One-step narrow-thermal-cycling strand exchange amplification for sensitive detection of porcine reproductive and respiratory syndrome virus

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-05-22 DOI:10.1007/s00203-025-04351-x

Linlin Zhuang, Chunlei Song, Li Sun, Haiqiang Xie, Liqun Wang, Qingxin Liu, Hongjing Shi, Jianbo Yang, Qiuping Shen

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引用次数: 0

Abstract

Porcine reproductive and respiratory syndrome (PRRS), which is primarily characterized by respiratory and reproductive dysfunction, is an epidemic disease caused by porcine reproductive and respiratory syndrome virus (PRRSV) that has the potential to economically devastate the global swine industry. Rapid and accurate detection of PRRSV is critical for effective control of PRRS in swine. In this study, a novel one-step, highly sensitive and specific accelerated strand exchange amplification (ASEA) method for the detection of PRRSV was developed. The detection limit of the ASEA method was determined to be 6 copies µL^–1 of PRRSV RNA reference material or PRRSV in spiked swine blood. The ASEA method demonstrated the capacity to discern the currently circulating PRRSV genotypes without cross-reactivity with other porcine-derived pathogens that manifest similar clinical signs. The ASEA method exhibited a detection time of 35 min, and its clinical applicability was validated through the analysis of 5 inactivated blood samples and 62 clinical samples. The method’s potential extends beyond the diagnosis of PRRSV, as it can also be applied to the rapid diagnosis of other RNA pathogens. This capacity is expected to make significant contributions to future epidemic prevention and surveillance efforts.

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一步窄热循环链交换扩增灵敏检测猪繁殖与呼吸综合征病毒

猪繁殖与呼吸综合征（PRRS）是由猪繁殖与呼吸综合征病毒（PRRSV）引起的一种流行性疾病，主要表现为呼吸和生殖功能障碍，具有破坏全球养猪业经济的潜力。快速准确的PRRSV检测是有效控制猪PRRSV的关键。本研究建立了一种新的一步、高灵敏度和特异性的加速链交换扩增（ASEA）检测PRRSV的方法。ASEA方法的检出限为6 copiesµL-1 PRRSV参比物或加标猪血中的PRRSV。ASEA方法能够识别当前流行的PRRSV基因型，而不会与其他表现出类似临床症状的猪源性病原体产生交叉反应。ASEA方法检测时间为35 min，通过对5份灭活血液样本和62份临床样本的分析，验证了其临床适用性。该方法的潜力超出了PRRSV的诊断，因为它也可以应用于其他RNA病原体的快速诊断。这种能力预计将对今后的流行病预防和监测工作作出重大贡献。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.