Archives of Microbiology最新文献

{"title":"Correction to: Diplotaxis erucoides-derived SynCom suppresses Alternaria blight in mustard by regulating ROS homeostasis and defense response","authors":"Chetana Aggarwal, Jogendra Singh, Ashish Kumar Gupta, Vijayata Singh, Muthukkaruppan Elakkya, Sonam Priyadarshani, Sangeeta Paul","doi":"10.1007/s00203-026-05207-8","DOIUrl":"10.1007/s00203-026-05207-8","url":null,"abstract":"","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149126328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Klebsiella pneumoniae from community-acquired infections and an urban river share antimicrobial resistance profiles and high-risk lineages but differ in virulence","authors":"Adriano Souza Santos Monteiro,&nbsp;João Ricardo Filho,&nbsp;Vívian Santos Galvão,&nbsp;Vanessa Tibolla Moretto,&nbsp;Ana Beatriz Purificação,&nbsp;Lee Senhorinha Almeida Andrade,&nbsp;Camila Maria Piñeiro Silva,&nbsp;Lúcio Macedo Barbosa,&nbsp;Luciano Kalabric Silva,&nbsp;Soraia Machado Cordeiro,&nbsp;Ronald Edward Blanton,&nbsp;Joice Neves Reis,&nbsp;Mitermayer Galvão Reis","doi":"10.1007/s00203-026-05239-0","DOIUrl":"10.1007/s00203-026-05239-0","url":null,"abstract":"<div><p><i>Klebsiella pneumoniae</i> poses a significant public health threat, but the relationship between community-acquired (CA) infections and environmental isolates remains poorly understood. Therefore, this study aimed to assess whether <i>K. pneumoniae</i> from CA infections share microbiological and molecular features with isolates recovered from an urban river in Salvador, Brazil. A total of 115 isolates, including 65 from CA infections and 50 from river water, were analyzed using antimicrobial susceptibility testing, β-lactamase and virulence gene detection, and multilocus sequence typing (MLST). Resistance rates were similar between groups, including multidrug resistance (24.6% vs. 22.0%; <i>p</i> &gt; 0.05) and carbapenem resistance (4.6% vs. 4.0%; <i>p</i> &gt; 0.05). β-lactamase gene detection was consistent with observed resistance patterns, with <i>bla</i>_CTX−M detected in 18.5% of CA isolates and 12.0% of river isolates, while <i>bla</i>_KPC−2 (3.1% vs. 2.0%) and <i>bla</i>_NDM−1 (1.5% vs. 2.0%) occurred at comparable frequencies. However, hypervirulent <i>K. pneumoniae</i> isolates were identified only among CA infections (4.6%). MLST revealed high clonal diversity, with 88 sequence types (STs), including 14 novel STs, with most STs being singletons. Four STs were shared between groups, including the high-risk lineages ST11 and ST307. These findings indicate that clinically relevant <i>K. pneumoniae</i> lineages, including high-risk, occur beyond healthcare settings, with urban aquatic environments serving as potential environmental reservoirs.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00203-026-05239-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phylogenomic analysis and characterization of a novel metagenome-assembled genome from the Bay of Bengal and description of Candidatus Niobacter visakhapatnamensis gen. nov., sp. nov","authors":"Naga Radha Srinivas Tanuku,&nbsp;Anil Kumar Pinnaka,&nbsp;Pradeep Kumar Patel,&nbsp;Hardik Chavda,&nbsp;Naveen Sagar Garbhapu,&nbsp;Satyanarayana Reddy Tadi,&nbsp;Ann Rose","doi":"10.1007/s00203-026-05238-1","DOIUrl":"10.1007/s00203-026-05238-1","url":null,"abstract":"<div><p>A metagenome-assembled genome (NIO.Bin9^T) belonging to the family <i>Nannocystaceae was</i> generated from a marine sample collected from the Bay of Bengal coastal water, Visakhapatnam, India. Phylogenetic analysis based on the 16 S rRNA gene placed NIO.Bin9^T within the family <i>Nannocystaceae</i>, showing the sequence similarity (92.4%) to <i>Pseudenhygromyxa salsuginis</i> SYR-2^T. Genome-wide comparisons revealed average amino acid identity (AAI) values ranging from 52.2% to 53.4% and average nucleotide identity (ANI) values between 75.8% and 75.9%, relative to closely related genera, including <i>Nannocystis</i>, <i>Pseudenhygromyxa</i>, <i>Plesiocystis</i>, and <i>Enhygromyxa</i>. Phylogenomic and 16 S rRNA gene-based analysis consistently supported the placement of NIO.Bin9^T within the <i>Nannocystaceae</i> but distinct from existing taxa. Comprises complete pathways for carbohydrate degradation, glycolysis, the TCA cycle, nitrogen assimilation, sulfur metabolism, and biosynthesis of vitamins and cofactors. In particular, 29 biosynthetic gene clusters and an expanded CAZyme repertoire indicated robust potential for secondary metabolite production and complex carbohydrate utilization. Distinctive characteristics, such as the Csa3-type CRISPR component, the rifampin-resistance gene (<i>arr</i>), and an enriched suite of flagellar chemotaxis genes, differentiate the lineage from closely related taxa. Genome-relatedness analyses showed that NIO.Bin9^T was distinct from members of genera of <i>Nanocystaceae</i>, with both AAI and ANI values falling below the established genus threshold (&lt; 65% AAI and &lt; 80–83% for ANI), supporting its designation as a novel genus and species, for which we propose the name “<i>Candidatus</i> Niobacter visakhapatnamensis” gen. nov., sp. nov.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocess optimization and functional properties of cucumber juice fermented with Lacticaseibacillus casei NCDC-17: from physicochemical changes to health -promoting-effects","authors":"Mandeep Kumar,&nbsp;Renuka Sharma,&nbsp;Jyoti Yadav,&nbsp;Chahat Bhatia,&nbsp;Namita Singh","doi":"10.1007/s00203-026-05233-6","DOIUrl":"10.1007/s00203-026-05233-6","url":null,"abstract":"<div><p>A vegetable-based probiotic drink provides an alternative to dairy products for delivering beneficial microorganisms. This study was conducted to develop a fermented probiotic drink using cucumber juice. The probiotic starter culture used was <i>Lacticaseibacillus casei</i> NCDC-17. The fermentation process was optimized to enhance antioxidant activity, total phenolic content, and microbial viability. A rotatable central composite design using response surface methodology was used to set up the experimental design. The independent variables for the optimization were incubation temperature (30–45 °C), pH (5–9), and inoculum size (2–5%). The optimal fermentation conditions were observed to be incubation temperature 37.5 °C, pH 7, and inoculum size 3.5%. The results showed that the pH, total soluble solids, total flavonoids, and total sugar content were decreased after the fermentation. It was observed that the total phenolic content, and antioxidant activity were increased to a significant level after the fermentation. Results revealed that the fermented drink inhibits the key enzymes (alpha-amylase, and alpha-glucosidase) related to diabetes. The volatile compounds were identified using Gas Chromatography-Mass Spectrometry. A total of 54 distinct volatile compounds were identified following the fermentation process, indicating significant biochemical transformation and metabolic activity of the microbial culture. Cell-free supernatant of the drink exhibited antimicrobial activity with the highest and lowest inhibition against <i>Escherichia coli</i> (14 mm), and <i>Staphylococcus aureus</i> (11 mm), respectively. The fermented sample was evaluated for its cytotoxic potential against MCF-7 human breast cancer cell lines. The fermented sample exhibited marked growth inhibitory effects, against MCF-7 cell lines. Sensory evaluation was performed using a 7-point hedonic scale to evaluate attributes such as appearance and colour, mouth feel, aroma and flavor, taste, and overall acceptability. Results showed that the probiotic drink was well accepted.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic, model-guided intensification of fuel ethanol production in yeast: proteome constraints, CRISPR rewiring, and high-gravity fermentations","authors":"Wania Azhar,&nbsp;Isha Nasar,&nbsp;Zakish Fatima,&nbsp;Alishba Shaukat,&nbsp;Tri Handayani Kurniati,&nbsp;Iram Hafiz,&nbsp;Mian Anjum Murtaza,&nbsp;Muhammad Irfan","doi":"10.1007/s00203-026-05217-6","DOIUrl":"10.1007/s00203-026-05217-6","url":null,"abstract":"<div><p>The production of fuel ethanol by <i>Saccharomyces cerevisiae</i> is the consequence of the coupling between proteome limitations, metabolism regulation and the final stress of high-gravity fermentation. Recent developments in enzyme constrained genome-scale models and dynamic flux simulations have increased the predictability of the overflow metabolism, pathway usage under stress, and pathways that are critical for growth and ethanol production. Concurrently, CRISPR based genome engineering has permitted the precise modification of the pathways for glycolysis, fermentation, stress tolerance, and pentose sugar metabolism through multiplex editing, promoter tuning, and genome-scale perturbations. Using computational design integrated with CRISPR engineering, machine-learning guided promoter selection, and adaptive laboratory evolution, yeast strains with more balanced metabolic flux and enhanced robustness and ethanol productivity under these challenging growth conditions were obtained. With further advancements in whole-cell model technology, more sophisticated CRISPR tools, synthetic regulatory circuits and AI-based automated engineering platforms, the engineering of robust yeast chassis with improved stability, stress tolerance and productivity for sustainable and large-scale bioethanol generation will be promoted.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gut microbiota signatures and machine learning-based candidate feature prioritization in advanced colorectal cancer","authors":"Na Luo,&nbsp;Hui Yan,&nbsp;Nan Wang,&nbsp;Weining Fan,&nbsp;Ping Chen","doi":"10.1007/s00203-026-05220-x","DOIUrl":"10.1007/s00203-026-05220-x","url":null,"abstract":"<div><p>This single-center, cross-sectional case-control study aimed to characterize the gut microbiome profiles of patients with advanced colorectal cancer (CRC), identify candidate microbial features, and explore how well these features distinguished advanced CRC cases from healthy controls within the study dataset. Fecal samples were collected from 72 treatment-naïve patients with advanced CRC and 61 healthy controls. Microbial community structure was profiled using high-throughput sequencing of the V3-V4 region of the 16 S rRNA gene. The analytical pipeline included α/β-diversity analysis, multilevel taxonomic analysis, LEfSe, and machine-learning algorithms, including random forest (RF), gradient boosting machine (GBM), and LASSO, to identify key Amplicon Sequence Variants (ASVs) and evaluate their ability to discriminate between the two groups. Results showed significantly reduced α-diversity and distinct β-diversity in the CRC group. Key SCFA-producing genera (<i>Faecalibacterium</i>, <i>Agathobacter</i>, <i>Roseburia</i>) were consistently depleted. The RF feature-prioritization model achieved an out-of-bag (OOB) accuracy of 0.850 and an OOB AUC of 0.899. Nested five-fold cross-validation based on the prioritized ASVs yielded AUCs of 0.891, 0.900, and 0.891 for RF, GBM, and LASSO, respectively. These findings show internally reproducible case–control discriminatory patterns within the present cohort and support further evaluation of the prioritized microbial features in independent, clinically representative populations.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13630989/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial activity of seawater-derived Bacillus subtilis strain SC2 and its bioactive fraction","authors":"Sanjay Chavan,&nbsp;Bhagvat Lad,&nbsp;Tukaram Kadam","doi":"10.1007/s00203-026-05243-4","DOIUrl":"10.1007/s00203-026-05243-4","url":null,"abstract":"<div><p>The increasing prevalence of multidrug-resistant (MDR) bacterial pathogens highlights the need to explore microorganisms from under-investigated environments as potential sources of antibacterial metabolites. In the present study, marine <i>Bacillus</i> isolates were recovered from seawater and screened for antibacterial activity against three MDR <i>Escherichia coli</i> isolates. Twenty isolates were obtained, of which eight exhibited antibacterial activity and were subjected to secondary screening following solvent extraction. Among the tested extracts, the chloroform extract of isolate MBCP-8 showed the strongest inhibitory activity against all three MDR <i>E. coli</i> strains. Based on 16 S rRNA gene sequencing and phylogenetic analysis, MBCP-8 was identified as <i>Bacillus subtilis</i> strain SC2 and deposited in GenBank under accession number PP854588. The active chloroform extract was further fractionated by silica-gel column chromatography, and Fraction 3 exhibited the highest antibacterial activity. Broth microdilution testing was subsequently performed to determine the minimum inhibitory concentration (MIC) of Fraction 3. UPLC–UV–QTOF–MS profiling revealed two prominent molecular features tentatively annotated as discodermolide and 2-undecyl-4(1 H)-quinolinone N-oxide based on mass spectral/database matching. However, these annotations require confirmation using authentic standards and orthogonal structural techniques, including high-resolution MS/MS and 1D/2D NMR spectroscopy, before definitive compound identification. Overall, the findings demonstrate the antibacterial potential of seawater-derived <i>B. subtilis</i> SC2 and provide a basis for further purification, structural validation, mechanistic investigation, and evaluation of its metabolites against MDR pathogens.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic profiling of biofilm-associated genes (bap, ompA, csuE, and blaPER-1) in Acinetobacter baumannii clinical isolates","authors":"Falah Salim Manhal","doi":"10.1007/s00203-026-05235-4","DOIUrl":"10.1007/s00203-026-05235-4","url":null,"abstract":"<div><p><i>Acinetobacter baumannii</i> is an opportunistic pathogen frequently implicated in hospital-acquired infections and multidrug resistance (MDR). Its ability to form biofilms on medical devices contributes to antimicrobial resistance and prolonged persistence in clinical settings. Characterizing the molecular basis of biofilm formation is essential for designing efficacious infection prevention and control programs A total of 120 <i>A. baumannii</i> isolates were recovered from multiple clinical sources in an Iraqi hospital setting from June 2025 to April 2026. The isolates were identified based on phenotypic characteristics and confirmed by a PCR assay targeting the intrinsic <i>bla</i>_{OXA−51−like} gene. Microtiter plate assay was performed to assess biofilm formation, whereas antimicrobial susceptibility testing (AST) was conducted using the disk diffusion method according to CLSI guidelines. Conventional and quantitative real-time PCR (RT-qPCR) tests were conducted to determine the presence and expression of biofilm-related genes (<i>bap</i>, <i>ompA</i>, <i>csuE</i>, and <i>blaPER-1</i>) under biofilm-promoting conditions. The prevalence of MDR isolates reached 82.5% with high resistance rates to imipenem (88%) and meropenem (85%) and meropenem (85%), while colistin retained the greatest in vitro activity, with 91% susceptibility. Strong and moderate biofilm formation was observed in 35.0% and 31.7% of isolates, respectively. The most frequently detected genes were ompA (91.7%) and csuE (83.3%), followed by <i>bap</i> (68.3%) and <i>blaPER</i>-1 (56.7%). Gene expression profiling exhibited a marked elevation of <i>bap</i> (5.6 ± 0.4-fold) and <i>ompA</i> (4.9 ± 0.6-fold) in strong biofilm producers. A positive association was recorded between carbapenem resistance and biofilm strength (<i>p</i> &lt; 0.05). The findings indicate that multidrug resistance and biofilm formation are closely associated characteristics in <i>A. baumannii</i>. Higher expression of the investigated genes was associated with stronger biofilm-forming phenotypes, underscoring the potential relevance of molecular surveillance and biofilm-control strategies in Iraqi healthcare settings.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and optimization of salt-alkali tolerant phosphate-solubilizing Enterobacter sp. AD8: applications in soybean growth promotion and saline-alkali soil remediation","authors":"Yiqing Chen,&nbsp;Shaoke Bai,&nbsp;Tong Zhang,&nbsp;Xiaolei Shi,&nbsp;Fumeng He,&nbsp;Xu Feng,&nbsp;Fenglan Li","doi":"10.1007/s00203-026-05215-8","DOIUrl":"10.1007/s00203-026-05215-8","url":null,"abstract":"<div><p>Soil salinization severely limits crop growth, nutrient availability and phosphorus use efficiency. Combined saline-alkali stress and phosphorus deficiency greatly restrict soybean productivity. Applying salt-alkali tolerant phosphate-solubilizing bacteria is an effective biological strategy to improve crop performance and soil fertility. In this study, 15 strains were isolated from local saline-alkali soil. After functional screening, <i>Enterobacter </i>sp. AD8 showed excellent phosphate-solubilizing (264.21 mg/L soluble P) and IAA-producing (58.09 µg/mL) capacities. Its optimal culture conditions were determined as 25 °C, pH 6 and 3% inoculum size via optimization tests. Pot experiments showed AD8 significantly improved soybean seedling growth, enhanced antioxidant enzyme activity, reduced MDA content, and increased osmotic regulators. It also lowered soil pH (from 7.98 to 7.75) and salinity (from 0.304% to 0.265%), increased available phosphorus by 20.97% and available potassium by 54.54%, and boosted phosphatase and cellulase activities by 59.63% and 32.07%, respectively. This multi-functional strain and its optimized conditions support microbial inoculant development, providing valuable resources for soybean production and ecological remediation of saline-alkali soils.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between a carbapenem post-prescription audit and gram-negative susceptibility: a 6-year time-series analysis","authors":"Fazlollah Keshavarzi,&nbsp;Sanjay Rampal,&nbsp;Chiu Wan Ng,&nbsp;Koo Koon Lim,&nbsp;Soo Nee Tang,&nbsp;Sasheela Sri La Sri Ponnampalavanar","doi":"10.1007/s00203-026-05154-4","DOIUrl":"10.1007/s00203-026-05154-4","url":null,"abstract":"<div><p>Antimicrobial stewardship programs (ASPs) are critical for combating antimicrobial resistance (AMR), yet their longitudinal ecological impacts remain debated. This study evaluated the association between a carbapenem post-prescription audit and the susceptibility of seven Gram-negative bacteria over 6 years. A retrospective interrupted time-series (ITS) study was conducted in a tertiary hospital, comparing pre-implementation (2015–2017) and post-implementation (2018–2020) phases. Autoregressive Integrated Moving Average (ARIMA) modeling was utilized to assess trend modifications and immediate level changes in pathogen susceptibility before and after audit enforcement. The audit was associated with pathogen-specific trend reversals. <i>Proteus mirabilis</i> imipenem susceptibility reversed from a significantly declining baseline to a positive recovery trend post-intervention (β = 9.465, <i>P</i> = 0.005). Conversely, <i>Klebsiella pneumoniae</i> and <i>Pseudomonas aeruginosa</i> experienced significant downward post-intervention trend reversals for targeted carbapenems despite rising baselines. Ecologically, immediate susceptibility gains in third-generation cephalosporins for <i>Acinetobacter baumannii</i> and <i>Escherichia coli</i> contrasted with significant collateral susceptibility declines for fluoroquinolones and β-lactam/ β-lactamase inhibitors, including a severe immediate drop in <i>P. mirabilis</i> susceptibility to amoxicillin-clavulanate (β = − 43.023, <i>P</i> = 0.024). While targeted carbapenem audits are temporally associated with the reversal of specific declining susceptibility trajectories, they may also coincide with the displacement of selective pressure onto broad-spectrum alternatives. Comprehensive ASPs must concurrently monitor total institutional antibiograms to identify and manage this potential ecological displacement.</p></div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"208 12","pages":""},"PeriodicalIF":3.4,"publicationDate":"2026-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149103327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}