Annals of clinical and laboratory science最新文献

{"title":"Barriers to Blood Donation: Donor Deferrals in a Saudi Hospital Setting.","authors":"Fahd A Kuriri, Abdulrahman Ahmed, Fahad Alhumud, Fehaid Alanazi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Blood donation plays a critical role in healthcare systems by supplying blood and blood products for surgeries, emergency care, and chronic disease management. In Saudi Arabia, the prevalence of genetic disorders, like sickle cell disease and thalassemia, further increase the need for a stable blood supply. However, high rates of donor deferrals remain a significant barrier to maintaining a reliable blood supply. This study aims to analyse the main factors contributing to blood donor deferrals at King Fahad Hospital Al Hofuf between January 2023 and August 2024. By identifying and correlating key deferral reasons with donor demographics, the study seeks to provide actionable insights to reduce deferral rates and enhance donor retention.</p><p><strong>Methods: </strong>This retrospective observational study analysed blood donation records of 21,149 donors, including both eligible and deferred individuals. Demographic data (age, gender, nationality) and medical assessments (hemoglobin levels, blood pressure) were collected and analysed to identify deferral trends. Descriptive and inferential statistics were applied to assess the relationships between donor characteristics and deferral reasons, with chi-square tests and logistic regression used to identify predictors of deferral.</p><p><strong>Results: </strong>Data analyses showed that 89.5% of deferred donors were male, with the largest group of deferrals aged 21-30 years. Low hemoglobin was the most common deferral reason, affecting 27.5% of all deferred donors, with female donors disproportionately affected by anemia-related deferrals. Male donors were more frequently deferred for aspirin use (13.2%) and blood pressure issues (15.9%). Seasonal trends showed deferral peaks in January and November, while the highest donation rates were recorded in April and October.</p><p><strong>Conclusion: </strong>Addressing the higher rates of deferral due to low hemoglobin and cardiovascular issues through targeted public health interventions could significantly improve donor retention and ensure a more reliable blood supply. Implementing iron supplementation programs for female donors and cardiovascular health screenings for male donors could reduce deferral rates.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"828-836"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Müllerian Hormone Enhances Migration and Invasion in Human Trophoblast Cells.","authors":"Xuhua Hu, Depeng Zhao, Wanxue Wang, Shuhua Wu, Litong Zhu, Xuemei Li, Zhuomin Huang, Jilong Yao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Anti-Müllerian hormone (AMH) belongs to the transforming growth factor-β superfamily. Recent evidence shows that AMH and its type II receptor (AMHRII) are expressed by the placenta at term. The physiological role of AMH in trophoblast invasion and migration remains to be elucidated.</p><p><strong>Methods: </strong>Immunofluorescence and western blot were used to detect the expression of AMH and AMHRII in trophoblast cell lines and chorionic-decidual mixture from early pregnancies. <i>In vitro</i> experiments, including siRNA transfection, transwell and wound healing assays were further performed to study the function of AMH in trophoblast cell line.</p><p><strong>Results: </strong>AMH and AMHR2 are mainly expressed in the interstitial trophoblast (ITB) in human chorionic-decidual mixture of early pregnancy. We found that knocking down AMH resulted in decreased migration ability of trophoblast cells compared to the control group.</p><p><strong>Conclusion: </strong>It is shown that AMH enhances trophoblast's invasion and migration abilities. AMH may play a role in embryo implantation and placenta formation.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"790-798"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histoplasmosis in a Solid Organ Transplant Patient in a Non-Endemic Region in New York City.","authors":"Sarah Shidid, Aashna Pandya, Kateryna Karpoff, Tamar A Smith-Norowitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To present the case of a solid organ transplant recipient with Histoplasmosis in New York City.</p><p><strong>Case report: </strong>We present a 39-year-old female liver transplant recipient, who experienced a two-week history of progressive shortness of breath and dyspnea on exertion that interfered with all activities of daily living. Physical examination by the team revealed the patient had a WBC of 11.7, + SARS-CoV-2 NP swab, CT Chest with pneumonia, and a urine antigen test positive for Histoplasmosis. Her overall clinical picture was concerning for new Histoplasmosis infection.</p><p><strong>Conclusion: </strong>Findings endorse the diagnosis of Histoplasmosis. Histoplasmosis is rare in non-endemic regions such as New York City. An infectious workup should include fungal markers to further evaluate shortness of breath and a need to look beyond epidemiologic measures.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"873-876"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evaluation of Urinary HE4, CEA, ProGRP, CYFRA 21-1 and NSE in the Diagnosis of Lung Cancer.","authors":"Li Zhang, Jing Zhuang, Yuxia Pang, Yinan Song, Xiaofei Li, Kuo Liu, Shuomin Li, Tao Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Urinary proteins are effective tumor biomarkers. Human epididymis protein 4 (HE4), progastrin-releasing peptide (ProGRP), carcinoembryonic antigen (CEA), cytokeratin-19 fragment 21-1(CYFRA 21-1), and neuron-specific enolase (NSE) in serum, were proposed as tumor biomarkers of lung cancer. Our aim was to identify the urine protein biomarkers that can distinguish patients with lung cancer from healthy individuals and/or patients with benign lung disease with a high level of sensitivity and specificity.</p><p><strong>Methods: </strong>These urinary protein concentrations were determined from 212 patients with lung cancer, 80 patients with benign pulmonary conditions, and 100 healthy individuals.</p><p><strong>Results: </strong>In the lung cancer group, urine HE4 median concentration was approximately ten times that in the healthy and/or pulmonary benign disease group; urine CEA, CYFRA21-1, and NSE median concentrations were about eight times, twice, and half as those in the healthy group, respectively. However, the median concentrations of urine ProGRP were almost the same in the healthy, lung benign, and cancer groups. Urine HE4 showed better specificity (94.0% vs 86.0%) and sensibility (72.38% vs 60.65%) than CEA in discriminating patients with lung cancer from healthy controls. A significantly higher ROC area was also obtained with urine HE4 than with CEA (0.87 vs 0.76).</p><p><strong>Conclusions: </strong>Urine HE4 showed the best diagnostic performance, followed by CEA. The levels of urine HE4 and CEA increased significantly in patients with lung cancer, which had little relationship with pathological type and metastasis.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"845-855"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Patient with a Delayed Diagnosis of Zinc Powder Inhalation Poisoning: A Case Report.","authors":"Min Chai, Xiuxian Zang, Xinran Yang, Chenglin Yu, Bin Nian, Yixin Chen, Dilimulat Maimaiti, Shuai Chen, Dahai Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Overexposure to zinc can lead to zinc poisoning. Zinc poisoning from zinc powder inhalation has rarely been reported.</p><p><strong>Case report: </strong>A 66-year-old male presented with nausea, chest tightness, and shortness of breath. He had no symptom improvement after antibiotic treatment. The patient's history revealed recent occupational exposure to cold zinc spray. Bronchoscopy showed a high zinc level (0.5 μmol/L) in the alveolar fluid of the patient. He was diagnosed with zinc powder inhalation poisoning (ZPIP).</p><p><strong>Results: </strong>The patient received supplemental oxygen, high-dose glucocorticoids, hemoperfusion, bronchoalveolar lavage, and supportive care. Eventually, both his symptoms and a repeat lung computed tomography scan showed improvements.</p><p><strong>Conclusion: </strong>ZPIP requires a careful history recording to avoid a missed diagnosis and delayed treatments. We present this case to share our experience regarding the clinical diagnosis of ZPIP and its treatment strategies.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"886-890"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of TRIM58 Gene Methylation-Based Minimal Residual Disease Assays in Non-Small Cell Lung Cancer for Prognosis Prediction and Treatment Decision.","authors":"Zishan Wang, Wentao Hu, Jianguang Shi, Chenwei Li, Jing Guo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To identify key genes associated with the prognosis of non-small cell lung cancer (NSCLC) through bioinformatics analysis and experimental validation, exploring the expression of the TRIM58 gene and its potential effect as a tumor suppressor.</p><p><strong>Methods: </strong>In this study, differentially expressed genes (DEGs) and differentially methylated genes (DMGs) related to lung adenocarcinoma and lung squamous cell carcinoma were selected from the TCGA dataset, with the Limma package in R software used for further filtering and intersection, followed by the assessment of the relationship between these genes and NSCLC prognosis using log-rank tests and univariate Cox regression analysis. Meanwhile, six clinical NSCLC cancer and adjacent tissue samples were collected, along with the detection of TRIM58 mRNA and protein levels using RT-PCR and Western blot.</p><p><strong>Results: </strong>The study found that low expression of TRIM58 was significantly associated with poor prognosis in NSCLC patients, while experimental data suggested that the mRNA and protein expression levels of TRIM58 in NSCLC cancer tissues were significantly lower than those in adjacent normal tissues.</p><p><strong>Conclusion: </strong>This study indicates that low expression of TRIM58 may serve as a marker for poor prognosis in NSCLC patients. The low expression of TRIM58 and its promoter methylation state may be used for detecting minimal residual disease (MRD), providing new insights into early diagnosis and prognostic assessments and aiding in formulating individualized treatment strategies. Additionally, future research should increase the sample size and intensively explore the functional mechanisms of TRIM58 so as to validate its clinical application value.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"820-827"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Exosome miR-494 on Proliferation, Migration, and Invasion of Trophoblastic Cells by Regulating the PTEN/PI3K/Akt Pathway.","authors":"Yihong Guo, Lujing Chen, Qiulin Ma, Peiyu Liu, Kun Qian","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of the exosomal miR-494 targeting phospholipinositol 3-kinase (PI3K)/protein kinase B (AKT)/rapamycin target protein (mTOR) pathway on proliferation, migration, and invasion of trophoblast cells.</p><p><strong>Methods: </strong>Decidual macrophages were randomly divided into control group, mimic NC group, miR-494 mimic group, inhibitor NC group, and miR-494 inhibitor group. Each group was transfected with corresponding miR-494 mimic NC, miR-494 mimic, and inhibitor NC and miR-494 inhibitor, while the cells of control group were only replaced with fresh medium. 48 h after transfection, the exosomes were extracted and identified by differential centrifugation. The 25 nmol/L exosomes were co-cultured with HTR-8 cells and were named as exosome control group, mimic NC exosome group, miR-494 mimic exosome group, inhibitor NC exosome group, and miR-494 inhibitor exosome group, respectively. The expression level of miR-494 in the cells was detected by qRT-PCR, cell proliferation activity was detected by CCK-8, the number of migrating cells was detected by Transwell assay, and the protein expression levels of p-PI3K, p-Akt, and p-mTOR were detected by western blot.</p><p><strong>Results: </strong>The exosomes are elliptical or crescent-shaped bilayers with particle diameters ranging from 30 nm to 150 nm, expressing CD9, CD63, and TSG101. Compared with exosome control group and mimic NC exosome group, miR-494 mimic exosome group showed increased miR-494 expression level and cell proliferation activity, increased migratory cell number, decreased PTEN protein expression, and increased P-PI3K and P-Akt expression (<i>P</i><0.05). Compared with exosome control group and inhibitor NC exosome group, miR-494 inhibitor exosome group decreased the expression level of miR-494 and cell proliferation activity, the number of migrating cells decreased, and the expression of PTEN protein increased. The protein expressions of P-PI3K, P-Akt, and P-mTOR were decreased (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>Targeted inhibition of PTEN/PI3K/Akt signaling pathway by exosome miR-494 can promote proliferation, migration, and invasion of trophoblastic cells.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"782-789"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CXCR2 Deficiency in HeLa Cell on the Regulatory Network of Coding Genes and Non-Coding RNAs.","authors":"Wei Dong, Jifang Gao, Yilin Mu, Yanyan Wang, Xiaoyan Zhu, Jiayin Wang, Chen Zi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>C-X-C motif chemokine receptor 2 (CXCR2) plays a crucial role in inflammation and immunity, and the involvement of chemokine receptors in the tumor microenvironment is extensively documented. However, the impact of CXCR2 deficiency on the complete transcriptome, including mRNA and ncRNAs, in tumor cells remains unclear.</p><p><strong>Methods: </strong>In this study, we aimed to identify differentially expressed (DE) messenger RNA (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in CXCR2 knockout HeLa cells through transcriptome sequencing and to construct regulatory networks.</p><p><strong>Results: </strong>Compared to control cells, differentially expressed mRNAs, lncRNAs and circRNAs, including 1306 coding genes, 397 lncRNAs, and 846 circRNAs were identified. Understanding these selected genes and ncRNAs could elucidate the influence of CXCR2 on tumor occurrence and progression. Furthermore, 6 DE genes, Rho guanine nucleotide exchange factor 4 (ARHGEF4), calcium voltage-gated channel auxiliary subunit alpha 2 delta 3 (CACNA2D3), fragile histidine triad (FHIT), potassium voltage-gated channel subfamily H member 5 (KCNH5), kelch like family member 1 (KLHL1) and teashirt zinc finger homeobox 2 (TSHZ2), 5 DE lncRNAs, CARMN, AL357060.1, AC098487.1, CECR7 and XLOC_009647, and 9 DE circRNAs, hsa_circ_0000196, hsa_circ_0000234, hsa_circ_0007976, hsa_circ_0008798, hsa_circ_0007766, hsa_circ_0116612, hsa_circ_0008012, hsa_circ_0004576 and hsa_circ_0118105 were verified using real-time PCR and sanger sequencing.</p><p><strong>Conclusion: </strong>Transcriptome analysis revealed that CXCR2 deficiency in HeLa cells alters the expression of coding genes and ncRNAs involved in tumor and cytokine signaling. Overall, our findings offer potential insights into the mechanisms and new research targets for cervical cancer and other tumors.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"799-809"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Liquid-Based Cytology EASYPREP and SurePath for Detection of High-Risk HPV Genotypes.","authors":"Chang-Hun Park, Chang-Woon Kim, Hyoun Wook Lee, Min-Jung Kwon","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Molecular testing for high-risk human papillomavirus (hrHPV) genotypes is important for cervical cancer screening. In this study, we compared the HPV detection rates using real-time PCR in cervical samples collected using two different liquid-based cytology (LBC) kits.</p><p><strong>Methods: </strong>Cervical swab specimens were prospectively collected using the SurePath and EASYPREP collection kits. The Roche Cobas HPV test was used to detect HPV genotypes. The agreement between HPV genotypes in samples collected with the two LBCs was evaluated based on Cohen's kappa correlation coefficient.</p><p><strong>Results: </strong>Of the 421 cervical specimens, 43(10.2%) of the SurePath-collected samples and 51(12.1%) of the EASYPREP-collected samples tested positive for hrHPV genotypes, with an absolute agreement between the two sets of samples of 97.1% (409/421, kappa=0.856, <i>P</i><0.001). When comparing hrHPV genotypes with abnormal cytology results, 60.0% (12/20) of the SurePath and 75.0% (15/20) of the EASYPREP samples tested positive for hrHPV genotypes, with an absolute agreement of 80.0% (12/15, kappa=0.667, <i>P</i>=0.002).</p><p><strong>Conclusions: </strong>Cervical samples with EASYPREP showed good agreement with SurePath samples in terms of hrHPV genotype detection. EASYPREP is worth considering as a safe alternative that can collect and preprocess samples for HPV testing.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"856-859"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Falsely Elevated HbA1c Levels in a Patient with Type 2 Diabetes Mellitus and Multiple Beta Globulin Gene Mutations.","authors":"Yuko Yamane, Midori Ishibashi, Masashi Kameyama, Toshika Okumiya, Yasuhiro Yamashiro, Masafumi Koga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We present a patient with type 2 diabetes mellitus and a variant hemoglobin whose HbA1c levels were falsely elevated regardless of the measurement method [high-performance liquid chromatography (HPLC), enzymatic, and immuno-assay] used. The causes of the falsely high HbA1c levels in this patient were investigated. The patient was a 73-year-old man with frequent hypoglycemia on self-monitoring of blood glucose, whose HbA1c level when measured by HPLC (standard mode) and immunoassay was substantially higher than that predicted by continuous blood glucose monitoring or from the patient's glycated albumin level. In addition, the HbA1c level measured by immunoassay was significantly higher than the level measured by the HPLC and enzymatic HbA1c assays. A globin gene analysis showed that this patient had Hb Hirose (β-37Trp→Ser) as well as a β-198A→G mutation in the promoter region of the gene. To clarify the causes of this apparently falsely elevated HbA1c level, measurements of <i>in vitro</i> glycation potential and erythrocyte creatine, a marker of red blood cell lifespan, were performed. Although <i>in vitro</i> glycation potential was within normal limits, the red blood cell lifespan was estimated to be 72.7 days (reference range: 55.1-66.7 days). These results suggested that an increased red blood cell lifespan contributed to the high HbA1c levels measured by the various methods used. Increased hemoglobin antigenicity due to the gene mutations in this patient may have contributed to the high HbA1c levels measured by immunoassay.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"877-885"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}