Archives of oral biology最新文献

{"title":"Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography","authors":"Didem Sakaryalı Uyar ,&nbsp;Hazal Karslıoğlu ,&nbsp;Mert Ocak ,&nbsp;Hakan Hamdi Çelik","doi":"10.1016/j.archoralbio.2024.106111","DOIUrl":"10.1016/j.archoralbio.2024.106111","url":null,"abstract":"<div><h3>Objectives</h3><div>To determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth.</div></div><div><h3>Design</h3><div>This study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p &lt; 0.05.</div></div><div><h3>Results</h3><div>There was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p &lt; 0.05) and middle (p = 0.010; p &lt; 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p &lt; 0.001; p &lt; 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p &lt; 0.05).</div></div><div><h3>Conclusion</h3><div>There were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106111"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of dental chipping for identifying and diagnosing tooth fracture patterns in osteological series","authors":"Á. Rubio Salvador ,&nbsp;S.A. Jiménez-Brobeil ,&nbsp;M. Lozano","doi":"10.1016/j.archoralbio.2024.106114","DOIUrl":"10.1016/j.archoralbio.2024.106114","url":null,"abstract":"<div><h3>Objective</h3><div>To develop a specific methodology for identifying dental chipping and determining its temporal occurrence in past populations.</div></div><div><h3>Design</h3><div>The analysed sample comprised of 2191 human teeth from various Bronze Age on the Iberian Peninsula (Argar culture, 1900–1450 cal BC). Among these, 471 chipped teeth were identified. Chipping was examined using various microscopic techniques (digital three-dimensional, optical, and confocal), focusing on distribution, morphology, position in the tooth, extent of damage, and post-chipping antemortem modifications (PCAM).</div></div><div><h3>Results</h3><div>The distribution and morphology of the chips enabled the identification chipping mechanism of the chipping, providing valid criteria to distinguish between antemortem and postmortem chipping. Microscopic analyses of the chipping segments—edges, sidewalls, surface, and surrounding area—facilitated determination of the time the chip ocurred (antemortem: recent, less recent, or not recent).</div></div><div><h3>Conclusions</h3><div>While experimental studies provide valuable insights into chipping mechanisms, many criteria may not be applicable to past populations because of the presence of PCAM. The lack of PCAM in some Argaric teeth suggests that previous studies may have underestimated the prevalence of chipping in past populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106114"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of icariin on dental pulp stem cells and its potential applications in dentin repair","authors":"Ahmed Elhakim ,&nbsp;Ukseong Kim ,&nbsp;Euiseong Kim ,&nbsp;Sukjoon Lee ,&nbsp;Jong-Min Lee ,&nbsp;Han-Sung Jung ,&nbsp;Sunil Kim","doi":"10.1016/j.archoralbio.2024.106112","DOIUrl":"10.1016/j.archoralbio.2024.106112","url":null,"abstract":"<div><h3>Objectives</h3><div>As dental pulp therapy evolves towards regenerative approaches, biomolecules such as icariin, derived from <em>Epimedium</em> flowers, are being evaluated for their therapeutic potential. This study investigates icariin's effectiveness in promoting odontogenic differentiation in human dental pulp stem cells (hDPSCs) <em>in vitro</em> and as a pulp-capping agent <em>in vivo</em>.</div></div><div><h3>Design</h3><div>The study explored the effects of icariin on hDPSCs at concentrations of 10, 20, and 40 µM. Cell viability and migration assays were conducted to evaluate cytotoxicity and chemotaxis. Odontogenic differentiation was assessed using alkaline phosphatase staining and alizarin red S (ARS) staining, complemented by real-time PCR and Western blot analyses of key markers such as RUNX family transcription factor 2 (<em>RUNX2</em>), collagen type I alpha 1 chain (<em>COL1A1</em>), alkaline phosphatase (<em>ALPL</em>), and dentin sialophosphoprotein (<em>DSPP</em>). Additionally, the <em>in vivo</em> effects of icariin were tested in a rat maxillary molar model, where icariin-treated collagen sponges were used for direct pulp capping to evaluate its potential to induce reparative dentin formation.</div></div><div><h3>Results</h3><div>Icariin showed no cytotoxic effects on hDPSCs at any tested concentration, enhanced migratory activity in a dose-dependent manner, and significantly increased alkaline phosphatase activity and calcium deposition. Gene and protein expression analyses revealed a dose-dependent increase in odontogenic differentiation markers in icariin-treated hDPSCs. <em>In vivo</em>, icariin effectively promoted reparative dentin formation in exposed rat pulp.</div></div><div><h3>Conclusions</h3><div>Icariin enhances odontogenic differentiation of hDPSCs and has promising potential as a pulp-capping agent for vital pulp therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106112"},"PeriodicalIF":2.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"DNA damage and cell death in human oral squamous cell carcinoma cells: The potential biological effects of cannabidiol","authors":"Monia Billi ,&nbsp;Stefano Pagano ,&nbsp;Gian Luca Pancrazi ,&nbsp;Chiara Valenti ,&nbsp;Stefano Bruscoli ,&nbsp;Alessandro Di Michele ,&nbsp;Marta Febo ,&nbsp;Francesco Grignani ,&nbsp;Lorella Marinucci","doi":"10.1016/j.archoralbio.2024.106110","DOIUrl":"10.1016/j.archoralbio.2024.106110","url":null,"abstract":"<div><h3>Objective</h3><div>The present study examined the in vitro effects on oral squamous cell carcinoma cells (HSC-3) of cannabidiol (CBD), the main chemical component of Cannabis, proposed as a novel adjuvant therapy in the treatment of cancers.</div></div><div><h3>Design</h3><div>Cell viability (MTT assay), morphology (SEM), apoptosis and cell cycle (flow cytometry), and DNA damage (phospho-γ-H2AX immunofluorescence) were evaluated. Cytotoxicity was evaluated with concentrations between 100 µM and 1 µM, and two concentrations were selected for subsequent analysis: 25 µM, as toxic dose, and 6.25 µM, as non-toxic.</div></div><div><h3>Results</h3><div>CBD caused a dose- and time-dependent reduction in viability of 64 %, 96 %, and 99 % with 25 µM, 50 µM and 100 µM, respectively, after 72 h (p &lt; 0.001), cell cycle arrest in G0-G1 phase with increased apoptosis in particular at 72 h for 25 µM (p &lt; 0.001), significant morphological alterations with 25 µM, still present even at 6.25 µM, and significantly increased cell damage considering a significant increase in the percentage of highly positive cells (5 phosphorylated γH2AX foci), which is around 29 % for 25 µM and 19 % for 6.25 µM after 24 h.</div></div><div><h3>Conclusions</h3><div>CBD inhibits oral cancer growth causing DNA damage. In general, induced cell cytotoxicity appears to be dose- and time-related. Doses of CBD ≥25 μM showed a high reduction in viability. CBD could possibly represent a new therapeutic molecule for its cytotoxic effects against oral squamous cell carcinoma. The mechanism involved in the suppressive effect caused by CBD needs further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106110"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melanoma-inhibiting activity promotes the migration and odontoblastic differentiation of stem cells of apical papilla","authors":"Huihui Ren ,&nbsp;Qingxuan Zhao ,&nbsp;Nan Wang ,&nbsp;Xiaojing Yuan ,&nbsp;Rui Song ,&nbsp;Quan Wen ,&nbsp;Yuming Zhao","doi":"10.1016/j.archoralbio.2024.106109","DOIUrl":"10.1016/j.archoralbio.2024.106109","url":null,"abstract":"<div><h3>Objective</h3><div>Melanoma Inhibitory Activity (MIA) has been predominantly studied in the context of melanoma and cartilage development. However, its role in dental pulp development and stem cell behavior remains largely unexplored. This study investigates the expression pattern of MIA in dental pulp tissues and its potential role in the proliferation, migration, and odontoblastic differentiation of stem cells from the apical papilla (SCAPs).</div></div><div><h3>Design</h3><div>MIA expression in human pulp tissue was demonstrated by immunohistochemistry. SCAPs were cultured in normal and mineralization induction media, with MIA levels monitored via RT-qPCR and Western blot. Cell proliferation was evaluated using the CCK8 assay, while transwell and cell scratch assays were conducted to examine cell migration. The effect of MIA on odontoblastic differentiation was examined by qRT-PCR, Alkaline phosphatase activity assay, and Western blot. siRNA was used to knock down MIA to investigate its effect. A mouse subcutaneous implantation model was used to assess whether MIA promotes odontoblastic differentiation <em>in vivo</em>.</div></div><div><h3>Results</h3><div>MIA expression was observed in the papilla and odontoblasts layer of the developing pulp. <em>In vitro</em>, MIA expression increased during SCAPs differentiation and was found to significantly enhance migration, and odontoblastic differentiation but not proliferation. Gene knockdown experiments confirmed MIA’s pivotal role in promoting SCAPs migration and differentiation. <em>In vivo</em>, MIA facilitated the formation of dentin-like structures and enhanced pulp-dentin complex regeneration.</div></div><div><h3>Conclusion</h3><div>MIA plays a crucial role in SCAPs’ migration and differentiation, suggesting its potential application in pulp-dentin regeneration therapies. Further studies are required to fully elucidate the underlying mechanisms.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106109"},"PeriodicalIF":2.2,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vitamin D serum levels and temporomandibular disorders: A systematic review and meta-analysis","authors":"Reza Tabrizi ,&nbsp;Hooman Khanzadeh ,&nbsp;Seyed Sepehr Mirebeigi Jamasbi ,&nbsp;Fatemeh Rezaei ,&nbsp;Ali Azadi","doi":"10.1016/j.archoralbio.2024.106108","DOIUrl":"10.1016/j.archoralbio.2024.106108","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review evaluates the connection between vitamin D serum levels, deficiency, and temporomandibular disorders (TMD), offering a meta-analysis of the existing evidence in this domain.</div></div><div><h3>Design</h3><div>The Scopus, ISI Web of Science, and Pubmed databases were searched for human studies concerning the connection between vitamin D and TMD comprising a control group. A random-effect model with forest plots was used for vitamin D serum levels mean difference (MD), vitamin D deficiency odds ratio (OR), and risk difference (RD) between subjects with and without TMD. Subgroup analysis was conducted based on ethnicity, overall risk of bias, TMD diagnosis method, and study designs. A p-value lower than 0.05 was considered significant. The certainty of the meta-evidence was evaluated according to the GRADE approach.</div></div><div><h3>Results</h3><div>Of the 2621 identified unique records, 15 studies were included in the study, eight of which were considered for the meta-analysis. The meta-analysis revealed a significant vitamin D deficiency OR (3.85; 95 % CI: 2.35 – 5.43; Certainty: Low) and RD (22 %; 95 % CI: 11 % - 32 %; Certainty: Very low), and vitamin D serum levels MD (-5.03 ng/mL; 95 % CI: −9.92 – −0.13; Certainty: Very low) between subjects with and without TMD. Among subgroup analyses, only the difference in vitamin D MD between Middle Eastern and European patients was significant (<em>P</em> &lt; 0.01).</div></div><div><h3>Conclusion</h3><div>Considering the low to very low certainty of the evidence, vitamin D serum levels are significantly lower, and vitamin D deficiency is significantly more prevalent in TMD patients.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106108"},"PeriodicalIF":2.2,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514516","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interaction between polymorphisms in TNF-⍺ and RANKL genes is associated with the development of persistent apical periodontitis, in Brazilian subjects","authors":"Igor Bassi Ferreira Petean ,&nbsp;Alice Corrêa Silva-Sousa ,&nbsp;Guido Artemio Marañón-Vásquez ,&nbsp;Francisco Wanderley Garcia de Paula-Silva ,&nbsp;Erika Calvano Küchler ,&nbsp;Leonardo Santos Antunes ,&nbsp;Raquel Assed Bezerra Segato ,&nbsp;Lea Assed Bezerra da Silva ,&nbsp;Jardel Francisco Mazzi-Chaves ,&nbsp;Fabiane Carneiro Lopes-Olhê ,&nbsp;Manoel Damião Sousa-Neto","doi":"10.1016/j.archoralbio.2024.106106","DOIUrl":"10.1016/j.archoralbio.2024.106106","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the association between genetic polymorphisms in suppressor of cytokine signaling-1 (<em>SOCS-1</em>)<em>,</em> tumor necrosis factor-⍺ (<em>TNF-α</em>) and its receptors 1 and 2 (<em>TNFRSF1A</em> and <em>TNFRSF1B</em>), receptor activator of nuclear factor kappa-b (<em>RANK</em>), receptor activator of nuclear factor-kappa B ligand (<em>RANKL</em>) and osteoprotegerin (<em>OPG</em>), and persistent apical periodontitis (PAP).</div></div><div><h3>Methods</h3><div>Patients with pulp necrosis and apical periodontitis at the time of non-surgical root canal treatment were followed up for at least one year. A total of 423 subjects were included, 172 with signs/symptoms of PAP and 251 with apical periodontitis healed. DNA was extracted from saliva and used for genotyping polymorphisms in <em>SOCS1</em> (rs243327 and rs33977706), <em>TNF-α</em> (rs1800629), <em>TNFRSF1A</em> (rs1800693), <em>TNFRSF1B</em> (rs1061622), <em>RANK</em> (rs1805034), <em>RANKL</em> (rs1054016) and <em>OPG</em> (rs1032128) by real-time PCR. The frequency of genotypes and alleles was assessed using chi-squared test or Fisher's exact test and odds ratio. Interactions were also tested using multifactor dimensionality reduction (<em>α</em> = 5 %).</div></div><div><h3>Results</h3><div>In the polymorphism rs1800629 in <em>TNF-</em>α, carrying at least one A risk allele significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.28–0.79; <em>p</em>=0.004). For the polymorphism rs1054016 in <em>RANKL</em> carrying both T risk alleles significantly decreased the risk to develop PAP (OR=0.47; 95 %CI: 0.24–0.92; <em>p</em>=0.027). None of the other polymorphisms evaluated were associated with PAP (<em>p</em>&gt;0.05). A strong interaction was observed among rs1800629, rs1061622 and rs1054016.</div></div><div><h3>Conclusions</h3><div>Genetic polymorphisms rs1800629 (<em>TNF-α)</em> and rs1054016 (<em>RANKL)</em> had a strong interaction and were associated with a lower risk to develop PAP.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106106"},"PeriodicalIF":2.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial and antibiofilm potential of Thuja orientalis L. extract targeting cariogenic Enterococcus faecalis ATCC 29212: A combined in-vitro, in-silico study, and cytotoxicity screening","authors":"Khyati Koul ,&nbsp;Ishwerpreet Kaur Jawanda ,&nbsp;Thomson Soni ,&nbsp;Kashish Madaan ,&nbsp;Sunidhi Bhatt ,&nbsp;Pranjali Singh ,&nbsp;Divyani Sharma ,&nbsp;Sonia Bhonchal Bhardwaj ,&nbsp;Seema Kumari","doi":"10.1016/j.archoralbio.2024.106107","DOIUrl":"10.1016/j.archoralbio.2024.106107","url":null,"abstract":"<div><h3>Objectives</h3><div>In this study, we explored the efficacy of methanolic extract of <em>Thuja orientalis</em> (TOME) as a novel antibacterial and antibiofilm agent against a cariogenic bacterium, <em>Enterococcus faecalis</em> ATCC 29212.</div></div><div><h3>Design</h3><div>Antibacterial susceptibility studies were conducted and surface morphology analysis was performed using field emission scanning electron microscopy (FESEM). Antibiofilm activity was evaluated through both qualitative and quantitative biofilm inhibition assays and validated by microscopic analysis. <em>In-silico</em> molecular docking studies were conducted using the EDock server. The effectiveness of TOME was substantiated by biofilm model on dentin discs and cytotoxicity towards the HaCaT cell line was assessed using the MTT assay.</div></div><div><h3>Results</h3><div>TOME exhibited significant bactericidal activity with minimum inhibitory concentration of 12.5 mg/mL and additionally, it effectively compromised bacterial cell wall integrity. Qualitative, quantitative and microscopic studies depicted the inhibition of biofilm formation. TOME significantly impacted the production of extracellular polymeric substance and extracellular DNA. Molecular docking studies identified beta-caryophyllene as a potent inhibitor of the Enterococcal surface protein (Esp). Biofilm model depicted the reduction of bacterial load on dentin discs. Additionally, TOME showed reduced cytotoxicity on HaCaT cells, indicating its potential as a safe therapeutic agent.</div></div><div><h3>Conclusion</h3><div>These findings highlight TOME's promise for developing novel treatments for dental infections and biofilm-associated diseases. Further research should focus on isolating and characterizing the active compounds within TOME, particularly beta-caryophyllene, to elucidate their precise mechanisms of action.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106107"},"PeriodicalIF":2.2,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142796736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Masticatory function and three-dimensional facial morphology of soft tissues: One year after orthognathic surgery","authors":"Joana Carolina Martins Simões ,&nbsp;Denny Marcos Garcia ,&nbsp;Francisco Veríssimo De Mello-Filho ,&nbsp;Claudia Maria De Felício ,&nbsp;Luciana Vitaliano Voi Trawitzki","doi":"10.1016/j.archoralbio.2024.106103","DOIUrl":"10.1016/j.archoralbio.2024.106103","url":null,"abstract":"<div><h3>Objective</h3><div>Dentofacial deformities (DFD) require orthodontic treatment, orthognathic surgery, and speech therapy for aesthetic and functional problems. This longitudinal study analyzed changes in masticatory function and three-dimensional (3D) facial soft tissue in patients with Class II and Class III DFD after orthognathic surgery. In addition, the study investigated the relationship between facial measurements, maximum bite force (MBF), and orofacial myofunctional status (OMS).</div></div><div><h3>Design</h3><div>The sample consisted of 46 participants, including 10 patients with Class II DFD, and 11 patients with Class III DFD. These groups were assessed before (T0) and 6 months (T1) after surgery. Twelve patients who completed the treatment protocol were evaluated 12 months post-surgery (T2). The patient groups were compared with each other and with a control group (CG) of 25 healthy subjects. The participants underwent MBF and clinical evaluation of OMS, including masticatory behavior, using a validated protocol. The 3D facial soft tissue was assessed using laser scanning.</div></div><div><h3>Results</h3><div>Compared to the CG, DFD patients showed reduced MBF, masticatory behavior, and OMS scores at T0. At T2, there was an improvement in MBF, masticatory, and OMS scores, but differences were observed compared to the CG. Changes in facial soft tissue followed the underlying hard tissue movement resulting from surgery, and reduction of vertical measurements contributed to improvements in masticatory function.</div></div><div><h3>Conclusion</h3><div>These findings indicated that combined surgery, orthodontic treatment, and speech therapy can lead to a significant improvement in masticatory function and facial soft tissue in DFD patients, although not completely 12 months post-surgery.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106103"},"PeriodicalIF":2.2,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intriguing astrocyte responses in CA1 to reduced and rehabilitated masticatory function: Dorsal and ventral distinct perspectives in adult mice","authors":"Micaele Maria Lopes Castro ,&nbsp;Fabio Leite do Amaral Junior ,&nbsp;Fabíola de Carvalho Chaves de Siqueira Mendes ,&nbsp;Daniel Clive Anthony ,&nbsp;Dora Maria Tuna de Oliveira Brites ,&nbsp;Cristovam Wanderley Picanço Diniz ,&nbsp;Marcia Consentino Kronka Sosthenes","doi":"10.1016/j.archoralbio.2024.106097","DOIUrl":"10.1016/j.archoralbio.2024.106097","url":null,"abstract":"<div><h3>Objective</h3><div>We sought to investigate the plasticity of diet-induced changes in astrocyte morphology of <em>stratum lacunosum-moleculare (SLM)</em> in CA1.</div></div><div><h3>Design</h3><div>Three diet regimes were adopted in 15 mice, from the 21st postnatal day to 6 months. The first diet regimen was pellet feed, called Hard Diet (HD). The second, with reduced masticatory, received a pellet-diet followed by a powdered-diet, and it was identified as Hard Diet/Soft Diet (HD/SD). Finally, the group with rehabilitated masticatory was named Hard Diet/Soft Diet/Hard Diet (HD/SD/HD). In the end, euthanasia and brain histological processing were performed, in which astrocytic immunoreactivity to glial-fibrillary-acidic-protein (GFAP) was tested. In reconstructed astrocytes, morphometric analysis was performed.</div></div><div><h3>Results</h3><div>Astrocyte morphometric revealed that changes in masticatory regimens impact astrocyte morphology. In the dorsal CA1, switching from a hard diet to a soft diet led to reductions in most variables, whereas in the ventral, fewer variables were affected, highlighting regional differences in astrocyte responses. Cluster analysis further showed that diet-induced changes in astrocyte morphology were reversible in the dorsal region, but not in the ventral region, indicating a persistent impact on astrocyte diversity and complexity in the ventral even after rehabilitation. Correlation tests between astrocyte morphology and behavioral performance demonstrated disrupted relationships under masticatory stress, with effects persisting after rehabilitation.</div></div><div><h3>Conclusion</h3><div>Changes in the diet result in significant alterations in astrocyte morphology, suggesting a direct link between dietary modulation and cellular structure. Morphometric analyses revealed distinct alterations in astrocyte morphology in response to changes in the masticatory regimen, with both dorsal/ventral regions displaying notable changes. Moreover, the regional differential effects on astrocytes underscore the complexity of mastication on neuroplasticity and cognitive function.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106097"},"PeriodicalIF":2.2,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142423711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}