Archives of oral biology最新文献

{"title":"Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis","authors":"","doi":"10.1016/j.archoralbio.2024.106066","DOIUrl":"10.1016/j.archoralbio.2024.106066","url":null,"abstract":"<div><h3>Objective</h3><p>This study investigated the clinical importance of long noncoding RNA myocardial infarction-associated transcript (MIAT) in periodontitis and its impact on the functional regulation of human periodontal ligament fibroblasts (hPDLFs).</p></div><div><h3>Methods</h3><p>Ninety-eight periodontitis patients and 74 healthy controls were enrolled. In vitro cellular models were created using <em>Porphyromonas gingivalis</em> lipopolysaccharide (Pg-LPS) to stimulate hPDLFs. Real-time quantitative polymerase chain reaction was used to measure mRNA levels of MIAT and osteogenic factors. Inflammation factor concentration was assessed using an enzyme-linked immunosorbent assay. Cell viability and apoptosis were examined by cell counting kit −8 and flow cytometry assay. The targeting relationship was verified by the dual-luciferase reporter and RNA Immunoprecipitation assay.</p></div><div><h3>Results</h3><p>Highly expressed MIAT and Dicckopf-1 (DDK1), and lowly expressed miR-204–5p were found in the gingival crevicular fluid of periodontitis patients and Pg-LPS induced hPDLFs. MIAT has a sensitivity of 76.53 % and a specificity of 86.49 % for identifying patients with periodontitis among healthy individuals. MIAT acts as a sponge for miR-204–5p and upregulates DDK1 mRNA expression. Silencing of MIAT diminished the promotion of apoptosis and inflammation in hPDLFs by Pg-LPS and enhanced osteogenic differentiation. However, a miR-204–5p inhibitor significantly reversed the effect of silenced MIAT.</p></div><div><h3>Conclusions</h3><p>MIAT may act as a promising biomarker for periodontitis. It modulates apoptosis, inflammation, and osteogenic differentiation of PDLFs by focusing on the miR-204–5p/DKK1 axis, indicating its potential as a new therapeutic target for treating periodontitis.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial effects of epigallocatechin-3-gallate, a catechin abundant in green tea, on periodontal disease-associated bacteria","authors":"","doi":"10.1016/j.archoralbio.2024.106063","DOIUrl":"10.1016/j.archoralbio.2024.106063","url":null,"abstract":"<div><h3>Objective</h3><p>Epigallocatechin-3-gallate (EGCG), a catechin abundant in green tea, exhibits antibacterial activity. In this study, the antimicrobial effects of EGCG on periodontal disease-associated bacteria (<em>Porphyromonas gingivalis</em>, <em>Prevotella intermedia</em>, <em>Prevotella nigrescens</em>, <em>Fusobacterium nucleatum</em>, and <em>Fusobacterium periodontium</em>) were evaluated and compared with its effects on <em>Streptococcus mutans</em>, a caries-associated bacterium.</p></div><div><h3>Results</h3><p>Treatment with 2 mg/ml EGCG for 4 h killed all periodontal disease-associated bacteria, whereas it only reduced the viable count of <em>S. mutans</em> by about 40 %. Regarding growth, the periodontal disease-associated bacteria were more susceptible to EGCG than <em>S. mutans</em>, based on the growth inhibition ring test<em>.</em> As for metabolism, the 50 % inhibitory concentration (IC₅₀) of EGCG for bacterial metabolic activity was lower for periodontal disease-associated bacteria (0.32–0.65 mg/ml) than for <em>S. mutans</em> (1.14 mg/ml). Furthermore, these IC₅₀ values were negatively correlated with the growth inhibition ring (r = −0.73 to −0.86). EGCG induced bacterial aggregation at the following concentrations: <em>P. gingivalis</em> (&gt;0.125 mg/ml), <em>F. periodonticum</em> (&gt;0.5 mg/ml), <em>F. nucleatum</em> (&gt;1 mg/ml), and <em>P. nigrescens</em> (&gt;2 mg/ml). <em>S. mutans</em> aggregated at an EGCG concentration of &gt; 1 mg/ml.</p></div><div><h3>Conclusion</h3><p>EGCG may help to prevent periodontal disease by killing bacteria, inhibiting bacterial growth by suppressing bacterial metabolic activity, and removing bacteria through aggregation.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001845/pdfft?md5=ad101c84b7d1199d7d35809514c558ee&pid=1-s2.0-S0003996924001845-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141918311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A persistent mineralization process in alveolar bone throughout the postnatal growth stage in rats","authors":"","doi":"10.1016/j.archoralbio.2024.106062","DOIUrl":"10.1016/j.archoralbio.2024.106062","url":null,"abstract":"<div><h3>Objective</h3><p>Alveolar bone quality is essential for the maxillofacial integrity and function, and depends on alveolar bone mineralization. This study aims to investigate the in vivo changes in alveolar bone mineralization, from the perspective of mineral deposition and crystal transition in postnatal rats.</p></div><div><h3>Design</h3><p>Nine postnatal time points of Wistar rats, ranging from day 1 to 56, were set to obtain the maxillary alveolar bone samples. Each time point consisted of ninety rats, with 45 females and 45 males. Macromorphology of alveolar bone was reconducted by Micro-Computed Tomography and the mineral content was quantified via Thermogravimetric analysis, Scanning Electron Microscope, High-Resolution Transmission Electron Microscopy and vibrational spectroscopy. Furthermore, the crystallinity and composition were characterized by vibrational spectroscopy, X-ray Diffraction, X-ray Photoelectron Spectroscopy and Selected Area Electron Diffraction.</p></div><div><h3>Results</h3><p>The progressive increase of mineral deposition was accompanied by substantial growth in alveolar bone mass and volume in postnatal rats. Whereas the mineral percentage initially decreased and then increased, reaching a nadir on postnatal day 14 (P14) when tooth eruption was first observed. Besides, localized mineralization was initiated by the formation of amorphous precursors and then converted into mineral crystals, while there was no statistically significant change in the average crystallinity of the bone during growth.</p></div><div><h3>Conclusion</h3><p>Mineralization of alveolar bone is ongoing throughout the early growth in postnatal rats. Mineral deposition increases with age, whereas the crystallinity remains stable within a certain range. Besides, the mineral percentage reaches its lowest point on P14, which may be attributed to tooth eruption.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141879897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neohesperidin exerts subtle yet comprehensive regulation of mouse dental papilla cell-23 in vitro","authors":"","doi":"10.1016/j.archoralbio.2024.106055","DOIUrl":"10.1016/j.archoralbio.2024.106055","url":null,"abstract":"<div><h3>Objective</h3><p>The molecular regulation of odontoblasts in dentin formation remains largely uncharacterized. Using neohesperidin (NEO), a well-documented osteoblast regulator, we investigated whether and how NEO participates in odontoblast regulation through longitudinal treatments using various doses of NEO.</p></div><div><h3>Design</h3><p>Mouse dental papilla cell-23 (MDPC-23) served as a model for odontoblasts. MDPC-23 were treated with various doses of NEO (0, 1, 5, 10, 15, 20 μmol/L). Proliferation was assessed using the Cell counting kit-8 assay. Survival/apoptosis was assayed by live/dead ratio. Migration capability was assessed using scratch healing and Transwell migration assays. Mineralization was assessed using alkaline phosphatase staining and alizarin red staining. The expression levels of four key genes (Runx2, osteocalcin [OCN], β-catenin, and bone morphogenetic protein [BMP]−2) representing NEO-induced differentiation of MDPC-23 were measured by quantitative reverse transcription polymerase chain reaction.</p></div><div><h3>Results</h3><p>The proliferation trajectories of MDPC-23 treated with the five doses of NEO demonstrated similar curves, with a rapid increase in the 10 μmol/L NEO condition after 48 h of treatment. Similar dose-dependent trajectories were observed for survival/apoptosis. All four key genes representing odontogenic differentiation were upregulated in MDPC-23 induced by NEO treatments at two optimal doses (5 μmol/L and 10 μmol/L). Optimal migration and mobility trajectories were observed in MDPC-23 treated with 10 μmol/L NEO. Optimal mineralization was observed in MDPC-23 treated with 5 μmol/L NEO.</p></div><div><h3>Conclusion</h3><p>NEO can subtly regulate odontoblast proliferation, differentiation, migration, and mineralization in vitro. NEO at 5–10 μmol/L offers a safe and effective perspective for clinical promotion of dentin bridge formation in teenagers.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Arecoline promotes fibroblast activation and M2-macrophage polarization by up-regulating the expression of IL-4","authors":"","doi":"10.1016/j.archoralbio.2024.106052","DOIUrl":"10.1016/j.archoralbio.2024.106052","url":null,"abstract":"<div><h3>Objective</h3><p>To determine the biological effects of arecoline on oral submucous fibrosis (OSF).</p></div><div><h3>Design</h3><p>The differential genes between OSF tissue and normal oral tissue were collected form GSE64216 dataset, analyzed by Gene Expression Omnibus (GEO) database. Real-time PCR and immunohistochemistry were used to analyze the expression of IL-4 gene and protein in oral tissue. Enzyme-linked immunosorbent assay (ELISA) was used to analyze the expression of exocrine IL-4 protein in human oral fibroblasts (HOF) pre-treated by arecoline. Cell Counting Kit-8 (CCK-8) and transwell assays were used to analyze the proliferation and migration of HOF cells, respectively. After IL-4 was knocked down by short hairpin (sh) plasmid, the proliferation and migration of HOF cells were detected. Flow cytometry was used to analyze the proportion of M2-macrophages. Real-time PCR and immunohistochemistry were used to verify the expression of biomarker proteins of macrophages in OSF tissues.</p></div><div><h3>Results</h3><p>The expression of IL-4 gene and protein were both up-regulated in OSF tissue. Arecoline could enhance the expression of IL-4 gene and exocrine protein in HOF cells, and promote the proliferation and migration of HOF cells. While knockdown of IL-4 could inhibit arecoline-induced proliferation and migration in HOF cells. The results of flow cytometry showed that recombinant human IL-4 (rhIL-4) protein could increase the proportion of M2-macrophages. Similarly, the results of real-time PCR and immunohistochemistry showed the expression of ARG1 (Biomarker proteins of M2-macrophage) was up-regulated in OSF tissues.</p></div><div><h3>Conclusion</h3><p>Arecoline promotes activation of fibroblasts and polarization of M2-macrophages by up-regulating the expression of IL-4.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141763188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel missense variant in CAT gene causing acatalasemia with gangrenous periodontitis (Takahara’s disease)","authors":"","doi":"10.1016/j.archoralbio.2024.106054","DOIUrl":"10.1016/j.archoralbio.2024.106054","url":null,"abstract":"<div><h3>Objectives</h3><p>Acatalasemia is a very rare disorder characterized by gangrenous oral ulcerations and is caused by biallelic variants in the <em>CAT</em> gene which encodes the catalase enzyme that decomposes the hydrogen peroxide molecules to remove their toxic effect. We report two siblings from a consanguineous Egyptian family presenting with joint hyperlaxity, loose dentitions with gangrenous periodontitis, and early loss of teeth.</p></div><div><h3>Study design</h3><p>The patients were clinically suspected to have the periodontal type of Ehlers-Danlos syndrome and thus genetic testing of <em>C1S</em> and <em>C1R</em> causative genes was carried out first by Sanger sequencing then exome sequencing (ES) was considered.</p></div><div><h3>Results</h3><p>No pathogenic variants were detected in <em>C1S</em> and <em>C1R</em> genes then ES revealed a new homozygous missense variant in the <em>CAT</em> gene segregating in the family, c .635 T &gt; G (p.Met212Arg).</p></div><div><h3>Conclusion</h3><p>We describe the first Egyptian cases with acatalasemia and expand the mutational spectrum of this rare disorder. Premature loss of teeth is an emerging finding in our cases and addresses the hazardous systemic manifestations associated with the disorder. The rarity of inherited orodental diseases renders the accurate diagnosis difficult and complicates the symptoms. Therefore, the use of advanced molecular technologies is highly advisable for early diagnosis and management of patients.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cerium- and samarium-nitrate interaction and accumulation on human dentin","authors":"","doi":"10.1016/j.archoralbio.2024.106053","DOIUrl":"10.1016/j.archoralbio.2024.106053","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the accumulation of cerium-nitrate and samarium-nitrate on dentin without or with smear-layer and to test their antibacterial activity.</p></div><div><h3>Design</h3><p>24 dentin-enamel slices were cut from 24 extracted molars. 12 slices underwent smear-layer creation (320 grit, 200 g, 5 s), the other 12 smear-layer removal (20 % EDTA, 300 s). Slices were halved to 48 semilunar-shaped specimens. One specimen per tooth was treated with either Ce(NO₃)₃ (50 wt% aqueous solution; pH = 1.29; n = 6) or Sm(NO₃)₃ (50 wt% aqueous solution; pH = 1.88; n = 6). The other specimen served as control (A. demin). After water rinsing, elemental composition (Ce, Sm, Ca, P, O, N, Na, Mg, C) was measured (EDX; EDAX Octane-Elect, APEX v2.5, low-vacuum) in dentin. Atomic percent (At%), Ca/P- and Ca/N-ratios were calculated and analyzed non-parametrically (α = 0.05, error rates method). Additionally, antibacterial activity (2 min exposure) of Ce(NO₃)₃ and Sm(NO₃)₃ against <em>Streptococcus mutans</em>, <em>Actinomyces naeslundii</em>, <em>Schaalia odontolytica</em>, and <em>Enterococcus faecalis</em> was determined (colony forming units) after anaerobic incubation at 37 °C for 24 h (control: 0.2 % CHX).</p></div><div><h3>Results</h3><p>At% (median) of Ce and Sm were as follows: Ce(NO₃)₃ 3.4 and 0.9 At%Ce with and without smear-layer, respectively; Sm(NO₃)₃ 2.4 and 1.3 At%Sm with and without smear-layer, respectively. Ce(NO₃)₃ and Sm(NO₃)₃-application significantly decreased Ca/P-ratios (1.22 – 1.45; p ≤ 0.02) compared to controls (1.47 – 1.63). With smear-layer, significantly higher Ca/N-ratios (5.1 – 29.3) could be detected across all groups (p ≤ 0.004) compared to specimens without smear-layer (0.37 – 0.48). Ce(NO₃)₃ and Sm(NO₃)₃ showed reduction rates of up to ≥ 5 log10 steps for <em>S. mutans</em>, <em>A. naeslundii</em>, and <em>S. odontolytica</em>.</p></div><div><h3>Conclusions</h3><p>Cerium and samarium nitrate showed accumulation on dentin and certain antibacterial activity and could therefore be identified as potential compounds to treat and prevent dentin and root caries and dentin hypersensitivity.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001742/pdfft?md5=eb8fc2330377af716b3c6a1a73fca4c3&pid=1-s2.0-S0003996924001742-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141715170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential impact of chronic intermittent hypoxia and stress changes on condylar development","authors":"","doi":"10.1016/j.archoralbio.2024.106051","DOIUrl":"10.1016/j.archoralbio.2024.106051","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to determine the effects of chronic intermittent hypoxia (CIH) and stress change (SC) on the development of the condyle in mouth breathing rats.</p></div><div><h3>Design</h3><p>A total of 120 4-week-old rats were randomly assigned to one of five groups. The control (Ctrl) group was the blank control and the intermittent nasal obstruction (INO) group was the positive control. Mild CIH (mCIH) and severe CIH (sCIH) groups were developed by adjusting environmental oxygen concentration and monitoring real-time blood oxygen saturation (SpO₂). The SC group was developed using INO, increased environmental oxygen concentration, and real-time SpO₂ monitoring. Six rats from each group were sacrificed for analysis at 0, 1, 2, or 4 weeks.</p></div><div><h3>Results</h3><p>Similar to the INO group, condyle and mandibular body development in the sCIH group, but not in the mCIH group, was significantly inhibited compared with the Ctrl group. The SC group had inhibited development of the condyle, especially of the posterior zone, but had minimal impact on the growth of the mandible.</p></div><div><h3>Conclusion</h3><p>The inhibitory effects of CIH on the development of the condyle and mandibular body were SpO₂-dose-dependent. When SC occurred, inhibited development was observed in the posterior zone of condyle but not the whole mandible. These findings provide important insights for targeted interventions that address the consequences of mouth breathing in children.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141697111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological characteristics of non-carious cervical lesions. A systematic review","authors":"","doi":"10.1016/j.archoralbio.2024.106050","DOIUrl":"10.1016/j.archoralbio.2024.106050","url":null,"abstract":"<div><h3>Objectives</h3><p>This systematic review assessed the morphological characteristics of non-carious cervical lesions (NCCL), among clinical and <em>ex-vivo</em> studies assessed by observational and imaging methods.</p></div><div><h3>Design</h3><p>The search strategy was conducted on four online databases (MEDLINE, Embase, Scopus and Web of Science) and grey literature (Google Scholar and OpenGrey). The study selection was performed by three reviewers from March to September (2023). The eligibility criteria were established according to the PICO strategy and included NCCL, morphological characteristics and clinical and <em>ex-vivo</em> study designs. The data extraction considered general data that identifies the study, evaluation method, parameter to assess the outcome and the main results for each study. The risk of bias was evaluated using Joanna Briggs Institute critical appraisal tool, and a personalized tool.</p></div><div><h3>Results</h3><p>The search resulted in 252 studies. A total of 14 studies were included. Prevalence of NCCLs ranged from 3.5 %to 77.78 % with a higher presence in premolars. Common characteristics were wear facets, occluded tubules or cracks, occlusal stress, scratch marks, dimples and craters, structure loss, and dentin sclerosis, which appear more often on buccal surface and were generally classified as wedge-shaped, saucer-shaped. Etiological hypothesis was mainly related to multifactorial factors. In most of the studies, the risk of bias was classified as high.</p></div><div><h3>Conclusions</h3><p>The morphological characteristics of NCCL showed a wide range of descriptions regarding appearance, prevalence, lesion-related measures, and macro and microscopic descriptions.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141689608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bone marrow derived mesenchymal stem cells restored GLUT1 expression in the submandibular salivary glands of ovariectomized rats","authors":"Menna Abdulqader El-Badawy ,&nbsp;Mohamed Badawy ,&nbsp;Maha El Shahawy","doi":"10.1016/j.archoralbio.2024.106048","DOIUrl":"10.1016/j.archoralbio.2024.106048","url":null,"abstract":"<div><h3>Objective</h3><p>Loss of ovarian function in menopause is commonly associated with salivary gland dysfunction. The aim is to study the possible therapeutic effect of bone marrow mesenchymal stem cells (BM-MSCs) on the altered structure of the submandibular salivary glands (SMGs) of ovariectomized rats.</p></div><div><h3>Design</h3><p>Twenty-four female, adult, Wistar rats were used and distributed into three groups (8 rats/group). The control group included sham-operated rats. The ovariectomized group consisted of rats with removed ovaries. The third group consisted of ovariectomized rats received injections, via tail, of MSCs extracted from bone marrow of 3-weeks-old rat hind limb (BM-MSC group). Four weeks after BM-MSC transplantation, the bone mineral density (BMD) of the femur was detected. The SMG was dissected and processed for histological, immunohistochemical, and histomorphometric analyses.</p></div><div><h3>Results</h3><p>The ovariectomized rats depicted low BMD in the femur. The SMG acini revealed atrophy. The ductal and acinar cells depicted vacuolization and abnormal nuclear histology. GLUT1 immunostaining was decreased in SMG ducts. The BM-MSC group resumed the normal SMG histology and GLUT1 immunolabelling.</p></div><div><h3>Conclusions</h3><p>BM-MSC therapy restored the normal SMG structure and GLUT1 immunostaining in the treated ovariectomized rats, suggesting improved glucose transporting function.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}