Gabriel Pereira Nunes , Renata de Oliveira Alves , Matheus Henrique Faccioli Ragghianti , Alexandre Henrique dos Reis-Prado , Priscila Toninatto Alves de Toledo , Tamires Passadori Martins , Ana Paula Miranda Vieira , Geórgia Rondó Peres , Cristiane Duque
{"title":"Effects of quercetin on mineralized dental tissues: A scoping review","authors":"Gabriel Pereira Nunes , Renata de Oliveira Alves , Matheus Henrique Faccioli Ragghianti , Alexandre Henrique dos Reis-Prado , Priscila Toninatto Alves de Toledo , Tamires Passadori Martins , Ana Paula Miranda Vieira , Geórgia Rondó Peres , Cristiane Duque","doi":"10.1016/j.archoralbio.2024.106119","DOIUrl":"10.1016/j.archoralbio.2024.106119","url":null,"abstract":"<div><h3>Objective</h3><div>This scoping review (SR) aimed to investigate the impact of quercetin on mineralized dental tissues intended to be used in preventive and restorative dentistry.</div></div><div><h3>Methods</h3><div>This SR was conducted following the PRISMA-ScR statement. A comprehensive search was performed across databases for articles published up to March 2024. Eligible studies included <em>in vitro</em> and <em>in situ</em> studies and evaluating the potential therapeutic effects of quercetin on dental enamel and dentin. Data were extracted, and synthesis of study findings was conducted.</div></div><div><h3>Results</h3><div>Out of the 2322 records screened, 22 studies were included in the review. Quercetin, in solution or into dental materials increased the bond strength to enamel and dentin. Additionally, quercetin also enhanced the bond strength of enamel after bleaching. Co-administration of quercetin with fluoride prevented erosive wear and inhibited the proteolytic activity in dentin more effectively than either agent alone. Hardness and modulus of elasticity was higher in dentin treated with quercetin compared to placebo. Reduction of nanoleakage at the composite-dentin interface was reduced in the presence of quercetin as a solution or incorporated into dental adhesives.</div></div><div><h3>Conclusions</h3><div>Quercetin exhibits promising therapeutic effects on mineralized dental tissues, including remineralization and enhancement of bond strength. It shows potential as a multifunctional agent for improving the longevity and effectiveness of dental biomaterials, as well as in preventing erosion and dental caries. However, as these conclusions are largely drawn from lab-based (<em>in vitro</em>) studies, further research, including clinical trials, is needed to fully explore its therapeutic potential and applications in dentistry.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106119"},"PeriodicalIF":2.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Allyl isothiocyanate suppressed periodontal tissue destruction in mice via bacteriostatic and anti-inflammatory activities against Porphyromonas gingivalis","authors":"Yukako Minato, Yukari Aoki-Nonaka, Hnin Yu Lwin, Daiki Ando, Yuko Warita, Aoi Matsugishi-Nasu, Takumi Hiyoshi, Naoki Takahashi, Koichi Tabeta","doi":"10.1016/j.archoralbio.2024.106118","DOIUrl":"10.1016/j.archoralbio.2024.106118","url":null,"abstract":"<div><h3>Objectives</h3><div>Allyl isothiocyanate (AITC) is a phytochemical that is abundantly present in cruciferous vegetables, such as wasabi and mustard. Among its pharmacological properties, it demonstrates anticancer, antifungal, and anti-inflammatory activities. This study aimed to investigate the functions of AITC against periodontopathic bacteria and its effects on a mouse model of periodontitis.</div></div><div><h3>Design</h3><div>The antimicrobial and antibiofilm functions of AITC were assessed against <em>Porphyromonas gingivalis, Fusobacterium nucleatum,</em> and <em>Streptococcus mitis</em>. To clarify its anti-inflammatory effects, macrophage-like cells from THP-1 were stimulated with <em>P. gingivalis</em> lipopolysaccharide (LPS), and the release of inflammatory cytokines was analyzed by ELISA. Experimental periodontitis was induced in 9-week-old mice by ligation and oral infection of <em>P. gingivalis</em>, and AITC was injected into the gingiva once daily for 8 days. Alveolar bone resorption was evaluated by measuring the exposed root area. Gene expressions in the periodontal tissue were analyzed via qPCR.</div></div><div><h3>Results</h3><div>AITC exerted weak bacteriostatic effects against <em>P. gingivalis,</em> inhibiting biofilm formation. AITC also impeded the production of interleukin-6 and tumor necrosis factor-α induced by <em>P. gingivalis</em> LPS. Additionally, transient receptor potential ankyrin 1(TRPA1) channel agonist inhibited the anti-inflammatory effects of AITC. In vivo, AITC inhibited alveolar bone destruction and decreased the gene transcription of <em>Il6</em> in the periodontal tissue.</div></div><div><h3>Conclusion</h3><div>AITC exerted weak bacteriostatic and anti-inflammatory effects against <em>P. gingivalis,</em> reducing alveolar bone destruction and suppressing the inflammatory response in experimental periodontitis. Therefore, AITC may serve as a valuable adjunct in controlling periodontal disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106118"},"PeriodicalIF":2.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How malocclusion interferes with tissue inhibitor of metalloproteinase-1 expression and morphology of the articular cartilage of the mandible in female rats","authors":"Carolina Brioschi Mathias , Rebeca Ferreira Badaró , Willian Grassi Bautz , Leticia Nogueira da Gama-de-Souza","doi":"10.1016/j.archoralbio.2024.106117","DOIUrl":"10.1016/j.archoralbio.2024.106117","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this study was to investigate morphological alterations and tissue inhibitor of metalloproteinase-1 expression in the articular cartilage of the mandible under conditions of experimentally induced malocclusion.</div></div><div><h3>Design</h3><div>Twenty-four 8-week-old female Wistar rats were used and divided into control and experimental groups with two different treatment periods (2 and 4 weeks). Sagittal malocclusions were orthodontically created, causing mesial movement of the first molars and distalization of the third molars unilaterally and on opposite sides of the arches. Sagittal sections of the articular cartilage of the mandible were subjected to hematoxylin and eosin and immunohistochemistry for tissue inhibitor of metalloproteinase-1. Chi-square and Mann<img>Whitney U tests were applied.</div></div><div><h3>Results</h3><div>Animals treated for 2 and 4 weeks showed morphological alterations in articular cartilage of the mandible. The main findings were thickening of the posterior third, layer derangement, osteoclast activity and osteophyte formation. Among the cellular aspects, the presence of chondrocytes with condensed nuclei and cytoplasm reduction were observed. The enzyme in control animals was observed only in the mature layer. Treated animals showed immunopositive cells in the proliferative and mature layers, and in the 2-week treated group, the posterior third of the cartilage had more immunolabeled cells than control (<em>P=0.0291</em>).</div></div><div><h3>Conclusions</h3><div>The occlusal disorder caused morphological changes in articular cartilage of the mandible, probably due to the attempt to adapt to the new condition. Tissue inhibitor of metalloproteinase-1 expression may play a role as an initial modulator in the biological events observed in articular cartilage of the mandible.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106117"},"PeriodicalIF":2.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The important role of the Wnt/β-catenin signaling pathway in small molecules mediated gingival mesenchymal stem cells transdifferentiate into neuron-like cells","authors":"Qiuying Liang , Chuhan Zhang , Peiyi Lv , Yongmao Huang , Hang Zhao , Shan Jiang , Wenan Xu","doi":"10.1016/j.archoralbio.2024.106115","DOIUrl":"10.1016/j.archoralbio.2024.106115","url":null,"abstract":"<div><h3>Objective</h3><div>Given their neural crest origin, gingival mesenchymal stem cells (GMSCs) possess high neurogenic potential, which makes them suitable for cell replacement therapy against neurodegenerative diseases. This study investigated whether GMSCs can be transdifferentiated into neurons <em>in vitro</em> using a protocol involving small molecules VCRFY (VPA, CHIR99021, Repsox, Forskolin, and Y-27632). The regulatory mechanisms of key signaling pathways were also investigated.</div></div><div><h3>Methods</h3><div>Neuronal induction of GMSCs was conducted using a small molecules-based protocol over 7 days, which included the evaluation of cell morphology, proliferation, expressions of neurogenic markers, and intracellular calcium oscillation. The activation of canonical the Wnt signaling pathway was assessed by examining the protein content and subcellular localization of β-catenin.</div></div><div><h3>Results</h3><div>Small molecules-treated GMSCs displayed neuronal morphology and increased expression of neurogenic markers, including class III beta-tubulin (TUJ1), neuron-specific enolase (NSE), microtube-associated protein 2 (MAP2), and neurofilament medium (NFM), verified through RT-qPCR, western blotting, and immunocytochemistry. Based on the results of Fluo-4 AM calcium flux assay, small molecules-treated GMSCs exhibited enhanced electrophysiological activity. GMSC proliferation halted after 2 days of treatment. Among the small molecules, CHIR99021 exhibited the highest neuronal induction efficiency. Furthermore, activation of the Wnt/β-catenin signaling pathway augmented neuronal differentiation.</div></div><div><h3>Conclusions</h3><div>Small molecule-based cellular reprogramming can efficiently generate neurons from GMSCs, with Wnt/β-catenin signaling to play a critical role in neuronal induction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106115"},"PeriodicalIF":2.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ali Batuhan Bayırlı , Ceyda Gürhan , Ercan Saruhan
{"title":"Evaluation of salivary melatonin and MMP-9 levels in periodontal diseases","authors":"Ali Batuhan Bayırlı , Ceyda Gürhan , Ercan Saruhan","doi":"10.1016/j.archoralbio.2024.106116","DOIUrl":"10.1016/j.archoralbio.2024.106116","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to evaluate salivary matrix metalloproteinase-9 (MMP-9) and melatonin levels in individuals with periodontal health, gingivitis, and periodontitis.</div></div><div><h3>Design</h3><div>A total of 170 participants were enrolled in this study. They included 57 periodontally healthy individuals, 58 gingivitis patients, and 55 periodontitis patients. Saliva samples were collected by passive drool technique. The levels of MMP-9 and melatonin in saliva were measured biochemically using the ELISA method.</div></div><div><h3>Results</h3><div>Salivary MMP-9 levels in the periodontitis group were significantly higher than those in the gingivitis and periodontally healthy groups, while salivary melatonin levels were significantly lower (<em>p</em><0.001). A positive correlation was observed between clinical periodontal parameters and salivary MMP-9 levels, while salivary melatonin levels were negatively correlated (<em>p</em><0.001). A negative correlation was also observed between salivary MMP-9 levels and salivary melatonin levels (<em>p</em><0.001).</div></div><div><h3>Conclusion</h3><div>This study shows that the level of melatonin in saliva is associated with periodontal disease and with the level of MMP-9 in saliva, which plays a role in this disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106116"},"PeriodicalIF":2.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anne Eriksson Agger , Athina Samara , Tianxiang Geng , Ole Kristoffer Olstad , Janne Elin Reseland
{"title":"Mimicking and in vitro validating chronic inflammation in human gingival fibroblasts","authors":"Anne Eriksson Agger , Athina Samara , Tianxiang Geng , Ole Kristoffer Olstad , Janne Elin Reseland","doi":"10.1016/j.archoralbio.2024.106113","DOIUrl":"10.1016/j.archoralbio.2024.106113","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to identify and validate in vitro conditions that may mimic the translational, cytokine and chemokine profiles observed in human inflamed gingiva in vivo.</div></div><div><h3>Design</h3><div>Primary human gingiva fibroblast cells (HFIB-G) were cultured under serum starvation conditions (0 – 10 %), supplemented with increasing lipopolysaccharide (LPS) concentrations (0.1, 1, or 10 µg/ml) from two bacterial strains <em>E. coli</em> and <em>P. gingivalis</em> and 0.1, 1, or 10 ng/ml recombinant interleukin 1β (IL-1β), alone or in combinations. The levels of cytokines/chemokines were measured in the cell culture medium by Luminex, and gene expression was quantified by Affymetrix microarrays at 24, 48 and 72 h.</div></div><div><h3>Results</h3><div>Inflammation markers were not elevated after stimulation with <em>P. gingivalis</em> LPS, while <em>E. coli</em> LPS and IL-1β individually increased the secretion of interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) to the cell culture medium. IL-1β administration also increased the secretion of several factors, including tumor necrosis factor (TNFα). However, the combination of 1 µg/ml <em>E. coli</em> LPS, 1 ng/ml IL-1β and serum starvation led to increased secretion of IL-6, TNFα, in addition to other factors found in inflamed tissue. Gene expression analyses revealed that this combination not only enhanced the expression interleukins/chemokines genes but also T helper cell signaling and matrix metalloproteinases.</div></div><div><h3>Conclusion</h3><div>Serum reduction in cell culture medium together with the administration of <em>E. coli</em> LPS and IL-1β resulted in gene expression and secreted cytokine/chemokine profiles similar to that found in vivo during chronic inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106113"},"PeriodicalIF":2.2,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Joana Estephany Gordillo Yépez , Renata Machado Marangon , Aline Cristina Batista Rodrigues Johann , Bruno Massa de Viveiros , Patricia Kern Di Scala Andreis , Luana Vosgerau , Sara Moreira Leal Salvação , Orlando Motohiro Tanaka , Odilon Guariza-Filho , Sergio Aparecido Ignácio , Elisa Souza Camargo
{"title":"Impact of botulinum toxin type A on tooth movement and bone remodeling in male Wistar rats","authors":"Joana Estephany Gordillo Yépez , Renata Machado Marangon , Aline Cristina Batista Rodrigues Johann , Bruno Massa de Viveiros , Patricia Kern Di Scala Andreis , Luana Vosgerau , Sara Moreira Leal Salvação , Orlando Motohiro Tanaka , Odilon Guariza-Filho , Sergio Aparecido Ignácio , Elisa Souza Camargo","doi":"10.1016/j.archoralbio.2024.106105","DOIUrl":"10.1016/j.archoralbio.2024.106105","url":null,"abstract":"<div><h3>Objective</h3><div>We evaluated whether the use of botulinum neurotoxin type A (BTX-A) in masticatory muscles influences tooth movement and bone remodeling.</div></div><div><h3>Design</h3><div>Seventy-seven male Wistar rats were allocated to the groups: S - Saline (n=20); SM - Saline with movement (n=20); BT - Botulinum toxin (n=18); BTM - Botulinum toxin with movement (n=19). On day 1, 0.02 mL of sterile 0.9 % saline was administered to groups S and SM and BTX-A (1 U in 0.02 mL of saline) to groups BT and BTM, in the masseter and temporal muscles laterally. On day 30, a nickel titanium spring was installed to move the first maxillary molar and euthanasia was performed on days 32 and 51. Tooth displacement, maxillary and mandibular bone volumes, collagen neoformation, bone and root resorptions, and masseter morphometry were assessed. Statistical analysis was conducted (<em>p</em> < 0.05).</div></div><div><h3>Results</h3><div>A higher percentage of type I collagen was observed in the BT than in the S group on day 51 and lower mass, length, and diameter of the masseter fibers in BT and BTM (<em>p</em> < 0.05). Tooth displacement, bone volume, bone and root resorptions, hyaline area, and masseter height showed no difference among groups with and without BTX-A, regardless of tooth movement (<em>p</em> > 0.05).</div></div><div><h3>Conclusions</h3><div>BTX-A did not interfere with tooth displacement, bone volume, and dental and periodontal tissues related to tooth movement in rats; it increased mature collagen in animals without tooth movement; and it caused a decrease in the mass, length, and diameter of the masseter fibers.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106105"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Didem Sakaryalı Uyar , Hazal Karslıoğlu , Mert Ocak , Hakan Hamdi Çelik
{"title":"Evaluation of hard tissue characteristics and calcifications in pulp tissue of hypomineralized permanent molars using micro-computed tomography","authors":"Didem Sakaryalı Uyar , Hazal Karslıoğlu , Mert Ocak , Hakan Hamdi Çelik","doi":"10.1016/j.archoralbio.2024.106111","DOIUrl":"10.1016/j.archoralbio.2024.106111","url":null,"abstract":"<div><h3>Objectives</h3><div>To determine and compare pulp volume, dentin mineral density, presence of microcracks, pulp stones, and accessory canals, as well as their localizations in root regions for hypomineralized and healthy teeth.</div></div><div><h3>Design</h3><div>This study included 60 extracted permanent molar teeth, categorized into hypomineralized and healthy groups (n = 30 each). The hypomineralized group comprised molar teeth with limited white, yellow, or brown opacities, post-eruptive breakdown, or extensive restoration or crown damage. The healthy group included caries-free molar teeth without these characteristics. Using 3D micro-computed tomography images pulp volume, dentin mineral density, and the presence and locations of microcracks, pulp stones, and accessory canals were determined for each group. Statistical analyses were conducted using Independent T-test and Chi-square test, with significance set at p < 0.05.</div></div><div><h3>Results</h3><div>There was no statistically significant difference between the groups regarding pulp volume and microcracks (p ≥ 0.05). The number of accessory canals was significantly greater in the cervical (p = 0.011; p < 0.05) and middle (p = 0.010; p < 0.05) regions of the hypomineralized teeth than healthy teeth. Dentin mineral density was statistically higher in the apical, middle, and cervical root regions (p < 0.001; p < 0.05); however, the number of pulp stones was found to be greater in the cervical regions of healthy teeth compared with those with hypomineralization (p = 0.026; p < 0.05).</div></div><div><h3>Conclusion</h3><div>There were lower dentin mineral density measurements, a decreased number of pulp stones in the cervical region, and a greater number of accessory canals in the middle and cervical regions of hypomineralized teeth compared with healthy teeth.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106111"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Á. Rubio Salvador , S.A. Jiménez-Brobeil , M. Lozano
{"title":"Analysis of dental chipping for identifying and diagnosing tooth fracture patterns in osteological series","authors":"Á. Rubio Salvador , S.A. Jiménez-Brobeil , M. Lozano","doi":"10.1016/j.archoralbio.2024.106114","DOIUrl":"10.1016/j.archoralbio.2024.106114","url":null,"abstract":"<div><h3>Objective</h3><div>To develop a specific methodology for identifying dental chipping and determining its temporal occurrence in past populations.</div></div><div><h3>Design</h3><div>The analysed sample comprised of 2191 human teeth from various Bronze Age on the Iberian Peninsula (Argar culture, 1900–1450 cal BC). Among these, 471 chipped teeth were identified. Chipping was examined using various microscopic techniques (digital three-dimensional, optical, and confocal), focusing on distribution, morphology, position in the tooth, extent of damage, and post-chipping antemortem modifications (PCAM).</div></div><div><h3>Results</h3><div>The distribution and morphology of the chips enabled the identification chipping mechanism of the chipping, providing valid criteria to distinguish between antemortem and postmortem chipping. Microscopic analyses of the chipping segments—edges, sidewalls, surface, and surrounding area—facilitated determination of the time the chip ocurred (antemortem: recent, less recent, or not recent).</div></div><div><h3>Conclusions</h3><div>While experimental studies provide valuable insights into chipping mechanisms, many criteria may not be applicable to past populations because of the presence of PCAM. The lack of PCAM in some Argaric teeth suggests that previous studies may have underestimated the prevalence of chipping in past populations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106114"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ahmed Elhakim , Ukseong Kim , Euiseong Kim , Sukjoon Lee , Jong-Min Lee , Han-Sung Jung , Sunil Kim
{"title":"Effects of icariin on dental pulp stem cells and its potential applications in dentin repair","authors":"Ahmed Elhakim , Ukseong Kim , Euiseong Kim , Sukjoon Lee , Jong-Min Lee , Han-Sung Jung , Sunil Kim","doi":"10.1016/j.archoralbio.2024.106112","DOIUrl":"10.1016/j.archoralbio.2024.106112","url":null,"abstract":"<div><h3>Objectives</h3><div>As dental pulp therapy evolves towards regenerative approaches, biomolecules such as icariin, derived from <em>Epimedium</em> flowers, are being evaluated for their therapeutic potential. This study investigates icariin's effectiveness in promoting odontogenic differentiation in human dental pulp stem cells (hDPSCs) <em>in vitro</em> and as a pulp-capping agent <em>in vivo</em>.</div></div><div><h3>Design</h3><div>The study explored the effects of icariin on hDPSCs at concentrations of 10, 20, and 40 µM. Cell viability and migration assays were conducted to evaluate cytotoxicity and chemotaxis. Odontogenic differentiation was assessed using alkaline phosphatase staining and alizarin red S (ARS) staining, complemented by real-time PCR and Western blot analyses of key markers such as RUNX family transcription factor 2 (<em>RUNX2</em>), collagen type I alpha 1 chain (<em>COL1A1</em>), alkaline phosphatase (<em>ALPL</em>), and dentin sialophosphoprotein (<em>DSPP</em>). Additionally, the <em>in vivo</em> effects of icariin were tested in a rat maxillary molar model, where icariin-treated collagen sponges were used for direct pulp capping to evaluate its potential to induce reparative dentin formation.</div></div><div><h3>Results</h3><div>Icariin showed no cytotoxic effects on hDPSCs at any tested concentration, enhanced migratory activity in a dose-dependent manner, and significantly increased alkaline phosphatase activity and calcium deposition. Gene and protein expression analyses revealed a dose-dependent increase in odontogenic differentiation markers in icariin-treated hDPSCs. <em>In vivo</em>, icariin effectively promoted reparative dentin formation in exposed rat pulp.</div></div><div><h3>Conclusions</h3><div>Icariin enhances odontogenic differentiation of hDPSCs and has promising potential as a pulp-capping agent for vital pulp therapy.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106112"},"PeriodicalIF":2.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}