Annals of clinical and laboratory science最新文献

{"title":"Application of TRIM58 Gene Methylation-Based Minimal Residual Disease Assays in Non-Small Cell Lung Cancer for Prognosis Prediction and Treatment Decision.","authors":"Zishan Wang, Wentao Hu, Jianguang Shi, Chenwei Li, Jing Guo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To identify key genes associated with the prognosis of non-small cell lung cancer (NSCLC) through bioinformatics analysis and experimental validation, exploring the expression of the TRIM58 gene and its potential effect as a tumor suppressor.</p><p><strong>Methods: </strong>In this study, differentially expressed genes (DEGs) and differentially methylated genes (DMGs) related to lung adenocarcinoma and lung squamous cell carcinoma were selected from the TCGA dataset, with the Limma package in R software used for further filtering and intersection, followed by the assessment of the relationship between these genes and NSCLC prognosis using log-rank tests and univariate Cox regression analysis. Meanwhile, six clinical NSCLC cancer and adjacent tissue samples were collected, along with the detection of TRIM58 mRNA and protein levels using RT-PCR and Western blot.</p><p><strong>Results: </strong>The study found that low expression of TRIM58 was significantly associated with poor prognosis in NSCLC patients, while experimental data suggested that the mRNA and protein expression levels of TRIM58 in NSCLC cancer tissues were significantly lower than those in adjacent normal tissues.</p><p><strong>Conclusion: </strong>This study indicates that low expression of TRIM58 may serve as a marker for poor prognosis in NSCLC patients. The low expression of TRIM58 and its promoter methylation state may be used for detecting minimal residual disease (MRD), providing new insights into early diagnosis and prognostic assessments and aiding in formulating individualized treatment strategies. Additionally, future research should increase the sample size and intensively explore the functional mechanisms of TRIM58 so as to validate its clinical application value.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"820-827"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>In Memoriam</i>: Frederick L. Kiechle, MD, PhD (1946-2024).","authors":"Charles D Hawker, Myra L Wilkerson","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"719-720"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of CXCR2 Deficiency in HeLa Cell on the Regulatory Network of Coding Genes and Non-Coding RNAs.","authors":"Wei Dong, Jifang Gao, Yilin Mu, Yanyan Wang, Xiaoyan Zhu, Jiayin Wang, Chen Zi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>C-X-C motif chemokine receptor 2 (CXCR2) plays a crucial role in inflammation and immunity, and the involvement of chemokine receptors in the tumor microenvironment is extensively documented. However, the impact of CXCR2 deficiency on the complete transcriptome, including mRNA and ncRNAs, in tumor cells remains unclear.</p><p><strong>Methods: </strong>In this study, we aimed to identify differentially expressed (DE) messenger RNA (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in CXCR2 knockout HeLa cells through transcriptome sequencing and to construct regulatory networks.</p><p><strong>Results: </strong>Compared to control cells, differentially expressed mRNAs, lncRNAs and circRNAs, including 1306 coding genes, 397 lncRNAs, and 846 circRNAs were identified. Understanding these selected genes and ncRNAs could elucidate the influence of CXCR2 on tumor occurrence and progression. Furthermore, 6 DE genes, Rho guanine nucleotide exchange factor 4 (ARHGEF4), calcium voltage-gated channel auxiliary subunit alpha 2 delta 3 (CACNA2D3), fragile histidine triad (FHIT), potassium voltage-gated channel subfamily H member 5 (KCNH5), kelch like family member 1 (KLHL1) and teashirt zinc finger homeobox 2 (TSHZ2), 5 DE lncRNAs, CARMN, AL357060.1, AC098487.1, CECR7 and XLOC_009647, and 9 DE circRNAs, hsa_circ_0000196, hsa_circ_0000234, hsa_circ_0007976, hsa_circ_0008798, hsa_circ_0007766, hsa_circ_0116612, hsa_circ_0008012, hsa_circ_0004576 and hsa_circ_0118105 were verified using real-time PCR and sanger sequencing.</p><p><strong>Conclusion: </strong>Transcriptome analysis revealed that CXCR2 deficiency in HeLa cells alters the expression of coding genes and ncRNAs involved in tumor and cytokine signaling. Overall, our findings offer potential insights into the mechanisms and new research targets for cervical cancer and other tumors.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"799-809"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Exosome miR-494 on Proliferation, Migration, and Invasion of Trophoblastic Cells by Regulating the PTEN/PI3K/Akt Pathway.","authors":"Yihong Guo, Lujing Chen, Qiulin Ma, Peiyu Liu, Kun Qian","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of the exosomal miR-494 targeting phospholipinositol 3-kinase (PI3K)/protein kinase B (AKT)/rapamycin target protein (mTOR) pathway on proliferation, migration, and invasion of trophoblast cells.</p><p><strong>Methods: </strong>Decidual macrophages were randomly divided into control group, mimic NC group, miR-494 mimic group, inhibitor NC group, and miR-494 inhibitor group. Each group was transfected with corresponding miR-494 mimic NC, miR-494 mimic, and inhibitor NC and miR-494 inhibitor, while the cells of control group were only replaced with fresh medium. 48 h after transfection, the exosomes were extracted and identified by differential centrifugation. The 25 nmol/L exosomes were co-cultured with HTR-8 cells and were named as exosome control group, mimic NC exosome group, miR-494 mimic exosome group, inhibitor NC exosome group, and miR-494 inhibitor exosome group, respectively. The expression level of miR-494 in the cells was detected by qRT-PCR, cell proliferation activity was detected by CCK-8, the number of migrating cells was detected by Transwell assay, and the protein expression levels of p-PI3K, p-Akt, and p-mTOR were detected by western blot.</p><p><strong>Results: </strong>The exosomes are elliptical or crescent-shaped bilayers with particle diameters ranging from 30 nm to 150 nm, expressing CD9, CD63, and TSG101. Compared with exosome control group and mimic NC exosome group, miR-494 mimic exosome group showed increased miR-494 expression level and cell proliferation activity, increased migratory cell number, decreased PTEN protein expression, and increased P-PI3K and P-Akt expression (<i>P</i><0.05). Compared with exosome control group and inhibitor NC exosome group, miR-494 inhibitor exosome group decreased the expression level of miR-494 and cell proliferation activity, the number of migrating cells decreased, and the expression of PTEN protein increased. The protein expressions of P-PI3K, P-Akt, and P-mTOR were decreased (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>Targeted inhibition of PTEN/PI3K/Akt signaling pathway by exosome miR-494 can promote proliferation, migration, and invasion of trophoblastic cells.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"782-789"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Challenging Case of Myeloid Sarcoma Misdiagnosed as High-Grade B-Cell Lymphoma.","authors":"Ahmed A Ahmed, Nourhan Ibrahim, Amer Wahed, Brenda Mai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>A 46-year-old female presented with back pain associated with progressive bilateral lower extremity weakness and paresthesia. Imaging studies revealed a retroperitoneal mass with severe spinal compression. Histological sections showed blastoid cells with large nuclei, irregular membranes, fine chromatin, and prominent nucleoli. Immunohistochemical stains (IHC) showed neoplastic cells positive for B-cell markers. This patient was diagnosed with a high-grade B-cell lymphoma before transferred to our institution for further work-up. After review of the case, additional IHC was requested which revealed positivity for CD117 and myeloperoxidase (MPO). The overall morphological and immunophenotypical features were most compatible with myeloid sarcoma with aberrant expression of B-cell markers and this patient's diagnosis was amended. A literature review showed that 40-50% of myeloid sarcomas are misdiagnosed as lymphoma since they can frequently stain with B-cell or T-cell markers, which makes it challenging for an accurate diagnosis and sub-classification. We present this case to raise awareness of the potential diagnostic pitfalls.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"867-872"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Liquid-Based Cytology EASYPREP and SurePath for Detection of High-Risk HPV Genotypes.","authors":"Chang-Hun Park, Chang-Woon Kim, Hyoun Wook Lee, Min-Jung Kwon","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Molecular testing for high-risk human papillomavirus (hrHPV) genotypes is important for cervical cancer screening. In this study, we compared the HPV detection rates using real-time PCR in cervical samples collected using two different liquid-based cytology (LBC) kits.</p><p><strong>Methods: </strong>Cervical swab specimens were prospectively collected using the SurePath and EASYPREP collection kits. The Roche Cobas HPV test was used to detect HPV genotypes. The agreement between HPV genotypes in samples collected with the two LBCs was evaluated based on Cohen's kappa correlation coefficient.</p><p><strong>Results: </strong>Of the 421 cervical specimens, 43(10.2%) of the SurePath-collected samples and 51(12.1%) of the EASYPREP-collected samples tested positive for hrHPV genotypes, with an absolute agreement between the two sets of samples of 97.1% (409/421, kappa=0.856, <i>P</i><0.001). When comparing hrHPV genotypes with abnormal cytology results, 60.0% (12/20) of the SurePath and 75.0% (15/20) of the EASYPREP samples tested positive for hrHPV genotypes, with an absolute agreement of 80.0% (12/15, kappa=0.667, <i>P</i>=0.002).</p><p><strong>Conclusions: </strong>Cervical samples with EASYPREP showed good agreement with SurePath samples in terms of hrHPV genotype detection. EASYPREP is worth considering as a safe alternative that can collect and preprocess samples for HPV testing.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"856-859"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Falsely Elevated HbA1c Levels in a Patient with Type 2 Diabetes Mellitus and Multiple Beta Globulin Gene Mutations.","authors":"Yuko Yamane, Midori Ishibashi, Masashi Kameyama, Toshika Okumiya, Yasuhiro Yamashiro, Masafumi Koga","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>We present a patient with type 2 diabetes mellitus and a variant hemoglobin whose HbA1c levels were falsely elevated regardless of the measurement method [high-performance liquid chromatography (HPLC), enzymatic, and immuno-assay] used. The causes of the falsely high HbA1c levels in this patient were investigated. The patient was a 73-year-old man with frequent hypoglycemia on self-monitoring of blood glucose, whose HbA1c level when measured by HPLC (standard mode) and immunoassay was substantially higher than that predicted by continuous blood glucose monitoring or from the patient's glycated albumin level. In addition, the HbA1c level measured by immunoassay was significantly higher than the level measured by the HPLC and enzymatic HbA1c assays. A globin gene analysis showed that this patient had Hb Hirose (β-37Trp→Ser) as well as a β-198A→G mutation in the promoter region of the gene. To clarify the causes of this apparently falsely elevated HbA1c level, measurements of <i>in vitro</i> glycation potential and erythrocyte creatine, a marker of red blood cell lifespan, were performed. Although <i>in vitro</i> glycation potential was within normal limits, the red blood cell lifespan was estimated to be 72.7 days (reference range: 55.1-66.7 days). These results suggested that an increased red blood cell lifespan contributed to the high HbA1c levels measured by the various methods used. Increased hemoglobin antigenicity due to the gene mutations in this patient may have contributed to the high HbA1c levels measured by immunoassay.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"877-885"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Critical Perspectives on Global Health Policy, Diagnostic Access and Practice Gaps towards a Tuberculosis Free India.","authors":"Biswajit Chakraborty, Amaj A Laskar, Anamika Baruah, Rinki Kumar, Mustafa A Barbhuiya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Tuberculosis (TB) poses a significant public health challenge in India. According to the World Health Organization (WHO), India tops the list of 30 countries responsible for 87% of global TB cases in 2023. Recent data indicates that India's TB incidence rate was 195 cases per 100,000 population in 2023, accounting for approximately 26% of global TB cases. Global health organizations like the WHO and the Centers for Disease Control and Prevention (CDC) emphasize molecular techniques, such as Cartridge-Based Nucleic Acid Amplification Test (CB-NAAT), sequencing, and serological tests, to improve the accuracy and efficiency of TB diagnosis. The Government of India initiated the National Tuberculosis Elimination Program (NTEP), aiming for a TB-free India by 2025. Despite the efforts, practice gaps remain in diagnosis, treatment, and monitoring. Accelerated strategies focusing on early detection, management of latent TB, and control of drug resistance are critical. This review explores global guidelines, highlights implementation challenges, and suggests pathways for achieving effective TB control in India.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"721-738"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ACSL4 Regulates LPS-Induced Ferroptosis in Cardiomyocytes through FASN.","authors":"Renxian Gao, Fanshun Ou, Jianfeng Lin, Jihao Chen, Zhang Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Myocardial injury is a prevalent complication of sepsis. This study aims to shed light on the role of Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4) in regulating Fatty Acid Synthase (FASN) to identify the intrinsic molecular mechanisms of sepsis-induced myocardial injury.</p><p><strong>Method: </strong>H9c2 cells were treated with Lipopolysaccharide (LPS) to model sepsis-induced cardiomyocyte injury and were subsequently divided into seven groups: Control, LPS, LPS+sh-NC, LPS+sh-ACSL4, LPS+sh-ACSL4+Erastin, LPS+sh-ACSL4+oe-NC, and LPS+sh-ACSL4+oe-FASN. Immunofluorescence staining and Western blot analysis were used to assess the expression levels of ACSL4, FASN, GPX4, and FTH1. Co-immunoprecipitation was conducted to investigate the interaction between ACSL4 and FASN. Additionally, levels of LDH, MDA, GSH, SOD, and iron were measured. We employed the CCK-8 assay and flow cytometry to determine cell viability and apoptosis rates.</p><p><strong>Result: </strong>Compared with the control group, the LPS group showed decreased cell viability, increased apoptosis rate, elevated levels of LDH, MDA, and iron, reduced GSH and SOD levels, upregulated ACSL4 and FASN expression, and downregulated GPX4 and FTH1 expression. Treatment with sh-ACSL4 helped to ameliorate these changes. In the LPS+sh-ACSL4+Erastin group, cell viability declined further, apoptosis rate increased, and LDH, MDA, and iron levels were elevated, while GSH and SOD levels decreased, and GPX4 and FTH1 expression were reduced compared with the LPS+sh-ACSL4 group. Co-immunoprecipitation revealed an interaction between ACSL4 and FASN. Knockdown of ACSL4 combined with FASN overexpression attenuated the protective influence of ACSL4 knockdown on H9c2 cells.</p><p><strong>Conclusion: </strong>ACSL4 may play an important role in LPS-induced cardiomyocyte injury by influencing the process of ferroptosis via FASN.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"756-764"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abietic Acid Alleviates the Hypoxic Injury of Cardiomyocytes by Adjusting Autophagy and Apoptosis Mediated by miR-30a-5p/GRP78 Axis.","authors":"Hui Chen, Ming Lu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To explore the influence of abietic acid on the autophagy and apoptosis of cardiomyocytes in rats with acute myocardial infarction (AMI).</p><p><strong>Methods: </strong>A rat model of AMI was built by ligation of the anterior descending branch of left coronary artery, and a model of hypoxic cardiomyocyte injury was constructed by treating cardiomyocytes with hypoxia. Western blot assay was used to detect the abundance of proteins related to autophagy and apoptosis, MTT assay was used to measure the viability of cardiomyocytes, and the expression level of miR-30a-5p was detected by qRT-PCR. The targeting relationship between miR-30a-5p and GRP78 was determined by double luciferase reporter gene experiment.</p><p><strong>Results: </strong>After treatment with abietic acid (315mg/kg), the infarct area of AMI rats was noticeably debased, the intensity of cardiomyocyte autophagy was raised and the level of cardiomyocyte apoptosis was decreased. qRT-PCR results showed that abietic acid inhibited the expression of miR-30a-5p. Furthermore, the dual luciferase reporter gene assay demonstrated that miR-30a-5p targets GRP78. It was found that miR-30a-5p mimic could increase the expression of P62, decrease the LC3II/LC3I ratio, promote the expression of BAX, and reduce the expression of anti-apoptotic protein BCL2, while the effect of GRP78 on cardiomyocytes was opposite to that of miR-30a-5p. After the hypoxic myocardial cells were treated with 50 μM abietic acid, the standard of miR-30a-5p decreased and the abundance of GRP78 increased. After the hypoxic myocardial cells were treated with 50 μM abietic acid, the level of miR-30a-5p decreased and the abundance of GRP78 increased.</p><p><strong>Conclusion: </strong>Abietic acid alleviates the hypoxic injury of cardiomyocytes by adjusting autophagy and apoptosis mediated by miR-30a-5p/GRP78 axis.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"748-755"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143057795","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}