Archives of Microbiology最新文献

{"title":"N-acyl homoserine lactone quorum-sensing inhibitor acts as an antibiotic adjuvant to increase the susceptibility of Pseudomonas aeruginosa against β-lactam antibiotics","authors":"Juanyan Liu,&nbsp;Zhenyu Wei,&nbsp;Xinran Wang,&nbsp;Yi Deng,&nbsp;Haijuan Zhang,&nbsp;Kailong Guan,&nbsp;Xin Wang,&nbsp;Hongyu Li,&nbsp;Yang Li","doi":"10.1007/s00203-025-04471-4","DOIUrl":"10.1007/s00203-025-04471-4","url":null,"abstract":"<div><p><i>Pseudomonas aeruginosa</i> is a Gram-negative bacterium and a major opportunistic pathogen that can cause extensive acute and chronic infections. β-lactam antibiotics are the most commonly used prescription antibiotics worldwide and play a crucial role in the treatment of <i>Pseudomonas aeruginosa</i> infections. However, antibiotic resistance (AMR) is a global challenge. The β-lactam resistance in Gram-negative bacteria is due to the production of β-lactamases, including extended-spectrum β-lactamases, metallo-β-lactamases, and carbapenem-hydrolyzing class D β-lactamases. To restore the efficacy of this type of antibiotic, the most effective strategy is to combine it with β-lactamase inhibitors (BLI). In this study, we were pleasantly surprised to find that the quorum sensing inhibitor 2-(4-bromophenyl)-N-(2-oxotetrapyridinefuran-3-yl) butanamide (compound No.10) of <i>Pseudomonas aeruginosa</i>, when combined with β -lactam antibiotics, not only could inhibit the formation of biofilms in the standard and clinical strains of <i>Pseudomonas aeruginosa</i>, but also promoted the entry of antibiotics into the bacteria to exert their bactericidal effects. Moreover, it can also inhibit the expression of the drug resistance gene <i>ampc</i> in <i>Pseudomonas aeruginosa</i>, thereby suppressing the degradation effect of β-lactamase on β-lactam antibiotics.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing the adhesion potential of Bifidobacterium animalis subsp. lactis MCM-B-2007 via in vitro and in silico analyses","authors":"Vaidehi Pisu,&nbsp;Neelam Kapse,&nbsp;Deepa Shetty,&nbsp;Shilpa Wagh,&nbsp;Sumit Singh Dagar,&nbsp;Prashant K. Dhakephalkar","doi":"10.1007/s00203-025-04469-y","DOIUrl":"10.1007/s00203-025-04469-y","url":null,"abstract":"<div><p><i>Bifidobacterium</i> strains are widely recognized for their probiotic properties, and their ability to adhere to the intestinal epithelium is crucial for effective gut colonization and interaction with the host. This study offers a thorough examination of the adhesion capabilities of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i>, integrating in silico analyses with in vitro adhesion assessments. Genome analysis of MCM-B-2007 identified several adhesion proteins that might act as potential mediators of crosstalk between microbes and their host. Molecular docking studies further assessed these adhesion proteins interaction with gut mucins. MCM-B-2007 displayed the highest affinity for binding to MUC4, with a predicted weighted score of -1539.5. These adhesion capabilities were subsequently validated through in vitro adhesion assays. MCM-B-2007 demonstrated a significant adhesion potential, with an adhesion efficiency of approximately 306 ± 20 bacterial cells per 100 HT-29 cells, classifying it as a strongly adhesive strain. Additionally, MCM-B-2007 demonstrated the strong competitive inhibition of adhesion of pathogenic <i>Klebsiella pneumoniae</i> and <i>Salmonella</i> Typhimurium, effectively safeguarding the host cells against infections. These findings support its use as a probiotic with the potential for future application in establishing healthy gut microbiota.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145073764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The GrrA/GrrS system regulates Serratia plymuthica IC1270 surface proteins that induce expression of the host cell α5β1 integrin involved in the invasion of these bacteria","authors":"Olga Tsaplina,&nbsp;Tatiana Artamonova,&nbsp;Mikhail Khodorkovsky,&nbsp;Inessa Khmel","doi":"10.1007/s00203-025-04475-0","DOIUrl":"10.1007/s00203-025-04475-0","url":null,"abstract":"<div><p>The rhizobacteria <i>Serratia plymuthica</i> are considered promising antimicrobial agents. However, cases of infection of both plants and humans by these bacteria have been described. espite the various mechanisms of infection of plants and humans, cases cross-kingdom pathogenicity of bacteria are increasingly being recorded. The aim of the work was to assess the possibility of infection of human cells by <i>S. plymuthica</i>. Using confocal microscopy and a quantitative microbiological method, we showed for the first time that <i>S. plymuthica</i> IC1270 is able to penetrate M-HeLa cells. And the Quorum Sensing system and the global gene regulation system GrrA/GrrS determine the intensity of their invasion. Surprisingly, inactivation of the <i>grrA</i> gene reduces invasion, in contrast to inactivation of the <i>grrS</i> gene, which increases invasion. For penetration, these bacteria use the host cell α5β1 integrin, the intensity of expression of which increases as a result of infection. Moreover, the intensity of invasion upon gene inactivation correlate with the intensity of expression of α5 and β1 integrin subunits. The increased expression of these genes is due to contact with the <i>Serratia</i> surface protein OmpX, which is regulated by GrrA and GrrS. An additional factor that enhances invasion upon inactivation of the <i>grrS</i> gene may be the accumulation of isomerase PpiA, which mediates the folding of surface proteins. Thus, <i>S. plymuthica</i> penetrates M-HeLa cells using α5β1 integrin, the expression of which is regulated by the GrrA/GrrS-dependent surface protein of bacteria.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145073765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel mechanistic study on inhibiting influenza A virus replication by a newly extracted polypeptide targeting host autophagy","authors":"Nedaa Alalem,&nbsp;Mai Alalem,&nbsp;Ahmed Awad,&nbsp;Amany M. Elshamy,&nbsp;Osama R. Elalem,&nbsp;Ahmed M. Tabl,&nbsp;Mohamed E. Ebaid,&nbsp;Hany Khalil","doi":"10.1007/s00203-025-04476-z","DOIUrl":"10.1007/s00203-025-04476-z","url":null,"abstract":"<div><p>Influenza is a contagious respiratory disease in humans, primarily caused by influenza A and B viruses. The severity of pandemic influenza is influenced by several factors, including the virus’s ability to evade immune defenses such as autophagy, a key catabolic process within cells. Influenza A virus (IAV) exploits the autophagic pathway to facilitate its replication. In this study, we explored the impact of specific purified honey-derived peptide (H-P) on IAV-induced autophagy signaling, aiming to disrupt viral replication through host-directed strategies and potentially reduce the risk of viral mutation and resistance, as seen with antiviral drugs like Tamiflu. We examined the antiviral potential of H-P in human lung epithelial cells (A549) and compared its effects with Tamiflu and phosphate-buffered saline (PBS) as controls. To assess the safety of H-P, we first evaluated cell viability, lactate dehydrogenase (LDH) release, cell morphology, and cell count following treatment with various concentrations of H-P or Tamiflu in the absence of infection. At lower concentrations, including 5 µM, both treatments showed minimal impact on cell viability, LDH levels, morphology, and cell count. Upon infection with IAV, treatment with 5 µM H-P significantly reduced the expression of both viral nonstructural protein 1 (NS1) and nucleoprotein (NP), whereas Tamiflu treatment at the same concentration reduced only NP expression. Mechanistic analyses revealed that H-P induced both early and late apoptotic signaling in infected cells, as demonstrated by Annexin V staining, suggesting its role in promoting apoptosis during early stages of infection. Additionally, H-P treatment significantly suppressed the expression of autophagy-related genes <i>Atg5</i> and <i>LC3B</i> during the early stages of infection. It also inhibited the conversion of cytosolic LC3-I to membrane-bound LC3-II, even when autophagy was induced by rapamycin independently of infection. Notably, unlike Tamiflu, H-P increased the production of immune mediators, interferon-beta (IFN-β) and interleukin-6 (IL-6) specifically in response to IAV infection, without altering their expression in the absence of infection. Collectively, these findings highlight the antiviral potential of the naturally derived H-P polypeptide through suppression of IAV-stimulated autophagy, promotion of early apoptosis, and enhancement of antiviral cytokine responses. This suggests a promising host-targeted approach to influenza treatment that may complement or offer alternatives to conventional antiviral therapies like Tamiflu.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145073766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyanobacterial scytonemin, a potential photoprotective natural pigment: biomedical, industrial and environmental applications","authors":"Akshaya Kumar Behera,&nbsp;Sudhamayee Parida,&nbsp;Amiya Kumar Mandal,&nbsp;Srimanta Patra,&nbsp;Mrutyunjay Jena","doi":"10.1007/s00203-025-04462-5","DOIUrl":"10.1007/s00203-025-04462-5","url":null,"abstract":"<div><p>A pigment called scytonemin is produced by cyanobacteria under stress conditions. It is a lipid-soluble, yellow-brown indole-alkaloid pigment that is mostly found in the extracellular polysaccharide (EPS) sheaths or capsules of certain cyanobacteria. During radiation exposure, it has the potential to protect cyanobacteria from damaging UV rays due to its photoprotective properties. Because of its stability and durability, scytonemin is a good option for sunscreen compositions. Additionally, the antioxidant properties possessed by this compound help in scavenging reactive oxygen species, which is beneficial in creating medications to treat diseases linked to oxidative stress. Its stability makes it a promising biomarker for palaeoclimatological reconstructions and highlights its importance as a biosignature in astrobiology. Furthermore, its recently revealed anti-inflammatory and powerful antiproliferative capabilities, particularly the suppression of cell cycle-regulating kinases, make it an attractive pharmacophore for creating innovative therapies for hyperproliferative illnesses such as cancer. Its durability and distinct spectroscopic signal highlight its importance as a biosignature in astrobiology. Continued study into its biosynthesis, various functions, and scalable manufacturing methods is critical for its future commercialization and wider application in human welfare. This review discusses a brief overview of the biochemistry and biosynthesis of scytonemin, focusing on the biomedical, industrial, and environmental applications.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isothermal microcalorimetry analysis of microencapsulated Yamadazyma mexicana LPa14: release and antifungal activity against Colletotrichum gloeosporioides","authors":"Katia Nayely González-Gutiérrez,&nbsp;Leonardo Covarrubias-Rivera,&nbsp;Juan Arturo Ragazzo-Sánchez,&nbsp;Rafael López-Cruz,&nbsp;Montserrat Calderón-Santoyo,&nbsp;Katia Nayely","doi":"10.1007/s00203-025-04470-5","DOIUrl":"10.1007/s00203-025-04470-5","url":null,"abstract":"<div><p>Anthracnose caused by <i>Colletotrichum</i> is the most significant disease in avocado. Since its quiescent infection begins in the field, preharvest control is crucial. Applying microencapsulated <i>Yamadazyma mexicana</i> LPa14 in preharvest may prevent pathogen establishment and reduce production losses. However, the evaluation of the release and the antagonistic activity of microencapsulated yeasts is essential to ensure the efficacy of a bioformulation. Due to the limitations of traditional techniques, isothermal microcalorimetry (IMC) is proposed as an innovative, accurate, and sensitive technique for measuring the heat generated during biological processes. This work aimed to evaluate the release of microencapsulated <i>Y. mexicana</i> and its antifungal activity against <i>Colletotrichum gloeosporioides</i> from avocado fruit using IMC and traditional microbiological techniques. The intermediate release rate of the bioformulation was compared with low and high release rate treatments, and Pearson correlation was used to associate the release rates with the thermokinetic parameters. For the confrontation test, the thermogenic curves of yeast (1.14 × 10⁶ and 3.16 × 10⁷ cells/mL) and fungus were recorded, the co-culture curves were deconvoluted and the thermokinetic parameters: lag phase (λ), maximum growth rate (µ_Max), total heat (<i>Q</i>_t), and time to peak (T_p) were obtained. The release rates were inversely correlated with <i>Q</i>_t. In vitro, <i>C. gloeosporioides</i> inhibition was yeast concentration-dependent, with no differences between fresh (56.78–68.24%) and microencapsulated (54.21–70.90%) yeast, as well as for the thermokinetic values. In the confrontation assay, at 3.16 × 10⁷ cells/mL, the yeast and confrontation curves showed a similar pattern, with no significant differences between µ_Max, λ phase, <i>Q</i>_t, and T_p, indicating complete fungal inhibition. Instead, at 1.14 × 10⁶ cells/mL, the yeast and fungus growth is affected, indicating incomplete inhibition below this concentration. In conclusion, the IMC is suitable for in vitro testing, as it provides insights into the release dynamics and antifungal activity of microencapsulated biocontrol agents. This study is crucial for enhancing the efficacy and practical application of bioformulations for fungal diseases management.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial type IV secretion systems and spread of antimicrobial resistance: a study of potential inhibitors to T4SS-based resistance spread","authors":"Monojit Kumar Roy,&nbsp;Abhilash Bhattacharjee,&nbsp;Anil Kumar Singh","doi":"10.1007/s00203-025-04465-2","DOIUrl":"10.1007/s00203-025-04465-2","url":null,"abstract":"<div><p>Antimicrobial resistance (AMR) is a major global health threat, mainly driven by the rapid spread of resistance genes through horizontal gene transfer (HGT). The Type IV Secretion System (T4SS) acts as a crucial molecular machinery that facilitates this process, allowing bacteria to transfer DNA, effector proteins, and virulence factors. This review systematically explores the structural and functional diversity of T4SS, its role in spreading AMR, and current methods for its inhibition. T4SS consists of a multi-protein complex that spans bacterial membranes, mediating conjugative plasmid transfer, host-pathogen interactions, and bacterial competition. Key components include ATPases, pilus structures, and membrane-associated proteins that show both conserved features and species-specific adaptations. These traits enable functional specialization across Gram-positive and Gram-negative bacteria, significantly contributing to the spread of vital resistance genes like extended-spectrum β-lactamases and carbapenemases via mobile genetic elements. Several approaches have been developed to inhibit T4SS and combat AMR. Small molecules targeting ATPase activity or protein interactions are promising, as are natural phytochemicals that interfere with conjugation. Bacteriophage therapy provides another strategy by specifically targeting plasmid-carrying bacteria. Host immune responses, such as innate immune recognition and secretory immunoglobulins, also show potential to influence T4SS activity. Although progress has been made, challenges remain, especially in developing selective inhibition methods that do not harm beneficial microbiota or host cells. Future research should focus on high-resolution structural studies to support rational drug design and preclinical testing of combination therapies that include T4SS inhibitors with existing antibiotics. Gaining a deeper understanding of T4SS regulation and host-pathogen interactions will be vital for creating targeted AMR strategies that also maintain ecological balance.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145062324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative immunogenicity study of two different types of tuberculosis vaccines based on a heterologous boosting strategy","authors":"Xiaochun Wang,&nbsp;Runlin Wang,&nbsp;Jianghong Wang,&nbsp;Zian Zhang,&nbsp;LingYun Kong,&nbsp;Lu Xia,&nbsp;Zhiyang Qi,&nbsp;Haoying Chen","doi":"10.1007/s00203-025-04463-4","DOIUrl":"10.1007/s00203-025-04463-4","url":null,"abstract":"<div><p>BCG, one of the oldest vaccines in clinical use, has demonstrated well-documented safety, quality, and efficacy in preventing severe forms of tuberculosis (TB) in neonates. However, its protective efficacy declines significantly in adulthood, failing to prevent pulmonary the TB —a major driver of global TB transmission. To address this limitation, this study systematically evaluated two novel BCG-boosting strategies: a recombinant subunit protein vaccine targeting the Rv2074 antigen and a DNA vaccine encoding the same antigen, both evaluated in murine immunization. Antigen-specific cytokine levels in splenocyte supernatants and serum antibody titers were quantified by ELISA after euthanizing mice at 8 weeks (8w) and 16 weeks (16w) post-immunization. The results indicated that both vaccine types induced robust Th1-type immune responses in mice. Additionally, antigen-specific T cell cytokine secretion was analyzed using flow cytometry combined with intracellular cytokine staining. Experimental data revealed that the BCG + P group exhibited a significant increase in CD4⁺ T cells, while the BCG + D group showed a higher proportion of CD8⁺ T cells.Long-term immune effects surpassing short-term outcomes in both groups. These findings suggest that both vaccine types show promise as BCG-based booster vaccines.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145057661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the novel Cutibacterium acnes phage KIT08 and its associated pseudolysogenic bacterial isolate","authors":"Phuoc-Dung Nguyen,&nbsp;Koki Nakanishi,&nbsp;Chihiro Hosokawa,&nbsp;Nguyen Song Han,&nbsp;Masao Kitao,&nbsp;Masanao Yoshimoto,&nbsp;Kaeko Kamei","doi":"10.1007/s00203-025-04451-8","DOIUrl":"10.1007/s00203-025-04451-8","url":null,"abstract":"<div><p><i>Cutibacterium acnes</i>, formerly <i>Propionibacterium</i> acnes, is a Gram-positive bacterium commonly recognized as an important factor in acne vulgaris and infections associated with prosthetic medical devices. With the rise in antibiotic resistance, phage therapy has gained renewed attention as a promising alternative to antibiotics. In addition to a strict lytic cycle, some virulent phages may enter a pseudolysogenic state and exclude superinfections, thereby significantly limiting the applicability of these potential antimicrobial agents. However, the trade-off induced by phage infection of bacterial cells during this state and its molecular mechanism are yet to be confirmed, especially for <i>C. acnes</i> phages. In this study, a novel <i>Cutibacterium acnes</i> phage, KIT08, was isolated and characterized. It demonstrated rapid infectivity and moderately strong bacteriolysis. After infection of <i>C. acnes</i> NBRC 107,605, pseudolysogenic bacteria were collected and examined for physiological tradeoffs. The pseudolysogenic isolate exhibited slower growth and downregulation of the transcriptional levels of biofilm-producing genes, such as <i>lipase 2</i> and <i>hyaluronate lyase</i>, leading to a decrease in biofilm formation. Additionally, a genomic study of phage KIT08 revealed that open reading frames 23 and 34 encode putative proteins homologous to repressor C and LTP proteins, which may play an important role in the induction of pseudolysogeny and superinfection exclusion in <i>C. acnes</i>.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-025-04451-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New observations in the spores and hyphae of Colletotrichum siamense exposed to nanochitosan particles","authors":"Nixe Adriana Hernández-López,&nbsp;Maribel Plascencia-Jatomea,&nbsp;Jaime Lizardi-Mendoza,&nbsp;Miguel Ángel Martínez-Téllez,&nbsp;Carmen María López-Saiz,&nbsp;Eber Addí Quintana-Obregón","doi":"10.1007/s00203-025-04466-1","DOIUrl":"10.1007/s00203-025-04466-1","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Colletotrichum siamense</i> is a complex phytopathogen within the <i>Colletotrichum gloeosporioides</i> species, responsible for anthracnose disease in horticultural crops, leading to significant economic losses. Synthetic fungicides are frequently used for control; however, their overuse has resulted in fungal resistance and environmental contamination due to residues accumulation, underscoring the necessity for safer and more effective alternatives. Chitosan nanoparticles provide a feasible alternative with notable antifungal activity against various phytopathogenic fungi. Nonetheless, despite their efficacy against numerous fungi, certain species within the <i>C. gloeosporioides</i> complex have demonstrated reduced susceptibility to the biopolymer, suggesting potential adaptation mechanisms to chitosan nanoparticles. This study assessed the in vitro antifungal efficacy of chitosan nanoparticles against <i>C. siamense</i>. The findings indicated that the biopolymer suppressed mycelial growth by 21.73%. The germination of the spores was delayed by 2 h, accompanied by a decrease in germinative tube length relative to the control group. Nevertheless, an acceleration in growth was observed, resulting in 100% of the spores germinating concurrently with the control group. Furthermore, it was noted that the integrity of both the plasmatic membrane and the cell wall was compromised by the nanochitosan. These findings facilitate the investigation of potential adaptive or defensive mechanisms that <i>C. siamense</i> may employ in response to nanochitosan, offering novel insights for disease management.</p>\u0000 </div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 10","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145021500","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}