Archives of oral biology最新文献

{"title":"Effect of high-lipid environment on human gingival fibroblast on titanium surface: An in-vitro study","authors":"Shuyi Tan,&nbsp;Maoquan Li,&nbsp;Zhiqiang Liu,&nbsp;Aijie Chen,&nbsp;Jialing Wu,&nbsp;Ruiqi Li,&nbsp;Xiachen Li,&nbsp;Shuangquan Wan","doi":"10.1016/j.archoralbio.2025.106454","DOIUrl":"10.1016/j.archoralbio.2025.106454","url":null,"abstract":"<div><h3>Objectives</h3><div>Hyperlipidemia is associated with dental peri-implantitis. Long-term success of dental implant relies on good peri-implant soft tissue barrier. This study aims to investigate the effect of high-lipid environment on biological activities of gingival fibroblast on titanium surface so as to explore the effect of hyperlipidemia on peri-implant soft tissue.</div></div><div><h3>Design</h3><div>Human gingival fibroblast-1(HGF-1) was seeded on the titanium surfaces and cultured in normal and high-lipid culture medium for 4 days. Cell adhesion and proliferation were assessed by cell counting and morphological observation following fluorescent staining. RNA sequencing were performed afterwards, followed by differentially expressed genes analysis, several enrichment analyses and Protein-Protein Interaction (PPI) analysis.</div></div><div><h3>Results</h3><div>The number of HGF-1 cells significantly decreased and the morphology of HGF-1 changed in high-lipid culture environment. Moreover, the expression levels of several hub genes, including Interleukin-6 (IL-6), C-X-C motif chemokine ligand 8 (CXCL8), and Activating Transcription Factor 3 (ATF3), as well as the activity of pathways such as the IL-17 signaling pathway and nuclear factor kappa-B (NF-κB) signaling pathway, were elevated. Meanwhile, the down-regulation of cell proliferation activities, like DNA replication and cell division was also observed.</div></div><div><h3>Conclusions</h3><div>High-lipid environment impaired the adhesion and proliferation of HGF-1 on titanium surface, which may be associated with the upregulation of specific genes and pathways, alongside the downregulation of cell proliferation-related activities.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106454"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibition of protein kinase C activity enables mineralization of senescent dental follicle cells with almost no osteogenic differentiation potential","authors":"Christian Morsczeck,&nbsp;Anja Reck,&nbsp;Michela De Pellegrin,&nbsp;Torsten E. Reichert","doi":"10.1016/j.archoralbio.2025.106468","DOIUrl":"10.1016/j.archoralbio.2025.106468","url":null,"abstract":"<div><h3>Objective</h3><div>Dental follicle cell lines with a senescence phenotype have a poor differentiation potential into biomineralizing cells. Previous studies have shown that protein kinase C (PKC) and protein kinase B (AKT) regulate the differentiation of DFCs. This study investigates the extent to which regulation of PKC and AKT can improve the differentiation of dental follicle cells with poor osteogenic potential.</div></div><div><h3>Design</h3><div>Human senescence dental follicle cells with poor osteogenic differentiation potential were osteogenic differentiated with cell culture media containing dexamethasone or bone morphogenetic protein (BMP) 2 as an inducer. GÖ6976 was used as a PKC inhibitor, and MK-2206 as an AKT inhibitor. The AKT activator SC-79 was also used. Western blot analyses were performed with specific antibodies for the active form of AKT, phosphorylated substrate of PKC and collagen 1. Osteogenic differentiation was quantitatively determined by measuring alkaline phosphatase (ALP) activity and biomineralization using alizarin staining. The gene expression of sclerostin (SOST) and PTHLH was quantitatively determined using real-time RT-PCRs.</div></div><div><h3>Results</h3><div>The results showed that both the inhibitor MK-2206 inhibits AKT and the activator SC-79 can activate AKT in DFCs. Only inhibition of AKT slightly but significantly enhanced osteogenic differentiation. While inhibition of PKC activity apparently only occurred from day 14 of differentiation using the inhibitor GÖ6976, PKC inhibition promoted osteogenic differentiation and inhibits the expression of SOST and Parathyroid hormone-related protein (PTHLH).</div></div><div><h3>Conclusion</h3><div>Our results suggest that the addition of GÖ6976 is an efficient method to induce biomineralization in senescent DFCs.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106468"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of the correlativity of SOX4 and epithelial-mesenchymal transition markers expression in the prognosis of oral squamous cell carcinoma","authors":"Pengfei Zhao ,&nbsp;Xiaohu Lin ,&nbsp;Yuanyuan Cao ,&nbsp;Zhang Zhao ,&nbsp;Xiaoge Zhang ,&nbsp;Yang Zhao ,&nbsp;Songge Liu ,&nbsp;Bo Hu ,&nbsp;Wei Cao ,&nbsp;Wei Li ,&nbsp;Xianjun Zhang","doi":"10.1016/j.archoralbio.2025.106465","DOIUrl":"10.1016/j.archoralbio.2025.106465","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the prognostic significance of Sex-determining region Y-box 4 (SOX4) and epithelial–mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, TWIST1) in oral squamous cell carcinoma (OSCC) and to evaluate their functional role.</div></div><div><h3>Design</h3><div>A total of 250 OSCC tissues and 80 normal oral mucosa specimens were analyzed by immunohistochemistry for SOX4, E-cadherin, N-cadherin, and TWIST1 expression. Kaplan–Meier survival curves with log-rank tests, Cox proportional hazards regression (univariate and multivariate), and Spearman correlation were performed to assess prognostic value and clinicopathological associations. Functional validation was conducted by SOX4 knockdown and overexpression in OSCC cell lines, followed by migration, invasion, and EMT marker analyses.</div></div><div><h3>Results</h3><div>High SOX4, N-cadherin, and TWIST1 expression correlated with larger tumor size, lymph node metastasis, advanced clinical stage, and poor differentiation (p &lt; 0.05). SOX4 expression showed positive correlation with N-cadherin and TWIST1, and negative correlation with E-cadherin. Kaplan–Meier analysis demonstrated that high SOX4, N-cadherin, and TWIST1 expression were associated with significantly poorer overall survival. Functional assays confirmed that SOX4 overexpression promoted EMT and cell motility, whereas knockdown reversed these effects.</div></div><div><h3>Conclusions</h3><div>In summary, our study identified SOX4, N-cadherin, TWIST1, E-cadherin, lymph node metastasis, and clinical staging as independent factors influencing the prognosis of OSCC. SOX4 promotes EMT, thereby influencing the progression and prognosis of OSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106465"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growing new teeth: A systematic review of functional whole-tooth regeneration in orthotopic animal models","authors":"Pei-Jung Wu,&nbsp;Mar Jovani-Sancho","doi":"10.1016/j.archoralbio.2025.106461","DOIUrl":"10.1016/j.archoralbio.2025.106461","url":null,"abstract":"<div><h3>Objective</h3><div>To identify and evaluate current strategies for functional whole-tooth regeneration in orthotopic animal models with high translational potential.</div></div><div><h3>Design</h3><div>A systematic review of <em>in vivo</em> studies involving orthotopic implantation of generated tooth constructs, analyzing experimental design, cell sources, scaffolding materials, implantation techniques, and outcome measures including histological, radiological, and functional evaluations.</div></div><div><h3>Results</h3><div>Considerable heterogeneity was observed in animal models, developmental stages at implantation, and cellular components. Polycaprolactone (PCL) membranes were often associated with improved eruption and reduced ankylosis, suggesting enhanced periodontal integration.</div></div><div><h3>Conclusions</h3><div>PCL-based scaffolding systems may facilitate functional tooth regeneration, but further standardized <em>in vivo</em> research is needed to validate translational potential.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106461"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145566602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Third-molar agenesis shifts mandibular second-molar mineralisation timeline: An orthopantomographic study in South Indian children","authors":"Subramanyeswara Swamy Chinni ,&nbsp;Nallan CSK Chaitanya ,&nbsp;Waheeda Shahnaz ,&nbsp;Usha Gangavarapu ,&nbsp;Shivani Ramesh Mungala ,&nbsp;Sudheer B. Balla","doi":"10.1016/j.archoralbio.2025.106466","DOIUrl":"10.1016/j.archoralbio.2025.106466","url":null,"abstract":"<div><h3>Background and objective</h3><div>In many jurisdictions, the 14-year threshold is a legally significant factor in determining criminal responsibility and related decisions. We investigated whether third-molar agenesis is associated with differences in the mineralisation stage of the mandibular second molar in South Indian children.</div></div><div><h3>Design</h3><div>Orthopantomograms from 570 children (240 males, 330 females; 10–14.99 years) were staged using Demirjian A to H criteria. Descriptive comparisons were supplemented with a proportional-odds ordinal logistic regression, modelling second-molar stage as the ordered outcome, with agenesis extent, age, and sex as predictors. Results are reported as adjusted odds ratios (aORs).</div></div><div><h3>Results</h3><div>Each additional year of age was associated with substantially higher odds of being in a more advanced second-molar stage (aOR 5.41, 95 % CI 4.47–6.56). Males had lower odds than females of being in a higher stage (aOR 0.58, 95 % CI 0.41–0.81). Relative to children with no third-molar agenesis, the odds of being in a higher second-molar stage were progressively lower with greater agenesis: one missing (aOR 0.53, 95 % CI 0.31–0.91), two missing (aOR 0.39, 95 % CI 0.24–0.62), three missing (aOR 0.26, 95 % CI 0.11–0.62), and all four missing (aOR 0.04, 95 % CI 0.02–0.09).</div></div><div><h3>Conclusion</h3><div>Third-molar agenesis was associated with less advanced mandibular second-molar stages after accounting for age and sex. Agenesis status and extent may be relevant for the 14-year threshold. Future work should develop and externally validate prediction models that integrate third molar agenesis to improve accuracy and minimise misclassification.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106466"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145681571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the relationship between gut microbiota-metabolite axis and Jiawei Danxuan Koukang’s therapeutic effects in male rats with oral submucous fibrosis: A multi-omics analysis","authors":"Chenwei Wang ,&nbsp;Yuzhe Dai ,&nbsp;Yao Ye ,&nbsp;Qianqi Zeng ,&nbsp;Jin Tan","doi":"10.1016/j.archoralbio.2025.106480","DOIUrl":"10.1016/j.archoralbio.2025.106480","url":null,"abstract":"<div><h3>Objective</h3><div>This work aims to explore how Jiawei Danxuan Koukang (JDK) ameliorates Oral Submucous Fibrosis (OSF) via regulating the gut microbiota and its metabolites.</div></div><div><h3>Design</h3><div>We established an OSF rat model via oral mucosal arecoline injection. Rats were randomly divided into five groups: control, model, lycopene-treated, quercetin-treated, and JDK-treated. After intervention, we analyzed: serum metabolites by Liquid Chromatography-Mass Spectrometry -based untargeted metabolomics; gut microbiota profiling via 16S rDNA sequencing; oral mucosal histopathology and fibrosis using hematoxylin-eosin and Masson trichrome staining; expressions of fibrosis-related proteins (Transforming Growth Factor-β1 (TGF-β1), Collagen Type I Alpha 1 Chain (COL1A1)) by western blot; and cytokines (Interleukin-1β, Interleukin-6, Interleukin-10) in serum, oral and colon tissues via enzyme-linked immunosorbent assay.</div></div><div><h3>Results</h3><div>In the OSF model group, 120 serum metabolites were upregulated and 39 were down-regulated. When comparing the JDK group with the model group, 83 metabolites were upregulated and 132 were downregulated in the JDK group. Specifically, JDK targeted key metabolites: prostaglandins, leukotrienes, lysophosphatidylcholine, and 4-hydroxyproline. JDK also regulated two critical metabolic pathways: gly-cine-serine-threonine metabolism and tryptophan metabolism. JDK reduces the pro-inflammatory Ruminococcus and restores the butyrate-producing Lachnospiraceae_NK4A136_group. JDK downregulated Interleukin-1β and Interleukin-6 levels systemically and locally, concurrently increasing Interleukin-10, and reduced the expression of fibrosis-related proteins (TGF-β1, COL1A1) in the oral mucosa.</div></div><div><h3>Conclusions</h3><div>JDK alleviates OSF by normalizing inflammation and fibrosis-related metabolic pathways, linking gut microbiota remodeling to metabolic homeostasis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106480"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145770178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RAW 264.7-derived exosomal miR-494–3p regulates inflammation and osteogenic differentiation of human periodontal ligament stem cells through regulating CAMK2D","authors":"Yue Zhang,&nbsp;Yufu Liang,&nbsp;Yue Zhou","doi":"10.1016/j.archoralbio.2025.106457","DOIUrl":"10.1016/j.archoralbio.2025.106457","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to investigate the effect of M2-exos on osteogenic differentiation in human periodontal ligament stem cells (hPDLSCs) and the underlying mechanism.</div></div><div><h3>Design</h3><div>Exosomes were isolated from M2-polarized RAW 264.7 macrophages and characterized by transmission electron microscopy, nanoparticle tracking analysis, and Western blot. hPDLSCs were treated with M2-exos, and osteogenic differentiation was assessed using alkaline phosphatase and Alizarin Red S staining, along with reverse transcription-quantitative polymerase chain reaction analysis of osteogenic markers. Inflammation-related cytokines were measured by enzyme-linked immunosorbent assay. Bioinformatics analysis identified miR-494–3p as a key miRNA in M2-exos, and its interaction with calcium/calmodulin-dependent protein kinase II delta (CAMK2D) was validated by RNA pull-down and dual luciferase reporter assays. Functional experiments were performed using a miR-494–3p inhibitor and CAMK2D knockdown in hPDLSCs.</div></div><div><h3>Results</h3><div>M2-exos exhibited typical exosomal characteristics and were internalized by hPDLSCs. Additionally, M2-exos suppressed inflammation and enhanced osteogenic differentiation. Mechanistically, miR-494–3p was highly enriched in M2-exos and directly targeted CAMK2D. Moreover, inhibition of miR-494–3p exacerbated inflammation and impaired osteogenesis, while CAMK2D knockdown reversed these effects, restoring osteogenic potential.</div></div><div><h3>Conclusions</h3><div>RAW 264.7-derived exosomes inhibited inflammation and promoted osteogenic differentiation in hPDLSCs, a process that involves, at least in part, the delivery of miR-494–3p. These findings highlight a novel mechanism and the therapeutic potential of targeting the miR-494–3p/CAMK2D axis in periodontal bone regeneration.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106457"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145518981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral green and red propolis attenuate bone resorption and inflammation in experimental apical periodontitis","authors":"José Alex da Silva ,&nbsp;Letícia Cabrera Capalbo ,&nbsp;Renan Dal-Fabbro ,&nbsp;Romulo de Oliveira Sales-Junior ,&nbsp;Bharbara de Moura Pereira ,&nbsp;Edilson Ervolino ,&nbsp;João Eduardo Gomes-Filho ,&nbsp;Leopoldo Cosme-Silva","doi":"10.1016/j.archoralbio.2025.106479","DOIUrl":"10.1016/j.archoralbio.2025.106479","url":null,"abstract":"<div><h3>Objective</h3><div>To test whether systemic green or red propolis modulates inflammation and bone resorption in rat apical periodontitis (AP).</div></div><div><h3>Design</h3><div>Twenty-four male Wistar rats received AP induction in the first mandibular molars and were randomized to Control, Green propolis, or Red propolis (n = 8/group). Propolis (100 mg/kg in water) or vehicle was administered daily by gavage for 30 days. On day 30, periapical tissues were evaluated by micro-CT, histology (inflammatory score), and immunohistochemistry for RANKL, OPG, and TRAP-positive osteoclasts. Data were analyzed with Shapiro-Wilk, Kruskal-Wallis/Dunn, or one-way ANOVA/Tukey (α=0.05).</div></div><div><h3>Results</h3><div>Both propolis groups showed significantly less periapical bone resorption than the control group on micro-CT analysis (p &lt; 0.05). Histological evaluation revealed predominantly chronic inflammatory infiltrates, with significantly lower inflammation scores in the propolis-treated groups (p &lt; 0.05). Immunohistochemical analysis demonstrated a significant reduction in RANKL expression, an increase in OPG levels, and fewer TRAP-positive multinucleated cells in both propolis groups compared to the control (p &lt; 0.05). No significant differences were observed between green and red propolis.</div></div><div><h3>Conclusion</h3><div>Thirty days of systemic green or red propolis similarly attenuated periapical inflammation and osteoclast-mediated bone resorption in experimental AP, reflected by lower resorption volumes, reduced RANKL/TRAP, and higher OPG, indicating that propolis may serve as a host-modulatory adjunct to preserve periapical bone.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106479"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145733278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temporal dynamics and predictive modeling of oral epithelial dysplasia features during carcinogenesis","authors":"André Luis Santana de Freitas ,&nbsp;Gustavo Guth ,&nbsp;Anna Luíza Damaceno Araújo ,&nbsp;Marília De Marco Pinto Paiva dos Santos ,&nbsp;Giovana Radomille Tofoli ,&nbsp;Aguinaldo Silva Garcez ,&nbsp;Victor Angelo Martins Montalli ,&nbsp;Marcelo Sperandio","doi":"10.1016/j.archoralbio.2025.106444","DOIUrl":"10.1016/j.archoralbio.2025.106444","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the temporal evolution and predictive value of individual histopathological features of oral epithelial dysplasia (OED) during carcinogenesis, and to evaluate the diagnostic utility of optical fluorescence imaging in a murine model.</div></div><div><h3>Design</h3><div>A 4-nitroquinoline 1-oxide (4NQO) mouse model was used to induce oral squamous cell carcinoma (OSCC). Histological evaluation of 20 architectural and cytological OED features was performed at seven time points over 24 weeks. Data were analyzed using descriptive statistics, Spearman's correlation, logistic regression, and principal component analysis (PCA). A random forest model was developed to classify malignant transformation and evaluated using F1-score, cross-validation, ROC, and precision-recall curves. Optical fluorescence imaging was assessed for early lesion detection.</div></div><div><h3>Results</h3><div>Cumulative feature burden, particularly involving loss of stratification, reverse polarity, nuclear atypia, and mitotic activity, was more predictive of transformation than any single feature. PCA revealed two major axes—structural disorganization and proliferative instability. The random forest model achieved high predictive performance (F1 = 0.88, ROC-AUC = 0.97, PR-AUC = 0.98). Autofluorescence failed to improve early detection.</div></div><div><h3>Conclusion</h3><div>Feature accumulation is a robust predictor of OSCC risk in dysplastic lesions. Histological quantification combined with machine learning offers potential for improved prognostic modeling in oral precancer.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106444"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145500259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Escitalopram exposure compromises osteogenic potential of human osteoblastic cells","authors":"Augusto Del Pintor Pasotti ,&nbsp;Rodrigo Mendes Ferreiro Girondo ,&nbsp;Bruno Haddad ,&nbsp;Marcelo Franchin ,&nbsp;Bruna Benso ,&nbsp;Rogerio Heládio Lopes Motta ,&nbsp;Henrique Ballassini Abdalla","doi":"10.1016/j.archoralbio.2025.106481","DOIUrl":"10.1016/j.archoralbio.2025.106481","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to assess the impact of escitalopram on bone metabolism by evaluating its effects on cell viability and proliferation, wound-healing capacity, osteogenic activity, bone formation markers, and collagen deposition.</div></div><div><h3>Design</h3><div>The effects of escitalopram were studied on human osteoblastic SAOS-2 cells. Escitalopram (1–1000 µM) was tested in a dose–response curve. Cell viability was measured by MTT assay, and proliferation by hemocytometer counting. Cell migration was examined with the Scratch assay over 72 h. Osteogenic differentiation was assessed by gene expression of RUNX2, Osterix (Osx), bone sialoprotein (BSP), type I collagen (COL1), and osteocalcin (OCN) using RT-qPCR. Alkaline phosphatase (ALP) activity was analyzed at 4 and 8 days. Mineralization was determined by Alizarin Red staining (days 10, 14, 21). For last, Immunofluorescence was carried out for collagen 1 staining (days 3, 7 and 10).</div></div><div><h3>Results</h3><div>Escitalopram induced cytotoxicity in doses greater than 100 µM, reducing cell viability within 24 h. At non-toxic concentrations (≤30 µM), proliferation was enhanced in 30 µM after 7 days. Conversely, escilalopram reduced the migration capacity in a concentration-dependent manner. Moreover, the gene expression of RUNX2, OSX, BSP, COL1, and OCN were diminished when exposed to escitalopram. In the functional tests, escitalopram significantly decreases ALP activity at day 4, but not at day 8. Mineralization was dose-dependently impaired at 14 and 21 days. Collagen type I immunofluorescence exhibit weaker staining when escitalopram exposure.</div></div><div><h3>Conclusion</h3><div>Escitalopram compromises osteoblast differentiation, extracellular matrix formation, and migratory potential. These results provide mechanistic insight into the adverse skeletal effects of SSRIs and suggest the need for monitoring bone health in long-term users.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"182 ","pages":"Article 106481"},"PeriodicalIF":2.1,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145783949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}