Effect of CXCR2 Deficiency in HeLa Cell on the Regulatory Network of Coding Genes and Non-Coding RNAs.

IF 1.1 4区医学 Q4 MEDICAL LABORATORY TECHNOLOGY

Annals of clinical and laboratory science Pub Date : 2024-11-01

Wei Dong, Jifang Gao, Yilin Mu, Yanyan Wang, Xiaoyan Zhu, Jiayin Wang, Chen Zi

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引用次数: 0

Abstract

Objective: C-X-C motif chemokine receptor 2 (CXCR2) plays a crucial role in inflammation and immunity, and the involvement of chemokine receptors in the tumor microenvironment is extensively documented. However, the impact of CXCR2 deficiency on the complete transcriptome, including mRNA and ncRNAs, in tumor cells remains unclear.

Methods: In this study, we aimed to identify differentially expressed (DE) messenger RNA (mRNAs), long non-coding RNAs (lncRNAs), and circular RNAs (circRNAs) in CXCR2 knockout HeLa cells through transcriptome sequencing and to construct regulatory networks.

Results: Compared to control cells, differentially expressed mRNAs, lncRNAs and circRNAs, including 1306 coding genes, 397 lncRNAs, and 846 circRNAs were identified. Understanding these selected genes and ncRNAs could elucidate the influence of CXCR2 on tumor occurrence and progression. Furthermore, 6 DE genes, Rho guanine nucleotide exchange factor 4 (ARHGEF4), calcium voltage-gated channel auxiliary subunit alpha 2 delta 3 (CACNA2D3), fragile histidine triad (FHIT), potassium voltage-gated channel subfamily H member 5 (KCNH5), kelch like family member 1 (KLHL1) and teashirt zinc finger homeobox 2 (TSHZ2), 5 DE lncRNAs, CARMN, AL357060.1, AC098487.1, CECR7 and XLOC_009647, and 9 DE circRNAs, hsa_circ_0000196, hsa_circ_0000234, hsa_circ_0007976, hsa_circ_0008798, hsa_circ_0007766, hsa_circ_0116612, hsa_circ_0008012, hsa_circ_0004576 and hsa_circ_0118105 were verified using real-time PCR and sanger sequencing.

Conclusion: Transcriptome analysis revealed that CXCR2 deficiency in HeLa cells alters the expression of coding genes and ncRNAs involved in tumor and cytokine signaling. Overall, our findings offer potential insights into the mechanisms and new research targets for cervical cancer and other tumors.

本刊更多论文

HeLa细胞CXCR2缺失对编码基因和非编码rna调控网络的影响

目的：C-X-C基序趋化因子受体2 （CXCR2）在炎症和免疫中起着至关重要的作用，趋化因子受体在肿瘤微环境中的参与已被广泛报道。然而，CXCR2缺乏对肿瘤细胞中完整转录组（包括mRNA和ncrna）的影响尚不清楚。方法：在本研究中，我们旨在通过转录组测序鉴定CXCR2敲除HeLa细胞中的差异表达（DE）信使RNA （mrna）、长链非编码RNA （lncRNAs）和环状RNA (circRNAs)，并构建调控网络。结果：与对照细胞相比，鉴定出差异表达的mrna、lncrna和circrna，包括1306个编码基因、397个lncrna和846个circrna。了解这些被选择的基因和ncrna可以阐明CXCR2对肿瘤发生和进展的影响。此外，6个DE基因，Rho鸟苷核苷酸交换因子4 (ARHGEF4)，钙电压门控通道辅助亚基α 2 δ 3 (CACNA2D3)，脆弱组氨酸三联体（FHIT），钾电压门控通道亚家族H成员5 (KCNH5)， kelch样家族成员1 （KLHL1）和teasht锌指同源盒2 (TSHZ2)， 5个DE lncRNAs， CARMN, AL357060.1, AC098487.1， CECR7和XLOC_009647, 9个DE circRNAs， hsa_circ_0000196, hsa_circ_0000234, hsa_circ_0007976, hsa_circ_0008798，hsa_circ_0007766、hsa_circ_0116612、hsa_circ_0008012、hsa_circ_0004576和hsa_circ_0118105采用实时荧光定量PCR和sanger测序进行验证。结论：转录组分析显示，HeLa细胞中CXCR2缺失改变了与肿瘤和细胞因子信号传导相关的编码基因和ncrna的表达。总的来说，我们的发现为宫颈癌和其他肿瘤的机制和新的研究靶点提供了潜在的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of clinical and laboratory science 医学-医学实验技术

CiteScore

1.60

自引率

0.00%

发文量

112

审稿时长

6-12 weeks

期刊介绍： The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.