Archives of Microbiology最新文献_第4页

Novel antimicrobial peptide HFIAP-1 mutant as a β-lactamase inhibitor against extended-spectrum β-lactamases of Escherichia coli: a comprehensive in-silico approach 新型抗菌肽HFIAP-1突变体作为抗大肠杆菌扩展谱β-内酰胺酶的β-内酰胺酶抑制剂：一种综合的计算机方法。

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-24 DOI: 10.1007/s00203-025-04483-0

Elizabeth Annie George, Aniket Naha, Sudha Ramaiah

{"title":"Novel antimicrobial peptide HFIAP-1 mutant as a β-lactamase inhibitor against extended-spectrum β-lactamases of Escherichia coli: a comprehensive in-silico approach","authors":"Elizabeth Annie George, Aniket Naha, Sudha Ramaiah","doi":"10.1007/s00203-025-04483-0","DOIUrl":"10.1007/s00203-025-04483-0","url":null,"abstract":"<div>Extended-spectrum β-lactamases in Escherichia coli poses a significant threat for clinicians in tertiary healthcare settings, rendering treatments ineffective with newer β-lactam-β-lactamase inhibitors combinations. To overcome this, the present study was conducted to potential β-lactamase inhibitors, from a library of antimicrobial peptide mutants with enhanced antibacterial potency (~ 7–16%) as compared to their parent peptides. The study screened five peptides and their mutants based on physicochemical, pharmaco-immunogenic properties through comprehensive knowledge-based and machine-learning algorithms. Molecular docking analyses revealed HFIAP-1_M5 (L33K-W7C-N34C) as the potential inhibitor candidate, that predicted to inhibit ~ 82% of all the studied ESβLs (Class A–D) targets as analysed from the intermolecular interaction profiling. HFIAP-1_M5 exhibited enhanced binding affinities (~ 0.2–12.0%) than the parent peptides upon forming hydrogen bonds, van-der Waals interactions and salt bridges with crucial residues concerning the catalytic domains of class A [InterPro ID: IPR045155], class B [InterPro ID: IPR001279], class C [InterPro ID: IPR001466] and class D [InterPro ID: IPR001460] of β-lactamases as defined in the InterPro database. All-atom molecular dynamics simulations, supported by principal component analysis and free energy landscape analysis, confirmed the stability of ESβLs-HFIAP-1_M5 showing stable backbone profiles with minimal residue-level fluctuations throughout the simulation timeframe. Binding free energy calculations along with the energy decomposition analysis further highlighted the key residue contributions to complex stabilization. The study holds promise in developing a combination therapy upon augmenting HFIAP-1_M5 with susceptible β-lactam antibiotics to enhance the therapeutic spectrum of treatment after further experimental validations.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145129852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomic and structural insights into cazymes & novel AA10 lytic polysaccharide mono-oxygenase from Serratia marcescens CRi_33 for efficient lignocellulosic biomass deconstruction 酶的基因组和结构见解&来自粘质沙雷氏菌CRi_33的新型AA10裂解多糖单加氧酶，用于有效的木质纤维素生物质解构

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-23 DOI: 10.1007/s00203-025-04478-x

Chetana Akhand, Rashi Bamrotwar, Sejal Bhairam, Riddhi Singh, Nishant A. Dafale

{"title":"Genomic and structural insights into cazymes & novel AA10 lytic polysaccharide mono-oxygenase from Serratia marcescens CRi_33 for efficient lignocellulosic biomass deconstruction","authors":"Chetana Akhand, Rashi Bamrotwar, Sejal Bhairam, Riddhi Singh, Nishant A. Dafale","doi":"10.1007/s00203-025-04478-x","DOIUrl":"10.1007/s00203-025-04478-x","url":null,"abstract":"<div>Lignocellulosic biomass (LCB) saccharification remains a key challenge for cost-effective value-added products due to structural intricacy. Lytic polysaccharide mono-oxygenase (LPMOs) catalyses the glycosidic bonds cleavage oxidatively in crystalline polysaccharides, facilitating synergistic action with glycoside hydrolases (GHs). While fungal and actinobacterial LPMOs are well studied, bacterial LPMOs, especially in Gram-negative Serratia, are underexplored. The study investigates Serratia marcescens CRi_33 for its LCB deconstruction potential through enzymatic and genomic analyses. The strain exhibited elevated β-glucuronidase activity of 0.54 U/mL, endo-1,4-β-xylanase of 0.41 U/mL, and arabinosidase of 0.48 U/mL, reflecting strong hemicellulolytic potential. CRi_33-derived enzyme concentrate with biotin and cellobiose supplementation, released 152.04 mg/g of reducing sugars from pre-treated wheat straw (WS). FeCl₃ supplementation further enhanced saccharification to 165.04 ± 3.44 mg/g, resulting in an 8.55% increase. Genome analysis revealed 239 CAZymes, including 120 GHs and 11 auxiliary activity enzymes like laccases (AA1), lignin peroxidases (AA2), and benzoquinone reductases (AA6). Notably, a unique AA10 family LPMO, SmrLPMO10A, possessing a GlcNAc-binding domain, was structurally characterized. Structural modeling confirmed a conserved histidine brace, and docking studies showed strong binding affinity of -5.1 kcal/mol to hemicellulosic sugars, particularly galactose and mannose. This suggests dual specificity for chitin and hemicellulose, which is limited and less explored previously in Serratia LPMOs. These findings fill a critical gap in bacterial LPMO knowledge and highlights S. marcescens CRi_33’s efficiency for sustainable waste valorization and biorefinery applications.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110654","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phenotypic, secondary metabolite, and genomic characterization of Bacillus siamensis B03 from brackish water with Anti‑Vibrio potential 具有抗弧菌潜能的苦咸水暹芽孢杆菌B03的表型、次生代谢物和基因组特征

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-23 DOI: 10.1007/s00203-025-04484-z

Co Thi Kim Nguyen, Phuc Giang Hong Lam, Ngoc Thi Thuy Dang, Suong Ngoc Le, Ty Viet Pham, Nam Vo, Hung Tan Dinh, Oanh Viet Kieu Nguyen, Hoang Duc Nguyen

{"title":"Phenotypic, secondary metabolite, and genomic characterization of Bacillus siamensis B03 from brackish water with Anti‑Vibrio potential","authors":"Co Thi Kim Nguyen, Phuc Giang Hong Lam, Ngoc Thi Thuy Dang, Suong Ngoc Le, Ty Viet Pham, Nam Vo, Hung Tan Dinh, Oanh Viet Kieu Nguyen, Hoang Duc Nguyen","doi":"10.1007/s00203-025-04484-z","DOIUrl":"10.1007/s00203-025-04484-z","url":null,"abstract":"<div>This study aimed to provide the first genomic, functional, and metabolic characterization of Bacillus siamensis B03 from Lang Co Bay, Vietnam, and to evaluate the hypothesis that it harbors unique metabolic traits and antimicrobial potential against Vibrio spp.. B03 showed no hemolytic activity, moderate biofilm formation, tolerance to 10% NaCl, and broad extracellular enzyme secretion. Ethyl acetate extracts from the cell-free supernatant exhibited in vitro antibacterial activity against five Vibrio species, with inhibition zones of 14.7–22.3 mm. Metabolomic analysis tentatively identified 20 compounds, mainly cyclic dipeptides, some previously reported to possess antimicrobial properties. Draft, plasmid-free genome (3,745,205 bp; 46.4% GC) encodes 3,561 proteins and 72 tRNAs, with genes mainly for amino acid and carbohydrate metabolism, and contains gene clusters for bacillaene, fengycin, difficidin, bacillibactin (100% similarity), and surfactin (78%). Pan-genome analysis of 24 B. siamensis genomes revealed 2,254 core genes, with B03 contributing 31 unique genes, including those encoding NAD-dependent malic enzyme and adenylation domain proteins. These findings suggest B. siamensis B03 as a potential control agent against Vibrio pathogens.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145110451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E2 ubiquitin-conjugating enzyme Ubc11 regulates Rst2 protein stability in the fission yeast Schizosaccharomyces pombe E2泛素偶联酶Ubc11调控裂糖酵母Rst2蛋白稳定性

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-22 DOI: 10.1007/s00203-025-04485-y

Ying Huang, Yasir Nawaz

{"title":"E2 ubiquitin-conjugating enzyme Ubc11 regulates Rst2 protein stability in the fission yeast Schizosaccharomyces pombe","authors":"Ying Huang, Yasir Nawaz","doi":"10.1007/s00203-025-04485-y","DOIUrl":"10.1007/s00203-025-04485-y","url":null,"abstract":"<div>In Schizosaccharomyces pombe, many transcription factors, including Rst2, remain poorly understood. Rst2 functions downstream of the cAMP-PKA pathway, but its protein stability and potential degradation via the ubiquitin-proteasome system, are not well characterized. This study investigates the -regulation of Rst2 in S. pombe, focusing on expression and degradation via the ubiquitin-proteasome system and E2 enzymes. Protein expression and stability were analyzed by western blotting to assess the impact of E2 mutations on Rst2 regulation. Rst2 transcription remained low at early time points but increased over fourfold by 24 h, remaining elevated through 48 h. Protein stability assays showed rapid Rst2 degradation under cycloheximide (CHX) treatment, while CHX and bortezomib (BZ) treatment preserved Rst2 protein levels, indicating the proteasome-dependent degradation of Rst2. To determine which E2 enzyme(s) are invovled in Rst2 degradation, we individually deleted all non-essential E2 genes and generated two point mutants ubc4-P61S and ubc11-P93L mutant and found that Rst2 is stabilized only in ubc11-P93L. This study shows the role of the ubiquitin-proteasome system and E2 enzyme Ubc11 in regulating Rst2 in S. pombe.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The AroC gene is required for pathogenicity and antibiotic sensitivity in Edwardsiella tarda strain ET13 迟发爱德华菌ET13的致病性和抗生素敏感性需要AroC基因

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-22 DOI: 10.1007/s00203-025-04482-1

Huda Ahmed Hassan, Yi Yang, Guoqiang Zhu

{"title":"The AroC gene is required for pathogenicity and antibiotic sensitivity in Edwardsiella tarda strain ET13","authors":"Huda Ahmed Hassan, Yi Yang, Guoqiang Zhu","doi":"10.1007/s00203-025-04482-1","DOIUrl":"10.1007/s00203-025-04482-1","url":null,"abstract":"<div>Edwardsiella tarda (E. tarda) is a significant enteric pathogen responsible for causing diarrhea, wound infections, and fatal septicemia in humans, as well as various aquatic animals. To investigate the role of the aroC gene in E. tarda’s pathogenicity and antibiotic sensitivity, the ΔaroC mutant and aroC+ complemented strains of ET13 were constructed. Our in vitro experimental data showed that the ET13ΔaroC mutant failed to grow in Defined Minimal Medium (DMM) broth without aromatic amino acids and exhibited significantly reduced growth compared to the wild-type ET13WT and complemented ET13aroC+ when aromatic amino acids were supplemented. This indicates that the aroC gene is essential for the growth of E. tarda in the DMM medium. Notably, the ET13ΔaroC mutant displayed significantly reduced adhesion, invasion, and intracellular replication in Caco-2 cells compared to the ET13WT and ET13aroC+. Furthermore, the ET13ΔaroC mutant exhibited a reduction in biofilm formation ability and increased sensitivity to antibiotics. The 50% lethal dose (LD50) of ET13ΔaroC increased to 4.8 × 104 CFU, representing a nearly 5-fold increase compared to ET13WT and ET13aroC+. In addition, ET13ΔaroC exhibited reduced colonization in the kidney and spleen of Zebrafish. Notably, the impaired virulence phenotypes of ET13ΔaroC were observed even in nutrient-replete conditions (e.g., LB medium), indicating that these defects are not solely attributed to aromatic amino acid auxotrophy and instead point to a role of aroC beyond supporting bacterial growth. Collectively, our data demonstrate that the aroC gene is required for key virulence-associated phenotypes (adhesion, invasion, intracellular replication, and biofilm formation) and modulates antibiotic sensitivity in E. tarda strain ET13.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A predictive model developed to classify Leishmania promastigotes at two distinct life stages using MALDI-TOF mass spectrometry 利用MALDI-TOF质谱法建立了一个预测模型，对利什曼原虫在两个不同的生命阶段进行分类

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-22 DOI: 10.1007/s00203-025-04424-x

Sebastian Cubides-Cely, Betsy Muñoz Serrano, Enrique Mejía-Ospino, Patricia Escobar

{"title":"A predictive model developed to classify Leishmania promastigotes at two distinct life stages using MALDI-TOF mass spectrometry","authors":"Sebastian Cubides-Cely, Betsy Muñoz Serrano, Enrique Mejía-Ospino, Patricia Escobar","doi":"10.1007/s00203-025-04424-x","DOIUrl":"10.1007/s00203-025-04424-x","url":null,"abstract":"<div>Investigating the molecular differences between procyclic (non-infective) and metacyclic (infective) promastigotes is essential for understanding the Leishmania life cycle in the sandfly vector and may aid in identifying molecular markers specific to these parasite stages. MALDI-TOF MS, a robust mass spectrometry technique, identifies protein profiles by measuring their mass-to-charge (m/z) ratios. Machine learning (ML) aids in analysing, interpreting, and classifying the complex spectral dataset obtained. This research aims to develop a predictive model to classify procyclic and metacyclic stages based on their protein profiles obtained from MALDI-TOF MS spectra. Promastigotes from the two clones of L. amazonensis, previously typed by molecular approach, were cultured and collected on days 3 and 7 of growth at 27 °C. Our data included at least 10 biological replicates, each in triplicate, for each L. amazonensis clone. They were labelled as Clone1LB3D, Clone1LB7D, Clone2LP3D, and Clone2LP7D. Three supervised classification tools were utilised: support vector machine (SVM), artificial neural networks (ANN), and random forest (RF). The implementation was carried out using Python version 3.12. The predictor variables correspond to the intensities of the spectral signals of the parasites in the m/z ratio range of 600 to 9500. The SVM classifier achieved 100% accuracy, while ANN and RF achieved 95% and 85%, respectively. A confusion matrix confirmed the complete accuracy of SVM across clones and stages. For model robustness, we recommend conducting external validation using independent datasets, including those from different L. amazonensis clones and related Leishmania species, growth phases, and sample preparation methods.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145100743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cloning, expression, purification, and mutant construction of the chitinase PbCHI2 gene from Photobacterium sp. 光杆菌几丁质酶PbCHI2基因的克隆、表达、纯化及突变体的构建

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-20 DOI: 10.1007/s00203-025-04474-1

Zhe Wang, Ziqi Zhao, Lin Pan, Yawen Zhang, Zhuo Guo, Weiye Yang, Liangshan Feng, Xiaohui Wang

{"title":"Cloning, expression, purification, and mutant construction of the chitinase PbCHI2 gene from Photobacterium sp.","authors":"Zhe Wang, Ziqi Zhao, Lin Pan, Yawen Zhang, Zhuo Guo, Weiye Yang, Liangshan Feng, Xiaohui Wang","doi":"10.1007/s00203-025-04474-1","DOIUrl":"10.1007/s00203-025-04474-1","url":null,"abstract":"<div>Chitin, the second most abundant biopolymer on Earth, exhibits broad application potential in medicine, cosmetics, and agriculture through its degradation products (chito-oligosaccharides). Here, we characterized a cold-adapted chitinase (PbCHI2) secreted by Photobacterium sp. LG-29 isolated from deep-sea sediments. The PbCHI2 gene was cloned into an expression vector and heterologously expressed in Escherichia coli BL21(DE3), followed by protein structure prediction and molecular docking to identify key residues for mutagenesis. The enzyme showed optimal activity at 35 °C while retaining functionality at 4 °C, confirming its cold-adaptation. Notably, it maintained > 60% activity at 60 °C (substrate: 4-MU-GlcNAc3) and > 70% residual activity after 3 h at 45 °C, demonstrating remarkable thermostability. PbCHI2 exhibited pH-dependent activity, with peak performance at pH 8.0 and > 70% activity at pH 11. Metal ion effects revealed Mn2+, Ca2+, and Mg2+ as activators, whereas Fe2+/3+, Zn2+, Cu2+, and Cd2+ acted as inhibitors. Surprisingly, urea, Sodium Dodecyl Sulfate (SDS), and Dithiothreitol (DTT) enhanced activity at specific concentrations. Site-directed mutagenesis within a 5Å radius of the active site yielded mutants with improved catalytic efficiency, validated by HPLC analysis of degradation products (primarily GlcNAc2 and GlcNAc), suggesting exochitinase and β-N-acetylglucosaminidase activities. Structural analysis classified PbCHI2 as a GH18 family chitinase, featuring a ChtBD3 domain and PKD domain(s). To our knowledge, this is the first comprehensive study on a chitinase from Photobacterium sp., providing insights into its cold-adaptation mechanism and laying a foundation for engineering industrially relevant chitinases.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synthesis of N-acyl-homoserine lactones analogs for improving the potential application of Acidithiobacillus thiooxidans in bioleaching n -酰基同丝氨酸内酯类似物的合成以提高酸性硫氧化硫杆菌在生物浸出中的应用潜力

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-20 DOI: 10.1007/s00203-025-04473-2

Deping Tang, Mengjiao Li, Wentao Song, Yanpeng Xi, Yali Liu, Yanyan Lin, Hao Feng, Aihong Mao

引用次数: 0

Type II toxin-antitoxin systems as stress-responsive survival circuits in archaea and bacteria II型毒素-抗毒素系统在古细菌和细菌中作为应激反应性生存回路

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-19 DOI: 10.1007/s00203-025-04467-0

Md Rasel Uddin, Saifullah Saifullah

{"title":"Type II toxin-antitoxin systems as stress-responsive survival circuits in archaea and bacteria","authors":"Md Rasel Uddin, Saifullah Saifullah","doi":"10.1007/s00203-025-04467-0","DOIUrl":"10.1007/s00203-025-04467-0","url":null,"abstract":"<div>Simple early lifeforms with relatively small genomes were evolved with certain genetic circuitry to better their stress-response mechanism which significantly enhances their survival during stress, hypothetically. In this review, we conducted a comprehensive investigation to identify survival-focused genetic circuitry in microorganisms, focusing on type II toxin-antitoxin (TA) systems, particularly sought after due to their ubiquitousness in nature, composed of two functionally coordinated genes: one that transiently inhibits reproduction during stress and another that represses this inhibition under normal conditions, while simultaneously promoting DNA repair under stress. Our comprehensive analysis of 22 type II TA systems reveals diverse roles, including dormancy induction, biofilm formation, pathogenicity and DNA repair. While canonical modules such as HigAB and RelBE are well-characterized, others like ParDE, Kid-Kis, and YafO-YafN remain understudied in the context of dormancy or biofilm involvement. Additionally, systems such as DarT-DarG, YafQ-DinJ and CcdB-CcdA have been implicated in DNA repair pathways, suggesting broader functional repertoires beyond growth inhibition. Phylogenetic analyses further reveal that TA systems such as VapC-VapB and MazF-MazE are widely distributed among bacteria, archaea, and cyanobacteria, including lineages thriving in extreme environments like deep-sea hydrothermal vents, which are considered potential sites for the emergence of early life. The presence of TA loci in ancient microorganisms like Methanocaldococcus jannaschii and Microcystis aeruginosa hints at their ancient origin and possible role in microbial survival on early Earth. This review synthesizes current knowledge on type II TA systems as stress-responsive survival circuits and highlights their significance in microbial ecology, evolution, and adaptation.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of RNA isolation techniques for enhanced dsRNA yield and purity in bacterial expression platforms 提高细菌表达平台上dsRNA产率和纯度的RNA分离技术评价

IF 2.6 3区生物学

Archives of Microbiology Pub Date : 2025-09-19 DOI: 10.1007/s00203-025-04472-3

V. Rakesh, Anupma Singh, Amalendu Ghosh

{"title":"Evaluation of RNA isolation techniques for enhanced dsRNA yield and purity in bacterial expression platforms","authors":"V. Rakesh, Anupma Singh, Amalendu Ghosh","doi":"10.1007/s00203-025-04472-3","DOIUrl":"10.1007/s00203-025-04472-3","url":null,"abstract":"<div>RNA interference (RNAi) in pest control is emerging as a targeted, eco-friendly alternative to chemical pesticides in agriculture. Efficient and cost-effective production of high-yield and high-purity dsRNA is crucial for the success of this approach. Escherichia coli HT115 (DE3), commonly employed for dsRNA synthesis, offers high transformation efficiency and genetic flexibility. However, the yield and quality of dsRNA are strongly influenced by the RNA isolation and purification protocols used. The present study compares six RNA isolation methods, viz. TRIzol-isopropanol, TRIzol-absolute ethanol, RNA-XPress-isopropanol, RNA-XPress-absolute ethanol, ethanol isolation, and extended ethanol precipitation using the E. coli-L4440 expression system. The TRIzol-absolute ethanol method yielded the highest total RNA concentration (5.27 mg/mL), followed by TRIzol-isopropanol (4.84 mg/mL). Although ethanol isolation (1.35 mg/mL) and extended ethanol precipitation (1.87 mg/mL) produced lower total RNA quantities, they demonstrated superior dsRNA recovery efficiencies up to 84.44%. The findings underscore the importance of selecting and optimizing RNA isolation protocols to maximize dsRNA yield and purity, which are crucial factors for advancing RNAi-based pest management strategies. The results offer practical guidance for scalable dsRNA production, contributing to the development of sustainable agricultural practices.</div>","PeriodicalId":8279,"journal":{"name":"Archives of Microbiology","volume":"207 11","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145079053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0