Annals of clinical and laboratory science最新文献_第6页

Diagnostic Value of Novel Coupled Hematological-Biochemical Indices in Dengue Virus Infection Beyond Single-Parameter Assessment: A Pilot Study. 新型血液学-生化耦合指标对登革热病毒感染的诊断价值超越单参数评估：一项初步研究。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2026-03-01

Ahmad Shaikh, Mofareh Alabdaly

{"title":"Diagnostic Value of Novel Coupled Hematological-Biochemical Indices in Dengue Virus Infection Beyond Single-Parameter Assessment: A Pilot Study.","authors":"Ahmad Shaikh, Mofareh Alabdaly","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Severe dengue pathogenesis involves complex interactions among hepatic injury, hemostatic failure, and endothelial dysfunction processes. This study aimed to develop and assess a panel of novel hematological-biochemical coupling indices for early risk assessment in patients with dengue virus infection.Method: In this retrospective study, we analyzed 30 patients with confirmed dengue virus infection and 35 age- and sex-matched healthy controls. A set of eight composite indices was calculated from routine admission laboratory data; these comprised the Mean Platelet Volume to Platelet Count Ratio (MPR), Aspartate Aminotransferase-to-Platelet Ratio Index (APRI), Fibrosis-4 (FIB-4), Aspartate Aminotransferase-to-Lymphocyte Ratio Index (ALRI), Coagulo-Inflammatory Index (CII), Platelet-Coagulation Stress Index (PCSI), Hepato-Platelet Injury Index (HPII), and Dengue Plasma Leakage Index (DPLI). Receiver operating characteristic curve analysis and multivariable logistic regression were performed to evaluate the diagnostic accuracy and independent predictive value of each index.Results: All indices were significantly elevated in dengue patients compared to controls (p<0.001) and demonstrated excellent discriminative ability, with area under the curve values ranging from 0.846 to 1.000 (p<0.001). Notably, MPR, APRI, and PCSI showed exceptionally high odds ratios, reflecting the profound multi-system dysregulation characteristic of dengue (p<0.001).Conclusion: The novel composite indices MPR, APRI, FIB-4, ALRI, CII, PCSI, HPII, and DPLI exhibit outstanding diagnostic performance for dengue virus infection.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"159-168"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Systemic Immune-Inflammation Index as a Prognostic Marker in Mycosis Fungoides. 全身免疫炎症指数作为蕈样真菌病的预后指标。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2026-03-01

Gökçe Işıl Kurmuş, Dilek Menteşoğlu, Selda Pelin Kartal

{"title":"Systemic Immune-Inflammation Index as a Prognostic Marker in Mycosis Fungoides.","authors":"Gökçe Işıl Kurmuş, Dilek Menteşoğlu, Selda Pelin Kartal","doi":"","DOIUrl":"","url":null,"abstract":"Objective: The systemic immune-inflammation index (SII) is considered a prognostic factor in numerous malignancies and is associated with disease progression. Although systemic inflammation has been investigated in mycosis fungoides (MF), the prognostic significance of SII remains unclear. This study aimed to evaluate the association between SII and disease stage in MF and to determine whether elevated SII values are related to advanced-stage disease.Methods: A retrospective analysis was performed on 126 patients diagnosed with MF at a tertiary center. The prognostic utility of SII was assessed alongside neutrophil-to-lymphocyte ratio, lymphocyte-to-monocyte ratio, and clinicopathological variables including age, sex, disease stage, lactate dehydrogenase, and beta-2 microglobulin levels.Results: Receiver operating characteristic curve analysis demonstrated that SII had the highest area under the curve (AUC) among the evaluated markers. For distinguishing MF patients from controls, SII yielded an AUC of 0.828 at a cut-off value >410.61 (sensitivity 70.63%, specificity 84.40%). In differentiating early and advanced MF stages, SII showed the highest diagnostic accuracy (AUC 0.891, p<0.001; sensitivity 80.95%, specificity 88.57%) with an optimal cut-off value of 891.02.Conclusions: SII is a cost-effective and accessible prognostic marker for advanced-stage MF and may aid in identifying high-risk patients who benefit from closer monitoring or earlier intervention.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"144-151"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of IL-33 in Upregulating AREG Production During Wound Healing. IL-33在伤口愈合过程中上调AREG生成中的作用。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2026-03-01

Ruichang Zhang, Lin Zhou, Zhuo Zhang, Wenbo Liu

{"title":"Role of IL-33 in Upregulating AREG Production During Wound Healing.","authors":"Ruichang Zhang, Lin Zhou, Zhuo Zhang, Wenbo Liu","doi":"","DOIUrl":"","url":null,"abstract":"Objective: IL-33 plays an important role in regulating immune responses and tissue repair; however, the specific mechanisms of IL-33 in wound healing, particularly in activating regulatory T cells (Tregs), remain unclear. This study aimed to investigate the role of IL-33 in promoting wound healing by regulating amphiregulin (AREG) production in Treg cells.Methods: Kunming mice and Areg knockout (Areg-/-) mice were used to establish full-thickness skin wound models. Recombinant mouse IL-33 (rmIL-33) was administered intranasally or intradermally to assess wound healing dynamics. Wound tissues were analyzed via hematoxylin-eosin (HE) staining, Masson's trichrome staining, flow cytometry, quantitative real-time PCR (qRT-PCR), and enzyme-linked immunosorbent assay (ELISA). Clinical samples from wound-healing patients and healthy controls were evaluated for AREG expression in CD4+ T cells by flow cytometry and qRT-PCR.Results: rmIL-33 treatment significantly enhanced Treg accumulation and AREG secretion in wound tissues, accelerating wound closure, collagen deposition, and re-epithelialization in wild-type mice. Areg deficiency markedly delayed healing, attenuated inflammatory cytokine regulation (TNF-α, TGF-β, IL-1β, IL-6), and reduced collagen synthesis (COL1, COL3). Recombinant TGF-β restored the wound-healing-promoting effects of Areg by reversing collagen metabolic disorders. Clinically, wound-healing patients showed increased AREG expression in skin tissues and Treg cells compared to controls.Conclusion: IL-33 promotes wound healing by activating Tregs to secrete AREG, which in turn regulates tissue repair via the TGF-β signaling pathway. These findings highlight the IL-33/Treg/AREG/TGF-β axis as a potential therapeutic target for enhancing wound healing.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"169-181"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composite CLL/SLL and Lymphoplasmacytic Lymphoma with IgM Anti-MAG Neuropathy: Clinicopathologic and Molecular Characterization of a Diagnostically Challenging Case. 复合CLL/SLL和淋巴浆细胞性淋巴瘤合并IgM抗mag神经病变：一个诊断具有挑战性的病例的临床病理和分子特征。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2026-03-01

Ruimeng Yang, Matthew T Ye, Caroline G Medeiros, Fengxi Ye, M James You

{"title":"Composite CLL/SLL and Lymphoplasmacytic Lymphoma with IgM Anti-MAG Neuropathy: Clinicopathologic and Molecular Characterization of a Diagnostically Challenging Case.","authors":"Ruimeng Yang, Matthew T Ye, Caroline G Medeiros, Fengxi Ye, M James You","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Composite lymphoma, defined by the coexistence of two or more distinct lymphoma types at the same anatomic site, is rare and diagnostically challenging. Composite chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) and lymphoplasmacytic lymphoma (LPL) has been reported only rarely.Case report: A 75-year-old woman with long-standing CLL/SLL developed progressive sensorimotor neuropathy. Serum studies detected elevated IgM (988 mg/dL), an IgM lambda paraprotein (0.5 g/dL), and IgM anti-myelin-associated glycoprotein antibody. Imaging studies identified a dominant pelvic lymph node. Biopsy revealed two B-cell neoplasms: a predominant CLL/SLL component (CD5+, CD23+, CD200+, dim monotypic lambda, CD10-), and a minor LPL component (CD38+, bright monotypic lambda, CD5-, CD10-, CD23-, CD200-). Targeted sequencing detected MYD88 L252P mutation in peripheral blood and pelvic lymph node, supporting LPL. The patient was treated with intrathecal methotrexate and cytarabine together with zanubrutinib, which led to neurological improvement and clearance of CLL/SLL cells from cerebrospinal fluid.Conclusions: This case highlights the diagnostic complexity of composite indolent B-cell lymphomas and underscores the importance of integrated clinical, morphologic, immunophenotypic, and molecular assessment to inform management and surveillance.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 2","pages":"239-244"},"PeriodicalIF":1.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148203782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Review: Differentiating Various Renal Cell Tumors with Clear Cytoplasmic Features, Old and New, Based on Their Different Renal Tubular Origins. 综述：基于不同肾小管起源鉴别具有明确细胞质特征的各种肾细胞瘤的新旧。

IF 0.9 4区医学

Annals of clinical and laboratory science Pub Date : 2026-02-03

Kevin J Zhang, Jason M Hafron, Catherine Xie, Shivam M Patel, Brandon M Metcalf, Ping L Zhang

{"title":"Review: Differentiating Various Renal Cell Tumors with Clear Cytoplasmic Features, Old and New, Based on Their Different Renal Tubular Origins.","authors":"Kevin J Zhang, Jason M Hafron, Catherine Xie, Shivam M Patel, Brandon M Metcalf, Ping L Zhang","doi":"","DOIUrl":"","url":null,"abstract":"Clear cell renal cell carcinoma (ccRCC) is the prototypical renal tumor with clear cell features in which the clear cell cytoplasm results from the melted lipid of the cytoplasm after fixation. ccRCC can be associated with a high risk of tumor metastasis.Methods: In recent years, several new renal mass entities such as RCC with fibromyomatous stroma (FMS), clear cell papillary renal cell tumor (CCP-RCT), multilocular cystic renal cell neoplasm of low malignancy potential (MCRCNLMP), and eosinophilic vacuolated tumor (EVT) have been recognized with some clear-appearing cytoplasm.Results: Previously identified renal tumor entities like chromophobe renal cell carcinoma (Ch-RCC) and translocation renal cell carcinoma (tRCC) often have clear cytoplasmic features as well. While often challenging due to their morphologic similarities, differentiating these renal tumor entities from ccRCC is important given their varying biological behaviors.Conclusion: This review will provide a comprehensive roadmap to pathologically differentiate the variants of renal masses with clear cytoplasmic features. In addition, their clinical outcomes and management will be briefly discussed.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"845-858"},"PeriodicalIF":0.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Monoclonal Antibody against hIL18 Receptor Accessory Protein Chain Promotes Anti-Tumor Activity in Triple-Negative Breast Cancer. 人抗hIL18受体辅助蛋白链单克隆抗体促进三阴性乳腺癌的抗肿瘤活性

IF 0.9 4区医学

Annals of clinical and laboratory science Pub Date : 2026-02-03

Fang Hu, Yonggang Wang, Guowei Qian, Wenxi Yu, Lina Tang, Feng Lin

{"title":"Human Monoclonal Antibody against hIL18 Receptor Accessory Protein Chain Promotes Anti-Tumor Activity in Triple-Negative Breast Cancer.","authors":"Fang Hu, Yonggang Wang, Guowei Qian, Wenxi Yu, Lina Tang, Feng Lin","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that lacks effective targeted therapies. The interleukin-18 (IL-18) signaling pathway contributes to cancer progression and poor survival outcomes in several tumor types, including TNBC. IL-18 receptor accessory protein chain (IL-18RAcP), binding to the IL18/IL18Rα complex, plays a critical role in initiating and transducing IL-18 signaling. Targeting IL-18RAcP with human antibodies may offer a promising therapeutic strategy for TNBC.Methods: Human single-chain variable fragment (scFv) antibodies targeting IL-18RAcP were screened via phage display and characterized using ELISA, SPR, and crystallography. The functional assays that were used included qRT-PCR, MTT, Western blotting, flow cytometry, and xenograft models. Binding interactions were further validated through yeast two-hybrid assays and structural analysis.Results: We found that elevated IL-18RAcP expression in TNBC is associated with poor recurrence-free survival (RFS), suggesting its potential as both a clinical marker and a therapeutic target. Using combinatorial scFv antibody phage display libraries, we identified a human monoclonal antibody, scFvAPC10, which binds IL-18RAcP with high affinity. scFvAPC10 significantly inhibits TNBC cell proliferation in vitro and reduces tumor growth in vivo by inducing apoptosis. Mechanistic studies reveal that scFvAPC10 impairs both NF-κB and MAPK signaling pathways. Structural analysis shows that scFvAPC10 interacts with the D1-D2 domains of IL-18RAcP through three hydrogen bonds, confirming the specificity of the interaction.Conclusions: Our findings highlight the therapeutic potential of targeting IL-18RAcP in TNBC through modulation of IL-18/IL-18R-mediated signaling pathways. The development of scFvAPC10 offers a promising approach for novel antibody-based therapies in the treatment of TNBC.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"55 6","pages":"906-919"},"PeriodicalIF":0.9,"publicationDate":"2026-02-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146112091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Information About The Association of Clinical Scientists. 关于临床科学家协会的信息。

IF 1 4区医学

Annals of clinical and laboratory science Pub Date : 2026-01-01

引用次数: 0

Coinfection of Human Cytomegalovirus and Pneumocystis jirovecii Caused Severe Pneumonia in a Non-HIV Elderly Patient: A Case Report. 非hiv老年人巨细胞病毒与肺囊虫合并感染致重症肺炎1例。

IF 0.9 4区医学

Annals of clinical and laboratory science Pub Date : 2026-01-01

Hui Huang, Zhongda Liu, Xiaojing Liu, Quan Li, Xiaoming Yang, Lingyong Chen, Wenqian Ye

引用次数: 0

Tertiary Lymphoid Structures Predict Efficacy of Neoadjuvant Immunochemotherapy in Esophageal Squamous Cell Carcinoma. 三级淋巴样结构预测食管鳞状细胞癌新辅助免疫化疗的疗效。

IF 0.9 4区医学

Annals of clinical and laboratory science Pub Date : 2026-01-01

Wenwen Rong, Fuhao Xu, Shuanghu Yuan

{"title":"Tertiary Lymphoid Structures Predict Efficacy of Neoadjuvant Immunochemotherapy in Esophageal Squamous Cell Carcinoma.","authors":"Wenwen Rong, Fuhao Xu, Shuanghu Yuan","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Neoadjuvant immunochemotherapy holds tremendous potential for patients with esophageal squamous cell carcinoma (ESCC); however, effective biomarkers for predicting its efficacy are lacking. This study explored the correlation between tertiary lymphoid structures (TLSs) with pathological response and disease-free survival (DFS) to predict the efficacy of neoadjuvant immunochemotherapy in ESCC.Methods: We enrolled 104 patients with ESCC who underwent neoadjuvant immunochemotherapy and radical surgery. We retrospectively analyzed the general clinical data, pre-surgery blood routine results, and post-surgery pathological data of all participants. TLSs in ESCC tissue sections were determined using hematoxylin-eosin staining. Univariate chi-square and multivariate logistic analyses were used to determine the correlation of hematological parameters and TLSs with major pathological response (MPR). The Kaplan-Meier method, along with univariate and multivariate Cox regression analyses, were employed to evaluate the prognostic significance of the studied parameters on DFS.Results: TLSs were significantly associated with the efficacy of neoadjuvant immunochemotherapy in patients with resectable ESCC. Logistic analysis found that TLSs positivity (odds ratio OR=3.252, p=0.039) and platelet-to-lymphocyte ratio<173.155 (OR=0.393, p=0.025) were independent predictors of MPR. Cox regression analyses identified TLSs (HR=0.318, p=0.008) as an independent prognostic factor for patients with ESCC.Conclusion: In patients with ESCC, TLSs are promising indicators for determining the efficacy of neoadjuvant immunochemotherapy.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 1","pages":"96-106"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Indoleamine 2,3-dioxygenase Suppresses Neutrophilic Airway Inflammation by Regulating Th17 and Treg Responses. 吲哚胺2,3-双加氧酶通过调节Th17和Treg反应抑制中性粒细胞气道炎症。

IF 0.9 4区医学

Annals of clinical and laboratory science Pub Date : 2026-01-01

Zhiqiang Chen, Ying Hu, Jing Zeng, Na Cai, Shouyan Zheng, Wei Liao

{"title":"Indoleamine 2,3-dioxygenase Suppresses Neutrophilic Airway Inflammation by Regulating Th17 and Treg Responses.","authors":"Zhiqiang Chen, Ying Hu, Jing Zeng, Na Cai, Shouyan Zheng, Wei Liao","doi":"","DOIUrl":"","url":null,"abstract":"Objective: Th17 cells play an important role in the promotion of asthma inflammation. Indoleamine 2,3-dioxygenase (IDO)-dependent tryptophan metabolism has been shown to act as a molecular \"switch\" for the conversion of Th17 cells into Tregs under certain conditions.Methods: A neutrophilic asthma model was established using ovalbumin (OVA) and lipopolysaccharide. IDO expression in the model mice was regulated using an IDO inducer and an IDO inhibitor. Th17 cells and the secretion of related factors were examined, and changes in airway hyperreactivity and inflammation were observed. Plasmacytoid dendritic cells and naïve CD4+ T cells were co-cultured in vitro. After OVA stimulation and IDO inhibitor treatment, changes in Th17 cells and the secretion of related factors were examined.Results: Airway hyperreactivity and inflammation were ameliorated in the neutrophilic asthma model mice in the IDO inducer group. Compared to the asthma model group, the IDO inducer group showed decreased Th17 cell percentages, increased Treg cell percentages in the peripheral blood, and reduced levels of IL-17, IL-6, and TGF-β1 in the BALF and lung tissues; IDO inhibited RORγt expression and promoted Foxp3 expression in the neutrophilic asthma model. Moreover, IDO inhibited DC-mediated Th17 cell differentiation and secretion of related cytokines, whereas these effects were reversed by the IDO inhibitor.Conclusion: IDO ameliorated airway hyperreactivity and inflammation in neutrophilic asthma by inhibiting Th17 cells and inducing Treg production. These results will provide a new basis for developing potential therapeutic targets for the prevention and treatment of neutrophilic asthma using IDO.","PeriodicalId":8228,"journal":{"name":"Annals of clinical and laboratory science","volume":"56 1","pages":"66-75"},"PeriodicalIF":0.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147607792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0