Genomic and transcriptomic insights into the cellulose-degrading mechanism of Bacillus subtilis DC-11 and its novel cellulose catabolic pathway.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Archives of Microbiology Pub Date : 2025-05-29 DOI:10.1007/s00203-025-04356-6

Chen Chen, Minqi Zhang, Yuanhao Zhang, Xueping Jiang, Jia Kong, Jieling Zhou, Gaiqun Huang, Ran Zhang, Hao Li, Zhongzheng Gui

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引用次数: 0

Abstract

The accumulation of silkworm excrement poses a significant environmental challenge, contributing to pollution and resource squandering. Unraveling the novel mechanism governing bacterial cellulose degradation represents a vital avenue for augmenting cellulose conversion efficiency. This study investigated the cultivation of Bacillus subtilis DC-11 with different carbon sources, utilizing transcriptome sequencing to identify metabolic pathways and differentially expressed genes (DEGs) closely related to cellulose degradation. Transcriptome analysis revealed 3,917 DEGs between the carboxymethyl cellulose sodium (CMC-Na) treatment group and the glucose-supplemented (GLU) control group. Compared to the control group, the CMC-Na treatment group exhibited upregulation of 942 genes, while 1,996 genes were downregulated. KEGG pathway analysis of DEGs indicated the involvement of that carbohydrate metabolism and phosphotransferase system (PTS) pathways in response to cellulose degradation. Real-time quantitative PCR validation confirmed that the expressions of key genes, namely ytoP, bglH, gmuD, licH, licC, ywbA, licA, gmuA, and gmuB, associated with the PTS pathway were consistent with the transcriptomics data. These results suggest that B. subtilis DC-11 degrades cellulose via carbohydrate metabolism and PTS pathways. This study offers new insights into the cellulose metabolism pathway of B. subtilis DC-11, providing both a theoretical basis and innovative strategies for the efficient degradation of cellulose.

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枯草芽孢杆菌DC-11的纤维素降解机制及其新的纤维素分解代谢途径的基因组学和转录组学研究。

家蚕粪便的积累对环境构成了重大挑战，造成了污染和资源浪费。揭示控制细菌纤维素降解的新机制是提高纤维素转化效率的重要途径。本研究研究了不同碳源对枯草芽孢杆菌DC-11的培养，利用转录组测序鉴定与纤维素降解密切相关的代谢途径和差异表达基因（DEGs）。转录组分析显示，羧甲基纤维素钠（CMC-Na）处理组与葡萄糖补充（GLU）对照组之间存在3917个DEGs。与对照组相比，CMC-Na处理组有942个基因上调，1996个基因下调。KEGG途径分析表明，纤维素降解过程中涉及碳水化合物代谢和磷酸转移酶系统（PTS）途径。实时定量PCR验证证实，与PTS通路相关的关键基因ytoP、bglH、gmuD、licH、licC、ywbA、licA、gmuA、gmuB的表达与转录组学数据一致。这些结果表明枯草芽孢杆菌DC-11通过碳水化合物代谢和PTS途径降解纤维素。本研究对枯草芽孢杆菌DC-11的纤维素代谢途径有了新的认识，为纤维素的高效降解提供了理论基础和创新策略。

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来源期刊

Archives of Microbiology 生物-微生物学

CiteScore

4.90

自引率

3.60%

发文量

601

审稿时长

3 months

期刊介绍： Research papers must make a significant and original contribution to microbiology and be of interest to a broad readership. The results of any experimental approach that meets these objectives are welcome, particularly biochemical, molecular genetic, physiological, and/or physical investigations into microbial cells and their interactions with their environments, including their eukaryotic hosts. Mini-reviews in areas of special topical interest and papers on medical microbiology, ecology and systematics, including description of novel taxa, are also published. Theoretical papers and those that report on the analysis or ''mining'' of data are acceptable in principle if new information, interpretations, or hypotheses emerge.