non-randomized Experimental Investigations. Information was collected methodically and examined.</p></div><div><h3>Results</h3><p>The electronic database search yielded 851 articles, 19 of which were included in this study. The data analysis showed a significant influence of genetic factors, such as polymorphisms and gene differences, on the development of TMD signs and symptoms, such as myofascial pain, chronic pain, and disc displacement. In addition, gene polymorphism significantly influenced TMD development, with an odds ratio of 2.46 (1.93–3.14) and p of 0.00001.</p></div><div><h3>Conclusions</h3><p>Genetic factors significantly influenced TMD signs and symptoms, and genetic polymorphisms significantly influenced TMD onset and progression. Further research should be conducted in diverse settings with larger sample sizes to verify and validate these findings.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001535/pdfft?md5=1fc1e92f8fa457b6c78ba7f4a7a80356&pid=1-s2.0-S0003996924001535-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral behaviors and anxiety are significant predictors of jaw function limitation in patients with anterior disc displacement without reduction","authors":"Zhong-yi Fang ,&nbsp;Yang Yang ,&nbsp;Yuan Yao,&nbsp;Sha-sha Liu,&nbsp;Li-kun Liu,&nbsp;Shen-ji Lu,&nbsp;Hong Zeng,&nbsp;Bin Cai,&nbsp;Li-li Xu","doi":"10.1016/j.archoralbio.2024.106033","DOIUrl":"10.1016/j.archoralbio.2024.106033","url":null,"abstract":"<div><h3>Objective</h3><p>We aimed to describe jaw function characteristics in patients with anterior disc displacement without reduction (ADDWoR) using the jaw function limitation scale (JFLS), and to investigate the effects of biopsychosocial risk factors on limited jaw function.</p></div><div><h3>Design</h3><p>In this cross-sectional study of 636 patients with ADDWoR (females, 568; males, 68), we used the JFLS to assess jaw function. Behavioral, psychological, sociodemographic, and biomedical data were collected. Multivariate logistic regression analysis was used to determine risk factors affecting limited jaw function. A receiver operating characteristic curve was used to evaluate the predictive effect of these risk factors.</p></div><div><h3>Results</h3><p>ADDWoR-associated limitations included restricted jaw mobility and mastication, which exceeded median global functional limitations scale scores, especially mouth opening to bite an apple and chewing tough food. Females had greater limitations in jaw mobility, verbal and emotional communication, and overall. Multivariate logistic regression analysis findings indicated that oral behaviors, anxiety, sex, pain intensity, and maximal mouth opening (MMO) were predictive of limited jaw function (area under the curve, 72 %).</p></div><div><h3>Conclusion</h3><p>Patients with ADDWoR reported mastication and jaw mobility restrictions, with females having more pronounced limitations, and specific risk factors identified as significant predictors of jaw function limitations. Along with pain relief and improvement in MMO, appropriate psychological counseling and oral behavioral correction facilitates recovery of jaw function in such patients.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141581811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acquired pellicle and biofilm engineering with CaneCPI-5: Insights from proteomic and microbiomics analysis","authors":"Tamara Teodoro Araujo ,&nbsp;Aline Dionizio ,&nbsp;Thamyris de Souza Carvalho ,&nbsp;Ana Luiza Bogaz Debortolli ,&nbsp;Mariele Vertuan ,&nbsp;Beatriz Martines de Souza ,&nbsp;João Victor Frazão Camara ,&nbsp;Flavio Henrique-Silva ,&nbsp;Marcos Chiaratti ,&nbsp;Angélica Santos ,&nbsp;Lindomar Alves ,&nbsp;Milene Ferro ,&nbsp;Ana Carolina Magalhães ,&nbsp;Marília Afonso Rabelo Buzalaf","doi":"10.1016/j.archoralbio.2024.106025","DOIUrl":"10.1016/j.archoralbio.2024.106025","url":null,"abstract":"<div><h3>Objective</h3><p>In this <em>in vivo</em> proof-of-concept study, acquired pellicle engineering was implemented to promote alterations in the protein composition of the acquired enamel pellicle (AEP) and the bacterial composition of the dental biofilm after treatment with Sugarcane cystatin (CaneCPI-5).</p></div><div><h3>Design</h3><p>After prophylaxis, 10 volunteers rinsed (10 mL, 1 min) with the following solutions: 1) deionized water (H₂O- negative control or 2) 0.1 mg/mL CaneCPI-5. The AEP and biofilm were formed along 2 or 3 h, respectively. The AEP was collected with electrode filter papers soaked in 3 % citric acid. After protein extraction, samples were analyzed by quantitative shotgun label-free proteomics. The biofilm microbiome was collected with a dental curette. The DNA was extracted, amplified, and analyzed by 16S-rRNA Next Generation Sequencing (NGS).</p></div><div><h3>Results</h3><p>Treatment with CaneCPI-5 increased several proteins with antimicrobial, acid-resistance, affinity for hydroxyapatite, structural and calcium binding properties, such as Cysteine-rich-3 (6-fold-p = 0.03), Cystatin-B (5.5-fold-p &lt; 0.01), Neutrophil-defensin 1 (4.7-fold-p &lt; 0.01), Mucin (3.9-fold-p &lt; 0.01), Immunoglobulin-heavy-constant (3.8-fold-p &lt; 0.01) and Lactotransferrin (2.8-fold-p &lt; 0.01). Microbiome revealed that several commensal bacteria had their abundance increased after rinsing with CaneCPI-5, such as Corynebacterium and Neisseria, while Streptococcus and <em>Prevotella nigrescens</em> were decreased. The results indicate the efficiency of CaneCPI-5 in promoting beneficial changes in the AEP and biofilm, making this phytocystatin a potential target for incorporation into dental products.</p></div><div><h3>Conclusion</h3><p>Cane demonstrated the capability to alter the protein composition of the acquired enamel pellicle (AEP) and the initial colonizers of the biofilm, enhancing the presence of proteins and bacteria crucial for dental protection.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141473361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}