Annals of clinical and laboratory science最新文献

{"title":"Identification of Increased Tissue microRNA-214 Expression Level as a Potential Prognostic Biomarker for Gastric Cancer.","authors":"Hao Wang, Baoru Qiao, Huanbin Yang, Zhilong Si, Fei Xiao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>MicroRNAs (miRNAs) have been recognized as important candidates for diagnostic, prognostic, and predictive biomarkers for many human cancer types, including gastric cancer. Previous studies have found that the expression level of miR-214 was increased in gastric cancer tissues, and it was able to promote the proliferation, migration, and invasion of gastric cancer cells. In the present study, we aimed to investigate its clinical significance and prognostic value in gastric cancer.</p><p><strong>Methods: </strong>A total of 133 pairs of gastric cancer tissues and corresponding adjacent normal gastric tissues were obtained from 133 patients diagnosed with gastric cancer. Quantitative real-time PCR (qRT-PCR) assays were carried out to detect the expression level of miR-214. Associations between tissue miR-214 expression level and clinicopathological parameters were evaluated using Chi square test. Overall survival was calculated, and survival curves were plotted using the Kaplan-Meier method; differences between groups were compared using log-rank tests. Multivariate analysis using the Cox proportional hazards regression model was performed to assess the prognostic value of miR-214 expression.</p><p><strong>Results: </strong>We found that expression level of miR-214 was significantly higher in the gastric cancer tissues than in the adjacent normal gastric tissues (<i>P</i><0.001). The expression levels were significantly associated with depth of invasion (<i>P</i>=0.022), differentiation degree (<i>P</i>=0.009), lymph node metastasis (<i>P</i><0.001), and TNM stage (<i>P</i><0.001). The patients with high miR-214 expression level showed reduced overall five-year survival compared to those with low miR-214 expression (log-rank test <i>P</i>=0.015). Furthermore, multivariate regression analysis showed that tissue miR-214 expression level was an independent prognostic factor overall survival (HR=2.102, 95% CI: 1.572-8.822, <i>P</i>=0.033) in gastric cancer.</p><p><strong>Conclusions: </strong>Our results indicated that miR-214 was closely related to the occurrence, progression, and metastasis of gastric cancer, and overexpression of miR-214 in tissues might serve as a potential prognostic biomarker for gastric cancer.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"166-171"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143975192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annals of Clinical and Laboratory Science: Information for Authors.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"293-294"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143966979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and Effects of FUS/Sfrp5/Wnt5 in Atherosclerosis Development.","authors":"Xiaogao Wang, Hui Wang, Ran Lu, Shiyuan Chen, Yong Gao, Chaowen Yu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Atherosclerosis (AS) contributes significantly to the development of cardiovascular disease. Despite recent advances in medical treatment, the outcomes for patients with AS are still unsatisfactory. This study aims to enhance our understanding of AS and pave the way for the development of more effective therapeutic approaches to improve patient outcomes and ultimately reduce the burden of this devastating condition.</p><p><strong>Methods: </strong>An AS cell model was established using oxidized low-density lipoprotein (OX-LDL) administration to human vascular smooth muscle cells (HVSMCs), followed by injection with oe-NC, oe-FUS, oe-Sfrp5, or oe-FUS+ oe-Sfrp5. Western blot, Transwell assay, CCK8 assay, Oil Red O staining assay, and ELISA were used to elucidate the mechanisms of the FUS/Sfrp5/Wnt5 pathway in our model of AS.</p><p><strong>Results: </strong>In this study, both FUS and Sfrp5 inhibited Wnt5a expression. In addition, FUS inhibited lipid droplet formation and migration capacity of OX-LDL-induced HVSMCs, and FUS and Sfrp5 may have a synergistic effect in controlling Wnt5a in AS. These results highlight the potential therapeutic value of targeting the FUS and Sfrp5 pathway to control AS progression.</p><p><strong>Conclusion: </strong>The findings suggest that FUS and Sfrp5 may have a synergistic effect in controlling Wnt5a in AS.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"240-246"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long Noncoding RNA H19 Regulates the Foam Cell Formation of Vascular Smooth Muscle Cells by Inhibiting microRNA-107 to Activate the CD40/CD40L Pathway.","authors":"Rui Zhang, Jiani Zhou, Miaohui Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Atherosclerosis (AS) represents a life-threatening condition involving vascular inflammation and posing a high risk of death, yet effective therapeutic strategies remain limited. This research focused on elucidating the regulatory function of the long noncoding RNA H19 (lncRNA H19) in AS and its underlying molecular mechanisms.</p><p><strong>Methods: </strong>To conduct the research, an AS mouse model induced by a high-fat diet and a vascular smooth muscle cell (VSMC) model exposed to oxidized low-density lipoprotein (ox-LDL) treatment were respectively constructed.</p><p><strong>Results: </strong>There were significant aortic pathological changes and increased foam cell formation in the AS group versus the Control group. ox-LDL treatment effectively enhanced VSMC proliferation, VSMC migration, foam cell formation, and inflammatory cytokine secretion (TNF-<i>α</i> and IL-6), along with decreased microRNA-107 (miR-107) expression, while simultaneously increasing CD40 expression in VSMCs, all of which were reversed by knockdown of H19. Additionally, inhibition of miR-107 increased the migration and proliferation, inflammatory cytokine secretion, as well as foam cell formation in ox-LDL-treated VSMCs subjected to H19 knockdown. Moreover, miR-107 was confirmed to directly target CD40, and CD40 overexpression mitigated H19 knockdown-induced effects on ox-LDL-treated VSMCs.</p><p><strong>Conclusion: </strong>H19 regulates the progression of AS by modulating the CD40/CD40L axis through regulation of miR-107. Targeting H19/miR-107 and CD40/CD40L may serve as a potential treatment strategy for AS.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"247-258"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"miR-142-5p Affects Oral Squamous Cell Carcinoma by Regulating PIAS3/pSTAT3.","authors":"Jindong Dai, Hangling Wei, Guangming Yang, Bi Zhao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>miR-142-5p's role in tumors is inconsistent. The PIAS3/pSTAT3 signaling is an important regulator of the tumor microenvironment. Our study assessed whether miR-142-5p and PIAS3/pSTAT3 affect oral squamous cell carcinoma (OSCC).</p><p><strong>Methods: </strong>83 OSCC patients were recruited to analyze the baseline characteristics. In TCGA database, we found 18 miRNAs in tumor tissues, including miR-142-5p. STAT3 protein expression was measured in tissues by IHC and the relationship of miR-142-5p with PIAS3/pSTAT3 was assessed along with cellular location of miR-142-5p by immunofluorescence staining.</p><p><strong>Results: </strong>In TCGA database, 18 miRNAs were found to be expressed in OSCC samples. miR-142-5p was significantly downregulated in OSCC tissues compared to normal tissues. Meanwhile, PIAS3 has complementary sequences with hsa-miR-142-5p and luciferase activity was reduced. STAT3 was located in the nucleus of miR-142-5p-transfected cells. In OSCC, miR-142-5p activated PIAS3, leading to STAT3 activation. Patients with positive STAT3 expression had better survival outcomes than patients with negative STAT3 level.</p><p><strong>Conclusion: </strong>miR-142-5p directly targets PIAS3 and induces up-regulation of STAT3 through miR-142-5p/PIAS3/pSTAT3 axis, thereby affecting the survival outcome of OSCC patients.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"179-184"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143974675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sarcoid-like Reaction in Breast Cancer Tumor Bed and Axillary Lymph Nodes Following Neoadjuvant Chemotherapy: A Case Report.","authors":"Yan Gao, Haval Ali, Zhihong Hu, Hongxia Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Although granulomatous change is not commonly seen in breast cancer tumor bed and/or lymph node after neoadjuvant chemotherapy (NCT), they may mimic lymph node metastasis or tumor progression or recurrence. We present a case of diffuse sarcoid-like reaction (SLR) developed in both the breast tumor bed and axillary lymph nodes after NCT. A postmenopausal Hispanic woman presented with a 11.4 cm left breast mass with swollen lymph nodes in her left axilla. A core biopsy of the breast mass was performed, leading to the diagnosis of grade 3 invasive ductal carcinoma. The tumor is negative for estrogen receptor, progesterone receptor, and human epidermal growth factor receoptor-2 overexpression. The patient received NCT with three cycles of doxorubicin and cyclophosphamide, followed by weekly paclitaxel for 12 weeks. After NCT, the tumor in her left breast significantly reduced in size, but the lymph nodes remained swollen. She subsequently underwent left modified radical mastectomy. Histological examination of the treated tumor bed revealed residual invasive tumor with frequent non-caseating granulomatous change. The granulomatous reaction was also seen in several axillary lymph nodes, of which only one had residual metastatic tumor cells. Idiopathic granulomatous mastitis, sarcoidosis, and infective etiologies were excluded based on the patient's medical history, imaging, and histological findings. We report a case of localized SLR in response to NCT in breast cancer tumor bed and axillary lymph nodes. Recognizing this feature is important to avoid misdiagnosis and overtreatment of SLR as residual cancer.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"281-285"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple Lines of Treatment in a Patient with Small Cell Neuroendocrine Carcinoma of the Cervix: A Case Report.","authors":"Liyan Cao","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Small cell neuroendocrine carcinoma of the cervix (SCNEC) is a rare and highly aggressive tumor with a high rate of recurrence and metastasis. Due to its low prevalence, standardized treatment regimens for SCNEC remain undefined. This case report describes an adult patient with SCNEC who developed multiple metastases, including those in the neck, abdominal wall, and brain. The patient underwent a multidisciplinary treatment approach, including chemoradiotherapy, targeted therapy, and a PD-1 inhibitor combined with radiotherapy, achieving notable therapeutic efficacy with an overall survival exceeding 49 months. This case underscores the potential role of immunotherapy in treating SCNEC. Furthermore, the extensive tumor progression, exacerbated by disruptions during the COVID-19 pandemic and poor patient compliance highlights the importance of cervical cancer screening, early diagnosis, and treatment compliance.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"286-292"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143962834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Information About The Association of Clinical Scientists.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"295"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autologous Platelet-Rich Plasma Combined with Endometrial Microstimulation to Improve Thin Endometrium Status and Clinical Outcomes: A Prospective Controlled Study.","authors":"Huakun Zhang, Xuhua Hu, Wanxue Wang, Xuanying Deng, Xuemei Li, Zhihong Fu, Chunrong Qin, Jilong Yao, Zhengzhong Wu, Shuhua Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effect of a novel intrauterine infusion of autologous platelet-rich plasma (PRP) on endometrial condition and clinical outcomes in patients with thin endometrium undergoing frozen-thawed embryo transfer (FET) cycles.</p><p><strong>Methods: </strong>A prospective randomized controlled trial was conducted with 70 patients diagnosed with thin endometrium at the Reproductive Medicine Center of Shenzhen Maternal and Child Health Hospital from January 2022 to June 2023. The patients were randomly divided into two groups: a single PRP infusion group and a PRP combined with endometrial microstimulation (EM+PRP) group. Endometrial thickness and classification were assessed before and after treatment. Clinical outcomes, including human chorionic gonadotropin (HCG) positivity rate, embryo implantation rate, clinical pregnancy rate, and early miscarriage rate, were analyzed.</p><p><strong>Results: </strong>Baseline characteristics, including pre-treatment endometrial thickness, were comparable between groups. Both groups showed a significant increase in endometrial thickness after treatment (<i>P</i><0.05). The EM+PRP group exhibited a greater improvement in post-treatment endometrial thickness compared to the single PRP group (<i>P</i><0.05). Endometrial morphology showed improvement in both groups, but the difference was not statistically significant (<i>P</i>>0.05). No significant differences were found between the two groups in terms of HCG positivity rate, embryo implantation rate, clinical pregnancy rate, or early miscarriage rate.</p><p><strong>Conclusion: </strong>Both EM+PRP and single PRP intrauterine infusion effectively increased endometrial thickness and improved clinical outcomes in patients with thin endometrium. The combined EM+PRP therapy showed potential for greater efficacy in improving endometrial condition, warranting further research in larger, multicenter studies.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"185-191"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Hidden Threat in Immunoassays: A Case of Persistent False-Positive CK-MB.","authors":"Siwei Xue, Weiqiang Huang, Degui Ou, Wenda Huang, Jianwen Liu, Zetian Zhong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This report aims to explore the significant impact of heterophilic antibodies on CK-MB immunoassays, leading to persistent false-positive results.</p><p><strong>Case report: </strong>A 59-year-old male with a history of coronary artery disease exhibited persistently elevated CK-MB levels for over two years, despite normal troponin I levels. Further investigations, including serial dilution, blocking experiments, and testing across different platforms, confirmed the presence of heterophilic antibody interference, which was responsible for the falsely elevated CK-MB levels.</p><p><strong>Conclusion: </strong>This case underscores the critical importance of recognizing heterophilic antibody interference in immunoassays. The use of appropriate diagnostic approaches, such as blocking antibodies, cross-platform testing, and the integration of additional diagnostic markers, is essential to prevent misdiagnosis and ensure accurate clinical decision-making.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 2","pages":"272-276"},"PeriodicalIF":1.1,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143964196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}