Archives of oral biology最新文献

{"title":"Biochemical, pathohistological, radiographic and cardiological analysis reveals the possible association between apical periodontitis and cardiac function in diabetic rats","authors":"Khasan Alievich Ordashev ,&nbsp;Andjela Milojevic Samanovic ,&nbsp;Israpil Alisultanovich Omarov ,&nbsp;Vladimir Zivkovic ,&nbsp;Tamara Nikolic Turnic ,&nbsp;Jovana Novakovic ,&nbsp;Aleksandra Stojanovic ,&nbsp;Marina Nikolic ,&nbsp;Bozidar Pindovic ,&nbsp;Jasmina Sretenovic ,&nbsp;Vladimir Petrovich Fisenko ,&nbsp;Sergey Bolevich ,&nbsp;Stefani Bolevich ,&nbsp;Vladimir Jakovljevic","doi":"10.1016/j.archoralbio.2024.106089","DOIUrl":"10.1016/j.archoralbio.2024.106089","url":null,"abstract":"<div><h3>Objective</h3><p>To evaluate the possible effects of apical periodontitis (AP) on cardiac function, structure, and oxidative stress (OS) in rats with diabetes mellitus type 2 (T2DM).</p></div><div><h3>Design</h3><p>Forty-eight (<em>Wistar albino</em>, male) rats were randomized into four groups: control healthy (CTRL), normoglycemic with AP (AP), T2DM, and T2DM with AP (T2DM+AP). T2DM was induced by streptozotocin and a high-fat diet. AP was induced by pulp exposure to the oral environment for 4 weeks and analyzed radiographically. In the blood samples insulin and glucose were established. <em>In vivo,</em> cardiac function was evaluated by echocardiography. <em>Ex vivo</em> cardiac function was assessed by the Langendorff technique. Heart tissue was analyzed pathophysiologically. OS was determined in cardiac tissue homogenate and coronary venous effluent, spectrophotometrically.</p></div><div><h3>Results</h3><p>Impaired glycoregulation was observed in the T2DM+AP group compared to the T2DM, AP, and CTRL groups. The T2DM+AP group was associated with disturbed echocardiography and cardiodynamic parameters. The levels of superoxide anion radical, nitrite, and index of lipid peroxidation were significantly increased, while the superoxide dismutase and catalase were significantly decreased in the T2DM+AP group compared to T2DM, AP, and CTRL groups. The radiographic AP area was significantly larger in the T2DM+AP compared to the AP group.</p></div><div><h3>Conclusion</h3><p>AP was associated with increased glucose levels, impaired cardiac function, structure, and OS in diabetic rats. Diabetes was related to an increased radiographic AP area. The study may be a starting point for further research to clarify the effects of AP on cardiac function in various models of systemic diseases.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106089"},"PeriodicalIF":2.2,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enamel caries lesions, with and without initial cavitation, in relation to anatomical areas of the proximal surface in deciduous molars","authors":"Basílio Rodrigues Vieira ,&nbsp;Leonardo de Freitas Ferreira ,&nbsp;Nathalie Murielly Rolim de Abreu ,&nbsp;Frederico Barbosa de Sousa","doi":"10.1016/j.archoralbio.2024.106081","DOIUrl":"10.1016/j.archoralbio.2024.106081","url":null,"abstract":"<div><h3>Objective</h3><p>proximal enamel caries lesions (PEC) are believed to initiate and progress to cavitation below the proximal contact area (PCA), but no evidence exists on the location of initial carious cavitation on the proximal surface with functional PCA. This study aimed to test the association of anatomical areas of the proximal surface with the severity of PEC and the frequency of cavitation in PEC in primary molars</p></div><div><h3>Design</h3><p>laboratory, observational, transversal study. Exfoliated primary molars (n = 33) with functional PCA (biofilm-free PCA surrounded by biofilm) had their proximal surfaces (one/tooth) divided anatomically into up to nine areas: 3 areas based on the occlusal/cervical PCA boundaries (areas I, II, and III; occluso-cervically) and 3 areas based on the bucco/lingual PCA boundaries (A, B, and C), with area IIB representing the PCA and area IIIB as the sub-PCA (below the PCA). PEC (ICDAS scores 1 and 2–3) and cavitation in PEC were quantified in all areas using stereomicroscopy and microCT. PEC volume was quantified in areas IIB and IIIB under microCT</p></div><div><h3>Results</h3><p>PEC severity increased occluso-cervically. PCA and sub-PCA presented different PEC severities (higher in sub-PCA) and similar PCE volumes, but the odds of carious cavitation were much higher (Odds ratio = 197.4; 95 % CI: 8.7/4480.7) in the PCA than in the sub-PCA (no cavitation).</p></div><div><h3>Conclusion</h3><p>PCA presented lower PEC severity and similar PEC volume compared to sub-PCA, but PCA concentrated all cavitations in PEC, supporting a new model for the pathogenesis of PEC.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106081"},"PeriodicalIF":2.2,"publicationDate":"2024-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142142029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spatio-temporal expression of Sox2+ progenitor cells regulates the regeneration of rat submandibular gland","authors":"Dan Li ,&nbsp;Yi Qu ,&nbsp;Bin Wang ,&nbsp;Haoyang Zhang ,&nbsp;Lizheng Qin","doi":"10.1016/j.archoralbio.2024.106080","DOIUrl":"10.1016/j.archoralbio.2024.106080","url":null,"abstract":"<div><h3>Objective</h3><p>Sox2 plays crucial roles in tissues homeostasis and regeneration. However, there are lack of a comprehensive examination of Sox2 expression and its functional role in submandibular gland regeneration. Therefore, we aimed to elucidate the impact of Sox2 on submandibular gland regeneration.</p></div><div><h3>Materials and Methods</h3><p>A Sprague-Dawley rat submandibular gland duct ligation/de-ligation regeneration model was conducted in this study. Sox2-shRNA vectors were retro-ductally administered into the submandibular gland to establish a stable Sox2 knockdown model. Conventional histopathological and molecular biological methods were used to investigate phenotypic changes.</p></div><div><h3>Results</h3><p>The submandibular gland normalized completely 28 days after ligature removal (following 7 days of duct ligation). AQP5 expression gradually increased after ligation removal until returning to normal levels. In submandibular gland regeneration, Sox2 re-expressed and co-expressed with AQP5⁺ acinar cells, and Sox2 expression peaked on day 14, recovered to normal on day 28, reproducing the developmental pattern. Sox2 knockdown hindered gland regeneration and induced irreversible fibrosis. The AQP5 expression was significantly lower than the contemporaneous solely ligated group, while the blue collagen deposition and the Vimentin expression increased prominently. The expression of CD68, IL-1β, TNF-α and IL-17A increased significantly, and epithelial cells in the Sox2 knockdown group expressed higher levels of IL-17A.</p></div><div><h3>Conclusions</h3><p>These findings highlight Sox2 as a crucial regulator of the acinar cell lineage. Sox2⁺ progenitor cells are pivotal for acinar cell maintenance, which is indispensable for submandibular gland regeneration. Collectively, our findings may help develop targeted interventions for enhancing tissue repair and preventing irreversible fibrosis in salivary gland disorders.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106080"},"PeriodicalIF":2.2,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142096646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiota: Taxonomic composition and functional profile in caries-free and in caries-affected individuals – A systematic review","authors":"Maria Eduarda Lisbôa Pagnussatti ,&nbsp;Heitor Sales de Barros Santos ,&nbsp;Clarissa Cavalcanti Fatturi Parolo ,&nbsp;Juliana Balbinot Hilgert ,&nbsp;Rodrigo Alex Arthur","doi":"10.1016/j.archoralbio.2024.106070","DOIUrl":"10.1016/j.archoralbio.2024.106070","url":null,"abstract":"<div><h3>Objective</h3><p>To compare the oral microbiota among caries-free (CF) with caries-affected (CA) individuals, both at taxonomic and at functional levels.</p></div><div><h3>Design</h3><p>This systematic review was conducted following PRISMA guidelines. A structured search was carried out in MEDLINE/PUBMED, Web of Science, EMBASE, LILACS, SciELO, Scopus and Google Scholar databases up to September, 2023. Observational studies, without any restriction on date of publication and using next-generation targeted or untargeted sequencing methods for identification of microbial communities were included. Qualitative synthesis was performed from all included studies.</p></div><div><h3>Results</h3><p>54 studies were included (43 cross-sectional; 11 cohort) comprising more than 3486 participants (at least 1666 CF and 1820 CA) whose saliva and/or dental plaque were used as clinical samples. Methodological quality was graded as “fair” for most of the studies. The abundance of 87 bacterial and 44 fungal genera were statistically different among CF and CA individuals. <em>Atopobium</em> spp., <em>Capnocytophaga</em> spp., <em>Lactobacillus</em> spp., <em>Prevotella</em> spp., <em>Scardovia</em> spp., <em>Selenomonas</em> spp. among others were frequently reported as being more abundant in CA individuals. Several functional patterns, such as lipids, carbohydrate, starch, sucrose, amino sugar metabolisms, among others, were identified as being specifically related to CF or to CA conditions.</p></div><div><h3>Conclusion</h3><p>In spite of the variability among the included studies and of the predominance of qualitative synthesis, groups of microorganisms as well as specific functional profiles coded by the assessed microbiota are differently abundant among caries-affected and caries-free individuals. These results need to be interpreted with caution considering the limitations inherent to each assessed primary study.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106070"},"PeriodicalIF":2.2,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142122804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CCKR signaling map, G-Protein bindings, hormonal regulation, and neural mechanisms may influence the osteogenic/cementogenic differentiation potential of hPDLSCs","authors":"Catharina Marques Sacramento ,&nbsp;Miki Taketomi Saito ,&nbsp;Márcio Zaffalon Casati ,&nbsp;Enilson Antonio Sallum ,&nbsp;Renato Correa Viana Casarin ,&nbsp;Karina Gonzales Silvério","doi":"10.1016/j.archoralbio.2024.106069","DOIUrl":"10.1016/j.archoralbio.2024.106069","url":null,"abstract":"<div><h3>Objective</h3><p>Periodontal regeneration poses challenges due to the periodontium's complexity, relying on mesenchymal cells from the periodontal ligament (hPDLSCs) to regenerate hard tissues like bone and cementum. While some hPDLSCs have high regeneration potential (HOP-hPDLSCs), most are low potential (LOP-hPDLSCs). This study analyzed hPDLSCs from a single donor to minimize inter-individual variability and focus on key differences in differentiation potentials.</p></div><div><h3>Design</h3><p>This study used RNA-seq, genomic databases, and bioinformatics tools to explore signaling pathways (SPs), biological processes (BPs), and molecular functions (MFs) guiding HOP cells to mineralized matrix production. It also investigated limitations of LOP cells and strategies for enhancing their osteo/cementogenesis.</p></div><div><h3>Results</h3><p>In basal conditions, HOP exhibited a multifunctional gene network with higher expression of genes related to osteo/cementogenesis, cell differentiation, immune modulation, stress response, and hormonal regulation. In contrast, LOP focused on steroid hormone biosynthesis and nucleic acid maintenance. During osteo/cementogenic induction, HOP showed strong modulation of genes related to angiogenesis, cell division, mesenchymal differentiation, and extracellular matrix production. LOP demonstrated neural synaptic-related processes and preserved cellular cytoskeleton integrity. CCKR map signaling and G-protein receptor bindings gained significance during osteo/cementogenesis in HOP-hPDLSCs. Both HOP and LOP shared common BPs related to gastrointestinal and reproductive system development.</p></div><div><h3>Conclusion</h3><p>The osteo/cementogenic differentiation of HOP cells may be regulated by CCKR signaling, G-protein bindings, and specific hormonal regulation. LOP cells seem committed to neural mechanisms. This study sheds light on hPDLSCs' complex characteristics, offering a deeper understanding of their differentiation potential for future periodontal regeneration research and therapies.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106069"},"PeriodicalIF":2.2,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142089192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"PRELP inhibits the progression of oral squamous cell carcinoma via inactivation of the NF-κB pathway","authors":"Xiaoni Sun,&nbsp;Luyi Chai,&nbsp;Bingjie Wang,&nbsp;Jianbo Zhou","doi":"10.1016/j.archoralbio.2024.106068","DOIUrl":"10.1016/j.archoralbio.2024.106068","url":null,"abstract":"<div><h3>Objectives</h3><p>The aim of this study was to investigate the role and molecular mechanism of proline/arginine-rich end leucine-rich repeat protein (PRELP), a secreted protein in extracellular matrix, in oral squamous cell carcinoma (OSCC) progression.</p></div><div><h3>Design</h3><p>PRELP expression in OSCC was analyzed in the Gene Set Enrichment (GSE) 138206, GSE37991, and GSE23558 datasets as well as cell lines. Also, PRELP expression and its relationship with prognosis and immune infiltration in head and neck squamous cell carcinoma (HNSCC) were confirmed by bioinformatics analysis. The proliferation, apoptosis, invasion, epithelial-to-mesenchymal transition (EMT) and NF-κB activation were detected after alteration of PRELP expression in OSCC cells using CCK-8, EdU, flow cytometry, Transwell, real-time PCR, immunofluorescence and Western blot. Additionally, an NF-κB inhibitor PDTC was used to confirm the regulation mechanism of PRELP.</p></div><div><h3>Results</h3><p>The expression of PRELP in OSCC tissues, cells and in HNSCC samples was low. HNSCC patients with higher PRELP expression was associated with longer overall survival. A positive correlation between PRELP expression and immune cell infiltration was found in HNSCC. Upregulation of PRELP inhibited, whereas PRELP silencing promoted, the proliferation, invasion and EMT of OSCC cells. Also, overexpression of PRELP promoted cell apoptosis. Mechanistically, PRELP suppressed p65 phosphorylation and nuclear translocation. And PDTC treatment partially reversed the influences of PRELP knockdown on the malignant behaviors in OSCC cells.</p></div><div><h3>Conclusion</h3><p>PRELP suppressed OSCC progression via inactivation of the NF-κB pathway. Targeting PRELP may be a potential approach for OSCC treatment.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106068"},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141991253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oral microbiome insights: Tracing acidic culprits in dental caries with functional metagenomics","authors":"Annapurna Gupta ,&nbsp;A. Shivachandran ,&nbsp;Lilly M. Saleena","doi":"10.1016/j.archoralbio.2024.106064","DOIUrl":"10.1016/j.archoralbio.2024.106064","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to investigate the presence and abundance of acid-producing bacteria in dental caries samples using functional gene prediction techniques.</p></div><div><h3>Design</h3><p>A total of 24 dental caries samples were collected for analysis. DNA isolation was performed followed by shotgun metagenomic sequencing. Functional gene prediction techniques were used to identify enzymes responsible for acid production from primary metabolites. Enzymes responsible for converting primary metabolites into acids were identified from the KEGG database. Subsequently, 840 contigs were examined, and their genus and species were characterized.</p></div><div><h3>Results</h3><p>Analysis of the obtained data revealed 31 KEGG IDs corresponding to enzymes involved in the conversion of primary metabolites into acids. All 117 identified genera from the contig analysis were found to be part of the oral microbiome. In addition, A higher prevalence of acid-producing bacteria was noted in dental caries samples compared to earlier reports.</p></div><div><h3>Conclusion</h3><p>The study indicates the significant role of acid-producing bacteria in the initiation and progression of dental caries. The findings highlight the importance of microbial activity in the demineralization process of tooth enamel. Methods for preventing dental decay may be promising if specific measures are implemented to reduce the amount of acid produced by oral bacteria.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106064"},"PeriodicalIF":2.2,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142096645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Testing the patterning cascade model of cusp development in Macaca fascicularis mandibular molars","authors":"Simon A. Chapple ,&nbsp;Tanya M. Smith ,&nbsp;Matthew M. Skinner","doi":"10.1016/j.archoralbio.2024.106067","DOIUrl":"10.1016/j.archoralbio.2024.106067","url":null,"abstract":"<div><h3>Objective</h3><p>Molar crown configuration plays an important role in systematics, and functional and comparative morphology. In particular, the number of cusps on primate molars is often used to identify fossil species and infer their phylogenetic relationships. However, this variability deserves renewed consideration as a number of studies now highlight important developmental mechanisms that may be responsible for the presence of molar cusps in some mammalian taxa. Experimental studies of rodent molars suggest that cusps form under a morphodynamic, patterning cascade model of development (PCM) that involve the iterative formation of enamel knots. This model posits that the size, shape and location of the first-forming cusps determines the presence and positioning of later-forming cusps.</p></div><div><h3>Design</h3><p>Here we test whether variation in accessory cusp presence in 13 <em>Macaca fascicularis</em> mandibular second molars (M2s) is consistent with predictions of the PCM. Using micro-CT, we imaged these M2s and employed geometric morphometrics to examine whether shape variation in the enamel-dentine junction (EDJ) correlates with accessory cusp presence.</p></div><div><h3>Results</h3><p>We find that accessory cusp patterning in macaque M2s is broadly consistent with the PCM. Molars with accessory cusps were larger in size and possessed shorter relative cusp heights compared to molars without accessory cusps. Peripheral cusp formation was also associated with more centrally positioned primary cusps, as predicted by the PCM.</p></div><div><h3>Conclusions</h3><p>While these results demonstrate that a patterning cascade model is broadly appropriate for interpreting cusp variation in <em>Macaca fascicularis</em> molars<em>,</em> it does not explain all manifestations of accessory cusp expression in this sample.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106067"},"PeriodicalIF":2.2,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0003996924001882/pdfft?md5=a0e62a0516c8fac801db9be99e5b8f65&pid=1-s2.0-S0003996924001882-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141984670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The influence of simvastatin on osteoblast functionality in the presence of titanium dioxide particles In-vitro","authors":"Ahmad Fawaz ,&nbsp;Marwan Mansoor Mohammed ,&nbsp;Asmaa Ismail ,&nbsp;K.G.Aghila Rani ,&nbsp;A.R. Samsudin","doi":"10.1016/j.archoralbio.2024.106065","DOIUrl":"10.1016/j.archoralbio.2024.106065","url":null,"abstract":"<div><h3>Objective</h3><p>Leaching of particles from dental titanium implant surfaces into preimplant microenvironment causes detrimental effects on bone cells. The current study investigated influence of simvastatin in mitigating adverse pro-inflammatory effects of titanium dioxide (TiO₂) micro (MP) and nano (NP) particles on hFOB 1.19 cells <em>in vitro.</em></p></div><div><h3>Design</h3><p>Viability of hFOB 1.19 cells following exposure to varying concentrations of TiO₂ MPs and NPs and simvastatin were measured by XTT assay. hFOB 1.19 cells were treated with 100 µg/mL of TiO₂ MPs, 100 µg/mL of TiO₂ NPs, 0.1 µM simvastatin, 100 µg/mL of TiO₂ MPs+ 0.1 µM simvastatin and 100 µg/mL of TiO₂ NPs+ 0.1 µM simvastatin. After 24 h, ROS was measured by flow cytometry. On day 14, real-time PCR analysis for pro-inflammatory cytokines and bone formation markers was done for TNFα, IL1β, osteocalcin, ALP, and Col1 markers; while ALP and RANKL/OPG ratio were determined by colorimetric and ELISA assays respectively. Further, mineralization study using Alizarin Red S staining (ARS) and calcium quantification were performed.</p></div><div><h3>Results</h3><p>Exposure of hFOB to TiO₂ MPs and NPs generated ROS and reduced cell viability significantly, with upregulation of pro-inflammatory markers TNFα and IL1β and downregulation of bone formation markers OC and increased RANKL/OPG ratio and lowered degree of mineralization. Treatment with 0.1 µM of simvastatin treatment reversed the effects by mitigating oxidative stress, dampening pro-inflammatory markers, upregulation of bone formation markers, lowering RANKL/OPG ratio and increasing degree of mineralization.</p></div><div><h3>Conclusion</h3><p>Simvastatin possesses antioxidant, anti-inflammatory, and pro-osteogenic properties that may support bone healing around titanium implants.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"167 ","pages":"Article 106065"},"PeriodicalIF":2.2,"publicationDate":"2024-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141985780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnostic significance of LncRNA MIAT in periodontitis and the molecular mechanisms influencing periodontal ligament fibroblasts via the miR-204-5p/DKK1 axis","authors":"Yu Ren ,&nbsp;Jiwen Zheng ,&nbsp;Yang Cao ,&nbsp;Yu Zhu ,&nbsp;Zhuo Ling ,&nbsp;Zhiqiang Zhang ,&nbsp;Mingke Huang","doi":"10.1016/j.archoralbio.2024.106066","DOIUrl":"10.1016/j.archoralbio.2024.106066","url":null,"abstract":"<div><h3>Objective</h3><p>This study investigated the clinical importance of long noncoding RNA myocardial infarction-associated transcript (MIAT) in periodontitis and its impact on the functional regulation of human periodontal ligament fibroblasts (hPDLFs).</p></div><div><h3>Methods</h3><p>Ninety-eight periodontitis patients and 74 healthy controls were enrolled. In vitro cellular models were created using <em>Porphyromonas gingivalis</em> lipopolysaccharide (Pg-LPS) to stimulate hPDLFs. Real-time quantitative polymerase chain reaction was used to measure mRNA levels of MIAT and osteogenic factors. Inflammation factor concentration was assessed using an enzyme-linked immunosorbent assay. Cell viability and apoptosis were examined by cell counting kit −8 and flow cytometry assay. The targeting relationship was verified by the dual-luciferase reporter and RNA Immunoprecipitation assay.</p></div><div><h3>Results</h3><p>Highly expressed MIAT and Dicckopf-1 (DDK1), and lowly expressed miR-204–5p were found in the gingival crevicular fluid of periodontitis patients and Pg-LPS induced hPDLFs. MIAT has a sensitivity of 76.53 % and a specificity of 86.49 % for identifying patients with periodontitis among healthy individuals. MIAT acts as a sponge for miR-204–5p and upregulates DDK1 mRNA expression. Silencing of MIAT diminished the promotion of apoptosis and inflammation in hPDLFs by Pg-LPS and enhanced osteogenic differentiation. However, a miR-204–5p inhibitor significantly reversed the effect of silenced MIAT.</p></div><div><h3>Conclusions</h3><p>MIAT may act as a promising biomarker for periodontitis. It modulates apoptosis, inflammation, and osteogenic differentiation of PDLFs by focusing on the miR-204–5p/DKK1 axis, indicating its potential as a new therapeutic target for treating periodontitis.</p></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"168 ","pages":"Article 106066"},"PeriodicalIF":2.2,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142076731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}