Annals of clinical and laboratory science最新文献

{"title":"miRNA-19a-3p Exacerbates Tube Formation by Targeting SOCS1 to Regulate JAK2/STAT3/VEGF in Diabetic Retinopathy.","authors":"Min Wen, Rong Wu, Jin-Feng Xie, Xiao-Mei Chen, Nian-Lian Wen, Sheng Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Diabetic retinopathy (DR) is a retinal microangiopathy caused by diabetes mellitus. miRNAs have been shown to be involved in DR-associated micro vessel formation, influence the DR progression, and may be a therapeutic strategy for DR. miRNA-19a-3p is highly expressed in the vitreous of DR patients, however, its mechanism in the occurrence and development of DR is unclear. Therefore, the purpose of this study was to explore the potential mechanism of miRNA-19a-3p in the occurrence and development of DR.</p><p><strong>Methods: </strong>Human retinal microvascular endothelial cells (hRMECs) were treated with 30 mM high glucose for 48 h to construct a DR cell model. Quantitative real-time polymerase chain reaction was performed to detect the expression levels of miR-19a-3p and SOCS1; the cell viability was measured by Cell Counting Kit-8 after the interference or overexpression of miR-19a-3p. Cell scratch assay was used to verify the cell migration ability; tube formation assay to measure the tube formation ability. The targeting relationship between miR-19a-3p and SOCS1 was demonstrated by dual-luciferase reporter gene assay. Western blot was adopted to determine the protein expression levels of FGF2, VEGFA, SOCS1, JAK2, p-JAK2, STAT3, and p-STAT3.</p><p><strong>Results: </strong>In the hRMECs induced by high glucose, miRNA-19a-3p was increased, while the expression level of SOCS1 was significantly decreased. The cell viability, migration, and tube formation could be markedly inhibited by suppressing the miR-19a-3p expression in hRMECs under high glucose conditions. Besides, the expression level of SOCS1 was remarkably elevated after the inhibition of miR-19a-3p expression. In addition, miR-19a-3p could directly target the negatively regulated SOCS1. The inhibition of SOCS1 expression reversed the effects of miR-19a-3p down-regulation on the viability, migration, and tube formation of hRMECs under high glucose conditions. miR-19a-3p could activate the JAK2/STAT3 signaling pathway by inhibiting the expression of SOCS1, thereby promoting the tube formation.</p><p><strong>Conclusion: </strong>miRNA-19a-3p can target SOCS1 to regulate JAK2/STAT3/VEGF, thereby exacerbating tube formation in DR. The results of this study provide a new target for the treatment of DR.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"19-27"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal Relationships Between Inflammatory Cytokines and Sepsis: A Mendelian Randomization Study.","authors":"Feng Lu, Cuilan Chen, Dongshan Feng, Zheyi Zhou, Jin Qin, Jiang Qin, Yizhen Yan, Ying Zhong, Xuan Tang, Tingqiu Wei","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>The complex interplay between inflammatory cytokines and sepsis is not well understood. This study employs Mendelian Randomization (MR) to investigate the causal relationships between various inflammatory cytokines and sepsis, aiming to elucidate the underlying mechanisms and potential therapeutic targets.</p><p><strong>Methods: </strong>This study employed a bidirectional MR approach to investigate the causal effects of inflammatory cytokines on sepsis and vice versa. Genetic variants from genome-wide association studies (GWAS) were used as instrumental variables (IVs). Key MR methods included Inverse Variance Weighted (IVW), MR-Egger, and Weighted Median. SNPs were filtered using a p-value threshold of <5e-08, with linkage disequilibrium exclusions (r²<0.001). We analyzed 41 inflammatory cytokines, utilizing leave-one-out analysis and MR-PRESSO to address pleiotropy.</p><p><strong>Results: </strong>The MR analysis revealed significant causal relationships between specific cytokines and sepsis. CTACK (OR=1.102, <i>P</i>=0.031), MIF (OR=1.071, <i>P</i>=0.036), and TRAIL (OR=1.053, <i>P</i>=0.036) were identified as risk factors, while MIP1-β (OR=0.933, <i>P</i>=0.039) and TGF-<i>α</i> (OR=0.957, <i>P</i>=0.029) emerged as protective factors. Additionally, sepsis increased the risk for IL-2 (OR=1.455, <i>P</i><0.01), IL-6 (OR=1.151, <i>P</i>= 0.012), and MCSF (OR=1.272, <i>P</i>=0.028), while showing a protective effect on NGF-β (OR=0.78, <i>P</i>=0.012) and SCF (OR=0.86, <i>P</i>=0.02).</p><p><strong>Conclusion: </strong>This study reveals novel causal relationships between specific inflammatory cytokines and sepsis, suggesting that CTACK, MIF, and TRAIL are risk factors, while MIP1-β and TGF-<i>α</i> are protective. Additionally, sepsis influences various cytokines, indicating complex bi-directional interactions. These findings provide valuable insights for developing targeted therapeutic strategies to manage sepsis and inflammatory responses.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"64-71"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Annals of Clinical and Laboratory Science: Information for Authors.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"145-146"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-Occurrence of <i>ETV6::RUNX1</i> and <i>P2RY8::CRLF2</i> Fusion in a Patient with Relapsed Acute B Lymphoblastic Leukemia.","authors":"William Wu, Chihoon Ahn, Jennifer Scull, Kevin E Fisher, Angshumoy Roy, Dolores H Lopez-Terrada, Andrea N Marcogliese, Anindita Ghosh","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>B-cell acute lymphoblastic leukemias (B-ALLs) harbor recurrent chromosomal aberrations associated with prognostic and therapeutic significance. Although these major driver genetic abnormalities are generally mutually exclusive, they are rarely reported to co-occur. Here, we present the case of a child with B-ALL who suffered two relapses with short event-free intervals. The patient was found to have co-existence of translocation t(12;21) causing <i>ETV6::RUNX1</i> fusion and an interstitial deletion of chromosome X leading to <i>P2RY8::CRLF2</i> fusion. While ETV6::RUNX1 was readily detected by cytogenetic analysis of the bone marrow at the time of diagnosis, the concurrent <i>P2RY8::CRLF2</i> fusion was discovered upon molecular genetic analysis at second relapse. Notably, <i>ETV6::RUNX1</i> and <i>P2RY8::CRLF2</i> each hallmark molecular subclasses of B-ALL with distinct mutational landscapes and clinical prognoses. It is not yet known how co-existing leukemogenic chromosomal aberrations interact to modify treatment response and relapse risk of B-ALL. This case highlights the possibility that co-existing molecular aberrations may greatly impact the clinical course of B-ALL but may be under-recognized in the initial workup using standard diagnostic algorithms.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"133-141"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mucoepidermoid Carcinoma Presented as a Lymph Node Metastasis in 9-Year-Old Child: A Case Report.","authors":"Ahmed Ahmed, Sibel Ak, Sara Amin, Nourhan Ibrahim, Mahmoud Elsayad","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Salivary gland neoplasms are rare in pediatric and adolescent populations. Mucoepidermoid carcinoma (MEC) is the most common malignant neoplasm of the salivary glands that most frequently arises in the parotid gland as a painless swelling. Herein we present a rare case of MEC in a child with metastasis to the cervical lymph node as primary presentation.</p><p><strong>Case report: </strong>A nine-year-old female without significant past medical history presented with a neck mass behind the ear for two months. Imaging study showed a heterogeneous soft tissue mass with a radiologic impression of a suppurative lymph node with surrounding lymphadenopathy. Histological sections of the lymph node showed metastatic carcinoma formed of sheets of epidermoid-looking cells and scattered mucin producing cells (mucocytes). Immunohistochemistry (IHC) study showed that the tumor cells were diffusely positive for Pan-Cytokeratin, p40, and p63 with focal Mucicarmine expression. Follow up positron emission-computed tomography (PET-CT) scan showed a right parotid gland mass-like lesion.</p><p><strong>Conclusion: </strong>MECs are presented in either major or minor salivary glands. The primary presentation of MEC in children and in lymph node is extremely rare. Although the IHC and molecular profiling of the MECs in the pediatric population shows similarities to those tumors observed in the adult population, the overall prognosis in the pediatric group is excellent with approximately 95% five-year overall survival rate.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"128-132"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barriers to Blood Donation: Donor Deferrals in a Saudi Hospital Setting.","authors":"Fahd A Kuriri, Abdulrahman Ahmed, Fahad Alhumud, Fehaid Alanazi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Blood donation plays a critical role in healthcare systems by supplying blood and blood products for surgeries, emergency care, and chronic disease management. In Saudi Arabia, the prevalence of genetic disorders, like sickle cell disease and thalassemia, further increase the need for a stable blood supply. However, high rates of donor deferrals remain a significant barrier to maintaining a reliable blood supply. This study aims to analyse the main factors contributing to blood donor deferrals at King Fahad Hospital Al Hofuf between January 2023 and August 2024. By identifying and correlating key deferral reasons with donor demographics, the study seeks to provide actionable insights to reduce deferral rates and enhance donor retention.</p><p><strong>Methods: </strong>This retrospective observational study analysed blood donation records of 21,149 donors, including both eligible and deferred individuals. Demographic data (age, gender, nationality) and medical assessments (hemoglobin levels, blood pressure) were collected and analysed to identify deferral trends. Descriptive and inferential statistics were applied to assess the relationships between donor characteristics and deferral reasons, with chi-square tests and logistic regression used to identify predictors of deferral.</p><p><strong>Results: </strong>Data analyses showed that 89.5% of deferred donors were male, with the largest group of deferrals aged 21-30 years. Low hemoglobin was the most common deferral reason, affecting 27.5% of all deferred donors, with female donors disproportionately affected by anemia-related deferrals. Male donors were more frequently deferred for aspirin use (13.2%) and blood pressure issues (15.9%). Seasonal trends showed deferral peaks in January and November, while the highest donation rates were recorded in April and October.</p><p><strong>Conclusion: </strong>Addressing the higher rates of deferral due to low hemoglobin and cardiovascular issues through targeted public health interventions could significantly improve donor retention and ensure a more reliable blood supply. Implementing iron supplementation programs for female donors and cardiovascular health screenings for male donors could reduce deferral rates.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"828-836"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-Müllerian Hormone Enhances Migration and Invasion in Human Trophoblast Cells.","authors":"Xuhua Hu, Depeng Zhao, Wanxue Wang, Shuhua Wu, Litong Zhu, Xuemei Li, Zhuomin Huang, Jilong Yao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Anti-Müllerian hormone (AMH) belongs to the transforming growth factor-β superfamily. Recent evidence shows that AMH and its type II receptor (AMHRII) are expressed by the placenta at term. The physiological role of AMH in trophoblast invasion and migration remains to be elucidated.</p><p><strong>Methods: </strong>Immunofluorescence and western blot were used to detect the expression of AMH and AMHRII in trophoblast cell lines and chorionic-decidual mixture from early pregnancies. <i>In vitro</i> experiments, including siRNA transfection, transwell and wound healing assays were further performed to study the function of AMH in trophoblast cell line.</p><p><strong>Results: </strong>AMH and AMHR2 are mainly expressed in the interstitial trophoblast (ITB) in human chorionic-decidual mixture of early pregnancy. We found that knocking down AMH resulted in decreased migration ability of trophoblast cells compared to the control group.</p><p><strong>Conclusion: </strong>It is shown that AMH enhances trophoblast's invasion and migration abilities. AMH may play a role in embryo implantation and placenta formation.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"790-798"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Histoplasmosis in a Solid Organ Transplant Patient in a Non-Endemic Region in New York City.","authors":"Sarah Shidid, Aashna Pandya, Kateryna Karpoff, Tamar A Smith-Norowitz","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>To present the case of a solid organ transplant recipient with Histoplasmosis in New York City.</p><p><strong>Case report: </strong>We present a 39-year-old female liver transplant recipient, who experienced a two-week history of progressive shortness of breath and dyspnea on exertion that interfered with all activities of daily living. Physical examination by the team revealed the patient had a WBC of 11.7, + SARS-CoV-2 NP swab, CT Chest with pneumonia, and a urine antigen test positive for Histoplasmosis. Her overall clinical picture was concerning for new Histoplasmosis infection.</p><p><strong>Conclusion: </strong>Findings endorse the diagnosis of Histoplasmosis. Histoplasmosis is rare in non-endemic regions such as New York City. An infectious workup should include fungal markers to further evaluate shortness of breath and a need to look beyond epidemiologic measures.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"873-876"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Evaluation of Urinary HE4, CEA, ProGRP, CYFRA 21-1 and NSE in the Diagnosis of Lung Cancer.","authors":"Li Zhang, Jing Zhuang, Yuxia Pang, Yinan Song, Xiaofei Li, Kuo Liu, Shuomin Li, Tao Sun","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Urinary proteins are effective tumor biomarkers. Human epididymis protein 4 (HE4), progastrin-releasing peptide (ProGRP), carcinoembryonic antigen (CEA), cytokeratin-19 fragment 21-1(CYFRA 21-1), and neuron-specific enolase (NSE) in serum, were proposed as tumor biomarkers of lung cancer. Our aim was to identify the urine protein biomarkers that can distinguish patients with lung cancer from healthy individuals and/or patients with benign lung disease with a high level of sensitivity and specificity.</p><p><strong>Methods: </strong>These urinary protein concentrations were determined from 212 patients with lung cancer, 80 patients with benign pulmonary conditions, and 100 healthy individuals.</p><p><strong>Results: </strong>In the lung cancer group, urine HE4 median concentration was approximately ten times that in the healthy and/or pulmonary benign disease group; urine CEA, CYFRA21-1, and NSE median concentrations were about eight times, twice, and half as those in the healthy group, respectively. However, the median concentrations of urine ProGRP were almost the same in the healthy, lung benign, and cancer groups. Urine HE4 showed better specificity (94.0% vs 86.0%) and sensibility (72.38% vs 60.65%) than CEA in discriminating patients with lung cancer from healthy controls. A significantly higher ROC area was also obtained with urine HE4 than with CEA (0.87 vs 0.76).</p><p><strong>Conclusions: </strong>Urine HE4 showed the best diagnostic performance, followed by CEA. The levels of urine HE4 and CEA increased significantly in patients with lung cancer, which had little relationship with pathological type and metastasis.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"845-855"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143036162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Patient with a Delayed Diagnosis of Zinc Powder Inhalation Poisoning: A Case Report.","authors":"Min Chai, Xiuxian Zang, Xinran Yang, Chenglin Yu, Bin Nian, Yixin Chen, Dilimulat Maimaiti, Shuai Chen, Dahai Xu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Overexposure to zinc can lead to zinc poisoning. Zinc poisoning from zinc powder inhalation has rarely been reported.</p><p><strong>Case report: </strong>A 66-year-old male presented with nausea, chest tightness, and shortness of breath. He had no symptom improvement after antibiotic treatment. The patient's history revealed recent occupational exposure to cold zinc spray. Bronchoscopy showed a high zinc level (0.5 μmol/L) in the alveolar fluid of the patient. He was diagnosed with zinc powder inhalation poisoning (ZPIP).</p><p><strong>Results: </strong>The patient received supplemental oxygen, high-dose glucocorticoids, hemoperfusion, bronchoalveolar lavage, and supportive care. Eventually, both his symptoms and a repeat lung computed tomography scan showed improvements.</p><p><strong>Conclusion: </strong>ZPIP requires a careful history recording to avoid a missed diagnosis and delayed treatments. We present this case to share our experience regarding the clinical diagnosis of ZPIP and its treatment strategies.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"54 6","pages":"886-890"},"PeriodicalIF":1.1,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143035625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}