Archives of oral biology最新文献

{"title":"Profiling mRNA encoding glucocorticoid receptor α in saliva: Relationship to hair cortisol levels in individuals aged 15–25 years","authors":"Naima Abouseta ,&nbsp;Noha Gomaa ,&nbsp;Ali Tassi ,&nbsp;S. Jeffrey Dixon ,&nbsp;Krishna Singh ,&nbsp;Sharat C. Pani","doi":"10.1016/j.archoralbio.2024.106158","DOIUrl":"10.1016/j.archoralbio.2024.106158","url":null,"abstract":"<div><h3>Objective</h3><div>We assessed levels of mRNA encoding two glucocorticoid receptor (GR) isoforms (GRα and GRβ) in saliva and examined their relationship with hair cortisol levels and dental caries experience.</div></div><div><h3>Design</h3><div>Adolescents and young adults were assessed for dental caries experience, and hair cortisol was measured by ELISA. RNA was extracted from whole saliva using TRIzol, followed by quantitative real-time PCR analysis of GRα, GRβ, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH).</div></div><div><h3>Results</h3><div>GRβ mRNA was not detectable in most samples, whereas GRα mRNA was observed in all samples. There were significantly lower levels of GRα mRNA in individuals with elevated hair cortisol levels than in those with normal cortisol levels. Levels of GRα mRNA did not differ significantly in individuals with dental caries experience compared to individuals with no caries experience.</div></div><div><h3>Conclusions</h3><div>We identified and quantified mRNA encoding GRα in saliva. Its levels were inversely associated with hair cortisol (a marker of chronic stress). Although caries experience was associated with hair cortisol levels, there was no significant association between GRα levels and caries experience. Chronic stress has been proposed to be associated with reduced expression of GRα and this association appears to hold for GRα mRNA levels in saliva.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106158"},"PeriodicalIF":2.2,"publicationDate":"2024-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy of combining soluble calcium and pyrophosphate with sodium fluoride in inhibiting enamel caries progression: An in vitro study","authors":"Masashi Fujiki,&nbsp;Chika Akabane,&nbsp;Mitsuo Kimura,&nbsp;Kei Kurita","doi":"10.1016/j.archoralbio.2024.106156","DOIUrl":"10.1016/j.archoralbio.2024.106156","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to compare the effects of a combination of sodium fluoride, soluble calcium, and pyrophosphate (FCaP) versus fluoride alone in inhibiting enamel caries progression.</div></div><div><h3>Design</h3><div>Different FCaP solutions were prepared, and two were selected for testing (FCaP-1: F = 76 mmol/L, Ca = 7.6 mmol/L, P = 7.6 mmol/L, FCaP-5: F = 76 mmol/L, Ca = 23 mmol/L, P = 23 mmol/L). Fluoride solution (F = 76 mmol/L) was used as a control. Fluoride and calcium bioavailability in the solutions were measured, and NMR analysis was used to identify fluorine-containing complexes. Sound bovine enamel samples (n = 24 / group) underwent a 4-day pH cycling protocol followed by an additional 3 days of demineralization. Micro-hardness testing and fluoride concentration measurements were performed.</div></div><div><h3>Results</h3><div>FCaP-1 and FCaP-5 demonstrated nearly 100 % fluoride and calcium bioavailability. NMR analysis confirmed the formation of fluorine-containing complex. Enamel treated with FCaP-5 exhibited significantly less reduction in subsurface hardness after pH cycling and additional demineralization compared to fluoride alone. Interestingly, fluoride concentration and acid resistance on enamel surfaces treated with FCaP-5 was lower than with fluoride alone (Steel’s multiple comparison test, p &lt; 0.05).</div></div><div><h3>Conclusions</h3><div>FCaP effectively inhibits caries progression in subsurface enamel layers under pH cycling conditions by providing bioavailable calcium, indicating that FCaP increases the effectiveness of fluoride in caries management. FCaP may be a valuable addition to clinical practice, particularly for improving the effectiveness of fluoride-containing oral care products in individuals with low salivary calcium levels.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106156"},"PeriodicalIF":2.2,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Are hypoxia-related proteins associated with the invasiveness of glandular odontogenic cysts? A multicenter study","authors":"Rafaela de Albuquerque Dias ,&nbsp;Karolyny Martins Balbinot ,&nbsp;Karine Duarte da Silva ,&nbsp;Ana Paula Neutzling Gomes ,&nbsp;Carla Mosconi ,&nbsp;Elismauro Franscisco de Mendonça ,&nbsp;Sandra Beatriz Chaves Tarquinio ,&nbsp;Sérgio de Melo Alves Junior ,&nbsp;Maria Cássia Ferreira de Aguiar ,&nbsp;João de Jesus Viana Pinheiro","doi":"10.1016/j.archoralbio.2024.106151","DOIUrl":"10.1016/j.archoralbio.2024.106151","url":null,"abstract":"<div><h3>Objective</h3><div>The study aimed to investigate the expression of hypoxia markers associated with invadopodia in glandular odontogenic cysts and to explore an association between this expression with the aggressive biological behaviour of this odontogenic cyst.</div></div><div><h3>Design</h3><div>Immunohistochemistry was employed to assess the expression of hypoxia-inducible factor 1 alpha (HIF-1α), notch homologous protein of the neurogenic locus 1 (NOTCH-1), disintegrin and metalloproteinase-12 (ADAM-12), and heparin-binding epidermal growth factor (HB-EGF) in 17 samples of glandular odontogenic cysts, 10 samples of calcifying odontogenic cysts, and 10 samples of dental follicles.</div></div><div><h3>Results</h3><div>The glandular odontogenic cyst samples exhibited increased expression of HIF-1α, NOTCH-1, ADAM-12 and HBEGF proteins compared with calcifying odontogenic cyst and dental follicle samples. HIF-1α demonstrated localization primarily within the nuclei of cystic epithelial cells of the glandular odontogenic cyst. NOTCH-1 and ADAM-12 exhibited expression in the cytoplasm and nuclei of epithelial and mucous cells of the glandular odontogenic cyst, of whereas HB-EGF was predominantly expressed in the cytoplasm. Weak labeling of these proteins was observed in the odontogenic epithelium of the calcifying odontogenic cyst and dental follicle samples.</div></div><div><h3>Conclusions</h3><div>The hypoxia-related signaling proteins are overexpressed in glandular odontogenic cyst when compared with calcifying odontogenic cyst and dental follicle. The reported aggressiveness of glandular odontogenic cyst can be partially explained by the expression of these proteins.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106151"},"PeriodicalIF":2.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142792938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The activation level of the floor-of-the-mouth muscles is systematically modulated using tongue-pressing and swallowing tasks in healthy elderly","authors":"Jong-Chi Oh","doi":"10.1016/j.archoralbio.2024.106155","DOIUrl":"10.1016/j.archoralbio.2024.106155","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to examine how muscle activity of the floor-of-the-mouth (FOM) muscles changes with different target exercise intensities of 40 %, 60 %, 80 %, and 100 % of maximum isometric pressure (MIP) during tongue-pressing and swallowing tasks in healthy elderly.</div></div><div><h3>Design</h3><div>This prospective, repeated-measures within-participant study included 35 participants (mean age: 75.2 ± 4.8 years, 26 women). Each participant performed 16 tasks using the Iowa Oral Performance Instrument (IOPI) in a randomized order, with two repetitions per task (anterior/posterior tongue × pressing/swallowing task × 40/60/80/100 % of the MIP). Furthermore, FOM-muscle activity during the task was simultaneously measured using surface electromyography. Statistical analysis was conducted using repeated-measures analysis of variance (ANOVA).</div></div><div><h3>Results</h3><div>Significant differences in FOM-muscle activity were observed across most intensity levels during pressing tasks (<em>p</em> &lt; 0.001), with fewer significant differences noted during swallowing tasks, particularly involving the posterior tongue. A significant correlation was found between tongue pressure of the anterior/posterior tongue and FOM-muscle activation level (<em>p</em> &lt; 0.001 and <em>r</em> = 0.517 and 0.447 fo<em>r</em> the isometric tongue pressure of the anterior and posterior tongue, respectively; <em>r</em> = 0.370 for the swallowing pressure of the anterior tongue) except for posterior tongue swallowing task.</div></div><div><h3>Conclusions</h3><div>These findings suggest that healthy elderly can systematically modulate suprahyoid muscle activity using a tongue-pressure-measurement device when performing accuracy training. However, they may require personalized systematic feedback and sufficient practice. The study underscores the potential of the IOPI in swallowing rehabilitation for elderly, while also highlighting the need for further research on patients with dysphagia.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106155"},"PeriodicalIF":2.2,"publicationDate":"2024-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142795875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tensile stress induced osteogenesis of periodontal ligament cells via Piezo1 mediated TAZ-Cbfα1 signaling","authors":"Sisi Li ,&nbsp;Ting Jin ,&nbsp;Yi Wang ,&nbsp;Hui Deng ,&nbsp;Rongdang Hu","doi":"10.1016/j.archoralbio.2024.106152","DOIUrl":"10.1016/j.archoralbio.2024.106152","url":null,"abstract":"<div><h3>Objective</h3><div>Cyclic tensile stress (CTS) is known to induce osteogenesis of periodontal ligament cells (PDLCs), in which the molecular mechanism remains to be elucidated. This study aimed to investigate the role of the mechanosensitive calcium channel Piezo1 in the osteogenesis of PDLCs under tensile strain, as well as the signal regulation of the TAZ-Cbfα1 pathway in this process.</div></div><div><h3>Design</h3><div>PDLCs were isolated from periodontal ligament tissues and subjected to CTS. Alizarin red staining (ARS) and alkaline phosphatase (ALP) assay were used to detect the osteogenesis of PDLCs. RT-qPCR and Western blot were used to detect the transcripts and protein expression levels of Piezo1, Transcriptional co-activator with PDZ binding motif (TAZ), and Core-binding factor α1 (Cbfα1) respectively. Immunofluorescence staining was used to detect the nuclear aggregation of TAZ. Small interfering RNA (siRNA) targeting Piezo1 (Piezo1-siRNA) was adopted to inhibit Piezo1 mRNA expression.</div></div><div><h3>Results</h3><div>The results showed that the osteogenic differentiation capacity of PDLCs was significantly enhanced under CTS, along with elevated mRNA and protein expression levels of Piezo1, TAZ, and Cbfα1. Moreover, the ALP activity and the formation of calcium nodules by ARS staining were significantly increased. In addition, Piezo1 siRNA infection significantly inhibited the CTS-induced TAZ-Cbfα1 pathway and the osteogenesis of PDLCs, suggesting the regulatory role of Piezo1.</div></div><div><h3>Conclusions</h3><div>We provided evidence that the application of CTS encourages the osteogenic differentiation of PDLCs, which could be mediated by the Piezo1 targeted TAZ-Cbfα1 signaling.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"171 ","pages":"Article 106152"},"PeriodicalIF":2.2,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142815241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CD10-positive cells on osteogenic differentiation of human maxillary/mandibular bone marrow-derived mesenchymal stem cells","authors":"Tomoaki Sakurai,&nbsp;Masakazu Ishii,&nbsp;Haruka Miyata,&nbsp;Nao Ikeda,&nbsp;Fumio Suehiro,&nbsp;Naohiro Komabashiri,&nbsp;Yurika Oura,&nbsp;Masahiro Nishimura","doi":"10.1016/j.archoralbio.2024.106135","DOIUrl":"10.1016/j.archoralbio.2024.106135","url":null,"abstract":"<div><h3>Objective</h3><div>This study was aimed at investigating the effect of CD10-positive cells within the maxillary/mandibular bone marrow-derived mesenchymal stem cells (MBMSCs) on osteogenic differentiation of MBMSCs.</div></div><div><h3>Design</h3><div>CD10 expression in iliac bone marrow-derived MSCs (IBMSCs), MBMSCs, and gingival fibroblasts was measured using flow cytometry. The osteogenic potential of 19 MBMSC lines was evaluated, and based on it, they were classified into osteogenic-High and osteogenic-Low groups. The percentage of CD10-positive cells in each group was compared. Effect of coculturing gingival fibroblasts and CD10-positive cells on the osteogenic potential of MBMSCs was also assessed. Expression of tissue inhibitor of metalloprotease-1 (TIMP-1) in osteogenic-High and osteogenic-Low MBMSCs was measured using quantitative real-time polymerase chain reaction, western blotting, and enzyme-linked immunosorbent assay. The molecular mechanisms underlying the regulation of osteogenic differentiation in MBMSCs were investigated.</div></div><div><h3>Results</h3><div>CD10 was not expressed in IBMSCs, but was highly expressed in fibroblasts. In MBMSCs, the CD10-positivity rate varied considerably between cells. MBMSCs with a high-CD10 positivity rate showed low osteogenic potential. Coculture with fibroblasts or CD10-positive cells reduced the osteogenic potential of MBMSCs. TIMP-1 was highly expressed in CD10-positive cells, and osteogenic-Low MBMSCs showed significantly higher TIMP-1 expression compared with osteogenic-High MBMSCs. β-catenin signaling was suppressed in osteogenic-Low MBMSCs.</div></div><div><h3>Conclusion</h3><div>This study revealed that TIMP-1 secreted from CD10-positive cells may be involved in the suppression of the osteogenic potential of MBMSCs by contamination with CD10-positive cells. This finding provides important insights for developing bone regeneration therapies using MBMSCs.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106135"},"PeriodicalIF":2.2,"publicationDate":"2024-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salivary alpha amylase as a diagnostic biomarker for dental caries – A systematic review and meta analysis","authors":"S. Savitha Priyadarsini ,&nbsp;P.G. Naveen Kumar ,&nbsp;Mahesh R. Khairnar ,&nbsp;Zainab Akram ,&nbsp;Ridhi Ghodela ,&nbsp;Sachin Kumar Jadhav ,&nbsp;Neha Shukla","doi":"10.1016/j.archoralbio.2024.106136","DOIUrl":"10.1016/j.archoralbio.2024.106136","url":null,"abstract":"<div><h3>Objective</h3><div>This systematic review and meta-analysis aimed to assess the association between the dental caries and the level of salivary alpha amylase activity among caries active and caries free individuals.</div></div><div><h3>Design</h3><div>This review followed the PRISMA guidelines and registered in PROSPERO (CRD42024518973). A comprehensive search across various research databases until January 31, 2024 yielded 1031 articles, where multiple screening ultimately narrowed it to 13 articles. Appropriate cross sectional studies comparing caries active (CA), caries free (CF) group and evaluating their level of salivary alpha amylase activity were included.</div></div><div><h3>Results</h3><div>The meta-analysis was performed on all 13 included studies utilizing a random effects model and two sub group analysis were performed where studies with salivary alpha amylase activity estimated using spectrophotometer and autoanalyzer were analyzed separately among caries active and caries free group. The pooled data reveals that the salivary alpha amylase activity was significantly higher (p = 0.005) in caries active group compared to caries free group with a standardized mean difference of 1.30.</div></div><div><h3>Conclusion</h3><div>This review indicated the differences in the salivary alpha amylase activity among caries active and caries free individuals which could aid in identifying individuals susceptible to dental caries thereby offering new insights for preventive dentistry by assessing their caries risk, enabling tailored preventive strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106136"},"PeriodicalIF":2.2,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142746110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parental transmission effects of the IRF6 polymorphisms among Non-Syndromic Cleft Lip with or without cleft palate in Kerala case parent trios","authors":"Reema Rose Alappat ,&nbsp;Sunish Kadayil Sachith ,&nbsp;Pulikkottil Raphael Varghese ,&nbsp;Puthucode V. Narayanan ,&nbsp;Alex George","doi":"10.1016/j.archoralbio.2024.106134","DOIUrl":"10.1016/j.archoralbio.2024.106134","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this study was to evaluate the <em>IRF6</em> gene polymorphisms in the etiology of the Non-syndromic Orofacial Cleft (NSOFC) population from Kerala.</div></div><div><h3>Design</h3><div>The study included a hundred case-parent triads of NSOFC from the Kerala Population. Genomic DNA was isolated from the trios. The polymorphisms rs2235371 and rs7552506 were genotyped using polymerase chain reaction (PCR) and the Sanger sequencing method. PLINK and haploview software were used for the statistical analysis.</div></div><div><h3>Results</h3><div>We found a substantial association of the rs2235371 (p-value = 0.0094, OR = 2.2) with NSOFC. The allelic TDT analysis ‘A’ allele of rs2235371 showed significant paternal transmission (p-value = 0.03, P_POO=0.506), whereas the ‘C’ allele of rs7552506 (p-value = 0.0094, P_POO=0.0527) showed an excess maternal transmission. The linkage disequilibrium (LD) values (D’= 0.83 and r² =0.033) between rs2235371 and rs7552506 discovered that these two SNPs are not in strong LD. Haplotype A-G exhibited a significantly reduced risk for oral clefts (p=0.035).</div></div><div><h3>Conclusion</h3><div>The current study revealed that polymorphism rs2235371 is associated with NSOFC. The rs2235371 appears to be significant with the trait in the paternal lineage but not when considering both lineages together. On the other hand, rs7552506 shows a significant association in the maternal lineage, with the pooled analysis approaching statistical significance. This study indicates that the <em>IRF6</em> gene may be a risk factor for NSOFC in the Kerala population.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106134"},"PeriodicalIF":2.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between estrogen receptors polymorphisms and temporomandibular joint disorders: A systematic review","authors":"Giusy Rita Maria La Rosa ,&nbsp;Carla Loreto ,&nbsp;Eugenio Pedullà ,&nbsp;Claudia Lombardo","doi":"10.1016/j.archoralbio.2024.106130","DOIUrl":"10.1016/j.archoralbio.2024.106130","url":null,"abstract":"<div><h3>Objectives</h3><div>This systematic review aimed to investigate the association between genetic polymorphisms in estrogen receptor alpha (ERα) and beta (ERβ) and the presence and severity of temporomandibular disorders (TMDs).</div></div><div><h3>Materials and methods</h3><div>A comprehensive search was conducted in PubMed, Scopus and Web of Science on October 19, 2024, without language or time restrictions. Studies were included if they were cross-sectional, case-control, or cohort designs and reported data on ER-polymorphisms and TMDs, diagnosed using validated criteria. Two independent reviewers screened the results to identify eligible studies. Data were extracted and synthesized narratively due to high heterogeneity across included studies, with a subgroup analysis performed to evaluate the influence of sex. The risk of bias was assessed using the Joanna Briggs Institute's critical appraisal tools.</div></div><div><h3>Results</h3><div>The search identified 277 articles, of which 8 studies met the inclusion criteria. Seven were case-control and two cross-sectional studies. The most commonly investigated ER polymorphisms were ESR1 (Pvu II and Xba I) and ESR2 (rs1676303). Some studies identified associations between specific polymorphisms and TMD-related pain or joint conditions, although results varied across different populations and subtypes of TMDs.</div></div><div><h3>Conclusions</h3><div>The findings suggest potential associations between specific ERα and ERβ polymorphisms and TMD risk, particularly in women. However, inconsistencies across studies underscore the need for prospective and larger studies to confirm these genetic links and clarify their clinical significance.</div></div><div><h3>Systematic Review Registration</h3><div>CRD42024581266</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106130"},"PeriodicalIF":2.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergistic potential of Bauhinia holophylla leaf extracts with conventional antifungals in the inhibition of Candida albicans: A new approach for the treatment of oral candidiasis","authors":"Stéfani de Oliveira Rosa ,&nbsp;Adriana Araújo de Almeida-Apolonio ,&nbsp;João Víctor de Andrade dos Santos ,&nbsp;Cleison da Rocha Leite ,&nbsp;Wellinton Jhon Cupozak-Pinheiro ,&nbsp;Claudia Andréa Lima Cardoso ,&nbsp;Thiago Luis Aguayo de Castro ,&nbsp;Deisiany Gomes Ferreira ,&nbsp;Melyssa Negri ,&nbsp;Kelly Mari Pires de Oliveira","doi":"10.1016/j.archoralbio.2024.106133","DOIUrl":"10.1016/j.archoralbio.2024.106133","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigated the combination of <em>Bauhinia holophylla</em> (Bong.) Steud. leaf extracts with conventional antifungal agents, highlighting the extracts' potential as adjuvants in treating oral candidiasis.</div></div><div><h3>Design</h3><div>Ethanolic and aqueous extracts of <em>B. holophylla</em> leaves were analyzed using ultra-high-performance liquid chromatography with a diode array detector (UHPLC-DAD) to assess their chemical composition. Their Minimum Inhibitory Concentration (MIC) against standard strains of <em>Candida albicans</em> and isolates from oral mucosa was determined. Additionally, the potential synergistic effects with chlorhexidine gluconate, nystatin, and fluconazole were investigated, along with their impact on inhibiting and disrupting biofilm formation, germ tube formation of <em>C. albicans</em>, and cytotoxicity in human erythrocytes.</div></div><div><h3>Results</h3><div>Protocatechuic acid, epicatechin, and rutin were identified in both extracts. They exhibited fungistatic activity with a median minimum inhibitory concentration (MIC₅₀) of 15.62 µg/mL for the ethanolic extract (EEB) and 62.50 µg/mL for the aqueous extract (AEB) against <em>C. albicans</em>. In growth kinetics, both extracts reduced the viable cell count of <em>C. albicans</em> by 2 logs after 24 h compared to the positive control. The extracts reduced germ tube formation by 81.6 % for EEB and 86.3 % for AEB. The synergistic combination with fluconazole and nystatin resulted in a 50 % reduction in the concentration required to inhibit <em>C. albicans</em> growth. No hemolytic activity was detected in human erythrocytes at the tested concentrations.</div></div><div><h3>Conclusion</h3><div>Both ethanolic and aqueous extracts show promising potential as adjuvants in managing oral candidiasis. Notably, the aqueous extract is advantageous due to its non-toxic solvent, cost-effectiveness, and ease of preparation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106133"},"PeriodicalIF":2.2,"publicationDate":"2024-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142702150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}