Masafumi Koga, Aki Ishido, Yasushi Inoue, Yoshimi Imai, Hiroko Ijima, Hideaki Jinnouchi, Jun Murai, Mika Ito
{"title":"HbA1c调节慢谷高比是高效液相色谱法测定HbA1c与HbA0间谷的深度指标，对Hb异常的诊断有重要意义。","authors":"Masafumi Koga, Aki Ishido, Yasushi Inoue, Yoshimi Imai, Hiroko Ijima, Hideaki Jinnouchi, Jun Murai, Mika Ito","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>HbA1c values in patients with Hb Toranomon, a hemoglobin variant, are falsely high when measured with the Tosoh high-performance liquid chromatography (HPLC) analyzer. Moreover, a phenomenon has been observed in which the valley between HbA1c and HbA0 [slow valley (SV)] appears shallow on the chromatograms measured by the Tosoh HPLC analyzer in patients with Hb Toranomon. In the present study, the usefulness of the indicator, HbA1c (A1c)-adjusted SV height rate (AaSVHR), as a screening test for Hb Toranomon to quantify the SV height (SVH) was investigated.</p><p><strong>Methods: </strong>This study included six patients with Hb Toranomon and 100 control participants, comprising 50 diabetic patients and 50 non-diabetic participants. The SVH between HbA1c and HbA0 and the height of HbA1c (A1cH) on HPLC (G9) chromatograms were measured to determine the ratio of SVH to A1cH [SVH ratio (SVHR)]. Given that the SVHR obtained showed a significant negative correlation with HbA1c measured by HPLC, SVHR was corrected with HbA1c [A1c-adjusted SVHR (AaSVHR)], but it showed no significant correlation with HbA1c.</p><p><strong>Results: </strong>AaSVHR exceeded 14% across all participants with Hb Toranomon, but it was no more than 14% in all control participants.</p><p><strong>Conclusion: </strong>AaSVHR measurements are useful as an Hb Toranomon screening test in patients whose HbA1c measured with a Tosoh HPLC analyzer is falsely high. A high AaSVHR exceeding 14% raises a strong suspicion for the presence of a hemoglobin variant including Hb Toranomon.</p>","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 1","pages":"108-114"},"PeriodicalIF":1.1000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The HbA1c-Adjusted Slow Valley Height Ratio, a Depth Indicator of the Valley Between HbA1c and HbA0 Measured by High-Performance Liquid Chromatography, Is Useful for the Diagnosis of Hb Toranomon.\",\"authors\":\"Masafumi Koga, Aki Ishido, Yasushi Inoue, Yoshimi Imai, Hiroko Ijima, Hideaki Jinnouchi, Jun Murai, Mika Ito\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>HbA1c values in patients with Hb Toranomon, a hemoglobin variant, are falsely high when measured with the Tosoh high-performance liquid chromatography (HPLC) analyzer. Moreover, a phenomenon has been observed in which the valley between HbA1c and HbA0 [slow valley (SV)] appears shallow on the chromatograms measured by the Tosoh HPLC analyzer in patients with Hb Toranomon. In the present study, the usefulness of the indicator, HbA1c (A1c)-adjusted SV height rate (AaSVHR), as a screening test for Hb Toranomon to quantify the SV height (SVH) was investigated.</p><p><strong>Methods: </strong>This study included six patients with Hb Toranomon and 100 control participants, comprising 50 diabetic patients and 50 non-diabetic participants. The SVH between HbA1c and HbA0 and the height of HbA1c (A1cH) on HPLC (G9) chromatograms were measured to determine the ratio of SVH to A1cH [SVH ratio (SVHR)]. Given that the SVHR obtained showed a significant negative correlation with HbA1c measured by HPLC, SVHR was corrected with HbA1c [A1c-adjusted SVHR (AaSVHR)], but it showed no significant correlation with HbA1c.</p><p><strong>Results: </strong>AaSVHR exceeded 14% across all participants with Hb Toranomon, but it was no more than 14% in all control participants.</p><p><strong>Conclusion: </strong>AaSVHR measurements are useful as an Hb Toranomon screening test in patients whose HbA1c measured with a Tosoh HPLC analyzer is falsely high. A high AaSVHR exceeding 14% raises a strong suspicion for the presence of a hemoglobin variant including Hb Toranomon.</p>\",\"PeriodicalId\":8228,\"journal\":{\"name\":\"Annals of clinical and laboratory science\",\"volume\":\"55 1\",\"pages\":\"108-114\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Annals of clinical and laboratory science\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annals of clinical and laboratory science","FirstCategoryId":"3","ListUrlMain":"","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
The HbA1c-Adjusted Slow Valley Height Ratio, a Depth Indicator of the Valley Between HbA1c and HbA0 Measured by High-Performance Liquid Chromatography, Is Useful for the Diagnosis of Hb Toranomon.
Objective: HbA1c values in patients with Hb Toranomon, a hemoglobin variant, are falsely high when measured with the Tosoh high-performance liquid chromatography (HPLC) analyzer. Moreover, a phenomenon has been observed in which the valley between HbA1c and HbA0 [slow valley (SV)] appears shallow on the chromatograms measured by the Tosoh HPLC analyzer in patients with Hb Toranomon. In the present study, the usefulness of the indicator, HbA1c (A1c)-adjusted SV height rate (AaSVHR), as a screening test for Hb Toranomon to quantify the SV height (SVH) was investigated.
Methods: This study included six patients with Hb Toranomon and 100 control participants, comprising 50 diabetic patients and 50 non-diabetic participants. The SVH between HbA1c and HbA0 and the height of HbA1c (A1cH) on HPLC (G9) chromatograms were measured to determine the ratio of SVH to A1cH [SVH ratio (SVHR)]. Given that the SVHR obtained showed a significant negative correlation with HbA1c measured by HPLC, SVHR was corrected with HbA1c [A1c-adjusted SVHR (AaSVHR)], but it showed no significant correlation with HbA1c.
Results: AaSVHR exceeded 14% across all participants with Hb Toranomon, but it was no more than 14% in all control participants.
Conclusion: AaSVHR measurements are useful as an Hb Toranomon screening test in patients whose HbA1c measured with a Tosoh HPLC analyzer is falsely high. A high AaSVHR exceeding 14% raises a strong suspicion for the presence of a hemoglobin variant including Hb Toranomon.
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