Archives of oral biology最新文献

{"title":"Morphological traits and caries susceptibility of the cusp of carabelli in permanent maxillary molars: A study in Lahore, Pakistan","authors":"Maliha Shahbaz ,&nbsp;Naauman Zaheer ,&nbsp;Usman Zaheer ,&nbsp;Madiha ,&nbsp;Muhammad Haris Bilal ,&nbsp;Abdullah Sajid ,&nbsp;Junaid Ali ,&nbsp;Hareem Aziz ,&nbsp;Khurram Nadeem","doi":"10.1016/j.archoralbio.2025.106406","DOIUrl":"10.1016/j.archoralbio.2025.106406","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate the prevalence, morphological traits, and caries susceptibility of the Cusp of Carabelli (CoC) in permanent maxillary molars among patients in Lahore, Pakistan.</div></div><div><h3>Design</h3><div>A descriptive cross-sectional study was conducted among 432 participants aged 12 years or older at Lahore Medical and Dental College. Clinical examination of maxillary first and second molars was performed using the Arizona State University Dental Anthropology System (ASUDAS) for CoC traits and the International Caries Detection and Assessment System (ICDAS) for caries assessment. Inter- and intra-examiner calibration ensured diagnostic reliability (Cohen's Kappa &gt;0.90).</div></div><div><h3>Results</h3><div>CoC (ASUDAS grades 1–7) was observed in 201 individuals (46.5 %) on maxillary first molars and in 7 individuals (1.6 %) on second molars, with bilateral expression more common than unilateral. The right first molar showed a higher prevalence of CoC and caries incidence. Morphological traits ranged from subtle grooves to pronounced cusps, with small vertical grooves (ASUDAS 1) being the most frequent. Caries susceptibility correlated positively with CoC prominence (p &lt; 0.001). Multivariate logistic regression identified CoC grade as the strongest predictor of caries, overshadowing age, side, and molar position, and substantially improving model sensitivity (0 % to 97.2 %).</div></div><div><h3>Conclusion</h3><div>CoC is a prevalent trait and is significantly associated with early-stage dental caries in maxillary first molars. Its presence, particularly in prominent forms, may pose an increased risk of caries. These findings underscore the need for enhanced preventive strategies and clinical attention in individuals with CoC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106406"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial on the special issue on temporomandibular disorders (TMD), AOB journal","authors":"Meiqing Wang ,&nbsp;Dongmei He","doi":"10.1016/j.archoralbio.2025.106401","DOIUrl":"10.1016/j.archoralbio.2025.106401","url":null,"abstract":"","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106401"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145120437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunohistochemical assessment of murine double minute 2 in solid vs unicystic ameloblastoma: A systematic review and meta-analysis","authors":"Lilibeth-Stephania Escoto-Vasquez ,&nbsp;Mario Alberto Alarcón-Sánchez ,&nbsp;Julieta Sarai Becerra-Ruiz ,&nbsp;Cristina Hermila Martínez-Bugarin ,&nbsp;Sarah Monserrat Lomelí-Martínez ,&nbsp;Armen A. Muradyan ,&nbsp;Artak Heboyan","doi":"10.1016/j.archoralbio.2025.106400","DOIUrl":"10.1016/j.archoralbio.2025.106400","url":null,"abstract":"<div><h3>Objective</h3><div>This project aimed to evaluate the immunoexpression pattern of murine double minute 2 (MDM2) in solid ameloblastomas compared to unicystic ameloblastomas.</div></div><div><h3>Methods</h3><div>The review followed PRISMA guidelines and was registered in the PROSPERO database. PubMed, Scopus, ScienceDirect, Web of Science, and Google Scholar were comprehensively searched. Original cross-sectional studies were included. The meta-analysis was performed using STATA V15 and RevMan. Positivity rates were pooled using a random-effects model (REM), the labeling index was analyzed using mean difference under a REM, and expression intensity (moderate–strong) was assessed as categorical data using a REM with Hartung-Knapp adjustment. Heterogeneity was evaluated with the Chi² test and I² statistic. The methodological quality and certainty of the evidence were assessed using the Joanna Briggs Institute items and the GRADE system.</div></div><div><h3>Results</h3><div>Nine studies (n = 438 specimens) were analyzed, of which 325/438 (74.2 %) were ameloblastoma biopsies. MDM2 positivity was detected in 403/438 cases (92 %). A statistically significant association in favor of solid ameloblastoma was observed (RR = 2.08; 95 %CI [1.66–2.60]; p = 0.005), indicating a twofold probability of finding high MDM2 expression in solid compared to unicystic ameloblastomas. Four of the nine studies (44.4 %) were considered to be of low quality, and the certainty of evidence was low to very low.</div></div><div><h3>Conclusions</h3><div>MDM2 expression was prevalent in both types of ameloblastomas, with a higher intensity of expression observed in solid cases. However, due to study heterogeneity, further investigations with more robust methodological designs are recommended to assess the diagnostic potential of MDM2 in ameloblastomas.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106400"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145109677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucose as a biomarker in oral potentially malignant disorders and oral squamous cell carcinoma: A systematic review and meta-analysis","authors":"Shruti Gupta ,&nbsp;Deepti Sharma ,&nbsp;Anita Hooda ,&nbsp;Mala Kamboj","doi":"10.1016/j.archoralbio.2025.106423","DOIUrl":"10.1016/j.archoralbio.2025.106423","url":null,"abstract":"<div><h3>Objectives</h3><div>This systematic review and meta-analysis aimed to investigate the potential of fucose as a biomarker for oral potentially malignant disorders (OPMDs) and oral squamous cell carcinoma (OSCC).</div></div><div><h3>Design</h3><div>Relevant articles were searched across multiple databases, including PubMed, Scopus, Google Scholar, Science Direct, and Clinical Key. Twenty-three studies that met the eligibility criteria were included for qualitative review; out of which, 20 studies that provided data for comparison between study and control groups were further subjected to meta-analysis.</div></div><div><h3>Results</h3><div>The analysis revealed significantly high fucose levels in the saliva of OPMD patients (p = 0.013) and OSCC patients (p = 0.003) compared to healthy controls. Additionally, fucose levels were elevated in the serum of patients with OSCC (p &lt; 0.001) and oral leukoplakia (p = 0.027) compared to controls.</div></div><div><h3>Conclusion</h3><div>The findings of this meta-analysis suggest that fucose could serve as a potential biomarker for OPMDs and OSCC. However, the presence of significant heterogeneity in serum evaluation and the limited studies focusing on saliva indicate the need for further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106423"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ZBP1-NLRP3 axis integrates PANoptosis and ferroptosis during inflammatory injury in human dental pulp fibroblasts","authors":"Ai-E. He ,&nbsp;Xing Wang ,&nbsp;Ni Xie,&nbsp;Yun-He Xiao","doi":"10.1016/j.archoralbio.2025.106398","DOIUrl":"10.1016/j.archoralbio.2025.106398","url":null,"abstract":"<div><h3>Objective</h3><div>To define how Z-DNA binding protein 1 (ZBP1) and NOD-like receptor family pyrin domain-containing 3 (NLRP3) signaling regulate lipopolysaccharide (LPS)-induced inflammation, PANoptosis, and ferroptosis in human dental pulp fibroblasts (HDPFs).</div></div><div><h3>Design</h3><div>HDPFs were treated with LPS, and <em>ZBP1</em> and <em>NLRP3</em> were silenced using small interfering RNA (siRNA), individually or in combination. Inflammatory mediators and death-pathway markers were quantified by quantitative real-time PCR (qRT-PCR), Western blotting, enzyme-linked immunosorbent assay (ELISA), and biochemical assays; Annexin V/propidium iodide flow cytometry assessed cell-death distributions.</div></div><div><h3>Results：</h3><div>LPS significantly increased ZBP1 and NLRP3 expression and elevated cytokine/chemokine release; each was attenuated by <em>ZBP1</em> or <em>NLRP3</em> knockdown, with the greatest reduction after dual silencing. LPS triggered PANoptosis, as indicated by increased Annexin V⁺/PI⁺ cell populations and upregulation of caspase-1, cleaved caspase-8, RIPK3, GSDMD, and p-MLKL/MLKL, which were significantly reduced by inhibition of the ZBP1-NLRP3 axis. Ferroptosis features were also evident after LPS, including impaired iron homeostasis (downregulated ferritin heavy chain 1 [FTH1] and ferroportin [FPN1] with Fe²⁺ accumulation), enhanced lipid peroxidation (upregulated ALOX15, LPCAT3, PTGS2 with increased malondialdehyde and lipid reactive oxygen species), and weakened antioxidant defenses (reduced glutathione peroxidase-4 [GPX4], solute carrier family 7 member 11 [SLC7A11], glutathione, and GPX4 activity). These changes were mitigated by single-gene silencing and most effectively by dual knockdown.</div></div><div><h3>Conclusion</h3><div>The ZBP1-NLRP3 axis acts upstream to coordinate LPS-induced PANoptosis and ferroptosis in HDPFs. Targeting this axis dampens inflammatory cell death and oxidative-metabolic dysregulation, highlighting a potential therapeutic strategy for pulpitis-related tissue injury.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106398"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tooth loss induces cognitive decline independent of low-protein diet intake in male mice","authors":"Rie Hatakeyama ,&nbsp;Hiroshi Oue ,&nbsp;Miyuki Yokoi ,&nbsp;Eri Ishida ,&nbsp;Takayasu Kubo ,&nbsp;Kazuhiro Tsuga","doi":"10.1016/j.archoralbio.2025.106421","DOIUrl":"10.1016/j.archoralbio.2025.106421","url":null,"abstract":"<div><h3>Objectives</h3><div>Previous articles have shown that tooth loss is associated with cognitive decline in animal and human studies. Additionally, poor nutritional status including low-protein diet is associated with dementia. The evidence of the association of tooth loss and nutritional status is uncertain. The purpose of this study was to investigate the relationship between low-protein diet intake and cognitive decline following tooth loss in mice.</div></div><div><h3>Design</h3><div>Male senescence-accelerated mouse-prone 8 mice were randomly allocated into sham-operated control and tooth loss groups with extracted maxillary molars: control with normal-protein diet; control with low-protein diet; bilateral maxillary molar extraction with normal-protein diet; and bilateral maxillary molar extraction with low-protein diet. After 6 months, a behavioral test was conducted, and mRNA expression and immunohistochemistry in the brain were analyzed.</div></div><div><h3>Results</h3><div>Behavioral test revealed no effect of the interaction between tooth loss and low-protein diet intake on cognitive decline; however, tooth loss had a marked effect on cognitive decline. Real-time polymerase chain reaction showed no interaction between tooth loss and low-protein intake for <em>Bax/Bcl-2</em> mRNA expression; however, tooth loss had a significant effect on <em>Bax/Bcl-2</em> mRNA expression. Immunohistochemical analysis showed that the effect of tooth loss in neuronal inflammation and neuronal loss were observed in CA1 and DG region, but the effect of low-protein diet was limited in CA3.</div></div><div><h3>Conclusion</h3><div>The present study revealed that the impact of tooth loss on cognitive decline was not dependent on the low-protein diet condition.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106421"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145310278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OIP5-AS1 expression profiles in different stages of oral squamous cell carcinoma","authors":"Ameya K.P.,&nbsp;Ashikha Shirin Usman P.P.,&nbsp;Durairaj Sekar","doi":"10.1016/j.archoralbio.2025.106403","DOIUrl":"10.1016/j.archoralbio.2025.106403","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the expression patterns of long non-coding RNA OIP5-AS1 across various stages of oral squamous cell carcinoma (OSCC) and assess its potential as a biomarker and therapeutic target.</div></div><div><h3>Design</h3><div>A comprehensive review of recent literature focusing on OIP5-AS1's role in OSCC was conducted. Analysis included OIP5-AS1 expression levels in cancerous versus non-cancerous tissues and exploration of its interactions with tumor-suppressing microRNAs (miRNAs).</div></div><div><h3>Results</h3><div>OIP5-AS1 was found to be significantly upregulated in advanced stages of OSCC compared to non-cancerous tissues. Its function as a molecular sponge for miRNAs contributes to the promotion of tumorigenic pathways, complicating therapeutic responses and highlighting its role as an oncogene.</div></div><div><h3>Conclusions</h3><div>OIP5-AS1 is a critical player in the progression of OSCC, influencing tumor dynamics and mechanisms of resistance. Elucidating its expression patterns and functional roles suggests that OIP5-AS1 could serve as a valuable biomarker for early diagnosis and personalized treatment strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106403"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145156632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro antibacterial activity of Lactiplantibacillus sp. cell-free supernatants against Porphyromonas gingivalis: A potential approach for oral health","authors":"Hui Ying Yit ,&nbsp;Zaleha Shafiei ,&nbsp;Noraziah Mohamad Zin ,&nbsp;Mazlina Mohd Said","doi":"10.1016/j.archoralbio.2025.106399","DOIUrl":"10.1016/j.archoralbio.2025.106399","url":null,"abstract":"<div><h3>Objective</h3><div>This study explores the potential of postbiotic metabolites from lactic acid bacteria (LAB) isolated from human milk and tempeh against <em>Porphyromonas gingivalis</em>, a key pathogen in periodontitis.</div></div><div><h3>Design</h3><div>Selected LAB strains and their treated cell-free supernatants (TCFS), lyophilized TCFS (L-TCFS), and bacteriocin-like inhibitory substances (BLIS) were tested individually and in combination using agar well diffusion, MIC, MBC, autoaggregation, coaggregation, and biofilm inhibition assays. LAB identification was performed using the API 50 CHL kit and 16S rDNA sequencing.</div></div><div><h3>Results</h3><div>Strain S2, a mixture of <em>Lactiplantibacillus</em> sp. SUK1 and T2 showed the highest inhibitory activity (20.06 ± 4.14 mm) using the agar well diffusion method. It demonstrated strong autoaggregation compared to the individual T2 strain but lacked significant coaggregation ability. The L-TCFS of S2 also exhibited the strongest antibacterial effect, with a minimum inhibitory concentration (MIC) of 50 mg/mL, although no minimum bactericidal concentration (MBC) was detected for any bacteriocin-like inhibitory substances (BLIS). L-TCFS from strain S2 significantly reduced <em>P. gingi</em>valis biofilm formation at a minimum concentration of 25 mg/mL, compared to the untreated control. Strain T2 was identified as <em>Lactiplantibacillus plantarum</em> using the API 50CHL test and 16S rDNA gene sequencing.</div></div><div><h3>Conclusion</h3><div>The combination metabolite S2 demonstrates promising inhibitory activity against <em>P. gingivalis</em> and may serve as an effective adjunctive therapy for periodontal infections, outperforming individual and other combination LAB strains tested.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106399"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145218424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distinct oral microbiome profiles in substance users in the high-incidence Mizo tribal community, Northeast India","authors":"Lalfak Zuali ,&nbsp;Lalchhanhima Ralte ,&nbsp;John Zothanzama ,&nbsp;Christine Vanlalbiakdiki Sailo ,&nbsp;Nachimuthu Senthil Kumar","doi":"10.1016/j.archoralbio.2025.106418","DOIUrl":"10.1016/j.archoralbio.2025.106418","url":null,"abstract":"<div><h3>Objectives</h3><div>To characterize the oral microbiome of substance users (primarily tobacco, alcohol, and opioids) compared to healthy controls in the high-incidence Mizo tribal community of Northeast India, and to examine their association with food and lifestyle habits.</div></div><div><h3>Methods</h3><div>Saliva samples from 53 substance users and 35 healthy controls were subjected to 16S rRNA (V3–V4 region) sequencing. Alpha and beta diversity analyses, differential abundance testing, and microbial functional prediction were conducted. Statistical analyses were corrected for multiple comparisons using false discovery rate (FDR) adjustment where applicable. Associations with food and lifestyle habits were also examined.</div></div><div><h3>Results</h3><div>Substance users exhibited significantly lower alpha diversity, and beta diversity analyses revealed distinct clustering between the two groups. Firmicutes_D, Actinobacteria, <em>Rothia</em>, and <em>Streptococcus</em> were more abundant in substance users, whereas Bacteroidota, Proteobacteria, <em>Neisseria</em>, and <em>Prevotella</em> were enriched in healthy controls. Functional predictions indicated upregulation of biofilm formation and xenobiotic degradation pathways in substance users. Exposure to Jhum cultivation, prenatal smoking, and consumption of fermented pork fat (saum) were correlated with microbial composition.</div></div><div><h3>Conclusions</h3><div>Substance use and associated environmental exposures were linked to oral microbiome dysbiosis. Certain bacterial taxa may serve as potential microbial biomarkers of substance use in this high-risk tribal population, warranting further investigation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106418"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145304953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression patterns of desmosome family members during tooth development and the role of Desmocollin-3 in cytodifferentiation of stratum intermedium","authors":"Triana Marchelina ,&nbsp;Yuta Chiba ,&nbsp;Shinji Otake ,&nbsp;Li Wanshu ,&nbsp;Hiroshi Sato ,&nbsp;Yumiko Nakashima ,&nbsp;Asuna Sugimoto ,&nbsp;Tsutomu Iwamoto ,&nbsp;Aya Yamada ,&nbsp;Kan Saito ,&nbsp;Satoshi Fukumoto","doi":"10.1016/j.archoralbio.2025.106404","DOIUrl":"10.1016/j.archoralbio.2025.106404","url":null,"abstract":"<div><h3>Objective</h3><div>Desmocollin-3 (Dsc3), a desmosomal cadherin, is critical in maintaining epithelial cohesion and integrity. Despite its recognized function in skin and mucosal epithelium, its contribution to tooth development remains poorly understood. The study aims to identify stratum intermedium (SI)-specific markers using single-cell RNA-sequence (scRNA-seq) and to investigate the functional role of Dsc3 in maintaining SI cell integrity and differentiation.</div></div><div><h3>Design</h3><div>In this study, we pursued the marker genes of SI cells using scRNA-seq analysis of post-natal day 12 molar. Furthermore, we examined the role of the SI marker gene using dental epithelial cell line SF2.</div></div><div><h3>Results</h3><div>We found that desmosome family genes are highly expressed in SI cluster and among them, Dsc3 showed specific expression in SI cluster. Knockdown of Dsc3 in the SF2 epithelial cell line led to significantly smaller cell size, indicating impaired epithelial differentiation. The expression of SI marker genes was suppressed by the knockdown of Dsc3 with a marked loss of tight junction protein Tjp1 (ZO-1), indicating disrupted intercellular junctions and impaired epithelial barrier function. This disruption correlated with altered expression of key ameloblast differentiation markers, suggesting a failure in proper ameloblast lineage commitment, highlighting a disruption in the SI’s ability to support ameloblast lineage specification.</div></div><div><h3>Conclusion</h3><div>These findings indicate that Dsc3 is essential for SI structural integrity and its signaling support to ameloblasts.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106404"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}