Archives of oral biology最新文献

{"title":"Endoplasmic reticulum stress as a nexus of temporomandibular joint osteoarthritis","authors":"Xinqi Huang ,&nbsp;Zinan Cen ,&nbsp;Xinxuan Zhou ,&nbsp;Zhihe Zhao ,&nbsp;Xiao Cen","doi":"10.1016/j.archoralbio.2025.106407","DOIUrl":"10.1016/j.archoralbio.2025.106407","url":null,"abstract":"<div><h3>Objective</h3><div>This review aims to summarize the molecular architecture of endoplasmic reticulum (ER) stress signaling networks and their mechanistic involvement in temporomandibular joint osteoarthritis (TMJOA) progression, and current therapeutic strategies targeting ER stress mediators and the obstacles from bench to bedside.</div></div><div><h3>Design</h3><div>The related literatures of the roles of ER in TMJOA were searched through PubMed database by different combinations of the following keywords including animal models, ER, unfolded protein response (UPR), ER-associated degradation (ERAD), ER-phagy, TMJ, and OA. No filters were used in the search. The references of eligible studies were also analyzed and reviewed comprehensively.</div></div><div><h3>Results</h3><div>This review discussed how ER stress signaling orchestrated TMJOA pathogenesis, including UPR, ERAD, and ER-phagy. It was also summarized how biomechanical stress and hypoxic microenvironment synergistically exacerbated ER stress, and the current therapeutic strategies for TMJOA based on ER stress modulators and the obstacles in bench-to-bedside research.</div></div><div><h3>Conclusions</h3><div>ER proteostasis represented a pivotal but underexplored therapeutic axis in TMJOA. Bridging the gap between mechanistic understanding of ER stress adaptation and TMJ-specific pathobiology is essential for developing novel therapeutic strategies for TMJOA.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106407"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145202279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced regenerative potential of human dental pulp stem cells for the pulp-dentin complex through coculture with iPSC-derived endothelial cells: An in vitro study","authors":"Hsu Myat Cho ,&nbsp;Ukseong Kim ,&nbsp;Sunil Kim ,&nbsp;Stephanie Myeong Choi ,&nbsp;Sukjoon Lee ,&nbsp;Euiseong Kim","doi":"10.1016/j.archoralbio.2025.106409","DOIUrl":"10.1016/j.archoralbio.2025.106409","url":null,"abstract":"<div><h3>Objectives</h3><div>Although cell-based therapies using human dental pulp stem cells (hDPSCs) with other cell lineages and growth factors show promise in regenerative endodontics, combining hDPSCs with induced pluripotent stem cell-derived endothelial cells (iPSC-ECs) is unexplored. Moreover, iPSC-ECs overcome ethical and practical challenges related to primary endothelial cells. This study explored the odontogenic and angiogenic potential of hDPSCs and iPSC-ECs in direct coculture.</div></div><div><h3>Design</h3><div>hDPSCs were isolated from extracted human teeth, and iPSC‑ECs were generated via episomal reprogramming of hDPSCs followed by endothelial differentiation. Four groups were established for differentiation assays: hDPSCs in basal medium, osteogenic medium, modified osteogenic medium (D‑MOD), and coculture with iPSC‑ECs (1:5) in D‑MOD. Mineralization was assessed by alkaline phosphatase and alizarin red S staining; gene expression of odontogenic (<em>DSPP, IBSP, ALPL</em>) and angiogenic (<em>PECAM1, MCAM, KDR</em>) markers was measured by RT‑qPCR; protein levels were evaluated by Western blot and nestin immunofluorescence; and angiogenic capacity in the D‑MOD and coculture groups was quantified via Matrigel tube‑formation assay.</div></div><div><h3>Results</h3><div>The coculture group showed enhanced mineralization and significantly increased expression of <em>DSPP</em>, <em>IBSP</em>, and <em>PECAM1</em>. Protein analysis confirmed elevated DSPP and nestin levels. Tube formation assays revealed significantly more junctions, segments, and meshes in the coculture group.</div></div><div><h3>Conclusions</h3><div>This study demonstrated <em>in vitro</em> that coculturing hDPSCs with iPSC-ECs enhances both odontogenic and angiogenic differentiation compared to hDPSCs cultured alone. These findings highlight the potential of iPSC technology in regenerative endodontics and indicate a promising cell-based approach for future therapeutic applications.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106409"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145245253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B. lactis HN019 modulates periapical inflammation and enhances cementum repair by increasing CEMP-1 expression in an animal model","authors":"Lisa Danielly Curcino Araujo ,&nbsp;Ana Paula Gomes-Moura ,&nbsp;Raquel Assed Bezerra da Silva ,&nbsp;Michel Reis Messora ,&nbsp;Flávia Aparecida Chaves Furlaneto ,&nbsp;Sérgio Luis de Souza Salvador ,&nbsp;Allan Radaic ,&nbsp;Pachiyappan Kamarajan ,&nbsp;Yvonne L. Kapila ,&nbsp;Paulo Nelson-Fillho ,&nbsp;Higinio Arzate ,&nbsp;Samin Sirous ,&nbsp;Geeta Bhuvanagiri ,&nbsp;Léa Assed Bezerra da Silva","doi":"10.1016/j.archoralbio.2025.106420","DOIUrl":"10.1016/j.archoralbio.2025.106420","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the effect of the HN019 probiotic on the modulation of inflammation and the resorption of mineralized tissues in experimentally induced periapical lesions in an animal model, evaluating its impact on the expression of cementum protein 1 (CEMP-1).</div></div><div><h3>Design</h3><div>Periapical lesion was induced in 45 Wistar rats. The animals were divided into groups according to the irrigating solution used. Root canals were irrigated on days 7 and 14. After 21 days, the animals were euthanized, and mandibles were processed for histological analysis. Descriptive and semi-quantitative evaluations of the inflammatory infiltrate, periodontal ligament, bone and cementum resorption, as well as counts of inflammatory cells were performed using HE staining. Also, to assess cementum formation, tissue sections were stained with an antibody for CEMP-1 and analyzed by immunohistochemistry.</div></div><div><h3>Results</h3><div>In the Infection + Probiotic Group, the inflammatory infiltrate was mixed and diffuse, ranging from mild to severe, with no statistically significant difference compared to the Control Group (p &gt; 0.05). However, in the Infection + Probiotic Group, cementum resorption was significantly lower compared to Control Group (p &lt; 0.001). Moreover, CEMP-1 expression was significantly higher in Infection + Probiotic-Group compared with all other groups (p &lt; 0.0001).</div></div><div><h3>Conclusion</h3><div>The probiotic HN019 significantly increased CEMP-1 expression, indicating cementum formation in periapical lesions in vivo. These findings suggest that <em>B. lactis</em> HN019 may have regenerative potential in clinical scenarios. Furthermore, this strain appears to modulate the inflammatory state of periapical lesions toward a reparative phenotype, characterized by reduced bone resorption. Also, inflammatory infiltrate was like control group.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106420"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145294648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hsa_circ_0007349 upregulates glutamate-ammonia ligase to modulate gingival fibroblast wound healing","authors":"Liang Ren ,&nbsp;Linlin He ,&nbsp;Runyu Zhang ,&nbsp;Xiaoyi Li ,&nbsp;Shimeng Xiao ,&nbsp;Shujuan Guo ,&nbsp;Chengcheng Liu ,&nbsp;Yi Ding","doi":"10.1016/j.archoralbio.2025.106419","DOIUrl":"10.1016/j.archoralbio.2025.106419","url":null,"abstract":"<div><h3>Objectives</h3><div>Human gingival fibroblasts (HGFs) are important cells that maintain the structure and integrity of periodontal tissues. Circular RNAs (circRNAs) play critical roles in cellular processes of periodontitis; however, their roles in HGFs remain unclear. This study aimed to elucidate the function and mechanism of a novel circRNA, hsa_circ_0007349, in periodontitis progression by validating its existence, establishing its role in a ceRNA network (targeting miR-127–5p/GLUL), assessing its regulatory effects on HGF functions in vitro, and confirming its pathological impact in a murine periodontitis model.</div></div><div><h3>Design</h3><div>We performed circRNA sequencing to identify differentially expressed circRNAs between periodontitis and healthy gingival tissues. We selected hsa_circ_0007349, and confirmed its circular structure by Sanger sequencing, RNase R and actinomycin D assays. hsa_circ_0007349/miR-127–5p/glutamate-ammonia ligase (GLUL) interactions were explored using bioinformatics analysis, dual-luciferase reporter assays, RT-qPCR, and western blotting, and their effects on HGF functions were assessed via transcriptome sequencing, Cell Counting Kit-8, cell scratch, and Transwell assays. To further investigate the pathogenic mechanisms, we established a standardized murine periodontitis model using female C57BL/6 mice, followed by histopathological evaluation of periodontal tissue destruction.</div></div><div><h3>Results</h3><div>Hsa_circ_0007349 expression significantly increased in gingival tissues of patients with periodontitis and in HGFs treated with <em>Porphyromonas gingivalis</em> lipopolysaccharide. hsa_circ_0007349 regulates miR-127–5p to affect cell proliferation, migration, and wound healing, and affects extracellular matrix metabolism and periodontal tissue damage via GLUL.</div></div><div><h3>Conclusion</h3><div>Hsa_circ_0007349 acts as a competing endogenous RNA to promote HGF proliferation, migration, wound healing and extracellular matrix turnover by targeting miR-127–5p and GLUL.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106419"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145263629","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mirt2 alleviates LPS-induced inflammation of osteoblasts in alveolar bone destruction","authors":"Yajie Wu ,&nbsp;Zhifei Su ,&nbsp;Bowen Zhang ,&nbsp;Lei Cheng ,&nbsp;Ziyou Wang ,&nbsp;He Yuan ,&nbsp;Jiyao Li","doi":"10.1016/j.archoralbio.2025.106410","DOIUrl":"10.1016/j.archoralbio.2025.106410","url":null,"abstract":"<div><h3>Objectives</h3><div>The long noncoding RNA (lncRNA) myocardial infarction–associated transcript 2 (Mirt2) has been confirmed to affect several inflammatory diseases. This study was conducted to explore the functional mechanism of Mirt2 in inflammatory alveolar bone loss and its possibility of being a therapeutic target.</div></div><div><h3>Design</h3><div>The expression level and potential role of Mirt2 in chronic inflammatory alveolar bone loss in mouse models of periodontitis and periapical periodontitis were investigated using micro-CT and qPCR. The characteristics of Mirt2 were evaluated by FISH, qPCR, ELISA, and alkaline phosphatase staining to confirm its function and mechanism of action in inflammatory response.</div></div><div><h3>Results</h3><div>Mirt2 expression was significantly enriched in inflammatory alveolar bone diseases. Mirt2 expression increased upon LPS stimulation in MC3T3-E1 cells (<em>P &lt; 0.05</em>), located at the cell cytoplasm. Mirt2 knockdown exacerbated the LPS-stimulated inflammatory response in MC3T3-E1 cells, whereas Mirt2 overexpression attenuated this effect and rescued LPS-impaired osteogenic differentiation.</div></div><div><h3>Conclusions</h3><div>lncRNA Mirt2 suggests a potential role in chronic inflammation–related bone loss, providing potential therapeutic target worthy of future investigation for inflammation-related bone loss.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106410"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145264037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular and thermal signatures of dental tissues in third molars: An in vitro comparative study using fourier-transform infrared spectroscopy and differential scanning calorimetry","authors":"Rola Zahedah ,&nbsp;Recep Üstünsoy ,&nbsp;Aliye Tuğçe Gürcan ,&nbsp;Bircan Dinç","doi":"10.1016/j.archoralbio.2025.106402","DOIUrl":"10.1016/j.archoralbio.2025.106402","url":null,"abstract":"<div><h3>Objective</h3><div>To characterize and compare the molecular and thermal characteristics of enamel, dentin, cementum, and the dentin–pulp complex in permanent third molars using Fourier-transform infrared (FTIR) spectroscopy and differential scanning calorimetry (DSC).</div></div><div><h3>Design</h3><div>Samples from extracted third molars (n = 15) were prepared and analyzed using FTIR to assess molecular composition and DSC to evaluate thermal transitions, including dehydration, collagen degradation, and mineral phase transformation. All measurements were conducted in triplicate.</div></div><div><h3>Results</h3><div>FTIR revealed enamel as highly mineralized with minimal organic content, dentin and cementum as collagen-rich, and the dentin–pulp complex as a hybrid tissue. DSC analysis identified consistent thermal transitions: water loss (110–125 °C), collagen breakdown (300–320 °C), and mineral decomposition (455–470 °C). Enamel displayed the highest crystallinity, while cementum exhibited the highest enthalpy change. Tissues with stronger FTIR collagen peaks corresponded to higher DSC energy release during protein degradation.</div></div><div><h3>Conclusion</h3><div>Molecular and thermal profiling of dental tissues provide baseline reference data for biomaterial design and regenerative strategies.</div></div><div><h3>Clinical significance</h3><div>Understanding tissue-specific molecular and thermal properties can guide the development of biomimetic restorative materials, inform safer thermal thresholds during clinical procedures, and support diagnostic approaches for aging and pathological changes.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106402"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145152422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Topical effect of polysaccharide cashew gum on the mitigation of dentin erosion progression under in vitro supraesophageal reflux simulation","authors":"Thaysa Fernandes Pinto Mendes ,&nbsp;Adrianne Caroline Pereira dos Santos Lima ,&nbsp;Eva Aline Costa Cutrim ,&nbsp;Paulo Sérgio de Araújo Sousa ,&nbsp;Durcilene Alves da Silva ,&nbsp;Lucas Antonio Duarte Nicolau ,&nbsp;Leily Macedo Firoozmand","doi":"10.1016/j.archoralbio.2025.106427","DOIUrl":"10.1016/j.archoralbio.2025.106427","url":null,"abstract":"<div><h3>Objective</h3><div>To evaluate, <em>in vitro</em>, the topical effect of polysaccharide cashew gum (PLS-cg) on dentine subjected to erosive cycles that simulate refluxate contact in the oral cavity.</div></div><div><h3>Design</h3><div>Human dentine blocks were prepared, standardized, and randomly assigned to one of three treatment groups (<em>n</em> = 10): control group (C) without treatment, commercial dental varnish (CDV) 5 % NaF fluoride varnish, and PLS-cg extract (experimental group). The baseline enamel superficial microhardness (HK) and roughness (Ra) were assessed. The dentin was subjected to erosive cycles (6 × 5 min/day for 9 days) using a hydrochloric acid–pepsin solution (pH 2; 0.75-mg/mL pepsin). Surface roughness (Ra), microhardness (HK), percentage surface hardness loss (%SHL), and topographic analysis with scanning electron microscopy (SEM) were used to evaluate the protective heteropolysaccharide effect. Predictive molecular docking simulations were used to investigate possible interactions between PLS-cg and metalloproteinases (MMP2 and MMP9). The data were analyzed with two-way analysis of variance, <em>t</em> test, Pearson’s correlation, and Tukey’s test (<em>p</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>PLS-cg preserved HK significantly better than the control group (<em>p</em> &lt; 0.001), with the lowest %SHL (-14.04 ± 8.22) and minimal Ra changes (0.124 ± 0.005). Pearson’s correlation between Ra and %SHL was significant and positive (0.7740, <em>p</em> &lt; 0.001). SEM analysis revealed more prominent dentinal tubule openings in the control group, whereas CDV and PLS-cg showed tubule obliteration. Molecular docking identified possible interactions between PLS-cg and MMPs.</div></div><div><h3>Conclusion</h3><div>The findings indicate that PLS-cg may reduce dentine erosion caused by supraesophageal manifestations of gastroesophageal reflux disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106427"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145318937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial effects of charcoal and fluoride dentifrices in oral biofilms","authors":"Ashley N. Bowers ,&nbsp;Caroline Coradi Tonon ,&nbsp;Sam Yeo ,&nbsp;Kinga Vojnits ,&nbsp;Rayhan Shah ,&nbsp;Sepideh Pakpour ,&nbsp;Simone Duarte","doi":"10.1016/j.archoralbio.2025.106405","DOIUrl":"10.1016/j.archoralbio.2025.106405","url":null,"abstract":"<div><h3>Objectives</h3><div>Charcoal-containing dentifrices are increasingly popular for their whitening claims, but data on antimicrobial effects are limited. This study compared the antibacterial efficacy of charcoal dentifrices versus non-charcoal dentifrices containing sodium fluoride (NaF), stannous fluoride (SnF₂), or sodium monofluorophosphate (NaMFP) against multi-species oral biofilms.</div></div><div><h3>Methods</h3><div>Biofilms of <em>Streptococcus mutans</em>, <em>S. gordonii</em>, and <em>S. sanguinis</em> were grown on hydroxyapatite discs and treated for 60 s with 6 dentifrice slurries (3 charcoal, 3 non-charcoal dentifrices) or controls (saline and 0.12 % chlorhexidine, CHX). Antibacterial effects were assessed by CFU/mL; (n = 9/group) and qPCR (n = 3/group). For fluoride-type analyses, charcoal and non-charcoal dentifrices were combined (CFU n = 18/type; qPCR n = 6/type). Percent reduction was compared across groups using one-way ANOVA with post-hoc tests.</div></div><div><h3>Results</h3><div>NaF dentifrices exhibited the greatest overall antibacterial activity (46.8 % reduction), followed by NaMFP (34.9 %), while SnF₂ showed minimal effect (≤ 5.7 %). Charcoal inclusion did not enhance efficacy and slightly reduced NaF activity. Species-specific responses varied: NaF eliminated <em>S. gordonii</em>, and significantly reduced <em>S. mutans</em> and <em>S. sanguinis</em>. Charcoal inclusion did not significantly alter species-level viability. qPCR supported CFU trends but showed limited between-group differences. Overall, fluoride type – not charcoal – primarily determined efficacy (NaF &gt; NaMFP &gt; SnF₂).</div></div><div><h3>Conclusions</h3><div>Fluoride type had a greater impact on antibacterial efficacy than charcoal. NaF was most effective, while SnF₂ least. Charcoal offered no benefit and may slightly diminish NaF performance. Fluoride choice is more critical than charcoal additives for caries prevention.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106405"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145187910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Combined bioinformatics and experimental validation identifies m1A-modified genes as potential diagnostic biomarkers in periodontitis and diabetes","authors":"Kai Wang ,&nbsp;Aiguo Wang ,&nbsp;Liewang Qiu ,&nbsp;Ling Xu ,&nbsp;Jingjing Wei","doi":"10.1016/j.archoralbio.2025.106428","DOIUrl":"10.1016/j.archoralbio.2025.106428","url":null,"abstract":"<div><h3>Objective</h3><div>To identify m1A-modified genes with diagnostic potential linking periodontitis and type 2 diabetes mellitus (T2DM) by integrating bioinformatics and experimental validation.</div></div><div><h3>Design</h3><div>Transcriptomic data for periodontitis and T2DM patients were integrated from the GEO database to analyze m1A-related gene expression. A diagnostic model was constructed using ridge and logistic regression. Gene function enrichment, immune infiltration, and protein-protein interaction analyses explored m1A regulatory mechanisms based on m1A scoring and patient clustering models. Gingival tissue samples were collected from periodontitis patients and healthy controls, and a streptozotocin-induced diabetic β-cell model was established. qRT-PCR was performed to validate candidate genes (<em>RRP8</em>, <em>ALKBH3</em>, <em>MAK16</em>, and <em>DDX18</em>). Statistical comparisons were conducted using the non-parametric Mann–Whitney U test.</div></div><div><h3>Results</h3><div>Several m1A-related genes were differentially expressed in both periodontitis and T2DM. <em>RRP8</em> and <em>ALKBH3</em> had high predictive value, with area under the curve (AUC) values of 0.80 (periodontitis) and 0.72 (T2DM). m1A scoring and patient clustering models effectively distinguished patient groups with distinct transcriptomic and immune profiles. Hub genes <em>MAK16</em> and <em>DDX18</em> showed consistent expression patterns and strong correlations with immune infiltration. qRT-PCR confirmed significant downregulation of <em>RRP8</em>, <em>ALKBH3</em>, <em>MAK16</em>, and <em>DDX18</em> in inflamed gingival tissues, and upregulation in the diabetic cell model (<em>p</em> &lt; 0.05), supporting the bioinformatics findings.</div></div><div><h3>Conclusions</h3><div>This integrative study identifies key m1A-modified genes potentially linking periodontitis and diabetes. The combination of bioinformatics analysis and experimental validation highlights their potential as diagnostic biomarkers and provides novel insights into shared molecular mechanisms of these comorbid conditions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106428"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered oral microbiome diversity in patients with oral candidiasis","authors":"Ji Rak Kim ,&nbsp;Jin-Seok Byun ,&nbsp;Jae-Kwang Jung ,&nbsp;Su-Hyung Hong ,&nbsp;Heon-Jin Lee","doi":"10.1016/j.archoralbio.2025.106430","DOIUrl":"10.1016/j.archoralbio.2025.106430","url":null,"abstract":"<div><h3>Objective</h3><div>Oral candidiasis is a common opportunistic infection caused by <em>Candida albicans</em>, particularly in individuals with local or systemic risk factors. This study aimed to investigate how antifungal therapy affects the composition of the oral bacterial microbiome.</div></div><div><h3>Design</h3><div>Unstimulated saliva samples were collected from ten patients diagnosed with acute pseudomembranous oral candidiasis before and after fluconazole treatment. Microbiome profiles were assessed using 16S rRNA gene sequencing. Quantitative PCR was performed to validate changes in specific bacterial species.</div></div><div><h3>Results</h3><div>Alpha diversity did not change significantly, whereas beta-diversity analyses indicated modest compositional shifts. Antifungal therapy was associated with an increase in <em>Streptococcus salivarius</em>, a commensal linked to mucosal health. The signal was confirmed by species-specific qPCR in paired samples.</div></div><div><h3>Conclusions</h3><div>Fluconazole treatment for oral candidiasis induces modest shifts in the oral bacterial community, particularly increasing the abundance of <em>S. salivarius</em>. These changes may reflect partial recovery of microbial homeostasis, supporting the role of microbiome monitoring and probiotic approaches in post-treatment care.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"180 ","pages":"Article 106430"},"PeriodicalIF":2.1,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145359026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}