Archives of Microbiology最新文献_第6页

Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production 从枇杷皮中分离和鉴定附生的 Pichia kudriavzevii，并研究其用于白酒生产的发酵特性

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-19 DOI: 10.1007/s00203-024-04162-6

Qingfang Xu, Wei Huang, Yanqin Li, Jian Cai, Xiu Gao, Xu Bai, Weiliang Liu, Lifang Zhang, Ling Zhu

{"title":"Isolation and identification of epiphytic Pichia kudriavzevii from loquat peels and investigation of its fermentation characteristics for liquor production","authors":"Qingfang Xu, Wei Huang, Yanqin Li, Jian Cai, Xiu Gao, Xu Bai, Weiliang Liu, Lifang Zhang, Ling Zhu","doi":"10.1007/s00203-024-04162-6","DOIUrl":"10.1007/s00203-024-04162-6","url":null,"abstract":"<div>During the process of fruit wine production, yeast plays a crucial role in influencing the taste, flavor, and overall quality of the wine. This study aims to enhance the flavor and quality of loquat wine by isolating strains of Pichia kudriavzevii (P. kudriavzevii) with desirable winemaking characteristics from loquat fruit fermentation broth. A total of 12 strains of P. kudriavzevii were isolated and subjected to morphological and molecular biological identification. Their fermentation performance, ethanol production, ester production, hydrogen sulfide production, killer activity, and tolerance were evaluated. The results revealed that strains Q-2, Q-9, Q-10, Q-12, Q-20, and Q-42 exhibited robust growth and strong tolerance under conditions of 40 °C temperature, 12% ethanol concentration, 350 g/L glucose concentration, and pH 2.8. Strain Q-42 demonstrated the strongest gas production capacity, killer activity, and good ester and ethanol production. As a highly active fermentation strain with excellent wine making characteristics, P. kudriavzevii Q-42 provides a valuable yeast resource for the industrial production of loquat wine and offers technical support for improving the overall quality of loquat wine.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04162-6.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Assessing the effects of NapA gene overexpression on denitrification and denitrogenation in magnetospirillum gryphiswaldense MSR-1 评估NapA基因过表达对磁螺旋体MSR-1中反硝化和反氮化的影响

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-19 DOI: 10.1007/s00203-024-04158-2

Jiawei Hu, Meijing Liu, Lu Li, Jinjing Hu, Cong Wang

{"title":"Assessing the effects of NapA gene overexpression on denitrification and denitrogenation in magnetospirillum gryphiswaldense MSR-1","authors":"Jiawei Hu, Meijing Liu, Lu Li, Jinjing Hu, Cong Wang","doi":"10.1007/s00203-024-04158-2","DOIUrl":"10.1007/s00203-024-04158-2","url":null,"abstract":"<div>Previous research on Magnetospirillum gryphiswaldense MSR-1 found that MSR-1 has a good denitrification nitrogen removal ability and specific application prospects in the sewage biological nitrogen removal field. Therefore, this study selected the essential denitrification gene NapA in MSR-1-wt for overexpression, and the overexpressed MSR-1-NapA was successfully constructed. Q-PCR amplification experiment and AGAR gel electrophoresis experiment proved that the relative transcription level of the NapA gene was increased by more than four times, and the denitrification ability of MSR-1-wt and MSR-1-NapA was further determined by enzyme activity experiment, denitrification experiment, and flow cytometry. The results showed that overexpression of the NapA gene increased nitrate reductase activity in MSR-1-NapA by more than four times. In the solution with a nitrate concentration of 118.33 ± 3.23 mgN/L, the denitrification efficiency of MSR-1-NapA was superior to that of MSR-1-wt, significantly enhancing both the denitrification and nitrogen removal capacities of MSR-1. This indicates its greater potential for biological nitrogen removal in wastewater treatment.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: A rho-type GTPase activating protein affects the growth and development of Cordyceps cicadae 更正为一种Rho型GTP酶活化蛋白影响冬虫夏草的生长发育

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-18 DOI: 10.1007/s00203-024-04160-8

Xueqian Li, Yu Zou, Neeraj Shrivastava, Jiandong Bao, Fu-Cheng Lin, Hongkai Wang

引用次数: 0

Investigating role of positively selected genes and mutation sites of ERG11 in drug resistance of Candida albicans 研究 ERG11 的正选基因和突变位点在白色念珠菌耐药性中的作用

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-18 DOI: 10.1007/s00203-024-04159-1

Prayagraj Fandilolu, Chandan Kumar, Dushyant Palia, Susan Idicula-Thomas

{"title":"Investigating role of positively selected genes and mutation sites of ERG11 in drug resistance of Candida albicans","authors":"Prayagraj Fandilolu, Chandan Kumar, Dushyant Palia, Susan Idicula-Thomas","doi":"10.1007/s00203-024-04159-1","DOIUrl":"10.1007/s00203-024-04159-1","url":null,"abstract":"<div>The steep increase in acquired drug resistance in Candida isolates has posed a great challenge in the clinical management of candidiasis globally. Information of genes and codon sites that are positively selected during evolution can provide insights into the mechanisms driving antifungal resistance in Candida. This study aimed to create a manually curated list of genes of Candida spp. reported to be associated with antifungal resistance in literature, and further investigate the structure-function implications of positively selected genes and mutation sites. Sequence analysis of antifungal drug resistance associated gene sequences from various species and strains of Candida revealed that ERG11 and MRR1 of C. albicans were positively selected during evolution. Four sites in ERG11 and two sites in MRR1 of C. albicans were positively selected and associated with drug resistance. These four sites (132, 405, 450, and 464) of ERG11 are predictive markers for azole resistance and have evolved over time. A well-characterized crystal structure of sterol-14-α-demethylase (CYP51) encoded by ERG11 is available in PDB. Therefore, the stability of CYP51 in complex with fluconazole was evaluated using MD simulations and molecular docking studies for two mutations (Y132F and Y132H) reported to be associated with azole resistance in literature. These mutations induced high flexibility in functional motifs of CYP51. It was also observed that residues such as I304, G308, and I379 of CYP51 play a critical role in fluconazole binding affinity. The insights gained from this study can further guide drug design strategies addressing antimicrobial resistance.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142451127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation, identification, and technological characteristics analysis of yeast strains from Pyracantha fortuneana fruits fermentation liquid 从 Pyracantha fortuneana 果实发酵液中分离、鉴定和分析酵母菌株的技术特性

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-18 DOI: 10.1007/s00203-024-04164-4

Ling Zhu, Jiang-Yan Yu, Qing-Fang Xu, Xu Bai, Xiu Gao, Li-Fang Zhang, Wei-Liang Liu, Hao-Han Wang, Jian Cai

{"title":"Isolation, identification, and technological characteristics analysis of yeast strains from Pyracantha fortuneana fruits fermentation liquid","authors":"Ling Zhu, Jiang-Yan Yu, Qing-Fang Xu, Xu Bai, Xiu Gao, Li-Fang Zhang, Wei-Liang Liu, Hao-Han Wang, Jian Cai","doi":"10.1007/s00203-024-04164-4","DOIUrl":"10.1007/s00203-024-04164-4","url":null,"abstract":"<div>Pyracantha fortuneana (P. fortuneana), as a medicinal and edible plant resource, is rich in nutrients. In order to screen the high quality yeast used in Firebone fruit wine, 12 strains of yeast were isolated and purified from P. fortuneana fermentation broth by traditional pure culture method. They were identified by molecular biology as Pichia kudriavzevii (P. kudriavzevii) and Saccharomyces cerevisiae (S. cerevisiae), respectively. Strain HJ–2 could grow normally at 30℃, alcohol content 15%, SO2 mass concentration 360 mg/L, pH 3.2, sucrose mass concentration 400 g/L and glucose mass concentration 250 g/L. Strain HJ–6 could grow normally at 30℃, alcohol content 3%, SO2 concentration 360 mg/L, pH 3.2, sucrose concentration 250 g/L, glucose concentration 300 g/L. Based on the technological characteristics of fruit wine, S. cerevisiae HJ–2 has the potential of brewing P. fortuneana fruit wine. P. kudriavzevii HJ–6 has a low tolerance to ethanol and is suitable for the production of fermented beverages such as low–alcohol wine or beer.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142447436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streamlined boiling lysis DNA extraction for Gram-positive aquaculture pathogen Streptococcus agalactiae 简化革兰氏阳性水产养殖病原体无乳链球菌的沸腾裂解 DNA 提取过程

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-17 DOI: 10.1007/s00203-024-04163-5

Syahir Habib, Mohammad Noor Amal Azmai, Ina-Salwany Md Yasin, Noor Azlina Masdor, Nur Azura Mohd Said, Nur Adeela Yasid

{"title":"Streamlined boiling lysis DNA extraction for Gram-positive aquaculture pathogen Streptococcus agalactiae","authors":"Syahir Habib, Mohammad Noor Amal Azmai, Ina-Salwany Md Yasin, Noor Azlina Masdor, Nur Azura Mohd Said, Nur Adeela Yasid","doi":"10.1007/s00203-024-04163-5","DOIUrl":"10.1007/s00203-024-04163-5","url":null,"abstract":"<div>Accurate genetic analysis is essential for the detection of pathogens as it necessitates suitable DNA extraction methods tailored to specific microorganisms such as Gram-positive bacteria. This study examined several commercial and simplified DNA extraction methods for their suitability in isothermal downstream applications. Extracted DNA was assessed using spectrophotometry, electrophoresis, polymerase chain reaction (PCR) and loop-mediated isothermal amplification (LAMP) while its stability was inspected after five months of storage. The findings revealed variations in DNA yield, purity and integrity among the extraction methods. While extraction kits demonstrated high yield and purity, the in-house extraction techniques showed incoherent correlation between yield and purity, yet showed promise for a streamlined extraction process. The DNA acquired from all methods yielded positive amplification in PCR and LAMP. DNA extracted by kits exhibits prolonged stability than those obtained via boiling lysis. Both methods offer distinct advantages, with commercial kits providing longer stability and high-quality DNA while boiling lysis stands out for its simplicity, with shorter handling and processing periods. This study emphasizes selecting ideal extraction methods for Streptococcus agalactiae, in the prospect of aquaculture settings. In particular, successful LAMP reaction suggests that boiled extracts are feasible enough for detection, and suited for point-of-care (POC) testing where prompt detection of aquatic pathogens is often critical. Ultimately, the choice of method should be contemplated on a case-by-case basis such as the study goals, intended settings, and type of samples.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142443341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of microorganisms in Panax ginseng: cultivation of plants, and biotransformation and bioactivity of key component ginsenosides 微生物在人参中的应用：植物栽培以及主要成分人参皂苷的生物转化和生物活性

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-16 DOI: 10.1007/s00203-024-04144-8

Hongyu Ji, Lidong Guo, Dan Yu, Xiaowei Du

引用次数: 0

Characteristic of new Phaffia rhodozyma yeast strains isolated from birch slime fluxes in Poland 从波兰桦树粘液流中分离出的新 Phaffia rhodozyma 酵母菌菌株的特征

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-16 DOI: 10.1007/s00203-024-04161-7

Anna M. Kot, Katarzyna Pobiega, Marek Kieliszek, Katarzyna Michalak, Stanisław Błażejak

{"title":"Characteristic of new Phaffia rhodozyma yeast strains isolated from birch slime fluxes in Poland","authors":"Anna M. Kot, Katarzyna Pobiega, Marek Kieliszek, Katarzyna Michalak, Stanisław Błażejak","doi":"10.1007/s00203-024-04161-7","DOIUrl":"10.1007/s00203-024-04161-7","url":null,"abstract":"<div>Three new strains of Phaffia rhodozyma yeast have recently been isolated in Poland. The aim of this study was to phenotypically characterize these strains and to compare them with the properties of the reference strain. The potential for carotenoid biosynthesis in these strains was also determined, depending on temperature, carbon, and nitrogen sources in the medium. Phaffia rhodozyma yeasts were also identified by MALDI-TOF MS. There were minor differences in cell morphology among the strains. All strains reproduced asexually by budding and formed spherical chlamydospores. No ability for sexual reproduction was observed. Physiological tests showed minor variations between the reference strain and the isolates, likely due to the geographical specificity of the habitat from which they were originally isolated. Analysis of protein spectra showed that the tested yeast isolates had seven common peaks of different intensities, with masses at 2200, 2369, 3213, 3628, 3776, 3921, and 4710 m/z. Moreover, additional strain-dependent spectra were found. The amount of synthesized carotenoids varied with the carbon and nitrogen sources used, as well as the temperature. The best producer of carotenoids was the P. rhodozyma CMIFS 102 isolate.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04161-7.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofilm matrix: a multifaceted layer of biomolecules and a defensive barrier against antimicrobials 生物膜基质：多层生物分子和抗菌剂的防御屏障

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-14 DOI: 10.1007/s00203-024-04157-3

Harini Ragupathi, Mahamahima Muthuswamy Pushparaj, Sarves Mani Gopi, Deenadayalan Karaiyagowder Govindarajan, Kumaravel Kandaswamy

{"title":"Biofilm matrix: a multifaceted layer of biomolecules and a defensive barrier against antimicrobials","authors":"Harini Ragupathi, Mahamahima Muthuswamy Pushparaj, Sarves Mani Gopi, Deenadayalan Karaiyagowder Govindarajan, Kumaravel Kandaswamy","doi":"10.1007/s00203-024-04157-3","DOIUrl":"10.1007/s00203-024-04157-3","url":null,"abstract":"<div>Bacterial cells often exist in the form of sessile aggregates known as biofilms, which are polymicrobial in nature and can produce slimy Extracellular Polymeric Substances (EPS). EPS is often referred to as a biofilm matrix and is a heterogeneous mixture of various biomolecules such as polysaccharides, proteins, and extracellular DNA/RNA (eDNA/RNA). In addition, bacteriophage (phage) was also found to be an integral component of the matrix and can serve as a protective barrier. In recent years, the roles of proteins, polysaccharides, and phages in the virulence of biofilms have been well studied. However, a mechanistic understanding of the release of such biomolecules and their interactions with antimicrobials requires a thorough review. Therefore, this article critically reviews the various mechanisms of release of matrix polymers. In addition, this article also provides a contemporary understanding of interactions between various biomolecules to protect biofilms against antimicrobials. In summary, this article will provide a thorough understanding of the functions of various biofilm matrix molecules.<h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142434896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

N6-methyladenosine-modified lncRNA in Staphylococcus aureus-injured bovine mammary epithelial cells 金黄色葡萄球菌损伤的牛乳腺上皮细胞中的 N6-甲基腺苷修饰 lncRNA

IF 2.3 3区生物学

Archives of Microbiology Pub Date : 2024-10-12 DOI: 10.1007/s00203-024-04156-4

Haojun Xu, Xuan Wu, Zhiming Yang, Xinhuai Shi, Aizhen Guo, Changmin Hu

{"title":"N6-methyladenosine-modified lncRNA in Staphylococcus aureus-injured bovine mammary epithelial cells","authors":"Haojun Xu, Xuan Wu, Zhiming Yang, Xinhuai Shi, Aizhen Guo, Changmin Hu","doi":"10.1007/s00203-024-04156-4","DOIUrl":"10.1007/s00203-024-04156-4","url":null,"abstract":"<div>Staphylococcus aureus-induced mastitis is a serious disease in dairy bovine, with no currently effective treatment. Antibiotics demonstrate certain therapeutic potency in dairy husbandry; they generate drug-resistant bacteria, thereby harming public health. LncRNAs and m6A have been verified as potential targets in infectious diseases and have powerful regulatory capabilities. However, the biological regulation of lncRNAs with m6A modification in mastitis needs further investigation. This study aims to determine the m6A-modified lncRNAs in bovine mammary epithelial cells and their diversity during S. aureus induction. Heat-inactivated S. aureus was used to develop the cell injury model, and we subsequently found low cell viability and different m6A modification levels. Our analysis of m6A-modified lncRNA profiles through MeRIP-seq revealed significant differences in 140 peaks within 130 lncRNAs when cells were injured by S. aureus. Furthermore, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that these differential m6A-modified lncRNAs were mainly enriched in the WNT pathway, and their functions were associated with amino acid metabolism, lipid translocation, and metalloproteinase activity. Here, we report for the first time lncRNAs with m6A modification in regulating S. aureus infection, revealing potential mechanisms and targets of infectious diseases, such as mastitis, from an epigenetics perspective.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142411498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0