Annals of clinical and laboratory science最新文献_第7页

In Memoriam: Kilmer S. McCully, MD, PhD (1933-2025). 纪念：基尔默·s·麦卡利，医学博士（1933-2025）。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Charles D Hawker, Roger L Bertholf

引用次数: 0

Effect of Butyric Acid on Apoptosis of Pancreatic β Cells. 丁酸对胰腺β细胞凋亡的影响。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Gang Wang, Xue Zhao, Guangyu He, Zhuo Li, Guixia Wang, You Lv

{"title":"Effect of Butyric Acid on Apoptosis of Pancreatic β Cells.","authors":"Gang Wang, Xue Zhao, Guangyu He, Zhuo Li, Guixia Wang, You Lv","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Islet β-cell dysfunction and insulin resistance are associated with obesity-associated type 2 diabetes mellitus. Short-chain fatty acids (SCFAs) are important regulatory factors for energy metabolism. We aimed to determine the effects of butyric acid (BA) on β-cell apoptosis in type 2 diabetes.Methods: A db/db transgenic mouse model and lipopolysaccharide (LPS)-stimulated β-cell model were used to determine the effects of BA on β-cells. The viability and apoptosis of β cells were determined using the MTT assay and flow cytometry, respectively. The expression of inflammatory factors IL-1β, TNF-α, IL-6, and IL-10 was measured using quantitative PCR. Activation of the NF-κB signaling pathway was detected by quantitative PCR and Western blotting. Fasting plasma glucose (FPG), fasting insulin (FINS), total cholesterol (TC), and triglyceride (TG) levels were assessed to evaluate glycolipid metabolism. Intestinal flora and SCFA analyses were performed to explore the changes in the gut microbiota.Results: Butyric acid treatment notably suppressed LPS-induced expression of pro-inflammatory cytokines and improved β-cell apoptosis. The levels of TC, TG, FPG, and FINS were significantly elevated in diabetic mice and decreased after BA treatment. The expression of the β-cell regulatory genes Maf-A and Foxo1 was notably decreased in diabetes, and BA treatment restored their expression levels.Conclusions: Butyric acid notably alleviated β-cell apoptosis and the secretion of inflammatory cytokines in diabetes by suppressing NF-κB signaling. Butyric acid improves the intestinal flora of diabetic mice, suggesting its potential as a novel therapeutic agent for diabetes.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"259-267"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technical Note: Experience of Implementing Tacrolimus Immunoassay on the Alinity i Analyzer in an Academic Medical Center: Small Percentage of Specimens Were Sent to Reference Laboratory for LC-MS/MS Analysis. 技术说明：某学术医疗中心在Alinity i分析仪上实施他克莫司免疫测定的经验：一小部分标本被送到参比实验室进行LC-MS/MS分析。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Melody Nelson, Tracy Kisler, Kelsey Woodard, Amitava Dasgupta

{"title":"Technical Note: Experience of Implementing Tacrolimus Immunoassay on the Alinity i Analyzer in an Academic Medical Center: Small Percentage of Specimens Were Sent to Reference Laboratory for LC-MS/MS Analysis.","authors":"Melody Nelson, Tracy Kisler, Kelsey Woodard, Amitava Dasgupta","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Liquid chromatography combined with tandem mass spectrometry (LC-MS/MS) is considered the gold standard for analysis of tacrolimus as this method is free from metabolite interferences. Due to complexity of analysis and initial high cost, many hospital laboratories use immunoassays for routine monitoring of tacrolimus. In 2023, we implemented a Federal Food and Drug Administration (FDA) approved Chemiluminescent microparticle immunoassay (CMIA) of tacrolimus on the Alinity i analyzer with an option for physicians to request LC-MS/MS analysis if they were not satisfied with the immunoassay result.Methods: CMIA tacrolimus was performed on the Alinity i analyzer, and the result was reported within four hours. After evaluating a particular tacrolimus result, if a physician requested LC-MS/MS analysis, the same whole blood specimen was sent to the reference laboratory. Then, we compared LC-MS/MS value with initial immunoassay result.Results: In a 12-month period, 25,897 tacrolimus tests were ordered by physicians. Only 388 specimens (1.49%) were sent to the reference laboratory for LC-MS/MS analysis. The overall correlation between LC-MS/MS value (x-axis) and immunoassay result was excellent (y=1.045x+0.652, r=0.96, n=388). Out of 388 specimens, only 70 specimens were considered discordant due to more than 20% bias between the LC-MS/MS and immunoassay values. When these specimens were compared, the regression equation was 1.277x+0.412, r=0.95.Conclusions: The CMIA assay on the Alinity i analyzer is suitable for routine therapeutic drug monitoring of tacrolimus with high physician satisfaction.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"268-271"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Grading T Lymphocyte-Mediated Acute Interstitial Nephritis Following Checkpoint Inhibitor Therapy. 检查点抑制剂治疗后T淋巴细胞介导的急性间质性肾炎分级。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Yazan A Al-Othman, Brandon D Metcalf, Olaf Kroneman, Jeffery M Gold, Sami Zarouk, Wei Li, Hassan D Kanaan, Ping L Zhang

{"title":"Grading T Lymphocyte-Mediated Acute Interstitial Nephritis Following Checkpoint Inhibitor Therapy.","authors":"Yazan A Al-Othman, Brandon D Metcalf, Olaf Kroneman, Jeffery M Gold, Sami Zarouk, Wei Li, Hassan D Kanaan, Ping L Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Immuno-checkpoint inhibitors (CPIs) such as PD-1/PD-L1 inhibitors have shown positive effects in treating various metastatic carcinomas but can cause complications like kidney dysfunction. This study aimed to determine if T lymphocytes were the dominant inflammatory cells in CPI-associated acute interstitial nephritis (AIN), graded using a modified Banff criteria for renal transplant cellular rejection.Methods: 20 renal biopsies from 18 patients with acute kidney injury following CPI treatment for metastatic carcinomas were evaluated. Infiltrating lymphocytes were stained for CD3 (T lymphocytes) and CD20 (B lymphocytes). AIN was graded using a modified Banff criteria for borderline changes and acute cellular rejection (ACR).Results: In 14 biopsies, typical AIN was dominated by CD3-positive T lymphocytes and a small percentage of B lymphocytes, with minimal eosinophils or plasma cells. There were one grade 3 AIN, four grade 2 AIN, and nine grade 1 AIN cases. Six biopsies without AIN showed either chronic thrombotic microangiopathy (TMA, n=1) or acute tubular injury (ATN, n=5). Following renal biopsies diagnosing AIN, nine out of 14 patients (64.3 %) experienced clinical improvement with steroid treatment.Conclusion: This study indicates that nephrotoxicity due to CPI treatment is characterized by T lymphocyte-mediated AIN, with the majority being grade 1 AIN according to the modified Banff criteria. Most patients showed some renal functional recovery in response to steroid treatment.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"172-178"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bone Marrow Stromal Cells Promote Osteogenic Differentiation and Induce Bone Remodeling and Healing in Osteoporosis. 骨髓基质细胞促进骨质疏松症的成骨分化，诱导骨重塑和愈合。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-03-01

Xiaowei Jiang, Qiang Wang, Pu Ying, Wentao Jiang, Zhiyuan Qian, Wei Lu

{"title":"Bone Marrow Stromal Cells Promote Osteogenic Differentiation and Induce Bone Remodeling and Healing in Osteoporosis.","authors":"Xiaowei Jiang, Qiang Wang, Pu Ying, Wentao Jiang, Zhiyuan Qian, Wei Lu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Osteoporosis is a common disorder marked by bone loss, fragility, and fractures. microRNAs (miRNAs) are highlighted as potential regulators of osteoblast differentiation, and exosomes carrying miRNAs from bone marrow stromal cells (BMSCs) are applied to treatment. Herein, we aimed to clarify the role of miR-17 and BMSCs in ovariectomized rats with osteoporosis.Methods: After establishment of an osteoporosis animal model, miR-17 and BARX2 expression in ovariectomized rat tissues and osteoblasts were detected. Osteoblasts were transfected with mimics and inhibitor and co-cultured with BMSCs, whilst cell proliferation and differentiation were evaluated.Results: miR-17 was poorly expressed in ovariectomized rat tissue, while BARX2 was up-regulated. Overexpression of miR-17 induced decreased BARX2 expression but enhanced proliferation and differentiation of osteoblasts in osteoporotic rats. Importantly, the presence of BMSCs also facilitated osteoblast differentiation and decreased BARX2 expression, which was abolished by addition of exosome inhibitors. Mechanistically, miR-17 targeted BARX2 expression to up-regulate ALP and Co1-I in osteoporosis.Conclusion: Collectively, miR-17 delivered by BMSC exosomes promotes osteogenic differentiation and induces bone healing in osteoporosis through BARX2 regulation. The combined use of exosome inhibitors confirmed the therapeutic effect of BMSCs and BMSC exosomes containing miRNAs on various diseases.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"231-239"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Technical Note: Discordance Between Point of Care (POCT) BNP and Laboratory-Based NT-proBNP Values at Decision Cut-off in Some Patients - POCT BNP Values Below Cut-off Should be Interpreted with Caution. 技术说明：在一些患者中，护理点（POCT） BNP值与基于实验室的NT-proBNP值在决策临界值处不一致- POCT BNP值低于临界值应谨慎解释。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Megan Nelson, Kristine Zinn, Mercedes Boyd, Amitava Dasgupta

{"title":"Technical Note: Discordance Between Point of Care (POCT) BNP and Laboratory-Based NT-proBNP Values at Decision Cut-off in Some Patients - POCT BNP Values Below Cut-off Should be Interpreted with Caution.","authors":"Megan Nelson, Kristine Zinn, Mercedes Boyd, Amitava Dasgupta","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Both B-type natriuretic peptide (BNP) and NT-pro BNP are markers for heart failure. In our hospital and other institutions, both point of care (POCT) BNP and clinical laboratory-based NT-Pro BNP test are used interchangeably by clinicians. We evaluated any discrepancy between these tests at decision cut-off point.Method: In a three-month period, in 120 patients, both POCT BNP and NT-proBNP were ordered at the same time and we evaluated these results.Results: We observed significant discordant results in 14 of these patients where POCT BNP concentrations were normal, but NT-pro BNP concentrations were elevated. Moreover, correlation between POCT BNP and NT-proBNP concentrations was also poor and in some cases NT-proBNP values were 15-fold higher than BNP.Conclusions: Because of this significant discordance, if both tests are ordered for initial diagnosis, POCT BNP result should be interpreted with caution if below the cut-off. Moreover, if only POCT BNP is ordered it may be normal in some patients with heart failure.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"142-144"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IL-10 Cytokine Responses in Chlamydia pneumoniae-Stimulated Human Peripheral Blood Mononuclear Cells. IL-10细胞因子在肺炎衣原体刺激的人外周血单核细胞中的反应。

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Tamar A Smith-Norowitz, Sarah Shidid, Nutchaya Amornruk, Haram Abdelmajid, Wefag Ahmed, Yitzchok M Norowitz, Stephan Kohlhoff

{"title":"IL-10 Cytokine Responses in Chlamydia pneumoniae-Stimulated Human Peripheral Blood Mononuclear Cells.","authors":"Tamar A Smith-Norowitz, Sarah Shidid, Nutchaya Amornruk, Haram Abdelmajid, Wefag Ahmed, Yitzchok M Norowitz, Stephan Kohlhoff","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Chlamydia pneumoniae (C. pneumoniae) is a gram-negative intracellular bacterium that causes respiratory infections and may contribute to inflammatory responses in asthma. Cell-mediated immune responses are important for protective immunity against C. pneumoniae; however, these responses may be impaired in asthma. Interleukin (IL)-10 is a cytokine that modulates innate and adaptive immunity. IL-10 is necessary for resolution of acute infection, and maturation of memory T cells. The aim of this study sought to compare IL-10 responses between subjects with or without asthma, and according to C. pneumoniae IgG antibody (Ab) or serum IgE status.Methods: Peripheral blood mononuclear cells (PBMC) (1×106/mL) from stable adult asthmatic (N=6) and non-asthmatic subjects (N=6) were infected +/- C. pneumoniae TW-183 at a multiplicity of infection (MOI)=0.1, using dose responses (1:10, 1:100), and cultured 48 hrs. IL-10 responses were measured in supernatants (ELISA). C. pneumoniae-IgG Abs and total serum IgE levels were measured in serum (EIA, ELISA).Results: Cytokine responses (mean differences: unstimulated-stimulated cells) were significant for IL-10 (1:10, 1:100) (-618±443, -1405±155) (P=0.0005, 0.0005) in all subjects (Wilcoxon signed-rank test). When subjects were stratified according to asthma, C. pneumoniae IgG Ab or serum IgE status, no significant cytokine responses were observed.Conclusions: C. pneumoniae stimulates IL-10 responses in subjects with or without asthma. These responses are not dependent on either asthma, C. pneumoniae IgG Ab or serum IgE status.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"79-83"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of miR-190a-3P on Chronic Diabetic Nephropathy by Regulating FGFR3. miR-190a-3P通过调节FGFR3对慢性糖尿病肾病的影响

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Yuan Lin, Chaoyan Yin, Yanjing Liu, Donghui Lu, Xiaofen Lian

{"title":"Effect of miR-190a-3P on Chronic Diabetic Nephropathy by Regulating FGFR3.","authors":"Yuan Lin, Chaoyan Yin, Yanjing Liu, Donghui Lu, Xiaofen Lian","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Chronic diabetic nephropathy (CDN) is one of the common complications of the chronic diabetes. The FGF23/FGFR3-mediated signaling pathway is involved in CDN. Whether miR-190a-3P participates in CDN through regulation of FGFR3 remains to be elucidated. The present study evaluated miR-190a-3P's effect on CDN.Methods: Mice were divided into a control group (NC group), a CDN group, and CDN+miR-190a-3P antagonist group, followed by analysis of miR-190a-3P and FGFR3 level by qRT-PCR, FGFR3, and Tubulin protein level by Western blot as well as blood glucose, serum creatinine (Cr), and urea nitrogen (BUN) using an automatic biochemical analyzer.Results: Compared with the NC group, the CDN group had a significantly higher miR-190a-3P level in kidney tissue, while the FGFR3 mRNA level was lower (P<0.01). FGFR3 was a target gene of miR-190a-3P. miR-190a-3P levels in the CDN+miR-190a-3P antagonist group were significantly reduced compared with the CDN group (P<0.05). Meanwhile, FGFR3 protein levels in miR-190a-3P antagonist group were significantly increased (P<0.05). Compared with the NC group, the CDN group showed significantly reduced blood glucose level and elevated BUN and Cr level (P<0.01). However, CDN+miR-190a-3P antagonist group showed significantly increased blood glucose and reduced BUN and Cr level compared with CDN group (P<0.05).Conclusion: miR-190a-3P can directly bind to the 3'-UTR of FGFR3 mRNA and reduce FGFR3 protein levels, contributing to the occurrence of CDN.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"48-53"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effect of Jiawei Shuxin Tang on Myocardial Ischemia-Reperfusion in Rats with Qi Deficiency and Blood Stasis and Its Correlation with NRG-1. 加味舒心汤对气虚血瘀大鼠心肌缺血再灌注的保护作用及其与NRG-1的相关性

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Mingming Xu, Shen Wang, Dongming Lin

{"title":"Protective Effect of Jiawei Shuxin Tang on Myocardial Ischemia-Reperfusion in Rats with Qi Deficiency and Blood Stasis and Its Correlation with NRG-1.","authors":"Mingming Xu, Shen Wang, Dongming Lin","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To investigate the protective effect of Jiawei Shuxin Tang (JWSXT) on myocardial ischemia-reperfusion injury (MIRI) rats with Qi deficiency and blood stasis and its correlation with neuregulin-1 (NRG-1).Methods: 30 rats were used to establish the model of Qi deficiency and blood stasis, and were randomly divided into control group, MIRI model group, and JWSXT group. The traditional Chinese medicine (TCM) phenotypes, echocardiographic performance, myocardial reperfusion area, myocardial infarction area, HE staining, and NRG-1 content changes of rats in each group were compared one week after treatment.Results: We found that compared with the model group, rats in the JWSXT group showed increased activity as well as improved tongue texture and lip color (P<0.05); LVEF and LVFS increased in the JWSXT group (P<0.05; P<0.05). The percentage of infarct area and the percentage of myocardial ischemia reperfusion area in the JWSXT group was significantly reduced (P<0.05; P<0.05). Compared with the control group, the remaining NRG-1 content in myocardial tissues of rats in the two groups was decreased (P<0.01), and NRG-1 content in the JWSXT group was increased (P<0.05) compared with the model group.Conclusions: JWSXT can improve reperfusion injury in rats with Qi deficiency and blood stasis infarction, which correlates with NRG-1.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"84-95"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Consistency of Plasma Catecholamines and Metanephrines Analysis in China: A Comparison of Three Mainstream Commercially Available Liquid Chromatography Tandem Mass Spectrometry Kit Methods. 中国血浆儿茶酚胺和肾上腺素分析的一致性：三种主流市售液相色谱串联质谱试剂盒方法的比较

IF 1.1 4区医学

Annals of clinical and laboratory science Pub Date : 2025-01-01

Nijiao Qin, Qingxiang Liu, Zhenni Liu, Ziyang Li, Binghui Li, Zhe Guo, Zhiyang Chen, Weiming Wu, Min Hu, Qichen Long

{"title":"The Consistency of Plasma Catecholamines and Metanephrines Analysis in China: A Comparison of Three Mainstream Commercially Available Liquid Chromatography Tandem Mass Spectrometry Kit Methods.","authors":"Nijiao Qin, Qingxiang Liu, Zhenni Liu, Ziyang Li, Binghui Li, Zhe Guo, Zhiyang Chen, Weiming Wu, Min Hu, Qichen Long","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The consistency of plasma catecholamines and metanephrines measurements is crucial for the screening and diagnosis in pheochromocytomas and paragangliomas (PPGLs). There are many commercially available kits for plasma catecholamines and metanephrines testing, but their performance and consistency information are unknown. This study evaluated the performance of three mainstream commercially available LC-MS/MS kit methods and evaluated their consistency.Methods: Performance validation of three kit methods was performed according to Clinical Laboratory Standards Institute (CLSI) document C-62A. 40 plasma samples from patients tested for PPGLs were analyzed in three mainstream commercially available kit methods. Agreement of test results from different kit methods was assessed by Passing-Bablok regression and Bland-Altman analysis. Recalibration using uniform calibrations was performed to identify the possible calibration difference.Results: The performance of three mainstream commercially available LC-MS/MS kit methods was satisfied. The mean deviations ranges were as follows: epinephrine(E), -41.1% ~ -2.4%; norepinephrine (NE), -34.3% ~ -19.1%; metanephrine (MN), -6.5% ~ 0.3% and normetanephrine (NMN), -2.3% to 0.0%. After recalibration, the mean deviation ranges of the E and NE were reduced remarkably (E: -18% to -2.4%, NE: -19.1% to 8.0%).Conclusions: For MN and NMN, the consistency between the three commercially available kit methods is acceptable. The results for the E and NE showed unsatisfying comparability for almost all method pairs. The application of uniform calibrators could improve consistency among the mainstream systems for the measurement of plasma catecholamines.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"115-127"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0