Archives of oral biology最新文献

{"title":"Adsorption and release pattern of recombinant human bone morphogenic proatin 2 onto different bone grafts and its consequent osteoblasts` activation and neutrophils` priming","authors":"Adir Cohen ,&nbsp;Tom Avraham Verkauf ,&nbsp;Nardy Casap ,&nbsp;Tali Chackartchi ,&nbsp;David Polak","doi":"10.1016/j.archoralbio.2024.106123","DOIUrl":"10.1016/j.archoralbio.2024.106123","url":null,"abstract":"<div><h3>Objectives</h3><div>Controlled long-term delivery of recombinant human bone morphogenic proatin 2 (rhBMP2) eluted in a collagen scaffolds suffer from only a high initial burst release. The purpose of the current study was to investigate the long-term delivery of rhBMP2 when mixed with different bone grafts and its impact on osteoblastic activity and neutrophil priming.</div></div><div><h3>Methods</h3><div>rhBMP2 was separately mixed with xenograft, allograft, or alloplast and incubated for 30 days. Levels of BMP2 adsorption and their release were measured using immunofluorescence and ELISA respectively. The supernatants from the grafts were then incubated with either osteoblast (Saos-2 cells) or neutrophils (differentiated from HL60) for alkaline phosphatase and oxidative stress measurements respectively. Gene expression of osteoblast functionality and neutrophil priming were measured with qRT-PCR.</div></div><div><h3>Results</h3><div>rhBMP2 was adsorbed onto all tested grafts, with a superior effect of alloplast. The release of the rhBMP2 from all grafts was similar and sustained for 30 days with the lowest levels in the alloplast group. Activation of osteoblast was robust in the allograft and xenograft groups, concomitant with elevated osteocalcin expression. Neutrophil priming was greatest in the xenograft group, together with elevated expression of interleukin 1β.</div></div><div><h3>Conclusion</h3><div>rhBMP2 with bone graft material constitutes its sustained release over time. This, in turn, robust osteoblast and neutrophil activity.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"170 ","pages":"Article 106123"},"PeriodicalIF":2.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Porphyromonas gingivalis-induced autophagy exacerbates abnormal lung homeostasis: An in vivo and in vitro study","authors":"Qian Zhao ,&nbsp;Wenyue Li ,&nbsp;Wei Li,&nbsp;Hongjia Yang,&nbsp;Xueyuan Wang,&nbsp;Zhaoyue Ding,&nbsp;Zhiqiang Liu,&nbsp;Zuomin Wang","doi":"10.1016/j.archoralbio.2024.106122","DOIUrl":"10.1016/j.archoralbio.2024.106122","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to evaluate the effect of periodontal <em>Porphyromonas gingivalis (P. gingivalis)</em> infection on lung homeostasis and to explore the underlying mechanism.</div></div><div><h3>Designs</h3><div>In <em>in vivo</em> experiments, twelve mice were divided into two groups. The <em>P. gingivalis</em> infection group received <em>P. gingivalis</em> around the maxillary second molar, and the control group was left untreated. After 12 weeks, the histopathological changes of the lung tissue and the autophagy and apoptosis in the lung tissue cells were detected. In <em>in vitro</em> experiments, alveolar epithelial cell A549 was co cultured with <em>P. gingivalis</em> and treated with autophagy inhibitor chloroquine (CQ). Western blot was then used to detect autophagic markers LC3 and P62, and mRFP-GFP-LC3 was used to observe autophagic flux. Cell viability and apoptosis were also detected.</div></div><div><h3>Results</h3><div>For the <em>in vivo</em> experiments, pathological changes were observed in the lung tissue of the <em>P. gingivalis</em> infection group at 12 weeks, along with higher levels of autophagy and apoptosis in the lung tissue cells. For the <em>in vitro</em> experiments, infection of alveolar epithelial cells with <em>P. gingivalis</em> inhibited cell viability and promoted cell autophagy and apoptosis. Interestingly, we found that inhibiting <em>P. gingivalis-</em>activated autophagy significantly improved cell apoptosis and viability damage induced by <em>P. gingivalis</em>.</div></div><div><h3>Conclusion</h3><div>Periodontal <em>P. gingivalis</em> infection can cause pathological changes and abnormal homeostasis in lung tissue, and the up-regulation of autophagy induced by <em>P. gingivalis</em> may play a synergistic role in this process.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106122"},"PeriodicalIF":2.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acidic/abrasive challenges on simulated non-carious cervical lesions development and morphology","authors":"Giovanna C. Denucci ,&nbsp;Ian Towle ,&nbsp;Cecilia P. Turssi ,&nbsp;George J. Eckert ,&nbsp;Anderson T. Hara","doi":"10.1016/j.archoralbio.2024.106120","DOIUrl":"10.1016/j.archoralbio.2024.106120","url":null,"abstract":"<div><h3>Objectives</h3><div>This in vitro investigation assessed how frequency of erosive challenges and duration of toothbrushing abrasion influenced non-carious cervical lesions (NCCLs) development and morphology. Design: Experimental units were prepared using extracted human premolars assigned to four erosive-abrasive frequency protocols (n=16): F0. No acid exposure (negative control), F2.5 K. Acid exposure (1 % citric acid at natural pH) every 2500, F5K. 5000 and F15K. 15000 brushing-strokes. All groups were brushed for 55000 total brushing-strokes. Three-dimension images of the teeth were captured at baseline, after 15000, 35000 and 55000 brushing-strokes, using an intraoral scanner (TRIOS4, 3Shape). WearCompare software (Leeds Digital Dentistry) was used to analyze volumetric tooth loss (mm³) by superimposition followed by subtraction analysis. Lesion angle was measured (ImageJ, NIH) and morphology visually classified. Data were analyzed using ANOVA and Fisher’s Exact tests adopting two-sided 5 % significance level. Results: Tooth loss increased with brushing-strokes overall (p&lt;0.001) and for each erosive-abrasive protocol (p&lt;0.001). Acid exposure significantly increased tooth loss (p&lt;0.001), regardless of brushing interval (p&lt;0.001), however by 35000 strokes no tooth loss difference was observed among acid-exposed groups (p&gt;0.05). Control had significantly sharper mean lesion angle (59°) than all acid-exposed groups (∼145°) (p&lt;0.001), and significantly different lesion shape with 94 % wedge-shaped lesions versus 0 %, respectively (p&lt;0.001). In contrast to the control, acid exposure was associated to more striated lesions. Conclusions: Simulated NCCLs developed and progressed differently and more rapidly in the presence of acidic challenges, regardless of their frequency. Exposure to acid impacted the morphology of lesions.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106120"},"PeriodicalIF":2.2,"publicationDate":"2024-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142523806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of quercetin on mineralized dental tissues: A scoping review","authors":"Gabriel Pereira Nunes ,&nbsp;Renata de Oliveira Alves ,&nbsp;Matheus Henrique Faccioli Ragghianti ,&nbsp;Alexandre Henrique dos Reis-Prado ,&nbsp;Priscila Toninatto Alves de Toledo ,&nbsp;Tamires Passadori Martins ,&nbsp;Ana Paula Miranda Vieira ,&nbsp;Geórgia Rondó Peres ,&nbsp;Cristiane Duque","doi":"10.1016/j.archoralbio.2024.106119","DOIUrl":"10.1016/j.archoralbio.2024.106119","url":null,"abstract":"<div><h3>Objective</h3><div>This scoping review (SR) aimed to investigate the impact of quercetin on mineralized dental tissues intended to be used in preventive and restorative dentistry.</div></div><div><h3>Methods</h3><div>This SR was conducted following the PRISMA-ScR statement. A comprehensive search was performed across databases for articles published up to March 2024. Eligible studies included <em>in vitro</em> and <em>in situ</em> studies and evaluating the potential therapeutic effects of quercetin on dental enamel and dentin. Data were extracted, and synthesis of study findings was conducted.</div></div><div><h3>Results</h3><div>Out of the 2322 records screened, 22 studies were included in the review. Quercetin, in solution or into dental materials increased the bond strength to enamel and dentin. Additionally, quercetin also enhanced the bond strength of enamel after bleaching. Co-administration of quercetin with fluoride prevented erosive wear and inhibited the proteolytic activity in dentin more effectively than either agent alone. Hardness and modulus of elasticity was higher in dentin treated with quercetin compared to placebo. Reduction of nanoleakage at the composite-dentin interface was reduced in the presence of quercetin as a solution or incorporated into dental adhesives.</div></div><div><h3>Conclusions</h3><div>Quercetin exhibits promising therapeutic effects on mineralized dental tissues, including remineralization and enhancement of bond strength. It shows potential as a multifunctional agent for improving the longevity and effectiveness of dental biomaterials, as well as in preventing erosion and dental caries. However, as these conclusions are largely drawn from lab-based (<em>in vitro</em>) studies, further research, including clinical trials, is needed to fully explore its therapeutic potential and applications in dentistry.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106119"},"PeriodicalIF":2.2,"publicationDate":"2024-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allyl isothiocyanate suppressed periodontal tissue destruction in mice via bacteriostatic and anti-inflammatory activities against Porphyromonas gingivalis","authors":"Yukako Minato,&nbsp;Yukari Aoki-Nonaka,&nbsp;Hnin Yu Lwin,&nbsp;Daiki Ando,&nbsp;Yuko Warita,&nbsp;Aoi Matsugishi-Nasu,&nbsp;Takumi Hiyoshi,&nbsp;Naoki Takahashi,&nbsp;Koichi Tabeta","doi":"10.1016/j.archoralbio.2024.106118","DOIUrl":"10.1016/j.archoralbio.2024.106118","url":null,"abstract":"<div><h3>Objectives</h3><div>Allyl isothiocyanate (AITC) is a phytochemical that is abundantly present in cruciferous vegetables, such as wasabi and mustard. Among its pharmacological properties, it demonstrates anticancer, antifungal, and anti-inflammatory activities. This study aimed to investigate the functions of AITC against periodontopathic bacteria and its effects on a mouse model of periodontitis.</div></div><div><h3>Design</h3><div>The antimicrobial and antibiofilm functions of AITC were assessed against <em>Porphyromonas gingivalis, Fusobacterium nucleatum,</em> and <em>Streptococcus mitis</em>. To clarify its anti-inflammatory effects, macrophage-like cells from THP-1 were stimulated with <em>P. gingivalis</em> lipopolysaccharide (LPS), and the release of inflammatory cytokines was analyzed by ELISA. Experimental periodontitis was induced in 9-week-old mice by ligation and oral infection of <em>P. gingivalis</em>, and AITC was injected into the gingiva once daily for 8 days. Alveolar bone resorption was evaluated by measuring the exposed root area. Gene expressions in the periodontal tissue were analyzed via qPCR.</div></div><div><h3>Results</h3><div>AITC exerted weak bacteriostatic effects against <em>P. gingivalis,</em> inhibiting biofilm formation. AITC also impeded the production of interleukin-6 and tumor necrosis factor-α induced by <em>P. gingivalis</em> LPS. Additionally, transient receptor potential ankyrin 1(TRPA1) channel agonist inhibited the anti-inflammatory effects of AITC. In vivo, AITC inhibited alveolar bone destruction and decreased the gene transcription of <em>Il6</em> in the periodontal tissue.</div></div><div><h3>Conclusion</h3><div>AITC exerted weak bacteriostatic and anti-inflammatory effects against <em>P. gingivalis,</em> reducing alveolar bone destruction and suppressing the inflammatory response in experimental periodontitis. Therefore, AITC may serve as a valuable adjunct in controlling periodontal disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106118"},"PeriodicalIF":2.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142560874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How malocclusion interferes with tissue inhibitor of metalloproteinase-1 expression and morphology of the articular cartilage of the mandible in female rats","authors":"Carolina Brioschi Mathias ,&nbsp;Rebeca Ferreira Badaró ,&nbsp;Willian Grassi Bautz ,&nbsp;Leticia Nogueira da Gama-de-Souza","doi":"10.1016/j.archoralbio.2024.106117","DOIUrl":"10.1016/j.archoralbio.2024.106117","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this study was to investigate morphological alterations and tissue inhibitor of metalloproteinase-1 expression in the articular cartilage of the mandible under conditions of experimentally induced malocclusion.</div></div><div><h3>Design</h3><div>Twenty-four 8-week-old female Wistar rats were used and divided into control and experimental groups with two different treatment periods (2 and 4 weeks). Sagittal malocclusions were orthodontically created, causing mesial movement of the first molars and distalization of the third molars unilaterally and on opposite sides of the arches. Sagittal sections of the articular cartilage of the mandible were subjected to hematoxylin and eosin and immunohistochemistry for tissue inhibitor of metalloproteinase-1. Chi-square and Mann<img>Whitney U tests were applied.</div></div><div><h3>Results</h3><div>Animals treated for 2 and 4 weeks showed morphological alterations in articular cartilage of the mandible. The main findings were thickening of the posterior third, layer derangement, osteoclast activity and osteophyte formation. Among the cellular aspects, the presence of chondrocytes with condensed nuclei and cytoplasm reduction were observed. The enzyme in control animals was observed only in the mature layer. Treated animals showed immunopositive cells in the proliferative and mature layers, and in the 2-week treated group, the posterior third of the cartilage had more immunolabeled cells than control (<em>P=0.0291</em>).</div></div><div><h3>Conclusions</h3><div>The occlusal disorder caused morphological changes in articular cartilage of the mandible, probably due to the attempt to adapt to the new condition. Tissue inhibitor of metalloproteinase-1 expression may play a role as an initial modulator in the biological events observed in articular cartilage of the mandible.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106117"},"PeriodicalIF":2.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The important role of the Wnt/β-catenin signaling pathway in small molecules mediated gingival mesenchymal stem cells transdifferentiate into neuron-like cells","authors":"Qiuying Liang ,&nbsp;Chuhan Zhang ,&nbsp;Peiyi Lv ,&nbsp;Yongmao Huang ,&nbsp;Hang Zhao ,&nbsp;Shan Jiang ,&nbsp;Wenan Xu","doi":"10.1016/j.archoralbio.2024.106115","DOIUrl":"10.1016/j.archoralbio.2024.106115","url":null,"abstract":"<div><h3>Objective</h3><div>Given their neural crest origin, gingival mesenchymal stem cells (GMSCs) possess high neurogenic potential, which makes them suitable for cell replacement therapy against neurodegenerative diseases. This study investigated whether GMSCs can be transdifferentiated into neurons <em>in vitro</em> using a protocol involving small molecules VCRFY (VPA, CHIR99021, Repsox, Forskolin, and Y-27632). The regulatory mechanisms of key signaling pathways were also investigated.</div></div><div><h3>Methods</h3><div>Neuronal induction of GMSCs was conducted using a small molecules-based protocol over 7 days, which included the evaluation of cell morphology, proliferation, expressions of neurogenic markers, and intracellular calcium oscillation. The activation of canonical the Wnt signaling pathway was assessed by examining the protein content and subcellular localization of β-catenin.</div></div><div><h3>Results</h3><div>Small molecules-treated GMSCs displayed neuronal morphology and increased expression of neurogenic markers, including class III beta-tubulin (TUJ1), neuron-specific enolase (NSE), microtube-associated protein 2 (MAP2), and neurofilament medium (NFM), verified through RT-qPCR, western blotting, and immunocytochemistry. Based on the results of Fluo-4 AM calcium flux assay, small molecules-treated GMSCs exhibited enhanced electrophysiological activity. GMSC proliferation halted after 2 days of treatment. Among the small molecules, CHIR99021 exhibited the highest neuronal induction efficiency. Furthermore, activation of the Wnt/β-catenin signaling pathway augmented neuronal differentiation.</div></div><div><h3>Conclusions</h3><div>Small molecule-based cellular reprogramming can efficiently generate neurons from GMSCs, with Wnt/β-catenin signaling to play a critical role in neuronal induction.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106115"},"PeriodicalIF":2.2,"publicationDate":"2024-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of salivary melatonin and MMP-9 levels in periodontal diseases","authors":"Ali Batuhan Bayırlı ,&nbsp;Ceyda Gürhan ,&nbsp;Ercan Saruhan","doi":"10.1016/j.archoralbio.2024.106116","DOIUrl":"10.1016/j.archoralbio.2024.106116","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to evaluate salivary matrix metalloproteinase-9 (MMP-9) and melatonin levels in individuals with periodontal health, gingivitis, and periodontitis.</div></div><div><h3>Design</h3><div>A total of 170 participants were enrolled in this study. They included 57 periodontally healthy individuals, 58 gingivitis patients, and 55 periodontitis patients. Saliva samples were collected by passive drool technique. The levels of MMP-9 and melatonin in saliva were measured biochemically using the ELISA method.</div></div><div><h3>Results</h3><div>Salivary MMP-9 levels in the periodontitis group were significantly higher than those in the gingivitis and periodontally healthy groups, while salivary melatonin levels were significantly lower (<em>p</em>&lt;0.001). A positive correlation was observed between clinical periodontal parameters and salivary MMP-9 levels, while salivary melatonin levels were negatively correlated (<em>p</em>&lt;0.001). A negative correlation was also observed between salivary MMP-9 levels and salivary melatonin levels (<em>p</em>&lt;0.001).</div></div><div><h3>Conclusion</h3><div>This study shows that the level of melatonin in saliva is associated with periodontal disease and with the level of MMP-9 in saliva, which plays a role in this disease.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106116"},"PeriodicalIF":2.2,"publicationDate":"2024-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mimicking and in vitro validating chronic inflammation in human gingival fibroblasts","authors":"Anne Eriksson Agger ,&nbsp;Athina Samara ,&nbsp;Tianxiang Geng ,&nbsp;Ole Kristoffer Olstad ,&nbsp;Janne Elin Reseland","doi":"10.1016/j.archoralbio.2024.106113","DOIUrl":"10.1016/j.archoralbio.2024.106113","url":null,"abstract":"<div><h3>Objective</h3><div>The aim of this study was to identify and validate in vitro conditions that may mimic the translational, cytokine and chemokine profiles observed in human inflamed gingiva in vivo.</div></div><div><h3>Design</h3><div>Primary human gingiva fibroblast cells (HFIB-G) were cultured under serum starvation conditions (0 – 10 %), supplemented with increasing lipopolysaccharide (LPS) concentrations (0.1, 1, or 10 µg/ml) from two bacterial strains <em>E. coli</em> and <em>P. gingivalis</em> and 0.1, 1, or 10 ng/ml recombinant interleukin 1β (IL-1β), alone or in combinations. The levels of cytokines/chemokines were measured in the cell culture medium by Luminex, and gene expression was quantified by Affymetrix microarrays at 24, 48 and 72 h.</div></div><div><h3>Results</h3><div>Inflammation markers were not elevated after stimulation with <em>P. gingivalis</em> LPS, while <em>E. coli</em> LPS and IL-1β individually increased the secretion of interleukin 6 (IL-6) and monocyte chemoattractant protein-1 (MCP-1) to the cell culture medium. IL-1β administration also increased the secretion of several factors, including tumor necrosis factor (TNFα). However, the combination of 1 µg/ml <em>E. coli</em> LPS, 1 ng/ml IL-1β and serum starvation led to increased secretion of IL-6, TNFα, in addition to other factors found in inflamed tissue. Gene expression analyses revealed that this combination not only enhanced the expression interleukins/chemokines genes but also T helper cell signaling and matrix metalloproteinases.</div></div><div><h3>Conclusion</h3><div>Serum reduction in cell culture medium together with the administration of <em>E. coli</em> LPS and IL-1β resulted in gene expression and secreted cytokine/chemokine profiles similar to that found in vivo during chronic inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106113"},"PeriodicalIF":2.2,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of botulinum toxin type A on tooth movement and bone remodeling in male Wistar rats","authors":"Joana Estephany Gordillo Yépez ,&nbsp;Renata Machado Marangon ,&nbsp;Aline Cristina Batista Rodrigues Johann ,&nbsp;Bruno Massa de Viveiros ,&nbsp;Patricia Kern Di Scala Andreis ,&nbsp;Luana Vosgerau ,&nbsp;Sara Moreira Leal Salvação ,&nbsp;Orlando Motohiro Tanaka ,&nbsp;Odilon Guariza-Filho ,&nbsp;Sergio Aparecido Ignácio ,&nbsp;Elisa Souza Camargo","doi":"10.1016/j.archoralbio.2024.106105","DOIUrl":"10.1016/j.archoralbio.2024.106105","url":null,"abstract":"<div><h3>Objective</h3><div>We evaluated whether the use of botulinum neurotoxin type A (BTX-A) in masticatory muscles influences tooth movement and bone remodeling.</div></div><div><h3>Design</h3><div>Seventy-seven male Wistar rats were allocated to the groups: S - Saline (n=20); SM - Saline with movement (n=20); BT - Botulinum toxin (n=18); BTM - Botulinum toxin with movement (n=19). On day 1, 0.02 mL of sterile 0.9 % saline was administered to groups S and SM and BTX-A (1 U in 0.02 mL of saline) to groups BT and BTM, in the masseter and temporal muscles laterally. On day 30, a nickel titanium spring was installed to move the first maxillary molar and euthanasia was performed on days 32 and 51. Tooth displacement, maxillary and mandibular bone volumes, collagen neoformation, bone and root resorptions, and masseter morphometry were assessed. Statistical analysis was conducted (<em>p</em> &lt; 0.05).</div></div><div><h3>Results</h3><div>A higher percentage of type I collagen was observed in the BT than in the S group on day 51 and lower mass, length, and diameter of the masseter fibers in BT and BTM (<em>p</em> &lt; 0.05). Tooth displacement, bone volume, bone and root resorptions, hyaline area, and masseter height showed no difference among groups with and without BTX-A, regardless of tooth movement (<em>p</em> &gt; 0.05).</div></div><div><h3>Conclusions</h3><div>BTX-A did not interfere with tooth displacement, bone volume, and dental and periodontal tissues related to tooth movement in rats; it increased mature collagen in animals without tooth movement; and it caused a decrease in the mass, length, and diameter of the masseter fibers.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"169 ","pages":"Article 106105"},"PeriodicalIF":2.2,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}