Annals of clinical and laboratory science最新文献

{"title":"Information About The Association of Clinical Scientists.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"384"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of <i>Chlamydia trachomatis</i> Pgp3 Antibody in Human Sera.","authors":"Tamar A Smith-Norowitz, Sara Abdelrahaman, Sabah N Islam, Haram Abdelmajid, Rauno Joks, Margaret R Hammerschlag, Stephan A Kohlhoff","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong><i>Chlamydia trachomatis</i> is the most common bacterial sexually transmitted infection (STI) worldwide. Genital infection with <i>C. trachomatis</i> in women can cause ascending infection leading to pelvic inflammatory disease. Studies have suggested that the Pgp3 protein encoded by <i>C. trachomatis</i> plasmid may contribute to long term inflammatory complications of <i>C. trachomatis</i> infection (tubal factor infertility). This study assessed <i>C. trachomatis</i>-specific Pgp3 IgG antibody levels in sera from adolescents and young adults in an inner-city population in New York.</p><p><strong>Methods: </strong>Blood was obtained from patients seen in a primary care setting as part of a <i>C. trachomatis</i> seroepidemiology study. Serum samples were selected based on <i>C. trachomatis</i> IgG status (anti-MOMP, Labsystems). <i>C. trachomatis</i> anti-Pgp3 antibodies were assayed in sera from both <i>C. trachomatis</i> IgG positive and negative subjects.</p><p><strong>Results: </strong>We identified 39 participants, 16-20 y/o (mean age 18±1.2). The number of <i>C. trachomatis</i> IgG positive or negative subjects was 24 and 15, respectively; of the IgG positive subjects, 15% were males, and 85% were females. The mean distribution (OD value) of Pgp3 antibody responses in IgG positive versus negative subjects was 1.03±0.32 and 1.08±0.31 respectively; <i>P</i>=0.7833. The mean difference for Pgp3 responses was 0.0507±0.31, <i>P</i>=0.6266 (two sample <i>t</i>-test).</p><p><strong>Conclusion: </strong><i>C. trachomatis</i> Pgp3 antibody responses were similar in <i>C. trachomatis</i> anti-MOMP IgG positive and negative subjects.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"265-269"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Performance Evaluation of the AccuPower Hepatitis C Virus Quant Assay Compared to the Cobas 6800 Hepatitis C Virus Assay.","authors":"Byoung-Sun Mun, Qute Choi, Hyun-Jin Kim, Jung Yoon, Soo-Young Yoon","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Hepatitis C virus (HCV) causes chronic liver disease, affecting 58 million people globally. Although antibody testing is cost-effective for screening, nucleic acid testing (NAT) enables early detection and treatment monitoring. Automated systems improve HCV RNA quantification efficiency. This study evaluates the lyophilized AccuPower HCV Quant Kit on ExiStation FA, comparing its performance with Cobas 6800 for viral load testing.</p><p><strong>Methods: </strong>AccuPower kit was evaluated on ExiStation FA96. Precision, linearity, limit of detection (LOD), and lower limit of quantitation (LLOQ) were assessed using World Health Organization (WHO) standards. Agreement and correlation were evaluated by comparing results with the Cobas HCV test using 368 clinical serum samples.</p><p><strong>Results: </strong>The assay demonstrated intra- and inter-assay variability lower than CVs of 5% across evaluated concentrations and showed strong linearity with expected concentration (R²=0.99). The LOD and LLOQ of the assay were 3.37 IU/mL and 7.50 IU/mL, respectively. Clinical comparison showed almost perfect agreement (κ=0.98) and correlation (r=0.971). Bland-Altman analysis revealed minimal bias (mean bias of -0.27±0.67 log₁₀ IU/mL).</p><p><strong>Conclusions: </strong>The AccuPower HCV Quant Kit used with the ExiStation FA system provides reliable and accurate HCV RNA quantification with operational advantages, supporting its suitability for both HCV screening and clinical management.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"279-289"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annals of Clinical and Laboratory Science: Information for Authors.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"382-383"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of the International System for Reporting Serous Fluid Cytology (ISRSFC) and AgNOR Staining for Improved Diagnostic Accuracy in Serous Effusions.","authors":"Magdi M Salih, Shatha A Alotaibi, Adil A M Yousif, Tariq E Elmissbah, Eman H Khalifa","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Serous effusion smears categorized as suspicious for malignancy (SFM) pose significant diagnostic challenges. This study aimed to evaluate the diagnostic utility of argyrophilic nucleolar organizer region (AgNOR) quantification across International System for Reporting Serous Fluid Cytology (ISRS-FC) categories and to assess its association with increasing cytological atypia and malignancy risk.</p><p><strong>Methods: </strong>A total of 160 serous effusion samples collected between October 2024 and June 2025 were examined using Papanicolaou and AgNOR staining. Cases were classified into negative for malignancy (NFM), atypia of undetermined significance (AUS), suspicious for malignancy (SFM), and malignant (MAL). Mean Ag-NOR counts were compared across categories using appropriate statistical analyses.</p><p><strong>Results: </strong>Mean Ag-NOR counts (mean±SD) increased progressively from NFM (5.64±4.45) and AUS (5.98±5.15) to SFM (7.66±6.62) and MAL (8.64±7.81). One-way ANOVA demonstrated a significant difference among groups (<i>p</i>=0.001). Post hoc analysis confirmed significantly higher counts in SFM and MAL compared with NFM, and in MAL compared with AUS (<i>p</i><0.05).</p><p><strong>Conclusion: </strong>AgNOR quantification provides objective proliferative information that may enhance diagnostic stratification in serous effusion cytology, particularly in indeterminate categories. Further multicenter studies are required to establish standardized cut-off values before routine clinical application.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"303-309"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphous Low-Grade Neuroepithelial Tumor of the Young (PLNTY): A Rare Entity.","authors":"David M Berger","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This report describes a case of polymorphous low-grade neuroepithelial tumor of the young (PLNTY) with an unusual immunohistochemical profile that was diagnosed with next-generation sequencing.</p><p><strong>Case report: </strong>An 11-year-old female presented with a seizure and was found to have a 3.3 cm right temporal calcified mass with mild heterogeneous enhancement most consistent with a low-grade glioma. The mass was biopsied. Sections showed a moderately cellular neoplasm composed of oval to elongate cells with clumped chromatin, scant cytoplasm, irregular nuclear contours, and rare mitotic figures within a fibrillary background with delicate branching capillaries and microcalcifications. The neoplastic cells were diffusely positive for synaptophysin and negative for NeuN. Additionally, the cells had a patchy positivity for CD34 and p16, rare positivity for SOX10 and GFAP, strong positivity for p53 in 20% of cells, absence of <i>BRAF</i> V600E and <i>IDH1</i> R132H mutant proteins, retained nuclear expression of ATRX, and a Ki67 index of 3%. Next-generation sequencing revealed an <i>FGFR2::SHTN1</i> fusion.</p><p><strong>Conclusion: </strong>This case illustrates the increasing centrality of molecular testing in the diagnosis of central nervous system (CNS) tumors and the impact of recent advances in CNS tumor classification on the care of pediatric patients.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"339-340"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576908","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myeloid Neoplasm with Eosinophilia Harboring a Novel <i>CCDC6::PDGFRB</i> fusion: Diagnostic Challenge and Therapeutic Implications.","authors":"Vasily Ovechko, Anindita Ghosh, Sofia Garces, Guilin Tang, C Cameron Yin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Myeloid/lymphoid neoplasms with eosinophilia and tyrosine kinase gene fusions are rare hematologic malignancies characterized by constitutive kinase activation and sensitivity to tyrosine kinase inhibitors. <i>PDGFRB</i> rearrangements are uncommon, and <i>CCDC6::PDGFRB</i> fusion has been reported only rarely.</p><p><strong>Case report: </strong>A 44-year-old man presented with leukocytosis, eosinophilia, anemia, splenomegaly, and constitutional symptoms. A bone marrow examination showed granulocytic hyperplasia with marked eosinophilia. Cytogenetic analysis revealed t(5;10)(q32;q21), while optical genome mapping identified a <i>CCDC6::PDGFRB</i> fusion, confirmed by fluorescence in situ hybridization. No additional pathogenic mutations were detected by targeted next-generation sequencing. Treatment with low-dose imatinib achieved rapid hematologic remission, which was maintained at 22 months.</p><p><strong>Conclusions: </strong>This case highlights the importance of comprehensive genomic testing in patients with eosinophilia-associated myeloid neoplasms for identifying rare and therapeutically actionable <i>PDGFRB</i> rearrangements.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"350-353"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"KRAS-Driven Combined Pulmonary Adenocarcinoma and Atypical Carcinoid Tumor: A Rare Case with Shared Clonal Origins.","authors":"Alexander B Banerjee, Jaiyeola Thomas-Ogunniyi, Navneet Narula, Mahmoud Elsayad","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We report a rare case of a combined pulmonary tumor composed of adenocarcinoma and atypical carcinoid, both sharing identical KRAS p.G12S mutations and high PD-L1 expression. A 74-year-old woman with a remote history of stage IV colon cancer presented with acute bilateral pulmonary embolism and was subsequently found to have a right upper lobe (RUL) lung nodule. Histologic examination of the biopsy and subsequent resection material revealed two morphologically distinct tumor components: adenocarcinoma and an atypical carcinoid. Immunohistochemical stains showed divergent marker profiles, while next-generation sequencing (NGS) and PD-L1 immunostaining demonstrated shared KRAS mutation and similar expression patterns, indicating a clonal relationship. To our knowledge, this represents the first reported case of a KRAS-mutated adenocarcinoma and atypical carcinoid tumor with molecular confirmation of clonality. This case underscores the critical role of integrated histologic and molecular analysis in the diagnosis and classification of combined lung tumors.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"365-369"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Single-Capillary Dual Point-of-Care Testing to Support Cardio-Renal Diagnostic Decision-Making in Ambulatory Care.","authors":"Perrin Ngougni Pokem, Ali Khatib, Florian Belik, Álvaro Martínez Ortega, Iman Azariouh Kaddouri, Soukayna Draoui, Laura Labriola, Arnaud Devresse, Anne-Catherine Pouleur, Damien Gruson","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Integrated interpretation of renal function and natriuretic peptides is essential in ambulatory cardio-renal care. This study evaluated whether a single-capillary dual point-of-care testing (POCT) strategy combining creatinine/eGFR and NT-proBNP could support real-time, integrated clinical decision-making during a single consultation.</p><p><strong>Methods: </strong>In this exploratory, single-center, real-world study that was designed as a feasibility study focusing on workflow integration and clinical usability, capillary creatinine/eGFR and NT-proBNP were evaluated using a single-fingerstick dual-POCT workflow in two exploratory clinical subgroups recruited according to their respective clinical indications. Analytical agreement was assessed using Passing-Bablok regression (slope, intercept, and 95% confidence intervals) and Bland-Altman analysis. Agreement at guideline-based clinical thresholds (eGFR ≤60 mL/min/1.73m²; NT-proBNP ≥125 ng/L) was evaluated using Cohen's kappa. Workflow feasibility and time-to-result were also documented.</p><p><strong>Results: </strong>Forty-three patients underwent creatinine/eGFR testing, and a separate subgroup of 22 patients underwent NT-proBNP testing, reflecting different clinical indications and real-world recruitment during this exploratory feasibility study. Passing-Bablok regression showed good agreement with slopes close to unity and no significant systematic bias. Bland-Altman analysis demonstrated clinically acceptable limits of agreement. Diagnostic agreement at clinical thresholds was substantial for eGFR (κ=0.71) and perfect for NT-proBNP (κ=1.00), with no clinically relevant misclassification. Dual biomarker results were available within approximately 15 minutes, enabling same-visit clinical decisions.</p><p><strong>Conclusions: </strong>A single-capillary dual-biomarker POCT workflow enables rapid and reliable cardio-renal assessment within one ambulatory visit. Its primary value lies in integrated, threshold-based clinical interpretation enabled by the simultaneous availability of complementary biomarkers, rather than analytical comparison alone.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"290-294"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intervention Study of Virtual Reality Technology on Sympathetic Nerves and Emotion in Patients with Acute Coronary Syndrome.","authors":"Yaping Zhou, Dongming Lin, Min Zhu, Shuwei Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the effects of virtual reality (VR) technology on the sympathetic nervous system and the emotional status in patients with acute coronary syndrome (ACS).</p><p><strong>Methods: </strong>A total of 102 patients with ACS, who were treated in the department of cardiology at the Second Affiliated Hospital of Zhejiang Chinese Medical University from December 2022 to December 2024, were enrolled into the study. Then, they were randomly divided into a control group (n=50) and an intervention group (n=52) using a random number table. The control group received conventional treatment, while the intervention group underwent virtual reality (VR) therapy combined with conventional treatment. The differences in heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP), time-domain indices of heart rate variability (HRV), standard deviation of the normal respiratory rate (RR) intervals (SDNN), standard deviation of the average normal-to-normal (NN) [NS1.1] intervals in 5-minute segments of an entire recording (SDANN), root-mean-square of the successive differences of adjacent RR intervals (RMSSD), low frequency(LF)/high frequency (HF) ratio, LF, HF, three catecholamine indicators [norepinephrine (NE), epinephrine (E), dopamine (DA)], and psychological status assessed by Self-rating Anxiety Scale (SAS) and Hospital Anxiety and Depression Scale (HADS) were observed and calculated before and after treatment in both groups.</p><p><strong>Results: </strong>The increases in ∆SDNN and ∆SDANN in the intervention group were significantly greater than those in the control group, and the decreases in ∆LF, ∆LF/ HF ratio, ∆NE, ∆SAS and ∆HADS in the intervention group were significantly greater than those in the control group (all <i>P</i><0.05).</p><p><strong>Conclusion: </strong>VR technology can alleviate the hyperactivity of the sympathetic nervous system and improve anxiety in patients with ACS.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 3","pages":"319-323"},"PeriodicalIF":0.9,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148576833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}