Identification of Increased Tissue microRNA-214 Expression Level as a Potential Prognostic Biomarker for Gastric Cancer.

IF 1.1 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Hao Wang, Baoru Qiao, Huanbin Yang, Zhilong Si, Fei Xiao
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引用次数: 0

Abstract

Objective: MicroRNAs (miRNAs) have been recognized as important candidates for diagnostic, prognostic, and predictive biomarkers for many human cancer types, including gastric cancer. Previous studies have found that the expression level of miR-214 was increased in gastric cancer tissues, and it was able to promote the proliferation, migration, and invasion of gastric cancer cells. In the present study, we aimed to investigate its clinical significance and prognostic value in gastric cancer.

Methods: A total of 133 pairs of gastric cancer tissues and corresponding adjacent normal gastric tissues were obtained from 133 patients diagnosed with gastric cancer. Quantitative real-time PCR (qRT-PCR) assays were carried out to detect the expression level of miR-214. Associations between tissue miR-214 expression level and clinicopathological parameters were evaluated using Chi square test. Overall survival was calculated, and survival curves were plotted using the Kaplan-Meier method; differences between groups were compared using log-rank tests. Multivariate analysis using the Cox proportional hazards regression model was performed to assess the prognostic value of miR-214 expression.

Results: We found that expression level of miR-214 was significantly higher in the gastric cancer tissues than in the adjacent normal gastric tissues (P<0.001). The expression levels were significantly associated with depth of invasion (P=0.022), differentiation degree (P=0.009), lymph node metastasis (P<0.001), and TNM stage (P<0.001). The patients with high miR-214 expression level showed reduced overall five-year survival compared to those with low miR-214 expression (log-rank test P=0.015). Furthermore, multivariate regression analysis showed that tissue miR-214 expression level was an independent prognostic factor overall survival (HR=2.102, 95% CI: 1.572-8.822, P=0.033) in gastric cancer.

Conclusions: Our results indicated that miR-214 was closely related to the occurrence, progression, and metastasis of gastric cancer, and overexpression of miR-214 in tissues might serve as a potential prognostic biomarker for gastric cancer.

组织microRNA-214表达水平升高作为胃癌潜在预后生物标志物的鉴定
目的:MicroRNAs (miRNAs)已被认为是许多人类癌症类型(包括胃癌)的诊断、预后和预测生物标志物的重要候选物。既往研究发现,miR-214在胃癌组织中表达水平升高,能够促进胃癌细胞的增殖、迁移和侵袭。在本研究中,我们旨在探讨其在胃癌中的临床意义和预后价值。方法:133例确诊胃癌患者的胃癌组织及相应的癌旁正常胃组织共133对。采用实时荧光定量PCR (qRT-PCR)检测miR-214的表达水平。采用卡方检验评估组织miR-214表达水平与临床病理参数的相关性。计算总生存期,采用Kaplan-Meier法绘制生存曲线;采用log-rank检验比较组间差异。采用Cox比例风险回归模型进行多因素分析,评估miR-214表达的预后价值。结果:我们发现miR-214在胃癌组织中的表达水平明显高于邻近正常胃组织(PP=0.022)、分化程度(P=0.009)、淋巴结转移(PPP=0.015)。多因素回归分析显示,组织miR-214表达水平是胃癌患者预后的独立因素(HR=2.102, 95% CI: 1.572 ~ 8.822, P=0.033)。结论:我们的研究结果表明,miR-214与胃癌的发生、进展和转移密切相关,组织中miR-214的过表达可能是胃癌的潜在预后生物标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Annals of clinical and laboratory science
Annals of clinical and laboratory science 医学-医学实验技术
CiteScore
1.60
自引率
0.00%
发文量
112
审稿时长
6-12 weeks
期刊介绍: The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.
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