Downregulation of microRNA-129-2 by Hepatitis B Virus Promotes Cell Proliferation in Hepatocellular Carcinoma.

IF 1.1 4区 医学 Q4 MEDICAL LABORATORY TECHNOLOGY
Laipeng Liu, Pingfeng Zhang, Sheng Sun, Chuanpei Cao, Zhi Song
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引用次数: 0

Abstract

Objective: MicroRNA-192-2 has been shown to have a role in the early diagnosis of hepatocellular carcinoma (HCC), but the relationship between microRNA-192-2 and hepatitis B virus (HBV) in HCC patients remains unclear.

Methods: Specimens were collected from 56 HCC patients diagnosed with HBV infection and 56 HCC patients without viral infection. HBV and miR-129-2 levels in HCC tissues, adjacent tissues, and cell lines were analyzed by RT‒PCR. miR-129-2 mimics were transfected to induce the overexpression of miR-129-2 and cell function was assessed by a wound healing test. Tumor formation experiments in nude mice were conducted to validate tumor proliferation.

Results: In HCC patients, miR-192-2 was significantly downregulated in tumor tissues compared to adjacent tissues. Notably, miR-192-2 level was even lower in HCC patients with HBV-infection than those without the viral infection. Moreover, there was a negative correlation between miR-192-2 and HBV levels. Regardless of HBV infection, patients with low miR-192-2 levels had poorer prognosis than those with high miR-192-2 levels. HBV infection suppressed miR-192-2 expression in HCC cell lines. Furthermore, overexpression of miR-192-2 significantly inhibited cell proliferation both in vitro and in vivo in the HBV-infected group.

Conclusion: Our study revealed that the expression of miR-192-2, a crucial tumor suppressor gene, was suppressed by the presence of HBV.

乙型肝炎病毒下调 microRNA-129-2 促进肝细胞癌细胞增殖
目的:微RNA-192-2已被证明在肝细胞癌(HCC)的早期诊断中发挥作用,但HCC患者体内的微RNA-192-2与乙型肝炎病毒(HBV)之间的关系仍不清楚:方法:从 56 例确诊感染 HBV 的 HCC 患者和 56 例未感染病毒的 HCC 患者中采集标本。通过 RT-PCR 分析了 HCC 组织、邻近组织和细胞系中的 HBV 和 miR-129-2 水平。转染 miR-129-2 模拟物以诱导 miR-129-2 的过表达,并通过伤口愈合试验评估细胞功能。在裸鼠体内进行肿瘤形成实验,以验证肿瘤的增殖情况:结果:在 HCC 患者中,与邻近组织相比,miR-192-2 在肿瘤组织中明显下调。值得注意的是,有 HBV 感染的 HCC 患者的 miR-192-2 水平甚至低于没有病毒感染的患者。此外,miR-192-2 与 HBV 水平呈负相关。无论是否感染 HBV,miR-192-2 水平低的患者预后都比 miR-192-2 水平高的患者差。HBV 感染抑制了 miR-192-2 在 HCC 细胞系中的表达。此外,在 HBV 感染组中,miR-192-2 的过表达能显著抑制体外和体内的细胞增殖:我们的研究表明,HBV 的存在抑制了重要的肿瘤抑制基因 miR-192-2 的表达。
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来源期刊
Annals of clinical and laboratory science
Annals of clinical and laboratory science 医学-医学实验技术
CiteScore
1.60
自引率
0.00%
发文量
112
审稿时长
6-12 weeks
期刊介绍: The Annals of Clinical & Laboratory Science welcomes manuscripts that report research in clinical science, including pathology, clinical chemistry, biotechnology, molecular biology, cytogenetics, microbiology, immunology, hematology, transfusion medicine, organ and tissue transplantation, therapeutics, toxicology, and clinical informatics.
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