Chondroprotective role of histone deacetylase 4 in early-stage of temporomandibular joint osteoarthritis in male rats

IF 2.1 4区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Archives of oral biology Pub Date : 2025-05-21 DOI:10.1016/j.archoralbio.2025.106301

Qi Ning , Weihua Han , Juanhong Meng , Yehua Gan

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引用次数: 0

Abstract

Objective

The aim of this study was to investigate the expression and role of histone deacetylase 4 (Hdac4) in temporomandibular joint osteoarthritis in rats.

Design

A model of temporomandibular joint (TMJ) osteoarthritis was established by partial discectomy in male rats. In the early stage of TMJ osteoarthritis, small interfering-HDAC4 (si-HDAC4) with adeno-associated virus was injected into the rat articular cavity to knockdown the expression of Hdac4 in TMJ. And an adenoviral vector was used to transfer Hdac4 into the rat articular cavity to increase the level of Hdac4 in TMJ. Subsequently, the pathological changes and stages of condylar cartilage were examined histopathologically at different time points after injection respectively, and the microstructure of TMJ subchondral bone was evaluated using micro computed tomography.

Results

Hdac4 expression in condylar cartilage was upregulated in the early-stage of TMJ osteoarthritis, but decreased in the mid to late-stage. Knockdown of Hdac4 expression in the early-stage of TMJ osteoarthritis accelerated the destruction of condylar cartilage in vivo, while overexpression of Hdac4 reduced the destruction of cartilage in the late-stage. Meanwhile, on micro computed tomography, the microstructure of subchondral bone in TMJ showed that overexpression of Hdac4 led to a decrease in incidence of bone sclerosis.

Conclusions

Our results suggested that Hdac4 has a certain chondroprotective effect in the development of TMJ osteoarthritis.

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组蛋白去乙酰化酶4在早期雄性大鼠颞下颌关节骨性关节炎中的软骨保护作用

目的探讨组蛋白去乙酰化酶4 （Hdac4）在大鼠颞下颌关节骨性关节炎中的表达及其作用。设计采用部分椎间盘切除术建立雄性大鼠颞下颌关节（TMJ）骨关节炎模型。在TMJ骨关节炎早期，将腺相关病毒小干扰- Hdac4 （si-HDAC4）注入大鼠关节腔，抑制Hdac4在TMJ中的表达。利用腺病毒载体将Hdac4转染大鼠关节腔，提高TMJ中Hdac4的表达水平。随后，分别在注射后不同时间点对髁突软骨的病理变化和分期进行组织病理学检查，并利用显微计算机断层扫描评估TMJ软骨下骨的微观结构。结果shdac4在髁突软骨中的表达在TMJ骨关节炎早期呈升高趋势，中晚期呈下降趋势。在TMJ骨关节炎早期，Hdac4表达下调可加速体内髁突软骨的破坏，而在晚期，Hdac4过表达可减缓软骨的破坏。同时，TMJ软骨下骨的显微计算机断层扫描显示，Hdac4过表达导致骨硬化发生率降低。结论Hdac4在TMJ骨关节炎的发生发展中具有一定的软骨保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Archives of oral biology 医学-牙科与口腔外科

CiteScore

5.10

自引率

3.30%

发文量

177

审稿时长

26 days

期刊介绍： Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry