Archives of oral biology最新文献

{"title":"Effect of CD10-positive cells on osteogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells","authors":"Tomoaki Sakurai,&nbsp;Masakazu Ishii,&nbsp;Haruka Miyata,&nbsp;Nao Ikeda,&nbsp;Fumio Suehiro,&nbsp;Naohiro Komabashiri,&nbsp;Yurika Oura,&nbsp;Masahiro Nishimura","doi":"10.1016/j.archoralbio.2024.106135","DOIUrl":"10.1016/j.archoralbio.2024.106135","url":null,"abstract":"<div><h3>Objective</h3><div>This study was aimed at investigating the effect of CD10-positive cells within the maxillary/mandibular bone marrow-derived mesenchymal stem cells (MBMSCs) on osteogenic differentiation of MBMSCs.</div></div><div><h3>Design</h3><div>CD10 expression in iliac bone marrow-derived MSCs (IBMSCs), MBMSCs, and gingival fibroblasts was measured using flow cytometry. The osteogenic potential of 19 MBMSC lines was evaluated, and based on it, they were classified into osteogenic-High and osteogenic-Low groups. The percentage of CD10-positive cells in each group was compared. Effect of coculturing gingival fibroblasts and CD10-positive cells on the osteogenic potential of MBMSCs was also assessed. Expression of tissue inhibitor of metalloprotease-1 (TIMP-1) in osteogenic-High and osteogenic-Low MBMSCs was measured using quantitative real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. The molecular mechanisms underlying the regulation of osteogenic differentiation in MBMSCs were investigated.</div></div><div><h3>Results</h3><div>CD10 was not expressed in IBMSCs, but was highly expressed in fibroblasts. In MBMSCs, the CD10-positivity rate varied considerably between cells. MBMSCs with a high-CD10 positivity rate showed low osteogenic potential. Coculture with fibroblasts or CD10-positive cells reduced the osteogenic potential of MBMSCs. TIMP-1 was highly expressed in CD10-positive cells, and osteogenic-Low MBMSCs showed significantly higher TIMP-1 expression compared with osteogenic-High MBMSCs. β-catenin signaling was suppressed in osteogenic-Low MBMSCs.</div></div><div><h3>Conclusion</h3><div>This study revealed that TIMP-1 secreted from CD10-positive cells may be involved in the suppression of the osteogenic potential of MBMSCs by contamination with CD10-positive cells. This finding provides important insights for developing bone regeneration therapies using MBMSCs.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106135"},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary alpha amylase as a diagnostic biomarker for dental caries – A systematic review and meta analysis","authors":"S. Savitha Priyadarsini ,&nbsp;P.G. Naveen Kumar ,&nbsp;Mahesh R. Khairnar ,&nbsp;Zainab Akram ,&nbsp;Ridhi Ghodela ,&nbsp;Sachin Kumar Jadhav ,&nbsp;Neha Shukla","doi":"10.1016/j.archoralbio.2024.106136","DOIUrl":"10.1016/j.archoralbio.2024.106136","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review and meta-analysis aimed to assess the association between the dental caries and the level of salivary alpha amylase activity among caries active and caries free individuals.</div></div><div><h3>Design</h3><div>This review followed the PRISMA guidelines and registered in PROSPERO (CRD42024518973). A comprehensive search across various research databases until January 31, 2024 yielded 1031 articles, where multiple screening ultimately narrowed it to 13 articles. Appropriate cross sectional studies comparing caries active (CA), caries free (CF) group and evaluating their level of salivary alpha amylase activity were included.</div></div><div><h3>Results</h3><div>The meta-analysis was performed on all 13 included studies utilizing a random effects model and two sub group analysis were performed where studies with salivary alpha amylase activity estimated using spectrophotometer and autoanalyzer were analyzed separately among caries active and caries free group. The pooled data reveals that the salivary alpha amylase activity was significantly higher (p = 0.005) in caries active group compared to caries free group with a standardized mean difference of 1.30.</div></div><div><h3>Conclusion</h3><div>This review indicated the differences in the salivary alpha amylase activity among caries active and caries free individuals which could aid in identifying individuals susceptible to dental caries thereby offering new insights for preventive dentistry by assessing their caries risk, enabling tailored preventive strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106136"},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parental transmission effects of the IRF6 polymorphisms among Non-Syndromic Cleft Lip with or without cleft palate in Kerala case parent trios","authors":"Reema Rose Alappat ,&nbsp;Sunish Kadayil Sachith ,&nbsp;Pulikkottil Raphael Varghese ,&nbsp;Puthucode V. Narayanan ,&nbsp;Alex George","doi":"10.1016/j.archoralbio.2024.106134","DOIUrl":"10.1016/j.archoralbio.2024.106134","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this study was to evaluate the <em>IRF6</em> gene polymorphisms in the etiology of the Non-syndromic Orofacial Cleft (NSOFC) population from Kerala.</div></div><div><h3>Design</h3><div>The study included a hundred case-parent triads of NSOFC from the Kerala Population. Genomic DNA was isolated from the trios. The polymorphisms rs2235371 and rs7552506 were genotyped using polymerase chain reaction (PCR) and the Sanger sequencing method. PLINK and haploview software were used for the statistical analysis.</div></div><div><h3>Results</h3><div>We found a substantial association of the rs2235371 (p-value = 0.0094, OR = 2.2) with NSOFC. The allelic TDT analysis ‘A’ allele of rs2235371 showed significant paternal transmission (p-value = 0.03, P_POO=0.506), whereas the ‘C’ allele of rs7552506 (p-value = 0.0094, P_POO=0.0527) showed an excess maternal transmission. The linkage disequilibrium (LD) values (D’= 0.83 and r² =0.033) between rs2235371 and rs7552506 discovered that these two SNPs are not in strong LD. Haplotype A-G exhibited a significantly reduced risk for oral clefts (p=0.035).</div></div><div><h3>Conclusion</h3><div>The current study revealed that polymorphism rs2235371 is associated with NSOFC. The rs2235371 appears to be significant with the trait in the paternal lineage but not when considering both lineages together. On the other hand, rs7552506 shows a significant association in the maternal lineage, with the pooled analysis approaching statistical significance. This study indicates that the <em>IRF6</em> gene may be a risk factor for NSOFC in the Kerala population.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106134"},"PeriodicalIF":2.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between estrogen receptors polymorphisms and temporomandibular joint disorders: A systematic review","authors":"Giusy Rita Maria La Rosa ,&nbsp;Carla Loreto ,&nbsp;Eugenio Pedullà ,&nbsp;Claudia Lombardo","doi":"10.1016/j.archoralbio.2024.106130","DOIUrl":"10.1016/j.archoralbio.2024.106130","url":null,"abstract":"<div><h3>Objectives</h3><div>This systematic review aimed to investigate the association between genetic polymorphisms in estrogen receptor alpha (ERα) and beta (ERβ) and the presence and severity of temporomandibular disorders (TMDs).</div></div><div><h3>Materials and methods</h3><div>A comprehensive search was conducted in PubMed, Scopus and Web of Science on October 19, 2024, without language or time restrictions. Studies were included if they were cross-sectional, case-control, or cohort designs and reported data on ER-polymorphisms and TMDs, diagnosed using validated criteria. Two independent reviewers screened the results to identify eligible studies. Data were extracted and synthesized narratively due to high heterogeneity across included studies, with a subgroup analysis performed to evaluate the influence of sex. The risk of bias was assessed using the Joanna Briggs Institute's critical appraisal tools.</div></div><div><h3>Results</h3><div>The search identified 277 articles, of which 8 studies met the inclusion criteria. Seven were case-control and two cross-sectional studies. The most commonly investigated ER polymorphisms were ESR1 (Pvu II and Xba I) and ESR2 (rs1676303). Some studies identified associations between specific polymorphisms and TMD-related pain or joint conditions, although results varied across different populations and subtypes of TMDs.</div></div><div><h3>Conclusions</h3><div>The findings suggest potential associations between specific ERα and ERβ polymorphisms and TMD risk, particularly in women. However, inconsistencies across studies underscore the need for prospective and larger studies to confirm these genetic links and clarify their clinical significance.</div></div><div><h3>Systematic Review Registration</h3><div>CRD42024581266</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106130"},"PeriodicalIF":2.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic potential of Bauhinia holophylla leaf extracts with conventional antifungals in the inhibition of Candida albicans: A new approach for the treatment of oral candidiasis","authors":"Stéfani de Oliveira Rosa ,&nbsp;Adriana Araújo de Almeida-Apolonio ,&nbsp;João Víctor de Andrade dos Santos ,&nbsp;Cleison da Rocha Leite ,&nbsp;Wellinton Jhon Cupozak-Pinheiro ,&nbsp;Claudia Andréa Lima Cardoso ,&nbsp;Thiago Luis Aguayo de Castro ,&nbsp;Deisiany Gomes Ferreira ,&nbsp;Melyssa Negri ,&nbsp;Kelly Mari Pires de Oliveira","doi":"10.1016/j.archoralbio.2024.106133","DOIUrl":"10.1016/j.archoralbio.2024.106133","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the combination of <em>Bauhinia holophylla</em> (Bong.) Steud. leaf extracts with conventional antifungal agents, highlighting the extracts' potential as adjuvants in treating oral candidiasis.</div></div><div><h3>Design</h3><div>Ethanolic and aqueous extracts of <em>B. holophylla</em> leaves were analyzed using ultra-high-performance liquid chromatography with a diode array detector (UHPLC-DAD) to assess their chemical composition. Their Minimum Inhibitory Concentration (MIC) against standard strains of <em>Candida albicans</em> and isolates from oral mucosa was determined. Additionally, the potential synergistic effects with chlorhexidine gluconate, nystatin, and fluconazole were investigated, along with their impact on inhibiting and disrupting biofilm formation, germ tube formation of <em>C. albicans</em>, and cytotoxicity in human erythrocytes.</div></div><div><h3>Results</h3><div>Protocatechuic acid, epicatechin, and rutin were identified in both extracts. They exhibited fungistatic activity with a median minimum inhibitory concentration (MIC₅₀) of 15.62 µg/mL for the ethanolic extract (EEB) and 62.50 µg/mL for the aqueous extract (AEB) against <em>C. albicans</em>. In growth kinetics, both extracts reduced the viable cell count of <em>C. albicans</em> by 2 logs after 24 h compared to the positive control. The extracts reduced germ tube formation by 81.6 % for EEB and 86.3 % for AEB. The synergistic combination with fluconazole and nystatin resulted in a 50 % reduction in the concentration required to inhibit <em>C. albicans</em> growth. No hemolytic activity was detected in human erythrocytes at the tested concentrations.</div></div><div><h3>Conclusion</h3><div>Both ethanolic and aqueous extracts show promising potential as adjuvants in managing oral candidiasis. Notably, the aqueous extract is advantageous due to its non-toxic solvent, cost-effectiveness, and ease of preparation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106133"},"PeriodicalIF":2.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNA expression profiling in the adult offspring of rats with periodontal disease","authors":"Maria Sara de Lima Coutinho Mattera ,&nbsp;Natália Francisco Scaramele ,&nbsp;Flávia Lombardi Lopes ,&nbsp;Bianca Elvira Belardi ,&nbsp;Thaís Verônica Saori Tsosura ,&nbsp;Heloisa Macedo Sampaio ,&nbsp;Fernando Yamamoto Chiba ,&nbsp;Renato Felipe Pereira ,&nbsp;Rodrigo Martins dos Santos ,&nbsp;Edilson Ervolino ,&nbsp;Gabriele Fernandes Baliero ,&nbsp;Ana Carla Thalez Ywabuchi Nobumoto ,&nbsp;Anna Clara Cachoni ,&nbsp;Antonio Hernandes Chaves-Neto ,&nbsp;Doris Hissako Matsushita","doi":"10.1016/j.archoralbio.2024.106131","DOIUrl":"10.1016/j.archoralbio.2024.106131","url":null,"abstract":"<div><h3>Objective</h3><div>The present study investigated the relationship between maternal periodontal disease, insulin resistance, activation of inflammatory pathways and epigenetic modifications in adult offspring.</div></div><div><h3>Design</h3><div>Therefore, female Wistar rats were divided into control and experimental groups. Seven days after the induction of periodontal disease, female rats from both groups were mated with healthy male rats. After weaning, male offspring were divided into control offspring (CN-o) and periodontal disease offspring (PED-o) groups. Body weight was measured at 0–75 days of age. At day 75, the following were measured in the offspring: insulin resistance by the HOMA-IR index; global miRNAs by microtranscriptome array; validation of the selected miRNAs by quantitative real-time PCR expression; interleukin 1 receptor associated kinase 1 (IRAK1) and tumor necrosis factor receptor-associated factor 6 (TRAF6) content in the gastrocnemius muscle tissue (GSM) by western blotting.</div></div><div><h3>Results</h3><div>Maternal periodontal disease leads to low birth weight (LBW) in the offspring and insulin resistance in adulthood; changes in global miRNA expression (5 miRNAs upregulated and 6 downregulated); and increased protein expression of IRAK1 and TRAF6 in GSM.</div></div><div><h3>Conclusions</h3><div>These findings demonstrate that maternal periodontal disease causes LBW, insulin resistance, activation of inflammatory pathways, and changes in global miRNA expression.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106131"},"PeriodicalIF":2.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"E-cigarette exposure increases caries risk and modifies dental surface in an in vitro model","authors":"Aline Evangelista Souza-Gabriel,&nbsp;Vitoria Leite Paschoini-Costa,&nbsp;Manoel Damião Sousa-Neto,&nbsp;Alice Corrêa Silva-Sousa","doi":"10.1016/j.archoralbio.2024.106128","DOIUrl":"10.1016/j.archoralbio.2024.106128","url":null,"abstract":"<div><h3>Objective</h3><div>Electronic cigarettes have become increasingly popular and can deliver nicotine at levels comparable to traditional tobacco cigarettes. However, the potential adverse effects of these alternative smoking devices on dental health remain uncertain. This study investigates changes in the cariogenic potential of tooth surfaces and analyzes alterations in the chemical composition of aerosols generated from sweet-flavored e-liquids used in electronic cigarettes.</div></div><div><h3>Design</h3><div>Smoking was simulated using an electronic-cigarette testing machine. Eighty specimens of enamel, dentin and root dentin were divided in two groups according smoking: pre-smoke and post-smoke. The response variables were Microhardness (n=10), SEM (n=5) and FTIR (n=5). E-liquid was analyzed pre-smoke and post-smoke by HPLC-UV/Vis. Data were analyzed by one-way ANOVA and Tukey's tests, with a significance level of 5 %. SEM, FTIR and HPLC-UV/Vis data were qualitatively analyzed.</div></div><div><h3>Results</h3><div>Enamel exhibited higher microhardness values before and after smoking, with all substrates showing significant microhardness reduction after smoking. All dentin specimens presented standard dentinal tubules, and post-smoke enamel prisms appeared disorganized with various orientations. The specimens’ chemical constituents remained stable. Dentin and root dentin post-smoke, carbonate band intensity decreased. There was a thermal degradation of e-liquid products and the formation of new compounds post-vaporization.</div></div><div><h3>Conclusions</h3><div>E-cigarette smoking reduces the microhardness of enamel, dentin, and root dentin, alters enamel morphology, induces chemical interactions between e-liquid and tooth tissues, and may increase the risk of cariogenic potential.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106128"},"PeriodicalIF":2.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142696087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histopathological characterization of mandibular condyles in four temporomandibular joint osteoarthritis mouse models","authors":"Ludan Xing ,&nbsp;Xin Qi ,&nbsp;Jiayan Wu ,&nbsp;Yuan Liu ,&nbsp;Liyuan Zhang ,&nbsp;Beizhan Jiang","doi":"10.1016/j.archoralbio.2024.106129","DOIUrl":"10.1016/j.archoralbio.2024.106129","url":null,"abstract":"<div><h3>Objective</h3><div>Temporomandibular joint osteoarthritis (TMJOA) has been modeled in different ways with a lack of uniformity. We aimed to investigate four TMJOA mouse models and assess histopathological changes in condyles, which could assist in the selection of animal models in further TMJOA-related studies.</div></div><div><h3>Design</h3><div>Four TMJOA mouse models were established, including unilateral hyperocclusion, discectomy, monosodium iodoacetate injection and aged model. Bilateral condyles were collected at different time points. The condylar alterations were analyzed by Micro-CT, Hematoxylin and eosin staining, Toluidine blue staining, Safranin O staining, Trap staining, immunofluorescence staining, immunohistochemistry staining and quantitative polymerase chain reaction.</div></div><div><h3>Results</h3><div>Radiographic and histopathological analysis indicated that all four methods could cause condylar degeneration successfully. Differences in morphologic and histologic changes were found among four models. The hyperocclusion model was time-dependent and the lesions got worse over time. Discectomy model presented obvious damage of cartilage and subchondral bone. Injected model showed severe inflammation and chondrocyte hypertrophy. The aged model was characterized by decreased of proteoglycan and osteolysis.</div></div><div><h3>Conclusions</h3><div>The four methods had different characteristics and applicability. The harvest time affected the degree of cartilage degradation. Hyperocclusion was suited to explore the early-stage of TMJOA. Discectomy present advantages in investigating the long-term restoration of cartilage and subchondral bone. Monosodium iodoacetate-injection was appropriate for screening the agents for inflammatory relief. The aged model more naturally facilitated discovering underlying mechanisms in primary TMJOA. Unilateral modeling methods could initiate contralateral condylar alterations. The TMJOA models should be selected based on experimental requirements and applicability of each model.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106129"},"PeriodicalIF":2.2,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142693588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyclosorus terminans extract mitigates submandibular gland changes associated with high-fat diet consumption in male rats","authors":"Nopphakhun Lungruammit ,&nbsp;Hiranya Pintana ,&nbsp;Wasana Pratchayasakul ,&nbsp;Sujinda Songtrai ,&nbsp;Sireewan Kaewsuwan ,&nbsp;Jitjiroj Ittichaichareon ,&nbsp;Nipon Chattipakorn ,&nbsp;Siriporn C. Chattipakorn","doi":"10.1016/j.archoralbio.2024.106127","DOIUrl":"10.1016/j.archoralbio.2024.106127","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate whether the prophylactic effect of <em>Cyclosorus terminans</em> extract mitigates metabolic impairment and submandibular gland changes, as indicated by increased aquaporin5 expression, decreased fibrosis, oxidative stress and inflammation, improved mitochondrial homeostasis/dynamics, and decreased cell death in the submandibular glands of high-fat diet (HFD)-feeding rats.</div></div><div><h3>Methods</h3><div>Thirty-two male Wistar rats were assigned to either a normal diet (ND) as control rats (n=8) or a HFD (n=24) for 12 weeks. The HFD-treated rats were divided into 3 subgroups to receive either: 1) vehicle (HDV), 2) <em>Cyclosorus terminans</em> at a dose of 100 mg/kg/d (HF100), or 3) <em>Cyclosorus terminans</em> at a dose of 200 mg/kg/d (HF200). At week 13, metabolic parameters, systemic oxidative stress, and submandibular gland parameters were assessed.</div></div><div><h3>Results</h3><div>Twelve weeks of HFD-feeding rats induced obese-insulin resistance and submandibular gland changes. Both HF100- and HF200-treated groups improved metabolic parameters and prevented gland changes by reducing fibrosis (TGF-β and p-38), malondialdehyde levels, inflammation (TNF-α, NF-κB, and <em>Ifng</em>), and cell death markers (Caspase 3, GSDMD, and MLKL). Both treatments supported balanced mitochondrial homeostasis/dynamics, as indicated by regulating related genes (<em>Cpt1b, Ndufb8, Mfn1, Mfn2, Opa1,</em> and <em>Dnm1l</em>). However, only the HF200-treated rats restored aquaporin-5 and antioxidants (SOD2 and GPX4) expression to control levels.</div></div><div><h3>Conclusions</h3><div><em>Cyclosorus terminans</em> mitigates metabolic disturbances and submandibular gland changes in HFD-feeding rats. The high dose was more effective, improving gland function by increasing aquaporin5 and antioxidants. These results suggest <em>Cyclosorus terminans</em> may be a promising therapeutic for metabolic disturbances and submandibular gland changes in obese-insulin resistant patients.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106127"},"PeriodicalIF":2.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Measurement of the root surface area in rat molars through three-dimensional modeling","authors":"Yuanyuan Wei ,&nbsp;Danyuan Huang ,&nbsp;Shuo Chen ,&nbsp;Yukun Jiang ,&nbsp;Kuan Yang ,&nbsp;Zhiai Hu ,&nbsp;Hui Li ,&nbsp;Shujuan Zou ,&nbsp;Yuyu Li","doi":"10.1016/j.archoralbio.2024.106132","DOIUrl":"10.1016/j.archoralbio.2024.106132","url":null,"abstract":"<div><h3>Objectives</h3><div>Rats are used as animal models for basic and applied research related to orthodontic tooth movement (OTM). The magnitude of mechanical force in OTM rat models mainly depends on the supporting capability of the periodontal ligament (PDL), which is highly associated with the root surface area (RSA). But the size of rat RSA remains unknown, which is the reason why there are still debates on the magnitude of mechanical force in OTM rat models. This study aimed to explore a method for measuring the RSA in rat molars.</div></div><div><h3>Design</h3><div>The maxillary and mandibular samples of rats were scanned by Micro-CT to generate three-dimensional (3D) images, followed by 3D reconstruction of every molar through Mimics Medical 21.0. Geomagic Wrap 2021 and Unigraphics NX 12.0 were utilized to smooth teeth surface and mark the cementoenamel junction (CEJ). Finally, the RSA in rat molars was measured.</div></div><div><h3>Results</h3><div>The results showed that for the six-, eight-, or ten-week-old rats, the average RSA of maxillary first, second, and third molars was 25.90 ± 2.29 mm², 15.92 ± 2.14 mm², and 10.34 ± 1.94 mm². The RSA of mandibular first, second, and third molars was 27.03 ± 2.63 mm², 17.16 ± 1.61 mm², and 11.39 ± 2.13 mm².</div></div><div><h3>Conclusions</h3><div>Through 3D modelling, we provided data of rat RSA, and observed the trend of increasing RSA mean values with age. These data are pivotal for determining the magnitude of mechanical force required to move rat molars, especially when conducting research related to OTM using rat models.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106132"},"PeriodicalIF":2.2,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}