Archives of oral biology最新文献

{"title":"Influence of bone morphogenetic protein (BMP) signaling and masticatory load on morphological alterations of the mouse mandible during postnatal development","authors":"Amber Uptegrove ,&nbsp;Coral Chen ,&nbsp;Madison Sahagun-Bisson ,&nbsp;Anshul K. Kulkarni ,&nbsp;Ke’ale W. Louie ,&nbsp;Hiroki Ueharu ,&nbsp;Yuji Mishina ,&nbsp;Maiko Omi-Sugihara","doi":"10.1016/j.archoralbio.2024.106096","DOIUrl":"10.1016/j.archoralbio.2024.106096","url":null,"abstract":"<div><h3>Objective</h3><div>Bone homeostasis relies on several contributing factors, encompassing growth factors and mechanical stimuli. While bone morphogenetic protein (BMP) signaling is acknowledged for its essential role in skeletal development, its specific impact on mandibular morphogenesis remains unexplored. Here, we investigated the involvement of BMP signaling and mechanical loading through mastication in postnatal mandibular morphogenesis.</div></div><div><h3>Design</h3><div>We employed conditional deletion of <em>Bmpr1a</em> in osteoblasts and chondrocytes via <em>Osterix</em>-Cre. Cre activity was induced at birth for the 3-week group and at three weeks for the 9-week and 12-week groups, respectively. The conditional knockout (cKO) and control mice were given either a regular diet (hard diet, HD) or a powdered diet (soft diet, SD) from 3 weeks until sample collection, followed by micro-CT and histological analysis.</div></div><div><h3>Results</h3><div>The cKO mice exhibited shorter anterior lengths and a posteriorly inclined ramus across all age groups compared to the control mice. The cKO mice displayed an enlarged hypertrophic cartilage area along with fewer osteoclast numbers in the subchondral bone of the condyle compared to the control group at three weeks, followed by a reduction in the cartilage area in the posterior region at twelve weeks. Superimposed imaging and histomorphometrical analysis of the condyle revealed that BMP signaling primarily affects the posterior part of the condyle, while mastication affects the anterior part.</div></div><div><h3>Conclusions</h3><div>Using 3D landmark-based geometric morphometrics and histological assessments of the mandible, we demonstrated that BMP signaling and mechanical loading reciprocally contribute to the morphological alterations of the mandible and condyle during postnatal development.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106096"},"PeriodicalIF":2.2,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Roles of the histone methyltransferase SET domain bifurcated 1 in epithelial cells during tooth development” [Archives of Oral Biology (September 2024) 106026]","authors":"Yuri Takagiwa,&nbsp;Norihisa Higashihori,&nbsp;Sakurako Kano,&nbsp;Keiji Moriyama","doi":"10.1016/j.archoralbio.2024.106093","DOIUrl":"10.1016/j.archoralbio.2024.106093","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106093"},"PeriodicalIF":2.2,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142697864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Forensic significance of postmortem pink teeth: A narrative review","authors":"Susana Braga ,&nbsp;Inês Morais Caldas ,&nbsp;Ricardo Jorge Dinis-Oliveira","doi":"10.1016/j.archoralbio.2024.106092","DOIUrl":"10.1016/j.archoralbio.2024.106092","url":null,"abstract":"<div><h3>Objective</h3><div>To review the phenomenon of postmortem pink teeth, exploring its etiology, correlation with the cause of death, and the potential forensic significance of this medico-legal finding.</div></div><div><h3>Methods</h3><div>A comprehensive literature search was conducted using databases such as PubMed, Scopus, and B-on, employing keywords like \"Forensic Pathology,\" \"Forensic Dentistry,\" \"Pink Teeth,\" and \"Medico-legal aspects.\" The search included studies without temporal limits to gather extensive data on the postmortem pink teeth phenomenon.</div></div><div><h3>Results</h3><div>The postmortem pink teeth phenomenon is characterized by a red-pink discoloration of the dentin, typically sparing the enamel. It is most often observed in moist environments and cases involving water immersion, strangulation, and carbon monoxide poisoning. The study found no consistent relationship between the pink discoloration and specific causes of death, suggesting the phenomenon is more closely related to environmental conditions and the state of decomposition. The pink coloration is more prevalent in anterior, single-rooted teeth and younger individuals.</div></div><div><h3>Conclusion</h3><div>The postmortem pink teeth phenomenon remains a complex and enigmatic finding in forensic science. While it does not conclusively indicate a specific cause of death, understanding its occurrence can aid forensic investigations. Further research is needed to elucidate this phenomenon's mechanisms and validate its forensic relevance.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106092"},"PeriodicalIF":2.2,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924002139/pdfft?md5=84d57625ed9502825b3d929433edebc2&pid=1-s2.0-S0003996924002139-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142312449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dysregulation of a novel m6A regulator YWHAG is correlated with metastasis and poor prognosis in oral squamous cell carcinoma – A cross-sectional study","authors":"Rithanyaa Ramesh Kumar ,&nbsp;Balachander Kannan ,&nbsp;Chandra Pandi ,&nbsp;Anitha Pandi ,&nbsp;Vijayashree Priyadharsini Jayaseelan ,&nbsp;Paramasivam Arumugam","doi":"10.1016/j.archoralbio.2024.106090","DOIUrl":"10.1016/j.archoralbio.2024.106090","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to investigate the role of a novel m6A and cell cycle regulator YWHAG in oral squamous cell carcinoma (OSCC) by analyzing its expression and functional implications.</p></div><div><h3>Design</h3><p>Tumor samples (n = 51) and adjacent non-tumor samples (n = 38) were collected from patients with OSCC, and cell lines were processed. <em>YWHAG</em> mRNA expression was assessed using quantitative reverse transcription PCR (RT-qPCR) analysis. Various tools, such as UALCAN, Protein-Atlas analysis, TIMER 2.0, and other <em>in silico</em> tools, were used to explore clinicopathological correlations, protein expression, immune cell infiltration, and functional associations of YWHAG.</p></div><div><h3>Results</h3><p>YWHAG mRNA and protein expression were significantly upregulated in OSCC tumor tissues and OSCC cell lines compared to non-tumor tissues and normal cells (<em>p</em> &lt; 0.001). High <em>YWHAG</em> expression significantly correlated with advanced tumor stage, higher grade, lymph node metastasis, and poor prognosis (<em>p</em> &lt; 0.05). Functional analysis revealed that YWHAG is associated with pathways involved in aggressive cancer progression. YWHAG expression positively correlated with its target gene <em>CTTN</em> expression, which was also upregulated in OSCC and associated with poor prognosis (<em>p</em> &lt; 0.05).</p></div><div><h3>Conclusions</h3><p>Study findings indicate that YWHAG may contribute to the progression of OSCC and could be a potential therapeutic target or prognostic biomarker. Further investigation is necessary to understand the underlying mechanisms and assess the clinical implications of YWHAG dysregulation in OSCC.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106090"},"PeriodicalIF":2.2,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142241714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of cathepsin K-induced proteoglycans degradation on dentin collagen","authors":"Neng-wu Jiang ,&nbsp;Yi-ying Chen ,&nbsp;Xiu-jiao Lin ,&nbsp;Hao Yu","doi":"10.1016/j.archoralbio.2024.106091","DOIUrl":"10.1016/j.archoralbio.2024.106091","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to investigate the effects of cathepsin K (catK) on proteoglycans (PGs) and the subsequent impacts on dentin collagen degradation.</p></div><div><h3>Materials and Methods</h3><p>Demineralized dentin samples were prepared and divided into the following groups: deionized water (DW), 0.1 U/mL chondroitinase ABC (C-ABC), and 1 μM odanacatib (ODN). Then, they were immersed for 48 h and then incubated in 1 mL of PBS (pH=5.5) at 37 °C for 5 d. Glycosaminoglycan (GAG) were examined to explore the degradation of PGs by catK. To determine the effect of catK-mediated PGs on dentin collagen degradation, hydroxyproline (HYP) assays, assessment of the degree of dentin crosslinking, and scanning electron microscopy (SEM) were assessed. Statistical analysis was conducted using one-way ANOVA followed by Tukey’s tests or Welch's ANOVA followed by Dunnett's tests at a significance level of 0.05.</p></div><div><h3>Results</h3><p>The production of GAG was significantly lower in the ODN group than in the DW group (<em>P</em> &lt; 0.05), revealing that PG degradation was reduced in dentin after ODN treatment. Additionally, ODN treatment minimized the gaps in collagen fibers, improved fiber arrangement, and significantly increased the degree of collagen crosslinking, subsequently reducing the total amount of collagen fiber degradation in the dentin (<em>P</em> &lt; 0.05).</p></div><div><h3>Conclusions</h3><p>CatK-mediated degradation of PGs negatively impacted the stability of collagen fibers, promoted gaps, led to a less organized arrangement of dentin collagen fibers, ultimately increasing collagen degradation.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106091"},"PeriodicalIF":2.2,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142172946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical, pathohistological, radiographic and cardiological analysis reveals the possible association between apical periodontitis and cardiac function in diabetic rats","authors":"Khasan Alievich Ordashev ,&nbsp;Andjela Milojevic Samanovic ,&nbsp;Israpil Alisultanovich Omarov ,&nbsp;Vladimir Zivkovic ,&nbsp;Tamara Nikolic Turnic ,&nbsp;Jovana Novakovic ,&nbsp;Aleksandra Stojanovic ,&nbsp;Marina Nikolic ,&nbsp;Bozidar Pindovic ,&nbsp;Jasmina Sretenovic ,&nbsp;Vladimir Petrovich Fisenko ,&nbsp;Sergey Bolevich ,&nbsp;Stefani Bolevich ,&nbsp;Vladimir Jakovljevic","doi":"10.1016/j.archoralbio.2024.106089","DOIUrl":"10.1016/j.archoralbio.2024.106089","url":null,"abstract":"<div><h3>Objective</h3><p>To evaluate the possible effects of apical periodontitis (AP) on cardiac function, structure, and oxidative stress (OS) in rats with diabetes mellitus type 2 (T2DM).</p></div><div><h3>Design</h3><p>Forty-eight (<em>Wistar albino</em>, male) rats were randomized into four groups: control healthy (CTRL), normoglycemic with AP (AP), T2DM, and T2DM with AP (T2DM+AP). T2DM was induced by streptozotocin and a high-fat diet. AP was induced by pulp exposure to the oral environment for 4 weeks and analyzed radiographically. In the blood samples insulin and glucose were established. <em>In vivo,</em> cardiac function was evaluated by echocardiography. <em>Ex vivo</em> cardiac function was assessed by the Langendorff technique. Heart tissue was analyzed pathophysiologically. OS was determined in cardiac tissue homogenate and coronary venous effluent, spectrophotometrically.</p></div><div><h3>Results</h3><p>Impaired glycoregulation was observed in the T2DM+AP group compared to the T2DM, AP, and CTRL groups. The T2DM+AP group was associated with disturbed echocardiography and cardiodynamic parameters. The levels of superoxide anion radical, nitrite, and index of lipid peroxidation were significantly increased, while the superoxide dismutase and catalase were significantly decreased in the T2DM+AP group compared to T2DM, AP, and CTRL groups. The radiographic AP area was significantly larger in the T2DM+AP compared to the AP group.</p></div><div><h3>Conclusion</h3><p>AP was associated with increased glucose levels, impaired cardiac function, structure, and OS in diabetic rats. Diabetes was related to an increased radiographic AP area. The study may be a starting point for further research to clarify the effects of AP on cardiac function in various models of systemic diseases.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106089"},"PeriodicalIF":2.2,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enamel caries lesions, with and without initial cavitation, in relation to anatomical areas of the proximal surface in deciduous molars","authors":"Basílio Rodrigues Vieira ,&nbsp;Leonardo de Freitas Ferreira ,&nbsp;Nathalie Murielly Rolim de Abreu ,&nbsp;Frederico Barbosa de Sousa","doi":"10.1016/j.archoralbio.2024.106081","DOIUrl":"10.1016/j.archoralbio.2024.106081","url":null,"abstract":"<div><h3>Objective</h3><p>proximal enamel caries lesions (PEC) are believed to initiate and progress to cavitation below the proximal contact area (PCA), but no evidence exists on the location of initial carious cavitation on the proximal surface with functional PCA. This study aimed to test the association of anatomical areas of the proximal surface with the severity of PEC and the frequency of cavitation in PEC in primary molars</p></div><div><h3>Design</h3><p>laboratory, observational, transversal study. Exfoliated primary molars (n = 33) with functional PCA (biofilm-free PCA surrounded by biofilm) had their proximal surfaces (one/tooth) divided anatomically into up to nine areas: 3 areas based on the occlusal/cervical PCA boundaries (areas I, II, and III; occluso-cervically) and 3 areas based on the bucco/lingual PCA boundaries (A, B, and C), with area IIB representing the PCA and area IIIB as the sub-PCA (below the PCA). PEC (ICDAS scores 1 and 2–3) and cavitation in PEC were quantified in all areas using stereomicroscopy and microCT. PEC volume was quantified in areas IIB and IIIB under microCT</p></div><div><h3>Results</h3><p>PEC severity increased occluso-cervically. PCA and sub-PCA presented different PEC severities (higher in sub-PCA) and similar PCE volumes, but the odds of carious cavitation were much higher (Odds ratio = 197.4; 95 % CI: 8.7/4480.7) in the PCA than in the sub-PCA (no cavitation).</p></div><div><h3>Conclusion</h3><p>PCA presented lower PEC severity and similar PEC volume compared to sub-PCA, but PCA concentrated all cavitations in PEC, supporting a new model for the pathogenesis of PEC.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106081"},"PeriodicalIF":2.2,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal expression of Sox2+ progenitor cells regulates the regeneration of rat submandibular gland","authors":"Dan Li ,&nbsp;Yi Qu ,&nbsp;Bin Wang ,&nbsp;Haoyang Zhang ,&nbsp;Lizheng Qin","doi":"10.1016/j.archoralbio.2024.106080","DOIUrl":"10.1016/j.archoralbio.2024.106080","url":null,"abstract":"<div><h3>Objective</h3><p>Sox2 plays crucial roles in tissues homeostasis and regeneration. However, there are lack of a comprehensive examination of Sox2 expression and its functional role in submandibular gland regeneration. Therefore, we aimed to elucidate the impact of Sox2 on submandibular gland regeneration.</p></div><div><h3>Materials and Methods</h3><p>A Sprague-Dawley rat submandibular gland duct ligation/de-ligation regeneration model was conducted in this study. Sox2-shRNA vectors were retro-ductally administered into the submandibular gland to establish a stable Sox2 knockdown model. Conventional histopathological and molecular biological methods were used to investigate phenotypic changes.</p></div><div><h3>Results</h3><p>The submandibular gland normalized completely 28 days after ligature removal (following 7 days of duct ligation). AQP5 expression gradually increased after ligation removal until returning to normal levels. In submandibular gland regeneration, Sox2 re-expressed and co-expressed with AQP5⁺ acinar cells, and Sox2 expression peaked on day 14, recovered to normal on day 28, reproducing the developmental pattern. Sox2 knockdown hindered gland regeneration and induced irreversible fibrosis. The AQP5 expression was significantly lower than the contemporaneous solely ligated group, while the blue collagen deposition and the Vimentin expression increased prominently. The expression of CD68, IL-1β, TNF-α and IL-17A increased significantly, and epithelial cells in the Sox2 knockdown group expressed higher levels of IL-17A.</p></div><div><h3>Conclusions</h3><p>These findings highlight Sox2 as a crucial regulator of the acinar cell lineage. Sox2⁺ progenitor cells are pivotal for acinar cell maintenance, which is indispensable for submandibular gland regeneration. Collectively, our findings may help develop targeted interventions for enhancing tissue repair and preventing irreversible fibrosis in salivary gland disorders.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106080"},"PeriodicalIF":2.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142096646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiota: Taxonomic composition and functional profile in caries-free and in caries-affected individuals – A systematic review","authors":"Maria Eduarda Lisbôa Pagnussatti ,&nbsp;Heitor Sales de Barros Santos ,&nbsp;Clarissa Cavalcanti Fatturi Parolo ,&nbsp;Juliana Balbinot Hilgert ,&nbsp;Rodrigo Alex Arthur","doi":"10.1016/j.archoralbio.2024.106070","DOIUrl":"10.1016/j.archoralbio.2024.106070","url":null,"abstract":"<div><h3>Objective</h3><p>To compare the oral microbiota among caries-free (CF) with caries-affected (CA) individuals, both at taxonomic and at functional levels.</p></div><div><h3>Design</h3><p>This systematic review was conducted following PRISMA guidelines. A structured search was carried out in MEDLINE/PUBMED, Web of Science, EMBASE, LILACS, SciELO, Scopus and Google Scholar databases up to September, 2023. Observational studies, without any restriction on date of publication and using next-generation targeted or untargeted sequencing methods for identification of microbial communities were included. Qualitative synthesis was performed from all included studies.</p></div><div><h3>Results</h3><p>54 studies were included (43 cross-sectional; 11 cohort) comprising more than 3486 participants (at least 1666 CF and 1820 CA) whose saliva and/or dental plaque were used as clinical samples. Methodological quality was graded as “fair” for most of the studies. The abundance of 87 bacterial and 44 fungal genera were statistically different among CF and CA individuals. <em>Atopobium</em> spp., <em>Capnocytophaga</em> spp., <em>Lactobacillus</em> spp., <em>Prevotella</em> spp., <em>Scardovia</em> spp., <em>Selenomonas</em> spp. among others were frequently reported as being more abundant in CA individuals. Several functional patterns, such as lipids, carbohydrate, starch, sucrose, amino sugar metabolisms, among others, were identified as being specifically related to CF or to CA conditions.</p></div><div><h3>Conclusion</h3><p>In spite of the variability among the included studies and of the predominance of qualitative synthesis, groups of microorganisms as well as specific functional profiles coded by the assessed microbiota are differently abundant among caries-affected and caries-free individuals. These results need to be interpreted with caution considering the limitations inherent to each assessed primary study.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106070"},"PeriodicalIF":2.2,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCKR signaling map, G-Protein bindings, hormonal regulation, and neural mechanisms may influence the osteogenic/cementogenic differentiation potential of hPDLSCs","authors":"Catharina Marques Sacramento ,&nbsp;Miki Taketomi Saito ,&nbsp;Márcio Zaffalon Casati ,&nbsp;Enilson Antonio Sallum ,&nbsp;Renato Correa Viana Casarin ,&nbsp;Karina Gonzales Silvério","doi":"10.1016/j.archoralbio.2024.106069","DOIUrl":"10.1016/j.archoralbio.2024.106069","url":null,"abstract":"<div><h3>Objective</h3><p>Periodontal regeneration poses challenges due to the periodontium's complexity, relying on mesenchymal cells from the periodontal ligament (hPDLSCs) to regenerate hard tissues like bone and cementum. While some hPDLSCs have high regeneration potential (HOP-hPDLSCs), most are low potential (LOP-hPDLSCs). This study analyzed hPDLSCs from a single donor to minimize inter-individual variability and focus on key differences in differentiation potentials.</p></div><div><h3>Design</h3><p>This study used RNA-seq, genomic databases, and bioinformatics tools to explore signaling pathways (SPs), biological processes (BPs), and molecular functions (MFs) guiding HOP cells to mineralized matrix production. It also investigated limitations of LOP cells and strategies for enhancing their osteo/cementogenesis.</p></div><div><h3>Results</h3><p>In basal conditions, HOP exhibited a multifunctional gene network with higher expression of genes related to osteo/cementogenesis, cell differentiation, immune modulation, stress response, and hormonal regulation. In contrast, LOP focused on steroid hormone biosynthesis and nucleic acid maintenance. During osteo/cementogenic induction, HOP showed strong modulation of genes related to angiogenesis, cell division, mesenchymal differentiation, and extracellular matrix production. LOP demonstrated neural synaptic-related processes and preserved cellular cytoskeleton integrity. CCKR map signaling and G-protein receptor bindings gained significance during osteo/cementogenesis in HOP-hPDLSCs. Both HOP and LOP shared common BPs related to gastrointestinal and reproductive system development.</p></div><div><h3>Conclusion</h3><p>The osteo/cementogenic differentiation of HOP cells may be regulated by CCKR signaling, G-protein bindings, and specific hormonal regulation. LOP cells seem committed to neural mechanisms. This study sheds light on hPDLSCs' complex characteristics, offering a deeper understanding of their differentiation potential for future periodontal regeneration research and therapies.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106069"},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}