Archives of oral biology最新文献_第4页

Raman confocal microscopy atlas of human tooth

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-08 DOI: 10.1016/j.archoralbio.2025.106189

P.-Y. Collart-Dutilleul , S. Piglionico , R. Younes , Y. Messat , S. Garrabé , H. Salehi , J.-C. Durand , F. Cuisinier , A. Desoutter

{"title":"Raman confocal microscopy atlas of human tooth","authors":"P.-Y. Collart-Dutilleul , S. Piglionico , R. Younes , Y. Messat , S. Garrabé , H. Salehi , J.-C. Durand , F. Cuisinier , A. Desoutter","doi":"10.1016/j.archoralbio.2025.106189","DOIUrl":"10.1016/j.archoralbio.2025.106189","url":null,"abstract":"<div><h3>Objective</h3><div>Dental histology is a field that has been studied since the early 19th century. Most of the techniques used have been based on white-light microscopy or histological staining, or more recently immunohistochemical staining. With the advent of lasers coupled to confocal microscopy, Raman spectra can be measured in large numbers to create a detailed chemical atlas of the human tooth, offering new insights into its composition and structure.</div></div><div><h3>Design</h3><div>A total of twenty teeth, with 5 teeth from each type premolar, molar, incisor and canine were selected. Five teeth of different types pre-molar and molar incisor canine (total of 20 teeth) These teeth were sectioned and polished, and pulps extracted and cut into thin layers, to perform chemical mapping of all these tissues and components, including secondary structures, based on Raman scattering.</div></div><div><h3>Results</h3><div>We obtained images reconstructed from the intensities of the various characteristic peaks, enabling us to create an atlas of the tooth. A part of the result confirm previous study, but some structures have been revealed for the first time by chemical cartography: cementum dentin junction, dental pulp, Retzius striae, scallop pattern, Hunter-Schreger bands, sheat enamel prism content and dentin branches.</div></div><div><h3>Conclusion</h3><div>The present study thus provides the dental research and practice community with a complete chemical mapping of the fundamental and secondary constituents of the dental organ, with optical resolution.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106189"},"PeriodicalIF":2.2,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143420201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The biocompatibility, penetrability, sealing ability, and antibacterial properties of iRoot SP compared to AH plus: An In Vitro evaluation

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-07 DOI: 10.1016/j.archoralbio.2025.106188

Yiling Li , Bolei Li , Siyue Lai , Xiao Guo , Yu Fan , Haohao Wang , Lei Cheng

{"title":"The biocompatibility, penetrability, sealing ability, and antibacterial properties of iRoot SP compared to AH plus: An In Vitro evaluation","authors":"Yiling Li , Bolei Li , Siyue Lai , Xiao Guo , Yu Fan , Haohao Wang , Lei Cheng","doi":"10.1016/j.archoralbio.2025.106188","DOIUrl":"10.1016/j.archoralbio.2025.106188","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the biocompatibility, penetrability, sealing ability, and antibacterial properties of iRoot SP compared to AH Plus in root canal filling.</div></div><div><h3>Design</h3><div>Two types of iRoot SP and AH Plus were used for <em>in vivo</em> evaluation. The solubility of sealers was tested according to the International Organization for Standardization (ISO). Cell Counting kit-8 was performed for biocompatibility analysis. To evaluate the sealing efficacy, 27 extracted human single-rooted teeth were treated with the single-cone obturation technique. The penetrability was analyzed using confocal laser scanning microscope and the sealing ability was assessed through micro-computed tomography. The biofilm of <em>Enterococcus faecalis</em> was formed on the coagulated root canal sealers to determine the antibacterial properties by colony-forming units and scanning electronic microscopy.</div></div><div><h3>Results</h3><div>All sealers exhibited standard solubility (<3 %). The biocompatibility of the three root canal sealers was excellent with no statistically significant differences compared to the control group (P > 0.05). The penetrability of both iRoot SP variants was greater than that of AH Plus, especially in the apical third of teeth (P < 0.001). No significant differences in sealing efficacy among the sealers were observed (P > 0.05). IRoot SP also reduced <em>Enterococcus faecalis</em> counts and inhibited the biofilm formation compared to AH Plus (P < 0.01).</div></div><div><h3>Conclusions</h3><div>IRoot SP exhibited good biocompatibility. It has superior penetrability and enhanced antibacterial properties compared to AH Plus, but shown no difference in sealing ability, warranting further <em>in vivo</em> studies and long-term assessments. The research underscores the strong potential of bioceramic sealers for clinical applications.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106188"},"PeriodicalIF":2.2,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143378795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular polysaccharide-rich biofilm reduces chlorhexidine effect on enamel demineralization: An in situ study

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-03 DOI: 10.1016/j.archoralbio.2025.106187

Leonardo Libardi Pagotto, Jaime Aparecido Cury, Antonio Pedro Ricomini-Filho

{"title":"Extracellular polysaccharide-rich biofilm reduces chlorhexidine effect on enamel demineralization: An in situ study","authors":"Leonardo Libardi Pagotto, Jaime Aparecido Cury, Antonio Pedro Ricomini-Filho","doi":"10.1016/j.archoralbio.2025.106187","DOIUrl":"10.1016/j.archoralbio.2025.106187","url":null,"abstract":"<div><h3>Objectives</h3><div>This study investigated the effect of chlorhexidine (CHX) on demineralization, cell viability and matrix composition in EPS-rich (EPS<sup>+</sup>) and poor (EPS<sup>-</sup>) oral biofilms.</div></div><div><h3>Design</h3><div>A split-mouth and crossover <em>in situ</em> study was conducted with six participants over two phases of 14 days each. During a lead-in step, biofilms were formed under either sucrose exposure (EPS<sup>+</sup>) or glucose + fructose exposure (EPS<sup>-</sup>). In the experimental step, participants rinsed with either 0.9 % NaCl (negative control) or 0.12 % CHX for seven days.</div></div><div><h3>Results</h3><div>Data showed that EPS<sup>+</sup> biofilms exhibited higher enamel surface hardness loss (%SHL), lesion area (∆S) and concentrations of soluble and insoluble EPS, compared to EPS<sup>-</sup> biofilms. After treatments, CHX significantly reduced the counts of colony-forming units (CFU) in both EPS<sup>-</sup> and EPS<sup>+</sup> biofilms. However, CHX only reduced ∆S within the EPS<sup>-</sup> group. There was no significant difference in demineralization change (%∆S) among treatments. Additionally, CHX decreased soluble and insoluble EPS concentrations in the EPS<sup>+</sup> group.</div></div><div><h3>Conclusion</h3><div>While CHX effectively reduced bacterial counts, its antimicrobial effect may not be enough to reduce the enamel demineralization caused by mature EPS-rich biofilms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"173 ","pages":"Article 106187"},"PeriodicalIF":2.2,"publicationDate":"2025-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multi-strain probiotic formula modulates expression of β-defensin-2, β-defensin-3, and TLR-4 in male rats with apical periodontitis 多菌种益生菌配方调节雄性根尖牙周炎大鼠β-防御素-2、β-防御素-3和TLR-4的表达。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106137

Leopoldo Cosme-Silva , Renan Dal-Fabbro , Fernanda de Lima Pontes , Leticia Cabrera Capalbo , Edilson Ervolino , Luciano Tavares Angelo Cintra , Juan José Segura-Egea , João Eduardo Gomes-Filho

{"title":"Multi-strain probiotic formula modulates expression of β-defensin-2, β-defensin-3, and TLR-4 in male rats with apical periodontitis","authors":"Leopoldo Cosme-Silva , Renan Dal-Fabbro , Fernanda de Lima Pontes , Leticia Cabrera Capalbo , Edilson Ervolino , Luciano Tavares Angelo Cintra , Juan José Segura-Egea , João Eduardo Gomes-Filho","doi":"10.1016/j.archoralbio.2024.106137","DOIUrl":"10.1016/j.archoralbio.2024.106137","url":null,"abstract":"<div><h3>Objective</h3><div>Evaluate whether a multi-strain probiotic formula affects blood parameters (hematologic, calcium, and phosphorus levels) and alters the expression of β-defensin-2, β-defensin-3, and toll-like receptor 4 in male rats with induced apical periodontitis (AP).</div></div><div><h3>Design</h3><div>Wistar rats were divided into two groups (n = 8 each): (1) rats with AP on a regular diet (Control) and (2) rats with AP on a regular diet supplemented with the multi-strain probiotic GNC Probiotic Complex (GCP) at one billion CFU. AP was induced by exposing the dental pulp of the first molars to the oral environment. GCP was administered daily via gavage for 30 days during AP development. After 30 days, animals were anesthetized, a cardiac puncture was performed, and 5 mL of blood was collected for hematologic, calcium, and phosphorus analysis. Animals were then euthanized, and mandibles were removed for histological and immunochemical analysis of β-defensin-2, β-defensin-3, and toll-like receptor 4. Statistical analyses used Mann–Whitney U and Student’s t-tests, with significance at P < 0.05.</div></div><div><h3>Results</h3><div>No significant differences were observed in blood parameters between the Control and GCP groups (P > 0.05). In AP, the Control group showed more intense inflammatory infiltrates and higher median severity scores than the GCP group (P < 0.05). Immunoreactivity levels for β-defensin-2, β-defensin-3, and toll-like receptor 4 were significantly increased in the GCP group (P < 0.05).</div></div><div><h3>Conclusion</h3><div>Probiotic complex reduces inflammation and enhances immunolabeling of β-defensin-2, β-defensin-3, and toll-like receptor 4 in AP.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106137"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells 截断重组Jagged1与人IgG1 Fc融合激活人牙周韧带细胞Notch靶基因。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106138

Y Nhu Tran , Ajjima Chansaenroj , Araya Jivaphetthai , Thanaphum Osathanon , Wanatchaporn Arunmanee

{"title":"Truncated recombinant Jagged1 fused with human IgG1 Fc activates Notch target genes in human periodontal ligament cells","authors":"Y Nhu Tran , Ajjima Chansaenroj , Araya Jivaphetthai , Thanaphum Osathanon , Wanatchaporn Arunmanee","doi":"10.1016/j.archoralbio.2024.106138","DOIUrl":"10.1016/j.archoralbio.2024.106138","url":null,"abstract":"<div><h3>Objective</h3><div>Jagged1, a Notch ligand, is essential for osteogenic differentiation in human periodontal ligament cells (hPDLs) by interacting with Notch2 to induce osteogenic markers, alkaline phosphatase activity, and mineral deposition. However, its large size hampers absorption and distribution of biomaterials. This study aimed to identify the critical region of Jagged1 necessary for its interaction with Notch2 to create a truncated version that retains osteogenic activity but with improved delivery characteristics.</div></div><div><h3>Methods</h3><div>Truncated versions of Jagged1 were designed by deleting C-terminal regions, focusing on the importance of the N-terminal domain. Both truncated and full-length Jagged1 were fused with human IgG1 Fc (Jagged1-Fc) and expressed in Chinese hamster ovary cells. hPDLs treated with these constructs were analyzed for Notch target gene expression using real-time PCR. Mineral deposition was assessed using alizarin red S staining.</div></div><div><h3>Results</h3><div>Both truncated and full-length Jagged1-Fc increased the expression of Notch target genes (<em>Hes1, Hey1</em>, and <em>ALP</em>) in hPDLs, indicating successful activation of Notch signaling. However, only the full-length Jagged1-Fc enhanced mineral deposition, while the truncated version did not.</div></div><div><h3>Conclusions</h3><div>Full-length Jagged1-Fc is required for mineral deposition and complete osteogenic differentiation in hPDLs. The truncated versions, while capable of activating Notch signaling, are ineffective in promoting mineralization, underscoring the importance of the entire protein for clinical applications in bone regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106138"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of microorganisms on bone homeostasis in apical periodontitis 微生物对根尖牙周炎患者骨稳态的影响。

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106153

Dan Pan , Yu Hao , Yuyan Tao , Bolei Li , Lei Cheng

{"title":"The influence of microorganisms on bone homeostasis in apical periodontitis","authors":"Dan Pan , Yu Hao , Yuyan Tao , Bolei Li , Lei Cheng","doi":"10.1016/j.archoralbio.2024.106153","DOIUrl":"10.1016/j.archoralbio.2024.106153","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to provide an overview of the role of microorganisms in the onset and progression of periapical diseases, particularly regarding their effects on bone homeostasis.</div></div><div><h3>Design</h3><div>The search for this narrative review was conducted in PubMed, Web of Science and Google Scholar using relevant keywords, including checking reference lists of journal articles by hand searching.</div></div><div><h3>Results</h3><div>Microorganisms directly promote osteoclasts through pathways such as nuclear factor-κB (NF-κB) and extracellular regulated protein kinases (ERK), while inhibiting osteoblasts function by interfering with the wingless-related integration site (Wnt)/β-catenin pathway in the periapical area. Moreover, microorganisms indirectly regulate periapical bone homeostasis by inducing programmed cell death and modulating the immune microenvironment through the activation of innate immunity via pattern-recognition receptors (PRRs) and subsequent cascades of responses. Among these microorganisms, <em>Enterococcus faecalis</em>, <em>Porphyromonas gingivalis</em> and <em>Fusobacterium nucleatum</em> play significant roles.</div></div><div><h3>Conclusion</h3><div>Microorganisms regulate pathways such as NF-ĸB and Wnt/β-catenin, as well as programmed cell death and the immune microenvironment in the periapical area, thereby disrupting bone homeostasis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106153"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2024.106149

Yong Liu , Jiaying Nie , Ying Huang , Yunyan Yang , Wenen Su , Yumei Zhang , Zhuoqiao Gao , Shaohui Deng , Meilin Li , Shaoyan Lian , Jieying Li , Chaoqun Liu

{"title":"m6A-related genes ALKBH5 and RBMX as prognostic and progression biomarkers in Chinese oral squamous cell carcinoma patients","authors":"Yong Liu , Jiaying Nie , Ying Huang , Yunyan Yang , Wenen Su , Yumei Zhang , Zhuoqiao Gao , Shaohui Deng , Meilin Li , Shaoyan Lian , Jieying Li , Chaoqun Liu","doi":"10.1016/j.archoralbio.2024.106149","DOIUrl":"10.1016/j.archoralbio.2024.106149","url":null,"abstract":"<div><h3>Objective</h3><div>N6-methyladenosine (m6A) RNA dysregulation is crucial for cancer development. The study aimed to explore the effects of m6A modification in oral squamous cell carcinoma (OSCC) and its potential as a biomarker and therapeutic target.</div></div><div><h3>Design</h3><div>We first analyzed m6A-related gene expression and its impact on OSCC prognosis and progression using the TCGA database. Subsequently, a Chinese cohort of 134 samples was used for validation. Bioinformatics analysis was conducted with TCGA data, and m6A levels were measured in the validation cohort using a quantification kit. Survival analysis was performed to study the relationship between m6A-related genes and OSCC prognosis in the Chinese population. The expression of m6A-related genes was assessed by using quantitative real-time PCR, Western blot analysis, and immunohistochemistry.</div></div><div><h3>Results</h3><div>In the TCGA database, we found dysregulated expressions of METTL14, ALKBH5, YTHDF2, HNRNPC, LRPPRC, HNRNPA2B1, IGF2BP2, and RBMX in OSCC. Based on this, we observed significantly elevated total m6A content in OSCC tissues compared to normal controls in the validation cohort. Among the m6A candidate genes, only ALKBH5 and RBMX upregulation were found to be independent prognostic risk factors for poor OSCC survival in the Chinese population. And the inclusion of these two genes had a higher area under the curve for 3-year (0.705, 0.826), and 5-year (0.715, 0.788) overall survival compared to the model that only considered clinical parameters.</div></div><div><h3>Conclusions</h3><div>We found the upregulation of m6A status in OSCC, of which, ALKBH5 and RBMX may serve as promising diagnostic and prognostic biomarkers for Chinese patients with OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106149"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro development of a radicular cyst: A morphological investigation of a spheroid cyst-like model associated with fibroblasts

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-02-01 DOI: 10.1016/j.archoralbio.2025.106186

D. Nascimento , LM Brand , L. Bernardi , JM Vasconcelos , IC Guedes , Figueiredo JAP , F. Visioli , ML Lamers , PV Rados

{"title":"In vitro development of a radicular cyst: A morphological investigation of a spheroid cyst-like model associated with fibroblasts","authors":"D. Nascimento , LM Brand , L. Bernardi , JM Vasconcelos , IC Guedes , Figueiredo JAP , F. Visioli , ML Lamers , PV Rados","doi":"10.1016/j.archoralbio.2025.106186","DOIUrl":"10.1016/j.archoralbio.2025.106186","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to improve the <em>in vitro</em> model of tooth radicular cyst previously developed by incorporating stromal components and to describe histologic analysis.</div></div><div><h3>Design</h3><div>A radicular cystogenesis-like 3D model was generated using HaCaT cells (1 × 10<sup>5</sup>) to developing spheroid. After 24 h, spheroids were embedded in non-polymerized collagen in combination with 1 × 10<sup>5</sup> fibroblast cells (HaCaT + 1 × 10<sup>5</sup> hFIB) to mimic stromal microenvironment. Micrographs were taken to evaluate the cystic stability and dispersion area, while histological hematoxylin/eosin staining was used to measure the ratio of epithelial lining area. Analysis was conducted on days 1, 3, and 7 using ImageJ software. Statistical analyses were performed using GraphPad Prism 5 software.</div></div><div><h3>Results</h3><div>The model, with fibroblasts included, preserved the cystic structure and allowed cyst growth, with an increase in both the area and dispersion of the cystic structure throughout the experimental period (p < 0.05). Histological analysis of the cyst model revealed morphological similarities with <em>in vivo</em> tooth radicular cyst biopsies, showing a cystic cavity lined by an epithelial layer, surrounded by collagen and fibroblasts. Additionally, the cavity area increased while the limiting epithelial area decreased. The highest epithelial area-to-total area ratio was observed in day 1 spheroids, while the lowest was found on day 7 (p < 0.05).</div></div><div><h3>Conclusion</h3><div>The incorporation of fibroblasts improved the <em>in vitro</em> cystogenesis model, since it did not interfere with the model’s development and more closely mimicked the <em>in vivo</em> microenvironment.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106186"},"PeriodicalIF":2.2,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143153944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phospho-cofilin predicts efficiency of Fasudil for oral squamous cell carcinoma treatment through Yes-associated protein inhibition

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-01-29 DOI: 10.1016/j.archoralbio.2025.106185

Ying Wu , Yuchen Jiang , Lanxin Jiang , Yang Peng , Tong Zhou , Xiaoqiang Xia , Feifei Hou , Qiuyun Yuan , Lu Ye , Weideng Wei , Jiuge Zhang , Qianming Chen , Xiaodong Feng

{"title":"Phospho-cofilin predicts efficiency of Fasudil for oral squamous cell carcinoma treatment through Yes-associated protein inhibition","authors":"Ying Wu , Yuchen Jiang , Lanxin Jiang , Yang Peng , Tong Zhou , Xiaoqiang Xia , Feifei Hou , Qiuyun Yuan , Lu Ye , Weideng Wei , Jiuge Zhang , Qianming Chen , Xiaodong Feng","doi":"10.1016/j.archoralbio.2025.106185","DOIUrl":"10.1016/j.archoralbio.2025.106185","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluates Fasudil, a Rho-associated coiled-coil-containing protein kinase (ROCK) inhibitor, for its potential to inhibit oral squamous cell carcinoma (OSCC) growth and explores phospho-cofilin as a potential biomarker for prediction treatment efficiency of Fasudil in OSCC.</div></div><div><h3>Design</h3><div>A cohort of 109 OSCC patients provided tissue samples for phospho-cofilin expression analysis and survival analysis. The study examined the effect of Fasudil on OSCC cell lines HSC-3, UM1, and CAL33, assessing tumor growth inhibition through various in vitro and in vivo experiments. ROCK inhibition response and downstream mechanisms were explored by RNA sequencing, q-PCR, and immunofluorescence.</div></div><div><h3>Results</h3><div>High phospho-cofilin expression in OSCC tissues correlated with poor patient outcomes and was a reliable biomarker for ROCK activity. Fasudil inhibited growth in OSCC cell lines, particularly those with high phospho-cofilin expression. ROCK inhibition led to downregulation of Yes-associated protein (YAP) activity, resulting in suppressed tumor proliferation and increased apoptosis both in vitro and in vivo.</div></div><div><h3>Conclusions</h3><div>Inhibition of ROCK/phospho-cofilin/YAP by Fasudil could suppress OSCC proliferation, while phospho-cofilin served as a potential biomarker of OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106185"},"PeriodicalIF":2.2,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of Hypoxia-Inducible Factor-1a stabilization on bone regeneration during distraction osteogenesis: A systematic review of animal studies

IF 2.2 4区医学

Archives of oral biology Pub Date : 2025-01-23 DOI: 10.1016/j.archoralbio.2025.106184

Shaghayegh Najary , Hanieh Nokhbatolfoghahaei , Arash Khojasteh

{"title":"The effect of Hypoxia-Inducible Factor-1a stabilization on bone regeneration during distraction osteogenesis: A systematic review of animal studies","authors":"Shaghayegh Najary , Hanieh Nokhbatolfoghahaei , Arash Khojasteh","doi":"10.1016/j.archoralbio.2025.106184","DOIUrl":"10.1016/j.archoralbio.2025.106184","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review described Hypoxia-Inducible Factor-1a stabilization or upregulation approaches along with underlying signaling pathways and assessed bone regeneration, angiogenesis, and consolidation time during DO in animal models.</div></div><div><h3>Design</h3><div>A comprehensive and systematic search of electronic databases including PubMed, Scopus, and ScienceDirect was performed till December 26, 2023. The search was limited to English articles, and no time restrictions were applied.</div></div><div><h3>Results</h3><div>A total of 14 studies met the inclusion criteria and were included for final review. Four methods have been shown to activate the HIF pathway including genetic, pharmacological, mechanical, and cell preconditioning approaches. Deferoxamine (DFO) was administered as a pharmacological hypoxia-mimicking agent in many studies reporting acceptable outcomes on bone regeneration and acceleration of bone consolation. Applying mechanical loads at the optimal rate and amplitude serves as a minimally invasive approach with acceptable results. HIF-related signaling pathways increase osteogenesis and angiogenesis during DO, potentially through VHL/HIF-1a/VEGF, Wnt/β-catenin, and Mesenchymal-Epithelial transition (MET) signaling pathways.</div></div><div><h3>Conclusion</h3><div>Activation of HIF-related signaling pathways enhances and accelerates bone regeneration during the consolidation phase of distraction osteogenesis. The most feasible approach with the least side effects must be selected for further clinical studies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"172 ","pages":"Article 106184"},"PeriodicalIF":2.2,"publicationDate":"2025-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143082617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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