Archives of oral biology最新文献

{"title":"Fractionated irradiation induces radioresistant oral carcinoma cells with enhanced malignant phenotypes","authors":"Yangfan Liu ,&nbsp;Tongxu Sun ,&nbsp;Jin Yang,&nbsp;Jingjing Luo,&nbsp;Hongmei Zhou","doi":"10.1016/j.archoralbio.2024.105988","DOIUrl":"10.1016/j.archoralbio.2024.105988","url":null,"abstract":"<div><h3>Objective</h3><p>The fact that certain oral carcinoma patients experience radiotherapy failure implies that a more radioresistant and aggressive phenotype of surviving cancer cells potentially occurs during treatment. Our study aimed to establish radioresistant oral cancer cells through a fractionated irradiation protocol that mimics clinically relevant radiotherapy dosing strategies and to investigate all-round alterations in the malignant phenotype.</p></div><div><h3>Methods</h3><p>Radioresistant oral carcinoma cells were generated by exposing Cal27 and Detroit 562 cells to 60 Gy radiation in 10 dose-escalating fractions and verified by cell immunofluorescence. Specific markers related to the epithelial–mesenchymal transition (EMT) process and the cancer stem cell (CSC) phenotype were assessed by Western blotting. Cell invasion and migration were evaluated using Matrigel-coated transwell and wound healing assays, respectively. Nontargeted metabolomics was used to mechanistically delineate the potential metabolic patterns linked to EMT and CSCs; the CSC phenotype was also examined by sphere formation assays and cell immunofluorescence.</p></div><div><h3>Results</h3><p>Radioresistant oral carcinoma cell lines were successfully established and validated. These cells exhibited enhanced EMT and increase in both cell invasion and migration. These radioresistant cells further demonstrated a high metabolic profile, notably marked by lipid metabolism reprogramming and functional enrichment of ATP-binding cassette (ABC) transporters. Consistently, enhanced CSC phenotype in radioresistant cells was confirmed by elevated expression of stemness markers and increased sphere-forming capacity.</p></div><div><h3>Conclusion</h3><p>Radioresistant oral carcinoma cells subjected to fractionated radiation exhibit an augmented malignant phenotype. The metabolic characteristics linked to enhanced EMT and CSC phenotypes provide potential targets for improving radiotherapy in oral carcinoma.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141038502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supragingival dental biofilm profile and biofilm control during orthodontic treatment with fixed orthodontic appliance: A randomized controlled trial","authors":"T. Zibar Belasic ,&nbsp;M. Badnjevic ,&nbsp;M. Zigante ,&nbsp;B. Mohar Vitezic ,&nbsp;S. Spalj ,&nbsp;E.P. Markova-Car","doi":"10.1016/j.archoralbio.2024.105984","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105984","url":null,"abstract":"<div><h3>Objective</h3><p>The effectiveness of supragingival dental biofilm control during orthodontic treatment and changes in the bacterial profile were analyzed.</p></div><div><h3>Design</h3><p>Sixty-four participants aged 12–22 years (57% female) were included in the study. Participants underwent orthodontic treatment with fixed appliances and were randomly assigned to one of the three groups, which during a period of one month: (I) used chlorhexidine digluconate (CHX), (II) used high concentration of fluoride (F) gel and (III) performed standard oral hygiene. The plaque and gingivitis index, pH of biofilm and white spot lesions (WSL) were assessed. Changes of the bacteria in the biofilm were analyzed by the quantitative polymerase chain reaction</p></div><div><h3>Results</h3><p>Increase in the plaque index, pH of biofilm, and WSL was observed during orthodontic treatment with standard oral hygiene. Large interindividual variability was present, and the effects of one-month use of fluorides and CHX on clinical parameters were not significant. Despite standard hygiene the abundance of studied biofilm bacteria increased - the most <em>Streptoccocus mutans</em> (14.2x) and <em>S. salivarius</em> (3.3x), moderate <em>Veillonella parvula</em> (3x) and the least <em>S. sobrinus</em> (2.3x) and <em>Agregatibacter actinomycetemcomitans</em> (1.9x). The use of CHX reduced <em>S. sobrinus</em> (2.2x) and <em>A. actinomycetemcomitans</em> (1.9x). Fluoride use reduced <em>A. actinomycetemcomitans</em> (1.3x) and <em>S. sobrinus</em> (1.2x). Fluorides better controlled <em>S. mutans</em> than CHX.</p></div><div><h3>Conclusion</h3><p>Bacterial biomass in supragingival biofilm increased during treatment with metal orthodontic appliances, with greater increase in cariogenic bacteria than periopathogens. Fluoride controlled <em>S. mutans</em>, while CHX <em>S. sobrinus</em> and <em>A. actinomycetemcomitans</em>.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140825302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into medieval rural lives: A paleo-odontological investigation of two central European communities","authors":"Antonella Pedergnana ,&nbsp;Roger Seiler ,&nbsp;Renata Huber ,&nbsp;Patrick Eppenberger ,&nbsp;Frank Rühli","doi":"10.1016/j.archoralbio.2024.105985","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105985","url":null,"abstract":"<div><h3>Objective</h3><p>Oral status is an important indicator of past lifestyles. Determining the presence and extent of oral pathologies helps reconstruct average oral health, paramasticatory activities and diet of ancient and historical populations.</p></div><div><h3>Design</h3><p>In this study, the dental remains from the early medieval cemetery of Früebergstrasse in Baar (Canton of Zug, Switzerland) and the high medieval Dalheim cemetery (North Rhine-Westphalia, Germany) were analyzed. Caries, periodontal condition, periapical lesions, antemortem tooth loss, and enamel hypoplasia were assessed in 654 teeth (993 observable loci) from 68 individuals (Baar: n = 36; Dalheim: n = 32).</p></div><div><h3>Results</h3><p>The oral status of both populations was affected by age with higher values of tooth wear in advanced age individuals. High tooth wear values in both populations point towards the consumption of abrasive foods. Pronounced anterior tooth wear in Baar may also be due to non-masticatory tooth usage. Finally, possible nutritional deficiencies were hypothesized for the Baar population. A higher caries prevalence was observed in the Baar group, probably due to differences in carbohydrate intake. The oral conditions observed in the two studied populations exhibited several analogies, suggesting comparable lifestyles despite their separation in space and time. The only differences observed are related to the use of teeth as “tools” and are thus determined by behavioral choices rather than diverse socioeconomic factors.</p></div><div><h3>Conclusions</h3><p>Using multiple dental parameters to examine the oral health of premodern individuals can provide useful insights into the interactions between humans and their environment, from dietary patterns to paramasticatory activities.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001067/pdfft?md5=741a92896b99e7fc5cf324d26f16576d&pid=1-s2.0-S0003996924001067-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140825303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of root shape on canal complexity at the mandibular molar apical surgical resection level: A micro-CT study","authors":"António Ginjeira ,&nbsp;Filipa Neto ,&nbsp;Shirin Behdad ,&nbsp;Inês Farinha ,&nbsp;Nuno Gonçalves ,&nbsp;Jorge N.R. Martins ,&nbsp;Mariana Pires ,&nbsp;Mário Rito Pereira ,&nbsp;Isabel Vasconcelos ,&nbsp;Isabel Duarte","doi":"10.1016/j.archoralbio.2024.105983","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105983","url":null,"abstract":"<div><h3>Objectives</h3><p>This study aimed to investigate the relationship between the aspect ratios of mandibular molar roots at the apical 3-mm level and their root canal complexity.</p></div><div><h3>Design</h3><p>This study used micro-CT imaging to analyze 163 two-rooted mandibular molars. The aspect ratios of the roots at the apical 3-mm level were categorized as \"&lt; 2.75\" or \"≥ 2.75\" (mesial) and \"&lt; 1.75\" or \"≥ 1.75\" (distal). A two-dimensional (2D) analysis focused on four apical axial cross-section levels to determine the presence of main and accessory canals and isthmus. Additionally, a three-dimensional (3D) assessment of the apical 4-mm of both roots examined main and accessory canals, apical foramina, apical deltas, and middle mesial canals.</p></div><div><h3>Results</h3><p>Mesial roots with aspect ratios ≥ 2.75 showed a higher number of main canals at all levels compared to those with aspect ratios &lt; 2.75 at the 3-mm level. Additionally, the ≥ 2.75 group exhibited more accessory canals and a higher average number of accessory canals. The 3D assessment confirmed significantly more accessory canals and apical foramina in the ≥ 2.75 group. The prevalence of roots with apical deltas was nearly double in the ≥ 2.75 group, and middle mesial canals were exclusively found in this group. In the distal root, the ≥ 1.75 group showed a significantly higher number of main canals at all axial levels. No significant differences were observed between groups in terms of accessory canals, apical foramina, or deltas.</p></div><div><h3>Conclusions</h3><p>A higher root aspect ratio is related to higher anatomical complexity.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001043/pdfft?md5=bf3239111df9ffdde1b1f65e61769a56&pid=1-s2.0-S0003996924001043-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140879971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of microorganisms in carious dentin by photodynamic therapy mediated by potassium iodide added to methylene blue and red laser","authors":"Alejandra Brenes-Alvarado ,&nbsp;Felipe Fabrício Farias-da-Silva ,&nbsp;Jorge Rodrigo Soto-Montero ,&nbsp;Juliana Benine-Warlet ,&nbsp;Francisco Carlos Groppo ,&nbsp;Carolina Steiner-Oliveira","doi":"10.1016/j.archoralbio.2024.105978","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105978","url":null,"abstract":"<div><h3>Objective</h3><p>To evaluate the effect of the association of potassium iodide to antimicrobial photodynamic therapy on human carious dentin produced with a microcosm biofilm model.</p></div><div><h3>Methods</h3><p>A microcosm biofilm model was used to generate a caries lesion on human dentin. Pooled human saliva diluted with glycerol was used as an inoculum on specimens immersed on McBain artificial saliva enriched with 1 % sucrose (24 h at 37 °C in 5 % CO₂). After refreshing culture media for 7 days, the dentin specimens were divided in 5 groups (3 specimens per group, in triplicate; n = 9): C (NaCl 0.9 %), CX (2 % chlorhexidine), PKI (0.01 % methylene blue photosensitizer+50 mM KI), L (laser at 15 J, 180 s, 22.7 J/cm²), and PKIL (methylene blue + KI + Laser). After the treatments, dentin was collected, and a 10-fold serial dilution was performed. The number of total microorganisms, total lactobacilli, total streptococci, and <em>Streptococcus mutans</em> was analyzed by microbial counts (CFU/mL). After normality and homoscedasticity analysis, the Welch's ANOVA and Dunnett's tests were used for CFU. All tests used a 5 % significance level.</p></div><div><h3>Results</h3><p>CX and PKIL groups showed significant bacterial decontamination of dentin, compared to group C (p &lt; 0.05) reaching reductions up to 3.8 log₁₀ for CX for all microorganisms’ groups and PKIL showed 0.93, 1.30, 1.45, and 1.22 log₁₀ for total microorganisms, total lactobacilli, total streptococci, and <em>S. mutans</em>, respectively.</p></div><div><h3>Conclusion</h3><p>aPDT mediated by the association of KI and methylene blue with red laser reduced the viability of microorganisms from carious dentin and could be a promising option for cavity decontamination.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140878749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronidase inhibits TGF-β-mediated rat periodontal ligament fibroblast expression of collagen and myofibroblast markers: An in vitro exploration of periodontal tissue remodeling","authors":"Junlin Li ,&nbsp;Chen Chen ,&nbsp;Yunting Zeng ,&nbsp;Jiaqi Lu ,&nbsp;Liwei Xiao","doi":"10.1016/j.archoralbio.2024.105980","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105980","url":null,"abstract":"<div><h3>Objective</h3><p>To determine the effect of hyaluronic acid (HA) degradation by hyaluronidase (HYAL) in inhibiting collagen fiber production by rat periodontal ligament cells (rPDLCs).</p></div><div><h3>Design</h3><p>Primary rPDLCs were isolated from the euthanized rats and used for in vitro experiments. The appropriate HYAL concentration was determined through CCK-8 testing for cytotoxicity detection and Alizarin red staining for mineralization detection. RT-qPCR and western blot assays were conducted to assess the effect of HYAL, with or without TGF-β, on generation of collagen fiber constituents and expression of actin alpha 2, smooth muscle (ACTA2) of rPDLCs.</p></div><div><h3>Results</h3><p>Neither cell proliferation nor mineralization were significantly affected by treatment with 4 U/mL HYAL. HYAL (4 U/mL) alone downregulated type I collagen fiber (<em>Col1a1</em> and <em>Col1a2</em>) and <em>Acta2</em> mRNA expression; however, ACTA2 and COL1 protein levels were only downregulated by HYAL treatment after TGF-β induction.</p></div><div><h3>Conclusions</h3><p>Treatment of rPDLCs with HYAL can inhibit TGF-β-induced collagen matrix formation and myofibroblast transformation.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140815227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of hydroxyapatite nanoparticles coating of titanium surface on biofilm adhesion: An in vitro study","authors":"Gilda Rocha dos Reis-Neta ,&nbsp;Antônio Pedro Ricomini-Filho ,&nbsp;Loyse Martorano-Fernandes ,&nbsp;Vanessa Felipe Vargas-Moreno ,&nbsp;Altair Antoninha Del Bel Cury ,&nbsp;Raissa Micaella Marcello-Machado","doi":"10.1016/j.archoralbio.2024.105986","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105986","url":null,"abstract":"<div><h3>Aim</h3><p>To evaluate the adhesion of mono and duospecies biofilm on a commercially available dental implant surface coated with hydroxyapatite nanoparticles (nanoHA).</p></div><div><h3>Material and methods</h3><p>Titanium discs were divided into two groups: double acid-etched (AE) and AE coated with nanoHA (NanoHA). Surface characteristics evaluated were morphology, topography, and wettability. Mono and duospecies biofilms of <em>Streptococcus sanguinis</em> (<em>S. sanguinis</em>) and <em>Candida albicans</em> (<em>C. albicans</em>) were formed. Discs were exposed to fetal bovine serum (FBS) to form the pellicle. Biofilm was growth in RPMI1640 medium with 10% FBS and 10% BHI medium for 6 h. Microbial viability was evaluated using colony-forming unit and metabolic activity by a colorimetric assay of the tetrazolium salt XTT. Biofilm architecture and organization were evaluated by confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM).</p></div><div><h3>Results</h3><p>AE surface had more pores, while NanoHA had even nanoHA crystals distribution. Roughness was similar (AE: 0.59 ± 0.07 µm, NanoHA: 0.69 ± 0.18 µm), but wettability was different (AE: Θw= 81.79 ± 8.55°, NanoHA: Θw= 53.26 ± 11.86°; P = 0.01). NanoHA had lower <em>S. sanguinis</em> viability in monospecies biofilm (P = 0.007). Metabolic activity was similar among all biofilms. In SEM both surfaces on <em>C. albicans</em> biofilm show a similar distribution of hyphae in mono and duospecies biofilms. AE surface has more <em>S. sanguinis</em> than the NanoHA surface in the duospecies biofilm. CLSM showed a large proportion of live cells in all groups.</p></div><div><h3>Conclusions</h3><p>The nanoHA surface reduced the adhesion of <em>S. sanguinis</em> biofilm but did not alter the adhesion of <em>C. albicans</em> or the biofilm formed by both species.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140894836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of ACAA1 and HADHB as potential prognostic biomarkers based on a novel fatty acid oxidation-related gene model in head and neck squamous cell carcinoma: A retrospective study","authors":"Wan-li Wang ,&nbsp;Hong-jie Jiang ,&nbsp;Ze-liang Shen ,&nbsp;Ya-ling Tang ,&nbsp;Jian Jiang ,&nbsp;Xin-hua Liang","doi":"10.1016/j.archoralbio.2024.105982","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105982","url":null,"abstract":"<div><h3>Objectives</h3><p>To investigate the importance of fatty acid oxidation (FAO)-related genes in predicting the progression and prognosis of head and neck squamous cell carcinoma (HNSCC).</p></div><div><h3>Methods</h3><p>The FAO-related gene prognostic model was established employing Cox regression analyses, during which accuracy and sensitivity of the gene model were evaluated in The Cancer Genome Atlas (TCGA) internal testing and Gene Expression Omnibus (GEO) external validation cohorts. Ultimately, hub genes were identified among 13 model genes using STRING and Cytoscape, with preliminary validation carried out through immunohistochemistry.</p></div><div><h3>Results</h3><p>The model, which comprised 13 genes (ABCD2, ACAA1, ACACB, AKT1, CNR1, CPT1C, CROT, ECHDC2, ETFA, HADHB, IRS2, LONP2, and SLC25A17), was established. On the basis of the median risk score, the two cohorts were grouped into low-and high-risk groups in the subsequent test and validation, and the former exhibited significantly higher survival rates than the latter. Nomograms were established based on prognostic factors, including stage and risk score, and individualized for the prediction of HNSCC patients. Ultimately, immunohistochemical staining showed that ACAA1 and HADHB were significantly under-expressed in HNSCC, with a favorable prognosis associated with low HADHB and high ACAA1.</p></div><div><h3>Conclusions</h3><p>The gene prognostic model has illustrated promising capability in predicting the prognosis, and ACAA1 and HADHB might serve as potential therapeutic biomarkers for HNSCC patients.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140649150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cervical wear pathobiology by robot-simulated 3-year toothbrushing – New methodological approach","authors":"K. Wilke ,&nbsp;S. Nietzsche ,&nbsp;M. Hemmleb ,&nbsp;S. Mason ,&nbsp;R. Varghese ,&nbsp;T. Lang ,&nbsp;P. Gaengler","doi":"10.1016/j.archoralbio.2024.105981","DOIUrl":"https://doi.org/10.1016/j.archoralbio.2024.105981","url":null,"abstract":"<div><h3>Objectives</h3><p>An ex-vivo study was aimed at (i) programming clinically validated robot three-year random toothbrushing, (ii) evaluating cervical macro- and microwear patterns on all tooth groups of different functional age, (iii) documenting and codificating wear related morphological features at the cemento-enamel junction in young teeth and on roots in older teeth.</p></div><div><h3>Design</h3><p>Following ethical approval random toothbrushing (44 strokes per tooth horizontally, rotating, vertically; 2x/d) with manual toothbrushes and low-abrasive dentifrice was performed in an artificial oral cavity with brushing-force 3.5 N on 14 extracted human teeth. Morphological features were examined by SEM at baseline and after simulated 3 years using the replication technique. 3D-SEM analyses were carried out with a four-quadrant back scattered electron detector. Wilcoxon-Mann-Whitney-test was used for statistical analyses.</p></div><div><h3>Results</h3><p>3-year random toothbrushing with horizontal, rotating and vertical brushing movements revealed morphological features classified as four enamel patterns, one dentin pattern and three cervical patterns. Negative impacts were enamel, cementum and dentin loss. Positive impact on oral health was removing dental calculus and straightening cervical traumatic and iatrogenic damages. The volume loss varied from <span><math><mrow><mover><mrow><mi>x</mi></mrow><mo>̅</mo></mover><mo>=</mo><mn>34.25</mn><mi>nl</mi></mrow></math></span> to <span><math><mrow><mover><mrow><mi>x</mi></mrow><mo>̅</mo></mover><mo>=</mo><mn>87.75</mn><mi>nl</mi><mo>.</mo></mrow></math></span> Wear extended apically from 100 to 1500 micrometres.</p></div><div><h3>Conclusion</h3><p>Robot simulated toothbrushing in an artificial oral cavity, with subsequent SEM and 3D-SEM assessment, elucidated both negative and oral health-contributing micromorphology patterns of cervical wear after simulated 3-year random toothbrushing. Cervical macro- and microwear of cementum revealed, for the first time, what we describe as overhanging enamel peninsulas and enamel islands on roots in young teeth, but no enamel islands on roots from older teeth after root cementum loss. In contrast, many older teeth exhibited enamel peninsulas.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Verbascoside inhibits oral squamous cell carcinoma cell proliferation, migration, and invasion by the methyltransferase 3-mediated microRNA-31-5p/homeodomain interacting protein kinase 2 axis","authors":"Yuhua Huang,&nbsp;Wei Wu,&nbsp;Xing Zhang","doi":"10.1016/j.archoralbio.2024.105979","DOIUrl":"10.1016/j.archoralbio.2024.105979","url":null,"abstract":"<div><h3>Objective</h3><p>The study aimed to investigate the effects of verbascoside on oral squamous cell carcinoma (OSCC) cellular behaviors and underlying molecular mechanisms.</p></div><div><h3>Design</h3><p>For this purpose, SCC9 and UM1 cell lines were treated with verbascoside, and their biological behaviors, including proliferation, migration, and invasion, were evaluated using cell counting kit-8, 5-Ethynyl-2′-deoxyuridine, and Transwell assays. The expression of methyltransferase-3 (METTL3), microRNA (miR)− 31–5p, and homeodomain interacting protein kinase-2 (HIPK2) were examined using quantitative real-time polymerase chain reaction (qRT-PCR). The interaction between METTL3 and miR-31–5p was evaluated by RNA immunoprecipitation and methylated RNA immunoprecipitation, while the interaction between miR-31–5p and HIPK2 was evaluated by dual-luciferase reporter analysis.</p></div><div><h3>Results</h3><p>The results indicated inhibition of OSCC cell proliferation, migration, and invasion post verbascoside treatment. Similarly, METTL3 was upregulated in OSCC cells and was inhibited post-verbascoside treatment. Overexpressing METTL3 promoted the cellular processes. Moreover, miR-31–5p was upregulated in OSCC cells, where METTL3 facilitated the processing of miR-31–5p in an N6-methyladenosine (m6A)-dependent manner. The HIPK2 served as miR-31–5p target, where overexpressing miR-31–5p or HIPK2 knockdown reversed the suppression of verbascoside-induced biological behaviors.</p></div><div><h3>Conclusions</h3><p>Verbascoside inhibited the progression of OSCC by inhibiting the METTL3-regulated miR-31–5p/HIPK2 axis. These findings suggested that verbascoside might be an effective drug for OSCC therapy.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":null,"pages":null},"PeriodicalIF":3.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}