Archives of oral biology最新文献

{"title":"Masticatory function and three-dimensional facial morphology of soft tissues: One year after orthognathic surgery","authors":"Joana Carolina Martins Simões ,&nbsp;Denny Marcos Garcia ,&nbsp;Francisco Veríssimo De Mello-Filho ,&nbsp;Claudia Maria De Felício ,&nbsp;Luciana Vitaliano Voi Trawitzki","doi":"10.1016/j.archoralbio.2024.106103","DOIUrl":"10.1016/j.archoralbio.2024.106103","url":null,"abstract":"<div><h3>Objective</h3><div>Dentofacial deformities (DFD) require orthodontic treatment, orthognathic surgery, and speech therapy for aesthetic and functional problems. This longitudinal study analyzed changes in masticatory function and three-dimensional (3D) facial soft tissue in patients with Class II and Class III DFD after orthognathic surgery. In addition, the study investigated the relationship between facial measurements, maximum bite force (MBF), and orofacial myofunctional status (OMS).</div></div><div><h3>Design</h3><div>The sample consisted of 46 participants, including 10 patients with Class II DFD, and 11 patients with Class III DFD. These groups were assessed before (T0) and 6 months (T1) after surgery. Twelve patients who completed the treatment protocol were evaluated 12 months post-surgery (T2). The patient groups were compared with each other and with a control group (CG) of 25 healthy subjects. The participants underwent MBF and clinical evaluation of OMS, including masticatory behavior, using a validated protocol. The 3D facial soft tissue was assessed using laser scanning.</div></div><div><h3>Results</h3><div>Compared to the CG, DFD patients showed reduced MBF, masticatory behavior, and OMS scores at T0. At T2, there was an improvement in MBF, masticatory, and OMS scores, but differences were observed compared to the CG. Changes in facial soft tissue followed the underlying hard tissue movement resulting from surgery, and reduction of vertical measurements contributed to improvements in masticatory function.</div></div><div><h3>Conclusion</h3><div>These findings indicated that combined surgery, orthodontic treatment, and speech therapy can lead to a significant improvement in masticatory function and facial soft tissue in DFD patients, although not completely 12 months post-surgery.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106103"},"PeriodicalIF":2.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intriguing astrocyte responses in CA1 to reduced and rehabilitated masticatory function: Dorsal and ventral distinct perspectives in adult mice","authors":"Micaele Maria Lopes Castro ,&nbsp;Fabio Leite do Amaral Junior ,&nbsp;Fabíola de Carvalho Chaves de Siqueira Mendes ,&nbsp;Daniel Clive Anthony ,&nbsp;Dora Maria Tuna de Oliveira Brites ,&nbsp;Cristovam Wanderley Picanço Diniz ,&nbsp;Marcia Consentino Kronka Sosthenes","doi":"10.1016/j.archoralbio.2024.106097","DOIUrl":"10.1016/j.archoralbio.2024.106097","url":null,"abstract":"<div><h3>Objective</h3><div>We sought to investigate the plasticity of diet-induced changes in astrocyte morphology of <em>stratum lacunosum-moleculare (SLM)</em> in CA1.</div></div><div><h3>Design</h3><div>Three diet regimes were adopted in 15 mice, from the 21st postnatal day to 6 months. The first diet regimen was pellet feed, called Hard Diet (HD). The second, with reduced masticatory, received a pellet-diet followed by a powdered-diet, and it was identified as Hard Diet/Soft Diet (HD/SD). Finally, the group with rehabilitated masticatory was named Hard Diet/Soft Diet/Hard Diet (HD/SD/HD). In the end, euthanasia and brain histological processing were performed, in which astrocytic immunoreactivity to glial-fibrillary-acidic-protein (GFAP) was tested. In reconstructed astrocytes, morphometric analysis was performed.</div></div><div><h3>Results</h3><div>Astrocyte morphometric revealed that changes in masticatory regimens impact astrocyte morphology. In the dorsal CA1, switching from a hard diet to a soft diet led to reductions in most variables, whereas in the ventral, fewer variables were affected, highlighting regional differences in astrocyte responses. Cluster analysis further showed that diet-induced changes in astrocyte morphology were reversible in the dorsal region, but not in the ventral region, indicating a persistent impact on astrocyte diversity and complexity in the ventral even after rehabilitation. Correlation tests between astrocyte morphology and behavioral performance demonstrated disrupted relationships under masticatory stress, with effects persisting after rehabilitation.</div></div><div><h3>Conclusion</h3><div>Changes in the diet result in significant alterations in astrocyte morphology, suggesting a direct link between dietary modulation and cellular structure. Morphometric analyses revealed distinct alterations in astrocyte morphology in response to changes in the masticatory regimen, with both dorsal/ventral regions displaying notable changes. Moreover, the regional differential effects on astrocytes underscore the complexity of mastication on neuroplasticity and cognitive function.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106097"},"PeriodicalIF":2.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Familial hypodontia in bronze age Northwest China (1046–771BC)","authors":"Yaohan Wu ,&nbsp;Qiang Ma ,&nbsp;Baiwei Han ,&nbsp;Yuanyuan Shen ,&nbsp;Shaoqing Wen","doi":"10.1016/j.archoralbio.2024.106104","DOIUrl":"10.1016/j.archoralbio.2024.106104","url":null,"abstract":"<div><h3>Objective</h3><div>This research aimed to report hypodontia cases in a Middle Bronze Age high-tier cemetery in China and test the possible hereditary behind hypodontia by performing kinship tests on those individuals.</div></div><div><h3>Design</h3><div>In this study, dental anomalies were observed on a human skeletal sample (n = 45) uncovered from Yaoheyuan, China. Ancient DNA analysis was subsequently employed on a subsample of the Yaoheyuan individuals (n = 15), including individuals observed hypodontia and individuals randomly sampled from the cemetery for preliminary investigation on the cemetery demography. Kinship estimation tests (READ, TKGWV2, KIN, and F3 test) were subsequently employed.</div></div><div><h3>Results</h3><div>The Yaoheyuan elite population had a prevalence (n = 7, 15 %) of tooth agenesis in either the maxilla or mandible, with one to two teeth missing. All missing teeth were incisors, except for one individual missing maxillary second molar. Preliminary ancient DNA results indicate that several kinship groups existed among interred individuals, including those with hypodontia, indicating the hereditary origin of these cases.</div></div><div><h3>Conclusions</h3><div>The prevalence of hypodontia observed on site is high compared to that in both modern East Asian populations and archaeological samples in the Chinese population. The preliminary kinship analysis suggests a case of familial hypodontia. Ancient DNA analysis should be thoroughly conducted in future studies to understand the genetic markers contributing to those hypodontia cases among the Yaoheyuan individuals.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106104"},"PeriodicalIF":2.2,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142395872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic insights into idiopathic xerostomia: The central role of caffeine metabolism in salivary biochemistry","authors":"Feifei Hou ,&nbsp;Yuan Cui ,&nbsp;Lu Ye ,&nbsp;Fangman Chen ,&nbsp;Chuanji Wu ,&nbsp;Yang Meng ,&nbsp;Peiyang Yuan ,&nbsp;Xuemei Qiu ,&nbsp;Xiaodong Feng ,&nbsp;Lu Jiang","doi":"10.1016/j.archoralbio.2024.106102","DOIUrl":"10.1016/j.archoralbio.2024.106102","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to delineate the salivary metabolomic profile of patients with idiopathic xerostomia using untargeted metabolomics techniques, with the goal of addressing the lack of clear diagnostic markers and providing insights into the pathophysiological mechanisms of the condition.</div></div><div><h3>Design</h3><div>In this observational, cross-sectional study, saliva samples from 33 patients with idiopathic xerostomia and 34 healthy controls were analyzed using Ultra Performance Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry (UPLC-QTOF MS). Metabolomic profiling was complemented by multivariate statistical analysis to differentiate between affected individuals and controls.</div></div><div><h3>Results</h3><div>Metabolomic analysis delineated a pronounced differentiation between patients with idiopathic xerostomia and healthy controls. A total of 195 metabolites displayed significant differential expression, each with a variable importance in projection (VIP) greater than 1 and a <em>P</em>-value less than 0.05. Pathway enrichment analysis, according to the Kyoto Encyclopedia of Genes and Genomes (KEGG), identified 22 metabolites that participated in 18 distinct metabolic pathways. Among these, the caffeine metabolism pathway, characterized by notable alterations in impact values (VIP, <em>P</em>-value, Log2-fold change, Rich factor), emerged as the most significantly disrupted, underscoring its potential role in the pathophysiology of idiopathic xerostomia (<em>P</em> = 0.0000395).</div></div><div><h3>Conclusions</h3><div>The salivary metabolomic profiling revealed distinct alterations in idiopathic xerostomia, with a significant reduction in caffeine metabolism pathways, underscoring potential neuropathic involvement. This study advances our understanding of the metabolic alterations in xerostomia, suggesting that salivary metabolomics may offer viable biomarkers for diagnosing and understanding the etiology of idiopathic xerostomia. Future research should focus on therapeutic targeting of these metabolic disturbances and evaluating their reversibility with treatment.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106102"},"PeriodicalIF":2.2,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of preservatives on the activities of salivary enzymes","authors":"Moon-Jong Kim ,&nbsp;Ji-Youn Chang ,&nbsp;Yoon-Young Kim ,&nbsp;Jae Wook Lee ,&nbsp;Hong-Seop Kho","doi":"10.1016/j.archoralbio.2024.106098","DOIUrl":"10.1016/j.archoralbio.2024.106098","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the effects of common preservatives used in oral health care products on the enzymatic activities of lysozyme, peroxidase, and α-amylase in-solution and on-hydroxyapatite surface phases.</div></div><div><h3>Design</h3><div>The preservatives used in this study were sodium benzoate, methylparaben, propylparaben, and benzalkonium chloride. Hen egg-white lysozyme, bovine lactoperoxidase, and α-amylase from <em>Bacillus</em> sp. served as sources of purified enzymes. Human unstimulated whole saliva was used as a source of salivary enzymes. Hydroxyapatite beads were used as the surface phase. The preservatives were incubated with purified enzymes or saliva samples in-solution or on-hydroxyapatite surface phases, respectively. Enzymatic activities of lysozyme, peroxidase, and α-amylase were measured by hydrolysis of fluorescein-labelled <em>Micrococcus lysodeikticus</em>, oxidation of fluorogenic 2′,7′-dichlorofluorescin, and hydrolysis of fluorogenic starch, respectively.</div></div><div><h3>Results</h3><div>The effects of the preservatives on the enzymatic activities of lysozyme and peroxidase were more distinct in the saliva samples than purified substances, and in the in-solution phase than on-hydroxyapatite surface phase, and the opposite was true for α-amylase. The most significant result was apparent decrease in peroxidase activities caused by the parabens in the in-solution phase (<em>P</em>&lt;0.05). Sodium benzoate and parabens inhibited lysozyme activity in the in-solution phase, but differently for the purified and salivary lysozymes. Parabens and benzalkonium chloride inhibited the enzymatic activity of α-amylase from <em>Bacillus</em> sp., not saliva samples, only on-hydroxyapatite surface (<em>P</em>&lt;0.05).</div></div><div><h3>Conclusions</h3><div>Each preservative affected the enzymatic activities of lysozyme, peroxidase, and α-amylase differently. Based on the effects on salivary enzymes, sodium benzoate or benzalkonium chloride was recommended as preservatives rather than parabens.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106098"},"PeriodicalIF":2.2,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142376432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of temporomandibular disorder on food preference behavior in adult male rats","authors":"Saeid Ghorbanizadeh ,&nbsp;Mehdi Abbasnejad ,&nbsp;Mahnaz Zamyad ,&nbsp;Saeed Esmaeili-Mahani ,&nbsp;Reza Kheirandish ,&nbsp;Mojdeh Mansoori","doi":"10.1016/j.archoralbio.2024.106100","DOIUrl":"10.1016/j.archoralbio.2024.106100","url":null,"abstract":"<div><h3>Objective</h3><div>Temporomandibular disorders (TMD) are a group of conditions affecting the jaw, surrounding muscles and associated structures. Researches indicate that TMD has implications for changes in nutritional behavior. This study investigates the impact of TMD on the food preference behavior of rats, examining various food models with differing caloric content, taste, and texture.</div></div><div><h3>Design</h3><div>Forty-two male Wistar rats (200–250 g) were divided into six groups, comprising three control and three pain or TMD groups. Induction of TMD by injection of complete Freund adjuvant (CFA) into the left temporomandibular joint (TMJ) of rat serves as a model for studying TMD. Twenty-four hours post-TMD induction, the preference of animals to foods with differences in calories, taste and texture was evaluated with the help of an automatic preference measuring device.</div></div><div><h3>Results</h3><div>In terms of caloric preferences, the pain group exhibited a distinct shift towards high-calorie food. Taste preferences were marked by an increased preference for sweet food in the pain group. Texture preferences were altered, with the pain group displaying a preference for low-texture food.</div></div><div><h3>Conclusion</h3><div>TMD in rats leads to increased preferences for high-calorie and sweet foods, as well as altered preferences for food textures. These findings highlight the influence of TMJ pain on food preference behavior in rats.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106100"},"PeriodicalIF":2.2,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of chronic unpredictable mild stress-induced depression on bitter taste receptor expression in mice","authors":"Zhaoxiang Wang ,&nbsp;Min Zhang ,&nbsp;Meng Huang ,&nbsp;Luyue Zhang ,&nbsp;Gencheng Han ,&nbsp;Ge Li ,&nbsp;Junkai Cao","doi":"10.1016/j.archoralbio.2024.106099","DOIUrl":"10.1016/j.archoralbio.2024.106099","url":null,"abstract":"<div><h3>Objective</h3><div>With the rapid increase in the pace of life, people are facing increasing pressures of all kinds, and depression has gradually become a serious psychological disorder in human society, strongly affecting normal social and physiological activities. Depression can disrupt an individual's taste perception and potentially result in taste disorders by affecting and altering taste receptors. This disruption can consequently impact their food preferences and overall eating experiences.</div></div><div><h3>Design</h3><div>In this study, we used the chronic unpredictable mild stress (CUMS) method to establish a depression model in male C57BL/6 J mice and explored the changes in taste receptor expression in the lingual circumvallate papillae (CP) to elucidate the effects of depression on taste. After 6 weeks of CUMS, behavioral performance evaluations, such as forced swim, open field, and elevated plus maze tests, were conducted in depression model mice. A further two-bottle choice test was subsequently performed to determine the effect of depression on bitter taste, and the expression of bitter taste receptors in the lingual CP was detected via immunofluorescence staining.</div></div><div><h3>Results</h3><div>In this study, we found for the first time that mice with CUMS-induced depression had decreased bitter taste sensitivity through a two-bottle choice test and demonstrated that the expression of T2r5, a receptor related to bitter taste perception, and the expression of secondary taste signaling proteins in the lingual CP were significantly decreased in mice exposed to CUMS, as determined via qRT<img>PCR and immunofluorescence staining.</div></div><div><h3>Conclusions</h3><div>Our study highlights how CUMS influences the perception of bitterness in the peripheral taste system, potentially elucidating stress-induced changes in eating habits.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106099"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An intronic variant in LAMB3 contributes to junctional epidermolysis bullosa and enamel hypoplasia via translational attenuation","authors":"Yi Yang ,&nbsp;Yao Wang ,&nbsp;Man Qin ,&nbsp;Yuming Zhao ,&nbsp;Cristina Has ,&nbsp;Xin Wang","doi":"10.1016/j.archoralbio.2024.106101","DOIUrl":"10.1016/j.archoralbio.2024.106101","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate the genetic etiology of a family affected by junctional epidermolysis bullosa (JEB) and generalized enamel hypoplasia, and to explore how an intronic variant influenced the 5’ untranslated region (5’UTR), thereby affecting LAMB3 expression and contributing to the pathogenesis of the disease.</div></div><div><h3>Design</h3><div>Whole-exome and whole-genome sequencing were used to screen for genetic defects in the patient. Mutational consequences were characterized through luciferase assays, splice assay, in silico analyses, and verification using the patient's gingival sample.</div></div><div><h3>Results</h3><div>A nonsense variant (c.2983 C&gt;T; p.Gln995*) and an intronic variant (c.–38+2 T&gt;C) of <em>LAMB3</em> were identified. <em>In vitro</em> assays demonstrated that the intronic variant activated a cryptic splice site, resulting in a 120 bp intronic inclusion. This splicing alteration significantly reduced the translation efficiency of the downstream coding sequence, while overall mRNA expression remained unaffected. Bioinformatic analysis unveiled the creation of three upstream AUG codons, leading to the presence of two upstream open reading frames (uORFs) and one overlapping ORF. The longer uORF's AUG exhibited a moderate Kozak strength similar to that of the main ORF's AUG. Structural analysis of the mutant 5’UTR sequence revealed a more complex secondary structure, characterized by a large branch loop and a stem-loop preceding the coding sequence's start codon.</div></div><div><h3>Conclusion</h3><div>This study suggests that variants affecting the 5’UTR may contribute to the genetic etiology of JEB. These findings could help enhance the diagnostic accuracy and efficiency in JEB patients.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106101"},"PeriodicalIF":2.2,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roles of the histone methyltransferase SET domain bifurcated 1 in epithelial cells during tooth development","authors":"Yuri Takagiwa,&nbsp;Norihisa Higashihori,&nbsp;Sakurako Kano,&nbsp;Keiji Moriyama","doi":"10.1016/j.archoralbio.2024.106094","DOIUrl":"10.1016/j.archoralbio.2024.106094","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to reveal the effects of SET domain bifurcated 1 (SETDB1) on epithelial cells during tooth development.</div></div><div><h3>Design</h3><div>We generated conditional knockout mice (<em>Keratin 14-Cre;Setdb1</em>^<em>f/f</em> mice), in which <em>Setdb1</em> was deleted only in epithelial cells. At embryonic day 14.5 (E14.5), immunofluorescence staining was performed to confirm the absence of SETDB1 within the epithelium of tooth embryos from <em>Keratin 14-Cre;Setdb1</em>^<em>f/f</em> mice. Mouse embryos were harvested after reaching embryonic day 13.5 (E13.5), and sections were prepared for histological analysis. To observe tooth morphology in detail, electron microscopy and micro-CT analysis were performed at postnatal months 1 (P1M) and 6 (P6M). Tooth embryos were harvested from postnatal day 7 (P7) mice, and the epithelial components of the tooth embryos were isolated and examined using quantitative RT-PCR for the expression of genes involved in tooth development.</div></div><div><h3>Results</h3><div><em>Keratin 14-Cre;Setdb1</em>^<em>f/f</em> mice exhibited enamel hypoplasia, brittle and fragile dentition, and significant abrasion. Coronal sections displayed abnormal ameloblast development, including immature polarization, and a thin enamel layer that detached from the dentinoenamel junction at P7. Electron microscopic analysis revealed characteristic findings such as an uneven surface and the absence of an enamel prism. The expression of <em>Msx2</em>, <em>Amelogenin (Amelx), Ameloblastin (Ambn), and Enamelin (Enam)</em> was significantly downregulated in the epithelial components of tooth germs in <em>Keratin 14-Cre;Setdb1</em>^<em>f/f</em> mice.</div></div><div><h3>Conclusions</h3><div>These results indicate that SETDB1 in epithelial cells is important for tooth development and clarify the relationship between the epigenetic regulation of SETDB1 and amelogenesis imperfecta for the first time.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106094"},"PeriodicalIF":2.2,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of regenerative responses by retinoic and ascorbic acids in human apical papilla cells","authors":"Lídia de Oliveira Fernandes ,&nbsp;Igor Paulino Mendes Soares ,&nbsp;Rafael Antonio de Oliveira Ribeiro ,&nbsp;Victória Peruchi ,&nbsp;Maria Luiza Barucci Araujo Pires ,&nbsp;Caroline Anselmi ,&nbsp;Maria Luisa Leite ,&nbsp;Carlos Alberto de Souza Costa ,&nbsp;Josimeri Hebling","doi":"10.1016/j.archoralbio.2024.106095","DOIUrl":"10.1016/j.archoralbio.2024.106095","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the bioactive effects of retinoic acid and ascorbic acid on hSCAPs in vitro.</div></div><div><h3>Design</h3><div>Cells were obtained from human third molars (n=4) and characterized for mesenchymal stem cell markers by flow cytometry. The experimental groups: control (α-MEM); vehicle control group (α-MEM + 0.17 % DMSO); retinoic acid 0.1, 1, and 10 µM; and ascorbic acid 3, 30, and 300 µM (n=8) were tested for cell viability (alamarBlue; 1, 3, and 7 days), total collagen synthesis (Sirius Red; 1 and 7 days), mineralized matrix formation (Alizarin red; 14 days), and the regulation of gene expression related to mineralization (<em>ALPL</em> and <em>DSPP</em>), cell migration (<em>ITGAV</em> and <em>CXCL12</em>) angiogenesis (<em>VEGFA)</em> and collagen synthesis (<em>COL1A1</em> and <em>COL3A1;</em> RT-qPCR) on 1 and 7 days. <em>ACTB</em> and <em>GAPDH</em> were used as reference genes. Data were analyzed by ANOVA and complementary tests at a 5 % significance level.</div></div><div><h3>Results</h3><div>Ascorbic acid 300 µM increased viability, and retinoic acid reduced it dose-dependently. Retinoic acid 0.1 µM and ascorbic acid 30 and 300 µM increased mineralized matrix formation and total collagen synthesis, and retinoic acid 10 µM decreased. On day 1, 0.1 µM retinoic acid upregulated the gene expression of <em>COL1A1</em>, <em>COL3A1</em>, <em>VEGFA</em>, <em>CXCL12</em>, <em>ALPL</em>, <em>DSPP</em> e <em>ITGAV</em>, and 300 µM ascorbic acid upregulated <em>COL1A1</em>, <em>COL3A1</em> and <em>DSPP</em>. However, on day 7, retinoic acid downregulated <em>ALPL</em>, <em>COL3A1</em>, <em>CXCL12</em>, and VEGFA and downregulated <em>ITGAV</em> and <em>VEGFA</em>.</div></div><div><h3>Conclusion</h3><div>Retinoic acid 0.1 µM and ascorbic acid 300 µM biostimulated hSCAPs to differentiate into pro-regenerative phenotypes with potential application for REPs.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106095"},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142367842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}