Archives of Microbiology最新文献

{"title":"Exploring biodegradable alternatives: microorganism-mediated plastic degradation and environmental policies for sustainable plastic management","authors":"Rita de Cássia Garcia Simão,&nbsp;Paula Maria Carneiro Rocha,&nbsp;Júlia Thays Kava Martins,&nbsp;Maurício Turkiewicz,&nbsp;Jacqueline Plewka,&nbsp;José Luis da-Conceição Silva,&nbsp;Alexandre Maller,&nbsp;Marina Kimiko Kadowaki,&nbsp;Álvaro Pereira Sampaio Costa-Júnior","doi":"10.1007/s00203-024-04170-6","DOIUrl":"10.1007/s00203-024-04170-6","url":null,"abstract":"<div><p>Plastics offer versatility, durability and low production costs, but they also pose environmental and health risks due to improper disposal, accumulation in water bodies, low recycling rates and temporal action that causes physicochemical changes in plastics and the release of toxic products to animal health and nature. Some microorganisms may play crucial roles in improving plastic waste management in the future. <i>Cunningamella echinulata</i> has been identified as a promising candidate that remains viable for long periods and produces a cutinase that is capable of degrading plastic. Other recent approaches involving the use of microorganisms are discussed in this review. However, there does not seem to be a single science that is efficient or most appropriate for solving the problem of plastic pollution on the planet at present. Regulations, especially the implementation of different laws that address the entire plastic cycle in different countries, such as Brazil, the USA, China and the European Union, play important roles in the management of this waste and can contribute to reducing this problem. In the context of the transversality of the information compiled here, the current limitations are discussed, and an effective plan is proposed to reduce plastic pollution. Although it is challenging, it involves implementing legislation, promoting sustainable alternatives, improving collection and recycling systems, encouraging reuse, supporting research and technological innovation, promoting corporate responsibility, collaborating globally, raising public awareness, optimizing waste management and, above all, continuously monitoring the progress of actions based on measurable metrics.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Insights into molecular mechanisms of phytochemicals in quorum sensing modulation for bacterial biofilm control","authors":"Anh Ngoc Xuan Nguyen,&nbsp;Krit Thirapanmethee,&nbsp;Teerawit Audshasai,&nbsp;Piyatip Khuntayaporn,&nbsp;Mullika Traidej Chomnawang","doi":"10.1007/s00203-024-04171-5","DOIUrl":"10.1007/s00203-024-04171-5","url":null,"abstract":"<div><p>Biofilm formation is a common mechanism by which bacteria undergo phenotypic changes to adapt to environmental stressors. The formation of biofilms has a detrimental impact in clinical settings by contributing to chronic infections and promoting antibiotic resistance. Delving into the molecular mechanisms, the quorum sensing (QS) system involves the release of chemical signals for bacterial cell-to-cell communication, which activates and regulates the expression of various genes and virulence factors, including those related to biofilm formation. Accordingly, the QS system becomes a potential target for combating biofilm-associated concerns. Natural products derived from plants have a long history of treating infectious diseases in humans due to their antimicrobial properties, making them valuable resources for screening anti-biofilm agents. This review aims to discover the mechanisms by which phytochemical agents inhibit QS, potentially offering promising new therapies for treating biofilm-associated infections. By targeting the QS system, these phytochemical agents can prevent bacterial aggregation and biofilm formation while also diminishing other bacterial virulence factors. Additionally, it is important to focus on the advancement of techniques and experiments to investigate their molecular mechanisms. A thorough understanding of these mechanisms may encourage further studies to evaluate the safety and efficacy of phytochemical agents used alone or in combination with other strategies.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug resistance and possible therapeutic options against influenza A virus infection over past years","authors":"Muhammad Asif Raza,&nbsp;Muhammad Awais Ashraf","doi":"10.1007/s00203-024-04181-3","DOIUrl":"10.1007/s00203-024-04181-3","url":null,"abstract":"<div><p>Influenza A virus infection, commonly known as the flu, has persisted in the community for centuries. Although we have yearly vaccinations to prevent seasonal flu, there remains a dire need for antiviral drugs to treat active infections. The constantly evolving genome of the influenza A virus limits the number of effective antiviral therapeutic options. Over time, antiviral drugs become inefficient due to the development of resistance, as seen with adamantanes, which are now largely ineffective against most circulating strains of the virus. Neuraminidase inhibitors have long been the drug of choice, but due to selection pressure, strains are becoming resistant to this class of drugs. Baloxavir marboxil, a drug with a novel mode of action, can be used against strains resistant to other classes of drugs but is still not available in many countries. Deep research into nanoparticles has shown they are effective as antiviral drugs, opening a new avenue of research to use them as antiviral agents with novel modes of action. As this deadly virus, which has killed millions of people in the past, continues to develop resistance, there is an urgent need for new therapeutic agents with novel modes of action to halt active infections in patients. This review article covers the available therapeutic antiviral drug options with different modes of action, their effectiveness, and resistance to various strains of influenza A virus.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142579395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of H2S and cysteine homeostasis disturbance on ciprofloxacin sensitivity of Escherichia coli in cystine-free and cystine-fed minimal medium","authors":"Galina Smirnova,&nbsp;Aleksey Tyulenev,&nbsp;Lyubov Sutormina,&nbsp;Tatyana Kalashnikova,&nbsp;Zoya Samoilova,&nbsp;Nadezda Muzyka,&nbsp;Vadim Ushakov,&nbsp;Oleg Oktyabrsky","doi":"10.1007/s00203-024-04185-z","DOIUrl":"10.1007/s00203-024-04185-z","url":null,"abstract":"<div><p>Endogenous H₂S has been proposed to be a universal defense mechanism against different antibiotics. Here, we studied the role of H₂S transiently generated during ciprofloxacin (CF) treatment in M9 minimal medium with sulfate or produced by <i>E. coli</i> when fed with cystine. The <i>cysM</i> and <i>mstA</i> mutants did not produce H₂S, while <i>gshA</i> generated more H₂S in response to ciprofloxacin in cystine-free media. All mutants showed a reduced ability to maintain cysteine homeostasis under these conditions. We found no relationship between H₂S generation, cysteine concentration and sensitivity to ciprofloxacin. Excess cysteine, which occurred during <i>E. coli</i> growth in cystine-fed media, triggered continuous H₂S production, accelerated glutathione synthesis and cysteine export. This was accompanied by a twofold increase in ciprofloxacin tolerance in all strains except <i>gshA</i>, whose sensitivity increased 5–8-fold at high CF doses, indicating the importance of GSH in restoring the intracellular redox situation during growth in cystine-fed media.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Regulation mechanism of nitrite degradation in Lactobacillus plantarum WU14 mediated by Fnr","authors":"Shaoxian Chen,&nbsp;Hao Zeng,&nbsp;Hulin Qiu,&nbsp;Aiguo Yin,&nbsp;Fengfei Shen,&nbsp;Ying Li,&nbsp;Yunyi Xiao,&nbsp;Jinping Hai,&nbsp;Bo Xu","doi":"10.1007/s00203-024-04183-1","DOIUrl":"10.1007/s00203-024-04183-1","url":null,"abstract":"<div><p>Fumarate and nitrate reduction regulatory protein (Fnr)—a global transcriptional regulator—can directly or indirectly regulate many genes in different metabolic pathways at the top of the bacterial transcription regulation network. The present study explored the regulatory mechanism of Fnr-mediated nitrite degradation in <i>Lactobacillus plantarum</i> WU14 through gene transcription and expression analysis of oxygen sensing and <i>nir</i> operon expression regulation by Fnr. The interaction and the mechanism of transcriptional regulation between Fnr and GlnR were also examined under nitrite stress. After Fnr and GlnR purification by glutathione S-transferase tags, they were successfully expressed in <i>Escherichia coli</i> by constructing an expression vector. The results of electrophoresis mobility shift assay and qRT-PCR indicated that Fnr specifically bound to the <i>PglnR</i> and <i>Pnir</i> promoters and regulated the expression of nitrite reductase (Nir) and GlnR. After 6–12 h of culture, the expressions of <i>fnr</i> and <i>nir</i> under anaerobic conditions were higher than under aerobic conditions; the expression of these two genes increased with sodium nitrite (NaNO₂) addition during aerobic culture. Overall, the present study indicated that Fnr not only directly participated in the expression of Nir and GlnR but also indirectly regulated the expression of Nir through GlnR regulation.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction to: Effective RNA isolation method for gram-positive and acid-fast bacteria: metamorphosed from conventional RNA isolation techniques","authors":"Jignasa H. Bera,&nbsp;Leyon Selvin Raj. A,&nbsp;Hemant Kumar,&nbsp;Nilesh Pandey,&nbsp;Dhara N. Patel","doi":"10.1007/s00203-024-04180-4","DOIUrl":"10.1007/s00203-024-04180-4","url":null,"abstract":"","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ajoene: a natural compound with enhanced antimycobacterial and antibiofilm properties mediated by efflux pump modulation and ROS generation against M. Smegmatis","authors":"Ashirbad Sarangi,&nbsp;Bhabani Shankar Das,&nbsp;Isha Pahuja,&nbsp;Suvendu Ojha,&nbsp;Vishal Singh,&nbsp;Sidhartha Giri,&nbsp;Ashima Bhaskar,&nbsp;Debapriya Bhattacharya","doi":"10.1007/s00203-024-04189-9","DOIUrl":"10.1007/s00203-024-04189-9","url":null,"abstract":"<div><p>Tuberculosis (TB) continues to be a primary worldwide health concern due to relatively ineffective treatments. The prolonged duration of conventional antibiotic therapy warrants innovative approaches to shorten treatment courses. In response to challenges, the study explores potential of Ajoene, a naturally occurring garlic extract-derived compound, for potential TB treatment. <i>Mycobacterium smegmatis</i> as a model organism for <i>M. tuberculosis</i> (<i>M. tb</i>) to investigate Ajoene’s efficiency. In vitro techniques like antimicrobial susceptibility, antibiofilm, EtBr accumulation assay, and ROS assay evaluate the potency of Ajoene and conventional TB drugs against <i>Mycobacterium smegmatis.</i> An in-silico study also investigated the interaction between Ajoene and quorum-sensing proteins, specifically <i>regX3</i>, MSMEG_5244, and MSMEG_3944, which are involved in biofilm formation and sliding activity. In vitro findings revealed that Ajoene exhibited significant antibacterial activity by inhibiting growth and showing bactericidal effects. It also demonstrated additive interactions with common antibiotics such as Isoniazid and Rifampicin. Furthermore, Ajoene demonstrated a comparative interaction with commonly used antibiotics, such as Isoniazid and Rifampicin, and reduced <i>M. smegmatis</i> motility, both alone and in combination with these antibiotics. In silico analysis shows that Ajoene exhibited a higher binding affinity with <i>regX3</i>, a protein orthologous to the <i>regX3</i> gene in <i>M.tb</i>. Ajoene also demonstrated consistent antibiofilm effects, particularly when combined synergistically with Isoniazid and Rifampicin. Mechanistic investigations demonstrated Ajoene’s potential to inhibit efflux pumps and promote ROS generation in bacteria, suggesting a potential direct killing mechanism. Collectively, the findings emphasize Ajoene’s effectiveness as a novel antimycobacterial and antibiofilm molecule for TB treatment.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142563461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initial pH determines the morphological characteristics and secondary metabolite production in Aspergillus terreus and Streptomyces rimosus cocultures","authors":"Tomasz Boruta,&nbsp;Martyna Foryś,&nbsp;Weronika Pawlikowska,&nbsp;Grzegorz Englart,&nbsp;Marcin Bizukojć","doi":"10.1007/s00203-024-04186-y","DOIUrl":"10.1007/s00203-024-04186-y","url":null,"abstract":"<div><p>The influence of the initial pH on the morphology and secondary metabolite production in cocultures and axenic cultures of <i>Aspergillus terreus</i> and <i>Streptomyces rimosus</i> was investigated. The detected secondary metabolites (6 of bacterial and 4 of fungal origin) were not found in the cultures initiated at pH values less than or equal to 4.0. The highest mean levels of oxytetracycline were recorded in <i>S. rimosus</i> axenic culture at pH 5.0. Initiating the axenic culture at pH 5.9 led to visibly lower product levels, yet the presence of <i>A. terreus</i> reduced the negative effect of non-optimal pH and led to higher oxytetracycline titer than in the corresponding <i>S. rimosus</i> axenic culture. The cocultivation initiated at pH 5.0 or 5.9 triggered the formation of oxidized rimocidin. The products of <i>A. terreus</i> were absent in the cocultures. At pH 4.0, the striking morphological differences between the coculture and the axenic cultures were recorded.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 12","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-024-04186-y.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142562601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The clinical praxis of bacteriocins as natural anti-microbial therapeutics","authors":"Safura Nisar,&nbsp;Abdul Haseeb Shah,&nbsp;Ruqeya Nazir","doi":"10.1007/s00203-024-04152-8","DOIUrl":"10.1007/s00203-024-04152-8","url":null,"abstract":"<div><p>In recent decades, the excessive use of antibiotics has resulted in a rise in antimicrobial drug resistance (ADR). Annually, a significant number of human lives are lost due to resistant infectious diseases, leading to around 700,000 deaths, and it is estimated that by 2050, there could be up to 10 million casualties. Apart from their possible application as preservatives in the food sector, bacteriocins are gaining acknowledgment as potential clinical treatments. Not only this, these antimicrobial peptides have revealed in modulating the host immune system producing anti-inflammatory and anti-modulatory responses. At the same time, due to the ever-increasing global threat of antibiotic resistance, bacteriocins have gained attraction among researchers due to their potential clinical applications. Bacteriocins as antimicrobial peptides, represent one of the most important natural defense mechanisms among bacterial species, particularly lactic acid bacteria (LAB), that can fight against infection-causing pathogens. In this review, we are highlighting the potential of bacteriocins as novel therapeutics for inhibiting a wide range of clinically relevant and multi-drug-resistant pathogens (MDR). We also highlight the effectiveness and potential applications of current bacteriocin treatments in combating antimicrobial resistance (AMR), thereby promoting human health.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-vitro antibacterial and antibiofilm activities and in-silico analysis of a potent cyclic peptide from a novel Streptomyces sp. strain RG-5 against antibiotic-resistant and biofilm-forming pathogenic bacteria","authors":"El-Hadj Driche,&nbsp;Boubekeur Badji,&nbsp;Florence Mathieu,&nbsp;Abdelghani Zitouni","doi":"10.1007/s00203-024-04174-2","DOIUrl":"10.1007/s00203-024-04174-2","url":null,"abstract":"<div><p>The proliferation of multidrug-resistant and biofilm-forming pathogenic bacteria poses a serious threat to public health. The limited effectiveness of current antibiotics motivates the search for new antibacterial compounds. In this study, a novel strain, RG-5, was isolated from desert soil. This strain exhibited potent antibacterial and antibiofilm properties against multidrug-resistant and biofilm-forming pathogenic bacteria. Through phenotypical characterizations, 16S rRNA gene sequence and phylogenetic analysis, the strain was identified as <i>Streptomyces</i> pratensis with 99.8% similarity. The active compound, RG5-1, was extracted, purified by reverse phase silica column HPLC, identified by ESI-MS spectrometry, and confirmed by ¹H and ¹³C NMR analysis as 2,5-Piperazinedione, 3,6-bis(2-methylpropyl), belonging to cyclic peptides. This compound showed interesting minimum inhibitory concentrations (MICs) of 04 to 15 µg/mL and minimum biofilm inhibitory concentrations (MBICs 50%) of ½ MIC against the tested bacteria. Its molecular mechanism of action was elucidated through a molecular docking study against five drug-protein targets. The results demonstrated that the compound RG5-1 has a strong affinity and interaction patterns with glucosamine-6-phosphate synthase at − 6.0 kcal/mol compared to reference inhibitor (− 5.4 kcal/mol), medium with penicillin-binding protein 1a (− 6.1 kcal/mol), and LasR regulator protein of quorum sensing (− 5.4 kcal/mol), confirming its antibacterial and antibiofilm activities. The compound exhibited minimal toxicity and favorable physicochemical and pharmacological properties. This is the first report that describes its production from <i>Streptomyces</i>, its activities against biofilm-forming and multidrug-resistant bacteria, and its mechanism of action. These findings indicate that 2,5-piperazinedione, 3,6-bis(2-methylpropyl) has the potential to be a promising lead compound in the treatment of antibiotic-resistant and biofilm-forming pathogens.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"206 11","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142540592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}