The ameliorative effect of Lactiplantibacillus plantarum SCS2 on DSS-induced murine colitis

IF 2.6 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-09-08 DOI:10.1007/s00203-025-04458-1

Qian Luo, Shukun Liu, Ying Zhang, Xiao Meng, Lishi Jiang

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Abstract

The inhibitory effects of Lactiplantibacillus plantarum on inflammatory responses are known, but its action mechanisms in oxidative stress, immunomodulation, and intestinal homeostasis remain of interest. Accordingly, we investigated the protective effects of Lactiplantibacillus plantarum SCS2 (L. plantarum SCS2) against sodium dextran sulfate (DSS)-induced colitis in mice as well as elucidated its impact on inflammation, oxidative stress, and intestinal microbiota. The gut microbiota composition was analyzed by high-throughput 16S rDNA sequencing, and the levels of inflammatory cytokines and oxidative stress indicators in the colon were determined by quantitative polymerase chain reaction and biochemical analysis. The results revealed that pretreatment with L. plantarum SCS2 (10 × 10⁸ CFU/mL) attenuated DSS-induced pathological conditions such as colon shortening and weight loss in mice (P < 0.05). Moreover, the expression of colonic inflammatory factors was reduced, and colonic oxidative stress was regulated. In addition, L. plantarum SCS2 increased the beneficial mouse intestinal flora, which promoted short-chain fatty acid production. Thus, L. plantarum SCS2 could alleviate colitis, showing potential as a novel dietary intervention agent.

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植物乳杆菌SCS2对dss诱导的小鼠结肠炎的改善作用

植物乳杆菌对炎症反应的抑制作用是已知的，但其在氧化应激、免疫调节和肠道稳态中的作用机制仍不清楚。因此，我们研究了植物乳杆菌SCS2 （L. plantarum SCS2）对右旋糖酐硫酸钠（DSS）诱导小鼠结肠炎的保护作用，并阐明了其对炎症、氧化应激和肠道微生物群的影响。通过高通量16S rDNA测序分析肠道菌群组成，通过定量聚合酶链反应和生化分析检测结肠炎症因子和氧化应激指标水平。结果显示，植物乳杆菌SCS2 （10 × 108 CFU/mL）预处理可减轻小鼠dss诱导的结肠缩短和体重减轻等病理症状（P < 0.05）。结肠炎性因子表达降低，结肠氧化应激受到调节。此外，植物乳杆菌SCS2增加了小鼠肠道有益菌群，促进了短链脂肪酸的产生。因此，植物乳杆菌SCS2可以缓解结肠炎，显示出作为一种新的饮食干预剂的潜力。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.