Archives of oral biology最新文献

{"title":"In vitro evidence that Antiretroviral Therapy (ART) enhances protection against dental erosion in People Living with HIV (PLHIV) and individuals on Pre-Exposure Prophylaxis (PrEP)","authors":"Nayara Flores Macedo ,&nbsp;Tommy Baumann ,&nbsp;Marília Afonso Rabelo Buzalaf ,&nbsp;Luciana Reis Azevedo Alanis ,&nbsp;Thiago Saads Carvalho","doi":"10.1016/j.archoralbio.2025.106314","DOIUrl":"10.1016/j.archoralbio.2025.106314","url":null,"abstract":"<div><h3>Objective</h3><div>This study aimed to evaluate the protective capacity of the salivary pellicle in people living with HIV (PLHIV) on antiretroviral therapy (ART) and individuals on pre-exposure prophylaxis (PrEP) for HIV.</div></div><div><h3>Material and methods</h3><div>Sixteen participants were divided into three groups: Control (n = 5; HIV-negative, not on ART), HIV (n = 7; PLHIV on ART), and PrEP (n = 4; HIV-negative, on PrEP). Stimulated saliva samples were pooled for each group. Enamel specimens (n = 45) were polished and allocated among the groups. Samples underwent five cycles of enamel pellicle formation (120 µL saliva, 37°C, 2 h), each followed by an erosive challenge (6 ml of 1 % citric acid, 25°C, 1 min, pH 3.6). Surface hardness (SH), surface reflection intensity (SRI), surface roughness (Sa), and calcium release (CaR) were measured before and after the erosive cycles. Statistical analyses included the Kolmogorov–Smirnov test for normality, one-way ANOVA for SH and SRI, and Kruskal–Wallis tests for Sa and CaR (α = 0.05).</div></div><div><h3>Results</h3><div>PrEP individuals exhibited the greatest protection against erosion in terms of SH, followed by PLHIV (p = 0.016). Both groups showed significantly better protection than the Control group, with higher SRI values (p &lt; 0.05) and lower enamel surface roughness (p = 0.001). No significant differences were observed among the groups regarding calcium release (p &gt; 0.05).</div></div><div><h3>Conclusion</h3><div>PLHIV and individuals on PrEP demonstrate enhanced protective properties of the salivary pellicle against dental erosion.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106314"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity in the bacterial communities of oral saliva from different genders of the Tibetan Population","authors":"Zhiyan Li ,&nbsp;Peixia Zhang ,&nbsp;Minhaoxue Zou ,&nbsp;Chong Zhao ,&nbsp;Mingxiang Wang ,&nbsp;Bohan Shi ,&nbsp;Derui Zhu","doi":"10.1016/j.archoralbio.2025.106315","DOIUrl":"10.1016/j.archoralbio.2025.106315","url":null,"abstract":"<div><h3>Objective</h3><div>This study aims to investigate gender-based differences in oral bacterial community structure and diversity among Tibetans in Guoluo Prefecture, Qinghai, China, in order to support future research on the prevention and treatment of oral and systemic diseases.</div></div><div><h3>Methods</h3><div>Oral saliva samples from 19 Tibetan males and 19 females were analyzed using Illumina high-throughput sequencing of the 16S rRNA gene. Species diversity indices and community composition were compared between genders.</div></div><div><h3>Results</h3><div>A total of 9556 Species Observed (Sobs) were collected, with 5264 Sobs from Tibetan males (14 phyla, 128 genera) and 4292 Sobs from females (13 phyla, 108 genera). Species diversity analyses revealed that the Shannon and ACE indices of the oral bacterial communities of Tibetan males (2.45–4.07, 202.29–406.01) were significantly higher (<em>P</em> &lt; 0.05) as compared to the females (1.77–3.55, 131.58–410.31). Community composition analysis demonstrated that the predominant phyla of the oral saliva samples were similar in Tibetan males and females and included <em>Firmicutes</em>, <em>Bacteroidota</em>, and <em>Actinobacteriota</em>. Gender differences were detected in the dominant oral bacterial genera, with <em>Neisseria</em>, <em>Rothia</em> and <em>Prevotella</em> being the genera exhibiting key differences. In Tibetan males, the dominant genera were <em>Streptococcus</em> (12.27 %–60.22 %), <em>Veillonella</em> (1.98 %–37.13 %), <em>Prevotella</em> (12.27 %–60.22 %), <em>Actinomyces</em> (1.07 %–25.41 %), <em>Rothia</em> (0.96 %–11.16 %), <em>Porphyromonas</em> (0.37 %–13.72 %), and <em>Gemella</em> (0.33 %–10.12 %). For females, the predominant genera included <em>Streptococcus</em> (11.49 %–54.24 %), <em>Neisseria</em> (0.46 %–49.22 %), <em>Rothia</em> (5.05 %–29.70 %), <em>Porphyromonas</em> (0.11 %–21.48 %), <em>Gemella</em> (1.01 %–13.31 %), <em>Granulicatella</em> (1.18 %–14.93 %), <em>Prevotella</em> (0.06 %–29.18 %), <em>Haemophilus</em> (0.41 %–6.97 %), and <em>Veillonella</em> (0.26 %–13.72 %).</div></div><div><h3>Conclusions</h3><div>Gender-specific variations in oral microbiota composition highlight potential implications for understanding oral and systemic disease susceptibility in Tibetan populations. These findings provide a foundation for targeted preventive and therapeutic strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106315"},"PeriodicalIF":2.2,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of PANoptosis activation in irreversible pulpitis: An in vitro study based on human tissue samples","authors":"Bo Yang ,&nbsp;YinYu Fu ,&nbsp;Ling Lai,&nbsp;Jun He,&nbsp;Xinzhu Li,&nbsp;Jin Hou","doi":"10.1016/j.archoralbio.2025.106305","DOIUrl":"10.1016/j.archoralbio.2025.106305","url":null,"abstract":"<div><h3>Objectives</h3><div>To investigate the activation of the PANoptosome in pulpitis by examining apoptosis, pyroptosis, and necroptosis within inflamed dental pulp tissue.</div></div><div><h3>Design</h3><div>This study reanalyzed an RNA-seq dataset from the GEO database and validated the results with qRT-PCR. Immunoblotting assessed the activation of apoptosis, pyroptosis, and necroptosis pathways in healthy and inflamed pulp tissues. Coimmunoprecipitation detected potential interactions between caspase-8 and PYCARD in pulpitis. We used scRNA-seq data to determine the distribution of caspase-8, PYCARD, and RIPK1 between the cell types, using R software.</div></div><div><h3>Results</h3><div>Several PANoptosis-related genes were found to be upregulated at the mRNA levels. However, only the activation of apoptosis and pyroptosis was detected in inflamed pulp. The interaction of caspase-8 and PYCARD in pulpitis was not detected. Reanalysis of single-cell RNA sequencing (scRNA-seq) data revealed that caspase-8 is mainly expressed in T cells, PYCARD is expressed in all cell types, particularly in monocytes, dendritic cells and plasma cells, while RIPK1 shows no specificity in its expression among different cell types.</div></div><div><h3>Conclusions</h3><div>Apoptosis and pyroptosis are activated in inflamed human pulp tissue. However, these two pathways do not manifest as PANoptosis; instead, they exist independently within different cell populations in the tissue.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"177 ","pages":"Article 106305"},"PeriodicalIF":2.2,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage depletion in male mice impairs extraction socket healing by disrupting bone regeneration and tissue repair","authors":"Kanji Horibe,&nbsp;Daisuke Nishida,&nbsp;Hiroaki Nakamura","doi":"10.1016/j.archoralbio.2025.106312","DOIUrl":"10.1016/j.archoralbio.2025.106312","url":null,"abstract":"<div><h3>Objectives</h3><div>Macrophages play a critical role in tissue repair and bone regeneration by regulating inflammation, angiogenesis, and osteoblast differentiation. The present study examined the role of macrophages in extraction socket healing using a clodronate liposome-induced macrophage depletion model in mice, with a focus on both soft and hard tissue regeneration. We also investigated the involvement of cytokines, such as TGF-β, PDGF, and BMP2.</div></div><div><h3>Design</h3><div>Male C57BL/6 J mice were intravenously administered clodronate or control liposomes three days prior to tooth extraction and the healing process was analyzed by histology, immunohistochemistry, RNA in situ hybridization, and quantitative RT-PCR.</div></div><div><h3>Results</h3><div>The clodronate liposome treatment significantly reduced F4/80-positive and CD206-positive macrophages, delayed mucosal closure, disrupted angiogenesis, and suppressed new bone formation. Vascular density in the extraction socket was significantly reduced, and was accompanied by decreases in the expression of PDGF-A and PDGF-B, which are critical for mesenchymal stem cell migration and angiogenesis. The expression of BMP2 and TGF-β and their downstream signaling were also suppressed, which impaired new bone formation.</div></div><div><h3>Conclusion</h3><div>These results demonstrate that macrophages are essential for the cytokine-mediated coordination of soft and hard tissue regeneration and highlight their therapeutic potential to improve periodontal and bone regeneration treatments. Because all experiments were performed in male C57BL/6 mice, these findings may not fully extend to females, in which estrogen is known to modulate macrophage function and bone healing.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106312"},"PeriodicalIF":2.2,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Brazilian green and brown propolis on human pulp cells and bacteria involved in primary endodontic infection: Ex Vivo study","authors":"Washington Henrique Themoteo da Silva ,&nbsp;Fernanda Rodrigues Guedes ,&nbsp;Caio Luiz Lins-Candeiro ,&nbsp;Camila Maura Morais Lima dos Santos ,&nbsp;Maria Anita Lemos Vasconcelos Ambrosio ,&nbsp;Rodrigo Cassio Sola Veneziani ,&nbsp;Jairo Kenupp Bastos ,&nbsp;Mariana Brentini Santiago ,&nbsp;Carlos Henrique Gomes Martins ,&nbsp;Ana Paula Turrioni","doi":"10.1016/j.archoralbio.2025.106294","DOIUrl":"10.1016/j.archoralbio.2025.106294","url":null,"abstract":"<div><h3>Objectives</h3><div>This study evaluated the antibacterial effects and impact of Brazilian green propolis (BGP) and brown propolis (BBP) on human dental pulp fibroblasts (HDPF).</div></div><div><h3>Design</h3><div>HDPF were seeded in 96-well plates (10,000 cells/well) and exposed to BGP (5, 10, 50 μg/mL), BBP (5, 10, 50 μg/mL), 0.5 % dimethyl sulfoxide, 0.018 % carbamide peroxide, 10 μg/mL tumor necrosis factor alpha (TNF-α), and Dulbecco's Modified Eagle Medium (control). Cell viability (methyl-thiazol-tetrazolium assay), reactive oxygen species (ROS, DCFH-DA probe), nitric oxide (NO; Griess reagent), and cell morphology (scanning electron microscopy) were analyzed. Wound healing was assessed at 0, 24, and 48 h. The minimum inhibitory concentration/minimum bactericidal concentration was determined. Dunn’s rank-sum test (5 % significance) was used.</div></div><div><h3>Results</h3><div>BGP and BBP exhibited high cytocompatibility, maintained cell viability, and did not induce NO production (p &gt; 0.05). ROS production in the TNF-α group differed considerably from that in the other groups. Regarding the antibacterial effect at concentrations ≤ 100 μg/mL, BGP was effective against <em>P. micra</em>, while BBP was effective against seven bacterial species.</div></div><div><h3>Conclusions</h3><div>BGP and BBP demonstrated high cytocompatibility and absence of ROS and NO production, with BGP promoting greater HDPF stimulation and BBP exhibiting superior antibacterial activity.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106294"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bidirectional interaction between epithelial-mesenchymal transition and PD-1/PD-L1 expression in tongue carcinogenesis","authors":"Hannah Gil de Farias Morais ,&nbsp;Helder Domiciano Dantas Martins ,&nbsp;Alexandre Rolim da Paz ,&nbsp;Everton Freitas de Morais ,&nbsp;Paulo Rogerio Ferreti Bonan ,&nbsp;Roseana de Almeida Freitas","doi":"10.1016/j.archoralbio.2025.106304","DOIUrl":"10.1016/j.archoralbio.2025.106304","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate, through immunohistochemistry, the interaction between epithelial-mesenchymal transition (EMT) and the immune checkpoint proteins PD-1 and PD-L1 in oral tongue squamous cell carcinoma (OTSCC).</div></div><div><h3>Design</h3><div>The EMT status was determined based on the immunostaining of E-cadherin and N-cadherin proteins. EMT status and the immunoexpression of PD-L1 and PD-1 were analyzed semiquantitatively in 61 cases of OTSCC, and the cases were categorized into low and high expression groups for association with clinicopathological variables and overall survival (OS) and disease-free survival (DFS) rates.</div></div><div><h3>Results</h3><div>A predominance of cases with positive EMT status was identified (75.4 %), with a statistically significant association between histological grade of malignancy and the occurrence of EMT (<em>p</em> = 0.003). Regarding the clinical parameters analyzed, a statistically significant difference was observed between OTSCC cases with larger sizes and higher levels of PD-L1 expression (<em>p</em> = 0.048). Additionally, the immunoexpressions of the PD-1 and PD-L1 proteins were higher in cases with positive EMT status (<em>p</em> = 0.003 and <em>p</em> = 0.001, respectively), as well as a moderate, positive, and statistically significant correlation between the immunoexpression scores of PD-1 and PD-L1 proteins (r = 0.571; <em>p</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>The results of the present study suggest the potential involvement of EMT in the modulation of an immunosuppressive microenvironment in OTSCC.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106304"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of xanthan gum-based, guar gum-based, and starch-based thickeners on tongue pressure and pharyngeal residue","authors":"Jumpei Okawa ,&nbsp;Yuki Hayakawa ,&nbsp;Ayako Ito ,&nbsp;Satomi Kawakami ,&nbsp;Kazuhiro Miyaji ,&nbsp;Takahiro Ono ,&nbsp;Kazuhiro Hori","doi":"10.1016/j.archoralbio.2025.106303","DOIUrl":"10.1016/j.archoralbio.2025.106303","url":null,"abstract":"<div><h3>Objective</h3><div>To examine the relationship between pharyngeal residue and both the oral and pharyngeal stages by observing tongue pressure and pharyngeal dynamics during the swallowing of thickened liquid prepared with starch-based, guar gum-based, and xanthan gum-based thickeners.</div></div><div><h3>Design</h3><div>The participants were 20 volunteers without dysphagia (17 male, 3 female, 33.1 ± 8.8 years). Samples were water and liquids thickened with starch-based, guar gum-based, and xanthan gum-based thickeners, and each was 15 mL. The shear viscosity of thickened liquids was 400 mPa·s at 50 s-1. Whiteout duration and pharyngeal residue were observed by videoendoscopy, and swallowing sounds were recorded with a microphone. Tongue pressure was measured by using a multichannel sensor sheet placed on the palate. Whiteout duration, swallowing sound duration, and tongue pressure parameters were compared between the samples and the presence of pharyngeal residue.</div></div><div><h3>Results</h3><div>The frequency of pharyngeal residue was significantly higher with starch-based samples than with water and xanthan-based samples (<em>P</em> &lt; 0.05). The swallowing sound duration was significantly shorter for all of the thickened samples compared with water (<em>P</em> &lt; 0.05). The tongue pressure at the posterior-circumferential for starch-based and guar gum-based samples was found to be significantly different from that for water (<em>P</em> &lt; 0.05). Furthermore, when pharyngeal residue occurred, tongue pressure was significantly greater (<em>P</em> &lt; 0.01), and the whiteout duration and swallowing sound duration were significantly longer (<em>P</em> &lt; 0.05, <em>P</em> &lt; 0.01, respectively).</div></div><div><h3>Conclusion</h3><div>Our findings suggest that the type of thickener influences both the oral and pharyngeal stages, which are associated with the occurrence of pharyngeal residue.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106303"},"PeriodicalIF":2.2,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Necroptosis and its role in the pathogenesis and treatment of temporomandibular joint osteoarthritis: A narrative review","authors":"Miaomiao Zhao ,&nbsp;Luyao Song ,&nbsp;Zhichao Liu,&nbsp;Yuting Li,&nbsp;Yingnan Wang","doi":"10.1016/j.archoralbio.2025.106300","DOIUrl":"10.1016/j.archoralbio.2025.106300","url":null,"abstract":"<div><h3>Objective</h3><div>This review aimed to explore the role of necroptosis in the pathogenesis and treatment of temporomandibular joint osteoarthritisjoints. We sought to elucidate the mechanisms by which necroptosis contributes to arthritis development and identify potential therapeutic targets within the necroptosis pathway.</div></div><div><h3>Design</h3><div>An electronic literature search was conducted in PubMed, Scopus, EBSCO, ProQuest, ScienceDirect, and Springer for studies on necroptosis, arthritis, and temporomandibular joint osteoarthritis published between 2004 and 2024.</div></div><div><h3>Results</h3><div>Necroptosis, a programmed cell death pathway characterized by the activation of receptor-interacting protein kinase 1/3, leads to cell membrane rupture and the release of damage-associated molecular patterns, which are instrumental in promoting inflammation. This review highlights the involvement of necroptosis in the progression of multiple types of arthritis, especially temporomandibular joint osteoarthritis. These findings highlight the potential of necroptosis inhibitors as therapeutic agents, with several small-molecule inhibitors demonstrating efficacy in preclinical models of arthritis.</div></div><div><h3>Conclusions</h3><div>Necroptosis is a significant factor in the pathogenesis of arthritis, particularly temporomandibular joint osteoarthritis, and represents a promising therapeutic target. Targeting the necroptosis pathway may offer a novel approach for managing joint damage and osteochondral inflammation. Further research is necessary to fully understand the mechanisms of necroptosis in temporomandibular joint osteoarthritis and to develop targeted therapies for clinical application.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106300"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144146798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of optimised sample preparation method on DNA recovery from subgingival plaque in a population with periodontitis","authors":"Pasquale Santamaria,&nbsp;Mandeep Ghuman,&nbsp;Luigi Nibali","doi":"10.1016/j.archoralbio.2025.106299","DOIUrl":"10.1016/j.archoralbio.2025.106299","url":null,"abstract":"<div><h3>Objective(s)</h3><div>This study aimed to evaluate whether an alternate sample preparation method could enhance DNA yield from subgingival plaque samples collected from patients with untreated periodontitis.</div></div><div><h3>Design</h3><div>Fifty-six consecutive participants with untreated periodontitis each provided two subgingival plaque samples from periodontal sites with probing pocket depth (PPD) &gt; 5 mm. In the alternate method (AM), 1.4 mm ceramic beads were added to the sample prior to supernatant removal to protect the microbial pellet, after which samples were processed using a standardized extraction protocol and subjected to 16S rRNA gene sequencing (V3–V4 region).</div></div><div><h3>Results</h3><div>A total of 112 samples were analysed. DNA concentration (ng/µl) was significantly higher in the AM group (23.82 ± 23.31) compared to the standard method (SM) group (13.6 ± 17.07; p &lt; 0.001). The DNA input in 2.5 µl was also significantly greater in the AM group (1156 ± 1139 ng vs. 277.96 ± 273.12 ng; p &lt; 0.001). Shannon diversity was significantly higher in AM than SM (p = 6e–04). No significant differences in DNA yield or microbial diversity were observed between the two periodontal sites within each group.</div></div><div><h3>Conclusion</h3><div>The alternate sample preparation method improved DNA concentration compared to standard sample preparation method of sub-gingival plaque analysis collected from patients with untreated periodontitis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106299"},"PeriodicalIF":2.2,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144189715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chondroprotective role of histone deacetylase 4 in early-stage of temporomandibular joint osteoarthritis in male rats","authors":"Qi Ning ,&nbsp;Weihua Han ,&nbsp;Juanhong Meng ,&nbsp;Yehua Gan","doi":"10.1016/j.archoralbio.2025.106301","DOIUrl":"10.1016/j.archoralbio.2025.106301","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to investigate the expression and role of histone deacetylase 4 (<em>Hdac4</em>) in temporomandibular joint osteoarthritis in rats.</div></div><div><h3>Design</h3><div>A model of temporomandibular joint (TMJ) osteoarthritis was established by partial discectomy in male rats. In the early stage of TMJ osteoarthritis, small interfering-HDAC4 (si-HDAC4) with adeno-associated virus was injected into the rat articular cavity to knockdown the expression of <em>Hdac4</em> in TMJ. And an adenoviral vector was used to transfer <em>Hdac4</em> into the rat articular cavity to increase the level of <em>Hdac4</em> in TMJ. Subsequently, the pathological changes and stages of condylar cartilage were examined histopathologically at different time points after injection respectively, and the microstructure of TMJ subchondral bone was evaluated using micro computed tomography.</div></div><div><h3>Results</h3><div><em>Hdac4</em> expression in condylar cartilage was upregulated in the early-stage of TMJ osteoarthritis, but decreased in the mid to late-stage. Knockdown of <em>Hdac4</em> expression in the early-stage of TMJ osteoarthritis accelerated the destruction of condylar cartilage in vivo, while overexpression of <em>Hdac4</em> reduced the destruction of cartilage in the late-stage. Meanwhile, on micro computed tomography, the microstructure of subchondral bone in TMJ showed that overexpression of <em>Hdac4</em> led to a decrease in incidence of bone sclerosis.</div></div><div><h3>Conclusions</h3><div>Our results suggested that <em>Hdac4</em> has a certain chondroprotective effect in the development of TMJ osteoarthritis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"176 ","pages":"Article 106301"},"PeriodicalIF":2.2,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144167588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}