Archives of oral biology最新文献

{"title":"MicroRNA-155 rs767649 polymorphism is associated with susceptibility to peri-implantitis","authors":"Hao Fu ,&nbsp;Xiangrong Li ,&nbsp;Zeneng Xie ,&nbsp;Yinhua Jiang ,&nbsp;Huiling Qin","doi":"10.1016/j.archoralbio.2025.106219","DOIUrl":"10.1016/j.archoralbio.2025.106219","url":null,"abstract":"<div><h3>Objective</h3><div>To examine how the miR-155 rs767649 polymorphism affects miR-155 expression and to investigate its association with peri-implantitis susceptibility.</div></div><div><h3>Design</h3><div>One hundred and eighty-seven peri-implantitis patients and 196 healthy implant subjects were enrolled. The expression level of miR-155 in the subjects' serum was tested using qRT-PCR. The rs767649 polymorphism was genotyped with the TaqMan SNP genotyping assay. The genotype and allele distributions of miR-155 rs767649 polymorphism were compared between the case and control groups. Target genes of miR-155 were predicted using four online databases (ENCORI, miRDB, miRWalk and TargetScan). Functions and pathways of these target genes were annotated through GO and KEGG enrichment.</div></div><div><h3>Results</h3><div>The qRT-PCR results revealed that miR-155 was upregulated in patients with peri-implantitis (<em>P</em> &lt; 0.001) based on Student’s t test analysis. Carriers of the rs767649 TT genotype had higher levels of miR-155 expression in their serum (<em>P</em> &lt; 0.001) and a greater risk of peri-implantitis compared to those with the TA/AA genotype according to the chi-square test results (<em>P</em> = 0.016). The downstream target genes of miR-155 may be involved in pathways such as Kaposi sarcoma-associated herpesvirus infection, ubiquitin-protein hydrolysis, and cell differentiation.</div></div><div><h3>Conclusion</h3><div>The results revealed a correlation between the miR-155 rs767649 polymorphism and susceptibility to peri-implantitis. The rs767649 TT genotype upregulates miR-155 expression, which regulates downstream signaling pathways and increases the chance of peri-implantitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106219"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological evaluation of periapical lesions in developing molars of young male rats","authors":"Mariana Bena Gelio ,&nbsp;Paula Aparecida Nakazone-Guimarães ,&nbsp;Tiago Silva da Fonseca ,&nbsp;Evelin Carine Alves Silva ,&nbsp;Estela Sasso-Cerri ,&nbsp;Paulo Sérgio Cerri","doi":"10.1016/j.archoralbio.2025.106222","DOIUrl":"10.1016/j.archoralbio.2025.106222","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate the histopathological features of induced periapical lesions (PL) exposed to the oral environment at different time points in developing molars of rats.</div></div><div><h3>Methods</h3><div>Twenty-four 30-day-old Holtzman rats had the pulp chamber of the mandibular left first molar opened and root canals were exposed to the oral environment. According to the exposure time, the animals were distributed into three groups (n = 8 rats/group): PL induced for 3 days (PLG-3d), PL induced for 1week (PLG-1w) and PL induced for 9 weeks (PLG-9w). The healthy right first molars were used as control group (CG). In paraffin-embedded jaw sections, the number of inflammatory cells (ICs), IL-6-immunolabelled cells, osteoclasts and PLs area were measured. Data were subjected to two-way ANOVA analysis of variance and Tukey's post-test (p &lt; 0.05).</div></div><div><h3>Results</h3><div>The highest values of ICs and IL-6-immunolabelled cells were found in PLG-1w while no significant difference was observed between PLG-3d and PLG-9w specimens. PLG-1w showed a significantly greater number of osteoclasts than PLG-9w, but no significant difference was found in the PL area between these groups.</div></div><div><h3>Conclusions</h3><div>Although the peak of the inflammatory infiltrate, bone resorption and PL size were reached after 1-week, periapical lesions were established after 3 days in developing molars of rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106222"},"PeriodicalIF":2.2,"publicationDate":"2025-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Botulinum toxin type A inhibits hyperalgesia in the rat masseter muscle in a carrageenan model of myofascial pain","authors":"Ilana Dantas Neves ,&nbsp;Pedro Paulo Pereira Pinho ,&nbsp;Luciana Lyra Casais-e-Silva ,&nbsp;Marcio Cajazeira Aguiar","doi":"10.1016/j.archoralbio.2025.106218","DOIUrl":"10.1016/j.archoralbio.2025.106218","url":null,"abstract":"<div><h3>Objective</h3><div>Previous studies have shown that botulinum toxin type A (BoNT-A) attenuates nociception, but the underlying mechanisms remain unclear. Studies of experimental pain in humans have also shown conflicting results. Carrageenan is commonly used to produce short-term acute inflammation and hyperalgesia in animal models, and the effect of BoNT-A on carrageenan-induced pain in the masseter muscle has not been studied. This study evaluated the antinociceptive and anti-inflammatory effects of intramuscular injection of BoNT-A in an experimental model of inflammatory pain in the masseter muscle of rats.</div></div><div><h3>Design</h3><div>Carrageenan (2 %) was injected into the masseters of sixty rats pretreated with three sessions of BoNT-A (3.5 U/kg) or daily with ibuprofen (40 mg/kg) for seven days. Masseter injected with saline was used as a control. An electronic von Frey anesthesiometer determined the head withdrawal threshold before carrageenan and at 5 h, 1, 3, and 7 days following administration. The masseters were processed for paraffin embedding and H&amp;E staining and subjected to histomorphometric analysis 1 and 8 days after carrageenan administration.</div></div><div><h3>Results</h3><div>Pretreatments with BoNT-A or ibuprofen significantly decreased carrageenan-induced hyperalgesia. BoNT-A did not inhibit inflammation and tissue damage induced by carrageenan.</div></div><div><h3>Conclusions</h3><div>These findings reveal that BoNT-A promotes antinociceptive effects in the masseter muscle during painful conditions independently of anti-inflammatory mechanisms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106218"},"PeriodicalIF":2.2,"publicationDate":"2025-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Change and significance of connexin 43 in saliva extracellular vesicles from oral lichen planus patients","authors":"Chun Yao ,&nbsp;Haiyan Xin ,&nbsp;Si Liu ,&nbsp;Hong Wang ,&nbsp;Yanhong Ma ,&nbsp;Chao Yao ,&nbsp;Jian Meng","doi":"10.1016/j.archoralbio.2025.106217","DOIUrl":"10.1016/j.archoralbio.2025.106217","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to explore whether Connexin 43 (Cx43) in salivary extracellular vesicles (EVs) can serve as a biomarker for diagnosing oral lichen planus (OLP), an inflammatory oral mucosal disorder.</div></div><div><h3>Design</h3><div>The study assessed disease activity in OLP patients using the reticulum-erosion-ulcer disease activity score. Saliva EVs were isolated and purified with an EV Enrichment Kit. Transmission electron microscopy was used to examine the morphology and size of EVs, while Western blotting verified EV biomarkers. ELISA measured serum inflammatory factors, such as TNF-α and IL-17. The importance of Cx43 in the diagnosis of OLP was evaluated using receiver operating characteristic (ROC) curve analysis, and correlations were determined through Pearson analysis.</div></div><div><h3>Results</h3><div>The average diameter of isolated saliva EVs was approximately 110 nm, and they expressed high levels of known EV biomarkers. In OLP patients, both Cx43 mRNA and protein levels were significantly higher compared to healthy controls. Furthermore, Cx43 mRNA and protein levels increased gradually with increased disease severity, from the reticular type to the more severe erosion type of OLP. Cx43 mRNA and protein levels were found to effectively diagnosing OLP and correlated significantly with disease activity scores. Moreover, elevated Cx43 mRNA and protein levels showed strong positive correlations with serum TNF-α and IL-17 levels in OLP patients.</div></div><div><h3>Conclusion</h3><div>Cx43 mRNA and protein levels in salivary EVs serve as effective biomarkers for diagnosing OLP and are significantly associated with disease activity and inflammatory markers. This makes Cx43 a promising candidate for non-invasive diagnosis of OLP.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106217"},"PeriodicalIF":2.2,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580426","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatially resolved composition of bovine dental enamel in permanent incisors","authors":"Nathalie Murielly Rolim de Abreu ,&nbsp;Rudyard dos Santos Oliveira ,&nbsp;Frederico Barbosa de Sousa","doi":"10.1016/j.archoralbio.2025.106215","DOIUrl":"10.1016/j.archoralbio.2025.106215","url":null,"abstract":"<div><h3>Objective</h3><div>To quantify component volumes (mineral, organic, total water, firmly and loosely bound water volumes, and permeability) at histological points in bovine enamel and investigate their association with bovine enamel’s optical behavior and fracture toughness (FT).</div></div><div><h3>Design</h3><div>In vitro, cross-sectional, and experimental study. Longitudinal ground sections of ten permanent bovine incisors were analyzed using microradiography, polarizing microscopy, microhardness tester, and confocal laser fluorescence microscopy (CLFM). Continuous data were analyzed using regression and correlation tests.</div></div><div><h3>Results</h3><div>Bovine enamel exhibited gradients in both composition and FT from the surface inward, with the inner third showing positive birefringence in water immersion and opaque areas under immersion in air (explained by composition gradients), along with intermediate FT. Bovine enamel presented mineral volume of 79.6 % ( ± 2.7 %), water volume of 11.4 % ( ± 20.4 %), organic volume of 9.1 % ( ± 2.4 %), and permeability of 6.5 % ( ± 0.5 %). Water loss upon air drying was 1/5th of that in human enamel, representing a novel finding in enamel biology. The organic gradient was confirmed by CLFM. The ratio of firmly bound water to organic content was negatively associated with fracture toughness across the enamel layer.</div></div><div><h3>Conclusion</h3><div>bovine enamel differs from human enamel in terms of composition, birefringence, and permeability, requiring caution when interpreting results from studies using it to test dental products intended for humans. The composition of bovine enamel fully explained its optical behavior and partially explained its FT gradient.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106215"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143580427","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impaction risk increases significantly with each year of late third molar development in Western Canadian youths","authors":"Denver F. Marchiori ,&nbsp;Garnet V. Packota ,&nbsp;Prosanta Mondal ,&nbsp;Julia C. Boughner","doi":"10.1016/j.archoralbio.2025.106214","DOIUrl":"10.1016/j.archoralbio.2025.106214","url":null,"abstract":"<div><h3>Objective</h3><div>Third molar (M3) impaction affects millions of patients annually worldwide yet its etiology is unclear. Here we study an underexplored risk factor for impaction, later versus earlier M3 development, towards calculating impaction risk as a resource for clinicians.</div></div><div><h3>Design</h3><div>A total of 324 M3 areas (157 maxillary, 167 mandibular) were studied using retrospective CBCT scans and orthopantomograms of 99 Western Canadians (57 females, 42 males) aged 6–24 years. M3 crypt, crown and root development stage data were collected at an earlier timepoint T1 and then tested for association with M3 eruption status data documented at a later timepoint T2. This testing used Generalized Linear Mixed and Estimation modeling to explore relationships between M3 development stage and eruption status, and measure likelihood of versus functional emergence versus impaction risk (alpha level=0.05).</div></div><div><h3>Results</h3><div>Statistically, each year of late M3 development at T1 nearly doubled an M3’s risk of impaction at T2. Early-developing M3s had minimal impaction risk, as low as 5 %. This outcome was equivalent in upper and lower jaws and both sexes. Impacted M3s’ development was delayed due to later developmental onset, not to slower developmental rate.</div></div><div><h3>Conclusions</h3><div>Late-developing M3s are statistically more likely to become impacted. Clinical monitoring of M3 development from preadolescence is suggested. As early-developing M3s present minimal impaction risk, they are less likely to require prophylactic removal. This work provides foundational new insights into molar odontogenesis alongside practical information to help develop guidelines for managing patients with developing M3s.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106214"},"PeriodicalIF":2.2,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143600516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orofacial pain symptoms in sleep bruxer or non-sleep bruxer: Insights from a population-based survey of puerperal women","authors":"Thiago Azario de Holanda ,&nbsp;Luana Patrícia Marmitt ,&nbsp;Juraci Almeida Cesar ,&nbsp;Noéli Boscato","doi":"10.1016/j.archoralbio.2025.106205","DOIUrl":"10.1016/j.archoralbio.2025.106205","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the prevalence of orofacial pain (OP) symptoms among a large sample of women who self-identified as sleep bruxers or non-sleep bruxers and who had given birth in 2019 in the municipality of Rio Grande, RS, Southern Brazil.</div></div><div><h3>Methods</h3><div>A standardized self-questionnaire was administered to assess OP symptoms. Chi-square tests were employed to compare proportions, and multivariate Poisson regression analysis with robust variance adjustment was used to estimate associations with sleep bruxers and non-sleep bruxers.</div></div><div><h3>Results</h3><div>A total of 2225 women were included in the study, with 78 (3.5 %) reporting OP symptoms. In the adjusted analysis, self-reported OP symptoms were significantly associated with non-sleep bruxer women living with three or more people in the household (PR=8.40; 95 % CI 1.85–38.11), as well as severe anxiety (PR= 4.73; 95 % CI 2.00–11.18). Among sleep bruxers, a significantly lower OP symptoms prevalence for those who were able to rely on their friends in times of distress (PR= 0.17; 95 % CI 0.10–0.30), while those with personality disorders exhibited significantly higher OP symptoms prevalence (PR= 3.30; 95 % CI 1.04–10.45).</div></div><div><h3>Conclusions</h3><div>The prevalence of OP symptoms was higher among non-sleep bruxer women living with three or more individuals in the household and among those experiencing severe anxiety. Indeed, sleep bruxer women with personality disorders had a higher prevalence of OP symptoms, while those with strong social support demonstrated a lower prevalence.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106205"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole-genome sequencing analysis of Cutibacterium spp. recovered from health care-associated endodontic infections","authors":"Mónica Álvarez-Muñoz ,&nbsp;Christian Jerez-Olate ,&nbsp;Andrés Opazo-Capurro ,&nbsp;Raúl Alcántara-Dufeu ,&nbsp;Helia Bello-Toledo ,&nbsp;Gerardo González-Rocha ,&nbsp;Gabriela Sánchez-Sanhueza","doi":"10.1016/j.archoralbio.2025.106207","DOIUrl":"10.1016/j.archoralbio.2025.106207","url":null,"abstract":"<div><h3>Objective</h3><div><em>Cutibacterium acnes</em> (<em>C. acnes</em>) has been detected in culture and molecular studies of endodontic microbiota, indicating its potential as a neglected pathogen in endodontic infections. This study aimed to conduct a complete genomic analysis of three <em>Cutibacterium</em> spp. isolates obtained from persistent endodontic infections in patients from Chile.</div></div><div><h3>Design</h3><div>Whole genome sequencing and comprehensive genomic analysis using diverse bioinformatics platforms and databases were carried out.</div></div><div><h3>Results</h3><div><em>In-silico</em> analysis confirmed that strains A1 and E3 correspond to <em>C. acnes</em> species, while strain B1 corresponds to <em>C. namnetense</em>. Additionally, <em>C. acnes</em> strain A1 belongs to the phylotype IA₁, sequence type (ST) 4 and belongs to the clonal complex (CC) 4, whereas <em>C. acnes</em> strain E3 belongs to a novel ST. No antibiotic resistance, however, a variable distribution and identity of the VF was found.</div></div><div><h3>Conclusions</h3><div>Detailed analysis of genes associated with virulence factors shed light on the intrinsic pathogenic capacity of these strains, which may indicate differential adaptation to their specific niches or diversity in pathogenicity within the species.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106207"},"PeriodicalIF":2.2,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143487783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"STING regulates porphyromonas gingivalis lipopolysaccharide-induced pyroptosis and inflammatory response through the NF-κB/NLRP3 signaling pathway in human gingival fibroblasts","authors":"Bo Kou ,&nbsp;Yuna Zhang ,&nbsp;Wei Zhang,&nbsp;Jifang Zhang,&nbsp;Riwen Jiang","doi":"10.1016/j.archoralbio.2025.106197","DOIUrl":"10.1016/j.archoralbio.2025.106197","url":null,"abstract":"<div><h3>Objective</h3><div>The production of reactive oxygen species caused by antimicrobial response during periodontitis leads to the activation of NOD-like receptor protein 3 (NLRP3) inflammasome and pyroptosis. Stimulator of interferon genes (STING) has been found to be involved in regulating pyroptosis and inflammation in a variety of diseases. The present study aimed to investigate whether STING is involved in Porphyromonas gingivalis lipopolysaccharide (P.g LPS)-stimulated human gingival fibroblasts (HGFs) by regulating pyroptosis and inflammation.</div></div><div><h3>Design</h3><div>After culturing and identifying HGFs, HGFs were treated with P.g LPS. Constructs of si-STING were transfected into HGFs, which were then stimulated with P.g LPS for 24 h. Subsequently, cell viability, pyroptosis, inflammation, oxidative stress and alterations in the STING/TANK-binding kinase 1 (TBK1)/interferon regulatory factor 3 (IRF3)/nuclear factor-kappaB (NF-κB)/NLRP3 signalling pathway were detected.</div></div><div><h3>Results</h3><div>P.g LPS significantly enhanced STING expression in HGFs. Downregulation of STING rescued P.g LPS-enhanced pyroptosis, oxidative stress and inflammation in HGFs. Moreover, STING was found to bind directly to TBK1 to increase IRF3 phosphorylation and nuclear translocation of NF-κB, thus promoting NLRP3 inflammasome activation. Downregulation of STING rescued P.g LPS-enhanced TBK1/IRF3/NF-κB/NLRP3 pathway activation.</div></div><div><h3>Conclusion</h3><div>STING/TBK1/IRF3/NF-κB/NLRP3 is a key pathway governing pyroptosis, oxidative stress and inflammation of HGFs induced by LPS.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106197"},"PeriodicalIF":2.2,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143510964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Systemic administration of polyphenols from dealcoholized red wine reduces inflammation and bone resorption in established apical periodontitis in male rats","authors":"Romulo de Oliveira Sales-Junior ,&nbsp;Bharbara de Moura Pereira ,&nbsp;Rafaela Ricci ,&nbsp;Nathália Evelyn da Silva Machado ,&nbsp;Julissa Denisse Arguello Alvarado ,&nbsp;Ana Beatriz Carreto ,&nbsp;Edilson Ervolino ,&nbsp;Anil Kishen ,&nbsp;Luciano Tavares Ângelo Cintra ,&nbsp;João Eduardo Gomes-Filho","doi":"10.1016/j.archoralbio.2025.106206","DOIUrl":"10.1016/j.archoralbio.2025.106206","url":null,"abstract":"<div><h3>Objective</h3><div>To analyze the effect of supplementation with dealcoholized red wine on the severity of apical periodontitis after it had been established.</div></div><div><h3>Design</h3><div>Thirty-two male Wistar rats were arranged into four groups: water as control, dealcoholized red wine, red wine, and alcohol. Apical periodontitis was induced by pulp exposure for 30 days when supplementation started and continued daily for 30 days. After the supplementation period, the rats were euthanized, and maxillae and jaws were removed to evaluate the inflammatory response and the bone resorption through microtomographic, histological and immunohistochemical analysis. Statistical tests were applied at 5 % significance.</div></div><div><h3>Results</h3><div>The dealcoholized red wine group demonstrated a significant reduction in bone resorption volume and increase in bone volume fraction and trabecular parameters (p &lt; 0.05). The dealcoholized red wine and red wine groups exhibited mild inflammation compared to the control and alcohol groups that showed moderate and severe inflammation respectively (p &lt; 0.05). The immunoreaction was significantly lower in the dealcoholized red wine group than in the control and alcohol groups for the TNF-α, IL-1β and TRAP cells (p &lt; 0.05). Furthermore, OPG in dealcoholized red wine was similar to red wine and control but higher than in the alcohol group (p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>Supplementation with dealcoholized red wine in rats decreased inflammation and bone loss in established apical periodontitis. This study highlights the potential of systemic administration of polyphenols from red wine in decreasing inflammation and bone resorption of established apical periodontitis in rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106206"},"PeriodicalIF":2.2,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143463994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}