Archives of oral biology最新文献

{"title":"DPM1 expression as a potential prognostic tumor marker in oral squamous cell carcinoma","authors":"Hongyu Liu ,&nbsp;Buling Wu","doi":"10.1016/j.archoralbio.2025.106432","DOIUrl":"10.1016/j.archoralbio.2025.106432","url":null,"abstract":"<div><h3>Objective</h3><div>This study investigates how dolichol phosphate mannose synthase (DPMS) subunits DPM1/2/3 affect oral squamous cell carcinoma (OSCC) prognosis and their associations with OSCC.</div></div><div><h3>Design</h3><div>To evaluate the connections between DPMS subunits DPM1/2/3 and OSCC, we utilized a wide array of databases and analytical resources, including The Cancer Genome Atlas (TCGA), Metascape, and MethSurv. For initial verification, we applied real-time quantitative polymerase chain reaction (RT-qPCR) to OSCC cell lines and tissues.</div></div><div><h3>Results</h3><div>DPM1 and DPM2 expression was higher in OSCC tissues than normal ones. Univariate and multivariate COX analyses showed high DPM1 expression independently correlated with poorer OSCC overall survival. Gene ontology (GO) analysis via Metascape revealed DPM1/2/3 and co-expressed genes enriched in cell cycle, nucleic acid metabolism, and translation. Gene set enrichment analysis (GSEA) linked them to pathways like complement activation, BCR signaling, and immune systems. Besides, high DPMS expression was tied to a more unfavorable prognosis in OSCC patients, and DPM1/2/3 was associated with the infiltration of tumor immune cells in OSCC patients. We found that methylation levels might be related to the prognosis of OSCC patients. By means of RT-qPCR, we ascertained the differential expression levels of DPM1 in human normal oral keratinocytes (HOK) and human tongue squamous cell carcinoma cells (Cal27, SCC9), cancer and paracancerous tissues of OSCC.</div></div><div><h3>Conclusion</h3><div>Our findings imply that DPM1, a subunit of DPMS, can serve as a potential molecular indicator for poor prognosis in OSCC and may act as a novel target in OSCC treatment strategies.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106432"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ganoderic acid A attenuates Porphyromonas gingivalis-induced adhesion molecule expression in gingival fibroblasts and tissues via inhibition of NLRP6 inflammasome activation","authors":"Yuanbo Wang ,&nbsp;Jianru Liu ,&nbsp;Xiangying Ouyang ,&nbsp;Wenyi Liu ,&nbsp;Peiying Lyu ,&nbsp;Shengnan Zhang ,&nbsp;Jing Guo","doi":"10.1016/j.archoralbio.2025.106429","DOIUrl":"10.1016/j.archoralbio.2025.106429","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to elucidate the regulatory effects and mechanisms of Ganoderic acid A (GAA) on <em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>)-induced expression of adhesion molecules in human gingival fibroblasts (hGFs) and periodontal tissues, focusing on NLRP6 inflammasome modulation.</div></div><div><h3>Design</h3><div>HGFs were stimulated with <em>P. gingivalis</em> strain W83. The expression levels of NLRP6, caspase-1, IL-1β,IL-18 and adhesion molecules ICAM-1 &amp; VCAM-1 were analyzed by RT-PCR, Western blotting and ELISA, with or without GAA pretreatment. To further explore the regulatory role of GAA on the NLRP6 inflammasome, NLRP6 overexpression assays were performed in hGFs, followed by assessment of ICAM-1 and VCAM-1 expressions. Additionally, a ligature-induced periodontitis mice model was established to evaluate the effects of GAA on NLRP6 expression and inflammatory cell infiltration in periodontal tissues.</div></div><div><h3>Results</h3><div>GAA exhibited no cytotoxicity toward hGFs at low concentrations (within 16 μM). GAA pretreatment significantly inhibited <em>P. gingivalis</em>-induced activation of the NLRP6 inflammasome. Beyond IL-1β and IL-18, it also reduced ICAM-1 and VCAM-1 expression at both mRNA and protein levels. Overexpression of NLRP6 increased the expression of NLRP6 inflammasome components and adhesion molecules, whereas GAA treatment effectively suppressed these elevations. Furthermore, GAA administration markedly downregulated NLRP6 expression and attenuated inflammatory cell infiltration and periodontal inflammation in the mice periodontitis model.</div></div><div><h3>Conclusions</h3><div>GAA attenuates <em>P. gingivalis</em>-induced expression of adhesion molecules by inhibiting NLRP6 inflammasome activation in gingival fibroblasts and periodontal tissues, thereby alleviating inflammatory cell infiltration. These findings suggest the potential of GAA as a therapeutic agent for the management of periodontal inflammation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106429"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145319002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel missense mutations in the tumor necrosis factor domain of Ectodysplasin-A cause non-syndromic tooth agenesis in two Chinese families","authors":"Chengcan Yang ,&nbsp;Nuo Xu ,&nbsp;Xiaona Song ,&nbsp;Kai Yang ,&nbsp;Qian Gao","doi":"10.1016/j.archoralbio.2025.106441","DOIUrl":"10.1016/j.archoralbio.2025.106441","url":null,"abstract":"<div><h3>Objective</h3><div>Mutations of the Ectodysplasin-A (<em>EDA</em>) gene are generally associated with syndromic tooth agenesis, yet their influence on non-syndromic tooth agenesis (NSTA) has not been thoroughly elucidated. This study aimed to investigate the genetic basis of NSTA and to identify pathogenic EDA mutations in two Chinese families using whole-exome sequencing, and explore the underlying mechanisms involved.</div></div><div><h3>Design</h3><div>Two unrelated individuals with NSTA and their families were enrolled in this study, and genomic DNA was extracted from peripheral blood samples. Whole-exome sequencing was performed, followed by variants detection and filtering to identify pathogenic genes and mutations. The identified genetic mutations were further validated by Sanger sequencing and confirmed to be absent in 300 unrelated healthy individuals. Conservation analysis, three-dimensional (3D) modeling, and electrostatic potential calculations were conducted accordingly.</div></div><div><h3>Results</h3><div>We identified a novel mutation c.827 G&gt;T (p.Arg276Leu) and a previously reported mutation c.1069 C&gt;T (p.Arg357Trp) in the <em>EDA</em> gene, with the latter showing previously unreported phenotypic characteristics. Both mutations are located in the tumor necrosis factor (TNF) domain of EDA, which is crucial for binding with ectodysplasin A receptor (EDAR). Conservation analysis indicated that these mutations are evolutionarily conserved across various species. In silico predictions and 3D modeling analyses suggested that these mutations may be pathogenic, potentially due to alterations in the hydrogen bonding and electrostatic potential, thereby affecting EDA-EDAR interaction.</div></div><div><h3>Conclusions</h3><div>Our findings broaden the mutation spectrum of <em>EDA</em> gene associated with NSTA by identifying a novel mutation and provide a basis for future genetic counseling.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106441"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145446809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Traditional Chinese medicine Pudilan synergizes with smc gene to impair cell growth and exopolysaccharides synthesis of Streptococcus mutans","authors":"Hongyu Zhang,&nbsp;Yiting Cheng,&nbsp;Yalan Deng,&nbsp;Yingming Yang,&nbsp;Lei Lei,&nbsp;Tao Hu","doi":"10.1016/j.archoralbio.2025.106439","DOIUrl":"10.1016/j.archoralbio.2025.106439","url":null,"abstract":"<div><h3>Objective</h3><div>This study examines the impact of Pudilan mouthwash in conjunction with the <em>smc</em> gene of <em>Streptococcus mutans</em> (<em>S. mutans</em>) on biofilm formation.</div></div><div><h3>Design</h3><div>We constructed the <em>smc</em> gene null mutant strains of <em>S. mutans</em> (Δsmc) via homologous recombination, and overexpression strains (smc^OE) and complementary strains (smc^Comp) through chemical transformation of <em>smc</em> recombinant plasmid. We then tested Pudilan's MIC and MBC on UA159 parent strains and <em>smc</em> mutants. Growth curves were used to analyze growth rates. Bacterial viability was assessed with Live/Dead Staining. Biofilm formation and structure were examined using crystal violet staining, confocal laser scanning microscopy, and scanning electron microscopy. Genes involved in exopolysaccharides (EPS) metabolism were quantified by qRT-PCR. Network pharmacology built a compound-target network, and molecular docking studied interactions between Pudilan's components and <em>S. mutans</em> proteins.</div></div><div><h3>Results</h3><div>The combination of Pudilan and knockout of <em>smc</em> gene exhibited a synergistic antibacterial effect on <em>S. mutans</em>. Results indicated that this pairing effectively inhibited the growth and viability of <em>S. mutans,</em> reduced biofilm biomass, and rearranged the structure of 8-hour and 24-hour biofilms. Additionally, Pudilan treatment downregulated gene expression related to water-soluble glucan in Δsmc. Compounds like (S)-cheilanthifoline in Pudilan may significantly influence dental plaque development by modulating GtfD activity.</div></div><div><h3>Conclusions</h3><div>This finding demonstrates that Pudilan enhances the inhibitory effect caused by <em>smc</em> deletion on <em>S. mutans</em> growth and viability, reduces biofilm EPS, and downregulates the expression of genes involved in water-soluble glucan synthesis.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106439"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145402963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gingival crevicular fluid levels of soluble triggering receptor expressed on myeloid cells 1 in chronic periodontitis: A systematic review and meta-analysis","authors":"Jingsong Kong,&nbsp;Weiqiu Jin,&nbsp;Min Wang,&nbsp;Fuhua Yan ,&nbsp;Yanfen Li","doi":"10.1016/j.archoralbio.2025.106440","DOIUrl":"10.1016/j.archoralbio.2025.106440","url":null,"abstract":"<div><h3>Objectives</h3><div>To compare gingival crevicular fluid (GCF) levels of soluble Triggering Receptor Expressed on Myeloid cells 1 (sTREM-1) between patients with chronic periodontitis (CP) and those with healthy periodontal (HP) status, and evaluate its predictive value for periodontal disease progression.</div></div><div><h3>Design</h3><div>Systematic review and meta-analysis.</div></div><div><h3>Data sources</h3><div>PubMed, MEDLINE, Cochrane, Web of Science, EMBASE, CNKI, and Wanfang were searched through December 2024. Manual searches were performed.</div></div><div><h3>Eligibility criteria</h3><div>Only studies reporting GCF sTREM-1 CP patients and HP controls were included. Clinical trials, cross-sectional, and observational studies were included.</div></div><div><h3>Data extraction and synthesis</h3><div>Two independent reviewers extracted data and assessed the quality. Sensitivity analyses ensured result stability. Quality assessment was performed according to the Newcastle-Ottawa Scale. Random-effects model calculated standardized mean differences (SMD)with 95 % CI via Review Manager software. Publication bias was evaluated using funnel plots and Egger's tests. The certainty of the evidence was assessed using the Grading of Recommendations, Assessment, Development and Evaluations (GRADE) approach.</div></div><div><h3>Results</h3><div>Among 128 screened studies, 9 articles met eligibility criteria of the systematic review, and 6 articles (147 CP patients, 116 HP controls) were included in the meta-analysis. GCF sTREM-1 levels were higher in the CP group than HP group (SMD = 0.70; <span><math><mi>Z</mi></math></span> = 3.25; <span><math><mi>P</mi></math></span>&lt;0.001, 95 % CI).</div></div><div><h3>Conclusions</h3><div>This study found higher GCF sTREM-1 levels in CP patients compared to HP controls, suggesting potential as a diagnostic marker. However, small sample sizes and methodological variability limit the findings.</div></div><div><h3>PROSPERO registration number</h3><div>CRD42022326022</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106440"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145402862","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Patterns of circulating autoantibodies against desmoglein-1 and desmoglein-3 in the progression of oral lichen planus: A scoping review","authors":"Andari Sarasati ,&nbsp;Kadek Gede Putra Wibawa ,&nbsp;Meircurius Dwi Condro Surboyo ,&nbsp;Rene Luis Panico ,&nbsp;Arvind Babu Rajendra Santosh ,&nbsp;Adhistya Viany ,&nbsp;Dewi Agustina ,&nbsp;Diah Savitri Ernawati","doi":"10.1016/j.archoralbio.2025.106431","DOIUrl":"10.1016/j.archoralbio.2025.106431","url":null,"abstract":"<div><h3>Objective</h3><div>The potential role of autoantibodies targeting desmosomal proteins, particularly desmoglein 1 (Dsg-1) and desmoglein 3 (Dsg-3), in modulating disease severity and epithelial integrity in oral lichen planus (OLP) remains a subject of growing interest. Distinct patterns of anti-Dsg-1 and anti-Dsg-3 autoantibodies across OLP subtypes may correlate with disease progression. This scoping review investigates the expression patterns of circulating anti-Dsg-1 and anti-Dsg-3 autoantibodies in reticular, erosive, and ulcerative forms of OLP to elucidate their potential roles in epithelial disruption and disease advancement.</div></div><div><h3>Design</h3><div>Following PRISMA guidelines, a systematic search of four electronic databases was conducted. Eligible studies included case reports, case series, case-control and cross-sectional studies that reported serum levels of anti-Dsg-1 and anti-Dsg-3 autoantibodies in OLP patients.</div></div><div><h3>Results</h3><div>Reticular-type OLP showed variable anti-Dsg-1 and anti-Dsg-3 autoantibody levels, with some patients testing positive while others were negative, indicating an inconsistent involvement of desmosomal autoimmunity in this milder form of the disease.</div><div>In erosive-type OLP, the presence of both autoantibodies indicated deeper epithelial damage and an active autoimmune response. Interestingly, ulcerative-type OLP consistently tested negative for both antibodies despite extensive epithelial breakdown, implying a potential shift from desmosomal autoimmunity to chronic inflammation–driven tissue destruction that could compromise human health.</div></div><div><h3>Conclusion</h3><div>These findings suggest a potential continuum in which reticular OLP may progress to erosive OLP, with Dsg-3 autoantibodies as a possible early marker of worsening pathology. Ulcerative OLP appears to represent a late-stage, inflammation-driven phenotype with minimal autoantibody involvement. Given the limited and heterogeneous data, these observations are preliminary and should be interpreted cautiously.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106431"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145357183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial extracellular vesicles from Porphyromonas gingivalis – proteomic profile and influence on oral fibroblasts","authors":"Helene Rygvold Haugsten ,&nbsp;Anne Karin Kristoffersen ,&nbsp;Morten Enersen ,&nbsp;Bernd Thiede ,&nbsp;Tine M. Søland ,&nbsp;Trude M. Haug ,&nbsp;Hilde Kanli Galtung","doi":"10.1016/j.archoralbio.2025.106424","DOIUrl":"10.1016/j.archoralbio.2025.106424","url":null,"abstract":"<div><h3>Objectives</h3><div>In this study, we aimed to explore the protein content of bacterial extracellular vesicles (bEVs) from three strains of <em>Porphyromonas gingivalis</em>, an anaerobic gram-negative bacterium closely associated with periodontitis. Additionally, we aimed to investigate the effects of bEV uptake on human oral fibroblasts, to better understand the role of these vesicles in the pathogenesis of periodontitis.</div></div><div><h3>Design</h3><div>Liquid chromatography-mass spectrometry was performed on bEVs from three strains of <em>P. gingivalis</em> with different virulence: ATCC 33277, A7A1–28, and W83. Three key proteins associated with virulence were validated using dot blot. Proliferation and viability of human oral fibroblasts after incubation with bEVs were assessed at different timepoints and different concentrations of bEVs.</div></div><div><h3>Results</h3><div>A total of 307 proteins were identified in the bEVs from the three strains of <em>P. gingivalis</em>. Among those were several relevant for virulence, for example gingipains, fimbriae, and peptidyl-arginine deiminase (PPAD). When oral fibroblasts were exposed to bEVs from A7A1–28 or W83, proliferation was significantly affected after 12 and 24 h at one bEV concentration. Furthermore, viability was significantly affected by bEVs from W83 at several timepoints and bEV concentrations. bEVs from ATCC 33277 did not induce any significant changes.</div></div><div><h3>Conclusion</h3><div>Several proteins associated with virulence were detected in bEVs from all three strains of <em>P. gingivalis,</em> with varying abundance. Uptake of these bEVs can influence human cells, as exemplified by changes in viability and proliferation.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106424"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of nutrient canals in relation to jaw cysts, trabecular bone structure and dental regions: A CBCT study","authors":"Yasemin Merve Yılmaz ,&nbsp;Defne Yalçın","doi":"10.1016/j.archoralbio.2025.106433","DOIUrl":"10.1016/j.archoralbio.2025.106433","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the relationship of nutrient canals with jaw cysts, trabecular bone density, and dental regions using cone-beam computed tomography (CBCT).</div></div><div><h3>Design</h3><div>This retrospective study analyzed 120 CBCT images from 60 patients with unilateral jaw cysts. Nutrient canals were identified and localized on both the cyst-affected side and contralateral, unaffected side of the jaw for each patient. Alveolar trabecular structure was assessed by comparing Hounsfield Unit (HU) values between affected and unaffected sides. Statistical analyses included paired t-tests, Fisher’s exact test, and intra-observer reliability assessment.</div></div><div><h3>Results</h3><div>Nutrient canals were significantly more prevalent in cyst-affected jaw regions. Their presence was associated with areas exhibiting pericystic trabecular sclerosis, as reflected by elevated HU values. While nutrient canals were commonly observed in the anterior regions of both healthy and affected jaws, their presence in premolar and molar regions was restricted to cyst-affected areas.</div></div><div><h3>Conclusions</h3><div>Nutrient canals, typically found in the anterior jaws, appear to develop in posterior regions in association with jaw cysts, particularly in the presence of trabecular sclerosis. Their identification on CBCT scans is important for treatment planning and for minimizing potential surgical complications.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106433"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145360718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization and evaluation of antibacterial extracts from Acacia nilotica, Psidium guajava, Syzygium aromaticum, and Salvadora persica to inhibit dental caries","authors":"Chamundeswari Kandasamy ,&nbsp;Arunadevi Baladhandapani ,&nbsp;Renuka Devi Ponnuswamy ,&nbsp;Hemananthan Eswaran ,&nbsp;Vinodhini Karthikeyan ,&nbsp;Saranya Rajendran","doi":"10.1016/j.archoralbio.2025.106426","DOIUrl":"10.1016/j.archoralbio.2025.106426","url":null,"abstract":"<div><h3>Objective</h3><div>Dental caries is a significant global issue, which affects approximately 44 % of the global population. The objective of this research is to extract crude phytochemicals from <em>Psidium guajava</em>, <em>Acacia nilotica</em>, <em>Syzygium aromaticum</em>, and <em>Salvadora persica</em> and also optimizing using RSM. Therefore, this aims to maximize the antibacterial potential of the extracts at effective inhibition of dental caries</div></div><div><h3>Method</h3><div>The crude extracts from the plants samples, with varying extraction parameters, such as ethanol concentrations, temperatures, and solid-solvent ratios was done using Soxhlet method and antibacterial activity was determined through well and disc diffusion methods along with optimization was performed by Response Surface Methodology (RSM).</div></div><div><h3>Results</h3><div>The results depict that <em>Psidium guajava</em> exhibited the strongest antibacterial activity against <em>S. mutans</em> and <em>Lactobacillus</em>, with inhibition zones of 36 mm &amp; 20 mm and 32 mm &amp; 24 mm for both well and disc diffusion. Similarly, <em>Syzygium aromaticum</em> also shows slight stronger inhibition zones, <em>Acacia nilotica</em> exhibited moderate activity and in contrast, <em>Salvadora persica</em> showed limited activity. RSM optimization was validated and with well fitted quadratic models R² values ranging from 0.69 to 0.79. Predicted and experimental values are in close agreement between with differences below 0.5 %.</div></div><div><h3>Conclusion</h3><div>These findings suggest that <em>P. guajava</em>, <em>S. aromaticum</em> and <em>A. nilotica</em> have strong potential as natural antibacterial agents in oral care formulations.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106426"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145575031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Accuracy of dental age estimation methods in children with chromosomal syndromes: A systematic review and network meta-analysis","authors":"Ilham Wan Mokhtar ,&nbsp;Norsaima Ismail ,&nbsp;Mohd Yusmiaidil Putera Mohd Yusof","doi":"10.1016/j.archoralbio.2025.106445","DOIUrl":"10.1016/j.archoralbio.2025.106445","url":null,"abstract":"<div><h3>Objective</h3><div>The study aims to systematically examine the accuracy of the application of different dental age estimation (DAE) methods on children with chromosomal syndromes and evaluate its performance based on various populations and regions.</div></div><div><h3>Design</h3><div>A systematic search of the literature applying the PRISMA-NMA-compliant method was conducted using the databases PubMed, Web of Science, Science Direct, Google Scholar, and Scopus between 1981 and 2020; that estimate dental development of children with chromosomal syndrome based on populations, intervention, comparisons, and outcomes (PICO) search strategy identified. The literature quality was assessed using QUADAS-2. A network meta-analysis was performed to compare the effects of different chromosomal syndromes on the accuracy of estimated dental age (DA). Mean differences were calculated for difference of DA to chronological age (CA) by using the random effects model.</div></div><div><h3>Results</h3><div>From 60 titles retrieved utilising a standardized search strategy, 45 titles met the qualitative analysis criteria, and 28 titles qualified for the quantitative analysis requirements. Twenty-four comparative studies and twenty-one non-comparative studies suggested five DAE methods within this population used in combination or alone, namely Demirjian, Willems, Nolla, Haavikkoo, and London Atlas. Down’s syndrome is the highest contributing literature relating to DAE and chromosomal syndrome.</div></div><div><h3>Conclusions</h3><div>Underestimations were seen in Amelogenesis Imperfecta and Osteogenesis Imperfecta for Demirjian method. Other methods were lacking primary studies to make a reliable inference. This suggests that no single dental age estimation (DAE) method works consistently across diverse syndromes, emphasizing the importance of personalized approaches in clinical and forensic settings. Publication bias was minor, indicating solid findings.</div></div>","PeriodicalId":8288,"journal":{"name":"Archives of oral biology","volume":"181 ","pages":"Article 106445"},"PeriodicalIF":2.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145526060","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}