Archives of Microbiology最新文献_第5页

Improving the activity of an inulosucrase by rational engineering for the efficient biosynthesis of low-molecular-weight inulin 通过合理工程改善菊粉酶的活性，以高效生物合成低分子量菊粉。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-03 DOI: 10.1007/s00203-024-04153-7

Dawei Ni, Zhaolin Huang, Shuqi Zhang, Yang Yang, Xiaoyong Liu, Wei Xu, Wenli Zhang, Wanmeng Mu

{"title":"Improving the activity of an inulosucrase by rational engineering for the efficient biosynthesis of low-molecular-weight inulin","authors":"Dawei Ni, Zhaolin Huang, Shuqi Zhang, Yang Yang, Xiaoyong Liu, Wei Xu, Wenli Zhang, Wanmeng Mu","doi":"10.1007/s00203-024-04153-7","DOIUrl":"10.1007/s00203-024-04153-7","url":null,"abstract":"<div>Inulin, a widely recognized prebiotic, has diverse applications across various industrial sectors. Although inulin is primarily produced through plant extraction, there is growing interest in enzymatic synthesis as an alternative. The enzymatic production of inulin from sucrose, which yields polymers with degrees of polymerization similar to those of plant-derived inulin, shows potential as a viable replacement for traditional extraction methods. In this study, an inulosucrase from Neobacillus bataviensis was identified, demonstrating a non-processive mechanism specifically tailored for synthesizing inulin with polymerization degrees ranging from 3 to approximately 40. The enzyme exhibited optimal activity at pH 6.5 and 55 °C, efficiently producing inulin with a yield of 50.6%. Ca2+ can improve the activity and thermostability of this enzyme. To enhance catalytic total activity, site-directed and truncated mutagenesis techniques were applied, resulting in the identification of a mutant, T149S, displaying a significant 57% increase in catalytic total activity. Molecular dynamics simulations unveiled that the heightened flexibility observed in three surface regions positively influenced enzymatic activity. This study not only contributes to the theoretical foundation for inulosucrase engineering but also presents a potential avenue for the production of inulin.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, characterization of β-glucosidase from Furfurilactobacillus rossiae in bioconversion and its efficacy 罗氏糠乳杆菌β-葡萄糖苷酶的克隆、生物转化特性及其功效。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-03 DOI: 10.1007/s00203-024-04148-4

Thi Ngoc Anh Tran, Jinnatun Nahar, Jin-Kyu Park, Mohanapriya Murugesan, Jae-Heung Ko, Jong Chan Ahn, Deok-Chun Yang, Ramya Mathiyalagan, Dong Uk Yang

{"title":"Cloning, characterization of β-glucosidase from Furfurilactobacillus rossiae in bioconversion and its efficacy","authors":"Thi Ngoc Anh Tran, Jinnatun Nahar, Jin-Kyu Park, Mohanapriya Murugesan, Jae-Heung Ko, Jong Chan Ahn, Deok-Chun Yang, Ramya Mathiyalagan, Dong Uk Yang","doi":"10.1007/s00203-024-04148-4","DOIUrl":"10.1007/s00203-024-04148-4","url":null,"abstract":"<div>Minor ginsenosides produced by β-glucosidase are interesting biologically and pharmacologically. In this study, new ginsenoside-hydrolyzing glycosidase from Furfurilactobacillus rossiae DCYL3 was cloned and expressed in Escherichia coli strain BL21. The enzyme converted Rb1 and Gyp XVII into Rd and compound K following the pathways: Rb1→Rd and Gyp XVII→F2→CK, respectively at optimal condition: 40 °C, 15 min, and pH 6.0. Furthermore, we examined the cytotoxicity, NO production, ROS generation, and gene expression of Gynostemma extract (GE) and bioconverted Gynostemma extract (BGE) in vitro against A549 cell lines for human lung cancer and macrophage RAW 264.7 cells for antiinflammation, respectively. As a result, BGE demonstrated significantly greater toxicity than GE against lung cancer at a dose of 500 µg/mL but in normal cells showed lower toxicity. Then, we indicated an enhanced generation of ROS, which may be boosting cancer cell toxicity. By blocking the intrinsic way, BGE increased p53, Bax, Caspase 3, 9, and while Bcl2 is decreased. At 500 µg/mL, the BGE sample was less toxic in normal cells and decreased the LPS-treated NO and ROS level to reduce inflammation. In addition, BGE inhibited the expression of pro-inflammatory genes COX-2, iNOS, IL-6, and IL-8 in RAW 264.7 cells than the sample of GE. In conclusion, FrBGL3 has considerable downstream applications for high-yield, low-cost, effective manufacture of minor ginsenosides. Moreover, the study’s findings imply that BGE would be potential materials for anti-cancer and anti-inflammatory agent after consideration of future studies.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142364205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

XylR regulates genes at xyl cluster, involved in D-xylose catabolism in Herbaspirillum seropedicae Z69 XylR 调节 xyl 簇上的基因，参与 Herbaspirillum seropedicae Z69 中 D-木糖的分解代谢。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-01 DOI: 10.1007/s00203-024-04143-9

Ana Karen Malán, Juan José Marizcurrenaa, Manuela Oribe, Susana Castro-Sowinski, Silvia Batista

{"title":"XylR regulates genes at xyl cluster, involved in D-xylose catabolism in Herbaspirillum seropedicae Z69","authors":"Ana Karen Malán, Juan José Marizcurrenaa, Manuela Oribe, Susana Castro-Sowinski, Silvia Batista","doi":"10.1007/s00203-024-04143-9","DOIUrl":"10.1007/s00203-024-04143-9","url":null,"abstract":"<div>D-xylose, one of the most abundant sugars in lignocellulosic biomass, is not widely used to produce bioproducts with added value, in part due to the absence of industrial microorganisms able to metabolize it efficiently. Herbaspirillum seropedicae Z69 is a β-proteobacterium able to accumulate poly-3-hydroxybutyrate, a biodegradable thermoplastic biopolymer, with contents higher than 50%. It metabolizes D-xylose by non-phosphorylative pathways. In the genome of Z69, we found the genes xylFGH (ABC D-xylose transporter), xylB, xylD, and xylC (superior non-phosphorylative pathway), and the transcriptional regulator xylR, forming the xyl cluster. We constructed the knock-out mutant Z69ΔxylR that has a reduced growth in D-xylose and in D-glucose, compared with Z69. In addition, we analyzed the expression of xyl genes by RT-qPCR and promoter fusion. These results suggest that XylR activates the expression of genes at the xyl cluster in the presence of D-xylose. On the other hand, XylR does not regulate the expression of xylA, mhpD (lower non-phosphorylative pathways) and araB (L-arabinose dehydrogenase) genes. The participation of D-glucose in the regulation mechanism of these genes must still be elucidated. These results contribute to the development of new strains adapted to consume lignocellulosic sugars for the production of value-added bioproducts.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam 揭示与越南中部高原地区自然发生的木犀属枯萎病和茎腐病有关的霉菌。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-27 DOI: 10.1007/s00203-024-04145-7

Dung Le

{"title":"Unveiling Fusaria mycoflora associated with natural occurrence of lisianthus wilt and stem rot in Central-highland Vietnam","authors":"Dung Le","doi":"10.1007/s00203-024-04145-7","DOIUrl":"10.1007/s00203-024-04145-7","url":null,"abstract":"<div>Wilt and stem rot (WSR) is an emerging syndrome threatening cut lisianthus (Eustoma russellianum) production in Lam Dong province, Vietnam. The disease was observed in all 13 inspected commercial lisianthus greenhouses across major lisianthus cultivation areas in Lam Dong, including Da Lat, Lac Duong, Don Duong, and Duc Trong, with incidence increasing with plant age, ranging from 7.5 to 32.4%. Infected plants displayed stunting, wilting, stem rot and blight, and dieback, with predominance of wilt and stem rot. The disease showed polycyclic behavior, with symptoms shifting from random or scattered in young plants to clustered patterns after the initial flower cutting. Forty-one Fusaria-like fungal isolates recovered from diseased lisianthus plants were identified as Fusarium vanleeuwenii (28 isolates), Neocosmospora solani (11 isolates), and F. annulatum (2 isolates) based on morphological observations and phylogenetic analysis of the internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF-1α) genes. The composition of Fusaria species varied across sites, with F. vanleeuwenii being consistently present. Pathogenicity tests confirmed that isolates of F. vanleeuwenii Li-Fo9511, N. solani Li-Fs4311, and F. annulatum Li-Fp3051 caused typical stem rot in in-vitro assays. In-planta assays showed wilting in seedlings starting two weeks post-infection, with a remarkable increase in disease incidence and severity between five and six weeks, particularly for F. vanleeuwenii Li-Fo9511. The pathogens were re-isolated and morphologically confirmed, fulfilling Koch’s postulates. This is the first report of F. vanleeuwenii, N. solani, and F. annulatum as pathogens of lisianthus WSR in Vietnam, highlighting the need for effective control strategies.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340031","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combined effect of polyphosphate kinase and lon protease in Streptomyces coelicolor A3(2) antibiotic production 多磷酸激酶和长蛋白酶在共生链霉菌 A3（2）抗生素生产中的联合作用。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-27 DOI: 10.1007/s00203-024-04138-6

Nagihan Genel, Sedef Tunca

引用次数: 0

Retraction Note: Gorillibacterium timonense sp. nov., isolated from an obese patient 撤稿说明：从一名肥胖患者体内分离出的Gorillibacterium timonense sp.

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04146-6

Sokhna Ndongo, Mamadou Beye, Noémie Labas, Fabrizio Di Pinto, Magali Richez, Jean-Christophe Lagier, Pierre-Edouard Fournier, Didier Raoult, Fadi Bittar

引用次数: 0

Role of grass endophytic fungi as a natural resource of bioactive metabolites 草内生真菌作为生物活性代谢物自然资源的作用。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04132-y

R. Nischitha

{"title":"Role of grass endophytic fungi as a natural resource of bioactive metabolites","authors":"R. Nischitha","doi":"10.1007/s00203-024-04132-y","DOIUrl":"10.1007/s00203-024-04132-y","url":null,"abstract":"<div>Grass endophytic fungi have garnered increasing attention as a prolific source of bioactive metabolites with potential application across various fields, including pharmaceticals agriculture and industry. This review paper aims to synthesize knowledge on the diversity, isolation, and bioactivity of metabolites produced by grass endophytic fungi. Additionally, this approach aids in the conservation of rare and endangered plant species. Advanced analytical techniques such as high-performance liquid chromatography, liquid chromatograpy-mass spectrometry and gas chromatography are discussed as critical tools for metabolite identification and characterization. The review also highlights significant bioactive metabolites discovered to date, emphasizing their antimicrobial, antioxidant, and insecticidal activities and plant growth regulation properties. Besides address the challenges and future prospects in harnessing grass endophytic fungi for sustainable biotenological applications. By consolidating recent advancements and identifying agaps in the current research, this paper provides a comprehensive overview of the potential grass endophytic fungi as a valuable resource for novel bioactive compounds.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Retraction Note: Gemella massiliensis sp. nov., a new bacterium isolated from the human sputum 撤稿说明：Gemella massiliensis sp.

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04147-5

Maxime Descartes Mbogning Fonkou, Cheikh Ibrahima Lo, Zouina Mekhalif, Melhem Bilen, Enora Tomei, Edmond Kuete Yimagou, Grégory Dubourg, Didier Raoult, Florence Fenollar, Pierre-Edouard Fournier

引用次数: 0

Genomic identification of Oryctes rhinoceros nudivirus isolates, a biocontrol agent for coconut rhinoceros beetle 椰子犀角金龟子生物控制剂 Oryctes rhinoceros nudivirus 分离物的基因组鉴定。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-26 DOI: 10.1007/s00203-024-04116-y

Ela Hiszczynska-Sawicka, Mitchell K. Weston, Aurelie Laugraud, Charles A. Hefer, Jeanne M. E. Jacobs, Sean D. G. Marshall

{"title":"Genomic identification of Oryctes rhinoceros nudivirus isolates, a biocontrol agent for coconut rhinoceros beetle","authors":"Ela Hiszczynska-Sawicka, Mitchell K. Weston, Aurelie Laugraud, Charles A. Hefer, Jeanne M. E. Jacobs, Sean D. G. Marshall","doi":"10.1007/s00203-024-04116-y","DOIUrl":"10.1007/s00203-024-04116-y","url":null,"abstract":"<div>The coconut rhinoceros beetle (Oryctes rhinoceros, CRB) is a serious pest of coconut and oil palms. It is native to South and Southeast Asia and was inadvertently introduced to Samoa in 1909. It has invaded many other Pacific countries throughout the last century. Oryctes rhinoceros nudivirus (OrNV), a natural pathogen of CRB in its native range, was successfully introduced as a classical biocontrol agent and has effectively suppressed invasive CRB populations for decades. However, resurgence of CRB has been recorded, with new invasions detected in several Pacific Island Countries and Territories. Additionally, new populations of CRB are emerging in some invaded areas that have a degree of resistance to the virus isolates commonly released for CRB biocontrol. Here, we designed a fast and reliable tool for distinguishing between different OrNV isolates that can help with the selection process to identify effective isolates for management of new CRB invasions. A comparison of 13 gene/gene region sequences within the OrNV genome of 16 OrNV isolates from native and invaded ranges allowed us to identify unique Single Nucleotide Polymorphisms (SNPs). With these SNPs, we developed an assay using multiplex PCR-amplicon-based nanopore sequencing to distinguish between OrNV isolates. We found that as few as four gene fragments were sufficient to identify 15 out of 20 OrNV isolates. This method can be used as a tool to monitor the establishment and distribution of OrNV isolates selected for release as biocontrol agents in CRB-infected areas.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11427517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening of promising molecules against potential drug targets in Yersinia pestis by integrative pan and subtractive genomics, docking and simulation approach 通过整合泛基因组学和减基因组学、对接和模拟方法，筛选针对鼠疫耶尔森菌潜在药物靶点的有前景分子。

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-09-25 DOI: 10.1007/s00203-024-04140-y

Lei Chen, Lihu Zhang, Yanping Li, Liang Qiao, Suresh Kumar

{"title":"Screening of promising molecules against potential drug targets in Yersinia pestis by integrative pan and subtractive genomics, docking and simulation approach","authors":"Lei Chen, Lihu Zhang, Yanping Li, Liang Qiao, Suresh Kumar","doi":"10.1007/s00203-024-04140-y","DOIUrl":"10.1007/s00203-024-04140-y","url":null,"abstract":"<div>This study focuses on Yersinia pestis, the bacterium responsible for plague, which posed a severe threat to public health in history. Despite the availability of antibiotics treatment, the emergence of antibiotic resistance in this pathogen has increased challenges of controlling the infections and plague outbreaks. The development of new drug targets and therapies is urgently needed. This research aims to identify novel protein targets from 28 Y. pestis strains by the integrative pan-genomic and subtractive genomics approach. Additionally, it seeks to screen out potential safe and effective alternative therapies against these targets via high-throughput virtual screening. Targets should lack homology to human, gut microbiota, and known human ‘anti-targets’, while should exhibit essentiality for pathogen’s survival and virulence, druggability, antibiotic resistance, and broad spectrum across multiple pathogenic bacteria. We identified two promising targets: the aminotransferase class I/class II domain-containing protein and 3-oxoacyl-[acyl-carrier-protein] synthase 2. These proteins were modeled using AlphaFold2, validated through several structural analyses, and were subjected to molecular docking and ADMET analysis. Molecular dynamics simulations determined the stability of the ligand-target complexes, providing potential therapeutic options against Y. pestis.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142340030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0