Annals of clinical and laboratory science最新文献

{"title":"Optimizing UroVysion Fluorescence <i>In-Situ</i> Hybridization Reflex Testing in Atypical Urothelial Cell Diagnosis: A Comprehensive Cytomorphological Analysis.","authors":"Moe Thuzar, Ashley Flowers, Hidehiro Takei","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Urinary cytology is a key diagnostic tool for evaluating suspected urinary tract carcinoma, primarily high-grade urothelial carcinoma. The Paris System for Reporting Urinary Cytology (TPS), introduced in 2016, aimed to standardize reporting, though challenges with subjectivity and variability in diagnosing Atypical Urothelial Cells (AUCs) persist.</p><p><strong>Methods: </strong>This retrospective study explored the correlation between cytomorphological features in \"atypical\" diagnosis and UroVysion fluorescence <i>in-situ</i> hybridization (U-FISH) results. We assessed 19 cytomorphologic parameters in 121 cases. Histologic diagnosis, clinical findings, and patient history were also documented.</p><p><strong>Results: </strong>Of 121 cases, 38 (31.4%) tested U-FISH positive. The sensitivity and specificity of U-FISH in detecting malignancy were 93.5% and 91.1%, respectively. Five cytologic parameters-high atypical cellularity, nuclear/cytoplasmic ratio ≥0.7, nuclear enlargement (≥2.5 times), multinucleation, and mitosis-were significantly associated with U-FISH positivity. No association was found between TPS AUC criteria and U-FISH positivity. The majority of combinations of these key parameters exhibited high PPVs (>65.0%), suggesting their potential for classifying cases without U-FISH.</p><p><strong>Conclusions: </strong>Cases exhibiting combinations of these five parameters or any four including mitosis demonstrated a 100% positive predictive value (PPV). However, subsets of cases with certain parameter combinations showed moderate PPVs, indicating the potential utility of reflex U-FISH testing.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"837-844"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cornus Officinalis Iridoid Glycosides Protect against Chronic Renal Failure in Rats by Activating the Nrf2-antioxidant Pathway.","authors":"Aiping Zhao, Yuliang Qiu, Jing Wu, Shangzheng Dai, Chu Lin, Wenjie Zhang, Pengfei Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the therapeutic effects of Cornus officinalis iridoid glycosides (CIG) on rats with chronic renal failure (CRF).</p><p><strong>Methods: </strong>CRF was induced in adult male Sprague Dawley rats by nephrectomy. The rats were randomly divided into six groups: sham, sham+high-dose CIG (120 mg/kg/d for 14 days), CRF, CRF+low-dose CIG (60 mg/kg/d for 14 days), CRF+high-dose CIG, and CRF+high-dose CIG+ML385 (an inhibitor of nuclear factor erythroid 2-related factor 2 (Nrf2), single administration at 30 mg/kg). The pathohistological changes in renal tissues were evaluated with histological staining. The biomarker levels of renal function, oxidative stress, inflammation, and apoptosis, as well as Nrf2 expression in renal tissues were determined.</p><p><strong>Results: </strong>The rats in the sham and sham+high-dose CIG groups showed normal renal tissues. The rats in the CRF group displayed severe renal tissue damage/fibrosis and elevated biomarkers of renal dysfunction, which were accompanied by decreased nuclear and cytoplasmic Nrf2 expression and elevated levels of oxidative stress, inflammatory, and apoptotic markers in renal tissues. CIG treatment of CRF rats attenuated renal tissue damage and fibrosis, with the high-dose drug showing more significant improvements. The high-dose CIG also restored renal function in CRF rats, upregulated Nrf2, and downregulated oxidative stress, inflammatory, and apoptotic markers in renal tissues. These effects of high-dose CIG on CRF rats were reversed by ML385.</p><p><strong>Conclusions: </strong>CIG activates the Nrf2-antioxidant pathway to ameliorate oxidative and inflammatory renal tissue damage and restore renal function in CRF rats.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"765-773"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tiam1 Mediated Enhancement of AKT/mTOR and ERK/STAT3 Signaling Promotes Proliferation, Invasion and Migration of Pancreatic Cancer.","authors":"Xingchao Song, Jiahua Zhou, Weibin Yang, Yamin Han","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the role of Tiam1 in proliferation, invasion, and migration of pancreatic cancer.</p><p><strong>Significance: </strong>Previous studies have shown that T-cell lymphoma invasion and metastasis-inducing factor 1 (Tiam1) is involved in multiple tumor progression. However, the role and molecular mechanism of this molecule in pancreatic cancer remain unclear. The aim of this study was to determine the expression level of Tiam1, investigate the underlying molecular mechanism of Tiam1 in pancreatic cancer, and provide reference for the diagnosis and treatment of pancreatic cancer.</p><p><strong>Methods: </strong>The expression levels of Tiam1 protein in pancreatic cancer tissues and normal pancreatic tissues were investigated using Western blot experiments. We use short interfering RNA creating Tiam1-silenced pancreatic cancer cells. Subsequently, Edu, colony formation, cck-8 cell viability assays, and Transwell migration and invasion assays were performed to explore the biological role of Tiam1 in pancreatic cancer cells. The possible molecular pathways of Tiam1 in pancreatic cancer cells were investigated using Western blot experiments. We use subcutaneous tumorigenesis experiments to explore the role of tiam1 in vivo experiments.</p><p><strong>Results: </strong>The results showed that the expression of Tiam1 in pancreatic cancer tissues was significantly higher than that in normal pancreatic tissues. The proliferation, invasion, and migration ability of Tiam1-silenced pancreatic cancer cells was significantly decreased. Tiam1 positively regulates AKT/mTOR and ERK/STAT3 cell signaling pathways. Down-regulation of tiam1 expression slowed the proliferation of subcutaneous tumors.</p><p><strong>Conclusions: </strong>High expression of Tiam1 in pancreatic cancer promotes pancreatic cancer progression and may be a potential biomarker and therapeutic target for poor prognosis.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"739-747"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of Analytical Performance and Diagnostic Effectiveness of Anti-CCP Antibody Based on the Light-Initiated Chemiluminescence Assay.","authors":"Shi-Zhen Guan, Hong-Jing Zhang, Shan-Shan Cheng, Yi Wang, Hong-Wei Yang, Hui-Qiang Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the analytical and diagnostic performance of anti-cyclic citrullinated peptide (anti-CCP) antibody measured by the light-initiated chemiluminescence assay (LICA).</p><p><strong>Methods: </strong>A total of 193 patients with rheumatoid arthritis (RA) and 211 controls were included. Serum anti-CCP levels were assessed by LICA and enzyme-linked immunosorbent assay (ELSIA). The analytical performance of LICA anti-CCP assay was evaluated according to the clinical guidelines. The diagnostic performance of LICA anti-CCP assay was evaluated by a series of indicators such as sensitivity, specificity, and area under the receiver operating characteristic curve (AUC) compared with ELISA. The dynamic consistency of the two methods was compared by patient follow-up.</p><p><strong>Results: </strong>The LICA anti-CCP assay showed coefficient of variation of 0.6% - 3.3% and linearity within 10-500 U/mL with a correlation coefficient of 0.991. The LICA and ELISA showed similar sensitivity (88.60% vs 92.94%) and specificity (97.60% vs 94.95%) on determination of anti-CCP. The AUCs of anti-CCP detected by LICA and ELISA were 0.971 and 0.955 (<i>P</i>=0.052), respectively. Follow-up showed that the changes of anti-CCP measured by the two methods were consistent.</p><p><strong>Conclusions: </strong>LICA anti-CCP assay has good analytical and diagnostic performance for RA, which is comparable to ELISA, and has dynamic consistency.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"860-866"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Idylla^TM System for Rapid Evaluation of <i>EGFR</i> Mutation Status in Formalin-Fixed and Paraffin-Embedded Samples of Non-Small Cell Lung Cancer.","authors":"Yun-Jian Xu, Hui Wang, Ting Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>In approximately 80% of the patients with advanced non-small cell lung cancer (NSCLC), the only samples available are cytological material or a limited amount of tissue specimens. Therefore, we aimed to explore the effect of the Idylla™ system for rapid evaluation of epidermal growth factor receptor (<i>EGFR</i>) mutation status in formalin-fixed and paraffin-embedded (FFPE) samples of NSCLC patients.</p><p><strong>Methods: </strong>A total of 221 archived FFPE NSCLC tissue specimens were included in this study. Idylla™ system and amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) were used to detect the <i>EGFR</i> mutation status. The Idylla™ system results and ARMS-PCR results were compared. When results were discordant, the Idylla™ system would be retested, and a third analysis method was performed to confirm test results when possible.</p><p><strong>Results: </strong>Among the 220 valid results of the Idylla™ system, the Idylla™ identified <i>EGFR</i> mutations in 109 cases with an incidence of mutation of 49.55%. The Idylla™ assay results were in complete agreement with the results of the reference methods for 207 cases, yielding an overall accordance of 94.09% (207/220, 95% CI, 90.11%-96.82%). Consequently, the overall concordance with routine reference methods, including further analysis, was found to be 96.81% (213/220, 95% CI: 93.28%-98.60%), with a negative percentage agreement of 97.3% (108/111, 95% CI: 91.72%-99.30%) and a positive percentage agreement of 96.33% (105/109, 95% CI: 90.32%-98.81%).</p><p><strong>Conclusion: </strong>The Idylla™ system is a rapid and effective method that enables sensitive and reliable detection of <i>EGFR</i> mutations in FFPE samples of NSCLC patients, with minimal molecular expertise or infrastructure.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 5","pages":"652-660"},"PeriodicalIF":1.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulation of microRNA-129-2 by Hepatitis B Virus Promotes Cell Proliferation in Hepatocellular Carcinoma.","authors":"Laipeng Liu, Pingfeng Zhang, Sheng Sun, Chuanpei Cao, Zhi Song","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>MicroRNA-192-2 has been shown to have a role in the early diagnosis of hepatocellular carcinoma (HCC), but the relationship between microRNA-192-2 and hepatitis B virus (HBV) in HCC patients remains unclear.</p><p><strong>Methods: </strong>Specimens were collected from 56 HCC patients diagnosed with HBV infection and 56 HCC patients without viral infection. HBV and miR-129-2 levels in HCC tissues, adjacent tissues, and cell lines were analyzed by RT‒PCR. miR-129-2 mimics were transfected to induce the overexpression of miR-129-2 and cell function was assessed by a wound healing test. Tumor formation experiments in nude mice were conducted to validate tumor proliferation.</p><p><strong>Results: </strong>In HCC patients, miR-192-2 was significantly downregulated in tumor tissues compared to adjacent tissues. Notably, miR-192-2 level was even lower in HCC patients with HBV-infection than those without the viral infection. Moreover, there was a negative correlation between miR-192-2 and HBV levels. Regardless of HBV infection, patients with low miR-192-2 levels had poorer prognosis than those with high miR-192-2 levels. HBV infection suppressed miR-192-2 expression in HCC cell lines. Furthermore, overexpression of miR-192-2 significantly inhibited cell proliferation both <i>in vitro</i> and <i>in vivo</i> in the HBV-infected group.</p><p><strong>Conclusion: </strong>Our study revealed that the expression of miR-192-2, a crucial tumor suppressor gene, was suppressed by the presence of HBV.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 5","pages":"643-651"},"PeriodicalIF":1.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Case of Hb Phnom Penh Showing Different HbA1c Levels Depending on the High-Performance Liquid Chromatography System.","authors":"Masayuki Tojikubo, Takafumi Uchimura, Hidekazu Tamai, Masafumi Koga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The case of a 49-year-old man with Hb Phnom Penh whose HbA1c levels varied depending on the high-performance liquid chromatography (HPLC) system is presented. After the results of a health checkup showed a high HbA1c level of 7.6% measured by HPLC method, another HbA1c test measured by immunoassay at a local clinic showed a level of 5.2%. Given the discrepancy in HbA1c levels between assay methods, he visited our clinic. His fasting plasma glucose level was 95 mg/dL, but an oral glucose tolerance test showed diabetes mellitus. The mean plasma glucose level was 117 mg/dL on intermittently scanned continuous glucose monitoring (isCGM), giving an estimated HbA1c level of 5.9%. Based on the results of isCGM, the present case was considered to be not diabetes mellitus. The same specimen was assayed for HbA1c by various methods (systems), including HPLC with Tosoh G9, HPLC with Arkray HA-8180 and HA-8180T, affinity assay, and enzymatic assay, giving HbA1c levels of 7.3%, 4.9%, 5.0%, 5.4%, and 5.3%, respectively. A globin gene analysis diagnosed Hb Phnom Penh (<i>α</i>1-117Phe-Ile-<i>α</i>1-118Thr). The present case had a unique hemoglobin variant, resulting in high HbA1c levels when measured with Tosoh's HPLC systems and low HbA1c levels with Arkray's HPLC systems.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 5","pages":"699-705"},"PeriodicalIF":1.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613770","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemolytic Transfusion Reactions Due to Le^a and Le^b Antibodies.","authors":"Hai-Yan Li, Li-Jun Li, Deng-Shan Yang, Xue-Jun Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A 20-year-old pregnant woman at 12 week gestation with a history of thalassemia was admitted to the hospital with Hb 60g/L. She received two transfusions of 2 units of negative crossmatched washed red blood cells (RBCs) each, but shortly after she experienced a transfusion reaction. Symptoms included chest tightness, dyspnea, chills, and soy sauce colored urine. A post-transfusion specimen was sent to the blood type reference laboratory (BTRL) for investigation, which revealed the presence of anti-Le^a and anti-Le^b antibodies causing the immediate acute hemolytic transfusion reaction; interestingly, the patient's Le^a antibody was found to be IgM, while the Le^b antibody was both IgM and IgG. This combination of antibodies is rare and highlights the potential for clinically insignificant Lewis cold antibodies to cause serious reactions. It is important to not overlook these antibodies and to select antigen-negative units rather than relying solely on blood crossmatching. The use of polybrene in crossmatching blood tests may have limitations in the presence of Lewis antibodies, so alternative methods should be considered in difficult cases to ensure safe and effective transfusions. This case emphasizes the need for thorough testing and careful selection of blood products to reduce the risk of transfusion reactions and improve overall transfusion safety.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 5","pages":"688-693"},"PeriodicalIF":1.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Osteoprotegerin (OPG)/Receptor Activator of Nuclear Factor-κB Ligand (RANKL)/Receptor Activator of Nuclear Factor-κB (RANK) on Aortic Valve Calcified.","authors":"Wei Luo, Jing Wang, Xia Yang, Junshan Li, Yanqiu Song, Hongliang Cong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of osteoprotegerin (OPG)/receptor activator of nuclear factor-[Formula: see text]B ligand (RANKL)/receptor activator of nuclear factor-[Formula: see text]B (RANK) nuclear factor on aortic valve calcification.</p><p><strong>Methods: </strong>The aortic valve tissue was collected from 132 aortic stenosis (AS) patients who underwent valve replacement. The valve tissue was stained with hematoxylin eosin (HE) and alizarin red calcium salt deposition. At the same time, CD68, RUNX2, TRAP immunohistochemical staining and double staining were performed.</p><p><strong>Results: </strong>ELISA analysis showed that the peripheral blood OPG value in the severe calcification group was significantly higher than mild calcification group (<i>P</i>=0.000) and non-calcification group (<i>P</i>=0.000), while the content of peripheral blood sRANKL in the severe calcification group was significantly lower than that in the non-calcification group (<i>P</i>=0.001). The valval OPG value in the mild calcification group was significantly higher than that of the non-calcification group (<i>P</i>=0.001) and the severe calcification group (<i>P</i>=0.040), and the valval sRANKL value of the severe calcification group was significantly lower than that of the non-calcification group (<i>P</i>=0.000).</p><p><strong>Conclusion: </strong>The expression of OPG and RANKL can regulate the inflammatory reaction of valve and the balance between bone formation and resorption, thus affecting the progress of valve calcification.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 5","pages":"633-642"},"PeriodicalIF":1.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concurrent High-Grade Serous Carcinoma with Mucinous Differentiation and Ectopic Complete Molar Pregnancy of the Fallopian Tube: A Case Report.","authors":"Flora Mae G Sta Ines, Bushra Al-Tarawneh, Mary Marchese, Corinne Jansen, Monique E De Paepe, C James Sung, Nina Tatevian, M Ruhul Quddus, Elizabeth Lokich, Shivali Marketkar","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>We report the first documented case of concurrent ectopic complete hydatidiform mole (CHM) and high-grade serous carcinoma (HGSC) of the fallopian tube, associated with unique histologic features and mutations in the HGSC.</p><p><strong>Case report: </strong>The patient presented with pelvic pain and vaginal bleeding. Laboratory examination revealed a positive urine pregnancy test and high serum beta-human chorionic gonadotropin (β-hCG). Transvaginal ultrasound demonstrated a left adnexal mass suspicious for ectopic pregnancy. Salpingectomy was performed, and the fallopian tube, noted to be ruptured with a visible ectopic pregnancy, demonstrated chorionic villi with diffuse hydropic enlargement and mild trophoblast hyperplasia. p57/Kip2 immunohistochemical staining (IHC) showed loss of expression in villous cytotrophoblasts and stromal cells, confirming CHM. An incidental 0.5 cm focus of HGSC was identified in the fallopian tube, associated with serous tubal intraepithelial carcinoma (STIC). The tumor exhibited solid, transitional cell carcinoma-like, and acinar patterns, with intraluminal mucin highlighted by Alcian blue and PAS-D stains. Patient underwent staging surgery which resulted in the finding of a 0.7 cm HGSC in the left ovary with morphology concordant to the tubal mass, except for a pseudo-endometrioid pattern in the ovary. Notably, the HGSC is positive (2+, 90%) for FOLR1 antigen and harbored a pathogenic mutation (p.R273H) in exon 8 of the TP53 gene.</p><p><strong>Conclusion: </strong>This report emphasizes the crucial role of meticulous sampling and histopathologic examination of the fallopian tube, including the fimbriae, in all salpingectomy specimens. Furthermore, the case highlights the broad spectrum of morphologies encountered in HGSC, including mucinous differentiation. HGSC should be in the differential diagnosis when encountering mucin-producing high-grade carcinoma.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 5","pages":"679-684"},"PeriodicalIF":1.1,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142613782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}