Archives of oral biology最新文献

{"title":"Necroptosis and its role in the pathogenesis and treatment of temporomandibular joint osteoarthritis: A narrative review","authors":"Miaomiao Zhao ,&nbsp;Luyao Song ,&nbsp;Zhichao Liu,&nbsp;Yuting Li,&nbsp;Yingnan Wang","doi":"10.1016/j.archoralbio.2025.106300","DOIUrl":"10.1016/j.archoralbio.2025.106300","url":null,"abstract":"<div><h3>Objective</h3><div>This review aimed to explore the role of necroptosis in the pathogenesis and treatment of temporomandibular joint osteoarthritisjoints. We sought to elucidate the mechanisms by which necroptosis contributes to arthritis development and identify potential therapeutic targets within the necroptosis pathway.</div></div><div><h3>Design</h3><div>An electronic literature search was conducted in PubMed, Scopus, EBSCO, ProQuest, ScienceDirect, and Springer for studies on necroptosis, arthritis, and temporomandibular joint osteoarthritis published between 2004 and 2024.</div></div><div><h3>Results</h3><div>Necroptosis, a programmed cell death pathway characterized by the activation of receptor-interacting protein kinase 1/3, leads to cell membrane rupture and the release of damage-associated molecular patterns, which are instrumental in promoting inflammation. This review highlights the involvement of necroptosis in the progression of multiple types of arthritis, especially temporomandibular joint osteoarthritis. These findings highlight the potential of necroptosis inhibitors as therapeutic agents, with several small-molecule inhibitors demonstrating efficacy in preclinical models of arthritis.</div></div><div><h3>Conclusions</h3><div>Necroptosis is a significant factor in the pathogenesis of arthritis, particularly temporomandibular joint osteoarthritis, and represents a promising therapeutic target. Targeting the necroptosis pathway may offer a novel approach for managing joint damage and osteochondral inflammation. Further research is necessary to fully understand the mechanisms of necroptosis in temporomandibular joint osteoarthritis and to develop targeted therapies for clinical application.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106300"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of optimised sample preparation method on DNA recovery from subgingival plaque in a population with periodontitis","authors":"Pasquale Santamaria,&nbsp;Mandeep Ghuman,&nbsp;Luigi Nibali","doi":"10.1016/j.archoralbio.2025.106299","DOIUrl":"10.1016/j.archoralbio.2025.106299","url":null,"abstract":"<div><h3>Objective(s)</h3><div>This study aimed to evaluate whether an alternate sample preparation method could enhance DNA yield from subgingival plaque samples collected from patients with untreated periodontitis.</div></div><div><h3>Design</h3><div>Fifty-six consecutive participants with untreated periodontitis each provided two subgingival plaque samples from periodontal sites with probing pocket depth (PPD) &gt; 5 mm. In the alternate method (AM), 1.4 mm ceramic beads were added to the sample prior to supernatant removal to protect the microbial pellet, after which samples were processed using a standardized extraction protocol and subjected to 16S rRNA gene sequencing (V3–V4 region).</div></div><div><h3>Results</h3><div>A total of 112 samples were analysed. DNA concentration (ng/µl) was significantly higher in the AM group (23.82 ± 23.31) compared to the standard method (SM) group (13.6 ± 17.07; p &lt; 0.001). The DNA input in 2.5 µl was also significantly greater in the AM group (1156 ± 1139 ng vs. 277.96 ± 273.12 ng; p &lt; 0.001). Shannon diversity was significantly higher in AM than SM (p = 6e–04). No significant differences in DNA yield or microbial diversity were observed between the two periodontal sites within each group.</div></div><div><h3>Conclusion</h3><div>The alternate sample preparation method improved DNA concentration compared to standard sample preparation method of sub-gingival plaque analysis collected from patients with untreated periodontitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106299"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondroprotective role of histone deacetylase 4 in early-stage of temporomandibular joint osteoarthritis in male rats","authors":"Qi Ning ,&nbsp;Weihua Han ,&nbsp;Juanhong Meng ,&nbsp;Yehua Gan","doi":"10.1016/j.archoralbio.2025.106301","DOIUrl":"10.1016/j.archoralbio.2025.106301","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to investigate the expression and role of histone deacetylase 4 (<em>Hdac4</em>) in temporomandibular joint osteoarthritis in rats.</div></div><div><h3>Design</h3><div>A model of temporomandibular joint (TMJ) osteoarthritis was established by partial discectomy in male rats. In the early stage of TMJ osteoarthritis, small interfering-HDAC4 (si-HDAC4) with adeno-associated virus was injected into the rat articular cavity to knockdown the expression of <em>Hdac4</em> in TMJ. And an adenoviral vector was used to transfer <em>Hdac4</em> into the rat articular cavity to increase the level of <em>Hdac4</em> in TMJ. Subsequently, the pathological changes and stages of condylar cartilage were examined histopathologically at different time points after injection respectively, and the microstructure of TMJ subchondral bone was evaluated using micro computed tomography.</div></div><div><h3>Results</h3><div><em>Hdac4</em> expression in condylar cartilage was upregulated in the early-stage of TMJ osteoarthritis, but decreased in the mid to late-stage. Knockdown of <em>Hdac4</em> expression in the early-stage of TMJ osteoarthritis accelerated the destruction of condylar cartilage in vivo, while overexpression of <em>Hdac4</em> reduced the destruction of cartilage in the late-stage. Meanwhile, on micro computed tomography, the microstructure of subchondral bone in TMJ showed that overexpression of <em>Hdac4</em> led to a decrease in incidence of bone sclerosis.</div></div><div><h3>Conclusions</h3><div>Our results suggested that <em>Hdac4</em> has a certain chondroprotective effect in the development of TMJ osteoarthritis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106301"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PCSK9 is a passenger gene in head and neck cancer with minimal pathological influence","authors":"Hyun-Ji Kim ,&nbsp;Dong-Guk Park ,&nbsp;Su-Jung Choi ,&nbsp;Sung-Dae Cho","doi":"10.1016/j.archoralbio.2025.106302","DOIUrl":"10.1016/j.archoralbio.2025.106302","url":null,"abstract":"<div><h3>Objectives</h3><div>In this study, we aimed to explore the pathological effects of PCSK9 in head and neck cancer (HNC) by ablating its expression using the CRISPR-Cas9 knockout system.</div></div><div><h3>Design</h3><div>To investigate the clinical importance of PCSK9 in HNC, <em>in silico</em> analysis was performed using datasets from the GEO database and the UALCAN database. To evaluate the role of PCSK9 in HNC pathogenesis, both CRISPR-Cas9 knockout system and pharmacological inhibition were employed to ablate PCSK9 expression in HNC cell lines. The impact of PCSK9 on cellular growth and proliferation was assessed using Cell Counting Kit-8, soft agar, and clonogenic assays in a 2D culture model, as well as a hanging drop spheroid formation assay with live/dead staining in a 3D culture model. The involvement of PCSK9 in apoptosis induction was evaluated by detecting c-PARP expression through Western blotting, measuring the sub-G1 population via cell cycle assay, and verifying the Annexin V-positive population. Finally, changes in metastatic profiles associated with fluctuations in PCSK9 expression were examined using wound healing and transwell migration/invasion assays.</div></div><div><h3>Results</h3><div>In <em>in silico</em> analysis results, PCSK9 appeared to be related to the progression of HNC. However, experimental results demonstrated that PCSK9 plays a minimal role in cancer cell proliferation, anchorage-independent growth, colony formation capacity, in 2D cultures, as well as spheroidal growth in 3D cultures, and apoptosis induction. Furthermore, PCSK9 marginally influenced wound closure, metastatic potential, and invasive ability.</div></div><div><h3>Conclusion</h3><div>Collectively, these data suggest that PCSK9 serves a neutral role in HNC, functioning as a passenger gene.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106302"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abscisic acid ameliorates cognitive dysfunctions, but not facial allodynia, in a male rat nitroglycerin migraine model","authors":"Alireza Ahmadi ,&nbsp;Monavareh Soti ,&nbsp;Mohammad Reza Zarei ,&nbsp;Kristi Anne Kohlmeier ,&nbsp;Mohammad Shabani","doi":"10.1016/j.archoralbio.2025.106280","DOIUrl":"10.1016/j.archoralbio.2025.106280","url":null,"abstract":"<div><h3>Objective</h3><div>Abscisic acid (ABA), found in fruits and vegetables and produced in the human body, has anti-inflammatory, anti-oxidative, and pro-cognitive effects. The impact of ABA on trigeminal nerve hyperalgesia and cognitive behaviors in a rat migraine model was evaluated.</div></div><div><h3>Design</h3><div>Male rats (n = 8) were randomly distributed into 5 groups (Control, Sham, Abscisic acid (ABA), Nitroglycerin (NTG), and Nitroglycerin + Abscisic acid (NTG+ABA). Migraine was induced through 4 injections of NTG (5 mg/kg intraperitoneal (i.p.)). ABA (10 <em>µ</em>g/rat intracerebroventricular (i.c.v)) was administered 4 times with an interval of 24 hours. To evaluate trigeminal nerve hyperalgesia, the von Frey, acetone, and air puff tests were used. To evaluate locomotor and cognitive function of the animals, the open field, elevated plus maze (EPM), tail suspension (TST), Morris water maze (MWM), and passive avoidance (PA) tests were administered. Total antioxidant capacity levels (n = 5; TAC) were also monitored.</div></div><div><h3>Results</h3><div>Significant differences were observed between the surgically treated groups and the control group, but no differences in pain indexes were found in migraine animals when ABA was present. ABA-treated animals among the migraine groups showed reductions in anxiety-like and depression-like behaviors. Additionally, in the MWM test, the ABA animals exhibited improved spatial learning, while their memory did not show improvement. Avoidance learning and memory were not affected when evaluated using the PA. Increases in TAC were seen in ABA-treated animals in both serum and the spinal trigeminal nucleus.</div></div><div><h3>Conclusion</h3><div>Taken together, ABA reduces NTG-induced cognitive impairments, and antioxidant activity could play a role in the underlying ABA-mediated mechanism.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106280"},"PeriodicalIF":2.2,"publicationDate":"2025-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anaphase promoting complex subunit 10 is a potential diagnostic and prognostic biomarker in oral squamous cell carcinoma","authors":"Lu Jia ,&nbsp;Haoyong Ning ,&nbsp;Ying Liu ,&nbsp;Pan Yang ,&nbsp;Xiaowei Cheng ,&nbsp;Bin Wang","doi":"10.1016/j.archoralbio.2025.106296","DOIUrl":"10.1016/j.archoralbio.2025.106296","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the role of anaphase promoting complex subunit 10 (ANAPC10) in both the diagnosis and prognosis of oral squamous cell carcinoma (OSCC).</div></div><div><h3>Design</h3><div><em>ANAPC10</em> expressions in OSCC tissues and adjacent normal tissues were analysed using TCGA and GEO databases. Its clinical prognostic significance was evaluated using the GEPIA tool. Signalling pathways associated with <em>ANAPC10</em> were identified through GO, KEGG, and GSEA. Promoter methylation levels of <em>ANAPC10</em> were assessed using the UALCAN tool. The correlation between <em>ANAPC10</em> expression and tumour-infiltrating immune cells was analysed using the TIMER database. <em>ANAPC10</em>’s role in OSCC cells was validated via CCK-8 assays, wound healing assays, cell migration assays, apoptosis assays, and cell cycle analysis.</div></div><div><h3>Results</h3><div><em>ANAPC10</em> expression was significantly elevated in OSCC tissues. Increased <em>ANAPC10</em> expression was associated with advanced T stages, pathological stages, histologic grades, and poorer therapeutic outcomes. Notably, high <em>ANAPC10</em> expression was strongly correlated with reduced overall survival, disease-specific survival, and progression-free interval in OSCC patients. Functional enrichment analyses revealed that <em>ANAPC10</em> is involved in RNA splicing, immune regulation, and cell cycle progression. Experimental validation further demonstrated that <em>ANAPC10</em> levels are influenced by promoter methylation status, and ANAPC10 regulates oral cancer cell proliferation, migration, apoptosis, and cell cycle progression.</div></div><div><h3>Conclusion</h3><div>ANAPC10 is a critical gene in OSCC prognosis, with roles in cell cycle regulation and RNA splicing. It may serve as a diagnostic biomarker and therapeutic target.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106296"},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144106625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zinc-alpha-2-glycoprotein overexpression and maintaining anti-apoptotic function in oral squamous cell carcinoma","authors":"Jureeporn Chuerduangphui ,&nbsp;Tipaya Ekalaksananan ,&nbsp;Chukkris Heawchaiyaphum ,&nbsp;Patravoot Vatanasapt ,&nbsp;Watchareporn Teeramatwanich ,&nbsp;Pensiri Phusingha ,&nbsp;Chamsai Pientong","doi":"10.1016/j.archoralbio.2025.106298","DOIUrl":"10.1016/j.archoralbio.2025.106298","url":null,"abstract":"<div><h3>Objective</h3><div>Overexpression of zinc-alpha-2-glycoprotein (ZAG) can be induced by various factors and has potential to be a biomarker in certain malignancies. However, in oral squamous cell carcinoma (OSCC), the risks and effects associated with ZAG overexpression are still poorly known. Here, we investigated the effect of HPV16 oncogenes and arecoline on the expression levels of ZAG and the possible effects of ZAG in OSCC cell lines.</div></div><div><h3>Design</h3><div>The level of ZAG expression was determined in protein extracted from exfoliated buccal cells from cancer-free control individuals and oral lesion cells from OSCC. Oral cell lines expressing HPV16E6/E7, and treated with arecoline were prepared to investigate ZAG expression. The effects of ZAG on cell biological activity and its targeting of UCP1 were determined in ZAG-overexpressing and ZAG-knockdown cells. <em>Results:</em> The expression of ZAG protein was significantly increased in oral lesion cells from OSCC relative to controls. Notably, the expression level of ZAG in OSCC positive for HPV, betel-quid chewing, and combination of both factors, was slightly higher than in cancer-free controls. ZAG expression was upregulated in oral cells treated with HPV16 oncoproteins E6 and/or E7, and treatment with arecoline (25 μg/ml). Interestingly, ZAG overexpression significantly increased <em>UCP1</em> and decreased apoptosis, whereas decreased <em>UCP1</em> and increased apoptosis were found in ZAG-knockdown cells. The mRNA expression levels of <em>TP53</em>, <em>STAT3</em>, <em>BCL2</em>, and <em>NFKB1</em> corresponded to observed anti-apoptosis function.</div></div><div><h3>Conclusions</h3><div>HPV oncoproteins and high doses of arecoline are risk factors for an overexpressed ZAG protein that has an anti-apoptotic function in OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106298"},"PeriodicalIF":2.2,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branched-chain amino acids promote gelatinase secretion from human periodontal ligament stem cells through nuclear factor kappa-B signaling","authors":"Xinjie Ning ,&nbsp;Huiling Zheng ,&nbsp;Ying Tu ,&nbsp;Qiang Guo ,&nbsp;Biao Ren ,&nbsp;Leng Wu ,&nbsp;Jing Xie ,&nbsp;Chengcheng Liu","doi":"10.1016/j.archoralbio.2025.106297","DOIUrl":"10.1016/j.archoralbio.2025.106297","url":null,"abstract":"<div><h3>Objective</h3><div>To explore the effects of branched-chain amino acids (BCAAs) on periodontal tissues and regulation of gelatinase secretion by human periodontal ligament stem cells (hPDLSCs).</div></div><div><h3>Design</h3><div>The salivary BCAA levels (leucine, isoleucine, and valine) in the clinical participants were measured using mass spectrometry. A local injection model in the periodontium of Sprague Dawley rats was established to investigate the periodontal destruction induced by BCAAs. A BCAA-treatment model of hPDLSCs was established to detect the expression and activity of gelatinase and further explore the potential mechanism by which BCAAs enhance gelatinase secretion.</div></div><div><h3>Results</h3><div>Compared to the healthy controls, the salivary levels of leucine (<em>p</em> = 0.0190), isoleucine (<em>p</em> = 0.0351), and valine (<em>p</em> = 0.0072) were significantly elevated in individuals with periodontitis. <em>In vivo</em> experiments revealed that BCAAs aggravated periodontal extracellular matrix degradation and alveolar bone resorption in rats. Three-dimensional reconstruction of the rat maxilla demonstrated an increase in the distance from the cementoenamel junction to the alveolar bone crest (<em>p</em> &lt; 0.0001), and a decrease in the bone volume fraction (<em>p</em> &lt; 0.0001). <em>In vitro</em> experiments demonstrated that BCAAs activate the phosphorylation of nuclear factor kappa-B (NF-κB) signaling pathway in the hPDLSCs and consequently induce the secretion of gelatinases. The absence of any of the components in the BCAAs attenuated this effect.</div></div><div><h3>Conclusion</h3><div>BCAAs increase gelatinase secretion through the NF-κB (p-p65) signaling pathway, consequently exacerbating periodontal tissue destruction. This provides a novel insight on the role of BCAAs in the host immune-inflammatory response and increases our understanding of the possible involvement of BCAAs in the periodontitis development.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106297"},"PeriodicalIF":2.2,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143947648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Porphyromonas gingivalis lipopolysaccharide on the behavior of human dental pulp stem cells in vitro and their inflammation-related transcriptomics profile","authors":"Fei He ,&nbsp;Jingya Zhu ,&nbsp;Xiangni Zeng ,&nbsp;Li Jiang ,&nbsp;Lixia Zhang","doi":"10.1016/j.archoralbio.2025.106295","DOIUrl":"10.1016/j.archoralbio.2025.106295","url":null,"abstract":"<div><h3>Objective</h3><div>The inflammatory microenvironment in pulpitis and periapical periodontitis critically impairs dental pulp stem cells (DPSCs) functionality, yet the underlying molecular mechanisms remain poorly defined. This study aimed to investigate the effects of <em>Porphyromonas gingivalis</em> lipopolysaccharide (<em>P. gingivalis</em> LPS) on human DPSCs behavior and elucidate the transcriptomic changes underlying LPS-induced inflammation.</div></div><div><h3>Design</h3><div>Human DPSCs were exposed to 1 µg/mL LPS for 24 hours to establish an inflammatory model, with subsequent evaluation of proliferation (CCK-8), migration (Transwell), and odontogenic differentiation (ALP staining, mineralization assays and odontoblast markers expression). Cell cycle progression was quantified through flow cytometry, while RNA sequencing analysis delineated transcriptional alterations.</div></div><div><h3>Results</h3><div>LPS exposure significantly attenuated DPSCs proliferative capacity (<em>P</em> &lt; 0.01), suppressed migration (<em>P</em> &lt; 0.01), and impaired odontogenic differentiation, evidenced by diminished ALP activity, mineralized nodule formation and odontoblast markers expression. Cell cycle analysis revealed G0/G1 phase arrest (<em>P</em> &lt; 0.01), indicating proliferative quiescence. Transcriptomic profiling identified 467 differentially expressed genes. <em>P. gingivalis</em> LPS exerts its effects on DPSCs by concurrent activation of both canonical (Toll-like receptors, TLRs) and non-canonical (Nucleotide-binding oligomerization domain-like receptor) signaling pathway. Pathway enrichment revealed the enrichment of the several important signaling pathways such as Phosphatidylinositol 3 kinase-protein kinase B (PI3K-Akt), tumor necrosis factor (TNF) /Nuclear factor kappa B (NF-κB) and interleukin (IL) −17 signaling under LPS stimulation.</div></div><div><h3>Conclusion</h3><div><em>P. gingivalis</em> LPS disrupts DPSCs regenerative functions by modulating inflammatory and developmental signaling pathways, providing mechanistic insights into impaired pulp repair during oral infections.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106295"},"PeriodicalIF":2.2,"publicationDate":"2025-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144072046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of experimental polymer-containing solutions in preventing caries-like lesions on enamel","authors":"Raissa Manoel Garcia ,&nbsp;Astrid Carolina Valdivia Tapia ,&nbsp;Alessandra Buehler Borges ,&nbsp;George J. Eckert ,&nbsp;Frank Lippert ,&nbsp;Tais Scaramucci Forlin ,&nbsp;Anderson T. Hara","doi":"10.1016/j.archoralbio.2025.106286","DOIUrl":"10.1016/j.archoralbio.2025.106286","url":null,"abstract":"<div><h3>Objectives</h3><div>To evaluate experimental solutions containing polymers (Chitosan: CHI, Sodium linear polyphosphate: LPP, Polyacrylic acid: PAA; and 2-methacryloyloxyethyl phosphorylcholine: MPC) isolated, associated or not with fluoride (F), stannous ions (Sn), or F+Sn <em>in vitro</em> against caries-like lesion in enamel.</div></div><div><h3>Design</h3><div>Bovine enamel sections were randomly allocated into 20 groups (n = 14): Distilled water (DIW, negative control), F: 220 ppm F^-, Sn: 800 ppm Sn²⁺, F+Sn, and four polymers (0.5 % CHI, 2 % LPP, 0.1 % PAA, 2 % MPC) associated or not to F, Sn, or F+Sn. The single sections were individually subjected to a pH cycling: demineralization (3 h, 2 ×/d), experimental solution (1 min, 2 ×/d), and remineralization (overnight), for 5d. The enamel sections were analyzed using digital transverse microradiography (integrated mineral loss-ΔZ, and lesion depth-L). Data were analyzed using Kruskal-Wallis and Wilcoxon rank sum tests, with alpha= 0.05.</div></div><div><h3>Results</h3><div>When tested in isolation, LPP was the only polymer affording greater protection than DIW, while PAA enhanced ΔZ [median (Q1-Q3); vol%min× µm: 2530 (2070–3140) vs. 3710 (3410–3860) vs. 4375 (3820–4990) respectively; all p &lt; 0.05]. None of the polymers could increase the protection provided by F [1430 (1340,1570), p = 0.084] or F+Sn [1515(1150–1940), p = 0.182)]. CHI [2525(1890–3210), p = 0.01] and LPP [3105 (2460–3250), p = 0.006] increase the protection offered by Sn [3500 (3350–3790)], but PAA enhanced ΔZ [4340 (3540–4820), p = 0.044]. For L data, there are no polymer effects compared to DIW [93.30(82.60–104.80), p = 0.177], F [78.25(70.40–90.30), p = 0.340] or F+Sn [73.05(54.60–81.00), p = 0.056]. Only MPC decrease the degree effect of Sn [113.10 (110.40–124.30) and 99.20(92.60, 105.80), p = 0.003].</div></div><div><h3>Conclusions</h3><div>LPP was the only polymer that protected enamel against demineralization when tested in isolation. However, none of the polymers could increase the protection offered by fluoride or fluoride plus stannous ions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106286"},"PeriodicalIF":2.2,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143943752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}