Archives of oral biology最新文献

{"title":"Genetic evidence strengthens the connection between emotional disorders and TMD-related pain: A two-sample Mendelian randomization study","authors":"Kang Yu ,&nbsp;Huihuan Luo ,&nbsp;Jie Zhao ,&nbsp;Yiqun Wu ,&nbsp;Dedong Yu","doi":"10.1016/j.archoralbio.2024.106150","DOIUrl":"10.1016/j.archoralbio.2024.106150","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aims to evaluate the genetic association between emotional disorders and TMD-related pain through two-sample Mendelian randomization analysis.</div></div><div><h3>Design</h3><div>Single-nucleotide polymorphisms (SNPs) related to emotional disorders (worry, nerves, or depression) were selected from genome-wide association studies (GWAS) from UK Biobank consortia, and related these to SNPs from FinnGen consortia. The inverse-variance weighted (IVW) was used as the primary effect estimate between emotional disorders and TMD-related pain, and various methods were applied to test the reliability and stability of the results, namely MR-Egger and weighted median.</div></div><div><h3>Results</h3><div>The Mendelian randomization analysis showed that there was a positive correlation between emotional disorders and TMD-related pain, including worry group (IVW odds ratio (OR) = 3.86, 95 % confidence interval (CI) = 1.67–8.91), nerves group (IVW OR = 11.20, 95 % CI=2.04–61.64) and depression group (IVW OR = 3.32, 95 % CI=1.24–8.90). MR-Egger intercept and MR-PRESSO global test did not suggest evidence of horizontal or directional pleiotropy. Cochran’s Q test showed that there was no heterogeneity between instrumental variables.</div></div><div><h3>Conclusions</h3><div>This study provides genetic evidence that strengthens the connection between emotional disorders and TMD-related pain, which has important implications at the causal level as well as throughout the treatment process of TMD-related pain.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106150"},"PeriodicalIF":2.2,"publicationDate":"2024-12-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Paving the way for the use of Statherin-Derived Peptide (StN15) to control caries through acquired pellicle and biofilm microbiome engineering: Proof-of-concept in vitro/in vivo studies","authors":"Tamara Teodoro Araujo ,&nbsp;Ana Luiza Bogaz Debortolli ,&nbsp;Thamyris Souza Carvalho ,&nbsp;Chelsea Maria Vilas Boas Feitosa Rodrigues ,&nbsp;Aline Dionizio ,&nbsp;Beatriz Martines de Souza ,&nbsp;Mariele Vertuan ,&nbsp;Talita Mendes Ventura ,&nbsp;Larissa Tercilia Grizzo ,&nbsp;Reinaldo Marchetto ,&nbsp;Flavio Henrique Silva ,&nbsp;Marcos Chiaratti ,&nbsp;Angélica Camargo Santos ,&nbsp;Lindomar Oliveira Alves ,&nbsp;Milene Ferro ,&nbsp;Marília Afonso Rabelo Buzalaf","doi":"10.1016/j.archoralbio.2024.106159","DOIUrl":"10.1016/j.archoralbio.2024.106159","url":null,"abstract":"<div><h3>Objective</h3><div>This proof-of-concept sequence of <em>in vivo</em>/<em>in vitro</em> studies aimed to unveil the role of acquired enamel pellicle (AEP) engineering with statherin-derived peptide (StN15) on the AEP protein profile, enamel biofilm microbiome <em>in vivo</em> and on enamel demineralization in <em>vitro</em>.</div></div><div><h3>Design</h3><div><em>In vivo</em> studies, 10 volunteers, in 2 independent experiments (2 days each), rinsed (10 mL,1 min) with: deionized water (negative control) or 1.88 × 10⁻⁵ M StN15. The AEP, formed along 2 h and the biofilm, along 3 h, were collected. AEP was analyzed by quantitative shotgun-label-free proteomics. The enamel biofilm microbiome was evaluated using 16S-rRNA Next Generation Sequencing (NGS). An <em>in vitro</em> model with microcosm biofilm was employed. Bovine enamel samples (n = 72) were treated with 1) Phosphate-Buffer-Solution (PBS), 2) 0.12 %Chlorhexidine, 3) 500ppmNaF; 4) 1.88 × 10⁻⁵MStN15; 5) 3.76 × 10⁻⁵MStN15 and 6) 7.52 × 10⁻⁵MStN15. Biofilm was supplemented with human saliva and McBain saliva and cultivated for 5 days. Resazurin, colony forming units (CFU) and Transversal Microradiography Analysis-(TMR) were performed.</div></div><div><h3>Results</h3><div>Proteomic results showed several proteins with acid-resistant, calcium-binding, and antimicrobial properties in the StN15 group. The microbiome corroborated these findings, reducing bacteria that are closely related to dental caries in the StN15 group, compared to the PBS. The microcosm biofilm showed that the lowest concentration of StN15 was the most efficient in reducing bacterial activity, CFU and enamel demineralization compared to PBS.</div></div><div><h3>Conclusion</h3><div>StN15 can effectively alter the AEP proteome to inhibit initial bacterial colonization, thereby mitigating enamel demineralization. Future research should explore clinical applications and elucidate the mechanisms underlying the protective effects of StN15.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106159"},"PeriodicalIF":2.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness in root canal disinfection and biocompatibility of a final in vitro irrigation protocol based on cellulases and a hyperosmotic solution","authors":"Selene Velázquez-Moreno ,&nbsp;Norma V. Zavala-Alonso ,&nbsp;Ricardo Oliva Rodríguez ,&nbsp;Roberto Sánchez Sánchez ,&nbsp;Carlos Martín Torre Morales ,&nbsp;Omar Gonzalez-Ortega ,&nbsp;Fidel Martinez-Gutierrez","doi":"10.1016/j.archoralbio.2024.106157","DOIUrl":"10.1016/j.archoralbio.2024.106157","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the antimicrobial capacity and cell viability of a final irrigation protocol based on the use of a hydrolases enzymes mixture (HEM) and a hyperosmotic solution (HS) as an alternative to conventional protocols.</div></div><div><h3>Methods</h3><div>Root canals from 28 human first molars were used to develop multispecies anaerobic biofilms in standard reactors and irrigated with various protocols according to the following groups. Group A: control (sterile saline), group B: 2.25 % NaOCl, group C: 1 % NaOCl, group D: HS, group E: 100 U/mL HEM + 1 % NaOCl, group F: 100 U/mL HEM + HS, or group G: 100 U/mL HEM. The disinfection evaluation per group was carried out by CFU counting and Scanning Electron Microscopy (SEM). The viability was determined on fibroblasts.</div></div><div><h3>Results</h3><div>The F group, which consisted in irrigating with HEM + HS, had a biofilm elimination of over 5.33 (Log reduction), as well as the groups treated with NaOCl with eliminations of up to 5.34 (Log reduction). In addition, the evaluation of viability reflects a biocompatibility of the F group treatment, as opposed to the groups treated with NaOCl.</div></div><div><h3>Conclusions</h3><div>The irrigation protocols with HEM+HS and HEM+NaOCl turned out to be as efficient as the conventional protocol using NaOCl; moreover, the irrigation protocol with HEM+HS had low cell cytotoxicity in the viability assay when compared to cell cultures exposed to NaOCl.</div></div><div><h3>Clinical significance</h3><div>It is imperative that new and innovative ways are found for root canal therapy to ensure that the root canal system can be thoroughly cleaned.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106157"},"PeriodicalIF":2.2,"publicationDate":"2024-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142808834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profiling mRNA encoding glucocorticoid receptor α in saliva: Relationship to hair cortisol levels in individuals aged 15–25 years","authors":"Naima Abouseta ,&nbsp;Noha Gomaa ,&nbsp;Ali Tassi ,&nbsp;S. Jeffrey Dixon ,&nbsp;Krishna Singh ,&nbsp;Sharat C. Pani","doi":"10.1016/j.archoralbio.2024.106158","DOIUrl":"10.1016/j.archoralbio.2024.106158","url":null,"abstract":"<div><h3>Objective</h3><div>We assessed levels of mRNA encoding two glucocorticoid receptor (GR) isoforms (GRα and GRβ) in saliva and examined their relationship with hair cortisol levels and dental caries experience.</div></div><div><h3>Design</h3><div>Adolescents and young adults were assessed for dental caries experience, and hair cortisol was measured by ELISA. RNA was extracted from whole saliva using TRIzol, followed by quantitative real-time PCR analysis of GRα, GRβ, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH).</div></div><div><h3>Results</h3><div>GRβ mRNA was not detectable in most samples, whereas GRα mRNA was observed in all samples. There were significantly lower levels of GRα mRNA in individuals with elevated hair cortisol levels than in those with normal cortisol levels. Levels of GRα mRNA did not differ significantly in individuals with dental caries experience compared to individuals with no caries experience.</div></div><div><h3>Conclusions</h3><div>We identified and quantified mRNA encoding GRα in saliva. Its levels were inversely associated with hair cortisol (a marker of chronic stress). Although caries experience was associated with hair cortisol levels, there was no significant association between GRα levels and caries experience. Chronic stress has been proposed to be associated with reduced expression of GRα and this association appears to hold for GRα mRNA levels in saliva.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106158"},"PeriodicalIF":2.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of combining soluble calcium and pyrophosphate with sodium fluoride in inhibiting enamel caries progression: An in vitro study","authors":"Masashi Fujiki,&nbsp;Chika Akabane,&nbsp;Mitsuo Kimura,&nbsp;Kei Kurita","doi":"10.1016/j.archoralbio.2024.106156","DOIUrl":"10.1016/j.archoralbio.2024.106156","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to compare the effects of a combination of sodium fluoride, soluble calcium, and pyrophosphate (FCaP) versus fluoride alone in inhibiting enamel caries progression.</div></div><div><h3>Design</h3><div>Different FCaP solutions were prepared, and two were selected for testing (FCaP-1: F = 76 mmol/L, Ca = 7.6 mmol/L, P = 7.6 mmol/L, FCaP-5: F = 76 mmol/L, Ca = 23 mmol/L, P = 23 mmol/L). Fluoride solution (F = 76 mmol/L) was used as a control. Fluoride and calcium bioavailability in the solutions were measured, and NMR analysis was used to identify fluorine-containing complexes. Sound bovine enamel samples (n = 24 / group) underwent a 4-day pH cycling protocol followed by an additional 3 days of demineralization. Micro-hardness testing and fluoride concentration measurements were performed.</div></div><div><h3>Results</h3><div>FCaP-1 and FCaP-5 demonstrated nearly 100 % fluoride and calcium bioavailability. NMR analysis confirmed the formation of fluorine-containing complex. Enamel treated with FCaP-5 exhibited significantly less reduction in subsurface hardness after pH cycling and additional demineralization compared to fluoride alone. Interestingly, fluoride concentration and acid resistance on enamel surfaces treated with FCaP-5 was lower than with fluoride alone (Steel’s multiple comparison test, p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>FCaP effectively inhibits caries progression in subsurface enamel layers under pH cycling conditions by providing bioavailable calcium, indicating that FCaP increases the effectiveness of fluoride in caries management. FCaP may be a valuable addition to clinical practice, particularly for improving the effectiveness of fluoride-containing oral care products in individuals with low salivary calcium levels.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106156"},"PeriodicalIF":2.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study","authors":"Rafaela de Albuquerque Dias ,&nbsp;Karolyny Martins Balbinot ,&nbsp;Karine Duarte da Silva ,&nbsp;Ana Paula Neutzling Gomes ,&nbsp;Carla Mosconi ,&nbsp;Elismauro Franscisco de Mendonça ,&nbsp;Sandra Beatriz Chaves Tarquinio ,&nbsp;Sérgio de Melo Alves Junior ,&nbsp;Maria Cássia Ferreira de Aguiar ,&nbsp;João de Jesus Viana Pinheiro","doi":"10.1016/j.archoralbio.2024.106151","DOIUrl":"10.1016/j.archoralbio.2024.106151","url":null,"abstract":"<div><h3>Objective</h3><div>The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.</div></div><div><h3>Design</h3><div>Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.</div></div><div><h3>Results</h3><div>The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.</div></div><div><h3>Conclusions</h3><div>The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106151"},"PeriodicalIF":2.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The activation level of the floor-of-the-mouth muscles is systematically modulated using tongue-pressing and swallowing tasks in healthy elderly","authors":"Jong-Chi Oh","doi":"10.1016/j.archoralbio.2024.106155","DOIUrl":"10.1016/j.archoralbio.2024.106155","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to examine how muscle activity of the floor-of-the-mouth (FOM) muscles changes with different target exercise intensities of 40 %, 60 %, 80 %, and 100 % of maximum isometric pressure (MIP) during tongue-pressing and swallowing tasks in healthy elderly.</div></div><div><h3>Design</h3><div>This prospective, repeated-measures within-participant study included 35 participants (mean age: 75.2 ± 4.8 years, 26 women). Each participant performed 16 tasks using the Iowa Oral Performance Instrument (IOPI) in a randomized order, with two repetitions per task (anterior/posterior tongue × pressing/swallowing task × 40/60/80/100 % of the MIP). Furthermore, FOM-muscle activity during the task was simultaneously measured using surface electromyography. Statistical analysis was conducted using repeated-measures analysis of variance (ANOVA).</div></div><div><h3>Results</h3><div>Significant differences in FOM-muscle activity were observed across most intensity levels during pressing tasks (<em>p</em> &lt; 0.001), with fewer significant differences noted during swallowing tasks, particularly involving the posterior tongue. A significant correlation was found between tongue pressure of the anterior/posterior tongue and FOM-muscle activation level (<em>p</em> &lt; 0.001 and <em>r</em> = 0.517 and 0.447 fo<em>r</em> the isometric tongue pressure of the anterior and posterior tongue, respectively; <em>r</em> = 0.370 for the swallowing pressure of the anterior tongue) except for posterior tongue swallowing task.</div></div><div><h3>Conclusions</h3><div>These findings suggest that healthy elderly can systematically modulate suprahyoid muscle activity using a tongue-pressure-measurement device when performing accuracy training. However, they may require personalized systematic feedback and sufficient practice. The study underscores the potential of the IOPI in swallowing rehabilitation for elderly, while also highlighting the need for further research on patients with dysphagia.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106155"},"PeriodicalIF":2.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tensile stress induced osteogenesis of periodontal ligament cells via Piezo1 mediated TAZ-Cbfα1 signaling","authors":"Sisi Li ,&nbsp;Ting Jin ,&nbsp;Yi Wang ,&nbsp;Hui Deng ,&nbsp;Rongdang Hu","doi":"10.1016/j.archoralbio.2024.106152","DOIUrl":"10.1016/j.archoralbio.2024.106152","url":null,"abstract":"<div><h3>Objective</h3><div>Cyclic tensile stress (CTS) is known to induce osteogenesis of periodontal ligament cells (PDLCs), in which the molecular mechanism remains to be elucidated. This study aimed to investigate the role of the mechanosensitive calcium channel Piezo1 in the osteogenesis of PDLCs under tensile strain, as well as the signal regulation of the TAZ-Cbfα1 pathway in this process.</div></div><div><h3>Design</h3><div>PDLCs were isolated from periodontal ligament tissues and subjected to CTS. Alizarin red staining (ARS) and alkaline phosphatase (ALP) assay were used to detect the osteogenesis of PDLCs. RT-qPCR and Western blot were used to detect the transcripts and protein expression levels of Piezo1, Transcriptional co-activator with PDZ binding motif (TAZ), and Core-binding factor α1 (Cbfα1) respectively. Immunofluorescence staining was used to detect the nuclear aggregation of TAZ. Small interfering RNA (siRNA) targeting Piezo1 (Piezo1-siRNA) was adopted to inhibit Piezo1 mRNA expression.</div></div><div><h3>Results</h3><div>The results showed that the osteogenic differentiation capacity of PDLCs was significantly enhanced under CTS, along with elevated mRNA and protein expression levels of Piezo1, TAZ, and Cbfα1. Moreover, the ALP activity and the formation of calcium nodules by ARS staining were significantly increased. In addition, Piezo1 siRNA infection significantly inhibited the CTS-induced TAZ-Cbfα1 pathway and the osteogenesis of PDLCs, suggesting the regulatory role of Piezo1.</div></div><div><h3>Conclusions</h3><div>We provided evidence that the application of CTS encourages the osteogenic differentiation of PDLCs, which could be mediated by the Piezo1 targeted TAZ-Cbfα1 signaling.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106152"},"PeriodicalIF":2.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CD10-positive cells on osteogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells","authors":"Tomoaki Sakurai,&nbsp;Masakazu Ishii,&nbsp;Haruka Miyata,&nbsp;Nao Ikeda,&nbsp;Fumio Suehiro,&nbsp;Naohiro Komabashiri,&nbsp;Yurika Oura,&nbsp;Masahiro Nishimura","doi":"10.1016/j.archoralbio.2024.106135","DOIUrl":"10.1016/j.archoralbio.2024.106135","url":null,"abstract":"<div><h3>Objective</h3><div>This study was aimed at investigating the effect of CD10-positive cells within the maxillary/mandibular bone marrow-derived mesenchymal stem cells (MBMSCs) on osteogenic differentiation of MBMSCs.</div></div><div><h3>Design</h3><div>CD10 expression in iliac bone marrow-derived MSCs (IBMSCs), MBMSCs, and gingival fibroblasts was measured using flow cytometry. The osteogenic potential of 19 MBMSC lines was evaluated, and based on it, they were classified into osteogenic-High and osteogenic-Low groups. The percentage of CD10-positive cells in each group was compared. Effect of coculturing gingival fibroblasts and CD10-positive cells on the osteogenic potential of MBMSCs was also assessed. Expression of tissue inhibitor of metalloprotease-1 (TIMP-1) in osteogenic-High and osteogenic-Low MBMSCs was measured using quantitative real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. The molecular mechanisms underlying the regulation of osteogenic differentiation in MBMSCs were investigated.</div></div><div><h3>Results</h3><div>CD10 was not expressed in IBMSCs, but was highly expressed in fibroblasts. In MBMSCs, the CD10-positivity rate varied considerably between cells. MBMSCs with a high-CD10 positivity rate showed low osteogenic potential. Coculture with fibroblasts or CD10-positive cells reduced the osteogenic potential of MBMSCs. TIMP-1 was highly expressed in CD10-positive cells, and osteogenic-Low MBMSCs showed significantly higher TIMP-1 expression compared with osteogenic-High MBMSCs. β-catenin signaling was suppressed in osteogenic-Low MBMSCs.</div></div><div><h3>Conclusion</h3><div>This study revealed that TIMP-1 secreted from CD10-positive cells may be involved in the suppression of the osteogenic potential of MBMSCs by contamination with CD10-positive cells. This finding provides important insights for developing bone regeneration therapies using MBMSCs.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106135"},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary alpha amylase as a diagnostic biomarker for dental caries – A systematic review and meta analysis","authors":"S. Savitha Priyadarsini ,&nbsp;P.G. Naveen Kumar ,&nbsp;Mahesh R. Khairnar ,&nbsp;Zainab Akram ,&nbsp;Ridhi Ghodela ,&nbsp;Sachin Kumar Jadhav ,&nbsp;Neha Shukla","doi":"10.1016/j.archoralbio.2024.106136","DOIUrl":"10.1016/j.archoralbio.2024.106136","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review and meta-analysis aimed to assess the association between the dental caries and the level of salivary alpha amylase activity among caries active and caries free individuals.</div></div><div><h3>Design</h3><div>This review followed the PRISMA guidelines and registered in PROSPERO (CRD42024518973). A comprehensive search across various research databases until January 31, 2024 yielded 1031 articles, where multiple screening ultimately narrowed it to 13 articles. Appropriate cross sectional studies comparing caries active (CA), caries free (CF) group and evaluating their level of salivary alpha amylase activity were included.</div></div><div><h3>Results</h3><div>The meta-analysis was performed on all 13 included studies utilizing a random effects model and two sub group analysis were performed where studies with salivary alpha amylase activity estimated using spectrophotometer and autoanalyzer were analyzed separately among caries active and caries free group. The pooled data reveals that the salivary alpha amylase activity was significantly higher (p = 0.005) in caries active group compared to caries free group with a standardized mean difference of 1.30.</div></div><div><h3>Conclusion</h3><div>This review indicated the differences in the salivary alpha amylase activity among caries active and caries free individuals which could aid in identifying individuals susceptible to dental caries thereby offering new insights for preventive dentistry by assessing their caries risk, enabling tailored preventive strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106136"},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}