Archives of oral biology最新文献

{"title":"Bioinformatics-driven salivary microbial and functional profiling for identifying biomarkers in oral cancer and tobacco abusers in the Indian population","authors":"Chakresh Kumar Jain ,&nbsp;Sarita Maurya ,&nbsp;Ankita Singh","doi":"10.1016/j.archoralbio.2025.106346","DOIUrl":"10.1016/j.archoralbio.2025.106346","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate alterations in the salivary microbiome and its functional potential in Oral Squamous Cell Carcinoma (OSCC) patients, tobacco abusers, and healthy individuals in order to identify microbial biomarkers and gain insight into OSCC pathogenesis.</div></div><div><h3>Design</h3><div>Saliva-derived 16S rRNA gene sequences from OSCC patients, tobacco users, and healthy controls were obtained from the Indian Biological Data Centre (IBDC). Taxonomic classification was performed using QIIME2, and functional prediction was conducted with MicFunPred. Alpha and beta diversity, differential abundance, and pathway enrichment analyses were used to compare microbial communities and functions among the groups.</div></div><div><h3>Results</h3><div>OSCC samples showed significantly reduced alpha diversity and distinct microbial community profiles relative to healthy controls. Genera including <em>Porphyromonas</em>, <em>Streptococcus</em>, <em>Rothia</em>, and <em>Parvimonas</em> were enriched in OSCC and are associated with inflammation and carcinogenesis. Tobacco users exhibited increased microbial richness, with dominance of <em>Prevotella</em>, <em>Veillonella</em>, and <em>Peptostreptococcus</em>, indicative of adaptation to chemical exposure. Functional predictions in OSCC samples indicated enrichment in pathways related to bacterial chemotaxis, lipopolysaccharide biosynthesis, glycolysis, and DNA repair. Tobacco-associated microbiota showed elevated oxidative stress response and detoxification pathways.</div></div><div><h3>Conclusions</h3><div>Microbial dysbiosis and functional alterations in the oral microbiome are strongly associated with OSCC and tobacco use. The identification of disease-specific microbial signatures and pathways highlights the potential of the oral microbiome as a non-invasive biomarker and therapeutic target for precision medicine in OSCC, particularly in the Indian population.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106346"},"PeriodicalIF":2.2,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COL1-related overlap disorder: An emerging phenotype linked to mono- and bi-allelic COL1A1/2 variants","authors":"Nehal F. Hassib ,&nbsp;Rasha M. Elhossini ,&nbsp;Inas S.M. Sayed ,&nbsp;Mona S. Aglan ,&nbsp;Mohamed S. Abdel-Hamid","doi":"10.1016/j.archoralbio.2025.106344","DOIUrl":"10.1016/j.archoralbio.2025.106344","url":null,"abstract":"<div><h3>Objectives</h3><div>We describe the clinical, radiological, and oro-dental findings of four unrelated families with unusual skeletal phenotypes caused by mono- and bi- allelic <em>COL1A1/2</em> variants.</div></div><div><h3>Design</h3><div>The study included five Arab patients with clinical manifestations resembling osteogenesis imperfecta in the presence of variable degrees of osteoporosis, bone deformities, gray sclera, Wormian bones in the skull, and oro-dental abnormalities. However, bone fractures, which are considered the cardinal feature in osteogenesis imperfecta patients, were not documented in any of the cases. In addition, they also lacked the characteristic features of Ehlers-Danlos syndrome. Exome sequence was used to identify the causative variants.</div></div><div><h3>Results</h3><div>A new homozygous missense variant in <em>COL1A1</em>: c.4340 T &gt; G; p.(Val1447Gly) was identified in one family, while the remaining three families harbored two recurrent and one novel heterozygous variants in <em>COL1A2</em>: [c.1171 G&gt;A; p.(Gly391Ser) and c.1253 G&gt;C; p.(Gly418Ala)] and <em>COL1A1</em>: c.1678 G&gt;A; p.(Gly560Ser), respectively.</div></div><div><h3>Conclusions</h3><div>We present a new patient harboring a homozygous <em>COL1A1</em> variant with a distinctive skeletal phenotype in comparison to the few previously reported patients in the literature. In addition, we describe three families with osteogenesis imperfecta like phenotype harboring monoallelic <em>COL1A1/2</em> variants, expanding the spectrum of COL1-related overlap disorder.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106344"},"PeriodicalIF":2.2,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144523116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory, antioxidant, and antimicrobial evaluation of morin","authors":"Luciana Solera Sales ,&nbsp;Benjamin Hewitt ,&nbsp;Maria Muchova ,&nbsp;Fernanda Lourenção Brighenti ,&nbsp;Sarah A. Kuehne ,&nbsp;Melissa M. Grant ,&nbsp;Michael R. Milward","doi":"10.1016/j.archoralbio.2025.106343","DOIUrl":"10.1016/j.archoralbio.2025.106343","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to evaluate the anti-inflammatory and antioxidant activity of morin in an epithelial cell culture inflammatory model, as well as its antimicrobial activity against periodontal pathogens. Effects of morin alone or slow-release from a polymer-based formulation were compared.</div></div><div><h3>Methods</h3><div>An oral epithelial cell line (H400) was pre-conditioned with 0.125 mg/mL morin (free morin or morin in the formulation) for 8 h. The cells were challenged with heat-killed <em>Fusobacterium nucleatum</em> and <em>Porphyromonas gingivalis</em> (MOI 100:1) for 24 h. The production of GM-CSF was measured by enzyme-linked immunosorbent assay (ELISA) and the gene expression levels of IL-8, GM-CSF, IL1B, NF-kB and NLRP3 were evaluated by RT – PCR. Neutrophils were pre-conditioned with 0.01 mg/mL morin and stimulated immediately with heat-killed <em>F. nucleatum</em> or <em>P. gingivalis</em> (MOI 100:1) for 2.5 h and then total reactive-oxygen-species (ROS) levels were determined using enhanced chemiluminescence. <em>F. nucleatum</em> and <em>P. gingivalis</em> as well as a multi-species biofilm (<em>Streptococcus oralis</em>, <em>F. nucleatum</em> and <em>P. gingivalis</em>) were exposed to 1 mg/mL morin for 24 h, planktonic culture and for 7 days, multi-species biofilm. After exposure, the microbial viability (CFU/mL) and biofilm biomass (crystal violet assay) were determined. Live/dead staining and confocal laser scanning microscopy were also performed. Data were submitted to one way ANOVA followed by Tukey's post-hoc test (α=0.05).</div></div><div><h3>Results</h3><div>Morin and the polymer-based formulation containing morin significantly attenuated secretion of GM-CSF, significantly reduced ROS generation and the gene expression levels of the cytokines tested (p &lt; 0.05). Microbial viability and the biofilm biomass significantly reduced (p &lt; 0.05) after treatment with morin and the polymer-based formulation containing morin.</div></div><div><h3>Conclusions</h3><div>Morin showed an anti-inflammatory and antioxidant effect as well as antimicrobial properties against periodontal pathogens. In addition, polymer-based formulation enhanced these effects.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106343"},"PeriodicalIF":2.2,"publicationDate":"2025-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144481445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and morphological analysis of permeable dentin exposed to experimental solutions containing epigallocatechin-3-gallate encapsulated in chitosan nanoparticles","authors":"Davi Ariel Nobuo Bepu ,&nbsp;Karen Pintado-Palomino ,&nbsp;Letícia de Sousa Franco ,&nbsp;Débora Fernandes Costa Guedes ,&nbsp;Luiza Araújo Gusmão ,&nbsp;Antônio Cláudio Tedesco ,&nbsp;Silmara Aparecida Milori Corona","doi":"10.1016/j.archoralbio.2025.106342","DOIUrl":"10.1016/j.archoralbio.2025.106342","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the effects of epigallocatechin 3-gallate (EGCG) and chitosan-based solutions on the chemical and morphological structure of permeable dentin using Fourier Transform Infrared Spectroscopy with Attenuated Reflectance Accessory (FTIR-ATR), Energy dispersive X-ray spectroscopy – EDS, and Scanning Electron Microscopy (SEM) analyses.</div></div><div><h3>Design</h3><div>Bovine root dentin specimens were demineralized with EDTA to simulate dentin hypersensitivity. Specimens were divided into six groups (n = 6): EGCG encapsulated in chitosan nanoparticles (Nchi + EGCG), chitosan nanoparticles (Nchi), EGCG, Elmex Sensitive, deionized water (negative control), and No solution/no acid challenge. Solutions were applied, followed by cycles of citric acid (2 min, 0.3 %, pH 2.45) and artificial saliva (1 h, 4 ×/7 days). Data were analyzed using Kolmogorov-Smirnov, ANOVA, and Tukey’s tests.</div></div><div><h3>Results</h3><div>FTIR spectra revealed enhanced phosphate, carbonate, and amide bands in Nchi + EGCG, EGCG, Nchi, and Elmex groups. EDS showed no significant differences in Ca/P ratios among treated groups, but Nchi and Nchi + EGCG differed significantly from the No solution/no acid challenge (p &lt; 0.05). There were significant differences in the number of dentinal tubules between the Nchi/Nchi + EGCG and EGCG/No solution applied groups (P &lt; 0.05), but no differences among experimental solutions and negative control (p &gt; 0.05). Nchi + EGCG and EGCG showed the smallest open dentinal tubule areas (P = 0.00 and P = 0.001, respectively).</div></div><div><h3>Conclusion</h3><div>Nchi + EGCG modified dentin by enhancing collagen interaction; however, it does not alter Ca/P ratio. Solutions containing EGCG (EGCG and Nchi + EGCG) can reduce the open tubule area.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"178 ","pages":"Article 106342"},"PeriodicalIF":2.2,"publicationDate":"2025-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144490159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of mechanical load on mandibular condylar cartilage and subchondral bone of male rats","authors":"Takahiro Honda,&nbsp;Tomohiro Fukunaga,&nbsp;Arata Ito,&nbsp;Michiko Yoshida,&nbsp;Kozue Yasuno,&nbsp;Hiroka Tsumaki,&nbsp;Kaya Yamaguchi,&nbsp;Yoon Seung Choi,&nbsp;Itaru Mizoguchi","doi":"10.1016/j.archoralbio.2025.106341","DOIUrl":"10.1016/j.archoralbio.2025.106341","url":null,"abstract":"<div><h3>Objective</h3><div>The mandibular condylar cartilage plays significant roles in growth and articular function. This study evaluated the influence of increased joint loading on the growth of the condylar cartilage and the morphology of the mandible.</div></div><div><h3>Design</h3><div>Forty 7-week-old male Wistar rats were assigned to two groups: a control group and an incisal bite plane group. The condylar cartilage and subchondral bone were evaluated via histochemistry, bone histomorphometry with microcomputed tomography (micro-CT), and real-time polymerase chain reaction (PCR) at 2 and 4 weeks. The morphology of the mandible was analyzed via micro-CT.</div></div><div><h3>Results</h3><div>The chondrocytic cell layer became thinner in the posterosuperior region after 2 and 4 weeks of wearing the bite plane. Hypertrophied chondrocytes and calcified cartilage matrix disappeared. Subchondral bone, which runs parallel to the lower border of the chondrocytic cell layer, increased. Bone volume fraction (BV/TV), trabecular thickness (Tb.Th), and trabecular number (Tb.N) increased, while the amount of endochondral bone formation decreased, in the posterosuperior region of the condyle in the bite plane group, which also had reduced mRNA levels of type II collagen, type X collagen, and tartrate-resistant acid phosphatase but increased levels of osteocalcin and alkaline phosphatase. The total mandibular length and ramus height were significantly shorter in the bite plane group, although there was no significant group difference in the length of the mandibular body.</div></div><div><h3>Conclusions</h3><div>The biomechanical environment regulates the cellular organization of the condylar cartilage and bone formation in its subchondral region, which may eventually change mandibular morphology.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106341"},"PeriodicalIF":2.2,"publicationDate":"2025-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dentinogenesis imperfecta, sex, and tooth type on the compositional and structural organization of the dentin-enamel junction in the osteogenesis imperfecta murine model","authors":"Sobhan Katebifar ,&nbsp;Kai Clarke ,&nbsp;Bradley S. Rosenberg ,&nbsp;Michael Truhlar ,&nbsp;Alix C. Deymier","doi":"10.1016/j.archoralbio.2025.106340","DOIUrl":"10.1016/j.archoralbio.2025.106340","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigates the structural and compositional features of the dentin-enamel junction in the osteogenesis imperfecta murine teeth, focusing on the effects of dentinogenesis imperfecta, sex, and tooth type.</div></div><div><h3>Design</h3><div>The study compares dentin-enamel junctions in molars and incisors, as these teeth experience different functions and growth patterns, using a multiscale approach that combines micro-computed tomography (micro-CT), Scanning Electron Microscopy (SEM), and Raman Spectroscopy techniques.</div></div><div><h3>Results</h3><div>The width of the mantle dentin is significantly reduced in dentinogenesis imperfecta-affected mice. Female mice show significantly larger gradients in mineral content and structural dentin-enamel junction than male mice. Molars have larger gradient widths compared to incisors. Molars exhibit higher dentin porosity and a smaller mantle dentin width compared to incisors.</div></div><div><h3>Conclusion</h3><div>Dentinogenesis imperfecta causes a decrease in tubule diameter and an increase in the number of tubules, which may contribute to heightened tooth sensitivity in dentinogenesis imperfecta-affected mice. Females may have better resilience to stress and fractures, potentially explaining why dentinogenesis imperfecta might be diagnosed more frequently in males. Larger gradient widths in molars may help protect them due to their different functions and mechanical stresses.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106340"},"PeriodicalIF":2.2,"publicationDate":"2025-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144471982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TGF-β1 regulates pannexin1 channels and evokes apoptotic response in osteoblasts","authors":"Wenjing Liu","doi":"10.1016/j.archoralbio.2025.106336","DOIUrl":"10.1016/j.archoralbio.2025.106336","url":null,"abstract":"<div><h3>Objective</h3><div>Extracellular ATP is suggested to be involved in cell-cell interactions and TGF-β1 stimulates ATP release through pannexin1 channels. However, the role of TGF-β1 in regulating of pannexin1 channels and cell apoptosis remains unclear. In the present study, the aim was to clarify the role of TGF-β1 in relation to pannexin1 channels and cell apoptosis in osteoblasts.</div></div><div><h3>Design</h3><div>The detection of pannexin1 expression induced by TGF-β1 was achieved using immunofluorescence labeling and western blot analysis. The activity of pannexin1 channels was detected by the dye uptake assay. This study employed the MEK inhibitor U0126 to block ERK signaling in order to investigate the signaling pathway which is involved in the effect of TGF-β1 on pannexin1. In order to determine the expression of cleaved caspase-3 in osteoblasts, immunofluorescence labeling was employed. Flow cytometry was performed to detect the rate of apoptotic cells.</div></div><div><h3>Results</h3><div>Initially, the data of this study showed that TGF-β1 increase the expression of pannexin1 in both primary osteoblasts and the MC3T3 cell line. This study also confirmed that TGF-β1 triggers osteoblast ethidium bromide (EtBr) dye uptake by pannexin1 channels. The inhibition of ERK signaling pathway eliminated TGF-β1’s ability to promote pannexin1. Pannexin 1 is up-regulated in osteoblasts that undergo apoptosis due to high concentration of TGF-β1.</div></div><div><h3>Conclusions</h3><div>According to these findings, high concentration of TGF-β1 up-regulates the expression of pannexin1 and the activation of pannexin1 channels. The ERK signaling pathway mediates this regulation, which induces the apoptosis of osteoblasts.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106336"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144365788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fusion of permanent maxillary incisors of archaeological origin: A case report from late medieval Croatia","authors":"Mario Novak ,&nbsp;Igor Erjavec ,&nbsp;Mario Carić ,&nbsp;Željka Bedić ,&nbsp;Veronika Bencerić ,&nbsp;Josip Burmaz ,&nbsp;Dženi Los ,&nbsp;Mislav Čavka ,&nbsp;Marin Vodanović","doi":"10.1016/j.archoralbio.2025.106339","DOIUrl":"10.1016/j.archoralbio.2025.106339","url":null,"abstract":"<div><h3>Objective</h3><div>The study aims to investigate a case of fusion of the permanent central and lateral right maxillary incisors observed in a middle-aged male from the late medieval town of Sisak in Croatia.</div></div><div><h3>Design</h3><div>“Double teeth” is a term for a developmental anomaly caused by congenital, inherited, acquired and/or idiopathic factors resulting in the union of two adjacent teeth. It usually occurs in three different varieties: concrescence, gemination and fusion. Although there are many reports of such disorders in recent populations, similar examples in archaeological contexts are still rare and, in most cases, recorded in primary dentition. We employed a combination of morphological (macroscopic) and state-of-the-art radiographic analysis (micro-CT scanning).</div></div><div><h3>Results</h3><div>The teeth in question are fused in both coronal and radicular pulp parts, indicating a complete fusion in comparison to the left maxillary incisors that have a normal morphology and are completely separated. The fused RI1 and RI2 have a single crown with a slight groove running along the lingual side of the root which is also visible on the buccal side of the root. Two small protrusions (<em>tuberculum dentale</em>) are visible on the lingual side of the crown. Micro-CT scans confirmed that the fused teeth have a single pulp chamber and root canal.</div></div><div><h3>Conclusions</h3><div>The presented case is unique as it is the first known archaeological specimen of permanent maxillary incisors fusion from Europe and one of the very few known cases of the fusion of permanent teeth from archaeological contexts globally.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106339"},"PeriodicalIF":2.2,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144320861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extracellular matrix protein composition corresponds to degenerative changes in disc displacement of the temporomandibular joint","authors":"Mattias Ulmner ,&nbsp;Rachael Sugars ,&nbsp;Aron Naimi-Akbar ,&nbsp;Bodil Lund","doi":"10.1016/j.archoralbio.2025.106338","DOIUrl":"10.1016/j.archoralbio.2025.106338","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate changes to the composition of a panel of extracellular matrix (ECM) proteins in the temporomandibular joint (TMJ) posterior disc attachment in relation to the diagnoses disc displacement with/without reduction (DDwR, DDwoR).</div></div><div><h3>Design</h3><div>Patients scheduled for arthroscopy or open joint surgery due to DDwR or DDwoR were eligible for inclusion. During surgery biopsies of the posterior disc attachment were harvested. Proteins were extracted from the biopsies and screened using multiplex bead analysis for 23 different ECM proteins. Patients with DDwR served as controls to DDwoR. The variables gender, age, symptom duration, previous trauma, and Wilkes criteria were assessed in relation to the protein concentrations with a quantile regression model. Significance was set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>DDwoR patients had smaller preoperative mouth opening capacity (p &lt; 0.001) and shorter symptom duration (p = 0.007) compared to DDwR. Patients with a sudden onset of DDwoR more often had trauma to the jaws as an inciting event compared to delayed onset DDwoR (p = 0.014). In the adjusted analyses, ADAMTS13 (p = 0.000), aggrecan (p = 0.012), collagen IVα1 (p = 0.001), Lumican (p = 0.008), MMP-7 (p = 0.000), MMP-10 (p = 0.043), NCAM-1 (p = 0.006), Tenascin C (p = 0.030), TIMP-2 (p = 0.000), and TIMP-3 (p = 0.027) had significantly higher concentrations in DDwoR. MMP-9 (p = 0.019) and syndecan-4 (p = 0.002) demonstrated a positive strong correlation to female gender. A higher Wilkes criteria corresponded to a higher protein composition similarly to how protein composition increased in DDwoR compared to DDwR.</div></div><div><h3>Conclusion</h3><div>ECM protein composition changes with TMJ diagnoses and Wilkes criteria suggesting degenerative changes as a main factor. Gender seems to marginally affect the ECM.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106338"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144322118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a liquid-liquid phase separation-related genes signature to predict clinical outcomes, tumor immune microenvironment and chemotherapeutic response in head and neck squamous cell carcinoma","authors":"Pin Lv ,&nbsp;Yang Zhang ,&nbsp;Yuhan Wang,&nbsp;Pengfei Diao,&nbsp;Yaping Wu,&nbsp;Jie Cheng","doi":"10.1016/j.archoralbio.2025.106334","DOIUrl":"10.1016/j.archoralbio.2025.106334","url":null,"abstract":"<div><h3>Objective</h3><div>Liquid-liquid phase separation (LLPS) has been linked to oncogenesis, prompting research into LLPS-related genes as prognostic markers. Here, our objective was to develop a prognostic signature specific to head and neck squamous cell carcinoma (HNSCC) utilizing genes associated with LLPS.</div></div><div><h3>Design</h3><div>The LRG signature was developed using transcriptional sequence data obtained from the TCGA and GEO databases, employing univariate Cox regression, Kaplan-Meier analysis, and LASSO algorithms. Additionally, we developed an LRG nomogram that integrates the signature with specific clinicopathological features, identified using multivariate Cox regression. Clinical value of LRG signature in predicting chemotherapeutic drug sensitivity and immune infiltration was evaluated by bioinformatics approaches. One of the genes in the signature was knocked down to confirm its role <em>in vitro</em>.</div></div><div><h3>Results</h3><div>The LRG signature demonstrated prognostic performance across multiple independent cohorts. Moreover, LRG signature scores exhibited a negative correlation with the presence of tumor-infiltrating immune cells and were associated with the sensitivities of various chemotherapeutic agents. LRP12 silencing markedly inhibited the proliferation, migration, and invasion of HNSCC cells, while also inducing apoptosis in these cells.</div></div><div><h3>Conclusion</h3><div>The findings of our study underscore the efficacy of the LRG signature and nomogram in forecasting prognosis and response to chemotherapy, suggesting a favorable outlook for their application in clinical settings.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106334"},"PeriodicalIF":2.2,"publicationDate":"2025-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144291317","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}