Archives of oral biology最新文献

{"title":"Evergrowing incisors of diprotodont marsupials record age and life history","authors":"William M.G. Parker ,&nbsp;Justin W. Adams ,&nbsp;Eliza J. Campbell ,&nbsp;Graeme Coulson ,&nbsp;Gordon D. Sanson ,&nbsp;Alistair R. Evans","doi":"10.1016/j.archoralbio.2024.106018","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106018","url":null,"abstract":"<div><h3>Objective</h3><p>Tooth growth and wear are commonly used tools for determining the age of mammals. The most speciose order of marsupials, Diprotodontia, is characterised by a pair of procumbent incisors within the lower jaw. This study examines the growth and wear of these incisors to understand their relationship with age and sex.</p></div><div><h3>Design</h3><p>Measurements of mandibular incisor crown and root length were made for two sister species of macropodid (kangaroos and wallabies); <em>Macropus giganteus</em> and <em>Macropus fuliginosus</em>. Histological analysis examined patterns of dentine and cementum deposition within these teeth. Broader generalisability within Diprotodontia was tested using dentally reduced <em>Tarsipes rostratus</em> – a species disparate in body size and incisor function to the studied macropodids.</p></div><div><h3>Results</h3><p>In the macropodid sample it is demonstrated that the hypsodont nature of these incisors makes measurements of their growth (root length) and wear (crown length) accurate indicators of age and sex. Model fitting finds that root growth proceeds according to a logarithmic function across the lifespan, while crown wear follows a pattern of exponential reduction for both macropodid species. Histological results find that secondary dentine deposition and cementum layering are further indicators of age. Incisor measurements are shown to correlate with age in the sample of <em>T. rostratus</em>.</p></div><div><h3>Conclusions</h3><p>The diprotodontian incisor is a useful tool for examining chronological age and sex, both morphologically and microstructurally. This finding has implications for population ecology, palaeontology and marsupial evolution.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001390/pdfft?md5=c7987e80eea2ddcab22c393e4aa3cbae&pid=1-s2.0-S0003996924001390-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141308215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dental disease in a 15th-17th centuries skeletal sample of enslaved Africans (Lagos, Portugal)","authors":"Sofia N. Wasterlain ,&nbsp;Ana I. Rufino ,&nbsp;Maria Teresa Ferreira","doi":"10.1016/j.archoralbio.2024.106015","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106015","url":null,"abstract":"<div><h3>Objective</h3><p>Dental disease is frequently used as a proxy for diet and overall health of individuals of past populations. The aim of this study is to investigate dental disease in a sample of enslaved African individuals recovered from an urban dump (15th-17th centuries) in Lagos, Portugal.</p></div><div><h3>Design</h3><p>In all, 81 African individuals (&gt;12 years old) were analysed (19 males, 49 females, and 13 of unknown sex), in a total of 2283 alveoli, 2061 teeth, and 2213 interdental septa. Analysed oral pathologies include dental caries, periodontal disease, and ante-mortem tooth loss. Dental wear was also recorded.</p></div><div><h3>Results</h3><p>Dental caries affected 52.0 % of the teeth, although only 31.9 % were cavitated lesions. In all, 96.3 % of the individuals presented at least one cariogenic lesion. Gingivitis and periodontitis were recorded in 56.7 % and 19.0 % of the septa, respectively. Only one male individual had all septal areas healthy. Ante-mortem tooth loss was recorded in 38.3 % of the individuals, in a total of 96 teeth lost (4.2 %). Regarding occlusal wear, 70.8 % of the surfaces were recorded with grades 1–3.</p></div><div><h3>Conclusions</h3><p>The frequencies of the oral pathological conditions observed may not only reflect a cariogenic diet (rich in starches and with a high frequency of meals) but also the conditions during the maritime voyage of the first victims of the North Atlantic slave trade (xerostomia due to lack of water, sea sickness and vomiting, vitamin C deficiency, poor hygiene), and also the impact intentional dental modifications had on the dentitions.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001365/pdfft?md5=0249c33aa4bf662005953e576043a245&pid=1-s2.0-S0003996924001365-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249698","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intermittent compressive force regulates matrix metalloproteinases and tissue inhibitors of metalloproteinases expression in human periodontal ligament cells","authors":"Novena Dameria Pakpahan ,&nbsp;Maythwe Kyawsoewin ,&nbsp;Jeeranan Manokawinchoke ,&nbsp;Worachat Namangkalakul ,&nbsp;Chutimon Termkwancharoen ,&nbsp;Hiroshi Egusa ,&nbsp;Phoonsuk Limraksasin ,&nbsp;Thanaphum Osathanon","doi":"10.1016/j.archoralbio.2024.106011","DOIUrl":"10.1016/j.archoralbio.2024.106011","url":null,"abstract":"<div><h3>Objective</h3><p>This study aims to evaluate the effects of intermittent compressive force (ICF) on the expression of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) by human periodontal ligament cells (hPDLCs).</p></div><div><h3>Design</h3><p>hPDLCs were subjected to ICF with a magnitude of 1.5 g/cm² and loaded for 24 h. mRNA and protein expression of several MMPs and TIMPs were assessed using RT-PCR and ELISA analyses. An inhibitor of TGF-β (SB431542) was used to assess a possible role of TGF-β in the expression of MMPs and TIMPs under ICF.</p></div><div><h3>Results</h3><p>mRNA and protein analyses showed that ICF significantly induced expression of TIMP1 and TIMP3, but decreased expression of MMP1. Incubation with the TGF-β inhibitor and applied to ICF showed a downregulation of TIMP3, but expression of MMP1 was not affected.</p></div><div><h3>Conclusion</h3><p>ICF is likely to affect ECM homeostasis by hPDLCs by regulating the expression of MMP1 and TIMPs. Moreover, TGF-β1 regulated expression of TIMP3. These findings suggest ICF may decrease the degradation of ECM and may thus be essential for maintaining PDL homeostasis.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141181597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary exosomal microRNA profile as biomonitoring tool for diagnosis and prognosis of patients with head and neck squamous cell carcinoma: a systematic review","authors":"Lorenzo Sanesi ,&nbsp;Giorgio Mori ,&nbsp;Giuseppe Troiano ,&nbsp;Andrea Ballini ,&nbsp;Felice Valzano ,&nbsp;Mario Dioguardi ,&nbsp;Lorenzo Lo Muzio ,&nbsp;Marco Magalhaes ,&nbsp;Vito Carlo Alberto Caponio","doi":"10.1016/j.archoralbio.2024.106012","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106012","url":null,"abstract":"<div><h3>Objective</h3><p>Exosomes are extracellular vesicles found in saliva and other body fluids. These vesicles range in size from 30 to 150 nm and play a crucial role in intercellular communication, transporting different biomolecules, actively targeting cells. These vesicles regulate both physiological and pathological processes within recipient cells. MicroRNAs (miRs) are transported within exosomes and are delivered to target cells where they influence signaling pathways, taking on a crucial regulatory role in oncogenesis; for example, they are implicated in progression and infiltration of various cancers, such as head and neck squamous cell carcinoma (HNSCC).</p></div><div><h3>Material and methods</h3><p>A systematic literature search based on specific keywords, according to the PRISMA guidelines, was carried out on PubMed, Web of Science, Scopus, and Google Scholar. Only original articles were selected during this review. The risk of bias was assessed by QUADAS-2.</p></div><div><h3>Results</h3><p>At the end of the selection process 9 articles were included. In these studies, 41 miRs showed differential expression between healthy subjects and patient with HNSCC. The techniques varied among studies for the extraction and analysis of exosomal miRs. We presented also salivary exosomal miRs pathways, to give insights about pathogenetic mechanisms.</p></div><div><h3>Conclusions</h3><p>Exosomal microRNA are promising biomarkers for HNSCC detection. MiR-10b-5p, miR-486–5p, miR-24–3p, miR-412–3p, and miR-512–3p are the most promising markers applicable to diagnostics, while miR-1307–5p and miR-519c–3p resulted overexpressed and correlated to worse survival outcomes.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S000399692400133X/pdfft?md5=dcd09c385d87d5850ede0c4443fd3029&pid=1-s2.0-S000399692400133X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141328605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of cariogenic biofilms by using a swept-source optical coherence tomography in vitro","authors":"Tomoko Tabata ,&nbsp;Hisaichi Nakagawa ,&nbsp;Khairul Matin ,&nbsp;Masayuki Otsuki ,&nbsp;Akira Aoki ,&nbsp;Yasunori Sumi ,&nbsp;Yasushi Shimada","doi":"10.1016/j.archoralbio.2024.106009","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106009","url":null,"abstract":"<div><h3>Objective</h3><p>The objective was to measure the thickness of <em>Streptococcus mutans</em> (<em>S. mutans</em>) biofilms forming in an oral biofilm reactor (OBR) by using a noninvasive swept-source optical coherence tomography (SS-OCT) system at every 4 h time interval until 20 h and analyze the correlations with the amounts of biofilms.</p></div><div><h3>Methods</h3><p><em>S. mutans</em> biofilms were formed on square-shaped bovine enamel blocks inside an OBR. Biofilms were analyzed at every 4 h stage (4 h, 8 h, 12 h, 16 h and 20 h) using a SS-OCT system and a laser scanning confocal microscope (LSCM). The amounts of biofilms were measured at each stage by separating the water insoluble glucan (WIG) and bacterial cells. Co-relationships between the SS-OCT measured biofilm thickness and the amounts of adhered biofilms were analyzed.</p></div><div><h3>Results</h3><p>The thickness of biofilms detected on SS-OCT images at 4 h stage was 0.059 ± 0.029 (Av ± SD) mm which increased time-dependently in a linear fashion after 8 h stage and reached to 0.435 ± 0.159 mm at 20 h stage and the correlation coefficient was about 0.89. The amounts of biofilms; bacterial optical density (OD) and WIG concentration increased time-dependently were 0.035 ± 0.008 / mm² and 10.328 ± 2.492 µg/ mm² respectively at 20 h stage. Correlation coefficients of 0.66 between ‘the amounts of bacteria’ and ‘biofilm thickness on OCT’ and 0.67 between ‘the amounts of WIG’ and ‘biofilm thickness on OCT’ were obtained, suggesting that there was a relatively positive correlation between them.</p></div><div><h3>Conclusion</h3><p>The SS-OCT can be a useful tool to measure time-dependent growth of biofilms. Further studies are needed in order to assess biofilms using SS-OCT more accurately.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141249699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A finite element analysis of periodontal ligament fluid mechanics response to occlusal loading based on hydro-mechanical coupling model","authors":"Zhongyu Wang ,&nbsp;Sa Du ,&nbsp;Huilin Zhu ,&nbsp;Ke Yi ,&nbsp;Zhihui Tang ,&nbsp;Qing Li","doi":"10.1016/j.archoralbio.2024.106008","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106008","url":null,"abstract":"<div><h3>Objective</h3><p>Considering fluid stimulation is one of the essential biomechanical signals for periodontal tissues, this study aims to characterizing fluid mechanics response during occlusal loading by a hydro-mechanical coupling model for periodontal ligament.</p></div><div><h3>Design</h3><p>Models simulating periodontium with normal bone height and with intraosseous defects were built with three mechanical modules: tooth, periodontal ligament and alveolar bone. Tooth was modeled as linear elastic, and periodontal ligament and alveolar bone as a hydro-mechanical coupling model. Transient analyses under dynamic occlusal loading were performed. Fluid dynamics within periodontal ligament space was simulated and visualized by post-processing module.</p></div><div><h3>Results</h3><p>Reciprocating oscillatory flow occurred within the periodontal ligament under occlusal loading. Higher pore pressure and fluid velocity were observed in furcation and apical regions compared to mid-root and cervical regions. Intraosseous defects increased pore pressure and fluid velocity within the periodontal ligament, most significantly near the defect.</p></div><div><h3>Conclusion</h3><p>Based on the results of the hydro-mechanical coupling model, significant oscillatory fluid motion is observed within the periodontal ligament under occlusal loading. Particularly, higher fluid velocity is evident in the furcation and apical areas. Additionally, Intraosseous defects significantly enhance fluid motion within the periodontal ligament.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141083151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Locally injected bone marrow-derived mesenchymal stem cells reverts the histopathological changes in the tongue of carbimazole-induced hypothyroidism of male rats","authors":"Hebatallah Mahmoud ,&nbsp;Mohamed Badawy ,&nbsp;Sahar Abdel-Nasser Mohammed ,&nbsp;Maha El Shahawy","doi":"10.1016/j.archoralbio.2024.106010","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106010","url":null,"abstract":"<div><h3>Objective</h3><p>To decipher the role of locally injected bone marrow mesenchymal stem cells (BM-MSCs) in the tongue of hypothyroid rats.</p></div><div><h3>Design</h3><p>A total 24 male Wister rats were utilized and allocated into 3 groups (n = 8). As for the control group, rats received distilled water via oral gavage. In the hypothyroid group, rats administered carbimazole 5 mg/ 250 g/ day for 6 successive weeks, for hypothyroidism induction. The BM-MSC treated hypothyroid group (BM-MSC group); hypothyroid rats received local injection of 0.5 million BM-MSCs in tongue. Six weeks after BM-MSC injection, tongue samples were processed for Hematoxylin and eosin (H and E) staining, Ki67-immunohistochemistry and histomorphometric analysis.</p></div><div><h3>Results</h3><p>The hypothyroid group revealed degenerative alterations in the lingual papillae, and apparent thinning of the inferior lingual epithelium compared to their controls. Tongues of the BM-MSC group depicted restoration of the normal tongue histology. The Ki67 immunoreaction was apparently decreased in the lingual epithelium of hypothyroid group compared to their controls, however the BM-MSC group regained Ki67 immunostaining.</p></div><div><h3>Conclusion</h3><p>Our data suggest that administration of BM-MSCs rescued the degenerative changes in the lingual mucosa and one of the possible underlying mechanisms could be the restoration of cellular proliferation in the lingual epithelium.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141096177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral Candida albicans strain diversity and maintenance in HIV positive women in South Africa","authors":"FJ Owotade ,&nbsp;Z. Gulube ,&nbsp;M. Patel","doi":"10.1016/j.archoralbio.2024.106007","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.106007","url":null,"abstract":"<div><h3>Objective</h3><p>This study investigated <em>C. albicans</em> strain diversity and maintenance in the oral cavity of HIV positive women over a 6 month period.</p></div><div><h3>Study design</h3><p><em>C. albicans</em> strains were isolated from 17 HIV positive women at Charlotte Maxeke Academic Hospital, Johannesburg at 3 intervals over a 6 month period. Strains were genotyped using ABC and Multilocus Sequence Typing (MLST) techniques. In the MLST technique, for each strain, a Diploid Sequence Type (DST) number was obtained. Using cluster analysis, an Unweighted Pair Group Method with Arithmetic Mean (UPGMA) dendrogram and a matrix of strain similarities were generated. Strains were also compared to the previous South African isolates documented in the MLST database.</p></div><div><h3>Results</h3><p>Ninety four percent of women carried the same ABC genotype for 6 months. MLST technique, showed that ten women (58.8%) carried the same DST at 2 visits, while seven (41.2%) carried different DST at all visits. Further analysis showed that 64.7% of women were recolonised with different strains and 35.3% carried the same strains of <em>C. albicans</em> with heterozygosity. A total of 40 diploid sequence types were identified of which 27 DSTs were unique to this study group that were added to the MLST database. Most of the strains were closely related to previously isolated strains from South Africa.</p></div><div><h3>Conclusion</h3><p>Recolonization of the oral cavity with different strains and microevolution of the original strains of <em>C. albicans</em> can occur, which can be a potential problem for HIV patients, in whom highly virulent and drug resistant strains can emerge.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001286/pdfft?md5=76a78bbc42293d054758bb37ab985934&pid=1-s2.0-S0003996924001286-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141090364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are deciduous upper molars and lower canines useful for sex estimation?","authors":"Cármen Valente ,&nbsp;Alexandra Teixeira ,&nbsp;Pedro Oliveira ,&nbsp;Inês Morais Caldas","doi":"10.1016/j.archoralbio.2024.106006","DOIUrl":"10.1016/j.archoralbio.2024.106006","url":null,"abstract":"<div><h3>Objective</h3><p>This paper investigates whether deciduous upper molars and lower canines have sexual dimorphic features, exploring these teeth' dimensions and the presence of Zuckerkandl’s tubercle and Carabelli’s cusp on the first and second upper molars.</p></div><div><h3>Design</h3><p>We analyzed 64 pairs of dental plaster casts from 34 females and 30 males aged between 3 and 12 years. We measured the first and second deciduous upper molars and the lower deciduous canines (maximum mesiodistal and buccolingual length), and we registered the presence of the Zuckerkandl’s tubercle and the Carabelli’s cusp on the first and second upper molars, respectively.</p></div><div><h3>Results</h3><p>Regarding the differentiation between sexes using Carabelli’s cusp and Zuckerkandl’s tubercle, the classification was not independent of Carabelli’s cusp presence only for tooth 65 (p = 0.035). In all other teeth, whether for Carabelli’s cusp or Zuckerkandl’s tubercle, their presence was similar for both sexes. There were statistically significant differences between sexes (p &lt; 0.05) for the buccolingual measurements of both upper second molars, the first right upper molar, and the right canine. The developed model allowed for a 64.1% accuracy in sex estimation.</p></div><div><h3>Conclusions</h3><p>The study suggests that while Carabelli’s cusp and Zuckerkandl’s tubercle in upper deciduous molars don't consistently differ between sexes, tooth size, particularly the buccolingual measurements of certain teeth, including upper deciduous molars and lower canines, may provide a more reliable criterion for sex estimation. The developed model depicted moderate accuracy, underscoring the need for a multifactorial approach when estimating sex from skeletal remains. It suggests that while dental features can contribute to sex estimation, they should be used in conjunction with other skeletal or molecular markers to improve accuracy.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001274/pdfft?md5=a864d254be7f45701dcd8724b08cc621&pid=1-s2.0-S0003996924001274-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141025447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High expression of serine protease inhibitor kazal type 1 predicts poor prognosis and promotes the progression and invasion of oral tongue squamous cell carcinoma","authors":"Shuang Wang ,&nbsp;Yaping Sun ,&nbsp;Dan Shao ,&nbsp;Yunjie Pan ,&nbsp;Xiaoyan Gao ,&nbsp;Peng Zhao ,&nbsp;Qiaoling Liu ,&nbsp;Gaishuang Shang ,&nbsp;Wei Shang ,&nbsp;Zhiguang Fu ,&nbsp;Yong Sun","doi":"10.1016/j.archoralbio.2024.106003","DOIUrl":"10.1016/j.archoralbio.2024.106003","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to investigate the expression of serine protease inhibitor kazal type 1 (SPINK1) and its carcinogenic effect in oral tongue squamous cell carcinoma (OTSCC). Design: Initially, bioinformatics analysis was conducted using data from The Cancer Genome Atlas and Gene Expression Omnibus to compare SPINK1 mRNA expression between malignant and adjacent tissues. Subsequently, the impact of differential expression on survival and other clinical variables was examined. Additionally, histology microarray analysis was performed to assess SPINK1 protein expression in 35 cases of malignant and adjacent tissues. Finally, alterations in SPINK1 expression were evaluated to determine its biological phenotypes in OTSCC, including proliferation, apoptosis, invasion, and metastasis. Results: OTSCC tissues exhibit higher levels of SPINK1 compared to surrounding cancerous tissues. Notably, increased SPINK1 expression correlates with the pathological N stage and independently predicts overall survival among patients with OTSCC. Conclusion: Suppression of SPINK1 inhibited OTSCC cell proliferation, invasion, and motility while promoting apoptosis. These findings suggest that SPINK1 may serve as a prognostic biomarker as well as a potential therapeutic target for managing OTSCC.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001249/pdfft?md5=5695cfa5b83ee739e7b8ca03058410c8&pid=1-s2.0-S0003996924001249-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141034902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}