Annals of clinical and laboratory science最新文献

{"title":"The HbA1c-Adjusted Slow Valley Height Ratio, a Depth Indicator of the Valley Between HbA1c and HbA0 Measured by High-Performance Liquid Chromatography, Is Useful for the Diagnosis of Hb Toranomon.","authors":"Masafumi Koga, Aki Ishido, Yasushi Inoue, Yoshimi Imai, Hiroko Ijima, Hideaki Jinnouchi, Jun Murai, Mika Ito","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>HbA1c values in patients with Hb Toranomon, a hemoglobin variant, are falsely high when measured with the Tosoh high-performance liquid chromatography (HPLC) analyzer. Moreover, a phenomenon has been observed in which the valley between HbA1c and HbA0 [slow valley (SV)] appears shallow on the chromatograms measured by the Tosoh HPLC analyzer in patients with Hb Toranomon. In the present study, the usefulness of the indicator, HbA1c (A1c)-adjusted SV height rate (AaSVHR), as a screening test for Hb Toranomon to quantify the SV height (SVH) was investigated.</p><p><strong>Methods: </strong>This study included six patients with Hb Toranomon and 100 control participants, comprising 50 diabetic patients and 50 non-diabetic participants. The SVH between HbA1c and HbA0 and the height of HbA1c (A1cH) on HPLC (G9) chromatograms were measured to determine the ratio of SVH to A1cH [SVH ratio (SVHR)]. Given that the SVHR obtained showed a significant negative correlation with HbA1c measured by HPLC, SVHR was corrected with HbA1c [A1c-adjusted SVHR (AaSVHR)], but it showed no significant correlation with HbA1c.</p><p><strong>Results: </strong>AaSVHR exceeded 14% across all participants with Hb Toranomon, but it was no more than 14% in all control participants.</p><p><strong>Conclusion: </strong>AaSVHR measurements are useful as an Hb Toranomon screening test in patients whose HbA1c measured with a Tosoh HPLC analyzer is falsely high. A high AaSVHR exceeding 14% raises a strong suspicion for the presence of a hemoglobin variant including Hb Toranomon.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"108-114"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Two SDS-Based Preparation Methods for Direct Identification and Antifungal Susceptibility Testing of Yeasts from Positive Blood Cultures.","authors":"In Young Yoo, Hada Jang, Miran Lee, Hyun Soo Seok, Yeon-Joon Park","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>We evaluated two SDS-based preparation methods for direct identification of yeasts using MALDI-TOF MS analysis and antifungal susceptibility testing (AFST).</p><p><strong>Methods: </strong>A total of 149 residual monomicrobial blood culture bottles (BCs) were included. For direct identification via MALDI-TOF MS analysis from positive BCs, two in-house methods with or without a 3-hr incubation step were evaluated. 55 samples were also prepared using a Sepsityper kit. In addition, to investigate the effects of differences in <i>Candida</i> spp. concentrations, simulated samples were incubated and colonies were counted. Direct AFST was performed using Sensititre YeastOne.</p><p><strong>Results: </strong>Correct identification rate was 65.8% for the saponin+0.5% SDS method and 70.5% for the 20% SDS after 3-hr incubation, respectively. For the 55 samples evaluated using the Sepsityper kit, both in-house methods showed significantly higher identification rates. For the simulated samples, <i>Candida albicans</i> had the lowest colony counts at the time of the positive signal in the BCs. Essential agreement was above 90% for all cases except for itraconazole using the pellet prepared by the 20% SDS method. Categorical agreement was above 90% for all cases.</p><p><strong>Conclusion: </strong>Our newly developed preparation method using 20% SDS demonstrated superior performance to a commercial kit, and can therefore be used for direct identification and AFST.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"102-107"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression Profiles and Diagnostic Value of Serum Bile Acids in Intrahepatic Cholestasis of Pregnancy.","authors":"Mengjie Xu, Kai Xu, Jun Li, Zibin Wang, Yujie Zhang, Tingting Yu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to ascertain serum bile acid levels and explore the diagnostic value of differential bile acids in patients with intrahepatic cholestasis of pregnancy (ICP).</p><p><strong>Methods: </strong>94 cases of ICP patients admitted to Wenzhou People's Hospital from Oct 2020 to July 2023 were selected as the ICP group, and 184 women who underwent normal prenatal examination in the same period as the healthy control group. The baseline data and 15 kinds of serum bile acid spectrum characteristics of the two groups of pregnant women were compared, and the risk factors of serum bile acid spectrum causing ICP were analyzed by univariate and multivariate logistic regression. A logistic regression model was constructed based on significant risk factors, and ROC curve was drawn to evaluate the diagnostic effect of the model.</p><p><strong>Results: </strong>Compared with the healthy control group, the other 14 serum bile acids except LCA were highly expressed in the ICP group, and there was significant difference (<i>p</i><0.05). Univariate analysis showed that 15 serum bile acids were statistically different from ICP (<i>p</i><0.05). Multivariate analysis showed that both TCA and DCA were risk factors for ICP, with 95% CI of 1.010 (1.003-1.021) and 1.003 (1.000-1.007), respectively. ROC curve showed that TCA, DCA, and TCA+ DCA were the diagnostic criteria of ICP, and their maximum diagnostic value was TCA+ DCA (AUC 0.932). The AUC values of TCA and DCA were 0.929 and 0.615, respectively. TCA+ DCA could play a complementary role in the diagnosis of ICP, and its sensitivity and specificity were 90.7% and 88.9%, respectively.</p><p><strong>Conclusion: </strong>TCA and DCA indicators are closely related to the occurrence of ICP, and the combined detection of TCA and DCA can significantly improve the efficiency of ICP diagnosis.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"72-78"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation Value of Preoperative Serum Transaminase Ratio Combined with Serum Tumor Markers for Prognosis of Patients with Stage III Colorectal Cancer: A Retrospective Study.","authors":"Xiaodong Li, Zhao Wu, Fei Cheng, Lebin Yuan, Weiyang Xia, Zhigang Li, Zeyu Huang, Shengping Mao, Xing Chen, Wei Shen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study utilized data from two hospitals to investigate the relationship between the preoperative serum alanine aminotransferase to aspartate aminotransferase ratio (LSR) combined with preoperative serum tumor markers (TM) and the prognosis of patients with stage III colorectal cancer (CRC).</p><p><strong>Methods: </strong>An analysis was performed on the clinical data of 211 patients diagnosed with stage III colorectal cancer (CRC), treated surgically at the Department of Gastrointestinal Surgery of the Second Affiliated Hospital of Nanchang University and the General Surgery Department of the First Affiliated Hospital of Nanchang University between January 2016 and December 2016. Laboratory data were also compiled for these patients. The main follow-up indicators were the five-year disease-free survival (DFS) and the five-year overall survival (OS). Survival analysis was carried out using the log-rank test, and Cox regression analysis was applied to ascertain independent prognostic factors for survival. Rank sum tests were utilized to compare categorical variables, with a <i>p</i>-value of less than 0.05 considered statistically significant.</p><p><strong>Result: </strong>Between January 2016 and December 2016, a total of 211 patients were enrolled in the two hospitals, including 113 males and 98 females. Multivariate analysis showed that the independent prognostic factors for five-year disease-free survival of stage III colorectal cancer were LSR (HR: 0.45,95% CI:0.31-0.67, <i>p</i><0.001) and CEA (HR: 2.11,95%CI:1.42-3.13, <i>p</i><0.001). Independent prognostic factors for five-year overall survival of stage III colorectal cancer were LSR (HR:0.48, 95%CI:0.31-0.75, <i>p</i>=0.001) and CA199 (HR:1.73, 95%CI:1.11-2.68, <i>p</i>=0.015). Kaplan-Meier survival analysis showed that LSR combined with TM could evaluate the five-year DFS and five-year OS of stage III colorectal cancer (DFS, <i>p</i><0.001, OS: <i>p</i>=0.002).</p><p><strong>Conclusion: </strong>1. The prognosis of patients with high preoperative LSR and low CEA, CA199 in perioperative period is better than that of other stage III colorectal cancer patients. 2. Perioperative preoperative serum transaminase ratio combined with CEA, CA199 has a certain reference value in the prognosis assessment of stage III colorectal cancer.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"54-63"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of Action of microRNA-146a on Lung Cancer Cells through Notch1/Hes-1 Signaling Pathway.","authors":"Hui Xu, Xuan Guo, Ping Xiang, Yang Liu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>We explored the mechanism of action of microRNA-146a in the lung cancer cell line A549 through the Noth1/Hes-1 signal pathway.</p><p><strong>Methods: </strong>A549 cells were divided into a control NT group without transfection, NC group with transfection of miR-146a-NC, and MM group with transfection of miR-146a mimics. We employed qRT-PCR, MTT, Hoechst33258 fluorescence staining, Transwell assay, cell wound scratch assay, and Western blot for detection of the expression of miR-146a and Notch1/Hes-1, cell activity, apoptotic capacity, and invasion and migration abilities.</p><p><strong>Results: </strong>The expression of miR-146a in MM group was the highest, illustrating transfection was successful. The expression of Notch1 and Hes-1 mRNA in MM group was reduced compared to in the NC and NT groups (<i>P</i><0.05). The activity of Notch1 and Hes-1 were reduced by transfection of miR-146a as documented by the double luciferase reporter gene assay (<i>P</i><0.05). The cellular activity in the MM group was lowest after the 48 hours of culture. There was a significant difference compared with the other two groups (<i>P</i><0.05). Cell activity in the three groups after the 96 hours of culture was higher than after the 72 hours and 48 hours of culture equally (<i>P</i><0.05).</p><p><strong>Conclusion: </strong>The low expression of miR-146a could have tumor-prompting action on human NSCLC cells. Up-regulation of the expression of miR-146a restrained biological behaviors such as cell apoptosis and migration through targeted regulation involving the signal pathway of Noth1/Hes-1.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"3-9"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Information About The Association of Clinical Scientists.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"147"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MGLL Promotes Pancreatic Cancer Progression and Invasion: Potential Therapeutic Inhibition by Melatonin.","authors":"Weimin Li, Jin Qian, Hanbo Yang, Jianliang Wu, Zhonglue Wang, Chenghai He, Guodong Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Pancreatic adenocarcinoma (PAAD) is an aggressive and lethal cancer with limited treatment options and poor prognosis. Monoacylglycerol lipase (MGLL), a key enzyme in lipid metabolism, is thought to play a role in tumor progression, but its function in PAAD remains unclear. This study aims to investigate the expression and functional significance of MGLL in pancreatic cancer and evaluate melatonin (MLT) as a potential inhibitor of MGLL.</p><p><strong>Methods: </strong>Tissue samples from 22 PAAD patients were collected and analyzed by immunohistochemistry to assess MGLL expression. Bioinformatics tools, including GEPIA and the Human Protein Atlas, were used to compare MGLL expression in pancreatic cancer versus normal tissue, and survival analysis was conducted. In vitro assays, including CCK-8, colony formation, wound healing, and Transwell, were performed on PANC-1 cells to explore the effects of MGLL knockdown using siRNA. Western blotting was used to analyze the impact of MGLL on the P-AKT/AKT signaling pathway and MMP-2/9 expression. The effect of MLT on MGLL expression was evaluated using RNA-seq and qRT-PCR.</p><p><strong>Results: </strong>MGLL was significantly overexpressed in PAAD tissues and was associated with poor patient prognosis. Silencing MGLL in PANC-1 cells reduced cell proliferation, migration, and invasion, likely via the P-AKT/AKT pathway. MLT treatment effectively downregulated MGLL expression, as well as MMP-2 and MMP-9, suggesting that MLT could serve as a potential MGLL inhibitor.</p><p><strong>Conclusion: </strong>MGLL promotes pancreatic cancer progression through the P-AKT/AKT signaling pathway, and MLT may offer a novel therapeutic strategy by inhibiting MGLL expression. These findings highlight MGLL as a potential therapeutic target for PAAD.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"10-18"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive Evaluation of Red Blood Cell Indices and Their Abnormalities in Cancer Patients.","authors":"Mamdouh Allahyani, Tariq Elmissbah, Magdi Salih, Abdulelah Aljuaid, Meshari A Alsuwat, Mohammed A Alshamrani, Mazen M Almehmadi","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>Cancer is a common cause of mortality and morbidity, and anemia is a major public health issue globally. This study aimed to determine the morphological types of anemia that occur in newly diagnosed patients with solid malignancies.</p><p><strong>Methods: </strong>This retrospective study was performed on a total of 345 cancer patients to record the types of anemia found in patients with solid tumors using calculation of indices (MCV, MCH, and MCHC) in blood gathered from patients with various solid cancers.</p><p><strong>Results: </strong>Data showed that 260 (75.3%) were female, and 85 (24.7%) were male. The age range was 16-97 years. Various types of cancer were enrolled in the study, including breast (98 cases), female genital tract (70 cases), colorectal (86 cases), head and neck (36 cases), bladder (18 cases), prostate (18 cases), lung (eight cases), liver (eight cases), and lymphoma (three cases) cancers. The morphological classification of anemia was as follows: 167 cases (48.4%) normocytic normochromic anemia, 177 (51.3%) microcytic hypochromic anemia; and one (0.3%) macrocytic anemia. The incidence of microcytic hypochromic anemia was very common in patients with colorectal cancer (55 cases) and rare in patients with lung cancer (four cases).</p><p><strong>Conclusion: </strong>Approximately 48.4% and 51.3% of cancer patients had normocytic and microcytic hypochromic anemia, respectively. Increased examination and more intense treatment procedures is required for those individuals with cancer.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"96-101"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adipose Mesenchymal Stem Cell Exosomes Regulate Ferroptosis via ATF3 to Attenuate Acute Myocardial Infarction.","authors":"Linli Chen, Zhao Liu, Lu Wang, Meiyu Gu, Yongli Luo","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>During acute myocardial infarction (AMI), ferroptosis occurs in cardiomyocytes, leading to ventricular remodeling. To investigate the influence of exosomes (Exo) derived from adipose mesenchymal stem cells (AD-MSCs) on cardiomyocytes, a therapeutic approach to resolve AMI may be discovered.</p><p><strong>Methods: </strong>The Exo of AD-MSCs was isolated and identified, and internalized into cardiomyocytes. <i>In vitro</i>, oxygen-sugar deprivation (OGD) was performed to cultivate H9C2 cells to simulate the AMI model, and H9C2 cells incubated with Exo. In addition, an AMI rat model was constructed, and Exo was injected into the edge of myocardial infarction. The levels of MDA, Fe²⁺, GSH, and GPX4 were detected using corresponding kit, the expression levels of ATF3, SLC7A11, PTGS2, and GPX4 were detected using western blot. The oe-ATF3 plasmid was transfected into H9C2 cells to explore the mechanism of ferroptosis regulation by Exo.</p><p><strong>Results: </strong>Exo was isolated and identified successfully, and further confirmed that it could be internalized into H9C2 cells. Compared with the control group, the level of apoptosis and ferroptosis in OGD group was significantly increased, while the level of cell vitality was significantly decreased. After Exo treatment, the level of ferroptosis and apoptosis was significantly decreased, while the level of cell vitality was significantly increased. However, after overexpression of ATF3 in cells, it was found that the ferroptosis level down-regulated by Exo was reversed. Further examination of the SLC7A11/Xct system showed that ATF3 could inhibit the expression of SLC7A11. In addition, compared with the AMI+PBS group, the infarct size of the AMI+Exo group was significantly reduced, and the level of cardiomyocyte apoptosis and ferroptosis was also significantly improved.</p><p><strong>Conclusion: </strong>Exo derived from AD-MSCs can inhibit the expression of ATF3, promote the transport of Fe²⁺ by SLC7A11/Xct system, thus inhibiting the ferroptosis of myocardial cells in AMI, increasing the activity of myocardial cells, and playing a role in alleviating AMI.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"39-47"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The IL-6/STAT3 Signaling Pathway Is Involved in Radiotherapy-Mediated Upregulation of PD-L1 in Esophageal Cancer.","authors":"Yi-Chen Zhou, Hong-Lei Zhu, Xue-Zhou Pang, Yu He, Yang Shen, Dai-Yuan Ma","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the mechanism whereby ionizing radiation improves anti-programmed cell death protein 1 (PD-1)/programmed cell death 1 ligand 1 (PD-L1) immunotherapy for esophageal squamous cell carcinoma (ESCC).</p><p><strong>Methods: </strong>The protein levels of interleukin 6 (IL-6), signal transducer, and activator of transcription 3 (STAT3), and PD-L1 in human ESCC specimens and histologically normal tissues were analyzed by western blot. The effects of radiation exposure on PD-L1, STAT3, and IL-6 were determined in three esophageal cancer (EC) cell lines. Radiation therapy plays an important role in the treatment of esophageal cancer, so cell proliferation, invasion, colony formation, and cell cycle were measured before and after radiation exposure.</p><p><strong>Results: </strong>The protein expression levels of PD-L1, IL-6, and STAT3 were significantly greater in ESCC specimens versus normal tissues. Notably, the protein levels of PD-L1 and STAT3 were positively correlated with the T stage, and IL-6 was positively related to the T stage and tumor site. Cox proportional hazard model multivariate analysis indicated that the expression levels of PD-L1, IL-6, and STAT3 were not independent prognostic factors for ESCC. <i>In vitro</i> studies showed that the PD-L1, STAT3, and IL-6 mRNA and protein expression levels in EC cells exposed to radiation were markedly increased. Further mechanistic investigations found that blocking the IL-6/STAT3 pathway significantly abrogated the radiotherapy-mediated increase in p-STAT3 and PD-L1 levels.</p><p><strong>Conclusion: </strong>Radiotherapy significantly upregulates PD-L1 through the IL-6/STAT3 signaling pathway, suggesting that anti-PD-1/PD-L1 immunotherapy combined with radiotherapy may improve the treatment for ESCC.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"28-38"},"PeriodicalIF":1.1,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143633311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}