Exploring the antifungal activities of secondary metabolites of Trichoderma harzianum against the leaf spot pathogen of turmeric Colletotrichum gloeosporioides (Penz.) Penz. & Sacc.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-05-29 DOI:10.1007/s00203-025-04358-4

Ankita Ghosh, P Muthulakshmi, S Varanavasiappan, S Maruthasalam, V Rajasree

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引用次数: 0

Abstract

The present study aimed to evaluate the antifungal activity of Trichoderma harzianum against Colletotrichum gloeosporioides, the causal agent of turmeric leaf spot. The pathogen was isolated from symptomatic turmeric leaves and identified using morphological traits and amplification of the ITS region, followed by sequencing. Pathogenicity was confirmed via Koch's postulates. Various biocontrol agents were tested in vitro, and T. harzianum exhibited the highest inhibition (75.92%) in dual culture assays. Crude culture filtrates of T. harzianum also inhibited the pathogen in a seeded agar assay, showing 22.22% inhibition. GC-MS profiling was carried out to identify the bioactive compounds responsible using the culture filtrates of T. harzianum, the pathogen, and their interaction. A total of 129 metabolites were identified, including phenolic acids, fatty acids, esters, and phthalates with reported antimicrobial activity. The interaction revealed compounds common to both organisms, suggesting biochemical cross-talk. This study demonstrates the potential of T. harzianum as an effective biocontrol agent against C. gloeosporioides and highlights key antifungal metabolites involved. Further characterisation of these compounds may support the development of bioformulations for eco-friendly disease management.

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哈兹木霉次生代谢物对姜黄炭疽病菌的抑菌活性研究Penz。& Sacc。

本研究旨在评价哈兹木霉对姜黄叶斑病病原菌炭疽菌的抑菌活性。从有症状的姜黄叶片中分离到病原菌，利用形态学特征和ITS区扩增进行鉴定，并进行测序。致病性通过科赫的假设得到证实。对多种生物防治剂进行体外抑菌试验，结果表明，哈氏梭菌在双培养试验中抑菌率最高（75.92%）。在种子琼脂实验中，粗培养滤液对病原菌也有抑制作用，抑制率为22.22%。采用气相色谱-质谱分析方法，利用哈茨芽孢杆菌、病原菌及其相互作用的培养滤液，鉴定其生物活性成分。共鉴定出129种代谢物，包括具有抗菌活性的酚酸、脂肪酸、酯和邻苯二甲酸酯。这种相互作用揭示了两种生物共有的化合物，表明生物化学相互作用。本研究证明了哈兹芽孢杆菌作为一种有效的生物防治剂的潜力，并强调了关键的抗真菌代谢产物。这些化合物的进一步表征可能支持开发用于生态友好型疾病管理的生物制剂。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.